Composition

Abstract

To provide compositions having an effect of suppressing fat accumulation in adipocytes and an effect of activating myoblasts, and having excellent effects such as reducing body fat and strengthening muscles.SOLUTION: A composition of the present invention contains: two or more ingredients selected from processed plant products and sugar alcohols; and a malto-oligosaccharide. Preferably, it contains a processed plant product and a sugar alcohol and a digestible oligosaccharide, or contains a processed product of two or more different species of plants, and a digestible oligosaccharide. Preferably, the digestible oligosaccharide is a malto-oligosaccharide having a trisaccharide or more and a hexasaccharide or less. Preferably, the sugar alcohol is a sugar alcohol having a tetrasaccharide or less.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a composition containing two or more components selected from a processed plant product and a sugar alcohol, and a digestible oligosaccharide. [Background technology]

[0002] Conventionally, compositions containing processed plant products have been known as health foods and the like (Patent Document 1). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] JP 2003-267880 A Summary of the Invention [Problem to be solved by the invention]

[0004] With regard to compositions used in health foods and the like, consumer needs have become more diverse in recent years, and the demand for further improved effects, particularly in terms of health and beauty, is becoming stronger. However, conventional compositions have not been able to fully meet this demand. [Means for solving the problem]

[0005] Therefore, the present inventors have intensively studied a composition containing at least one selected from a processed plant product and a sugar alcohol, which can further enhance the effects from the viewpoints of health and beauty. As a result, they have surprisingly found that a combination of digestible oligosaccharides with two or more selected from a processed plant product and a sugar alcohol has an excellent effect of inhibiting fat accumulation in adipocytes and activating myoblasts, and is useful for dieting and preventing or reducing locomotive syndrome, and have thus completed the present invention.

[0006] The present invention is based on the above findings and provides a composition containing two or more members selected from a processed plant product and a sugar alcohol, and a digestible oligosaccharide. Effect of the Invention

[0007] According to the present invention, a composition having excellent effects of inhibiting fat accumulation in adipocytes and activating myoblasts is provided. Also, according to the present invention, a diet composition and / or a composition for preventing locomotive syndrome can be provided, which can inhibit the accumulation of body fat such as subcutaneous fat by inhibiting fat accumulation in adipocytes and activating myoblasts, maintain or improve energy metabolic activity such as lipid decomposition and burning, reduce or inhibit weight gain, slim down the appearance, inhibit rebound, build muscle or prevent muscle loss, and improve the efficiency of recovery from muscle damage. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0008] The composition of the present invention will be described below based on its preferred embodiment. Hereinafter, the composition of the present invention can be any of "oral composition", "oral agent", "edible composition", "edible agent", and "food and drink", and can also be any of "diet composition", "muscle building composition", "muscle reduction prevention composition", "composition for preventing and / or improving locomo syndrome", "muscle building agent", "muscle reduction prevention agent", "agent for preventing and / or improving locomo syndrome", "diet food", "muscle building food", "muscle reduction prevention food", and "food for preventing and / or improving locomo syndrome" that can safely obtain the effects of the present invention.

[0009] Digestive oligosaccharides The digestive oligosaccharide in the present invention is an oligosaccharide of three or more sugars that is decomposed by human digestive enzymes and absorbed in the stomach or small intestine to become energy. The number of constituent sugars of the digestive oligosaccharide that can be used in the present invention is preferably three to ten saccharides, more preferably three to octasaccharides, even more preferably three to hexasaccharides, and particularly preferably three to tetrasaccharides from the viewpoint of effectiveness. Examples of digestive oligosaccharides include maltooligosaccharide, isomaltooligosaccharide, soybean oligosaccharide, etc., and maltooligosaccharide is particularly preferred from the viewpoint of availability, enhancing the effect of suppressing fat accumulation in adipocytes and activating myoblasts, and good handling during production. Specific examples of maltooligosaccharides include maltotriose, maltotetraose, maltopentaose, maltohexaose, etc., and maltotriose is particularly preferred from the viewpoint of enhancing the effect.

[0010] The content of digestive oligosaccharides in the solid content of the composition of the present invention is preferably 0.0001% by mass or more, more preferably 0.001% by mass or more, even more preferably 0.01% by mass or more, and particularly preferably 0.05% by mass or more in terms of effect, in terms of further enhancing the effect of inhibiting fat accumulation in adipocytes and the activation of myoblasts. The composition of the present invention may be composed of only digestive oligosaccharides, processed plant products, and sugar alcohols, but from the viewpoint of enabling high functionality and multi-functionality by combination with other components, the upper limit of the content of digestive oligosaccharides in the composition is preferably 30% by mass or less, more preferably 20% by mass or less, and even more preferably 10% by mass or less.

