Concomitant radiation therapy

Abstract

The present invention relates to methods of treating diseases and conditions characterized by abnormal cell proliferation (eg, cancer) comprising administering a combination of a DNA repair inhibitor and a molecularly targeted radioimmunotherapeutic agent.

Description

[Technical field]

[0001] The present invention relates to a combination therapy for the treatment of cancer comprising the administration of an inhibitor of DNA protein kinase (DNA-PK) and molecular targeted radiation therapy.

[0002] Related Applications This application claims priority to Australian Provisional Patent Applications Nos. 2021902557 and 2021902582, the contents of which are incorporated herein by reference in their entireties. [Background technology]

[0003] To ensure accurate maintenance and transmission of genetic information to offspring, mammalian cells have evolved sophisticated mechanisms to sense DNA damage, coordinate its repair, and prevent its potentially tumorigenic effects, collectively known as the DNA damage response (DDR). Defects in the DDR contribute to genomic instability and are one of the key hallmarks of cancer.

[0004] DNA can be damaged by multiple intrinsic and extrinsic factors.Many established therapies, such as radiotherapy and chemotherapy, that attack cancer cell DNA, are used in clinical practice, but provide limited benefits to patients with cancer.This is at least partly due to the ability of tumor cells to deal with DNA damage.

[0005] Diverse types of damage occur in DNA, ranging from base modifications to strand breaks, which can result in large deletions or genomic rearrangements. Of these, double-strand breaks (DSBs) are considered the most harmful and, if left unrepaired, can have fatal consequences for cells and organisms. DSB repair is accomplished by two major pathways: homology-directed repair (HR) and non-homologous end joining (NHEJ). HR requires an intact DNA strand as a template for break repair and is restricted to the S and G2 phases of the cell cycle. Therefore, HR is considered less error-prone than NHEJ. Conversely, NHEJ repairs DSBs in the absence of a template, resulting in repaired DNA changes. Nevertheless, NHEJ is functional in all phases of the cell cycle and is believed to be responsible for the repair of more than 80% of DSBs induced by ionizing radiation (IR) in cancer cells.

[0006] DNA-dependent protein kinase (DNA-PK) is a serine / threonine kinase and a key facilitator of NHEJ repair that works in concert with five other factors: Ku70, Ku80, XRCC4, ligase IV, and Artemis. A heterodimer consisting of Ku70 and Ku80 specifically binds to DSBs, recruits and activates the catalytic subunit DNA-PKc, which then recruits the XRCC4 / ligase IV heterodimer involved in resealing the break. Trimming of DSB ends may require Artemis and other DNA polymerases specialized for repair-mediated DNA polymerization. Activation of DNA-PK by autophosphorylation is essential for the proper execution of the repair process.

[0007] Enhancement of radiotherapy and chemotherapy by inhibiting DNA damage repair has been proposed as a therapeutic strategy to improve outcomes for patients with, for example, solid tumors, however, the success of such combination therapies has often been hampered by toxicity issues.

[0008] There remains a need for improved treatments for cancer and other disorders characterized by abnormal cellular function and proliferation, particularly for increasing the efficacy of such treatments while maintaining favorable toxicity and side effect profiles.

[0009] The reference to any prior art in this specification is not an admission or suggestion that this prior art forms part of the common general knowledge in any jurisdiction, or that this prior art would be understood, considered relevant, and / or could reasonably be expected to be combined with other prior art by a person skilled in the art. Summary of the Invention

[0010] In a first aspect, the present invention provides a method for treating a disease or disorder characterized by abnormal cell proliferation and function in a subject, comprising: i) DNA-PK inhibitors (DNA-PKi), ii) Molecularly targeted radiotherapeutic agents capable of being internalized in cells and / or retained in the blood circulation of a subject. administering to a subject in need of treatment a combination therapy comprising the radiotherapeutic agent comprises a radionuclide that is a beta emitter; Thereby, methods are provided for treating said disease or disorder characterized by abnormal cell proliferation and function in said subject.

[0011] In a second aspect, the present invention provides a method for treating a disease or disorder characterized by abnormal cell proliferation and function in a subject, comprising: i) (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharma- ceutically acceptable salt thereof; ii) Molecularly targeted radiotherapeutic agents capable of being internalized in cells and / or retained in the blood circulation of a subject. administering to a subject in need of treatment a combination therapy comprising thereby treating said disease or disorder characterized by abnormal cellular function in said subject.

[0012] In a preferred embodiment of any aspect of the invention, the molecularly targeted radiotherapeutic agent is a radioimmunoconjugate. Preferably, the radioimmunoconjugate comprises an antibody, or an antigen-binding fragment thereof, for binding to an antigen associated with the disease or disorder in need of treatment, conjugated to a radionuclide.

[0013] The antibody may be an antibody (e.g., a monoclonal antibody) that is itself an immunotherapeutic agent that binds to specific cells or proteins and then stimulates the patient's immune system to attack those cells. In this case, the radiotherapeutic agent acts in parallel with the immunotherapeutic effect of the antibody. Alternatively, the antibody may act only as a targeting agent and does not itself cause any immunotherapeutic effect. In this case, only the radionuclide complexed to the antibody acts as the active cell-destroying agent supported in the combination therapy method of the present invention by the DNA-PKi described herein.

[0014] In particularly preferred embodiments of any aspect of the invention, the molecularly targeted radiotherapeutic agent is a monoclonal antibody comprising an antigen binding domain for binding to an antigen associated with a disease or disorder in need of treatment, wherein said monoclonal antibody is conjugated to a radionuclide for providing a radiotherapeutic dose to cells expressing the antigen.

[0015] In any aspect of the invention, the disease or disorder characterized by abnormal cell proliferation or function is cancer, although it will be understood that the invention also applies to the treatment of other diseases or conditions in which cell replication is uninhibited, as described in more detail herein.

[0016] It will be appreciated that where the disease or disorder being treated is cancer, the molecularly targeted radiotherapeutic agent, preferably a radiolabeled antibody, will bind to a tumor-specific or tumor-associated antigen expressed by the cancer cells being treated.

[0017] In a preferred embodiment of any aspect of the invention, the disease or disorder to be treated is a cancer characterized by expression of carbonic anhydrase IX (CAIX). Thus, in such an embodiment, the molecular targeted radiotherapeutic agent preferably comprises an antibody or antigen-binding fragment thereof capable of specifically binding to CAIX. Examples of cancers that may be treated thereby include renal cell carcinoma (including clear cell renal cell carcinoma), colon cancer, breast cancer, lung cancer, cervical cancer, and melanoma.

[0018] In a preferred embodiment of any aspect of the present invention, the disease or disorder to be treated is a cancer characterized by the expression of prostate-specific membrane antigen (PSMA).Accordingly, in such an embodiment, the molecular targeted radiotherapy agent preferably comprises an antibody or an antigen-binding fragment thereof that can specifically bind to PSMA.Examples of cancers that can be treated thereby include prostate cancer, bladder cancer, testicular embryonal carcinoma, neuroendocrine cancer, renal cell carcinoma, and breast cancer.

[0019] According to any aspect of the invention, the radioimmunotherapeutic agent, preferably a radiolabeled monoclonal antibody, can be labeled with any suitable radionuclide that can be used to deliver a therapeutic dose of radiation to cells. Examples of suitable therapeutic radionuclides include astatine- 211 ( 211 At), Bismuth- 212 ( 212 Bi), Bismuth- 213 ( 213 Bi), Actinium- 225 ( 225 Ac), radium- 223 ( 223 Ra), lead- 212 ( 212 Pb), Thorium- 227 ( 227 Th), and terbium- 149 ( 149 In some embodiments, the radionuclide is an alpha emitter selected from the group consisting of: 225 In other embodiments, the radionuclide is 211 It is astatine.

[0020] In a preferred embodiment of the invention, the radionuclide is lutetium- 177 ( 177 Lu), yttrium- 90 ( 90 Y), iodine- 131 ( 131 l), Samarium- 153 ( 153 Sm), Holmium- 166 ( 166 Ho), Rhenium- 186 ( 186 Re), or rhenium- 188 ( 188 In some embodiments, the radionuclide is a beta emitter or a beta / gamma emitter selected from the group consisting of: 177 In another embodiment, the radionuclide is 188 It is rhenium.

