ANTI-αvβ6 ANTIBODIES AND ANTIBODY-DRUG CONJUGATES

Abstract

To provide novel anti-αvβ6 antibodies and antibody-drug conjugates, and methods of using such anti-αvβ6 antibodies and antibody-drug conjugates to treat cancer.SOLUTION: Preferred anti-αvβ6 antibodies comprise CDR-H1, CDR-H2 and CDR-H3 that comprise respective specific sequences, and CDR-L1, CDR-L2 and CDR-L3 that comprise respective specific sequences.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Patent Application No. 62 / 943,959, filed December 5, 2019, and U.S. Provisional Patent Application No. 63 / 012,584, filed April 20, 2020, each of which is incorporated by reference in its entirety for all purposes.

[0002] Reference to sequence listing This application contains an electronic sequence listing in file named AVB6-00212_ST25, which was created on November 16, 2020, and contains 52 KB, and is incorporated herein by reference.

[0003] The present invention relates to novel anti-αvβ6 antibodies and antibody-drug conjugates, and methods of using such anti-αvβ6 antibodies and antibody-drug conjugates for treating cancer. [Background technology]

[0004] αvβ6, also known as alpha-v beta-6, is a cell adhesion receptor that binds to extracellular matrix proteins such as fibronectin. αvβ6 is composed of alpha v and beta 6 subunits and is upregulated in multiple cancers, including non-small cell lung cancer (NSCLC).

[0005] NSCLC is the most common type of lung cancer. More than 200,000 people were diagnosed with lung cancer last year, and it is the leading cause of cancer death. Therefore, improved treatments for NSCLC are needed.

[0006] All references cited herein, including patent applications, patent publications, and scientific literature, are incorporated by reference in their entirety as if each individual reference was specifically and individually indicated to be incorporated by reference. Summary of the Invention

[0007] Anti-αvβ6 antibodies and αvβ6-directed antibody drug conjugates (ADCs) are provided herein. Methods of using anti-αvβ6-directed antibodies and ADCs to treat αvβ6-expressing disorders, including cancer, are also provided herein. Preferred anti-αvβ6 antibodies include heavy chain CDR sequences of SEQ ID NOs: 31, 32, and 33 and light chain CDR sequences of SEQ ID NOs: 37, 42, and 39, as determined by Kabat numbering. [Brief description of the drawings]

[0008] [Figure 1] FIG. 1 shows the results of a LAP-masking ELISA assay using various anti-αvβ6 antibodies. [Diagram 2] FIG. 1 shows the results of a saturation binding study of 293F cells expressing human and cynomolgus monkey (cyno) αvβ6 with the murine antibody clone 2A2 (designated m2A2). [Diagram 3] FIG. 1 shows an alignment of the amino acid sequence of the heavy chain variable region of the parental murine mAb (designated m2A2) with the selected human germline acceptor (designated hIGHV1-46 / HJ4) and humanized 2A2 heavy chain variants. Asterisks represent CDRs determined by Kabat and crosses represent CDRs determined by IMGT. [Figure 4] FIG. 1 shows an alignment of the amino acid sequences of the parental murine mAb (designated m2A2) light chain variable region with selected human germline acceptor (designated hIGKV1D-33 / KJ2) and humanized 2A2 light chain variants. Asterisks represent CDRs as determined by Kabat and crosses represent CDRs as determined by IMGT. [Diagram 5] FIG. 1 shows the results of competitive binding studies in 293F cells expressing human αvβ6 with humanized antibodies with LA and LB light chains and the parental murine and chimeric antibodies (designated m2A2 and c2A2, respectively). [Figure 6]FIG. 1 shows the results of competitive binding studies in 293F cells expressing human αvβ6 with humanized antibodies with HA and HC heavy chains and the parental murine and chimeric antibodies (designated m2A2 and c2A2, respectively). [Figure 7] FIG. 1 shows the results of repeating competitive binding studies in 293F cells expressing human αvβ6 with a subset of humanized antibodies and the parental murine antibody (designated m2A2). [Figure 8] FIG. 1 shows the results of a saturation binding study of 293F cells expressing human and cynomolgus monkey (cyno) αvβ6 together with the h2A2 HCLG humanized antibody and the parental murine antibody (designated m2A2). [Figure 9] FIG. 1 shows the results of competitive binding studies in 293F cells expressing human and cynomolgus monkey (cyno) αvβ6 together with HCLG humanized antibodies and ADCs. [Figure 10] FIG. 13 shows the results of αvβ6-specific binding studies by ELISA to human αvβ1, αvβ3, αvβ5, αvβ6, and αvβ8 using h2A2 HCLG humanized antibody. [Figure 11] FIG. 13 shows that h2A2 HCLG anti-αvβ6 vcMMAE ADC exhibits in vitro cytotoxicity against αvβ6-expressing cancer cell lines. [Figure 12]

[0023] Figure 1 shows the results of a xenograft study of the Detroit 562 head and neck cancer cell line in nude mice, with doses and schedules indicated in the figure. [Figure 13]

[0023] Figure 1 shows the results of a xenograft study of the HPAFII pancreatic cancer cell line in nude mice, with doses and schedules indicated in the figure. [Figure 14]

[0023] Figure 1 shows the results of a xenograft study of the BxPC-3 pancreatic cancer cell line in nude mice, with doses and schedules indicated in the figure. [Figure 15]

[0023] Figure 1 shows the results of a xenograft study of SW780 bladder cancer cell line in nude mice, with doses and schedules indicated in the figure. [Figure 16]FIG. 1 shows the results of a PDX study in six NSCLC cell lines in nude mice. ADC was administered at 3 mg / kg q7dx3. [Figure 17] FIG. 1 shows the results of a PDX study in six ovarian cancer cell lines in nude mice. ADC was administered at 5 mg / kg q7dx3. [Figure 18] FIG. 1 shows the results of a comparison between h2A2 HCLG and h15H3 in a two cell line xenograft model. ADC was administered once at 3 mg / kg. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0009] I. Definition In order that this disclosure may be more readily understood, certain terms are first defined. As used in this application, unless expressly stated otherwise herein, each of the following terms shall have the following meaning. Additional definitions are set forth throughout this application.

[0010] The term "and / or" as used herein should be interpreted as a specific disclosure of each of the two specified features or components with or without the other. Thus, the term "and / or" used in phrases herein, such as "A and / or B", is intended to include "A and B", "A or B", "A" (single) and "B" (single). Similarly, the term "and / or" used in phrases, such as "A, B, and / or C", is intended to include each of the following aspects: A, B, and C; A, B, or C; A or C; A or B; B or C; A and C; A and B; B and C; A alone; B alone; and C alone.

[0011] It is understood that the aspects and embodiments of the invention described herein include those aspects and embodiments that "comprise", "consist" and "consist essentially of".

[0012] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which this disclosure pertains. For example, the Concise Dictionary of Biomedicine and Molecular Biology, Juo, Pei-Show, 2nd ed., 2002, CRC Press; The Dictionary of Cell and Molecular Biology, 3rd ed., 1999, Academic Press; and the Oxford Dictionary Of Biochemistry And Molecular Biology, Revised, 2000, Oxford University Press provide those of ordinary skill in the art with a general dictionary of many of the terms used in this disclosure.

[0013] Units, prefixes, and symbols are indicated in the format accepted by the International System of Units (SI). Numerical ranges are inclusive of the numerical values ​​defining the range. The headings provided herein are not limitations of the various aspects of the disclosure, but may be had by reference to the specification as a whole. Thus, the terms defined immediately below are more fully defined by reference to the specification as a whole.

[0014] The terms "αvβ6", "avb6", "alpha-v beta-6", or "β6" are used interchangeably herein and, unless otherwise specified, include any variants, isoforms and species homologs of human αvβ6 that are generally expressed by or expressed on cells transfected with the αvβ6 gene.

[0015] The term "immunoglobulin" refers to a class of structurally related glycoproteins consisting of two pairs of polypeptide chains, a pair of light (L) low molecular weight chains and a pair of heavy (H) chains, all of which are interconnected by disulfide bonds. The structure of immunoglobulins is well characterized; see, e.g., Fundamental Immunology, Chapter 7 (Paul, W., ed., 2nd ed., Raven Press, NY (1989)). Briefly, each heavy chain typically comprises a heavy chain variable region (referred to herein as V H or VH) and a heavy chain constant region (C H The heavy chain constant region is typically composed of C H 1. C H 2, and C H The heavy chains are generally interconnected via disulfide bonds in the so-called "hinge region". Each light chain typically comprises a light chain variable region (herein referred to as V L or VL) and the light chain constant region (C L The light chain constant region is typically composed of one domain, C L A CL may be of the κ (kappa) or λ (lambda) isotype. The terms "constant domain" and "constant region" are used interchangeably herein. Immunoglobulins can be derived from any of the commonly known isotypes, including, but not limited to, IgA, secretory IgA, IgG, and IgM. IgG subclasses are also well known to those of skill in the art and include, but are not limited to, human IgG1, IgG2, IgG3, and IgG4. "Isotype" refers to the antibody class or subclass (e.g., IgM or IgG1) that is encoded by the heavy chain constant region genes.

[0016] The term "variable region" or "variable domain" refers to the domain of an antibody heavy or light chain that is involved in binding the antibody to an antigen. The variable regions of the heavy and light chains of a natural antibody (V H and V L) are further subdivided into regions of hypervariability (or hypervariable regions, which may be in the form of sequence and / or structurally defined loops), also called complementarity determining regions (CDRs), and may be interspersed with more conserved regions called framework regions (FRs). The terms "complementarity determining region" and "CDR", which are synonymous with "hypervariable region" or "HVR", are known in the art to refer to non-contiguous sequences of amino acids within an antibody variable region that confer antigen specificity and / or binding affinity. Generally, there are three CDRs in each heavy chain variable region (CDR-H1, CDR-H2, CDR-H3) and three CDRs in each light chain variable region (CDR-L1, CDR-L2, CDR-L3). The terms "framework region" and "FR" are known in the art to refer to the non-CDR portions of the heavy and light chain variable regions. Generally, there are four FRs in each full-length heavy chain variable region (FR-H1, FR-H2, FR-H3, and FR-H4), and four FRs in each full-length light chain variable region (FR-L1, FR-L2, FR-L3, and FR-L4). H and V L Within, the three CDRs and four FRs are typically arranged from amino terminus to carboxy terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4 (see also Chothia and Lesk J. Mot. Biol., 195, 901-917 (1987)).

[0017] The term "antibody" (Ab) in the context of the present invention refers to an immunoglobulin molecule, a fragment of an immunoglobulin molecule, or a derivative of either thereof, that has the ability to specifically bind to an antigen under typical physiological conditions with a half-life of significant duration, e.g., at least about 30 minutes, at least about 45 minutes, at least about 1 hour (h), at least about 2 hours, at least about 4 hours, at least about 8 hours, at least about 12 hours (h), about 24 hours or more, about 48 hours or more, about 3 days, 4 days, 5 days, 6 days, 7 days or more, etc., or any other relevant functionally defined period of time (e.g., a time sufficient to induce, promote, enhance, and / or modulate a physiological response associated with binding of the antibody to the antigen and / or a time sufficient for the antibody to recruit effector activity). The variable regions of the heavy and light chains of an immunoglobulin molecule contain the binding domains that interact with the antigen. The constant region of an antibody (Ab) may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g., effector cells) and components of the complement system, such as C1q, the first component of the classical pathway of complement activation. The antibody may also be a bispecific antibody, diabody, multispecific antibody, or similar molecule.

[0018] The term "monoclonal antibody" as used herein refers to a preparation of antibody molecules recombinantly produced with a single primary amino acid sequence and generated from a murine B-cell fusion. A monoclonal antibody composition exhibits a single binding specificity and affinity for a particular epitope. Thus, the term "human monoclonal antibody" refers to an antibody exhibiting a single binding specificity having variable and constant regions derived from human germline immunoglobulin sequences. Human monoclonal antibodies can be produced by hybridomas comprising B cells obtained from a transgenic or transchromosomal non-human animal, e.g., a transgenic mouse, whose genome comprises human heavy and light chain transgenes fused to an immortalized cell.

[0019] An "isolated antibody" refers to an antibody that is substantially free of other antibodies with different antigen specificities (e.g., an isolated antibody that specifically binds to αvβ6 is substantially free of antibodies that specifically bind to antigens other than αvβ6). However, an isolated antibody that specifically binds to αvβ6 may have cross-reactivity to other antigens, such as αvβ6 molecules from different species. Furthermore, an isolated antibody may be substantially free of other cellular material and / or chemicals. In one embodiment, an isolated antibody comprises an antibody conjugate bound to another agent (e.g., a small molecule drug). In some embodiments, an isolated anti-αvβ6 antibody comprises a conjugate of an anti-αvβ6 antibody with a small molecule drug (e.g., MMAE or MMAF).

[0020] A "human antibody" (HuMAb) refers to an antibody having a variable region in which both the FRs and CDRs are derived from human germline immunoglobulin sequences. Furthermore, if the antibody contains a constant region, the constant region is also derived from a human germline immunoglobulin sequence. The human antibodies of the present disclosure can include amino acid residues not encoded by human germline immunoglobulin sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo). However, the term "human antibody", as used herein, is not intended to include antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences. The terms "human antibody" and "fully human antibody" are used interchangeably.

[0021] The term "humanized antibody" as used herein refers to a genetically engineered non-human antibody comprising a human antibody constant domain and a non-human variable domain modified to contain a high level of sequence homology to the human variable domain. This can be achieved by grafting the six non-human antibody complementarity determining regions (CDRs), which together form the antigen binding site, onto a homologous human acceptor framework region (FR) (see WO 92 / 22653 and EP 0629240). Substitution of framework residues from the parent antibody (i.e., non-human antibody) to human framework regions (back mutations) may be required to fully reconstitute the binding affinity and specificity of the parent antibody. Structural homology modeling may help to identify amino acid residues in the framework regions that are important for the binding properties of the antibody. Thus, a humanized antibody may comprise primarily human framework regions, including non-human CDR sequences, optionally one or more amino acid back mutations to non-human amino acid sequences, and a fully human constant region. Optionally, further amino acid modifications, not necessarily back mutations, can be applied to obtain humanized antibodies with favorable properties, such as affinity and biochemical properties.

[0022] The term "chimeric antibody" as used herein refers to an antibody whose variable region is derived from a non-human species (e.g., from a rodent) and whose constant region is derived from a different species, e.g., from a human. Chimeric antibodies can be produced by antibody engineering. "Antibody engineering" is a term commonly used for different types of modification of antibodies and is a method well known to those skilled in the art. In particular, chimeric antibodies can be produced by using standard DNA techniques as described in Sambrook et al., 1989, Molecular Cloning: A laboratory Manual, New York: Cold Spring Harbor Laboratory Press, Chapter 15. In this way, chimeric antibodies can be genetically or enzymatically engineered recombinant antibodies. It is within the knowledge of a person skilled in the art to produce chimeric antibodies, and therefore the production of chimeric antibodies according to the present invention can be performed by methods other than those described herein. To reduce antibody immunogenicity, chimeric monoclonal antibodies have been developed for therapeutic applications. They can typically contain non-human (e.g., mouse) variable regions specific for the antigen of interest, and human constant antibody heavy and light chain domains. The term "variable region" or "variable domain" when used in the context of a chimeric antibody refers to the region comprising the CDR and framework regions of both the heavy and light immunoglobulin chains.

[0023] "Anti-antigen antibody" refers to an antibody that binds to an antigen. For example, an anti-αvβ6 antibody is an antibody that binds to the antigen αvβ6.

[0024] An "antigen-binding portion" or "antigen-binding fragment" of an antibody refers to one or more fragments of an antibody that retain the ability to specifically bind to the antigen bound by the whole antibody. Examples of antibody fragments (e.g., antigen-binding fragments) include, but are not limited to, Fv, Fab, Fab', Fab'-SH, F(ab')2; diabodies; linear antibodies; single-chain antibody molecules (e.g., scFv); and multispecific antibodies formed from antibody fragments. Papain digestion of an antibody produces two identical antigen-binding fragments called "Fab" fragments, each with a single antigen-binding site, and a residual "Fc" fragment, the name reflecting its ability to crystallize easily. Pepsin treatment produces an F(ab')2 fragment that has two antigen-binding sites and is also capable of cross-linking antigens.

[0025] "Percent sequence identity" with respect to a reference polypeptide sequence is defined as the percentage of amino acid residues in a candidate sequence that are identical to those in the reference polypeptide sequence, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity, without considering any conservative substitutions as part of the sequence identity. Alignment for purposes of determining percent amino acid sequence identity can be accomplished in a variety of ways that are within the skill of the art, for example, using publicly available computer software, such as BLAST, BLAST-2, SnapGene Align or ClustalW BioEdit software. Those skilled in the art can determine appropriate parameters for aligning sequences, including any algorithms necessary to achieve maximum alignment over the entire length of the sequences being compared. For example, the percent sequence identity of a given amino acid sequence A to, with, or against a given amino acid sequence B (alternatively, it can be expressed as a given amino acid sequence A having or containing a certain percent sequence identity to, with, or against a given amino acid sequence B) can be expressed as follows: 100 x fraction X / Y where X is the number of amino acid residues scored as perfect identity to the sequence in the alignment of A and B by the program, and Y is the total number of amino acid residues in B. It is understood that if the length of amino acid sequence A is not equal to the length of amino acid sequence B, then the % sequence identity of A to B is not equal to the % sequence identity of B to A.

[0026] As used herein, the terms "binding," "bind," or "specifically bind" in the context of binding of an antibody to a given antigen typically refer to a binding activity of about 10 to about 20% of the antibody as determined, for example, by BioLayer Interferometry (BLI) technology on an Octet HTX instrument using the antibody as the ligand and the antigen as the analyte. -6 M or less, e.g. 10 -7 M or less, for example, about 10 -8 M or less, for example, about 10 -9 M or less, about 10 -10 M or less, or even about 10 -11 M or less, where the antibody has a KD of less than or equal to M for binding to a nonspecific antigen other than the given antigen or a closely related antigen (e.g., BSA, casein). D at least 10 times lower, such as at least 100 times lower, such as at least 1,000 times lower, such as at least 10,000 times lower, such as at least 100,000 times lower D binds to a given antigen with an affinity corresponding to the K D The lower the amount of antibody, the higher the K D Therefore, the K D If the K of binding to the antigen is very low, D is the K for nonspecific antigen binding. D The amount lower may be at least 10,000 fold (ie, the antibody is highly specific).

[0027] The term “K D " (M) as used herein refers to the dissociation equilibrium constant of a particular antibody-antigen interaction. As used herein, affinity and K Dis inversely related, i.e., higher affinity corresponds to lower K D A lower affinity is intended to refer to a higher K D is intended to refer to.

[0028] The term "ADC" refers to an antibody drug conjugate, which in the context of the present invention refers to an anti-αvβ6 antibody linked to a drug moiety (e.g., MMAE or MMAF), as described in this application.

[0029] The abbreviations "vc" and "val-cit" refer to the dipeptide valine-citrulline.

[0030] The abbreviation "VKG" refers to the tripeptide linker valine-lysine-glycine.

[0031] The abbreviation "PAB" stands for self-immolative spacer: [ka]

[0032] The abbreviation "MC" stands for the stretcher maleimidocaproyl: [ka]

[0033] The abbreviation "MP" stands for the stretcher maleimidopropionyl: [ka]

[0034] As used herein, a "PEG unit" is an organic moiety composed of repeating ethyleneoxy subunits (PEGs or PEG subunits), which may be polydisperse, monodisperse, or discrete (i.e., having a discrete number of ethyleneoxy subunits). Polydisperse PEGs are a heterogeneous mixture of sizes and molecular weights, while monodisperse PEGs are typically purified from a heterogeneous mixture, thus providing a single chain length and molecular weight. A preferred PEG unit is composed of discrete PEGs, which are compounds that are synthesized stepwise, rather than via a polymerization process. Discrete PEGs provide single molecules with defined and specific chain lengths.

[0035] The PEG units provided herein comprise one or more polyethylene glycol chains, each composed of one or more ethyleneoxy subunits, covalently linked to one another. The polyethylene glycol chains may be linked to one another, for example, in a linear, branched, or star configuration. Typically, at least one of the polyethylene glycol chains prior to incorporation into a camptothecin conjugate is derivatized at one end with an alkyl moiety substituted with an electrophilic group for covalently bonding to the carbamate nitrogen of the methylene carbamate unit (i.e., represents an example of R). Typically, the terminal ethyleneoxy subunit in each polyethylene glycol chain that is not involved in covalent bonding to the remainder of the linker unit is modified with a PEG capping unit, typically an optionally substituted alkyl such as -CH3, CH2CH3, or CH2CH2CO2H. A preferred PEG unit has a single polyethylene glycol chain with 2-24 -CH2CH2O- subunits covalently linked in series and terminated at one end with a PEG capping unit.