[0011] ·Plant processed products or sugar alcohols The composition of the present invention contains a combination of two or more selected from processed plant products and sugar alcohols. The combination of two or more selected from processed plant products and sugar alcohols is preferably a combination of two or more processed plant products, or a combination of one or more processed plant products and one or more sugar alcohols. The combination of two or more processed plant products is preferably a combination of processed products of two or more different plants.

[0012] The processed plant product in the present invention is obtained by subjecting a plant to one or more of the following processes: drying, crushing, extraction, filtration, squeezing, slurrying, fermentation, heating, etc. Examples of plant parts include leaves, roots, rhizomes, flowers, stems, seeds, etc. The leaves may include stems.

[0013] Specific examples of plant processed products include, but are not limited to, dried powder obtained by drying and pulverizing a plant body (hereinafter also referred to as "dried pulverized powder"); shredded plant body and its dried product; squeezed juice of a plant body and its dried powder; and extract of a plant body and its dried powder. In the present invention, it is preferable that the final product is in the form of a powder from the viewpoint of ease of processing, storage, transportation, etc. and versatility of use. In this specification, the term "powder" generally includes any of dried pulverized powder, dried powder of shredded product, dried powder of squeezed juice, dried powder of extract, and dried powder of fermented product. Powder naturally includes granular and granular forms.

[0014] A conventionally known method can be used to dry and pulverize the plant body into powder. As such a method, a method in which a drying treatment and a pulverization treatment are combined can be used for the plant body. Either the drying treatment or the pulverization treatment may be performed first, but it is preferable to perform the drying treatment first. The drying and pulverization treatment may further include one or more treatments selected from blanching treatment, cooling treatment, sterilization treatment, etc., as necessary. The pulverization treatment may be performed two or more times, and it is preferable to perform a coarse pulverization treatment followed by a fine pulverization treatment for more fine pulverization.

[0015] The drying treatment is not particularly limited, but is preferably a treatment for drying barley stems and leaves so that the moisture content is 10% by mass or less, particularly 5% by mass or less. This drying treatment can be carried out by any method known to those skilled in the art, such as hot air drying, high-pressure steam drying, electromagnetic wave drying, freeze drying, etc.

[0016] The crushing treatment is not particularly limited, and examples thereof include a treatment in which the plant body is crushed by any method commonly used by those skilled in the art using crushing equipment or tools such as a crusher, a mill, a blender, or a stone mill. The crushed plant body is sieved as necessary, and it is preferable to use, for example, a sieve that passes through a 30 to 250 mesh as the plant body powder. By making the particle size smaller than 250 mesh, the plant body powder becomes easier to handle during further processing, and by making the particle size larger than 30 mesh, it becomes easier to uniformly mix the plant body powder with other materials.

[0017] Specific examples of the drying and pulverization method include a method in which the plant body is cut, dried to a water content of 10% by mass or less, and then pulverized. Other examples include a method in which the plant body is cut, rolled, dried, and pulverized; a method in which the plant body is dried, coarsely pulverized, heated at 110° C. or higher, and then finely pulverized.

[0018] The method for shredding the plant body is not particularly limited, and any method that is commonly used by those skilled in the art when shredding a plant body, such as slicing, crushing, or chopping, may be used. As an example of shredding, the plant body may be made into a slurry. The slurry is made by putting the plant body into a mixer, juicer, blender, mass colloider, or the like to make it into a mushy porridge-like substance (a suspension of liquid and solid). When shredding the seeds is heated, the liquid may be boiled down with water, and the coarse solid content may be removed by sieving, filtration, or the like, and the liquid may be used.

[0019] The method for squeezing the plant body is not particularly limited, and examples thereof include a method of squeezing the plant body or a shredded product thereof, a method of centrifuging or filtering a shredded product of the plant body, etc. Specific examples of the squeezing method include a method in which the juice is squeezed by a mechanical crushing means such as a mixer or a juicer, and if necessary, a crude solid content is removed by a means such as sieving or filtration to obtain a squeezed juice.