[0021] In a particularly preferred embodiment of any aspect of the invention, the disease or disorder in need of treatment is a cancer characterized by expression of CAIX. Accordingly, there is provided a method for treating a cancer characterized by expression of CAIX, comprising: i) (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharma- ceutically acceptable salt thereof; ii) an antibody or antigen-binding fragment thereof for binding to CAIX, wherein said antibody or antigen-binding fragment thereof is conjugated to a radionuclide for delivering a radiotherapeutic dose to said cancer; administering to a subject in need of treatment a combination therapy comprising Preferably, the radionuclide is a beta emitter or a beta / gamma emitter and the antibody or antigen-binding fragment for binding to CAIX is one described herein, preferably comprising an antigen-binding domain comprising an amino acid sequence as defined in any of SEQ ID NO:52, SEQ ID NO:68, SEQ ID NO:84, SEQ ID NO:100, and SEQ ID NO:116, and an amino acid sequence as defined in any of SEQ ID NO:132, SEQ ID NO:148, SEQ ID NO:164, SEQ ID NO:180, SEQ ID NO:196, and SEQ ID NO:212, and most preferably, the antibody comprises the amino acid sequence as set forth in SEQ ID NO:231 and SEQ ID NO:234 ​​described herein.

[0022] In a particularly preferred embodiment, the disease or disorder in need of treatment is a cancer characterized by expression of PSMA. Thus, the present invention also provides a method for treating a cancer characterized by expression of PSMA, comprising: i) (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharma- ceutically acceptable salt thereof; ii) an antibody or antigen-binding fragment thereof for binding to PSMA, wherein the antibody or antigen-binding fragment thereof is conjugated to a radionuclide for delivering a radiotherapeutic dose to the cancer; administering to a subject in need of treatment a combination therapy comprising and thereby treating said cancer in said subject. Preferably, said radionuclide is a beta emitter or a beta / gamma emitter. Preferably, the antibody or antigen-binding fragment for binding to PSMA is one described herein, preferably an antibody having the CDRs of antibody J591 described herein. In a preferred embodiment, the antibody comprises a heavy chain with CDR1 as set forth in any of SEQ ID NO:1, SEQ ID NO:17, and SEQ ID NO:244, a CDR2 as set forth in SEQ ID NO:2 or SEQ ID NO:18, and a CDR3 as set forth in SEQ ID NO:3 or SEQ ID NO:19, and a light chain with CDR1 as set forth in SEQ ID NO:33, a CDR2 as set forth in SEQ ID NO:34, and a CDR3 as set forth in SEQ ID NO:35. In a further preferred embodiment, the antibody or antigen-binding fragment thereof comprises an antigen-binding domain comprising the CDRs of the heavy chain variable domain as defined in any of SEQ ID NO:4 or SEQ ID NO:20, and the CDRs of the light chain variable domain as defined in SEQ ID NO:36. In a particular embodiment, the antibody comprises the amino acid sequences set forth in SEQ ID NO:239 and SEQ ID NO:243 herein.

[0023] In any aspect of the invention, the molecular targeted radiotherapeutic agent and the DNA-PKi may be administered sequentially in any order, or may be administered simultaneously. In certain embodiments, the radiotherapeutic agent and the DNA-PKi are administered sequentially in any order. In other embodiments, the radiotherapeutic agent may be administered before the DNA-PKi. In this case, the DNA-PKi may be administered at a later time point on the same day as the radiotherapeutic agent. Preferably, the DNA-PKi is administered 15 days or less after administration of the radiotherapeutic agent, for example, 1 to 15 days or less, preferably 4 to 10 days or less, more preferably 2 to 8 days or less, and most preferably 1 to 5 days or less after administration of the radiotherapeutic agent. In a preferred example, the DNA-PKi is administered 1 day after administration of the radiotherapeutic agent. Administration of DNA-PKi in this context may include a single administration of DNA-PKi, or administration of DNA-PKi over one or more days, for example, over a period as described below.

[0024] It will also be appreciated that the DNA-PKi and the radiotherapeutic agent may be administered via the same or different routes of administration, for example, in a preferred embodiment, the radiotherapeutic agent may be administered intravenously, while the DNA-PKi may be administered orally.

[0025] In a preferred embodiment of any aspect of the invention, the molecular targeted radiotherapeutic agent is administered at a dosage level below the level required for a monotherapy response. This indicates a synergistic effect between the molecular targeted radiotherapeutic agent and the DNA-PKi. Preferably, the molecular targeted radiotherapeutic agent is administered at a dosage of more than 10%, preferably more than 20% less radioactivity compared to a monotherapy response (i.e., treatment involving administration of only the molecular targeted radiotherapeutic agent), preferably 20-50% less radioactivity compared to a monotherapy response. Alternatively, or most preferably, in addition to a dosage of the molecular targeted radiotherapeutic agent, the DNA-PKi is administered at a dosage level below the maximum tolerated dosage level, for example, 90% or less, 85% or less, 80% or less, 75% or less, 60% or less, 65% or less, 60% or less, or 55% or less of the maximum tolerated dosage level, and / or 10% or more, or 20% or more, 30% or more, 40% or more, or 50% or more of the maximum tolerated dosage level of the combination.

[0026] DNA-PKi may be administered at a dose of 0.02 mg to 100 mg / kg body weight, preferably 0.02 mg to 50 mg / kg body weight. Specifically, the daily dose may be 0.02 mg to 100 mg / kg body weight, for example, 1 mg / kg body weight or more, 2 mg / kg body weight or more, 3 mg / kg body weight or more, 4 mg / kg body weight or more, or 5 mg / kg body weight or more, or 25 mg / kg body weight or less, 30 mg / kg body weight or less, 40 mg / kg body weight or less, 45 mg / kg body weight or less, 50 mg / kg body weight or less, 60 mg / kg body weight or less, 70 mg / kg body weight or less, 80 mg / kg body weight or less, 90 mg / kg body weight or less, or 100 mg / kg body weight or less. The DNA-PKi may be administered, for example, at a dose of 1 mg to 800 mg, e.g., 50 mg to 400 mg, 50 mg to 500 mg, 5 mg to 600 mg, more preferably 100 mg to 400 mg, 100 mg to 300 mg, 100 mg to 250 mg, or 100 mg to 200 mg, preferably once a day. Alternatively, the DNA-PKi may be administered at a dose of 150 mg to 400 mg by administration twice a day (bid).

[0027] DNA-Pki may be administered at a dose of 0.01 mg to 1 g, preferably 1 mg to 700 mg, particularly preferably 5 mg to 200 mg per dosage unit.

[0028] In alternative embodiments, the DNA-PKi may be administered daily throughout the course of treatment. In other embodiments, the DNA-PKi may be administered daily starting the day after administration of the radiotherapeutic agent. For example, the DNA-PKi may be administered daily for 7 days or more, 10 days or more, 14 days or more, 21 days or more, 28 days or more, or more.

[0029] In a particularly preferred embodiment, the DNA-PKi is M3814 and the dosage regimen is within the range of 25 mg to 600 mg, 50 mg to 600 mg, 100 mg to 600 mg, 150 mg to 600 mg, 175 mg to 500 mg, 200 mg to 500 mg, 300 mg to 400 mg, 50 mg to 300 mg, 75 mg to 275 mg, 100 mg to 250 mg, or 100 to 200 mg, or one of these combinations. In a particularly preferred embodiment, the aforementioned doses are administered once daily, but may advantageously be administered twice daily (bid). M3814 may be administered, for example, at doses of 75 mg, 100 mg, 125 mg, 150 mg, 175 mg, 200 mg, 250 mg, 275 mg or 300 mg, 350 mg, or 400 mg, preferably once daily but also suitably twice daily, with twice daily doses of 300 mg or more being most preferred.