[0036] "Cancer" refers to a broad group of different diseases characterized by the uncontrolled growth of abnormal cells in the body. "Cancer" or "cancerous tissue" can include tumors. Uncontrolled cell division and growth leads to the formation of malignant tumors that can invade adjacent tissues and also metastasize to distant sites in the body through the lymphatic system or bloodstream. After metastasis, the distant tumor is said to "originate" from the pre-metastatic tumor.

[0037] The term "antibody-dependent cellular cytotoxicity" or ADCC is a mechanism for inducing cell death that depends on the interaction of antibody-coated target cells with immune cells (also called effector cells) that possess lytic activity. Such effector cells include natural killer cells, monocytes / macrophages, and neutrophils. Effector cells bind via their antigen-binding site to the Fc effector domain(s) of Ig bound to the target cell. Killing of the antibody-coated target cell occurs as a result of effector cell activity.

[0038] The term "antibody-dependent cellular phagocytosis" or ADCP refers to the process by which antibody-coated cells are internalized in whole or in part by phagocytic immune cells (e.g., macrophages, neutrophils, and dendritic cells) that bind to the Fc effector domain(s) of Ig.

[0039] The term "complement-dependent cytotoxicity" or CDC refers to a mechanism for inducing cell death in which the Fc effector domain(s) of a target-bound antibody activate a series of enzymatic reactions resulting in the formation of holes in the target cell membrane. Typically, antigen-antibody complexes, e.g., on antibody-coated target cells, bind and activate complement component C1q, which in turn activates the complement cascade leading to target cell death. Complement activation can also result in the deposition of complement components on the target cell surface, facilitating ADCC through binding of complement receptors (e.g., CR3) on leukocytes.

[0040] "Cytostatic effect" refers to the inhibition of cell proliferation. "Cytostatic agent" refers to an agent that has a cytostatic effect on cells, thereby inhibiting the proliferation and / or expansion of a specific subset of cells. The cytostatic agent may be conjugated to an antibody or administered in combination with an antibody.

[0041] "Treatment" or "therapy" of a subject means any type of intervention or process performed on a subject, or the administration of an active agent to a subject, for the purpose of reversing, alleviating, ameliorating, inhibiting, delaying, or preventing the onset, progression, development, severity, or recurrence of a symptom, complication, condition, or biochemical manifestation associated with a disease. In some embodiments, the disease is cancer.

[0042] A "subject" includes any human or non-human animal. The term "non-human animal" includes, but is not limited to, vertebrates such as non-human primates, sheep, dogs, and rodents (e.g., mice, rats, and guinea pigs). In some embodiments, the subject is a human. The terms "subject" and "patient" and "individual" are used interchangeably herein.

[0043] An "effective amount" or "therapeutically effective amount" or "therapeutically effective dosage" of a drug or therapeutic agent is that amount of the drug that, when used alone or in combination with another therapeutic agent, protects a subject from developing a disease or promotes regression of the disease as evidenced by a decrease in the severity of disease symptoms, an increase in the frequency and duration of symptom-free periods of the disease, or prevention of dysfunction or disability due to illness of the disease. The ability of a therapeutic agent to promote disease regression can be assessed using a variety of methods known to the skilled practitioner, for example, by assaying the activity of the agent in human subjects in clinical trials, in animal model systems that are predictive of efficacy in humans, or in in vitro assays.

[0044] As an example for treating tumors, a therapeutically effective amount of an anti-cancer agent inhibits cell proliferation or tumor growth by at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, or at least about 80%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% in a treated subject (e.g., one or more treated subjects) compared to an untreated subject (e.g., one or more untreated subjects). In some embodiments, a therapeutically effective amount of an anti-cancer agent inhibits cell proliferation or tumor growth by 100% in a treated subject (e.g., one or more treated subjects) relative to an untreated subject (e.g., one or more untreated subjects).

[0045] In other embodiments of the present disclosure, tumor regression can be observed and continued for a period of at least about 20 days, at least about 30 days, at least about 40 days, at least about 50 days, or at least about 60 days.

[0046] The therapeutically effective amount of a drug (e.g., an anti-αvβ6 antibody drug conjugate) includes a "prophylactically effective amount," which is any amount of drug that inhibits the development or recurrence of cancer when administered alone or in combination with an anti-cancer drug to a subject at risk of developing cancer (e.g., a subject with a pre-malignant condition) or a subject at risk of suffering from cancer recurrence. In some embodiments, a prophylactically effective amount completely prevents the development or recurrence of cancer. "Inhibiting" the development or recurrence of cancer means either reducing the likelihood of the development or recurrence of cancer, or completely preventing the development or recurrence of cancer.

[0047] As used herein, a "subtherapeutic dose" refers to a dose of a therapeutic compound (e.g., an anti-αvβ6 antibody drug conjugate) that is lower than the usual or typical dose when the therapeutic compound is administered alone for the treatment of a proliferative disease (e.g., cancer).

[0048] "Immune-related response pattern" refers to a clinical response pattern often observed in cancer patients treated with immunotherapeutic agents that induce cancer-specific immune responses or modify natural immune processes to produce antitumor effects. This response pattern is characterized by an initial increase in tumor burden or the appearance of new lesions, which in the evaluation of conventional chemotherapeutic agents would be classified as disease progression and would be synonymous with drug failure, followed by a beneficial therapeutic effect. Thus, proper evaluation of immunotherapeutic agents may require longitudinal monitoring of the effects of these agents on the target disease.

[0049] By way of example, an "anti-cancer drug" promotes the regression of cancer in a subject. In some embodiments, a therapeutically effective amount of the drug promotes the regression of cancer to the point where the cancer disappears. "Promoting the regression of cancer" means that the administration of an effective amount of the drug, alone or in combination with an anti-cancer drug, results in a reduction in tumor growth or size, tumor necrosis, a decrease in the severity of at least one disease symptom, an increase in the frequency and duration of disease-free periods, or prevention of dysfunction or disability due to disease affliction. In addition, the terms "effective" and "efficacy" in relation to treatment include both pharmacological efficacy and physiological safety. Pharmacological efficacy refers to the ability of a drug to promote the regression of cancer in a patient. Physiological safety refers to the level of toxicity or other adverse physiological effects (adverse effects) at the cellular, organ, and / or organismal levels resulting from the administration of the drug.

[0050] "Sustained response" refers to a sustained effect on the reduction of tumor growth after treatment has stopped. For example, the tumor size may remain the same or smaller compared to the size at the beginning of the dosing phase. In some embodiments, the sustained response has a duration at least as long as the duration of treatment, or at least 1.5, 2.0, 2.5, or 3.0 times as long as the duration of treatment.

[0051] As used herein, "complete response" or "CR" means disappearance of all target lesions; "partial response" or "PR" means at least a 30% reduction in the sum of longest diameters (SLD) of target lesions based on baseline SLD; and "stable disease" or "SD" means neither sufficient shrinkage of target lesions to qualify for PR nor sufficient increase, based on the smallest SLD since treatment initiation, to qualify for PD.

[0052] As used herein, "progression free survival" or "PFS" refers to the length of time during and after treatment during which the disease being treated (e.g., cancer) does not worsen. Progression free survival can include periods during which a patient experiences a complete or partial response, as well as periods during which a patient experiences stable disease.

[0053] As used herein, "overall response rate" or "ORR" means the sum of the complete response (CR) rate and the partial response (PR) rate.

[0054] As used herein, "overall survival" or "OS" refers to the percentage of individuals in a group who are likely to be alive after a particular period of time.

[0055] The phrase "pharmacologically acceptable" indicates that a substance or composition must be chemically and / or toxicologically compatible with the other ingredients that make up the formulation and / or with the mammal being treated therewith.

[0056] The phrase "pharmaceutically acceptable salt," as used herein, means a pharmaceutically acceptable organic or inorganic salt of a compound of the present invention. Exemplary salts include, but are not limited to, sulfate, citrate, acetate, oxalate, chloride, bromide, iodide, nitrate, bisulfate, phosphate, acid phosphate, isonicotinate, lactate, salicylate, acid citrate, tartrate, oleate, tannate, pantothenate, bitartrate, ascorbate, succinate, maleate, gentisinate, fumarate, gluconate, glucuronate, saccharate, formate, benzoate, glutamate, methanesulfonate "mesylate", ethanesulfonate, benzenesulfonate, p-toluenesulfonate, pamoate (i.e., 4,4'-methylene-bis-(2-hydroxy-3-naphthoate)), alkali metal (e.g., sodium and potassium) salts, alkaline earth metal (e.g., magnesium) salts, and ammonium salts. Pharmaceutically acceptable salts can also include those that contain another molecule, such as acetate, succinate or other counter ions. Counter ions can be any organic or inorganic moiety that stabilizes the charge on the parent compound. In addition, pharmaceutically acceptable salts can have more than one charged atom in their structure. When multiple charged atoms are part of the pharmaceutically acceptable salt, it can have multiple counter ions. Thus, pharmaceutically acceptable salts can have one or more charged atoms and / or one or more counter ions.

[0057] "Administering" or "administration" refers to the physical introduction of a therapeutic agent into a subject using any of a variety of methods and delivery systems known to those skilled in the art. Exemplary administration routes for anti-αvβ6 antibody drug conjugates include intravenous, intramuscular, subcutaneous, intraperitoneal, spinal or other parenteral administration routes, such as by injection or infusion (preferably intravenous). The phrase "parenteral administration" as used herein means a mode of administration other than enteral and topical administration, usually by injection, including, but not limited to, intravenous, intramuscular, intraarterial, subarachnoid, intralymphatic, intralesional, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intrathecal, epidural and intrasternal injection and infusion, and in vivo electroporation. The therapeutic agent can be administered via a non-parenteral route or orally. Other non-parenteral routes include topical, epidermal or mucosal routes of administration, such as intranasal, vaginal, rectal, sublingual or topical routes of administration. Administration can also be, for example, once, multiple times, and / or over one or more extended periods of time.

[0058] The term "baseline" or "baseline value" as used interchangeably herein can refer to a measurement or characterization of a symptom (symptom) before administration of a treatment (e.g., an anti-αvβ6 antibody drug conjugate as described herein) or at the start of administration of a treatment. The baseline value can be compared to a reference value to determine the alleviation or improvement of a symptom of an αvβ6-related disease (e.g., cancer) as contemplated herein. The term "reference" or "reference value" as used interchangeably herein can refer to a measurement or characterization of a symptom after administration of a treatment (e.g., an anti-αvβ6 antibody drug conjugate as described). The reference value can be measured one or more times during a dosing regimen or treatment cycle or at the completion of a dosing regimen or treatment cycle. The "reference value" can be an absolute value, a relative value, a value with upper and / or lower limits, a range of values, an average value, a median value, a mean value, or a value compared to a baseline value.

[0059] Similarly, a "baseline value" can be an absolute value, a relative value, a value with upper and / or lower limits, a range of values, an average value, a median value, a mean value, or a value compared to a reference value. Reference and / or baseline values ​​can be obtained from one individual, from two different individuals, or from a group of individuals (e.g., a group of 2, 3, 4, 5 or more individuals).

[0060] The term "monotherapy" as used herein means that the anti-αvβ6 antibody drug conjugate is the only anti-cancer drug administered to the subject during the treatment cycle.However, other therapeutic agents can be administered to the subject.For example, anti-inflammatory agents or other agents administered to the subject with cancer to treat symptoms related to cancer, not the underlying cancer itself, such as inflammation, pain, weight loss, general fatigue, etc., can be administered during monotherapy.

[0061] An "adverse event" (AE), as used herein, is any unfavourable and generally unintended or undesirable sign (including abnormal laboratory findings), symptom, or disease associated with the use of a medical treatment. A medical treatment may have one or more associated AEs, each of which may have the same or different levels of severity. Reference to a method that can "modify an adverse event" refers to a treatment regimen that reduces the incidence and / or severity of one or more AEs associated with the use of a different treatment regimen.

[0062] As used herein, a "serious adverse event" or "SAE" refers to an adverse event that meets one of the following criteria: -Fatal or life-threatening ("life-threatening" as used in the definition of serious adverse events refers to an event in which the patient was at risk of death at the time of the event, not a more severe event that would have hypothetically resulted in death). -Causing permanent or significant disability / incapacity. -Congenital anomalies / birth defects. - Is medically significant, i.e., is defined as an event that may endanger the patient or require medical or surgical intervention to prevent one of the outcomes listed above. The determination of whether an AE is "medically significant" requires the use of medical and scientific judgment. - Requiring hospitalization or extension of an existing hospitalization, except for: 1) routine treatment or monitoring of an underlying disease without a worsening of the condition; 2) elective or pre-planned treatment for a pre-existing condition that is unrelated to the indication under study and that has not worsened since signing the informed consent; 3) social reasons and respite care when the patient's general condition has not worsened.

[0063] The use of the alternative (e.g., "or") should be understood to mean either one, both, or any combination of the alternatives. As used herein, the indefinite article "a" or "an" should be understood to mean "one or more" of any described or listed members.

[0064] The term "about" or "essentially consisting of" means a value or composition that is within an acceptable error range for a particular value or composition as determined by one of ordinary skill in the art, which will depend on how the value or composition is measured or determined, i.e., the limitations of the measurement system. For example, "about" or "essentially consisting of" can mean within one or more standard deviations, according to practice in the art. Alternatively, "about" or "essentially consisting of" can mean a range of up to 20%. Moreover, particularly with respect to biological systems or processes, the term can mean up to an order of magnitude or up to 5 times the value. When a particular value or composition is given in this application and claims, unless otherwise stated, the meaning of "about" or "essentially consisting of" should be presumed to be within an acceptable error range for that particular value or composition.

[0065] Reference herein to "about" a value or parameter includes (and describes) embodiments that are directed to that value or parameter per se. For example, a description that references "about X" includes and describes "X."

[0066] As described herein, any concentration range, percentage range, ratio range, or integer range, unless otherwise indicated, should be understood to include any integer value within the stated range, and, where appropriate, fractions thereof (such as tenths and hundredths of integers).

[0067] Various aspects of the disclosure are described in further detail in the subsections below.

[0068] II. General The present invention provides an antibody that specifically binds to αvβ6. The present invention is based in part on the discovery that antibody drug conjugates that target αvβ6, such as vcMMAE antibody drug conjugates, are particularly effective in killing αvβ6+ expressing cells. αvβ6 has been shown to be expressed in a variety of cancers, including non-small cell lung cancer (NSCLC) (squamous cell carcinoma and adenocarcinoma), head and neck cancer (including head and neck squamous cell carcinoma), esophageal cancer, breast cancer (including breast invasive carcinoma), ovarian cancer, bladder cancer (including urothelial carcinoma), skin cancer (squamous cell carcinoma, i.e., SCC), kidney cancer (including clear cell renal cell carcinoma, papillary renal cell carcinoma, and chromophobe renal cell carcinoma), cervical cancer, gastric cancer, prostate cancer (including prostate adenocarcinoma), endometrial cancer (including uterine carcinosarcoma and endometrial carcinoma), rectal adenocarcinoma, thyroid cancer, colon adenocarcinoma, gastric adenocarcinoma, and pancreatic cancer (including pancreatic adenocarcinoma).

[0069] III. Target molecule Unless otherwise specified, αvβ6 refers to human αvβ6. An exemplary β6 human sequence is assigned GenBank accession number AAA36122. An exemplary αv human sequence is assigned NCBI NP_002201.1.

[0070] IV. Antibodies of the Invention The present invention provides murine 2A2 antibodies, as well as chimeric, humanized and human 2A2 antibodies.

[0071] The affinity of the antibodies of the present invention (e.g., chimeric, humanized, and human forms of the mouse 2A2 antibody) for human αvβ6 is preferably equal to, greater than, or within 10-fold, 5-fold, or 2-fold weaker than the affinity of the mouse 2A2 antibody for human αvβ6. One method for measuring the affinity of an antibody for a target antigen is to determine the apparent dissociation constant of the antibody. The present invention also encompasses antibodies (e.g., chimeric, humanized, and human forms of the mouse 2A2 antibody) that have an apparent dissociation constant essentially the same as that of the mouse 2A2 (i.e., within experimental error), as well as antibodies that have a dissociation constant lower or higher than that of the mouse antibody 2A2 for human αvβ6. The chimeric, humanized, and human 2A2 antibodies, like the mouse 2A2 antibody, specifically bind to human αvβ6 in its native form and / or recombinantly expressed from CHO cells. Typically, chimeric, humanized and human 2A2 anti-αvβ6 antibodies compete with mouse 2A2 for binding to human αvβ6.

[0072] Preferred antibodies of the present invention are those that inhibit cancer (e.g., cell proliferation, metastasis and / or lethality to the organism) as shown in cancer cells grown in culture, in animal models or in clinical trials. Animal models can be formed by implanting human tumor cell lines expressing αvβ6 into appropriate immunodeficient rodent strains, such as thymic nude mice or SCID mice. These tumor cell lines can be established in immunodeficient rodent hosts as solid tumors by subcutaneous injection or as disseminated tumors by intravenous injection. Once established in the host, these tumor models can be applied to evaluate the therapeutic efficacy of anti-αvβ6 antibodies or their conjugated forms, as described in the Examples.

[0073] In general, the anti-αvβ6 antibody and / or anti-αvβ6 antibody drug conjugate of the present disclosure binds to αvβ6, e.g., human αvβ6, and exerts cytostatic and cytotoxic effects on malignant cells (e.g., cancer cells). The anti-αvβ6 antibody of the present disclosure is preferably monoclonal, and may be a multispecific, human, humanized or chimeric antibody, a single chain antibody, a Fab fragment, a F(ab') fragment, a fragment generated by a Fab expression library, and an αvβ6-binding fragment of any of the above. In some embodiments, the anti-αvβ6 antibody of the present disclosure specifically binds to αvβ6. The immunoglobulin molecule of the present disclosure may be of any type (e.g., IgG, IgE, IgM, IgD, IgA, and IgY), class (e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2), or subclass of immunoglobulin molecule.

[0074] In certain embodiments of the disclosure, the anti-αvβ6 antibody is an antigen-binding fragment (e.g., a human antigen-binding fragment) as described herein, including, but not limited to, Fab, Fab' and F(ab')2, Fd, single chain Fv (scFv), single chain antibodies, disulfide-linked Fv (sdFv), and VFv. L Or V H Antigen-binding fragments include fragments that contain any of the variable regions, including single chain antibodies, either alone or in combination with the whole or a portion of the following: hinge region, CH1, CH2, CH3, and CL domains. Antigen-binding fragments that contain any combination of the variable region and the hinge region, CH1, CH2, CH3, and CL domains are also included in the present disclosure. In some embodiments, the anti-αvβ6 antibody or antigen-binding fragment thereof is human, murine (e.g., mouse and rat), donkey, sheep, rabbit, goat, guinea pig, camel, horse, or chicken.

[0075] The anti-αvβ6 antibodies of the present disclosure may be monospecific, bispecific, trispecific, or of greater multispecificity. Multispecific antibodies may be specific for different epitopes of αvβ6, or may be specific for both αvβ6 and a heterologous protein.

[0076] The anti-αvβ6 antibodies of the present disclosure can be described or specified in terms of the particular CDRs they contain.The precise amino acid sequence boundaries of a given CDR or FR can be determined by Kabat et al. (1991), "Sequences of Proteins of Immunological Interest," 5th Ed. Public Health Service, National Institutes of Health, Bethesda, MD ("Kabat" numbering scheme); Al-Lazikani et al., (1997) JMB 273,927-948 ("Chothia" numbering scheme); MacCallum et al., J. Mol. Biol. 262:732-745 (1996), "Antibody-antigen interactions: Contact analysis and binding site topography," J. Mol. Biol. 262, 732-745 ("Contact" numbering scheme); Lefranc MP et al., "IMGT unique numbering for immunoglobulin and T cell receptor variable domains and Ig superfamily V-like domains," Dev Comp Immunol, 2003 Jan;27(1):55-77 ("IMGT" numbering scheme); Honegger A and Pluckthun A, "Yet another numbering scheme for immunoglobulin variable domains: an automatic modeling and analysis tool," J Mol Biol, 2001 Jun 8;309(3):657-70 ("Aho" numbering scheme); and Martin et al., "Modeling antibody hypervariable loops: a combined algorithm," PNAS, 1989, 86(23):9268-9272 ("AbM" numbering scheme).The boundaries of a given CDR may vary depending on the scheme used for identification. In some embodiments, the "CDR" or "complementarity determining region" of a given antibody or region thereof (e.g., its variable region), or each designated CDR (e.g., CDR-H1, CDR-H2, CDR-H3) should be understood to encompass the CDRs defined (or specified) by any of the aforementioned schemes. For example, a particular CDR (e.g., CDR-H3) may be a CDR that is specific to a given V. H Area or V L When a region is described as including the amino acid sequence of a corresponding CDR in its amino acid sequence, it is understood that such CDR has the sequence of the corresponding CDR (e.g., CDR-H3) in the variable region as defined by any of the above schemes. Schemes for the identification of a particular CDR or CDRs may be specified, such as CDRs defined by the Kabat, Chothia, AbM or IMGT methods.