[0020] The method for obtaining an extract of a plant body is not particularly limited, but examples thereof include a method in which an extraction solvent commonly used by those skilled in the art, such as ethanol, water, aqueous ethanol, methanol, or ethyl acetate, is added to the plant body or its finely cut or dried product, and the extraction is performed by stirring and / or heating as necessary. One or more extraction solvents can be appropriately selected and used as necessary. When heating, the temperature is not particularly limited as long as it is room temperature to the boiling point of the solvent. It is preferable to use an extract from which crude solids have been removed by subsequent sieving, filtration, or other means. For example, the filtrate (extract) obtained by adding water to finely cut soybeans (ground product), boiling it down, and then filtering it is known as soy milk. The extract may be concentrated as necessary, or dried and powdered. When powdering, an excipient may be used as necessary.

[0021] When the processed plant product is in a powder form, the moisture content is preferably 20% by mass or less, particularly 10% by mass or less, from the viewpoints of stability and prevention of quality deterioration, etc. For example, a moisture content of 1% by mass or more is preferred from the viewpoint of ease of production of the powdered processed plant product.

[0022] When the plant processed product is in a powder form, it is preferable that the powder passes through any one of 30 to 250 mesh sieves in terms of ease of mixing with other ingredients, ease of oral administration, etc. From the same viewpoint, it is more preferable that 90% by mass or more of the powdered plant processed product passes through a 200 mesh sieve.

[0023] The plant body may also be a fermented product, for example, obtained by adding lactic acid bacteria, yeast, koji mold, natto bacteria, acetic acid bacteria, etc. to the plant body or its crushed product, juice, extract, or shredded product and fermenting it.

[0024] The juice obtained by the above-mentioned shredding process, the liquid extract obtained by the extraction process, and the liquid or slurry after fermentation can all be dried and powdered by any method known to those skilled in the art, such as hot air drying, high-pressure steam drying, electromagnetic wave drying, freeze-drying, etc. In this case, an excipient such as dextrin may be added.

[0025] The content of two or more selected from the processed plant products and sugar alcohols in the present invention is not particularly limited, but from the viewpoint of further enhancing the effect of inhibiting fat accumulation in adipocytes and the effect of activating myoblasts, the total content of two or more selected from the processed plant products and sugar alcohols in the solid content of the composition of the present invention is preferably 10% by mass or more, more preferably 20% by mass or more, even more preferably 30% by mass or more, and particularly preferably 40% by mass or more in terms of effect, in terms of dry mass. The composition of the present invention may be composed of only two or more selected from digestible oligosaccharides, processed plant products, and sugar alcohols, but from the viewpoint of enabling high functionality and multi-functionality by combination with other components, the upper limit of the content of two or more selected from the processed plant products and sugar alcohols in the composition is preferably 90% by mass or less, more preferably 80% by mass or less, and even more preferably 70% by mass or less. The total amount of two or more types selected from plant processed products and sugar alcohols in the composition referred to here means the total amount when only two or more types selected from plant processed products and sugar alcohols are contained, and means the total amount when three or more types are contained.

[0026] When the composition of the present invention contains a processed plant product, in order to further enhance the effects of inhibiting fat accumulation in adipocytes and activating myoblasts, the content of the processed plant product in the solid content of the composition of the present invention is preferably 0.01 parts by mass or more, more preferably 0.1 parts by mass or more, even more preferably 1 part by mass or more, even more preferably 10 parts by mass or more, even more preferably 50 parts by mass or more, particularly preferably 100 parts by mass or more, and particularly preferably 200 parts by mass or more, in terms of dry mass relative to 1 part by mass of digestive oligosaccharides. In addition, from the viewpoint of enabling high functionality and multi-functionality by combination with other components, the upper limit of the content of the processed plant product in the composition is preferably 10,000 parts by mass or less, more preferably 1,000 parts by mass or less, and even more preferably 500 parts by mass or less, relative to 1 part by mass of digestive oligosaccharides. The amount of the processed plant product in the composition is the amount of the processed plant product alone when only one type of processed plant product is contained, and is the total amount when two or more types of processed plant products are contained.

[0027] The processed plant product of the present invention may be a commercially available product, for example, the one described in the Examples below.