[0030] One advantage of the present invention, and a difference from the EBRT approach to delivering radiation therapy, is that the radiation therapy agent does not need to be administered every day of the treatment protocol. Thus, in any embodiment of the present invention, the radiation therapy agent may be administered about once a week, about once every two weeks, about once every three weeks, about once every four weeks, or at longer intervals. In a preferred embodiment, the radiation therapy agent is administered twice, 7 days or more, 10 days or more, 14 days or more, 21 days or more, 28 days or more, 35 days or more, 42 days or more, 49 days or more, 56 days or more, or three times, 7 days or more, 10 days or more, 14 days or more, 21 days or more, 28 days or more, 35 days or more, 42 days or more, 49 days or more, 56 days or more, or more. It will be understood that additional administrations of the radiation therapy agent may be required. In certain embodiments, successful treatment may require only a single administration of the radiotherapeutic agent, and thus the present invention contemplates treatment regimens in which only a single administration of the radiotherapeutic agent is administered in conjunction with administration of the DNA-PKi described herein.

[0031] In some embodiments, the treatment comprises one or more treatment cycles, where the treatment cycle comprises administration of a radiotherapy agent on the first day of the cycle, followed by administration of a DNA-PKi for 7 days or more, e.g., 14 days, starting on the second, third, fourth, fifth, sixth, or seventh day of the cycle, resulting in a cycle of, e.g., 14 or 15 days. In some embodiments, the treatment cycle comprises administration of a radiotherapy agent on the first day of the cycle, followed by administration of a DNA-PKi for 20 days or more, starting on the second, third, fourth, fifth, sixth, or seventh day of the cycle, resulting in a cycle of, e.g., 21 or 22 days. It is preferred to start DNA-PKi treatment on the second day of the cycle. The treatment may comprise, for example, one, two, three, or more such treatment cycles, optionally with a break in treatment.

[0032] In embodiments in which the treatment comprises two or more treatment cycles, the subsequent treatment cycle may begin immediately after completion of the first treatment cycle (e.g., several days after completion of the first treatment cycle), or may begin several days or weeks after completion of the first treatment cycle, following a period during which no radiotherapeutic agent or DNA-PKi is administered (i.e., a treatment break). The treatment break may be one day or more, or one week or more, two weeks or more, three weeks or more.

[0033] In some embodiments, the treatment can include two or more treatment cycles, or three or more treatment cycles, where each treatment cycle includes administration of a radiotherapeutic agent on the first day of the cycle followed by administration of a DNA-PKi on subsequent days of the treatment cycle (e.g., starting on day 2, day 3, or day 4), and includes administration of a DNA-PKi until at least day 7, at least day 14, or at least day 21 of the treatment cycle, or includes administration of a DNA-PKi for a period of 7 days or more, 14 days or more, or 21 days or more. The period between the end of the first treatment cycle and the start of the second treatment cycle (i.e., the period during which the DNA-PKi and radiotherapeutic agent are not administered) can be 7 days or more, 14 days or more, 21 days or more, 28 days or more, 35 days or more, 42 days or more, 49 days or more, 56 days or more, or more.

[0034] For example, a treatment cycle may include administration of a radiotherapy agent on the first day of the cycle, followed by administration of a DNA-PKi agent starting on the second day of the cycle for a period of 7 days or more, 14 days or more, or 20 days or more, for example, a 21-day cycle. The start of the second treatment cycle can be delayed for a period of 2 days or more, 5 days or more, 7 days or more, 14 days or more, 21 days or more, 28 days or more, 35 days or more, 42 days or more, 49 days or more, 56 days or more, or more, so that the start of the second treatment cycle is, for example, 56 days or more after the first administration of the radiotherapy agent. In such an embodiment, the second treatment cycle can substantially repeat the first treatment cycle, for example, starting administration of a radiotherapy agent on the first day of the second treatment cycle, followed by administration of a DNA-PKi agent starting on the second day of the second cycle and administered for 7 days or more, 14 days or more, or 21 days or more, so that the overall treatment period is, for example, 77 days or more.

[0035] In another example, a treatment cycle can include administration of a radiotherapeutic agent on the first day of the cycle, followed by administration of a DNA-PKi agent beginning on day 4 of the cycle and continuing for a period of 7 days or more, 14 days or more, 17 days or more, or 20 days or more, for example, a 21-day cycle. The start of a second treatment cycle can be delayed for 2 days or more, 5 days or more, 7 days or more, 14 days or more, 21 days or more, 28 days or more, 35 days or more, 42 days or more, 49 days or more, 56 days or more, 63 days or more, or more, such that the start of the second treatment cycle is, for example, at least 85 days after the first administration of the radiotherapeutic agent (e.g., if the first dose is on day 1, DNA-PKi treatment is from day 4 to day 21, and optionally, the treatment break is from day 22 to day 84). In such embodiments, the second treatment cycle can substantially repeat the first treatment cycle, e.g., by administering the radiotherapeutic agent beginning on day 1 of the second treatment cycle, followed by administration of the DNA-PKi beginning on day 4 of the second cycle, and administered for 7 days or more, 14 days or more, 17 days or more, or 20 days or more, resulting in an overall treatment period of, e.g., 84 days or more.

[0036] In other embodiments, the treatment may include three or more treatment cycles, for example, each treatment cycle including administration of a radiotherapeutic agent on the first day of the cycle, followed by administration of a DNA-PKi on subsequent days for 7 or more days or 14 or more days. Treatment breaks may be included between the end of the first treatment cycle and the start of the second treatment cycle, as well as between the end of the second treatment cycle and the start of the third treatment cycle, and optionally each treatment break is 2 or more days, 5 or more days, 7 or more days, 10 or more days, 14 or more days, or 21 or more days, 28 or more days, 35 or more days, 42 or more days, 49 or more days, 56 or more days, 63 or more days, or more, and most preferably the treatment break is 7 days or more.

[0037] Suitable time schedules for treatment or treatment cycles are also described in the Examples. These time schedules are also suitable for other DNA-PKi and radiotherapeutic drug combinations, and for other types of cancers or tumors in general.

[0038] The combination therapy of the present invention can be used alone or in combination with other treatment modalities, such as surgery, external beam radiotherapy, chemotherapy, other radionuclides, or tissue thermoregulation, etc. This forms another preferred embodiment of the method of the present invention, whereby the formulation / medicament may correspondingly comprise at least one additional therapeutically active agent, such as another radiopharmaceutical or a chemotherapeutic agent.

[0039] In any aspect of the invention, optionally, the therapy may further comprise iii) an additional anti-cancer therapy selected from the group consisting of an immune checkpoint modulator, a chemotherapeutic agent, a radiosensitizer, and EBRT.

[0040] In any embodiment, the additional anti-cancer therapy may include an immune checkpoint modulating agent, which may be an immune checkpoint inhibitor selected from an inhibitor of PD-1, PD-L1, and CTLA-4, or any other immune checkpoint inhibitor described herein.

[0041] Optionally, the immune checkpoint inhibitor is an inhibitor of PD-1 selected from pembrolizumab, nivolumab, cemiplimab, spartalizumab, canrelizumab, sintilimab, tislelizumab, toripalimab, dostarimab, INCMGA00012, AMP-224, and AMP-514.

[0042] Optionally, the immune checkpoint inhibitor is an inhibitor of PD-L1 selected from atezolizumab, avelumab, durvalumab, KN035, CK-301, AUNP12, CA-170, and BMS-986189.

[0043] The immune checkpoint inhibitor can be an inhibitor of CTLA-4 selected from ipilimumab and tremelimumab.

[0044] In any embodiment, the other anti-cancer therapy is a chemotherapeutic agent, as described in more detail herein.

[0045] In any embodiment of the first aspect of the invention, the DNA-PKi is M3814, N-methyl-8-[(2S)-1-{[2'-methyl(4',6'- 2 H2)-[4,5'-bipyrimidin]-6-yl]amino}propan-2-yl]quinoline-4-carboxamide, 7,9-dihydro-7-methyl-2-[(7-methyl[1,2,4]triazolo[1,5-a]pyridin-6-yl)amino]-9-(tetrahydro-2H-pyran-4-yl)-8H-purin-8-one (AZD7648), 4-ethyl-N-[4-[2-(4-morpholinyl)-4-oxo-4H-1-benzopyran-8-yl]-1-dibenzothienyl]-1-piperazineacetamide (KU-0060648), 2-(4-morpholinyl)-4H-naphtho[1,2-b]pyran-4-one (NU7026), 8-(4-dibenzothienyl) enyl)-2-(4-morpholinyl)-4H-1-benzopyran-4-one (NU7441, KU-57788), 3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidin-2-yl]-phenol (PI-103), 2-methyl-5-nitro-2-[(6-bromoimidazo[1,2-a]pyridin-3-yl)methylene]-1-methylhydrazide-benzenesulfonic acid, monohydrochloride (PIK-75HCl), 1-cyclopentyl-3-(1H-pyrrolo[2,3-b]pyridin-5-yl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (PP121), SF2523, and analogs thereof.