[0077] In one embodiment, the CDR sequences of the anti-αvβ6 antibodies and anti-αvβ6 antibody drug conjugates described herein follow the Kabat numbering scheme. In another embodiment, the CDR sequences of the anti-αvβ6 antibodies and anti-αvβ6 antibody drug conjugates described herein follow the IMGT numbering scheme.

[0078] In one aspect, provided herein is an anti-αvβ6 antibody comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises (i) CDR-H1 comprising the amino acid sequence of SEQ ID NO: 31, (ii) CDR-H2 comprising the amino acid sequence of SEQ ID NO: 32, and (iii) CDR-H3 comprising the amino acid sequence of SEQ ID NO: 33; and / or the light chain variable region comprises (i) CDR-L1 comprising the amino acid sequence of SEQ ID NO: 37, (ii) CDR-L2 comprising the amino acid sequence of SEQ ID NO: 42, and (iii) CDR-L3 comprising the amino acid sequence of SEQ ID NO: 39, wherein the CDRs of the anti-αvβ6 antibody are defined according to the Kabat numbering scheme. In other embodiments, the CDR-L1 comprises the amino acid sequence of SEQ ID NO: 40. In other embodiments, the CDR-L2 comprises the amino acid sequence of SEQ ID NO: 38 or 41.

[0079] In one aspect, provided herein is an anti-αvβ6 antibody comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises (i) CDR-H1 comprising the amino acid sequence of SEQ ID NO: 34, (ii) CDR-H2 comprising the amino acid sequence of SEQ ID NO: 35, and (iii) CDR-H3 comprising the amino acid sequence of SEQ ID NO: 36; and / or the light chain variable region comprises (i) CDR-L1 comprising the amino acid sequence of SEQ ID NO: 43, (ii) CDR-L2 comprising the amino acid sequence of SEQ ID NO: 44, and (iii) CDR-L3 comprising the amino acid sequence of SEQ ID NO: 45, wherein the CDRs of the anti-αvβ6 antibody are defined according to the IMGT numbering scheme.

[0080] The anti-αvβ6 antibodies described herein can comprise any suitable framework variable domain sequence, provided that the antibody retains the ability to bind to αvβ6 (e.g., human αvβ6). In some embodiments of the anti-αvβ6 antibodies described herein, the heavy and light chain variable domains comprise the amino acid sequences of SEQ ID NOs: 6 and 17, respectively. In some embodiments of the anti-αvβ6 antibodies described herein, the heavy and light chain variable domains comprise the amino acid sequences of SEQ ID NOs: 21 and 29, respectively.

[0081] In some embodiments, provided herein are anti-αvβ6 antibodies and / or anti-αvβ6 antibody drug conjugates comprising a heavy chain variable domain comprising an amino acid sequence having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to the amino acid sequence of SEQ ID NO: 6. In certain embodiments, the heavy chain variable domain comprising an amino acid sequence having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to the amino acid sequence of SEQ ID NO: 6 comprises substitutions (e.g., conservative substitutions), insertions, or deletions compared to the reference sequence and retains the ability to bind to αvβ6 (e.g., human αvβ6). In certain embodiments, a total of 1-10 amino acids are substituted, inserted and / or deleted in SEQ ID NO:6. In other embodiments, a total of 3-10 amino acids are substituted, inserted and / or deleted in SEQ ID NO:6. In certain embodiments, the substitutions, insertions or deletions (e.g., 1, 2, 3, 4, or 5 amino acids) occur in the inner regions of the CDRs. In certain embodiments, the substitutions, insertions or deletions (e.g., 1, 2, 3, 4, or 5 amino acids) occur in the outer regions of the CDRs (i.e., in the FRs). In some embodiments, the anti-αvβ6 antibody comprises a heavy chain variable domain sequence of SEQ ID NO:6 (including post-translationally modified versions of that sequence).

[0082] In some embodiments, provided herein are anti-αvβ6 antibodies and / or anti-αvβ6 antibody drug conjugates comprising a light chain variable domain comprising an amino acid sequence having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to the amino acid sequence of SEQ ID NO: 17. In certain embodiments, the light chain variable domain comprising an amino acid sequence having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to the amino acid sequence of SEQ ID NO: 17 comprises substitutions (e.g., conservative substitutions), insertions, or deletions compared to the reference sequence and retains the ability to bind to αvβ6 (e.g., human αvβ6). In certain embodiments, a total of 1-10 amino acids are substituted, inserted and / or deleted in SEQ ID NO: 17. In other embodiments, a total of 1-2 amino acids are substituted, inserted and / or deleted in SEQ ID NO: 17. In certain embodiments, the substitutions, insertions or deletions (e.g., 1, 2, 3, 4, or 5 amino acids) occur in the regions outside the CDRs (i.e., in the FRs). In some embodiments, the anti-αvβ6 antibody comprises the light chain variable domain sequence of SEQ ID NO: 17 (including post-translationally modified versions of that sequence).

[0083] There are five classes of immunoglobulins: IgA, IgD, IgE, IgG, and IgM, with heavy chains designated α, δ, ε, γ, and μ, respectively. The γ and α classes are further divided into subclasses, for example, humans express the following subclasses: IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2. IgG1 antibodies can exist in multiple polymorphic variants called allotypes (reviewed in Jefferis and Lefranc 2009. mAbs, Vol 1, Issue 4, 1-7), any of which are suitable for use in some embodiments herein. Common allotypic variants in the human population are those designated by the letters a, f, n, z, or combinations thereof. In any of the embodiments herein, the antibody can comprise a heavy chain Fc region that comprises a human IgG Fc region. In a further embodiment, the human IgG Fc comprises a human IgG1.

[0084] The antibodies of the present invention also include derivatives that are modified, i.e., modified by the covalent attachment of any type of molecule to the antibody such that the covalent attachment does not prevent the antibody from binding to αvβ6 or from exerting a cytostatic or cytotoxic effect on HD cells. For example, but not limited to, antibody derivatives include antibodies that are modified by, for example, glycosylation, acetylation, PEGylation, phosphorylation, amidation, derivatization with known protecting / blocking groups, proteolytic cleavage, linkage to cellular ligands or other proteins, etc. Any of a number of chemical modifications can be made by known techniques, including, but not limited to, specific chemical cleavage, acetylation, formylation, metabolic synthesis of tunicamycin, etc. In addition, the derivatives can contain one or more non-classical amino acids.

[0085] Humanized antibodies Humanized antibodies are genetically engineered antibodies in which CDRs from a non-human "donor" antibody are grafted onto human "acceptor" antibody sequences (see, e.g., Queen, US 5,530,101 and 5,585,089; Winter, US 5,225,539; Carter, US 6,407,213; Adair, US 5,859,205; and Foote, US 6,881,557). The acceptor antibody sequences can be, for example, mature human antibody sequences, composites of such sequences, consensus sequences of human antibody sequences, or germline region sequences. Preferred acceptor sequences for the heavy chain are germline V H exon IGHV1-46 and J exon (J H For the light chain, the preferred acceptor sequence is exon IGKV1D-33 and for the J exon it is IGKJ2. Another preferred acceptor sequence for the heavy chain is the J exon (J H), IGHJ1, IGHJ2, IGHJ3, IGHJ5 or IGHJ6. Another preferred acceptor sequence for the light chain includes the J exon IGKJ1, IGKJ3, IGKJ4 or IGKJ5. Thus, a humanized antibody is an antibody that is derived entirely or substantially from a donor antibody and variable region framework sequences and constant regions, and has some or all of its CDRs, if present, derived entirely or substantially from human antibody sequences. Similarly, a humanized heavy chain is derived entirely or substantially from a donor antibody heavy chain and heavy chain variable region framework sequences and heavy chain constant regions, and has at least one, two and usually all three CDRs, if present, derived substantially from human heavy chain variable region framework and constant region sequences. Similarly, a humanized light chain is derived entirely or substantially from a donor antibody light chain and light chain variable region framework sequences and light chain constant regions, and has at least one, two and usually all three CDRs, if present, derived substantially from human light chain variable region framework and constant region sequences. Other than nanobodies and dAbs, humanized antibodies comprise a humanized heavy chain and a humanized light chain. The CDRs in a humanized antibody are substantially derived from the corresponding CDRs in a non-human antibody when at least 60%, 85%, 90%, 95%, or 100% of the corresponding residues (as defined by Kabat) are identical between the respective CDRs. The variable region framework sequences of the antibody chains or the constant regions of the antibody chains are substantially derived from human variable region framework sequences or human constant regions, respectively, when at least 85%, 90%, 95%, or 100% of the corresponding residues as defined by Kabat are identical.

[0086] Humanized antibodies often incorporate all six CDRs (preferably as defined by Kabat) from a murine antibody, although they can be made with fewer than all of the CDRs (e.g., at least three, four, or five) from the murine antibody (e.g., Pascalis et al., J. Immunol. 169:3076, 2002; Vajdos et al., Journal of Molecular Biology, 320: 415-428, 2002; Iwahashi et al., Mol. Immunol. 36:1079-1091, 1999; Tamura et al, Journal of Immunology, 164:1432-1441, 2000).

[0087] Certain amino acids from the human variable region framework residues may be selected for substitution based on their possible effects on CDR conformation and / or binding to antigen, the study of such possible effects being by modeling, by examination of the properties of the amino acid at a particular position, or by empirical observation of the effects of substitution or mutagenesis of a particular amino acid.

[0088] For example, when an amino acid differs between the murine variable region framework residue and a selected human variable region framework residue, the human framework amino acid can be replaced with the equivalent framework amino acid from the murine antibody if the amino acid is reasonably predicted to be: (1) Direct, non-covalent binding to antigens; (2) adjacent to the CDR region, (3) otherwise interacts with the CDR region (e.g., is within about 6 Å of the CDR region), or (4) mediates interactions between heavy and light chains.

[0089] Although the 2A2 antibody was identified as a murine antibody, the present application also encompasses human 2A2 antibodies. The term "human 2A2 antibody" refers to an antibody derived from a human immunoglobulin gene sequence, having substantially identical CDRs to those of the murine 2A2 antibody, and exhibiting similar properties, i.e., binding specificity for αvβ6. In some aspects, the human 2A2 antibody comprises a heavy chain variable region substantially identical to the heavy chain variable region described herein, and / or a light chain variable region substantially identical to the light chain variable region described herein. In some embodiments, the 2A2 antibody of the present invention is not a human antibody, e.g., the 2A2 antibody of the present invention is a murine antibody, a chimeric antibody, or a humanized antibody.

[0090] One aspect of the invention provides a humanized form of murine antibody 2A2. One such humanized variant of murine antibody 2A2 is referred to as HCLG. HCLG comprises a mature heavy chain variable region comprising the amino acid sequence of SEQ ID NO:6 and a mature light chain variable region comprising the amino acid sequence of SEQ ID NO:17. Humanized antibodies of the invention include variants of HCLG humanized antibodies in which the humanized heavy chain mature variable region exhibits at least 90%, 95% or 99% sequence identity to SEQ ID NO:6 and the humanized light chain mature variable region exhibits at least 90%, 95% or 99% sequence identity to SEQ ID NO:17. Preferably, in such antibodies, some or all of the back mutations of HCLG are retained. That is, at least 1, 2, 3, 4, 5, 6, 7, 8 or preferably all 9 of positions H2, H28, H48, H67, H69, H71, H73, H78, and H93 of the heavy chain are occupied by F, S, I, A, L, V, K, A, and T, respectively. Similarly, position L69 is preferably occupied by R and position L71 is preferably occupied by Y. The CDR regions can be defined by any conventional definition (e.g., Chothia), but are preferably as defined by Kabat or IMGT. In one embodiment, the humanized antibody comprises a heavy chain comprising the three CDRs of SEQ ID NO:6 and a variable region framework having at least 95% identity to the variable region framework of SEQ ID NO:6. In another embodiment, the humanized antibody comprises a light chain comprising the three CDRs of SEQ ID NO:17 and a variable region framework having at least 95% identity to the variable region framework of SEQ ID NO:17. In a further embodiment, the humanized antibody comprises a heavy chain comprising the three CDRs of SEQ ID NO:6 and a variable region framework having at least 95% identity to the variable region framework of SEQ ID NO:6, and a light chain comprising the three CDRs of SEQ ID NO:17 and a variable region framework having at least 95% identity to the variable region framework of SEQ ID NO:17. In one embodiment, the humanized antibody comprises a heavy chain comprising the three CDRs of SEQ ID NO:6 and a variable region framework having at least 99% identity to the variable region framework of SEQ ID NO:6.In another embodiment, the humanized antibody comprises a light chain comprising the three CDRs of SEQ ID NO:17 and a variable region framework having at least 99% identity to the variable region framework of SEQ ID NO:17.

[0091] One possible modification is to replace certain residues in the CDRs of the mouse antibody with the corresponding residues from human CDR sequences, typically from the CDRs of the human acceptor sequence used to design the exemplified humanized antibody. In some antibodies, only a portion of the CDRs, i.e., a subset of the CDR residues required for binding, called SDRs, are required to retain binding in the humanized antibody. CDR residues that are not in contact with the antigen and are not in the SDRs can be identified based on previous studies from regions of the Kabat CDRs outside the Chothia hypervariable loops (Chothia, J. Mol. Biol. 196:901, 1987), by molecular modeling, and / or experimentally, or as described in Gonzales et al., Mol. Immunol. 41: 863 (2004) (e.g., residues H60-H65 in CDR H2 are often not required). In such humanized antibodies, where one or more donor CDR residues are absent or where the entire donor CDR is deleted, the amino acid occupying that position may be the amino acid occupying the corresponding position (according to Kabat numbering) in the acceptor antibody sequence. The number of such substitutions of acceptor for donor amino acids in the CDRs to include reflects a balance of competing considerations. Such substitutions may be advantageous to reduce the number of mouse amino acids in the humanized antibody and thus reduce potential immunogenicity. However, substitutions may also result in changes in affinity, and it is preferable to avoid significant affinity losses. The positions of substitutions within the CDRs and the amino acids to be substituted may also be selected empirically.

[0092] Although not preferred, other amino acid substitutions can be made, for example, in framework residues that do not contact CDRs, or even in residues within CDRs that contact some potential CDRs. The substitutions made in the variant humanized sequence are often conservative with respect to the replaced HCLG amino acids. Preferably, the substitutions (conservative or not) with respect to HCLG do not significantly affect the binding affinity or potency of the humanized mAb, i.e., its ability to bind to human αvβ6 and inhibit the proliferation of cancer cells.

[0093] Selection of constant regions The heavy and light chain variable regions of the humanized antibody may be linked to at least a portion of a human constant region. The choice of constant region depends, in part, on whether antibody-dependent cell-mediated cytotoxicity, antibody-dependent cellular phagocytosis, and / or complement-dependent cytotoxicity are desired. For example, human isotypes IgG1 and IgG3 have strong complement-dependent cytotoxicity, human isotype IgG2 has weak complement-dependent cytotoxicity, and human IgG4 lacks complement-dependent cytotoxicity. Human IgG1 and IgG3 also induce stronger cell-mediated effector functions than human IgG2 and IgG4. The light chain constant region may be lambda or kappa. Antibodies may be expressed as tetramers containing two light chains and two heavy chains, as separate heavy and light chains, as Fab, Fab', F(ab')2, and Fv, or as single chain antibodies in which the heavy and light chain variable domains are linked through a spacer.

[0094] Human constant regions exhibit allotypic and isoallotypic diversity among different individuals, i.e., the constant regions can differ in different individuals at one or more polymorphic positions. Isoallotypes differ from allotypes in that sera that recognize an isoallotype bind to non-polymorphic regions of one or more other isotypes.

[0095] One or several amino acids at the amino or carboxy termini of the light and / or heavy chains, such as the C-terminal lysine of the heavy chain, may be deleted or derivatized in a proportion or all of the molecules. Substitutions may be made in the constant regions to reduce or increase effector functions such as complement-mediated cytotoxicity or ADCC (see, e.g., Winter et al., U.S. Pat. No. 5,624,821; Tso et al., U.S. Pat. No. 5,834,597; and Lazar et al., Proc. Natl. Acad. Sci. USA 103: 4005, 2006) or to extend half-life in humans (see, e.g., Hinton et al., J. Biol. Chem. 279: 6213, 2004).

[0096] Exemplary substitutions include amino acid substitutions of native amino acids to cysteine ​​residues introduced at amino acid positions 234, 235, 237, 239, 267, 298, 299, 326, 330, or 332, preferably the S239C mutation in human IgG1 isotype (US 20100158909). The presence of the additional cysteine ​​residue allows interchain disulfide bond formation. Such interchain disulfide bond formation may reduce the affinity of the Fc region-FcγR binding interaction by creating steric hindrance. The cysteine ​​residue(s) introduced at or adjacent to the Fc region of the IgG constant region may also serve as a site for conjugation with a therapeutic agent (i.e., attachment of a cytotoxic agent using a thiol-specific reagent such as a maleimide derivative of the drug). The presence of the therapeutic agent may create steric hindrance that may further reduce the affinity of the Fc region-FcγR binding interaction. Other substitutions at any of positions 234, 235, 236, and / or 237 result in reduced affinity for Fcγ receptors, particularly the FcγRI receptor (see, e.g., US 6,624,821, US 5,624,821).

[0097] The in vivo half-life of an antibody can also affect its effector function. The half-life of an antibody can be increased or decreased to modify its therapeutic activity. FcRn is a receptor structurally similar to MHC class I antigens that non-covalently associate with β2-microglobulin. FcRn regulates the catabolism of IgG and their transcytosis across tissues (Ghetie and Ward, 2000, Annu. Rev. Immunol. 18:739- 766; Ghetie and Ward, 2002, Immunol. Res. 25:97-113). IgG-FcRn interaction occurs at pH 6.0 (pH of intracellular vesicles) but not at pH 7.4 (pH of blood). This interaction allows IgG to be recycled back into the circulation (Ghetie and Ward, 2000, Ann. Rev. Immunol. 18:739-766; Ghetie and Ward, 2002, Immunol. Res. 25:97-113). Regions on human IgG1 involved in FcRn binding have been mapped (Shields et al, 2001, J. Biol. Chem. 276:6591-604). Alanine substitutions at positions Pro238, Thr256, Thr307, Gln311, Asp312, Glu380, Glu382, or Asn434 of human IgG1 enhance FcRn binding (Shields et al, 2001, J. Biol. Chem. 276:6591-604). IgG1 molecules containing these substitutions have a longer serum half-life. As a result, these modified IgG1 molecules are able to perform their effector functions and therefore exert their therapeutic effect for a longer period of time compared to unmodified IgG1. Other exemplary substitutions to increase binding to FcRn include Gln at position 250 and / or Leu at position 428. EU numbering is used for all positions in the constant region.

[0098] The oligosaccharide covalently attached to the conserved Asn297 is responsible for the ability of the Fc region of IgG to bind to FcγR (Lund et al, 1996, J. Immunol. 157:4963-69; Wright and Morrison, 199', Trends Biotechnol. 15:26-31). Engineering this glycoform on IgG can significantly improve IgG-mediated ADCC. Adding bisecting N-acetylglucosamine modifications to this glycoform (Umana et al, 1999, Nat. Biotechnol. 17:176-180; Davies et al, 2001, Biotech. Bioeng. 74:288-94) or removing fucose from this glycoform (Shields et al, 2002, J. Biol. Chem. 277:26733-40; Shinkawa et al, 2003, J. Biol. Chem. 278:6591-604; Niwa et al., 2004, Cancer Res. 64:2127-33) are two examples of IgG Fc engineering that improves binding between IgG Fc and FcγR, thereby enhancing Ig-mediated ADCC activity.

[0099] Systematic substitution of solvent-exposed amino acids in the human IgG1 Fc region has generated IgG variants with altered FcγR binding affinity (Shields et al, 2001, J. Biol. Chem. 276:6591-604). Compared to the parent IgG1, a subset of these variants involving substitutions of Thr256 / Ser298, Ser298 / Glu333, Ser298 / Lys334, or Ser298 / Glu333 / Lys334 to Ala show both increased binding affinity to FcγR and ADCC activity (Shields et al, 2001, J. Biol. Chem. 276:6591-604; Okazaki et al, 2004, J. Mol. Biol. 336:1239-49).