[0028] The plant body in the processed plant product is preferably a processed product of green leaves. In the present invention, green leaves are leaves of green plants that can be orally ingested and have chlorophyll. As green leaves that can be used in the present invention, for example, green leaves of Gramineae plants, Asteraceae plants, Umbelliferae plants, Mulberry plants, Houttuynia plants, Labiatae plants, Liliaceae plants, Tilia plants, Convolvulaceae plants, Theaceae plants, etc. are preferable because they have high fat accumulation inhibitory activity in adipocytes and myoblast activation activity, and because plant-derived nutritional components can be ingested. Examples of Gramineae plants include wheat, durum wheat, rye, triticale, barley, oats, pigeon oats, corn, rice, barnyard millet, foxtail millet, and kumazasa. Examples of Asteraceae plants include mugwort. Examples of Umbelliferae plants include Angelica keiskei, parsley, celery, and longevity grass (also called Peony grass). Examples of plants belonging to the family Moraceae include mulberry and the like. Examples of plants belonging to the family Houttuynia include Houttuynia cordata and the like. Examples of plants belonging to the family Lamiaceae include perilla and the like. Examples of plants belonging to the family Brassicaceae include komatsuna, kale, cabbage, broccoli and the like. Examples of plants belonging to the family Liliaceae include asparagus and the like. Examples of plants belonging to the family Tiliaceae include mulukhiyah and the like. Examples of plants belonging to the family Convolvulaceae include sweet potato and the like. Examples of plants belonging to the family Theaceae include tea and the like. Among these, barley, kale, chou-mei-sou, kumazasa, tea, angelica tree, mulberry, sweet potato, mugwort, and cabbage are particularly preferred in terms of effectiveness.

[0029] When the composition of the present invention contains a combination of processed products of two or more different plants, it is preferable to use a combination of processed green leaf products in order to enhance fat accumulation suppression activity in adipocytes and myoblast activation activity. In addition, as a combination of two or more processed plant products, a combination of a processed product of wheat or barley and a processed product of a plant other than wheat or barley is particularly preferable in terms of effectiveness. From these points of view, a combination of a processed product of wheat or barley green leaves and a processed product of a plant other than wheat or barley is preferable. Examples of plants other than wheat or barley include grasses (excluding wheat or barley), Asteraceae plants, Apiaceae plants, Mulberry plants, Houttuynia plants, Lamiaceae plants, Liliaceae plants, Tilia plants, Convolvulaceae plants, and Theaceae plants.

[0030] When barley is used as the plant, it is preferable to use young barley leaves. Young barley leaves are leaves and / or stems harvested before maturity, i.e., from the start of tillering to the start of ear emergence. When sweet potatoes are used, it is preferable to use leaves or leaves including stems that are exposed above ground during cultivation of sweet potatoes, and young sweet potato leaves are preferable.

[0031] When the plant body is green leaves, the green leaves are preferably in the form of dried and pulverized powder. As described above, the dried and pulverized powder is a dry powder obtained by drying and pulverizing a plant body that has not been subjected to juice squeezing or extraction processing.

[0032] In particular, when the composition of the present invention contains processed wheat products, in order to further enhance the effects of inhibiting fat accumulation in adipocytes and activating myoblasts, the content thereof is preferably from 1 to 5,000 parts by mass, in dry mass, per 1 part by mass of digestible oligosaccharides, more preferably from 100 to 2,500 parts by mass, and even more preferably from 1,000 to 1,250 parts by mass.

[0033] Furthermore, when the composition of the present invention is used in combination with a processed wheat or barley product and a processed plant other than wheat or barley, in terms of myoblast activation activity and fat accumulation inhibitory activity in adipocytes, particularly in terms of fat accumulation inhibitory activity, the content of the processed plant other than wheat or barley product is preferably 0.01 to 1,000 parts by mass, in dry mass, per 1 part by mass of the processed wheat or barley product, more preferably 0.1 to 100 parts by mass, even more preferably 1 to 10 parts by mass, and particularly preferably 5 to 8 parts by mass in terms of effect.

[0034] In particular, from the standpoint of further enhancing the fat accumulation prevention effect and myoblast activation activity, the mass ratio of digestible oligosaccharides to processed products of young barley leaves, kale, chou-mei-sou, kumazasa, tea, angelica tree, mulberry leaves, young sweet potato leaves, and mugwort leaves is preferably 0.01 mass parts or more of each processed product on a dry mass basis to 1 mass part of digestible oligosaccharides, more preferably 0.1 mass parts or more and 10,000 mass parts or less, even more preferably 1 mass part or more and 1,000 mass parts or less, and particularly preferably 10 mass parts or more and 100 mass parts or less from the standpoint of effect.

[0035] Examples of sugar alcohols that can be used in the present invention include monosaccharide alcohols, disaccharide alcohols, and trisaccharide or higher alcohols. Examples of monosaccharide alcohols include pentitols such as erythritol and xylitol, and hexitols such as D-glucitol (sorbitol). Examples of disaccharide alcohols include reduced maltose (maltitol), lactitol, reduced palatinose (isomalt), and the like. Examples of trisaccharide or higher alcohols include maltotriitol, isomaltotriitol, and panitol. As sugar alcohols, from the viewpoint of easily obtaining the effect of activating myoblasts and inhibiting fat accumulation by combining with digestible oligosaccharides, it is preferable that at least one type selected from alcohols of tetrasaccharides or less is used, and more preferably at least one type selected from alcohols of trisaccharides or less is used, and particularly, it is preferable that at least one type selected from alcohols of disaccharides or less is used, and reduced maltose is preferable as the alcohol of disaccharides or less from the viewpoint of effect.