[0046] Preferably, the DNA-PKi is (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharma- ceutically acceptable salt thereof.

[0047] According to an optional aspect of the present invention there is provided (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814), or a pharma- ceutically acceptable salt thereof, for use in the methods of treatment described herein.

[0048] According to any aspect of the present invention, a method for treating i) an antibody or antigen-binding fragment thereof for binding to CAIX, preferably as described herein, wherein said antibody is conjugated to an antibody radionuclide; or ii) an antibody or antigen-binding fragment thereof for binding to PSMA, preferably as described herein, wherein the antibody is conjugated to a radionuclide; is provided.

[0049] Still further, according to a second aspect of the present invention there is provided an antibody or antigen-binding fragment thereof for binding to CAIX, preferably (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharma- ceutically acceptable salt thereof for use in the methods of treatment described herein when administered in therapy in combination with an antibody or antigen-binding fragment thereof for binding to CAIX, wherein the antibody is conjugated to a radionuclide.

[0050] Still further, according to a second aspect of the invention there is provided an antibody or antigen-binding fragment thereof for binding to PSMA, preferably (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharma- ceutically acceptable salt thereof for use in the methods of treatment described herein when administered in therapy in combination with an antibody as described herein, wherein the antibody is conjugated to a radionuclide.

[0051] According to any aspect of the invention, in the manufacture of a medicament for use in the methods of treatment described herein, i) an antibody or antigen-binding fragment thereof for binding to CAIX, preferably as described herein, wherein said antibody is conjugated to a radionuclide; or ii) an antibody or antigen-binding fragment thereof for binding to PSMA, preferably as described herein, wherein the antibody is conjugated to a radionuclide; The use of

[0052] According to a second aspect of the invention there is also provided the use of (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814), or a pharma- ceutically acceptable salt thereof, in the manufacture of a medicament or kit of parts for use in the treatment of a disease or condition as described herein, wherein said treatment comprises the administration of an antibody or antigen-binding fragment thereof for binding to CAIX, preferably as described herein, wherein said antibody is conjugated to a radionuclide.

[0053] Still further according to a second aspect of the present invention there is provided the use of (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814), or a pharma- ceutically acceptable salt thereof, in the manufacture of a medicament or kit of parts for use in the treatment of a disease or condition as described herein, wherein said treatment comprises the administration of an antibody or antigen-binding fragment thereof for binding to PSMA, preferably as described herein, wherein said antibody is conjugated to a radionuclide.

[0054] The present invention also relates to a method for producing a first medicament, comprising: i) an antibody or antigen-binding fragment thereof for binding to CAIX, preferably as described herein, which is conjugated to a radionuclide; or ii) an antibody or antigen-binding fragment thereof for binding to PSMA, preferably as described herein, wherein the antibody or antigen-binding fragment thereof is conjugated to a radionuclide; Use of, and Use of DNA-PKi, preferably (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814), in the manufacture of a second medicament. wherein the first and second medicaments are administered according to any of the methods of treatment described herein.

[0055] The present invention also provides a kit for use in any of the methods of the present invention described herein.Preferably, the kit comprises an antibody as described herein together with a DNA-PKi, preferably (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharma- ceutically acceptable salt thereof, and optionally includes instructions for use according to the methods of the present invention.

[0056] As used herein, unless the context requires otherwise, the term "comprise" and variations of this term, such as "comprising," "including," and "comprised," are not intended to exclude additional additives, components, integers, or steps.

[0057] Other aspects of the invention and other embodiments of the aspects described in the preceding paragraphs will become apparent from the following description, which is given by way of example only, and with reference to the accompanying drawings, in which: [Brief description of the drawings]

[0058] [Figure 1] 177Lu-anti-CAIX antibody SPECT imaging in metastatic renal cell carcinoma SK-RC-52 xenograft model. [Diagram 2] Combination treatment with 177Lu-anti-CAIX antibody + M3814. Mean tumor volume (mm3) over 100 days after 14 days of treatment is shown. Percentage change in tumor volume is also shown. Kaplan-Meier survival curves for the 6MBq dose shown on day 100 of the study. [Diagram 3] 177Lu-anti-PSMA antibody SPECT imaging in LNCaP (PSMAhigh) xenograft model. [Figure 4] Combination treatment with 177Lu-anti-PSMA antibody + M3814. Mean tumor volume (mm3) over 100 days after 14 days of treatment is shown. Percent change in tumor volume is also shown. Kaplan-Meier survival curves up to day 120. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0059] It will be understood that the invention disclosed and defined herein extends to all alternative combinations of two or more of the individual features mentioned or apparent from the text or drawings, all of these different combinations constituting various alternative aspects of the invention.

[0060] Reference will now be made in detail to certain embodiments of the invention. While the invention will be described in conjunction with the embodiments, it will be understood that it is not intended to limit the invention to those embodiments. On the contrary, the invention is intended to cover all alternatives, modifications, and equivalents which may be included within the scope of the present invention as defined by the claims.

[0061] The present invention relates to a new therapeutic approach involving the combination of an inhibitor of DNA-PK with a molecularly targeted radiotherapeutic agent capable of internalization and / or retention in the blood circulation of a subject.

[0062] Conventional treatment of cancer using DNA damage repair inhibitors (DDRi) has involved administration of DDRi and external beam radiation (EBRT) therapy simultaneously. Such treatment, although effective, results in increased toxicity of the administered DDRi. Furthermore, this treatment method is complex and requires frequent outpatient visits to receive EBRT. For example, a course of EBRT usually involves several daily treatments (fractions) over a period of days to weeks, during which the patient receives radiation therapy. Although the radiation beam targets specific areas of the body, including the tumor, the radiation is non-specific, and healthy tissue is also exposed to radiation during the course of treatment.

[0063] The inventors have recognised the advantages of substituting molecularly targeted radiotherapy for EBRT, particularly where the radiotherapeutic agent is capable of being internalised and retained in the patient's blood circulation, as is the case for macromolecular (e.g. antibody) radiotherapeutic agents.

[0064] The inventors have demonstrated that successful combined radiotherapy and DNA-PKi treatment can be achieved with just one dose of radioimmunoconjugate. This provides a major advantage in clinical practice, where patients only need to receive a single dose of radiotherapy via injection, rather than visiting the clinic frequently over a long period of time to receive EBRT. Furthermore, the use of molecular targeted radiation delivers the radiotherapy dose directly to the tissues that require treatment, rather than requiring an external radiation beam to pass through healthy tissues. Thus, molecular targeted radiation reduces unnecessary exposure of healthy tissues to radiation.

[0065] Furthermore, the use of macromolecular radioimmunoconjugates offers significant advantages compared to the use of small molecules or peptides that can bind to the same molecular targets and be used to deliver doses of radiotherapeutic drugs. Without wishing to be bound by theory, the inventors believe that the rapid urinary excretion of radiolabeled small molecules or peptides means that a smaller radiation dose can be administered to the patient, compared to the slower hepatic degradation of larger molecules such as antibodies. This has the advantage of reducing the risk of nephrotoxicity that can occur with the use of radioconjugates that are excreted via the kidney.

[0066] Furthermore, radioimmunoconjugates can be designed to deliver radiotherapy in a significantly more targeted manner compared to EBRT and to be functionally specific to tumor-expressed antigens. For example, small molecules and peptides used to target the same antigen (e.g., PSMA) usually also target non-cancerous tissues that express the same antigen (in the case of PSMA, lacrimal / salivary glands, ganglia, and small intestine), resulting in unpleasant side effects. Thus, the use of antibody-based methods for the delivery of radiotherapy significantly reduces the undesirable side effects resulting from on-target extratumoral binding.

[0067] Furthermore, the antibodies and fragments thereof can be modified to increase or decrease their persistence in the blood circulation (eg, by modifying the binding site for FcRn, decreasing serum half-life).