[0100] The complement fixation activity (both C1q binding and CDC activity) of antibodies can be improved by substitutions at Lys326 and Glu333 (Idusogie et al., 2001, J. Immunol. 166:2571-2575). The same substitutions in the human IgG2 backbone can convert antibody isotypes that bind poorly to C1q and have a significant lack of complement activation activity into those that can both bind C1q and mediate CDC (Idusogie et al., 2001, J. Immunol. 166:2571-75). Several other methods have also been applied to improve the complement fixation activity of antibodies. For example, grafting the 18 amino acid carboxyl-terminal tail of IgM onto the carboxyl terminus of IgG enhances their CDC activity. This is observed even with IgG4, which normally has no detectable CDC activity (Smith et al, 1995, J. Immunol. 154:2226-36). Substitution of Ser444, located near the carboxy terminus of the IgG1 heavy chain, with Cys induced tail-to-tail dimerization of IgG1, which increased CDC activity 200-fold over monomeric IgG1 (Shopes et al, 1992, J. Immunol. 148:2918-22). In addition, construction of bispecific diabodies with specificity for C1q also confers CDC activity (Kontermann et al., 1997, Nat. Biotech. 15:629-31).

[0101] Complement activity can be reduced by mutating at least one of amino acid residues 318, 320, and 322 of the heavy chain to a residue having a different side chain, such as Ala. Other alkyl-substituted non-ionic residues, such as Gly, Ile, Leu, or Val, or aromatic non-polar residues, such as Phe, Tyr, Trp, and Pro, in place of any of the three residues also reduce or abolish C1q binding. Ser, Thr, Cys, and Met can be used at residues 320 and 322, rather than residue 318, to reduce or abolish C1q binding activity.

[0102] The replacement of residue 318 (Glu) with a polar residue can modify the C1q binding activity without abolishing it. The replacement of residue 297 (Asn) with Ala eliminates the lytic activity but only slightly reduces the affinity for C1q (approximately 3-fold weakening). This modification destroys the glycosylation site and destroys the presence of carbohydrates required for complement activation. Any other substitution at this site also destroys the glycosylation site. The D270A, K322A, P329A, and P311S mutations and any combination thereof also reduce C1q binding (see WO 06 / 036291). The L234A / L235A mutation (or LALA mutation) also reduces C1q binding, as well as FcyR binding.

[0103] Reference to a human constant region includes constant regions with any naturally occurring allotype or any permutation of residues occupying polymorphic positions in the naturally occurring allotypes, and there may be up to 1, 2, 5, or 10 mutations, such as those listed above, relative to a naturally occurring human constant region to reduce Fc gamma receptor binding or increase binding to FcRn.

[0104] V. Expression of Recombinant Antibodies Humanized antibodies can typically be produced by recombinant expression. Recombinant polynucleotide constructs typically contain expression control sequences operably linked to the coding sequences of the antibody chains, including naturally associated or heterologous promoter regions. Preferably, the expression control sequences are eukaryotic promoter systems in vectors capable of transforming or transfecting eukaryotic host cells. Once the vector has been incorporated into an appropriate host, the host is maintained under conditions suitable for high level expression of the nucleotide sequences, and the recovery and purification of the cross-reacting antibodies.

[0105] Mammalian cells are preferred hosts for expressing nucleotide segments encoding immunoglobulins or fragments thereof. See Winnacker, From Genes to Clones, (VCH Publishers, NY, 1987). Several suitable host cell lines capable of screening intact heterologous proteins have been developed in the art, including CHO cell lines (e.g., DG44), various COS cell lines, HeLa cells, HEK293 cells, L cells, and non-antibody producing myelomas (including Sp2 / 0 and NS0). Preferably, the cells are non-human. Expression vectors for these cells may include expression control sequences such as an origin of replication, a promoter, an enhancer (Queen et al., Immunol. Rev. 89:49 (1986)), and necessary processing information sites such as ribosome binding sites, RNA splice sites, polyadenylation sites, and transcription termination sequences. Preferred expression control sequences are promoters derived from endogenous genes, cytomegalovirus, SV40, adenovirus, bovine papilloma virus, etc. See Co et al., J. Immunol. 148:1149 (1992).

[0106] Once expressed, antibodies can be purified according to standard procedures in the art, including HPLC purification, column chromatography, gel electrophoresis, and the like (see generally, Scopes, Protein Purification (Springer-Verlag, NY, 1982)).

[0107] VI. Nucleic acids The present invention further provides nucleic acids encoding any of the above humanized heavy and light chains. Typically, the nucleic acid also encodes a signal peptide fused to the mature heavy and light chains. The coding sequence on the nucleic acid may be in operative linkage with regulatory sequences to ensure expression of the coding sequence, such as promoters, enhancers, ribosome binding sites, transcription termination signals, etc. The nucleic acids encoding the heavy and light chains may occur in isolated form or may be cloned into one or more vectors. The nucleic acids may be synthesized, for example, by solid-state synthesis or PCR of overlapping oligonucleotides. The nucleic acids encoding the heavy and light chains may be linked, for example, as one adjacent nucleic acid in an expression vector, or may be separate, for example, each cloned into its own expression vector.

[0108] In some embodiments, also provided herein is a nucleic acid encoding an anti-αvβ6 antibody or antigen-binding fragment thereof described herein. Further provided herein is a vector comprising a nucleic acid encoding an anti-αvβ6 antibody or antigen-binding fragment thereof described herein. Further provided herein is a host cell expressing a nucleic acid encoding an anti-αvβ6 antibody or antigen-binding fragment thereof described herein. Further provided herein is a host cell comprising a vector comprising a nucleic acid encoding an anti-αvβ6 antibody or antigen-binding fragment thereof described herein.

[0109] The anti-αvβ6 antibodies described herein can be prepared by well-known recombinant techniques using well-known expression vector systems and host cells. In one embodiment, the antibodies are prepared in CHO cells using the GS expression vector system as disclosed in De la Cruz Edmunds et al., 2006, Molecular Biotechnology 34; 179-190, EP216846, U.S. Pat. No. 5,981,216, WO 87 / 04462, EP323997, U.S. Pat. No. 5,591,639, U.S. Pat. No. 5,658,759, EP338841, U.S. Pat. No. 5,879,936, and U.S. Pat. No. 5,891,693.

[0110] The monoclonal anti-αvβ6 antibodies described herein may be produced by the hybridoma method first described, for example, in Kohler et al., Nature, 256, 495 (1975), or may be produced by recombinant DNA methods. Monoclonal antibodies may also be isolated from phage antibody libraries, for example, using the techniques described in Clackson et al., Nature, 352, 624-628 (1991) and Marks et al., JMol, Biol., 222(3):581-597 (1991). Monoclonal antibodies may be obtained from any suitable source. Thus, for example, monoclonal antibodies may be obtained from hybridomas prepared from mouse splenic B cells obtained from mice immunized with the antigen of interest, e.g., cells expressing the antigen on their surface, or in the form of a nucleic acid encoding the antigen of interest. Monoclonal antibodies can also be obtained from hybridomas obtained from antibody-expressing cells of immunized humans or non-human mammals such as rats, dogs, and primates.

[0111] VII. Antibody Drug Conjugates Anti-αvβ6 antibodies can be conjugated with cytotoxic or cytostatic moieties (including pharma- ceutically compatible salts thereof) to form antibody-drug conjugates (ADCs). Particularly suitable moieties for conjugation with antibodies are cytotoxic agents (e.g., chemotherapeutic agents), prodrug-converting enzymes, radioisotopes or compounds, or toxins (these moieties are collectively referred to as therapeutic agents). For example, anti-αvβ6 antibodies can be conjugated with cytotoxic agents, such as chemotherapeutic agents, or toxins (e.g., cytostatic or cytocidal agents, such as abrin, ricin A, Pseudomonas exotoxin, or diphtheria toxin).

[0112] The anti-αvβ6 antibody can be conjugated to a prodrug-converting enzyme. The prodrug-converting enzyme can be recombinantly fused to the antibody or chemically conjugated to the antibody using known methods. Exemplary prodrug-converting enzymes are carboxypeptidase G2, beta-glucuronidase, penicillin-V-amidase, penicillin-G-amidase, β-lactamase, β-glucosidase, nitroreductase, and carboxypeptidase A.

[0113] Techniques for conjugating therapeutic agents to proteins, particularly antibodies, are well known. (For example, Arnon et al, "Monoclonal Antibodies For Immunotargeting Of Drugs In Cancer Therapy," Monoclonal Antibodies And Cancer Therapy (Reisfeld et al., ed., Alan R. Liss, Inc., 1985); Hellstrom et al, "Antibodies For Drug Delivery," Controlled Drug Delivery (Robinson et al., ed., Marcel Dekker, Inc., 2nd ed. 1987); Thorpe, "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review," Monoclonal Antibodies '84: Biological And Clinical Applications (Pinchera et al., ed., 1985); "Analysis, Results, and Future Prospective of the Therapeutic Use of Radiolabeled Antibody In Cancer Therapy," Monoclonal Antibodies For Cancer Detection And Therapy (Baldwin et al., ed., Academic Press, 1985); Thorpe et al. 1982, Immunol. Rev. 62:119-58; see also PCT Publication WO 89 / 12624).

[0114] The therapeutic agent may be conjugated in a manner that reduces its activity unless it is cleaved from the antibody (e.g., by hydrolysis, antibody degradation, or a cleavage agent). Such a therapeutic agent is attached to the antibody by a cleavable linker that is susceptible to cleavage in the intracellular environment of an αvβ6-expressing cancer cell, but not substantially in the extracellular environment, such that the conjugate is cleaved from the antibody when it is internalized by the αvβ6-expressing cancer cell (e.g., in an endosomal environment, or, e.g., in a lysosomal environment, or in a caveolear environment, by pH sensitivity or protease sensitivity).

[0115] Typically, the ADC comprises a linker region between the therapeutic agent and the anti-αvβ6 antibody. As described above, typically, the linker can be cleavable under intracellular conditions such that cleavage of the linker releases the therapeutic agent from the antibody in the intracellular environment (e.g., in a lysosome or endosome or caveolea). The linker can be, for example, a peptidyl linker that is cleaved by an intracellular peptidase or protease enzyme, including a lysosomal or endosomal protease. Typically, the peptidyl linker is at least 2 amino acids long or at least 3 amino acids long. Cleavage agents can include cathepsins B and D, and plasmin (see, e.g., Dubowchik and Walker, 1999, Pharm. Therapeutics 83:67-123). Most typical is a peptidyl linker that is cleavable by an enzyme present in αvβ6-expressing cells. For example, a peptidyl linker (e.g., a linker comprising a Phe-Leu or Gly-Phe-Leu-Gly peptide) that is cleavable by the thiol-dependent protease cathepsin B, which is highly expressed in cancerous tissues, can be used. Other such linkers are described, for example, in U.S. Pat. No. 6,214,345. In certain embodiments, the peptide linker cleavable by an intracellular protease includes a Val-Cit linker or a Phe-Lys dipeptide (see, for example, U.S. Pat. No. 6,214,345, which describes the synthesis of doxorubicin with a Val-Cit linker). The advantage of using intracellular proteolytic release of a therapeutic agent is that the agent is usually weakened when conjugated, and the serum stability of the conjugate is usually high.

[0116] The cleavable linker may be pH sensitive, i.e., sensitive to hydrolysis at a certain pH value. Typically, the pH sensitive linker is hydrolyzable under acidic conditions. For example, an acid-labile linker (e.g., hydrazone, semicarbazone, thiosemicarbazone, cis-aconitic amide, orthoester, acetal, ketal, etc.) that is hydrolyzable in lysosomes can be used (see, for example, U.S. Patent Nos. 5,122,368; 5,824,805; 5,622,929; Dubowchik and Walker, 1999, Pharm. Therapeutics 83:67-123; Neville et al., 1989, Biol. Chem. 264:14653-14661). Such linkers are relatively stable under neutral pH conditions such as in blood, but are unstable at pH levels lower than pH 5.5 to 5.0, which is the approximate pH of lysosomes. In certain embodiments, the hydrolyzable linker is a thioether linker (such as a thioether that is attached to a therapeutic agent via an acylhydrazone bond (see, eg, US Pat. No. 5,622,929).

[0117] Other linkers are cleavable under reducing conditions (eg, disulfide linkers). Disulfide linkers include SATA (N-succinimidyl-S-acetylthioacetate), SPDP (N-succinimidyl-3-(2-pyridyldithio)propionate), SPDB (N-succinimidyl-3-(2-pyridyldithio)butyrate) and SMPT (N-succinimidyl-oxycarbonyl-alpha-methyl-alpha-(2-pyridyl-dithio)toluene), linkers which can be made using SPDB and SMPT (see, e.g., Thorpe et al., 1987, Cancer Res. 47:5924-5931; Wawrzynczak et al., Immunoconjugates: Antibody Conjugates in Radioimagery and Therapy of Cancer (CW Vogel ed., Oxford U. Press, 1987; see also U.S. Pat. No. 4,880,935).

[0118] The linker may be a malonic acid linker (Johnson et al., 1995, Anticancer Res. 15:1387-93), a maleimidobenzoyl linker (Lau et al., 1995, Bioorg-Med-Chem. 3(10):1299-1304), or a 3'-N-amide analog (Lau et al., 1995, Bioorg-Med-Chem. 3(10):1305-12). The linker may also be a malonic acid linker (Johnson et al, 1995, Anticancer Res. 15:1387-93), a maleimidobenzoyl linker (Lau et al, 1995, Bioorg-Med-Chem. 3(10):1299-1304), or a 3'-N-amide analog (Lau et al, 1995, Bioorg-Med-Chem. 3(10):1305-12).

[0119] The linker can also be a non-cleavable linker, such as a maleimide-alkylene or maleimide-aryl linker that is directly attached to the therapeutic agent (e.g., a drug). The active drug linker is released upon degradation of the antibody.

[0120] The linker may promote cellular internalization. The linker may promote cellular internalization when conjugated to a therapeutic agent (i.e., in the context of a linker-therapeutic agent portion of an ADC or ADC derivative described herein). Alternatively, the linker may promote cellular internalization when conjugated to both a therapeutic agent and an anti-αvβ6 antibody (i.e., in the context of an ADC described herein).

[0121] The anti-αvβ6 antibody can be conjugated to the linker through the heteroatoms of the antibody. These heteroatoms can be present on the antibody in its natural state or can be introduced into the antibody. In some embodiments, the anti-αvβ6 antibody is conjugated to the linker through the nitrogen atom of a lysine residue. In other embodiments, the anti-αvβ6 antibody is conjugated to the linker through the sulfur atom of a cysteine ​​residue. The cysteine ​​residue can be natural or engineered into the antibody. Methods for conjugating the antibody to the linker and drug linker through lysine and cysteine ​​residues are known in the art.

[0122] Exemplary antibody drug conjugates include auristatin-based antibody drug conjugates (i.e., the drug moiety is an auristatin drug). Auristatins have been shown to bind to tubulin and interfere with microtubule dynamics and nuclear and cell division, and have anti-cancer activity. Typically, auristatin-based antibody drug conjugates include a linker between the auristatin drug and the anti-αvβ6 antibody. The linker can be, for example, a cleavable linker (e.g., a peptidyl linker, a carbohydrate linker) or a non-cleavable linker (e.g., a linker that is released by degradation of the antibody). Auristatins include (but are not limited to) auristatin T, MMAF, and MMAE. The synthesis and structures of exemplary auristatins are described in U.S. Publication Nos. 7,659,241, 7,498,298, 2009-0111756, 2009-0018086, and 7,968,687, each of which is incorporated herein by reference in its entirety and for all purposes.

[0123] Exemplary auristatin-based antibody drug conjugates include the vcMMAE (or 1006), vcMMAF and mcMMAF antibody drug conjugates shown below, where p is drug loading, Ab is an anti-αvβ6 antibody described herein, and val-cit or "vc" represents a valine-citrulline dipeptide: [ka] [ka] or its pharma- ceutically acceptable salts. Drug loading is represented by p, which is the number of drug linker molecules per antibody. When referring to αvβ6-targeted antibody drug conjugates, the subscript p represents drug loading, which may represent the number of molecules of drug linker molecules attached to individual antibody molecules, in which case it is an integer value, or represents average drug loading, in which case it may be an integer or non-integer value, but typically a non-integer value. Average drug loading represents the average number of drug linker molecules per antibody in a population. Often, but not always, when referring to antibodies, such as monoclonal antibodies, one is referring to a population of antibody molecules. In compositions that include a population of antibody drug conjugate molecules, average drug loading is an important quality attribute, since it determines the amount of drug that can be delivered to target cells. The percentage of unconjugated antibody molecules in the composition is included in the average drug loading value.

[0124] In a preferred aspect of the invention, when referring to a composition comprising a population of antibody drug conjugate compounds, the average drug loading is 1 to about 16, preferably about 2 to about 14, more preferably about 2 to about 10. In one embodiment, the DAR is about 2 to about 5. In a further embodiment, the DAR is 4. In another embodiment, the DAR is about 6 to about 10. In a further embodiment, the DAR is 8. The average number of drugs per antibody in the preparation can be characterized by conventional means such as mass spectrometry, HIC, ELISA assay, HPLC, etc. In some embodiments, the anti-αvβ6 antibody is attached to the drug linker via a cysteine ​​residue in the antibody. In some embodiments, the cysteine ​​residue is engineered into the antibody. In other embodiments, the cysteine ​​residue is an interchain disulfide cysteine ​​residue.

[0125] In some embodiments, incorporation of a polyethylene glycol polymer as a side chain into a cleavable β-glucuronide MMAE drug linker results in antibody drug conjugates with reduced plasma clearance and increased anti-tumor activity in xenograft models when compared to non-PEGylated controls. Thus, a particularly advantageous drug linker for attaching the antibodies of the invention has the following formula V: [ka] or a pharma- ceutically acceptable salt thereof.

[0126] The preferred stereochemistry for such drug linkers is shown below in Formula Va: [ka] or a pharma- ceutically acceptable salt thereof, wherein, for formulas V and Va, Z represents an organic moiety having a reactive site capable of reacting with a functional group on an antibody to form a covalent bond therewith, n is in the range of 8 to 36, most preferably in the range of 8 to 14 (most preferably 12), R 21 is a capping unit of a polyethylene glycol moiety, preferably -CH3 or -CH2CH2CO2H.

[0127] Preferred Z moieties are maleimide-containing moieties. Particularly preferred Z moieties are the following drug-linkers: [ka] [ka] or a pharma- ceutically acceptable salt thereof.

[0128] The preferred stereochemistry for such drug linkers is as follows: [ka] or a pharma- ceutically acceptable salt thereof, wherein, for formulas VI, VIa, VII, and VIIa, n is in the range of 8 to 36, most preferably in the range of 8 to 14 (most preferably 12); R PR is hydrogen or a protecting group, e.g., an acid labile protecting group, e.g., BOC; R 21 is a capping unit of a polyethylene glycol moiety, preferably -CH3 or -CH2CH2CO2H.

[0129] As mentioned above, R PR can be hydrogen or a protecting group. As used herein, a protecting group refers to a group that selectively blocks, either temporarily or permanently, a reactive site of a multifunctional compound. A protecting group is suitable if it can be removed under reaction conditions necessary to effect a desired chemical transformation elsewhere in the molecule, and, if desired, during purification of the newly formed molecule, that can prevent or avoid undesired side reactions or premature loss of the protecting group and that do not adversely affect the structural or stereochemical integrity of the newly formed molecule. Suitable amine protecting groups include acid labile nitrogen protecting groups, including those provided by Isidro-Llobel et al. "Amino acid-protecting groups" Chem. Rev. (2009) 109: 2455-2504. Typically, acid labile nitrogen protecting groups convert a primary or secondary amino group to its corresponding carbamate and include t-butyl, allyl, and benzyl carbamates.

[0130] As mentioned above, R 21 is the capping unit of the polyethylene glycol moiety. As will be appreciated by those skilled in the art, the polyethylene glycol unit can be end-capped with a wide variety of organic moieties, typically those that are relatively unreactive. Alkyl and substituted alkyl groups are preferred.

[0131] A particularly preferred average drug loading for MMAE PEGylated ADCs, such as those exemplified herein, is about 8. In an exemplary embodiment, the drug linker is conjugated to a systemin residue of a reduced interchain disulfide. In some aspects, the actual drug loading of individual antibody molecules in a population of antibody drug conjugate compounds is 1-10 (or 6-10, or 6-8), with a predominant drug loading of 8. For example, higher drug loading can be obtained when the drug linker is conjugated to an introduced cysteine ​​residue (such as a cysteine ​​residue introduced at position 239 according to the EU index) in addition to the interchain disulfide.