[0036] When the composition of the present invention contains sugar alcohol, in order to further enhance the above-mentioned myoblast activation effect and fat accumulation suppression effect in adipocytes, the content of sugar alcohol in the solid content of the composition of the present invention is preferably 1 part by mass or more, more preferably 10 parts by mass or more, even more preferably 100 parts by mass or more, even more preferably 1000 parts by mass or more, and particularly preferably 10000 parts by mass or more in terms of effect, in terms of dry mass, per 1 part by mass of digestive oligosaccharide. In addition, from the viewpoint of enabling high functionality and multi-functionality by combination with other components, the upper limit of the content of sugar alcohol in the composition is preferably 10000000 parts by mass or less, more preferably 1000000 parts by mass or less, even more preferably 100000 parts by mass or less, and particularly preferably 10000 parts by mass or less in terms of effect. The amount of sugar alcohol in the composition is the amount of the single sugar alcohol when only one type of sugar alcohol is contained, and is the total amount when two or more types of sugar alcohols are contained.

[0037] The composition of the present invention may contain other components in addition to digestible oligosaccharides, processed plant products, and sugar alcohols. Examples of the other components include vitamins, proteins, minerals, dairy products, microorganisms such as lactic acid bacteria, citric acid, acidulants, colorants, glossing agents, as well as manufacturing agents such as talc, cellulose, and calcium stearate. Other components include various excipients, binders, lubricants, stabilizers, diluents, bulking agents, emulsifiers, colorants, flavorings, food additives, and seasonings. The content of the other components can be appropriately selected depending on the form of the composition. In the present invention, the components other than digestible oligosaccharides, processed plant products, and sugar alcohols contained in the composition are preferably 90% by mass or less, more preferably 60% by mass or less, and particularly preferably 30% by mass or less in terms of effectiveness, based on the solid content.

[0038] The composition of the present invention may be in any form, such as solid, semi-solid, or fluid. For example, the solid form may be a tablet, capsule, bar, plate, block, solid, round, candy, tablet, gummy, wafer, biscuit, cookie, cake, chewable, or stick. The semi-solid form may be a paste or jelly. The fluid form may be a syrup, liquid, or jelly.

[0039] In addition, the composition of the present invention is preferably in the form of powder or granules, and is mixed with water and taken orally, since it prevents spoilage and is suitable for long-term storage.In addition, when the composition of the present invention is in the form of solid, as described above, it can be mixed with water to make a liquid, and the liquid can be taken orally, for example, by drinking, but it can also be taken orally as a solid, depending on the preferences of the person who takes it.In addition, it can be added to not only water, but also milk, soy milk, fruit juice drinks, whey drinks, soft drinks, yogurt, pancake mix, etc. for use.It can also be used as a supplement, health food, nutritional functional food, functional food, specified health food, and medicine.

[0040] The composition of the present invention is an oral composition, and when the composition contains a processed green leaf product as a processed plant product, it is preferable to use the composition of the present invention as an edible composition for green juice. The edible composition for green juice is a beverage containing a processed green leaf product. The edible composition for green juice includes the beverage and a solid that is dispersed or dissolved in a liquid to obtain the beverage. The edible composition for green juice includes smoothies, jellies, etc. in addition to commonly known green juice products.

[0041] The form of the composition of the present invention is not limited to the beverages given as examples of edible compositions for green juice and the solid form that is dispersed or dissolved in a liquid to obtain this beverage, and any form can be adopted.

[0042] As described in the examples below, the green leaf powder of the present invention contains two or more selected from processed plant products and sugar alcohols, and digestible oligosaccharides, and thus has excellent effects of inhibiting fat accumulation in adipocytes and activating myoblasts. Specifically, by ingesting a composition containing two or more selected from processed plant products and sugar alcohols, and digestible oligosaccharides, it is possible to prevent lipid accumulation in adipocytes and activate myoblasts in skeletal muscles, etc. Therefore, by ingesting the composition of the present invention, it is possible to prevent body fat accumulation, reduce body fat, maintain and promote energy metabolism in muscles, promote muscle building, prevent muscle attenuation and reduction, and prevent and improve locomotive syndrome. Therefore, the composition of the present invention can be excellent for preventing body fat accumulation, reducing body fat, activating myoblasts, promoting muscle cell proliferation, building muscle, preventing muscle loss, preventing muscle attenuation, maintaining and / or promoting metabolism, preventing obesity, eliminating obesity, reducing or suppressing weight gain, slimming one's appearance, preventing rebound, dieting, preventing and / or improving locomotive syndrome, etc. Here, building muscle means increasing muscle mass, and preventing muscle loss means preventing muscle mass loss. EXAMPLES

[0043] The present invention will be described in more detail below by showing examples, but the scope of the present invention is not limited to these examples.