[0068] Thus, the inventors believe that the specific combination of antibody-delivered radiotherapy with DNA-PKi offers significant advantages in terms of reducing radiation doses and improving clinical settings for the delivery of radiotherapy, in some cases requiring only a single administration of molecularly targeted radiation.

[0069] The inventors also believe that the use of DNA-PKi in combination with radioimmunotherapeutic agents conjugated to long-range radioisotopes (such as beta-emitting radionuclides) offers additional advantages over, for example, the use of shorter-range particles (such as alpha-emitting radionuclides). Without wishing to be bound by theory, it is believed that the crossfire effect resulting from beta-emitters may result in improved therapeutic outcomes, especially in patients with large tumor masses.

[0070] Overview and definition Throughout this specification, unless expressly stated otherwise or the context requires otherwise, references to a single step, composition of matter, group of steps, or composition of matter shall be construed to include one and more (i.e., one or more) of those steps, compositions of matter, group of steps, or compositions of matter. Thus, as used herein, the singular forms "a," "an," and "the" include plural aspects, and vice versa, unless the context clearly dictates otherwise. For example, reference to "a" includes the singular and more than one, reference to "an" includes the singular and more than one, reference to "the" includes the singular and more than one, etc.

[0071] Those skilled in the art will appreciate that the present invention is susceptible to variations and modifications other than those specifically described. It is to be understood that the present invention includes all such variations and modifications. The present invention also includes all of the steps, functions, compositions, and compounds referred to or indicated herein, individually or collectively, and includes any and all combinations of the steps or functions or any two or more of them.

[0072] One skilled in the art will recognize many methods and materials similar or equivalent to those described herein, which could be used in the practice of the present invention, and the present invention is in no way limited to the methods and materials described.

[0073] All patents and publications mentioned herein are incorporated by reference in their entirety.

[0074] The present invention is not to be limited in scope by the specific examples described herein, which are for the purposes of illustration only. Functionally equivalent products, compositions and methods are clearly within the scope of the invention.

[0075] Any example or embodiment of the invention in this specification shall be construed to apply mutatis mutandis to any other example or embodiment of the invention, unless expressly stated otherwise.

[0076] Unless otherwise defined, all technical and scientific terms used herein are intended to have the same meaning as commonly understood by one of ordinary skill in the art (e.g., in diagnostic techniques, radioimaging, cell culture, molecular genetics, immunology, immunohistochemistry, protein chemistry, and biochemistry).

[0077] The term "and / or", e.g., "X and / or Y", shall be understood to mean either "X and Y" or "X or Y" and shall be interpreted as explicitly endorsing both meanings or either meaning.

[0078] As used herein, the term "derived from" shall be construed to indicate that a particular integer may be obtained from a particular source, although not necessarily directly from that source.

[0079] As used herein, the term "antigen-binding domain" refers to the region of an antibody capable of specifically binding to an antigen, i.e., the V H Or V Lor an Fv comprising both a VH and a VL. The antigen-binding domain need not be an entire antibody, for example it may be in separate form (e.g. a domain antibody) or in another form, such as those described herein, for example an scFv.

[0080] In this disclosure, the term "antibody" includes proteins capable of specifically binding to one or a small number of closely related antigens by means of an antigen-binding domain contained within the Fv. The term includes four-chain antibodies (e.g., two light chains and two heavy chains), recombinant antibodies, or engineered antibodies (e.g., chimeric antibodies, humanized antibodies, human antibodies, CDR-grafted antibodies, primatized antibodies, deimmunized antibodies, synthetic humanized antibodies, half antibodies, bispecific antibodies).

[0081] Antibodies generally contain a constant domain, which can be arranged in a constant region or constant fragment or crystallizable fragment (Fc). Exemplary forms of antibodies contain a four-chain structure as their basic unit. Full-length antibodies contain two covalently bound heavy chains (about 50 kD to about 70 kD) and two light chains (about 23 kDa each). The light chains usually contain a variable region (if present) and a constant domain, and in mammals are either kappa or lambda light chains. The heavy chains usually contain a variable region and one or two constant domains linked to other constant domains by a hinge region. Mammalian heavy chains are one of the alpha, delta, epsilon, gamma, or mu types. Each light chain is also covalently bound to one of the heavy chains. For example, the two heavy chains and the heavy and light chains are bound by interchain disulfide bonds and non-covalent interactions. The number of interchain disulfide bonds can vary between different types of antibodies. Each chain consists of an N-terminal variable region (V H Or V L , each about 110 amino acids long) and one or more constant domains at the C-terminus. L ) is the first constant domain of the heavy chain (C H1) and are disulfide bonded. The light chain variable region is aligned with the heavy chain variable region. The antibody heavy chain has two or more additional C H Domain (e.g., C H2 , C H3 etc.), C H1 Constant domain and C H2 The constant domain may include a hinge region. The antibody may be of any type (e.g., IgG, IgE, IgM, IgD, IgA, and IgY), class (e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2), or subclass. In some examples, the antibody is a murine (mouse or rat) antibody or a primate (e.g., human) antibody. In some examples, the antibody heavy chain lacks a C-terminal lysine residue. In some examples, the antibody is humanized, synthetic humanized, chimeric, CDR-grafted, or deimmunized.

[0082] The terms "full length antibody," "intact antibody," or "whole antibody" are used interchangeably to refer to an antibody in a substantially intact form, as opposed to an antigen-binding fragment of an antibody. Specifically, whole antibodies include those having heavy and light chains, including the Fc region. The constant domains may be wild-type sequence constant domains (e.g., human wild-type sequence constant domains) or amino acid sequence variants thereof.

[0083] As used herein, a "variable region" refers to a portion of the light and / or heavy chain of an antibody as defined herein that is capable of specifically binding to an antigen and includes the amino acid sequences of the complementarity determining regions (CDRs), i.e., CDR1, CDR2, and CDR3, and framework regions (FRs). For example, a variable region includes three CDRs together with three or four FRs (e.g., FR1, FR2, FR3, and optionally FR4). H refers to the variable region of the heavy chain. L refers to the variable region of the light chain.

[0084] As used herein, the term "complementarity determining region" (also known as CDR, i.e., CDR1, CDR2, and CDR3) refers to the amino acid residues of an antibody variable region whose presence is a major contributor to specific antigen binding. Each variable region domain (V H Or V L ) typically has three CDRs identified as CDR1, CDR2, and CDR3. H The CDRs of are also referred to herein as CDR H1, CDR H2, and CDR H3, respectively, and CDR H1 is V H CDR1 corresponds to V H CDR2 corresponds to V H Similarly, V L The CDRs of are referred to herein as CDR L1, CDR L2, and CDR L3, respectively, and CDR L1 is V L CDR1 corresponds to V L CDR2 corresponds to V LIn one example, the amino acid positions assigned to the CDRs and FRs are defined according to the Kabat Sequences of Proteins of Immunological Interest, National Institutes of Health, Bethesda, Md., 1987 and 1991 (also referred to herein as the "Kabat numbering system"). In another example, the amino acid positions assigned to the CDRs and FRs are defined according to the modified Chothia numbering scheme (http: / / www.bioinfo.org.uk / mdex.html). The present invention is not limited to FRs and CDRs defined by the Kabat numbering system, but includes all numbering systems, such as the standard numbering systems, or the numbering systems of Chothia and Lesk J. Mol. Biol. 196: 901-917, 1987, Chothia et al., Nature 342: 877-883, 1989, and / or Al-Lazikani et al., J. Mol. Biol. 273: 927-948, 1997, the numbering system of Honnegher and Plukthun J. Mol. Biol. 309: 657-670, 2001, or the IMGT system discussed in Giudicelli et al., Nucleic Acids Res. 25: 206-211 1997.

[0085] In one example, the CDRs are defined according to the Kabat numbering system. Optionally, the heavy chain CDR2 according to the Kabat numbering system does not include the five C-terminal amino acids listed herein, or any one or more of those amino acids are substituted with another naturally occurring amino acid. In this regard, Padlan et al., FASEB J., 9: 133-139, 1995, demonstrated that the five C-terminal amino acids of the heavy chain CDR2 are not normally involved in antigen binding.