[0132] Exemplary ADCs include the following: [ka] [ka] [ka] or a pharma- ceutically acceptable salt thereof, in which n is in the range of 8 to 36, most preferably in the range of 8 to 14 (most preferably 12); PR is hydrogen or a protecting group, e.g., an acid labile protecting group, e.g., BOC; R 21 is a capping unit of a polyethylene glycol moiety, preferably -CH3 or -CH2CH2CO2H; Ab represents an anti-αvβ6 antibody; and p represents an integer in the range of 1 to 16, preferably 1 to 14, 6 to 12, 6 to 10, or 8 to 10 when referring to an individual antibody molecule, or an average drug loading of about 4 or about 6 to about 14, preferably about 8 when referring to a population of antibody molecules.

[0133] As mentioned above, the PEG (polyethylene glycol) portion of the drug linker may range from 8 to 36, although PEGs of 12 ethylene oxide units have been found to be particularly preferred. It has been found that longer PEG chains may result in slower clearance, while shorter PEG chains may result in reduced activity. Thus, in all of the above embodiments, the subscript n is preferably 8-14, 8-12, 10-12, or 10-14, and most preferably 12.

[0134] Polydisperse PEGs, monodisperse PEGs, and individual PEGs can be used to make the PEGylated antibody drug conjugates of the present invention. Polydisperse PEGs are a mixture of heterogeneous sizes and molecular weights, while monodisperse PEGs are typically purified from a heterogeneous mixture, thus providing a single chain length and molecular weight. The preferred PEG units are individual PEGs, which are compounds that are synthesized stepwise and do not go through a polymerization process. Individual PEGs provide single molecules with defined and specific chain lengths. As with the subscript "p", when referring to a population of antibody drug conjugates, the value of the subscript "n" can be an average number, and can be an integer or a non-integer.

[0135] In a preferred embodiment, covalent attachment of the antibody to the drug linker is achieved through a sulfhydryl functional group of the antibody, which interacts with a maleimide functional group of the drug linker to form a thio-substituted succinimide. The sulfhydryl functional group may be present on the Ligand unit in the native state of the Ligand, e.g., a naturally occurring residue (interchain disulfide residue), or may be introduced into the Ligand via chemical modification, or by biological alteration, or a combination of the two. It is understood that the antibody-substituted succinimide may exist in hydrolyzed form(s). For example, in a preferred embodiment, the ADC, when conjugated to an antibody, is [ka] or when attached to an antibody, comprises a succinimide moiety represented by the structure [ka] where the wavy line indicates the connection to the remainder of the drug-linker.

[0136] In some embodiments, the anti-αvβ6 antibody of the invention is conjugated to monomethylauristatin E via a MDpr-PEG(12)-gluc linker to form an antibody drug conjugate having the following structure: [ka] or a pharma- ceutically acceptable salt thereof, wherein n is in the range of 8 to 36, most preferably in the range of 8 to 14 (most preferably 12); R PR is hydrogen or a protecting group, e.g., an acid labile protecting group, e.g., BOC; R 21 is a capping unit of a polyethylene glycol moiety, preferably -CH3 or -CH2CH2CO2H; Ab represents an anti-αvβ6 antibody; and p represents an integer in the range of 1 to 16, preferably 1 to 14, 6 to 12, 6 to 10, or 8 to 10 when referring to an individual antibody molecule, or an average drug loading of about 4 or about 6 to about 14, preferably about 8 when referring to a population of antibody molecules.

[0137] Exemplary antibody drug conjugates also include camptothecin-based antibody drug conjugates (i.e., the drug moiety is a camptothecin drug). Camptothecin is a topoisomerase inhibitor that has been shown to have anti-cancer activity. Typically, camptothecin-based antibody drug conjugates include a linker between the camptothecin drug and the anti-αvβ6 antibody. The linker can be, for example, a cleavable linker (e.g., a peptidyl linker, a carbohydrate linker) or a non-cleavable linker (e.g., a linker that is released by degradation of the antibody). The synthesis and structure of exemplary camptothecin drug linkers are described in PCT / US19 / 025968 (filed April 5, 2019), the entirety of which is incorporated herein by reference for all purposes.

[0138] Exemplary anti-αvβ6 antibody drug conjugates include camptothecin antibody drug conjugates as follows, where p represents the drug loading and Ab represents the anti-αvβ6 antibody:

[0139] In some embodiments, a camptothecin ADC has the formula (IC): [ka] or a pharma- ceutically acceptable salt thereof; During the ceremony, Ab is an anti-αvβ6 antibody, y is 1, 2, 3, or 4, or is 1 or 4, and z is an integer from 2 to 12, or is 2, 4, 8, or 12; and p is 1 to 16.

[0140] In some aspects of these embodiments, p is 2, 3, 4, 5, 6, 7, 8, 9, or 10. In some aspects, p is 2, 4, or 8.

[0141] In some embodiments, a camptothecin ADC has the formula: [ka] or a pharma- ceutically acceptable salt thereof; In the formula, p is 2, 4 or 8, and preferably p is 8.

[0142] In some embodiments, a camptothecin ADC has the formula: [ka] or a pharma- ceutically acceptable salt thereof; In the formula, p is 2, 4 or 8, and preferably p is 8.

[0143] In some embodiments, the camptothecin drug linker has the formula: [ka] or a pharma- ceutically acceptable salt thereof; During the ceremony, y is 1, 2, 3, or 4, or is 1 or 4, and z is an integer from 2 to 12, or is 2, 4, 8, or 12.

[0144] In some embodiments, the camptothecin drug linker has the formula: [ka]

[0145] In some embodiments, the camptothecin drug linker has the formula: [ka]

[0146] In some embodiments, the camptothecin drug linker has the formula: [ka]

[0147] Other exemplary antibody drug conjugates include maytansinoid antibody drug conjugates (i.e., the drug moiety is a maytansinoid drug), and benzodiazepine antibody drug conjugates (i.e., the drug moiety is a benzodiazepine (e.g., pyrrolo[1,4]benzodiazepine dimers (PDB dimers), indolinobenzodiazepine dimers, and oxazolidinobenzodiazepine dimers).

[0148] In some embodiments, a PBD dimer for use in the present invention is represented by formula I. The preferred stereochemistry of the PBD dimer is as shown in formula Ia: [ka] or a pharmaceutical salt, solvate, or solvate of the salt, wherein the subscript n is 1 or 3.

[0149] The solvates of formulas (I) and (Ia) are typically formed by adding water or an alcohol solvent across the imine functional groups of one or both of the PBD monomers to form the carbinolamine(s) and / or carbinolamine ether. For example, at the N10-C11 positions, there may be an imine (N=C), a carbinolamine (NH-CH(OH)), or a carbinolamine ether (NH-CH(OMe)), as represented by formulas I' and Ia' below: [ka] wherein: (a)R 10 is H and R 11 is OH or OR A where R A is saturated C 1-4 alkyl (preferably methyl); or (b)R 10 and R 11 form a nitrogen-carbon double bond between the nitrogen atom and the carbon atom to which they are attached; or (c)R 10 One of them is H and the other is R 11 is OH or OR A where R A is saturated C 1-4 alkyl (preferably methyl); and R 10 The other and R 11 form a nitrogen-carbon double bond between the nitrogen atom and the carbon atom to which they are attached.

[0150] The PBD dimer of formula I or Ia (or a pharmaceutical salt, solvate, or solvate of a salt thereof) is typically linked to an antibody via a linker unit LU. The linker unit acts to release the PBD dimer of formula I or Ia (or a pharmaceutical salt, solvate, or solvate of a salt thereof) at a target site (e.g., inside a cancer cell). PBD drug-linker compounds for use in the present invention are represented below by formula II (preferred stereochemistry shown in IIa), where LU is the linker unit. The linker unit can be, for example, a cleavable peptide linker unit (e.g., a linker comprising a valine-alanine peptide) or a cleavable disulfide linker unit: [ka] or a pharmaceutical salt, solvate, or solvate of the salt, wherein the subscript n is 1 or 3.

[0151] Preferred PBD drug-linker compounds for use in the present invention are represented by Formula III below: [ka] or a pharmaceutical salt, solvate, or solvate of the salt, wherein the subscript n is 1 or 3 and the subscript m is an integer from 2 to 5.

[0152] The PBD drug-linker is conjugated to an anti-αvβ6 antibody to generate an αvβ6-targeted antibody drug conjugate. For example, the antibody can be conjugated to a drug linker of formula II or formula III. Exemplary αvβ6-targeted antibody drug conjugates are shown below in formulas IV, IVa, and IVb: [ka] or a pharmaceutical salt, solvate, or solvate of the salt, wherein the subscript n is 1 or 3, the subscript m is an integer from 2 to 5, and the subscript p is 1 to 4.

[0153] Useful classes of cytotoxic agents for conjugation to anti-αvβ6 antibodies include, for example, antitubulin agents, DNA minor groove binders, DNA replication inhibitors, chemotherapy sensitizers, etc. Examples of other classes of cytotoxic agents include anthracyclines, auristatins, camptothecins, duocarmycins, etoposide, maytansinoids, and vinca alkaloids. Some examples of cytotoxic agents include auristatins (e.g., auristatin T, auristatin E, AFP, monomethylauristatin F (MMAF), lipophilic monomethylauristatin F, monomethylauristatin E (MMAE)), DNA minor groove binding agents (e.g., enediynes and lexitropsins), duocarmycins, taxanes (e.g., paclitaxel and docetaxel), vinca alkaloids, nicotinamide phosphoribosyltransferase inhibitors (NAMPTi), tubulysin M, doxorubicin, morpholinodoxorubicin, and cyanomorpholinodoxorubicin.

[0154] The cytotoxic agent can be a chemotherapeutic drug, such as doxorubicin, paclitaxel, melphalan, vinca alkaloids, methotrexate, mitomycin C, or etoposide. The drug can also be a CC-1065 analog, a calicheamicin, a maytansine, an analog of dolastatin 10, rhizoxin, or palytoxin.

[0155] The cytotoxic agent may be an auristatin. The auristatin may be an auristatin E derivative, such as an ester formed between auristatin E and a keto acid. For example, auristatin E may be reacted with paraacetylbenzoic acid or benzoylvaleric acid to produce AEB and AEVB, respectively. Other exemplary auristatins include auristatin T, AFP, MMAF, and MMAE. The synthesis and structures of various auristatins are described, for example, in US 2005-0238649 and US 2006-0074008.

[0156] The cytotoxic agent can be a DNA minor groove binding agent. (See, e.g., U.S. Patent No. 6,130,237.) For example, the minor groove binding agent can be a CBI compound or an enediyne (e.g., calicheamicin).

[0157] The cytotoxic or cytostatic agent can be an antitubulin drug. Examples of antitubulin drugs include taxanes (e.g., Taxol® (paclitaxel), Taxotere® (docetaxel)), T67 (Tularik), vinca alkaloids (e.g., vincristine, vinblastine, vindesine, and vinorelbine), and auristatins (e.g., auristatin E, AFP, MMAF, MMAE, AEB, AEVB). Examples of auristatins are shown in Formulae III-XIII below. Other suitable antitubulin drugs include, for example, baccatin derivatives, taxane analogs (e.g., epothilone A and B), nocodazole, colchicine and colcemid, estramustine, cryptophycin, cemadotin, maytansinoids, combretastatins, discodermolide, and erytherobin.

[0158] The cytotoxic agent can be a maytansinoid, another group of antitubulin drugs (e.g., DM1, DM2, DM3, DM4). For example, the maytansinoid can be maytansine or a maytansine containing a drug linker, such as DM-1 or DM-4 (ImmunoGen, Inc.; see also Chari et al., 1992, Cancer Res.).

[0159] VIII. Therapeutic applications (therapeutic uses) The antibodies of the present invention, alone or as anti-αvβ6 antibody drug conjugates thereof, can be used to treat cancer. Some of such cancers exhibit detectable levels of αvβ6 measured at either the protein (e.g., by immunoassay using one of the exemplified antibodies) or mRNA levels. Some of such cancers exhibit elevated levels of αvβ6 compared to non-cancerous tissue of the same type, preferably from the same patient. An exemplary level of αvβ6 on cancer cells suitable for treatment is 5000-500,000 αvβ6 molecules per cell, although higher or lower levels may be treated. Optionally, αvβ6 levels in the cancer are measured prior to administering treatment.

[0160] Examples of cancers associated with αvβ6 expression and suitable for treatment include non-small cell lung cancer (NSCLC) (squamous cell carcinoma and adenocarcinoma), head and neck cancer (including head and neck squamous cell carcinoma), esophageal cancer, breast cancer (including invasive breast cancer), ovarian cancer, bladder cancer (including urothelial carcinoma), skin cancer (squamous cell carcinoma, i.e., SCC), renal cancer (including clear cell renal cell carcinoma, papillary renal cell carcinoma, chromophobe renal cell carcinoma), cervical cancer, gastric cancer, prostate cancer (including prostate adenocarcinoma), endometrial cancer (including uterine carcinosarcoma, endometrial carcinoma), rectal adenocarcinoma, thyroid cancer, colon adenocarcinoma, gastric adenocarcinoma, and pancreatic cancer (including pancreatic adenocarcinoma). In some embodiments, the antibody or antibody drug conjugate of the invention is used in a method for treating NSCLC. In some embodiments, the antibody or antibody drug conjugate of the invention is used in a method for treating head and neck cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating skin cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating esophageal cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating breast cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating ovarian cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating bladder cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating cervical cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating gastric cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating renal cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating endometrial cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating stomach cancer. In some embodiments, the antibody or antibody drug conjugate of the present invention is used in a method for treating pancreatic cancer.The treatment can be applied to patients with primary or metastatic tumors of these types, and also to patients who are refractory to conventional treatments or who have relapsed after responding to such treatments.

[0161] The antibody of the present invention, e.g., humanized antibody, alone or as a conjugate thereof, is administered in an effective dosing regimen, meaning a dosage, route of administration, and frequency of administration that delays onset, reduces severity, inhibits further progression, and / or improves at least one sign or symptom of cancer. If the patient already has cancer, the dosing regimen may be referred to as a therapeutically effective dosing regimen. If the patient is at increased risk of cancer compared to the general population, but has not yet experienced symptoms, the dosing regimen may be referred to as a prophylactically effective dosing regimen. In some cases, a therapeutic or preventive effect may be observed in an individual patient compared to historical controls or past experience in the same patient. In other examples, therapeutic or preventive efficacy may be demonstrated in a population of treated patients compared to a control population of untreated patients in preclinical or clinical trials.

[0162] Exemplary dosages of monoclonal antibodies are from 0.1 mg / kg to 50 mg / kg, more typically from 1 mg / kg to 30 mg / kg, 1 mg / kg to 20 mg / kg, 1 mg / kg to 15 mg / kg, 1 mg / kg to 12 mg / kg, or 1 mg / kg to 10 mg / kg, 1 or 2 mg / kg to 30 mg / kg, 2 mg / kg to 20 mg / kg, 2 mg / kg to 15 mg / kg, 2 mg / kg to 12 mg / kg, or 2 mg / kg to 10 mg / kg, or 3 mg / kg to 30 mg / kg, 3 mg / kg to 20 mg / kg, 3 mg / kg to 15 mg / kg, 3 mg / kg to 12 mg / kg, or 3 mg / kg to 10 mg / kg based on the patient's body weight. Exemplary dosages for monoclonal antibodies or antibody drug conjugates are 1 mg / kg to 7.5 mg / kg, or 2 mg / kg to 7.5 mg / kg, or 3 mg / kg to 7.5 mg / kg, or 0.1 to 20, or 0.5 to 5 mg / kg body weight (e.g., 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 mg / kg), or 10 to 1500 or 200 to 1500 mg as a fixed dose based on the patient's body weight. In some methods, the patient is administered a dosage of at least 1.5 mg / kg, at least 2 mg / kg, or at least 3 mg / kg, administered one or more times every three weeks. Dosage will depend on, among other factors, the frequency of administration, the condition of the patient, and response to prior treatment, if any, whether the treatment is prophylactic or therapeutic, and whether the disease is acute or chronic.

[0163] Administration can be parenteral, intravenous, oral, subcutaneous, intraarterial, intracranial, intrathecal, intraperitoneal, topical, intranasal, or intramuscular. Administration can also be directly localized, for example, within a tumor. Administration into the systemic circulation by intravenous or subcutaneous administration is preferred. Intravenous administration can be by infusion over a period of time, for example, 30-90 minutes, or by a single bolus injection.

[0164] The frequency of administration depends, inter alia, on the half-life of the antibody or conjugate in circulation, the condition of the patient, and the route of administration. The frequency can be daily, weekly, monthly, every three months, or irregular intervals depending on the change in the patient's condition or the progression of the cancer being treated. An example of the frequency of intravenous administration is, for example, twice a week to four times a year over a course of treatment, although more frequent or less frequent administrations are also possible. Another example of the frequency of intravenous administration is, for example, once a week to three times every four weeks over a course of treatment, although more frequent or less frequent administrations are also possible. For subcutaneous administration, the frequency of administration can be, for example, daily to monthly, although more frequent or less frequent administrations are also possible.

[0165] The number of doses will depend on the characteristics of the cancer (e.g., acute or chronic) and the response of the disease to treatment. For acute disease, or acute exacerbations of chronic disease, 1-10 doses are often sufficient. For acute disease, or acute exacerbations of chronic disease, a single bolus dose, or optionally divided doses, may be sufficient. Treatment can be repeated for recurrence of acute disease or acute exacerbations. For chronic disease, the antibody can be administered at regular intervals, e.g., weekly, biweekly, monthly, quarterly, or six monthly, for at least 1, 5, or 10 years, or for the life of the patient.

[0166] Pharmaceutical compositions for parenteral administration are preferably sterile, substantially isotonic and manufactured under GMP conditions. Pharmaceutical compositions can be provided in unit dosage form (i.e., one-time dosage form). Pharmaceutical compositions can be formulated with one or more physiologically acceptable carriers, excipients, additives or adjuvants. The formulation will depend on the route of administration selected. For injection, the antibody can be formulated in an aqueous solution, preferably in a physiologically compatible buffer such as Hank's solution, Ringer's solution, or physiological saline or acetate buffer (to reduce discomfort at the injection site). The solution can contain formulating agents such as suspending, stabilizing and / or dispersing agents. Alternatively, the antibody may be in lyophilized form for constitution before use with a suitable vehicle, e.g., sterile pyrogen-free water. The antibody concentration in the liquid formulation can be, for example, 1-100 mg / ml, e.g., 10 mg / ml.

[0167] Treatment with the antibodies of the invention can be combined with chemotherapy, radiation, stem cell therapy, surgery, or other treatments effective against the disease being treated. Other useful classes of drugs that can be administered with the antibodies and antibody drug conjugates against αvβ6 as described herein include, for example, antibodies against other receptors expressed on cancer cells, antitubulin agents (e.g., auristatins), DNA minor groove binding agents, DNA replication inhibitors, alkylating agents (e.g., platinum complexes, such as cisplatin, mono(platinum), bis(platinum) and trinuclear platinum complexes, and carboplatin), anthracyclines, antibiotics, antifolates, antimetabolites, chemotherapy sensitizers, duocarmycins, etoposides, fluoropyrimidines, ionophores, lexitropsins, nitrosoureas, platinols, pre-forming compounds, purine antimetabolites, puromycins, radiosensitizers, steroids, taxanes, topoisomerase inhibitors, vinca alkaloids, and the like.

[0168] Treatment with an anti-αvβ6 antibody or antibody drug conjugate (optionally in combination with any of the other agents or regimens as described above, either alone or as an antibody drug conjugate) has been shown to improve the progression of tumors (e.g., non-small cell lung cancer (NSCLC) (squamous cell carcinoma and adenocarcinoma), head and neck cancer (including head and neck squamous cell carcinoma), esophageal cancer, breast cancer (including invasive breast cancer), ovarian cancer, bladder cancer (including urothelial carcinoma), skin cancer, and the like) compared to the same treatment (e.g., chemotherapy) but without the anti-αvβ6 antibody (alone or as a conjugate). The median progression-free survival or overall survival of patients with cancer, particularly recurrent or refractory cancers, can be increased by at least 30% or 40%, preferably 50%, 60%-70%, or even 100% or more, of patients with squamous cell carcinoma, or SCC, renal cancer (including clear cell renal cell carcinoma, papillary renal cell carcinoma, chromophobe renal cell carcinoma), cervical cancer, gastric cancer, prostate cancer (including prostate adenocarcinoma), endometrial cancer (including uterine carcinosarcoma, endometrial carcinoma), rectal adenocarcinoma, thyroid cancer, colon adenocarcinoma, gastric adenocarcinoma, and pancreatic cancer (including pancreatic adenocarcinoma). Additionally or alternatively, a treatment (e.g., standard chemotherapy) including an anti-αvβ6 antibody (alone or as a conjugate) can increase the complete response rate, partial response rate, or objective response rate (complete + partial) in tumor-bearing patients by at least 30% or 40%, preferably 50%, 60%-70%, or even 100%, compared to the same treatment (e.g., chemotherapy) but without the anti-αvβ6 antibody (alone or as a conjugate).