[0044] [Examples 1 to 19, Comparative Examples 1 to 12] The following were used as test samples. Maltotriose: A commercially available powdered product was used. Young barley leaves: Dried and ground powder of young barley leaves manufactured by Toyo Shinyaku Co., Ltd. was used. The dried and ground powder had 90% or more by mass passing through a 200 mesh and a moisture content of 1% to 7% by mass. Kale: Dried and ground kale leaves manufactured by Toyo Shinyaku Co., Ltd. were used. More than 90% by mass of the dried and ground powder passed through a 200 mesh sieve, and the moisture content was between 1% and 7% by mass. · Chomeisou: Dried and crushed powder of Chomeisou leaves manufactured by Toyo Shinyaku Co., Ltd. The dried and crushed powder had 90% or more by mass passing through a 200 mesh and a moisture content of 1% to 7% by mass. · Kumazasa: Dried and crushed powder of Kumazasa leaves manufactured by Toyo Shinyaku Co., Ltd. More than 90% of the dried and crushed powder passed through a 200 mesh sieve, and the moisture content was between 1% and 7% by mass. Matcha: Dried and ground powder of tencha produced by Toyo Shinyaku Co., Ltd. was used. More than 90% of the dried and ground powder passed through a 200 mesh sieve, and the moisture content was between 1% and 7% by mass. · Angelica keiskei: Dried and crushed powder of Angelica keiskei leaves manufactured by Toyo Shinyaku Co., Ltd. The dried and crushed powder had 90% or more by mass passing through a 200 mesh and a moisture content of 1% to 7% by mass. Mulberry: Dried and crushed powder of mulberry leaves was used, manufactured by Toyo Shinyaku Co., Ltd. The dried and crushed powder had 90% or more by mass passing through a 200 mesh and a moisture content of 1% to 7% by mass. · Young sweet potato leaves: We used dried and ground powder of sweet potato leaves manufactured by Toyo Shinyaku Co., Ltd. Of the dried and ground powder, 90% by mass or more passed through a 200 mesh and the moisture content was 1% by mass or more and 7% by mass or less. Mugwort: Dried and crushed powder of mugwort leaves manufactured by Toyo Shinyaku Co., Ltd. was used. More than 90% of the dried and crushed powder passed through a 200 mesh sieve, and the moisture content was between 1% and 7% by mass. Reduced maltose: A commercially available powdered product was used. Trehalose: A commercially available powdered product was used. The above test samples were subjected to a fat accumulation test according to the following procedures (a) to (L). [Fat accumulation test] (a) 37℃, 5% CO by volume 2 In the incubator, 75 cm 2 Mouse fibroblast cells 3T3-L1 were cultured in a flask in DMEM medium containing 10% (v / v) FBS. (b) Fibroblast 3TL-L1 cells were suspended by trypsinization and placed at 75 cm 2 From the flask, transfer 2x10 cells to each well of a 96-well plate. 4The cells were seeded at a density of 100 cells / well and incubated at 37°C, 5% CO 2 The cells were pre-cultured in DMEM medium containing 10% (v / v) FBS in an incubator for 2 days, then the medium was replaced with differentiation-inducing medium containing the test sample (control was differentiation-inducing medium only), and the cells were cultured for 3 days to induce differentiation. The differentiation-inducing medium used was a 10% (v / v) FBS-containing DMEM medium containing 0.5 mM isobutylmethylxanthine, 0.5 μM dexamethasone, and 10 μg / mL insulin. The test samples were prepared in the differentiation-inducing medium so that the total amount of the test samples was a predetermined concentration (300 μg / mL concentration). Tables 1 and 2 show the mass ratio of each test sample to the total amount of the test samples. (c) The medium was replaced from the differentiation induction medium to a differentiation maintenance medium containing the test sample (control was differentiation maintenance medium only), and cultured for 4 days. As the differentiation maintenance medium, a 10% (v / v) FBS-containing DMEM medium containing 10 μg / mL insulin was used. The test samples were prepared in the differentiation maintenance medium so that the total amount was a specified concentration (300 μg / mL concentration). Tables 1 and 2 show the mass percentage of each test sample in the total amount of the test sample. (d) After culturing in (c), the culture medium supernatant was removed from the adipocytes. Then, 10% (v / v) formalin-containing PBS was added to the cells in an amount equal to that of the culture medium, and the cells were left to stand at room temperature for 10 minutes in the dark. The formalin solution was then removed from the cells, and the cells were washed once with PBS. (e) 10% (v / v) formalin-containing PBS was added to the cells at 100 μL / well, and the cells were then left to stand at room temperature for 10 minutes in the dark to fix the cells. (f) The formalin solution was removed, and the sections were washed twice with PBS. (g) 50 μL / well of 60% by volume isopropanol (staining solution) containing 3 mg / mL oil red was added to the adipocytes and blank wells, and the cells were stained by leaving the cells at room temperature for 30 minutes in the dark. The staining solution was then removed, and 150 μL / well of 60% by volume isopropanol aqueous solution was added to wash the cells twice. (h) 100% by volume isopropanol was added to the cells and blank wells at 100 μL / well, and the cells were shaken for about 10 minutes to extract the staining solution. The absorbance of the isopropanol solution from which the staining solution had been extracted was measured at 520 nm and 650 nm. (k) After completely removing the isopropanol solution by air drying, TM The protein amount of each cell was calculated using a BCA Protein Assay kit (Thermo Fisher Scientific). (L) The amount of fat accumulation per protein was calculated using the formula below, and the results of calculating the relative values ​​to the control are shown in Tables 1 and 2. A smaller relative value indicates that fat accumulation in fat cells is more suppressed. The results of the relative values ​​are shown in Tables 1 and 2 below. Relative fat accumulation (%) = [[(Abs520 sample - Abs520 blank) - (Abs650 sample - Abs650 blank)] / (Protein sample)] / [(Abs520 control - Abs520 blank) - (Abs650 control - Abs650 blank)] / (Protein control)]] x 100 (%) Abs520 sample, Abs650 sample: absorbance at 520 nm and 650 nm for each example or comparative example Abs520 control, Abs650 control: Absorbance of control at 520nm and 650nm Abs520 blank, Abs650 blank: Blank absorbance at 520nm and 650nm Protein sample: Amount of protein in cells in each Example or Comparative Example Protein control: The amount of protein in the cells in the control