[0086] "Framework regions" (FR) are those variable region residues other than the CDR residues. The FRs of VH are also referred to herein as FR H1, FR H2, FR H3, and FR H4, respectively; FR H1 is the V H FR1 corresponds to V, FR H2 corresponds to V H FR2 corresponds to V, FR H3 corresponds to V H FR3 corresponds to V, FR H4 corresponds to V H Similarly, V L The FRs are referred to herein as FR L1, FR L2, FR L3, and FR L4, respectively, and FR L1 is V L FR1 corresponds to V, and FR L2 corresponds to V L FR2 corresponds to V, and FR L3 corresponds to V L FR3 corresponds to V, FR L4 corresponds to V L Compatible with FR4.

[0087] As used herein, the term "Fv" refers to a V polypeptide, whether composed of multiple polypeptides or a single polypeptide. L and V H The term "antigen-binding domain" is intended to mean any protein that can specifically bind to an antigen and that can associate with and form a complex having an antigen-binding domain. H and V L The V may be a single polypeptide chain or different polypeptide chains. Furthermore, an Fv of the invention (as well as any protein of the invention) may have multiple antigen binding domains that may or may not bind to the same antigen. This term shall be understood to encompass fragments derived directly from antibodies, as well as proteins corresponding to such fragments produced using recombinant means. In some instances, the V H is the heavy chain constant domain (C H ) 1 and / or V L is the light chain constant domain (C LExemplary Fv-containing polypeptides or proteins include a Fab fragment, a Fab' fragment, a F(ab') fragment, an scFv, a diabody, a triabody, a tetrabody, or a higher order complex, or any of the foregoing linked to a constant region or domain thereof (e.g., a CH2 domain or a CH3 domain, e.g., a minibody).

[0088] A "Fab fragment" consists of a monovalent antigen-binding fragment of an immunoglobulin and can be produced by digestion of a whole antibody with the enzyme papain to produce a fragment consisting of an intact light chain and a portion of the heavy chain, or can be produced using recombinant means. An "Fab' fragment" of an antibody can be produced by treating a whole antibody with pepsin, followed by reduction, to produce an intact light chain and a portion of the heavy chain. H and a portion of the heavy chain containing a single constant domain. Two Fab' fragments are obtained per antibody treated in this way. Fab' fragments can also be produced by recombinant means. An "F(ab')2 fragment" of an antibody consists of a dimer of two Fab' fragments linked by two disulfide bonds and can be obtained by treating the whole antibody molecule with the enzyme pepsin without subsequent reduction. A "Fab2" fragment is a recombinant fragment containing two Fab fragments linked, for example, using a leucine zipper or CH3 domain. A "single-chain Fv" or "scFv" is a recombinant molecule containing an antibody variable fragment (Fv) in which the variable region of the light chain and the variable region of the heavy chain are covalently linked by a suitable flexible polypeptide linker.

[0089] As used herein, the term "bind" in reference to the interaction of an antigen-binding protein or antigen-binding domain thereof with an antigen means that the interaction is dependent on the presence of a particular structure (e.g., an antigenic determinant or epitope) on the antigen. For example, an antibody recognizes and binds to a particular protein structure, not the entire protein. If an antibody binds to epitope "A", in a reaction containing labeled "A" and a protein, the presence of a molecule containing epitope "A" (or free unlabeled "A") will reduce the amount of labeled "A" bound to the antibody.

[0090] As used herein, the terms "specific binding" or "specifically binds" shall be taken to mean that an antigen binding protein of the invention reacts or associates with a particular antigen or a cell expressing it with a greater frequency, more rapidity, longer duration, and / or with greater affinity than it reacts with another antigen or cell. Generally, but not necessarily, reference to binding shall mean specific binding, and each term shall be understood to provide explicit support for the other term.

[0091] As used herein, the term "does not detectably bind" shall be understood to mean that an antigen binding protein, e.g., an antibody, binds to a candidate antigen at a level less than 10%, or less than 8%, or less than 6%, or less than 5% above background. Background may be the level of binding signal detected in the absence of protein and / or in the presence of a negative control protein (e.g., an isotype control antibody), and / or the level of binding detected in the presence of a negative control antigen. The level of binding is detected using a biosensor assay (e.g., Biacore) in which the antigen binding protein is immobilized and contacted with the antigen.

[0092] As used herein, the term "does not bind significantly" shall be understood to mean that the level of binding of an antigen binding protein of the invention to a polypeptide is not statistically significantly higher than the background, e.g., the level of binding signal detected in the absence of the antigen binding protein and / or in the presence of a negative control protein (e.g., an isotype control antibody), and / or the level of binding detected in the presence of a negative control polypeptide. The level of binding is detected using a biosensor assay (e.g., Biacore or Blitz) in which the antigen binding protein is immobilized and contacted with the antigen.

[0093] As used herein, the term "epitope" (also known as "antigenic determinant") shall be understood to mean the region of an antigen to which an antigen binding protein binds, including the antigen binding domain of an antibody. Unless otherwise specified, the term is not necessarily limited to the particular residues or structures contacted by the antigen binding protein. For example, the term includes the region spanning the amino acids contacted by the antigen binding protein, as well as 5-10 (or more) amino acids, or 2-5, or 1-3 amino acids outside of this region. In some instances, an epitope includes a series of discontinuous amino acids that are positioned close to each other when the antigen binding protein is folded, i.e., a "conformational epitope." One of skill in the art will also know that the term "epitope" is not limited to peptides or polypeptides. For example, the term "epitope" includes chemically active surface groups of molecules, such as sugar, phosphoryl, or sulfonyl side chains, and in certain instances may have specific three-dimensional structural characteristics and / or specific charge characteristics.

[0094] As used herein, the terms "preventing," "prevent" or "prevention" include administering an antigen binding protein of the invention, thereby arresting or preventing the onset of at least one symptom of a condition. The term also encompasses treatment to prevent or hinder recurrence in a subject in remission.

[0095] As used herein, the terms "treating," "treat" or "treatment" include administering an antigen binding protein described herein, thereby reducing or eliminating at least one symptom of a particular disease or condition.

[0096] As used herein, the term "subject" shall be taken to mean any animal, e.g., a mammal, including a human. Exemplary subjects include, but are not limited to, humans and non-human primates. For example, the subject is a human.

[0097] Inhibitors of cell and DNA damage repair In accordance with the methods of the present invention, combination therapies are provided that include a DNA-PK inhibitor (DNA-PKi) and a radioimmunoconjugate, which have been shown to provide unexpected improvements in the treatment of cancer.

[0098] DNA-dependent protein kinase (DNA-PK) is a serine / threonine protein kinase that is activated in conjunction with DNA. Biochemical and genetic data indicate that DNA-PK consists of (a) a catalytic subunit called DNA-PKcs, and (b) two regulatory components (Ku70 and Ku80). Functionally, DNA-PK is a key component of the repair of DNA double-strand breaks (DSBs) on the one hand, and somatic or V(D)J recombination on the other hand. Furthermore, DNA-PK and its components are associated with many other physiological processes, such as the regulation of chromatin structure and telomere maintenance (Smith & Jackson (1999) Genes and Dev 13: 916; Goytisolo et al. (2001) Mol. Cell. Biol. 21: 3642; Williams et al. (2009) Cancer Res. 69: 2100).

[0099] The present invention relates to the use of compounds capable of inhibiting the activity of DNA-PK. As used herein, the term "inhibition" or "inhibiting" refers to any reduction in activity based on the action of certain compounds described herein, in that they are able to interact with the target molecule in a way that allows recognition, binding and blocking. The compounds are distinguished by a high affinity for at least one serine / threonine protein kinase, ensuring reliable binding and preferably complete blocking of kinase activity. The compounds are particularly preferably monospecific, to ensure exclusive and direct recognition of the selected kinase. The term "recognition" here refers to any type of interaction between the compound and the target molecule, in particular covalent or non-covalent, such as covalent bonds, hydrophobic / hydrophilic interactions, van der Waals forces, ionic attraction, hydrogen bonds, ligand / receptor interactions, base pairs of nucleotides, or interactions between epitopes and antibody binding sites.

[0100] As used herein, DNA-PKi should be understood to include molecules that substantially inhibit DNA-PK. Preferably, DNA-PKi has an IC of less than 500 nM, preferably less than 250 nM, less than 100 nM, less than 50 nM, less than 10 nM, or less than 1 nM at an ATP concentration close to the Km (10 μM). 50 It inhibits DNA-PK at IC (half inhibitory concentration). 50 The value may be determined, for example, by biochemical assays, which are described in more detail below.