[0169] Typically, in a clinical trial (e.g., a Phase II, Phase II / III or Phase III trial), the increase in median progression-free survival and / or response rate of patients treated with standard treatment plus anti-αvβ6 antibody (alone or as a conjugate) relative to a control group of patients receiving standard treatment alone (or plus placebo) is statistically significant, e.g., at p=0.05 or 0.01, or even at the 0.001 level. Complete and partial response rates are determined by objective criteria typically used in cancer clinical trials, e.g., as described or approved by the National Cancer Institute and / or the Food and Drug Administration.

[0170] IX. Products or Kits In another aspect, an article of manufacture or kit is provided that includes the anti-αvβ6 antibody or anti-αvβ6 antibody drug conjugate described herein. The article of manufacture or kit may further include instructions for using the anti-αvβ6 antibody or anti-αvβ6 antibody drug conjugate described herein in the method of the invention. That is, in certain embodiments, the product or kit includes instructions for using an anti-αvβ6 antibody or anti-αvβ6 antibody drug conjugate described herein in a method for treating cancer in a subject (e.g., non-small cell lung cancer (NSCLC) (squamous cell carcinoma and adenocarcinoma), head and neck cancer (including head and neck squamous cell carcinoma), esophageal cancer, breast cancer (including invasive breast carcinoma), ovarian cancer, bladder cancer (including urothelial carcinoma), skin cancer (squamous cell carcinoma, i.e., SCC), renal cancer (including clear cell renal cell carcinoma, papillary renal cell carcinoma, chromophobe renal cell carcinoma), cervical cancer, gastric cancer, prostate cancer (including prostate adenocarcinoma), endometrial cancer (including uterine carcinosarcoma, endometrial carcinoma), rectal adenocarcinoma, thyroid cancer, colon adenocarcinoma, gastric adenocarcinoma, and pancreatic cancer (including pancreatic adenocarcinoma)), comprising administering to the subject an effective amount of an anti-αvβ6 antibody or anti-αvβ6 antibody drug conjugate described herein. In some embodiments, the cancer is NSCLC. In some embodiments, the cancer is head and neck cancer. In some embodiments, the cancer is esophageal cancer. In some embodiments, the cancer is breast cancer. In some embodiments, the cancer is ovarian cancer. In some embodiments, the antibody or antibody drug conjugate of the invention is used in a method of treating renal cancer. In some embodiments, the antibody or antibody drug conjugate of the invention is used in a method of treating endometrial cancer. In some embodiments, the antibody or antibody drug conjugate of the invention is used in a method of treating stomach cancer. In some embodiments, the cancer is bladder cancer. In some embodiments, the cancer is skin cancer. In some embodiments, the cancer is cervical cancer. In some embodiments, the cancer is gastric cancer. In some embodiments, the cancer is pancreatic cancer. In some embodiments, the subject is a human.

[0171] The product or kit may further include a container. Suitable containers include, for example, bottles, vials (e.g., dual chamber vials), syringes (such as single or dual chamber syringes) and test tubes. In some embodiments, the container is a vial. The container can be made of a variety of materials, such as glass or plastic. The container holds the formulation.

[0172] The article of manufacture or kit can further include a label or package insert, which can be on or associated with the container and can indicate instructions for reconstitution and / or use of the formulation. The label or package insert can further indicate that the formulation is useful or intended for subcutaneous administration, intravenous administration, or other modes of administration for the treatment of cancer in a subject, such as non-small cell lung cancer (NSCLC) (squamous cell carcinoma and adenocarcinoma), head and neck cancer (including head and neck squamous cell carcinoma), esophageal cancer, breast cancer (including invasive breast cancer), ovarian cancer, bladder cancer (including urothelial carcinoma), skin cancer (squamous cell carcinoma, or SCC), kidney cancer (including clear cell renal cell carcinoma, papillary renal cell carcinoma, chromophobe renal cell carcinoma), cervical cancer, gastric cancer, prostate cancer (including prostate adenocarcinoma), endometrial cancer (including uterine carcinosarcoma, endometrial carcinoma), rectal adenocarcinoma, thyroid cancer, colon adenocarcinoma, gastric adenocarcinoma, and pancreatic cancer (including pancreatic adenocarcinoma). The container holding the formulation may be a single-use or multi-use vial, which allows for repeated administration of the reconstituted formulation. The article of manufacture or kit may further include a second container containing a suitable diluent. The article of manufacture or kit may further include other materials desirable from a commercial, therapeutic, and user standpoint, including other buffers, diluents, filters, needles, syringes, and package inserts with instructions for use.

[0173] The article of manufacture or kit herein optionally further comprises a container containing a second medicament, and the anti-αvβ6 antibody or anti-αvβ6 antibody drug conjugate is a first medicament, and the article of manufacture or kit further comprises instructions on a label or package insert for treating a subject with an effective amount of the second medicament. In some embodiments, the second medicament is for eliminating or reducing the severity of one or more adverse events.

[0174] In some embodiments, the anti-αvβ6 antibody or anti-αvβ6 antibody drug conjugate is present in a container as a lyophilized powder. In some embodiments, the lyophilized powder is in a sealed container, such as a vial, an ampoule, or a sachette, indicating the amount of active agent. If the medicament is administered by injection, an ampoule of, for example, sterile water for injection or saline can be optionally provided as part of the kit so that the components can be mixed before administration. Such kits can further include one or more of various conventional pharmaceutical components, such as, for example, a container with one or more pharma- ceutically acceptable carriers, additional containers, as needed, and are readily understood by those skilled in the art. Printed instructions as a package insert or label indicating the amount of components to be administered, administration guidelines, and / or mixing guidelines of the components can also be included in the kit.

[0175] X. Other Uses The anti-αvβ6 antibodies described herein, e.g., humanized anti-αvβ6 antibodies, can be used to detect αvβ6 in the context of clinical diagnosis or therapy or research. Expression of αvβ6 in a cancer indicates that the cancer is suitable for treatment with the antibodies of the present invention. The antibodies can also be sold as research reagents for studies on the detection of cells bearing αvβ6 and their responses to various stimuli. In such uses, the monoclonal antibodies can be labeled with fluorescent molecules, spin-labeled molecules, enzymes, or radioisotypes and provided in the form of a kit containing all the reagents necessary to perform an assay for αvβ6. The antibodies described herein can be used to detect expression of αvβ6 protein and determine whether a cancer is suitable for treatment with αvβ6 ADC.

[0176] All patent applications, websites, other publications, accession numbers, etc., listed above or below, are incorporated herein by reference in their entirety for all purposes to the same extent as if each individual item was specifically and individually indicated to be similarly incorporated by reference. Where different versions of sequences are associated with accession numbers at different times, the version associated with the accession number at the effective filing date of this application is meant. The effective filing date means the filing date or, if applicable, the filing date of the priority application that references that accession number, whichever is earlier. Similarly, where different versions of publications, websites, etc. are published at different times, the most recently published version at the time of the effective filing date of this application is meant, unless otherwise indicated. Any feature, step, element, embodiment, or aspect of the present invention may be used in combination with any other, unless specifically stated otherwise. Although the present invention has been described in some detail by way of illustration and example for purposes of clarity and understanding, it will be apparent that certain changes and modifications may be practiced within the scope of the appended claims.

[0177] Working Example material The cell lines described in the following examples were maintained in culture according to the conditions specified by the American Type Culture Collection (ATCC), the National Cancer Institute (NCI), or the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany (DMSZ). The SW780, Detroit 562, HPAFII, and BxPC3 cell lines were obtained from ATCC. FreeStyle™ 293-F (InVitrogen Corp) human epithelial kidney cells and corresponding transfectants were maintained according to the manufacturer's instructions. Cell culture reagents were obtained from Invitrogen Corp. (Carlsbad, CA), Molecular Devices (Sunnydale, CA), and other suppliers. Secondary antibody reagents were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). Recombinant αvβ1, αvβ3, αvβ5, αvβ6, and αvβ8 were purchased from R&D Systems (Minneapolis, MN). FreeStyle™ 293-F cells express endogenous integrin αv and were stably transfected with full-length cDNAs encoding human, cynomolgus monkey, or mouse integrin β6 to generate HEK293F:huβ6, HEK293F:cynoβ6, HEK293F:muβ6 cell lines, respectively. HEK293F cells transfected with empty vector (HEK293F:vector) were used as negative controls. Mouse 3T3 and FDC-P1 cells expressing endogenous mouse integrin αv were transfected with full-length cDNA clones of human and mouse integrin β6 to generate 3T3:huβ6 and FDC-P1:muβ6, respectively.

[0178] Methodology: Generation of 2A2 antibody ICR (CD-1) mice were treated with approximately 5 × 10 6Mice were immunized three times with intraperitoneal injections of 3T3:huβ6 transfectants. Three days prior to fusion, mice received a final injection of purified recombinant human αvβ6 intravenously (6ug) and intraperitoneally (30ug). Lymphocytes harvested from spleens and lymph nodes were fused with P3X63Ag8.653 myeloma cells using polyethylene glycol. Fused cells were allowed to recover overnight in Hybridoma Growth Medium (IMDM containing 4mM glutamine, 10% fetal clone I, 10% cloning factors and penicillin / streptomycin). After recovery, cells were spun down and plated on semi-solid medium. Semi-solid medium consisted of Hybridoma Growth Medium plus CloneMatrix medium supplemented with HAT for hybridoma selection and CloneDetect for IgG production. Hybridomas were incubated at 37°C for 10 days. On day 10, IgG-producing hybridoma clones were harvested using ClonePixFL (Molecular Devices) and transferred to 96-well plates containing IgG-depleted hybridoma growth medium plus HT. Hybridoma culture supernatants were screened with 293F:huβ6 transfectants, and positive clones were identified using Alexifluor-647-labeled secondary antibodies for detection. Plates were read on an FMAT 8200 (Applied Biosystems). Hybridomas that bound to 293F:huβ6 and 293F:cynoβ6, but not to 293F:vector, were expanded for direct conjugation to drug linkers. The directly conjugated antibody panel was tested in binding and cytotoxicity assays.

[0179] LAP shielding ELISA 96-well microtiter plates (Nunc) were coated with 0.3ug / mL recombinant human latency associated peptide (rhuLAP) (in-house; lot 09-19-09DS) in 1xPBS overnight at 4°C. After removing the coating solution, the plates were blocked with 3% BSA in Tris-buffered saline (TBS) for 1 hour at room temperature and then washed 5 times with PBS+0.05% Tween-20 (PBST) before use. In another conical-bottom 96-well plate, 0.25ug / mL recombinant human (rhu)αvβ6-biotin (in-house rhuαvβ6-biotin, lot no. 171030A) was pre-incubated with increasing concentrations of purified antibodies in TBS buffer containing 1mM CaCl2, 1mM MgCl2 and 1mg / mL BSA for 1 hour at room temperature. The antibody / αvβ6-biotin mixture was then transferred to the LAP-coated plate and incubated for 1 hour at room temperature. Plates were washed as above and incubated with 50uL / well of peroxidase-conjugated streptavidin (Jackson ImmunoResearch #016-030-084) diluted 1:1000 in TBS+1mg / mL BSA for 1 hour at room temperature. Bound protein and signal were detected using TMB (Invitrogen #00-2023) incubated for 5.5 minutes and quenched with 1N H2SO4 (Fisher #SA212-1). Plates were immediately read at 450nm wavelength using a plate reader (Molecular Devices Vmax Kinetic Microplate Reader). Data was exported to Microsoft Excel and analyzed using GraphPad Prism v5.03.

[0180] Competitive Binding Assay – Humanized 2A2 Antibody Variants Competitive binding assays were performed using the 293F:huβ6 cell line. 6Antigen-expressing cells were dispensed into each well of a 96-well V-bottom plate on ice. Cells were incubated with 2 nM AlexaFluor-647-labeled m2A2 and increasing concentrations (0.03-500 nM) of unlabeled humanized 2A2 mutant antibodies in FACS buffer (Tris-buffered saline, 2% fetal bovine serum, 0.5 mM MnCl2, 0.02% NaN3) for 1 h. Cells were pelleted and washed three times with TBS / FBS. Cells were pelleted and resuspended in 125 uL of TBS / FBS. Fluorescent signal of binding was detected using a Becton Dickinson Biosciences LSR II (San Jose, CA). The percentage of saturated fluorescent signal was used to determine the percentage of labeled humanized 2A2 antibody bound, and the EC50 was subsequently estimated by fitting the data to a sigmoidal dose-response curve with variable slope using GraphPad software (LaJolla, CA).

[0181] Competitive Binding Assay - Human and Cynomolgus Monkey (cyno) αvβ6 Competitive binding assays were performed using 293F:huβ6 and HEK293F:cynoβ6 cell lines. 6Antigen-expressing cells were dispensed into each well of a 96-well V-bottom plate on ice. Cells were incubated with 2 nM AlexaFluor-647-labeled humanized 2A2 HCLG and increasing concentrations (4 pM to 1 uM) of unlabeled humanized 2A2 HCLG antibody and h2A2-Mdpr-PEG(12)-gluc-MMAE(8) in buffer (Tris-buffered saline, 2% fetal bovine serum, 0.5 mM MnCl2, 0.02% NaN3) for 1 h. Cells were pelleted and washed three times with TBS / FBS. Cells were pelleted and resuspended in 125 uL TBS / FBS. Fluorescent signals of binding were detected using a Becton Dickinson Biosciences LSR II (San Jose, CA). The percentage of saturated fluorescent signal was used to determine the percentage of labeled humanized 2A2 antibody bound to cells, and the EC50 was subsequently estimated by fitting the data to a sigmoidal dose-response curve with variable slope using GraphPad software (LaJolla, CA).

[0182] αvβ6 Saturation Binding Assay - Human and Cynomolgus Monkey (cyno) αvβ6 Saturation binding studies were performed using the following antigen-expressing cell lines: 293F:huβ6 and 293F:cynoβ6. 6 Antigen-expressing cells were dispensed per well into a 96-well V-bottom plate. m2A2 and h2A2 HCLGs were directly labeled with AlexaFluor-647 and added to the cells at concentrations ranging from 6 pM to 340 nM in buffer (Tris-buffered saline, 2% fetal bovine serum, 0.5 mM MnCl2, 0.02% NaN3). Cells were incubated for 1 hour, then pelleted and washed three times with TBS. Cells were pelleted and resuspended in 120 uL of TBS. Fluorescent signals of binding were detected using a Becton Dickinson Biosciences LSR II (San Jose, CA). EC50 was calculated using GraphPad software (LaJolla, CA).

[0183] ELISA 96-well Maxisorb plates (Nunc) were coated overnight at 4°C with recombinant human αvβ1, αvβ3, αvβ5, αvβ6, and αvβ8 (R&D Systems, MN) at 1ug / ml diluted in 50mM carbonate buffer (Sigma, MO). Plates were washed with PBS+0.05% Tween 20 (PBS-T). Wash buffer was removed and plates were blocked in TBS blocking buffer (TBS, 0.05% Tween 20, 1% BSA) for 2 hours at room temperature. Plates were washed and then incubated for 2 hours with humanized 2A2 antibody diluted in TBS binding buffer (TBS, 0.05% Tween 20, 1% BSA, 1mM MnCl2) at concentrations ranging from 1pM to 67nM (10ug / ml). Plates were washed and incubated with a 1:5000 dilution of anti-human Fc-HRP (Jackson ImmunoResearch, PA) for 1 h, washed, and incubated with TMB substrate for 5 min. The reaction was stopped with 1 M HCl. Absorbance was read at 450 nm using a Fusion HT plate reader (Perkin Elmer, Waltham, MA).

[0184] Quantitative flow cytometry analysis Quantification of cell surface αvβ6 copy number was determined using the DAKO QiFiKit flow cytometry indirect assay as described by the manufacturer (DAKO A / S, Glostrup, Denmark) using mouse αvβ6 mAb as the primary antibody and assessed using a Becton Dickinson FACScan flow cytometer.

[0185] In vitro cytotoxicity assay Tumor cells were incubated with anti-αvβ6 antibody drug conjugates for 96 h at 37° C. Cell viability was determined using the CellTiter-Glo® luminescent assay (Promega Corporation, Madison, WI) and results were measured on an EnVision multilabel plate reader (Perkin Elmer, Waltham, MA). Results were expressed as IC, defined as the concentration resulting in half maximum growth inhibition over the course of a titration curve. 50 It is reported as:

[0186] Production of antibody drug conjugates Antibody drug conjugates of anti-αvβ6 antibodies were prepared as described in US Patent Publication No. 20050238649 and WO 2015 / 057699. Both drug linkers vcMMAE (also known as 1006) and mcMMAF are described in US Patent Publication No. 20050238649, which is incorporated herein by reference for all purposes. The drug linker MDpr-Lys(PEGx)-glucuronide-MMAE linker is described in WO 2015 / 057699, which is incorporated herein by reference for all purposes.

[0187] In vivo activity studies For therapeutic experiments in cell line-derived xenografts, 5x10 for studies of BxPC3, Detroit 562, HPAF-II, and SW780 6 Cells (ATCC) were injected subcutaneously into 5–8 female nude (nu / nu) mice (Envigo). Mice were randomly assigned to study groups and tumors were grown until approximately 100 mm 3 When the tumor volume reached 500-1000 mm, the test product was administered by intraperitoneal injection. 3Animals were euthanized when tumor volume reached 100%. Tumor volume was calculated using the formula (volume = 1 / 2 × length × width × width). Mice that showed durable regression were sacrificed around days 40–65 post-implantation. In all xenograft studies, no weight loss or treatment-related toxicity was observed in mice treated with any of the test articles. All animal procedures were performed in facilities accredited by the Association for Assessment and Accreditation of Laboratory Animal Care and under protocols approved by the Institutional Animal Care and Use Committee.

[0188] In addition to cell line-derived xenografts, the antitumor activity of the h2A2 vcMMAE ADC against patient-derived xenograft (PDX) models of non-small cell lung cancer (NSCLC) was studied using models maintained by Champions Oncology (Hackensack NJ). These PDX models contain NSCLC samples of both glandular and squamous histology. These models were established by implantation into immunocompromised mice and ranged from 150–300 mm 3 After allowing tumors to grow to a volume of 1500 mm, mice were randomized into treatment and control groups and administered h2A2 vcMMAE or non-binding control h00 vcMMAE ADC. Mice were dosed weekly with 3 mg / kg of ADC for a total of three doses. Tumor volumes were monitored for 28 days after the first dose or when tumors reached 1500 mm. 3 The volume was measured twice a week until the volume reached 100 mg / kg.

[0189] The antitumor activity of the h2A2 vcMMAE ADC was further evaluated in PDX models of ovarian cancer maintained by Champions Oncology (Hackensack NJ). These models were established by implantation into immunocompromised mice and consisted of 150–300 mm 3After allowing tumors to grow to a volume of 1500 mm, mice were randomized into treatment and control groups and administered ADC. Mice were dosed weekly with 5 mg / kg ADC for a total of three doses. Tumor volumes were monitored for 28 days after the first dose or until tumors reached 1500 mm. 3 Measurements were taken twice weekly for up to 60 days until a volume of 100 μg / kg was reached.

[0190] result The murine clone m2A2 was selected from the hybridoma panel because it showed cytotoxic activity as an ADC in multiple αvβ6-positive tumor cell lines and had comparable affinity for the human and cynomolgus forms of the antigen. The specificity of murine 2A2 was confirmed by FMAT and flow cytometry binding studies that showed that the antibody bound to 293F:huβ6 transfectants but not to the αvβ5-positive parental line (293F:vector). Integrin αvβ6 has been shown to be a receptor for the RGD site of fibronectin (Weinacker et al., 1994), tenascin (Prieto et al., 1993), vitronectin (Huang et al., 1998), and latency-associated peptide (LAP) (Munger et al., 1999). Binding of integrin αvβ6 to LAP can induce spatially restricted activation of transforming growth factor beta 1 (TGFβ) (Munger et al., 1999). A latency-associated peptide (LAP) blocking assay (Figure 1) was performed, showing that m2A2 (SG-44.2A2) did not block LAP, in contrast to the anti-αvβ6 antibody m15H3 (SG-42.15H3) (see WO 2013 / 123152) and the positive control 10D5, suggesting that the 2A2 antibody is delivered independently of ligand binding. Negative controls were a non-binding IgG control, and SG-44.8B9, SG-33.20B8, SG-44.32A6, and SG-44.34D6 were other clones selected from the hybridoma panel.