[0045] [Table 1]

[0046] [Table 2]

[0047] [Table 3]

[0048] Table 1 shows the compositions and evaluation results of examples containing three components, a processed plant product, a sugar alcohol, and a digestible oligosaccharide, while Table 2 shows the compositions and evaluation results of examples containing digestible oligosaccharides and two or more types of processed plant products. Table 3 shows the compositions and evaluation results of comparative examples containing digestible oligosaccharides, a processed plant product alone, or trehalose and one type of processed plant product. It is generally known that differentiation of fibroblasts induces the expression of PPARγ, which activates fat synthesis and accumulates fat within the cells, resulting in large, round adipocytes. In each of the Examples shown in Tables 1 and 2, the relative absorbance value (turbidity) is low, even though the test sample concentration in the medium is low, compared to each of the Comparative Examples shown in Table 3. This shows that the compositions of each of the Examples shown in Tables 1 and 2 effectively suppress fat accumulation in fat cells, suppress body fat accumulation, and reduce body fat, compared to the compositions of Comparative Examples 1 to 11 containing each component shown in Table 3 alone. On the other hand, when the composition contains only trehalose, a disaccharide, and one type of processed plant product (Comparative Example 12), fat accumulation in fat cells is not effectively suppressed, indicating that excellent effects can be obtained by combining digestible oligosaccharides of trisaccharides or more with two or more specific components.