[0101] Preferably, the DNA-PKi has a selectivity (i.e., specificity) for DNA-PK that is greater than 5-fold, greater than 10-fold, greater than 15-fold, or greater than 20-fold, particularly over other related kinases, such as kinases of the PI3K family. Most preferably, the DNA-PKi has a selectivity (specificity) for DNA-PK that is greater than 10-fold over related serine / threonine, tyrosine, or lipid kinases. In preferred embodiments, the DNA-PKi does not substantially inhibit ATR, ATM, and mTOR. In preferred embodiments, the selectivity of the DNA-PKi for PI3K alpha, PI3K beta, PI3K gamma, PI3K delta, mTOR, ATM, and / or ATR is greater than 10-fold, more preferably greater than 50-fold or greater than 100-fold. Selectivity is measured using biochemical IC 50 value (e.g., IC 50 PI3K Alpha / IC 50 It can be calculated based on the DNA-PK ratio.

[0102] The ability of a molecule to inhibit DNA-PK can be measured by known methods in the prior art. For example, such methods are described in WO 2014 / 183850, which is incorporated herein by reference. Briefly, inhibition can be measured via changes in kinase activity levels. Measuring kinase activity is a technique well known to those skilled in the art. Common test systems for determining kinase activity using substrates such as histones (Alessi et al. (1996) FEBS Lett. 399(3): 333) or basic myelin proteins are described in the literature (Campos-Gonzalez & Glenney (1992) JBC 267: 14535). Different assay systems can be used to identify kinase inhibitors. In the scintillation proximity assay (Sorg et al. (2002) J Biomolecular Screening 7: 11) and the flashplate assay, the radioactive phosphorylation of a protein or peptide as a substrate is measured using ATP. In the presence of an inhibitory compound, the detectable radioactive signal is reduced or not detected at all. In addition, homogeneous time-resolved fluorescence resonance energy transfer (HTR-FRET) and fluorescence polarization (FP) techniques are useful as assay methods (Sills et al. (2002) J Biomolecular Screening 191). Another non-radioactive ELISA method uses a specific phospho-antibody (phospho-AB). Phospho-AB binds only phosphorylated substrates. This binding can be detected by chemiluminescence using a peroxidase-conjugated anti-sheep secondary antibody.

[0103] The sensitivity of a particular cell to treatment with the compounds of the present invention can be determined by in vitro testing. Usually, a culture of cells is incubated with the compounds of the present invention at various concentrations for a period sufficient to allow the active agent to induce cell death or inhibit cell proliferation, cell vitality or migration, usually from about 1 hour up to 9 days. For in vitro testing, cultured cells from a biopsy sample can be used. The amount of cells remaining after treatment is then determined. In vitro use is particularly carried out on samples of mammalian species with cancer, tumors or tumor metastases. The host or patient may belong to any mammalian species, for example primate species, in particular humans, but also rodents (including mice, rats and hamsters), rabbits, horses, cows, dogs, cats, etc. Animal models are interesting for experimental studies and provide models for the treatment of human diseases.

[0104] More specifically, biochemical methods for assessing DNA-PK activity can be as described in Kashishian et al. (2003) Molecular Cancer Therapeutics 1257. Briefly, the assay can be performed in streptavidin-coated 348-well microtiter flash plates. For this purpose, 1.5 μg of DNA-PK / protein complex and 100 ng of biotinylated substrate, e.g., PESQEAFADLWKK-biotin-NH2 ("biotin-DNA-PK peptide"), can be incubated with 500 ng of DNA from calf thymus, 0.1 μCi of 33P-ATP, and 1.8% DMSO per well in a total volume of 36.5 μl (34.25 mM HEPES / KOH, 7.85 mM Tris-HCl, 68.5 mM KCl, 5 μM ATP, 6.85 mM MgCl2, 0.5 mM EDTA, 0.14 mM EGTA, 0.69 mM DTT, pH 7.4) with and without test compound for 90 min at room temperature. The reaction is then stopped with 50 μl / well of 200 mM EDTA. After a further 30 min of incubation at room temperature, the liquid is removed. Each well is washed 3 times with 100 μl of 0.9% saline. The non-specific reaction (blank value) is determined using 10 μM of a native kinase inhibitor. Radioactivity measurements can be carried out using a TopCount. IC50 values ​​were calculated with RS1.

[0105] Cell-based assays for assessing DNA-PK activity can also be used. In one example, HCT116 cells are cultured in MEM alpha medium containing 10% fetal bovine serum and 2 mM glutamine at 37° C. and 10% CO2. The cells are detached from the base of the culture vessel using trypsin / EDTA, centrifuged in a centrifuge tube, taken up in fresh medium, and the cell density is determined. 100,000 cells are seeded in 1 ml of culture medium per cavity of a 24-well cell culture plate and cultured overnight. The next day, 10 μM bleomycin (a DNA intercalator and inducer of DNA double-strand breaks) and test substances in fresh culture medium are added to the cells, and they are cultured for another 6 hours. Cell lysis is then performed and the cell lysate is added to a blocked 96-well ELISA plate coated with DNA-PK specific antibodies (Sigma-Aldrich WH0005591M2: total DNA-PK, Abcam ab18192 or Epitomics EM09912: phospho-serine 2056 DNA-PK) and incubated overnight at 4°C. The 96-well ELISA plate is then treated with detection antibodies (Abcam ab79444: total DNA-PK) and streptavidin-HRP complex. The enzymatic reaction is developed using chemiluminescence reagents and chemiluminescence can be measured using Mithras LB940. The signal from the phospho-DNA-PK specific antibody is normalized to the signal from the antibody against the total protein DNA-PKc. IC 50 Values ​​or percentage values ​​were determined by referencing the signal level of the bleomycin-treated vehicle control group (100% of control). A DMSO control can be used as a blank.

[0106] Non-limiting examples of DNA-PK inhibitors include (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814), N-methyl-8-[(2S)-1-{[2'-methyl(4',6'- 2H2)-[4,5'-bipyrimidin]-6-yl]amino}propan-2-yl]quinoline-4-carboxamide, 7,9-dihydro-7-methyl-2-[(7-methyl[1,2,4]triazolo[1,5-a]pyridin-6-yl)amino]-9-(tetrahydro-2H-pyran-4-yl)-8H-purin-8-one (AZD7648), 4-ethyl-N-[4-[2-(4-morpholinyl)-4-oxo-4H-1-benzopyran-8-yl]-1-dibenzothienyl]-1-piperazineacetamide (KU-0060648), 2-(4-morpholinyl)-4H-naphtho[1,2-b]pyran-4-one (NU7026), 8-( 4-Dibenzothienyl)-2-(4-morpholinyl)-4H-1-benzopyran-4-one (NU7441, KU-57788), 3-[4-(4-morpholinyl)pyrido[3',2':4,5]furo[3,2-d]pyrimidin-2-yl]-phenol (PI-103), 2-methyl-5-nitro-2-[(6-bromoimidazo[1,2-a]pyridin-3-yl)methylene]-1-methylhydrazide-benzenesulfonic acid, monohydrochloride (PIK-75HCl), 1-cyclopentyl-3-(1H-pyrrolo[2,3-b]pyridin-5-yl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (PP121), SF2523 (CAS N o 1174428-47-7), and analogs thereof. Preferably, the DNA-PKi is (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharma- ceutically acceptable salt thereof.

[0107] According to the second aspect of the invention, and a preferred embodiment of the first aspect of the invention, the DNA-PKi is (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharma- ceutically acceptable salt thereof.

[0108] As used herein, (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol is a DNA-PK inhibitor, also known as M3814 and peposertib. M3814 is described in detail in US Patent Application Publication No. 2016 / 0083401, the entirety of which is incorporated herein by reference. M3814 is designated as compound 136 in Table 4 of US Patent Application Publication No. 2016 / 0083401. M3814 is active in a variety of assays and therapeutic models that demonstrate inhibition of DNA-PK. M3814 is an orally bioavailable, potent and selective ATP-competitive inhibitor of DNA-PK, as demonstrated by crystallographic and enzyme kinetic studies. DNA-PK, together with five other protein factors (Ku70, Ku80, XRCC4, ligase IV, and Artemis), plays a key role in the repair of DSBs via NHEJ. The kinase activity of DNA-PK is essential for proper and timely DNA repair and long-term survival of cancer cells. Without wishing to be bound by any particular theory, it is believed that the primary effect of M3814 is the inhibition of DNA-PK activity and DNA double-strand break (DSB) repair, resulting in altered DNA repair and enhanced antitumor activity of DNA damaging agents.