[0191] Mouse antibody binding The EC50 for binding of mouse monoclonal antibody 2A2 was determined for human and cynomolgus monkey (cyno) αvβ6 by saturation binding studies using engineered cell lines (293F:huβ6, 293F:cynoβ6) (Figure 2). The engineered cell lines express endogenous αv paired with recombinant β6 chains, producing a heterodimeric receptor composed of endogenous αv and recombinant β6.

[0192] Humanized antibody design and selection The starting point or donor antibody for humanization in this example is the murine 2A2 antibody. Genomic sequences provided by IGHV1-46 and IGHJ4 for the heavy chain and IGKV1D-33 and IGKJ2 for the light chain were used.

[0193] For humanization, ten positions were identified in the heavy chain framework (H2, H28, H48, H67, H69, H71, H73, H74, H78, H93; Figure 3) and two positions in the light chain framework (L69 and L71; Figure 4) where the human acceptor sequence differs from the donor sequence and may directly contact the antigen, affect the conformation of the CDRs, or affect the packing between the heavy and light chains, thereby affecting antibody binding. Four humanized heavy chain variants (vHA, vHB, vHC, vHD; Figure 3) and eight humanized light chain variants (vLA, vLB, vLC, vLD, vLE, vLF, vLG, vLH; Figure 4) were created by incorporating backmutations at different permutations of these positions. These backmutations are specified in Tables 1-4. The remaining positions in the framework are occupied by residues from the human acceptor sequence.

[0194] [Table 1]

[0195] [Table 2]

[0196] [Table 3]

[0197] [Table 4]

[0198] Humanized antibodies representing combinatorial permutations of these humanized heavy and light chain variants were then expressed. Competitive binding curves of the resulting humanized antibody variants (along with the murine 2A2 antibody and the human-mouse chimeric antibody) to human αvβ6 are shown in Figures 5-7. Four humanized variants were selected for further analysis in in vitro activity assays. Humanized variants HCLE, HCLG, HCLH, HALG, and humanized anti-αvβ6 antibody 15H3-HTLC (each conjugated to a drug linker; Via, n=12, R PR =H, R 21 The in vitro anticancer activity of αvβ6 (=CH3) was measured using a cytotoxicity assay in four cell lines (pancreatic cancer HPAFII and BxPC-3 cells, head and neck cancer Detroit 562 cells, and bladder cancer SW780 cells) expressing various levels of αvβ6 based on quantitative flow cytometry analysis. The results are shown in Table 5.

[0199] [Table 5]

[0200] Humanized variants including the heavy chain variant hvHC and the light chain variant hvLG (h2A2 HCLG) were selected for further studies.

[0201] Binding of h2A2 HCLG to both human and cynomolgus monkey (cyno) αvβ6 was confirmed by saturation binding (Figure 8) including parental mouse 2A2 as a reference, demonstrating comparable binding with the humanized variants. Binding of h2A2 HCLG to both human and cynomolgus monkey (cyno) αvβ6 was also confirmed by competitive binding to fluorescently labeled mouse 2A2 (Figure 9). The assay also included conjugation of the ADC prepared with h2A2 HCLG to the SGD-5088 drug linker by reducing the interchain disulfide of the antibody, and conjugation with 8 copies of the drug linker. The conjugation process did not affect binding to αvβ6 from either human or cynomolgus monkey (cyno). The binding specificity of h2A2 HCLG was also confirmed by ELISA, where the antibody bound to recombinant human αvβ6 but not αvβ1, αvβ3, αvβ5, or αvβ8 (FIG. 10).

[0202] In vitro anticancer activity of h2A2 ADC The in vitro anticancer activity of humanized mutant HCLG when conjugated to vcMMAE was measured using cytotoxicity assays in four cell lines expressing αvβ6 at various levels (pancreatic cancer HPAFII and BxPC-3 cells, head and neck cancer Detroit 562 cells, and bladder cancer SW780 cells) based on quantitative flow cytometry analysis. Figure 11 shows that the humanized 2A2 anti-αvβ6 ADC exhibited cell killing behavior in these assays.

[0203] In vivo anticancer activity of h2A2 ADC Using the same four cell lines tested in vitro, antitumor activity of humanized 2A2 HCLG antibody conjugated with vcMMAE (average of four drugs per antibody) was demonstrated in vivo (Figures 12-15). Significant tumor growth delay or tumor regression was observed compared to untreated and non-conjugated control ADC. h2A2 HCLG-1006(4) refers to an antibody drug conjugate of an HCLG-humanized form of the parent murine antibody 2A2 with an average of four vcMMAE drug linker molecules per antibody. h00-1006(4) refers to an antibody drug conjugate of a non-conjugated control antibody with an average of four vcMMAE drug linker molecules per antibody.

[0204] In PDX models of NSCLC, humanized 2A2 HCLG antibodies conjugated with vcMMAE (average of four drugs per antibody) also demonstrated antitumor activity (Figure 16). Significant tumor growth delay or tumor regression was observed compared to untreated and non-binding control ADCs. h2A2 HCLG-1006(4) refers to an antibody drug conjugate of the HCLG-humanized form of the parent murine antibody 2A2 with an average of four vcMMAE drug linker molecules per antibody. h00-1006(4) refers to an antibody drug conjugate of the non-binding control antibody with an average of four vcMMAE drug linker molecules per antibody.

[0205] In PDX models of ovarian cancer, humanized 2A2 HCLG antibodies conjugated with vcMMAE (an average of four drugs per antibody) also demonstrated antitumor activity (Figure 17). Significant tumor growth delay or slight tumor reduction was observed compared to untreated tumors. h2A2 HCLG-1006 (4) refers to an antibody drug conjugate of the HCLG-humanized form of the parent murine antibody 2A2 with an average of four vcMMAE drug linker molecules per antibody.

[0206] The antitumor activity of h2A2 HCLG was evaluated using drug-linker VIa with a DAR of 8 (n=12, R PR =H, R 21=CH3). The study protocol was the same as described above for the cell line-derived xenograft model using HPAFII and BxPC3 cell lines. Animals were dosed once with either h2A2 HCLG, h15H3, or non-targeted control (h00) ADC at 3 mg / kg (Figure 18). In both models, the h2A2 HCLG ADC showed durable regression of implanted tumors, whereas the h15H3 ADC showed growth retardation. The present invention includes, for example, the following embodiments: [Embodiment 1] An isolated anti-αvβ6 antibody or antigen-binding fragment thereof comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region is (i) CDR-H1 comprising the amino acid sequence of SEQ ID NO: 31; (ii) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 32, and (iii) CDR-H3 comprising the amino acid sequence of SEQ ID NO: 33 Includes; The light chain variable region is (i) CDR-L1 comprising the amino acid sequence of SEQ ID NO: 37; (ii) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 42, and (iii) CDR-L3 comprising the amino acid sequence of SEQ ID NO: 39 and wherein the CDRs are determined by Kabat, or an antigen-binding fragment thereof. [Embodiment 2] An isolated anti-αvβ6 antibody or antigen-binding fragment thereof comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region is (i) CDR-H1 comprising the amino acid sequence of SEQ ID NO: 34; (ii) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 35, and (iii) CDR-H3 comprising the amino acid sequence of SEQ ID NO: 36 Including, The light chain variable region is (i) CDR-L1 comprising the amino acid sequence of SEQ ID NO: 43; (ii) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 44, and (iii) CDR-L3 comprising the amino acid sequence of SEQ ID NO: 45 and wherein the CDRs are determined by IMGT. [Embodiment 3] The antibody or antigen-binding fragment of embodiment 1 or embodiment 2, wherein the antibody is humanized. [Embodiment 4] An antibody or antigen-binding fragment described in any one of embodiments 1 to 3, wherein the heavy chain variable region comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 6, and the light chain variable region comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 17. [Embodiment 5] An antibody or antigen-binding fragment described in any one of embodiments 1 to 4, wherein the heavy chain variable region comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 6, and the light chain variable region comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 17. [Embodiment 6] An antibody or antigen-binding fragment described in any one of embodiments 1 to 5, wherein the heavy chain variable region comprises an amino acid sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 6, and the light chain variable region comprises an amino acid sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 17. [Embodiment 7] An antibody or antigen-binding fragment described in any one of embodiments 1 to 6, wherein the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 6 and the light chain variable region comprises the amino acid sequence of SEQ ID NO: 17. [Embodiment 8] An antibody or antigen-binding fragment described in any of embodiments 4 to 7, wherein H2 is occupied by F, H28 is occupied by S, H48 is occupied by I, H67 is occupied by A, H69 is occupied by L, H71 is occupied by V, H73 is occupied by K, H78 is occupied by A, H93 is occupied by T, L69 is occupied by R, and L71 is occupied by Y, and the numbering is according to the Kabat numbering system. [Embodiment 9] An antibody or antigen-binding fragment described in any of embodiments 1 to 8, wherein the heavy chain has an amino acid sequence including SEQ ID NO: 21 and the light chain has an amino acid sequence including SEQ ID NO: 29. [Embodiment 10] An antibody or antigen-binding fragment described in any one of embodiments 1 to 9, wherein the antibody or antigen-binding fragment is an antigen-binding fragment selected from the group consisting of Fab, Fab', F(ab')2, Fab'-SH, Fv, diabody, linear antibody, and single-chain antibody fragment. [Embodiment 11] An antibody or antigen-binding fragment described in any one of embodiments 1 to 10, in which the heavy chain variable region of the antibody is fused to a heavy chain constant region and the light chain variable region is fused to a light chain constant region. [Embodiment 12] An antibody or antigen-binding fragment described in embodiment 11, wherein the heavy chain constant region is of the IgG1 isotype. [Embodiment 13] An antibody-drug conjugate comprising an antibody or antigen-binding fragment of any one of embodiments 1 to 12 conjugated to a cytotoxic or cytostatic agent. [Embodiment 14] The antibody-drug conjugate of embodiment 13, wherein the antibody or antigen-binding fragment is conjugated to a cytotoxic or cytostatic agent via a linker. [Embodiment 15] An antibody-drug conjugate described in embodiment 13 or 14, wherein the cytotoxic or cytostatic agent is monomethyl auristatin. [Embodiment 16] The antibody-drug conjugate of any one of embodiments 13 to 15, wherein the monomethylauristatin is monomethylauristatin E (MMAE). [Embodiment 17] The antibody-drug conjugate of embodiment 16, wherein the antibody or antigen-binding fragment thereof is conjugated to MMAE via an enzyme-cleavable linker unit. [Embodiment 18] The antibody-drug conjugate of embodiment 17, wherein the enzyme-cleavable linker unit comprises a Val-Cit linker. [Embodiment 19] The antibody or antigen-binding fragment thereof is represented by the formula: -A a -W w -Y ywherein -A- is a Stretcher unit, a is 0 or 1, -W- is an amino acid unit, w is an integer ranging from 0 to 12, and -Y- is a Spacer unit and y is 0, 1, or 2. [Embodiment 20] The antibody drug conjugate of embodiment 19, wherein the Stretcher unit has the structure of the following formula I, the amino acid unit is Val-Cit; and the Spacer unit is a p-aminobenzyl alcohol (PAB) group having the structure of the following formula II. [ka]

[0041]

[0042] [Embodiment 21] A linker is attached to monomethyl auristatin E and has the following structure: [ka] (wherein Ab is the antibody h2A2 and p is a number from 1 to 16). 21. The antibody drug conjugate of any of embodiments 14 to 20, forming an antibody drug conjugate having the formula: [Embodiment 22] The antibody-drug conjugate of embodiment 21, wherein the average value of p in a population of antibody-drug conjugates is about 4. [Embodiment 23] A nucleic acid encoding a heavy chain variable region and / or a light chain variable region defined by any one of embodiments 1 to 12. [Embodiment 24] A vector comprising the nucleic acid described in embodiment 23. [Embodiment 25] A vector described in embodiment 24, which is an expression vector. [Embodiment 26] A host cell comprising the nucleic acid described in embodiment 25. [Embodiment 27] The host cell described in embodiment 26, which is a Chinese hamster ovary (CHO) cell. [Embodiment 28] A method for producing an anti-αvβ6 antibody or an antigen-binding fragment thereof, comprising culturing a host cell described in embodiment 26 or 27 under conditions suitable for the production of an anti-αvβ6 antibody or an antigen-binding fragment thereof. [Embodiment 29] The method described in embodiment 28, further comprising isolating the anti-αvβ6 antibody or antigen-binding fragment thereof produced by the host cell. [Embodiment 30] A method for producing an anti-αvβ6 antibody-drug conjugate, comprising culturing a host cell described in embodiment 26 or 27 under conditions suitable for the production of an anti-αvβ6 antibody, isolating the anti-αvβ6 antibody produced from the host cell, and conjugating the anti-αvβ6 antibody to a cytotoxic agent or a cell proliferation inhibitor. [Embodiment 31] The method described in embodiment 30, wherein the anti-αvβ6 antibody is conjugated to a cytotoxic or cytostatic agent via a linker. [Embodiment 32] The method of embodiment 31, wherein the linker is a Val-Cit linker. [Embodiment 33] The method of any one of embodiments 30 to 32, wherein the cytotoxic or cytostatic agent is monomethylauristatin E (MMAE). [Embodiment 34] A method for treating cancer in a subject, comprising administering to the subject an antibody or antigen-binding fragment described in any one of embodiments 1 to 12, or an antibody-drug conjugate described in any one of embodiments 13 to 22. [Embodiment 35] The method described in embodiment 34, wherein the cancer is selected from the group consisting of non-small cell lung cancer (NSCLC), head and neck cancer, esophageal cancer, breast cancer, ovarian cancer, bladder cancer, skin cancer (SCC), ovarian cancer, cervical cancer, gastric cancer, and pancreatic cancer. [Embodiment 36] The method of embodiment 34, wherein the lung cancer is non-small cell lung cancer. [Embodiment 37] The method of embodiment 34, wherein the cancer is head and neck cancer. [Embodiment 38] The method of any of embodiments 34 to 37, wherein the antibody or antigen-binding fragment, or antibody-drug conjugate is in a pharmaceutical composition comprising the antibody or antigen-binding fragment, or antibody-drug conjugate, and a pharma- ceutically acceptable carrier. [Embodiment 39] The method of any one of embodiments 34 to 38, wherein the subject is a human. [Embodiment 40] A pharmaceutical composition comprising an antibody or antigen-binding fragment described in any one of embodiments 1 to 12, or an antibody-drug conjugate described in any one of embodiments 13 to 22, and one or more agents selected from the group consisting of physiologically acceptable carriers, diluents, excipients and adjuvants. [Embodiment 41] The pharmaceutical composition described in embodiment 40, which is administered in combination with radiation or a chemotherapy agent. [Embodiment 42] An antibody drug conjugate comprising an isolated anti-αvβ6 antibody conjugated to vcMMAE, wherein the antibody has a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:6 and a light chain variable region comprising the amino acid sequence of SEQ ID NO:17, and the antibody drug conjugate has the following structure: [ka] (wherein Ab is an antibody and p is a number from 1 to 16). 1. An antibody-drug conjugate comprising:

[0207] array SEQ ID NO:1 - m2A2 vH TIFF2024170517000038.tif13167 SEQ ID NO:2 - mIGHV1-39 (closest related mouse germline V gene) TIFF2024170517000039.tif12167 SEQ ID NO:3 - hIGHV1-46 / HJ4 TIFF2024170517000040.tif12167 SEQ ID NO:4 - h2A2 vHA TIFF2024170517000041.tif16168 SEQ ID NO:5 - h2A2 vHB TIFF2024170517000042.tif16168 SEQ ID NO:6 - h2A2 vHC TIFF2024170517000043.tif16168 sequence number 7 - h2A2 vHD TIFF2024170517000044.tif16168 SEQ ID NO:8 - m2A2 vL TIFF2024170517000045.tif11168 SEQ ID NO:9 - mIGKV12-89 (closest related mouse germline V gene) TIFF2024170517000046.tif11166 SEQ ID NO: 10 - hIGKV1D-33 / KJ2 TIFF2024170517000047.tif11166

[0208] SEQ ID NO:11 - h2A2 vLA TIFF2024170517000048.tif11166 SEQ ID NO:12 - h2A2 vLB TIFF2024170517000049.tif11166 SEQ ID NO:13 - h2A2 vLC TIFF2024170517000050.tif11166 SEQ ID NO:14 - h2A2 vLD TIFF2024170517000051.tif11166 SEQ ID NO:15 - h2A2 vLE TIFF2024170517000052.tif11166 SEQ ID NO:16 - h2A2 vLF TIFF2024170517000053.tif11166 SEQ ID NO:17 - h2A2 vLG TIFF2024170517000054.tif11166 SEQ ID NO:18 - h2A2 vLH TIFF2024170517000055.tif11166 SEQ ID NO:19 - h2A2 HA heavy chain TIFF2024170517000056.tif41168 SEQ ID NO:20 - h2A2 HB heavy chain TIFF2024170517000057.tif41168

[0209] SEQ ID NO:21 - h2A2 HC heavy chain TIFF2024170517000058.tif41168 SEQ ID NO:22 - h2A2 HD heavy chain TIFF2024170517000059.tif41168 SEQ ID NO:23 - h2A2 LA light chain TIFF2024170517000060.tif21165 SEQ ID NO:24 - h2A2 LB light chain TIFF2024170517000061.tif22167 SEQ ID NO:25 - h2A2 LC light chain TIFF2024170517000062.tif22166 SEQ ID NO:26 - h2A2 LD light chain TIFF2024170517000063.tif22166 SEQ ID NO:27 - h2A2 LE light chain TIFF2024170517000064.tif22166 SEQ ID NO:28 - h2A2 LF light chain TIFF2024170517000065.tif22166 SEQ ID NO:29 - h2A2 LG light chain TIFF2024170517000066.tif22166 SEQ ID NO:30 - h2A2 LH light chain TIFF2024170517000067.tif22167

[0210] SEQ ID NO:31 - HA, HB, HC, HD CDR1, KABAT DYNVN SEQ ID NO:32 - HA, HB, HC, HD CDR2, KABAT VINPKYGTTRYNQKFKG SEQ ID NO:33 - HA, HB, HC, HD CDR3, KABAT GLNAWDY SEQ ID NO:34 - HA, HB, HC, HD CDR1, IMGT GYSFTDYN SEQ ID NO:35 - HA, HB, HC, HD CDR2, IMGT INPKYGTT SEQ ID NO:36 - HA, HB, HC, HD CDR3, IMGT TRGLNAWDY SEQ ID NO:37 - LA, LB, LC, LD, LG, LH CDR1, KABAT GASENIYGALN SEQ ID NO:38 - LA, LB, LD, LE, LH CDR2, KABAT GATNLAD SEQ ID NO:39 - LA, LB, LC, LD, LE, LF, LG, LH CDR3, KABAT QNVLTTPYT SEQ ID NO:40 - LE, LF CDR1, KABAT QASENIYGALN

[0211] SEQ ID NO:41 - LC, LF CDR2, KABAT GATNLAT SEQ ID NO:42 - LG CDR2, KABAT GATNLED SEQ ID NO: 43 - LA, LB, LC, LD, LE, LF, LG, LH CDR1, IMGT ENIYGA SEQ ID NO:44 - LA, LB, LC, LD, LE, LF, LG, LH CDR2, IMGT GAT SEQ ID NO: 45 - LA, LB, LC, LD, LE, LF, LG, LH CDR3, IMGT QNVLTTPYT