[0049] [Examples 20 to 32, Comparative Examples 13 to 22] Each of the above test sample powders was subjected to a myoblast activation test according to the following procedures (1) to (7). [Myoblast activation test] (1) Mouse skeletal muscle-derived myoblast cells (product name: C2C12, RIKEN BioResource Center) were incubated at 37°C and 5% CO 2Place a 75 cm 2 The cells were cultured in flasks. (2) After culturing in (1), cells were suspended by trypsinization and cultured at 75 cm 2 The cells were harvested from the flask and the number of cells was counted. Then, the cells were seeded in each well of a collagen-coated 96-well plate at a cell density of 2000 cells / well together with the medium, and incubated at 37°C, 5% CO 2 The cells were pre-cultured in an incubator for 24 hours. (3) Separately from (1) and (2), the powders shown in Tables 4 to 6 were dispersed or dissolved in 10 vol% FBS-containing DMEM medium to the concentrations shown in Tables 4 to 6 to prepare liquids, which were then filter-sterilized using a 0.2 μm filter (Advantec) to prepare sample liquids for the corresponding Examples or Comparative Examples. In Tables 4 to 6, "●" indicates that the sample liquid for the Example or Comparative Example contained the test sample in the left column at the concentration shown in the left column. As a control, 10 vol% FBS-containing DMEM medium itself was used as the sample liquid. (4) After removing the medium from each well, add 200 μL of the sample solution prepared in (3) to each well and incubate at 37°C and 5% CO 2 The cells were cultured in an incubator for 24 hours. (5) After the culture in (4), the medium was removed and each well was washed once with 200 μL / well of PBS. Then, 150 μL / well of Cell Counting Kit-8 solution (Dojindo Chemical Industries, Ltd.) diluted 30-fold with serum-free DMEM was added. (6) After adding the solution in (5), the plate was incubated at 37°C and 5% CO 2 After allowing the wells to stand in an incubator to allow for adequate color development, the absorbance of each well was measured at 450 nm. Based on the data obtained, the percentage of cells relative to the control (% of control) was calculated according to the following formula: % of control=(Data sample - Data blank) / (Data control - Data blank)×100 Data sample: absorbance of each example or comparative example Data control: Control absorbance Data blank: Absorbance when no cells are present

[0050] (7) Evaluation

[0051] The percentage of cell counts (% of control) for Examples 20 to 24, which combined digestible oligosaccharides, processed plant products, and sugar alcohols, was calculated by setting the percentage of cell counts for the corresponding comparative examples (see Table 6 below), which did not contain either the processed plant product or sugar alcohol in those Examples, at 100%. The values ​​shown in Table 4 are shown in Table 4.

[0052] [Table 4]

[0053] In addition, for the percentage of cell counts (% of control) in Examples 25 to 32, which combined digestible oligosaccharides with two types of plant processed products, the percentage of cell counts in the corresponding comparative examples (see Table 6 below), which did not contain either of the two types of plant processed products in those Examples, was calculated as 100%, and is shown in Table 5. [Table 5]

[0054] [Table 6]

[0055] As shown in Table 4, each example containing three components, namely, a processed plant product, sugar alcohol, and digestible oligosaccharides, shows significantly higher myoblast activation activity than the corresponding comparative example containing two components, namely, digestible oligosaccharides, and either a processed plant product or sugar alcohol. As shown in Table 5, each Example containing three components, digestive oligosaccharides and two kinds of processed plant products, has a significantly higher myoblast activation activity than the corresponding Comparative Example containing two components, digestive oligosaccharides and one kind of processed plant product. Comparative Example 17 could not be evaluated because it was below the detection limit. From the above, it was shown that by combining digestible oligosaccharides with two or more components selected from processed plant products and sugar alcohols, myoblasts are effectively activated and energy metabolism in muscle tissue is enhanced.

[0056] Example 33 (Production of a composition for green juice) Raw materials were prepared according to the mixing ratios described in the following formulation examples, and a composition for green juice exhibiting the effects of the present invention was manufactured.

[0057] [Table 7]

[0058] Example 34 (Production of a composition for green juice) Raw materials were prepared according to the mixing ratios described in the following formulation examples, and a composition for green juice exhibiting the effects of the present invention was manufactured.

[0059] [Table 8]

[0060] Example 35 (Production of a composition for green juice) Raw materials were prepared according to the mixing ratios described in the following formulation examples, and a composition for green juice exhibiting the effects of the present invention was manufactured.

[0061] [Table 9]

[0062] Example 36 (Production of a composition for green juice) Raw materials were prepared according to the mixing ratios described in the following formulation examples, and a composition for green juice exhibiting the effects of the present invention was manufactured.

[0063] [Table 10]

[0064] Example 37 (Preparation of tablets) The raw materials were prepared according to the mixing ratios described in the following formulation examples, and then tablets exhibiting the effects of the present invention were produced using a tablet press.

[0065] [Table 11]

[0066] Example 38 (Preparation of tablets) The raw materials were prepared according to the mixing ratios described in the following formulation examples, and then tablets exhibiting the effects of the present invention were produced using a tablet press.

[0067] [Table 12]

Claims

[Claim 1] The invention described in the specification.

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