[0212] [Sequence table] SEQUENCE LISTING <110> Seagen Inc. <120> ANTI- AVB6 ANTIBODIES AND ANTIBODY-DRUG CONJUGATES <130> PA24-463 <150> US 62 / 943,959 <151> 2019-12-05 <150> US 63 / 012,584 <151> 2020-04-20 <160> 45 <170> PatentIn version 3.5 <210> 1 <211> 116 <212> PRT <213> Artificial Sequence <220> <223> m2A2 vH <400> 1 Glu Phe Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 20 25 30 Asn Val Asn Trp Val Lys Gln Ser Asn Gly Lys Ser Leu Glu Trp Ile 35 40 45 Gly Val Ile Asn Pro Lys Tyr Gly Thr Thr Arg Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Pro Ser Ser Thr Ala Tyr 65 70 75 80 Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 85 90 95 Thr Arg Gly Leu Asn Ala Trp Asp Tyr Trp Gly Gln Gly Ala Ser Val 100 105 110 Thr Val Ser Ser 115 <210> 2 <211> 98 <212> PRT <213> Artificial Sequence <220> <223> mIGHV1-39 (closest murine germline V-gene) <400> 2 Glu Phe Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 20 25 30 Asn Met Asn Trp Val Lys Gln Ser Asn Gly Lys Ser Leu Glu Trp Ile 35 40 45 Gly Val Ile Asn Pro Asn Tyr Gly Thr Thr Ser Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Lys Ala Thr Leu Thr Val Asp Gln Ser Ser Ser Thr Ala Tyr 65 70 75 80 Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 85 90 95 Ala Arg <210> 3 <211> 113 <212> PRT <213> Artificial Sequence <220> <223> hIGHV1-46 / HJ4 <400> 3 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 20 25 30 Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Ile Ile Asn Pro Ser Gly Gly Ser Thr Ser Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 100 105 110 Ser <210> 4 <211> 116 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vHA <400> 4 Gln Phe Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 20 25 30 Asn Val Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Val Ile Asn Pro Lys Tyr Gly Thr Thr Arg Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Thr Arg Gly Leu Asn Ala Trp Asp Tyr Trp Gly Gln Gly Thr Leu Val 100 105 110 Thr Val Ser Ser 115 <210> 5 <211> 116 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vHB <400> 5 Gln Phe Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 20 25 30 Asn Val Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Val Ile Asn Pro Lys Tyr Gly Thr Thr Arg Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Arg Val Thr Met Thr Arg Asp Thr Pro Thr Ser Thr Val Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Thr Arg Gly Leu Asn Ala Trp Asp Tyr Trp Gly Gln Gly Thr Leu Val 100 105 110 Thr Val Ser Ser 115 <210> 6 <211> 116 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vHC <400> 6 Gln Phe Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 20 25 30 Asn Val Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 35 40 45 Gly Val Ile Asn Pro Lys Tyr Gly Thr Thr Arg Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Arg Ala Thr Leu Thr Val Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Thr Arg Gly Leu Asn Ala Trp Asp Tyr Trp Gly Gln Gly Thr Leu Val 100 105 110 Thr Val Ser Ser 115 <210> 7 <211> 116 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vHD <400> 7 Gln Phe Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 20 25 30 Asn Val Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 35 40 45 Gly Val Ile Asn Pro Lys Tyr Gly Thr Thr Arg Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Arg Ala Thr Leu Thr Val Asp Lys Pro Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Thr Arg Gly Leu Asn Ala Trp Asp Tyr Trp Gly Gln Gly Thr Leu Val 100 105 110 Thr Val Ser Ser 115 <210> 8 <211> 107 <212> PRT <213> Artificial Sequence <220> <223> m2A2 vL <400> 8 Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Glu Thr Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Arg Lys Gln Gly Lys Ser Pro Gln Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Met Ser Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Gln Tyr Ser Phe Lys Ile Ser Ser Leu His Pro 65 70 75 80 Asp Asp Val Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 100 105 <210> 9 <211> 95 <212> PRT <213> Artificial Sequence <220> <223> mIGKV12-89 (closest murine germline V-gene) <400> 9 Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Glu Thr Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Arg Lys Gln Gly Lys Ser Pro Gln Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Met Ser Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Gln Tyr Ser Leu Lys Ile Ser Ser Leu His Pro 65 70 75 80 Asp Asp Val Ala Thr Tyr Tyr Cys Gln Asn Val Leu Ser Thr Pro 85 90 95 <210> 10 <211> 107 <212> PRT <213> Artificial Sequence <220> <223> hIGKV1D-33 / KJ2 <400> 10 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Asp Ile Ser Asn Tyr 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Asp Ala Ser Asn Leu Glu Thr Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Asp Asn Leu Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 <210> 11 <211> 107 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vLA <400> 11 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 <210> 12 <211> 107 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vLB <400> 12 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 <210> 13 <211> 107 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vLC <400> 13 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Thr Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 <210> 14 <211> 107 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vLD <400> 14 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 <210> 15 <211> 107 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vLE <400> 15 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 <210> 16 <211> 107 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vLF <400> 16 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Thr Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 <210> 17 <211> 107 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vLG <400> 17 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Glu Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 <210> 18 <211> 107 <212> PRT <213> Artificial Sequence <220> <223> h2A2 vLH <400> 18 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 <210> 19 <211> 446 <212> PRT <213> Artificial Sequence <220> <223> h2A2 HA heavy chain <400> 19 Gln Phe Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 20 25 30 Asn Val Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Val Ile Asn Pro Lys Tyr Gly Thr Thr Arg Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Thr Arg Gly Leu Asn Ala Trp Asp Tyr Trp Gly Gln Gly Thr Leu Val 100 105 110 Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 115 120 125 Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 130 135 140 Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 145 150 155 160 Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 165 170 175 Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 180 185 190 Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 195 200 205 Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 210 215 220 Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 225 230 235 240 Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 245 250 255 Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 260 265 270 Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 275 280 285 Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 290 295 300 Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 305 310 315 320 Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 325 330 335 Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 340 345 350 Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 355 360 365 Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 370 375 380 Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 385 390 395 400 Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 405 410 415 Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 420 425 430 Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 435 440 445 <210> 20 <211> 446 <212> PRT <213> Artificial Sequence <220> <223> h2A2 HB heavy chain <400> 20 Gln Phe Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 20 25 30 Asn Val Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Val Ile Asn Pro Lys Tyr Gly Thr Thr Arg Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Arg Val Thr Met Thr Arg Asp Thr Pro Thr Ser Thr Val Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Thr Arg Gly Leu Asn Ala Trp Asp Tyr Trp Gly Gln Gly Thr Leu Val 100 105 110 Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 115 120 125 Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 130 135 140 Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 145 150 155 160 Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 165 170 175 Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 180 185 190 Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 195 200 205 Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 210 215 220 Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 225 230 235 240 Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 245 250 255 Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 260 265 270 Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 275 280 285 Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 290 295 300 Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 305 310 315 320 Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 325 330 335 Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 340 345 350 Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 355 360 365 Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 370 375 380 Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 385 390 395 400 Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 405 410 415 Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 420 425 430 Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 435 440 445 <210> 21 <211> 446 <212> PRT <213> Artificial Sequence <220> <223> h2A2 HC heavy chain <400> 21 Gln Phe Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 20 25 30 Asn Val Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 35 40 45 Gly Val Ile Asn Pro Lys Tyr Gly Thr Thr Arg Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Arg Ala Thr Leu Thr Val Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Thr Arg Gly Leu Asn Ala Trp Asp Tyr Trp Gly Gln Gly Thr Leu Val 100 105 110 Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 115 120 125 Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 130 135 140 Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 145 150 155 160 Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 165 170 175 Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 180 185 190 Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 195 200 205 Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 210 215 220 Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 225 230 235 240 Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 245 250 255 Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 260 265 270 Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 275 280 285 Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 290 295 300 Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 305 310 315 320 Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 325 330 335 Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 340 345 350 Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 355 360 365 Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 370 375 380 Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 385 390 395 400 Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 405 410 415 Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 420 425 430 Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 435 440 445 <210> 22 <211> 446 <212> PRT <213> Artificial Sequence <220> <223> h2A2 HD heavy chain <400> 22 Gln Phe Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 20 25 30 Asn Val Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 35 40 45 Gly Val Ile Asn Pro Lys Tyr Gly Thr Thr Arg Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Arg Ala Thr Leu Thr Val Asp Lys Pro Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Thr Arg Gly Leu Asn Ala Trp Asp Tyr Trp Gly Gln Gly Thr Leu Val 100 105 110 Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 115 120 125 Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 130 135 140 Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 145 150 155 160 Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 165 170 175 Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 180 185 190 Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 195 200 205 Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 210 215 220 Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 225 230 235 240 Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 245 250 255 Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 260 265 270 Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 275 280 285 Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 290 295 300 Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 305 310 315 320 Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 325 330 335 Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 340 345 350 Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 355 360 365 Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 370 375 380 Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 385 390 395 400 Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 405 410 415 Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 420 425 430 Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 435 440 445 <210> 23 <211> 214 <212> PRT <213> Artificial Sequence <220> <223> h2A2 LA light chain <400> 23 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 100 105 110 Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 115 120 125 Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 130 135 140 Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 145 150 155 160 Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 165 170 175 Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 180 185 190 Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 195 200 205 Phe Asn Arg Gly Glu Cys 210 <210> 24 <211> 214 <212> PRT <213> Artificial Sequence <220> <223> h2A2 LB light chain <400> 24 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 100 105 110 Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 115 120 125 Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 130 135 140 Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 145 150 155 160 Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 165 170 175 Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 180 185 190 Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 195 200 205 Phe Asn Arg Gly Glu Cys 210 <210> 25 <211> 214 <212> PRT <213> Artificial Sequence <220> <223> h2A2 LC light chain <400> 25 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Thr Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 100 105 110 Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 115 120 125 Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 130 135 140 Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 145 150 155 160 Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 165 170 175 Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 180 185 190 Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 195 200 205 Phe Asn Arg Gly Glu Cys 210 <210> 26 <211> 214 <212> PRT <213> Artificial Sequence <220> <223> h2A2 LD light chain <400> 26 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 100 105 110 Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 115 120 125 Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 130 135 140 Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 145 150 155 160 Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 165 170 175 Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 180 185 190 Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 195 200 205 Phe Asn Arg Gly Glu Cys 210 <210> 27 <211> 214 <212> PRT <213> Artificial Sequence <220> <223> h2A2 LE light chain <400> 27 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 100 105 110 Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 115 120 125 Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 130 135 140 Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 145 150 155 160 Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 165 170 175 Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 180 185 190 Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 195 200 205 Phe Asn Arg Gly Glu Cys 210 <210> 28 <211> 214 <212> PRT <213> Artificial Sequence <220> <223> h2A2 LF light chain <400> 28 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Thr Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 100 105 110 Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 115 120 125 Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 130 135 140 Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 145 150 155 160 Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 165 170 175 Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 180 185 190 Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 195 200 205 Phe Asn Arg Gly Glu Cys 210 <210> 29 <211> 214 <212> PRT <213> Artificial Sequence <220> <223> h2A2 LG light chain <400> 29 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Glu Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 100 105 110 Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 115 120 125 Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 130 135 140 Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 145 150 155 160 Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 165 170 175 Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 180 185 190 Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 195 200 205 Phe Asn Arg Gly Glu Cys 210 <210> 30 <211> 214 <212> PRT <213> Artificial Sequence <220> <223> h2A2 LH light chain <400> 30 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile Tyr Gly Ala 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Gly Ala Thr Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Arg Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Asn Val Leu Thr Thr Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 100 105 110 Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 115 120 125 Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 130 135 140 Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 145 150 155 160 Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 165 170 175 Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 180 185 190 Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 195 200 205 Phe Asn Arg Gly Glu Cys 210 <210> 31 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> HA, HB, HC, HD CDR1, KABAT <400> 31 Asp Tyr Asn Val Asn 1 5 <210> 32 <211> 17 <212> PRT <213> Artificial Sequence <220> <223> HA, HB, HC, HD CDR2, KABAT <400> 32 Val Ile Asn Pro Lys Tyr Gly Thr Thr Arg Tyr Asn Gln Lys Phe Lys 1 5 10 15 Gly <210> 33 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> HA, HB, HC, HD CDR3, KABAT <400> 33 Gly Leu Asn Ala Trp Asp Tyr 1 5 <210> 34 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> HA, HB, HC, HD CDR1, IMGT <400> 34 Gly Tyr Ser Phe Thr Asp Tyr Asn 1 5 <210> 35 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> HA, HB, HC, HD CDR2, IMGT <400> 35 Ile Asn Pro Lys Tyr Gly Thr Thr 1 5 <210> 36 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> HA, HB, HC, HD CDR3, IMGT <400> 36 Thr Arg Gly Leu Asn Ala Trp Asp Tyr 1 5 <210> 37 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> LA, LB, LC, LD, LG, LH CDR1, KABAT <400> 37 Gly Ala Ser Glu Asn Ile Tyr Gly Ala Leu Asn 1 5 10 <210> 38 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> LA, LB, LD, LE, LH CDR2, KABAT <400> 38 Gly Ala Thr Asn Leu Ala Asp 1 5 <210> 39 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> LA, LB, LC, LD, LE, LF, LG, LH CDR3, KABAT <400> 39 Gln Asn Val Leu Thr Thr Pro Tyr Thr 1 5 <210> 40 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> LE, LF CDR1, KABAT <400> 40 Gln Ala Ser Glu Asn Ile Tyr Gly Ala Leu Asn 1 5 10 <210> 41 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> LC, LF CDR2, KABAT <400> 41 Gly Ala Thr Asn Leu Ala Thr 1 5 <210> 42 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> LG CDR2, KABAT <400> 42 Gly Ala Thr Asn Leu Glu Asp 1 5 <210> 43 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> LA, LB, LC, LD, LE, LF, LG, LH CDR1, IMGT <400> 43 Glu Asn Ile Tyr Gly Ala 1 5 <210> 44 <211> 3 <212> PRT <213> Artificial Sequence <220> <223> LA, LB, LC, LD, LE, LF, LG, LH CDR2, IMGT <400> 44 Gly Ala Thr 1 <210> 45 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> LA, LB, LC, LD, LE, LF, LG, LH CDR3, IMGT <400> 45 Gln Asn Val Leu Thr Thr Pro Tyr Thr 1 5

Claims

1. An isolated anti-αvβ6 antibody or antigen-binding fragment thereof comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region is (i) CDR-H1 comprising the amino acid sequence of SEQ ID NO: 31; (ii) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 32, and (iii) CDR-H3 comprising the amino acid sequence of SEQ ID NO: 33 Includes; The light chain variable region is (i) CDR-L1 comprising the amino acid sequence of SEQ ID NO: 37; (ii) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 42, and (iii) CDR-L3 comprising the amino acid sequence of SEQ ID NO: 39 and wherein the CDRs are determined by Kabat, or an antigen-binding fragment thereof.

2. An isolated anti-αvβ6 antibody or antigen-binding fragment thereof comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region is (i) CDR-H1 comprising the amino acid sequence of SEQ ID NO: 34; (ii) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 35, and (iii) CDR-H3 comprising the amino acid sequence of SEQ ID NO: 36 Including, The light chain variable region is (i) CDR-L1 comprising the amino acid sequence of SEQ ID NO: 43; (ii) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 44, and (iii) CDR-L3 comprising the amino acid sequence of SEQ ID NO: 45 and wherein the CDRs are determined by IMGT.

3. 3. The antibody or antigen-binding fragment of claim 1 or claim 2, wherein the antibody is humanized.

4. 4. The antibody or antigen-binding fragment of claim 1, wherein the heavy chain variable region comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 6 and the light chain variable region comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:

17.

5. 5. The antibody or antigen-binding fragment of claim 1, wherein the heavy chain variable region comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 6 and the light chain variable region comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:

17.

6. 6. The antibody or antigen-binding fragment of claim 1 , wherein the heavy chain variable region comprises an amino acid sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 6 and the light chain variable region comprises an amino acid sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO:

17.

7. 7. The antibody or antigen-binding fragment of claim 1, wherein the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 6 and the light chain variable region comprises the amino acid sequence of SEQ ID NO:

17.

8. 8. The antibody or antigen-binding fragment of claim 4, wherein H2 is occupied by F, H28 is occupied by S, H48 is occupied by I, H67 is occupied by A, H69 is occupied by L, H71 is occupied by V, H73 is occupied by K, H78 is occupied by A, H93 is occupied by T, L69 is occupied by R, and L71 is occupied by Y, and the numbering is according to the Kabat numbering system.

9. 9. The antibody or antigen-binding fragment of claim 1 , wherein the heavy chain has an amino acid sequence comprising SEQ ID NO: 21 and the light chain has an amino acid sequence comprising SEQ ID NO:

29.

10. The antibody or antigen-binding fragment is an antigen-binding fragment, and the antigen-binding fragment is Fab, Fab', F(ab') 2 10. The antibody or antigen-binding fragment of any one of claims 1 to 9, selected from the group consisting of: Fab'-SH, Fv, diabody, linear antibody, and single-chain antibody fragment.

11. 11. The antibody or antigen-binding fragment of claim 1, wherein the heavy chain variable region of the antibody is fused to a heavy chain constant region and the light chain variable region is fused to a light chain constant region.

12. 12. The antibody or antigen-binding fragment of claim 11, wherein the heavy chain constant region is of the IgG1 isotype.

13. 13. An antibody drug conjugate comprising the antibody or antigen-binding fragment of any one of claims 1 to 12 conjugated to a cytotoxic or cytostatic agent.

14. 14. The antibody-drug conjugate of claim 13, wherein the antibody or antigen-binding fragment is conjugated to a cytotoxic or cytostatic agent via a linker.

15. 15. The antibody drug conjugate of claim 13 or 14, wherein the cytotoxic or cytostatic agent is monomethyl auristatin.

16. 16. The antibody drug conjugate of any one of claims 13 to 15, wherein the monomethylauristatin is monomethylauristatin E (MMAE).

17. 17. The antibody drug conjugate of claim 16, wherein the antibody or antigen-binding fragment thereof is conjugated to MMAE via an enzyme-cleavable linker unit.

18. 18. The antibody drug conjugate of claim 17, wherein the enzyme-cleavable linker unit comprises a Val-Cit linker.

19. The antibody or antigen-binding fragment thereof has the formula: a -W w -Y y -A- is a stretcher unit, a is 0 or 1, -W- is an amino acid unit, w is an integer ranging from 0 to 12, and -Y- is a spacer unit, and y is 0, 1, or 2.

20. 20. The antibody drug conjugate of claim 19, wherein the Stretcher unit has the structure of formula I below, and the Amino Acid unit is Val-Cit; and the Spacer unit is a p-aminobenzyl alcohol (PAB) group having the structure of formula II below. 【Chemistry 1】

21. The linker is attached to monomethyl auristatin E to form the following structure: 【Chemistry 2】 (wherein Ab is the antibody h2A2 and p is a number from 1 to 16). The antibody drug conjugate of any one of claims 14 to 20, wherein the antibody drug conjugate has the formula:

22. 22. The antibody drug conjugate of claim 21, wherein the average value of p in a population of antibody drug conjugates is about 4.

23. 13. A nucleic acid encoding a heavy chain variable region and / or a light chain variable region defined by any one of claims 1 to 12.

24. A vector comprising the nucleic acid of claim 23.

25. 25. The vector of claim 24 which is an expression vector.

26. 26. A host cell comprising the nucleic acid of claim 25.

27. 27. The host cell of claim 26, which is a Chinese Hamster Ovary (CHO) cell.

28. A method for producing an anti-αvβ6 antibody or an antigen-binding fragment thereof, comprising culturing a host cell described in claim 26 or 27 under conditions suitable for the production of an anti-αvβ6 antibody or an antigen-binding fragment thereof.

29. The method of claim 28, further comprising isolating the anti-αvβ6 antibody or antigen-binding fragment thereof produced by the host cell.

30. A method for producing an anti-αvβ6 antibody-drug conjugate, comprising culturing a host cell described in claim 26 or 27 under conditions suitable for the production of an anti-αvβ6 antibody, isolating the anti-αvβ6 antibody produced from the host cell, and conjugating the anti-αvβ6 antibody to a cytotoxic agent or a cell proliferation inhibitor.

31. The method of claim 30, wherein the anti-αvβ6 antibody is conjugated to a cytotoxic or cytostatic agent via a linker.

32. The method of claim 31 , wherein the linker is a Val-Cit linker.

33. 33. The method of any one of claims 30 to 32, wherein the cytotoxic or cytostatic agent is monomethyl auristatin E (MMAE).

34. 23. A method of treating cancer in a subject, comprising administering to the subject an antibody or antigen-binding fragment of any one of claims 1 to 12, or an antibody drug conjugate of any one of claims 13 to 22.

35. 35. The method of claim 34, wherein the cancer is selected from the group consisting of non-small cell lung cancer (NSCLC), head and neck cancer, esophageal cancer, breast cancer, ovarian cancer, bladder cancer, skin cancer (SCC), ovarian cancer, cervical cancer, gastric cancer, and pancreatic cancer.

36. 35. The method of claim 34, wherein the lung cancer is non-small cell lung cancer.

37. 35. The method of claim 34, wherein the cancer is head and neck cancer.

38. 38. The method of any one of claims 34 to 37, wherein the antibody or antigen-binding fragment or antibody drug conjugate is in a pharmaceutical composition comprising the antibody or antigen-binding fragment or antibody drug conjugate and a pharma- ceutically acceptable carrier.

39. 39. The method of any one of claims 34 to 38, wherein the subject is a human.

40. A pharmaceutical composition comprising an antibody or antigen-binding fragment of any one of claims 1 to 12, or an antibody-drug conjugate of any one of claims 13 to 22, and one or more agents selected from the group consisting of physiologically acceptable carriers, diluents, excipients and adjuvants.

41. 41. The pharmaceutical composition of claim 40, administered in combination with radiation or a chemotherapeutic agent.

42. 1. An antibody drug conjugate comprising an isolated anti-αvβ6 antibody conjugated to vcMMAE, wherein the antibody has a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:6 and a light chain variable region comprising the amino acid sequence of SEQ ID NO:17, and the antibody drug conjugate has the following structure: 【Chemistry 3】 (wherein Ab is an antibody and p is a number from 1 to 16).

1. An antibody-drug conjugate comprising:

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