[0109] Radiation Therapeutic Agents Radiolabeled targeting moieties (also known as radioimmunoconjugates) are designed to target proteins or receptors that are upregulated in disease states and / or specific to diseased cells (e.g., tumor cells) to deliver a radioactive payload to damage and kill the cells of interest. "Radioimmunotherapy" refers to such therapy where the targeting moiety comprises an antibody, usually a monoclonal antibody.

[0110] Radioactive decay of the payload produces alpha, beta, or gamma particles or Auger electrons that can cause direct effects on DNA (such as single- or double-stranded DNA breaks) or indirect effects such as by-stander or crossfire effects.

[0111] Radioimmunoconjugates typically contain a biological targeting moiety (e.g., an antibody or antigen-binding fragment thereof that specifically binds to a molecule expressed on or by a tumor, such as CAIX or PSMA), a chelating moiety or a metal complex of a chelating moiety (e.g., containing a radioisotope), and a linker. Conjugates can be formed by appending a bifunctional chelate to the biological targeting molecule in a manner that minimizes structural changes while maintaining target affinity. Radioimmunoconjugates can be formed by radiolabeling such conjugates.

[0112] Structurally, bifunctional chelates include a chelate, a linker, and a bridging group. When developing new bifunctional chelates, most efforts are focused on the chelating part of the molecule. Several examples of bifunctional chelates have been described with various cyclic and acyclic structures conjugated to targeting moieties.

[0113] As used herein, the term "radioconjugate" refers to any complex that includes a radioisotope or radionuclide (eg, a radioisotope or radionuclide described herein).

[0114] As used herein, the term "radioimmunoconjugate" refers to any immunoconjugate that includes a radioisotope or radionuclide (e.g., a radioisotope or radionuclide described herein). As used herein, the term "immunoconjugate" refers to a conjugate that includes a targeting moiety (such as an antibody or antigen-binding fragment thereof). In some embodiments, the immunoconjugate includes an average of 0.10 or more conjugates per targeting moiety (e.g., an average of 0.2 or more, 0.3 or more, 0.4 or more, 0.5 or more, 0.6 or more, 0.7 or more, 0.8 or more, 0.9 or more, 1 or more, 2 or more, 4 or more, 5 or more, or 8 or more conjugates per targeting moiety).

[0115] The term "radioimmunotherapy" as used herein refers to a method of using a radioimmunoconjugate to produce a therapeutic effect. In some embodiments, radioimmunotherapy can include administration of a radioimmunoconjugate to a subject in need of treatment, where administration of the radioimmunoconjugate produces a therapeutic effect in the subject. In some embodiments, radioimmunotherapy can include administration of a radioimmunoconjugate to a cell, where administration of the radioimmunoconjugate kills the cell. Where radioimmunotherapy involves selective killing of cells, in some embodiments the cells are cancer cells in a subject with cancer.

[0116] As used herein, the term "radionuclide" refers to a compound that is produced by radioactive decay (e.g., 3 H, 14 C. 15 N, 18 F, 35 S, 47 Sc, 55 Co, 60 Cu, 61 Cu, 62 Cu, 64 Cu, 67 Cu, 75 Br, 76 Br, 77 Br, 89 Zr, 86 Y, 87 Y, 90 Y, 97 Ru, 99 Tc, 99 mTc,105 Rh, 109 Pd, 111 ln, 123 I, 124 I, 125 I, 131 I, 149 Pm, 149 Tb, 153 Sm, 166 Ho, 177 Lu, 186 Re, 188 Re, 198 Au, 199 Au, 203 Pb, 211 At, 212 Pb, 212 Bi, 213 Bi, 223 Ra, 225 Ac, 227 Th, 229 Th, 66 Ga, 67 Ga, 68 Ga, 82 Rb, 117 mSn, 201 This refers to an atom that can undergo TI.

[0117] The terms radionuclide, radioisotope, or radioisotope may also be used to describe a radionuclide. As noted above, a radionuclide may be used as a detection agent. In some embodiments, the radionuclide is an alpha-emitting radionuclide.

[0118] antibody As used herein, "antibody" refers to a polypeptide, including immunoglobulins and fragments thereof, whose amino acid sequence specifically binds to a designated antigen or fragment thereof. Antibodies can be of any type (e.g., IgA, IgD, IgE, IgG, or IgM) or subtype (e.g., lgA1, lgA2, lgG1, lgG2, lgG3, or lgG4). One of skill in the art will understand that a characteristic sequence or portion of an antibody can include an amino acid sequence found in one or more regions of an antibody (e.g., variable region, hypervariable region, constant region, heavy chain, light chain, and combinations thereof). Furthermore, one of skill in the art will understand that a characteristic sequence or portion of an antibody can include one or more polypeptide chains and can include sequence elements found in the same polypeptide chain or in different polypeptide chains. Antibodies usually include two identical light polypeptide chains and two identical heavy polypeptide chains linked together by disulfide bonds. The first domain, located at the amino terminus of each chain, is variable in amino acid sequence and provides the antibody binding specificity of each individual antibody. These are known as the variable heavy (VH) and variable light (VL) regions. The other domains of each chain are relatively invariant in amino acid sequence and are known as the constant heavy (CH) and constant light (CL) regions. The light chain usually contains one variable region (VL) and one constant region (CL). IgG heavy chains contain a variable region (VH), a first constant region (CH1), a hinge region, a second constant region (CH2), and a third constant region (CH3). In IgE and IgM antibodies, the heavy chain contains an additional constant region (CH4).

[0119] Methods for generating antibodies are known in the art and / or described in Harlow and Lane (editors) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, (1988).Generally, in such methods, dysfunctional P2X7 receptor or its region (e.g., extracellular region) or its immunogenic fragment or epitope, or cells expressing and presenting it (i.e., immunogen), optionally formulated with any suitable or desired carrier, adjuvant, or pharma- ceutically acceptable excipient, is administered to a non-human animal, such as a mouse, chicken, rat, rabbit, guinea pig, dog, horse, cow, goat, or pig.The immunogen can be administered intranasally, intramuscularly, subcutaneously, intravenously, intradermally, intraperitoneally, or by other known routes.

[0120] The production of polyclonal antibodies can be monitored by sampling the blood of the immunized animal at various times after immunization. One or more booster immunizations may be administered if necessary to achieve the desired antibody titer. The process of boosting and titering is repeated until a suitable titer is achieved. Once a desired level of immunogenicity is obtained, the immunized animal is bled and the serum isolated and stored, and / or the animal is used to generate monoclonal antibodies (mAbs).

[0121] Monoclonal antibodies are one exemplary form of antibody contemplated by the present invention. The term "monoclonal antibody" or "mAb" refers to a homogeneous antibody population capable of binding to the same antigen(s), e.g., the same epitope within an antigen. The term is not intended to be limited with respect to the source of the antibody or the method by which it is made.

[0122] For the production of mAbs, any of a number of well-known techniques can be used, such as the procedures exemplified in US Pat. No. 4,196,265, mentioned above, or Harlow and Lane (1988).

[0123] For example, a suitable animal is immunized with an immunogen under conditions sufficient to stimulate antibody-producing cells.Rodents such as rabbits, mice, and rats are exemplary animals.For example, mice genetically engineered to express human antibodies without expressing mouse antibodies can also be used to generate the antibodies of the present invention (e.g., as described in WO2002 / 066630).

[0124] After immunization, somatic cells with the potential to produce antibodies, specifically B lymphocytes (B cells), are selected for use in mAb generation protocols. These cells can be obtained from biopsies of the spleen, tonsils, or lymph nodes, or from peripheral blood samples. The B cells from the immunized animal are then fused with cells of an immortal myeloma cell, usually from the same species as the animal immunized with the immunogen.

[0125] The hybrids are amplified by culture in selection medium containing drugs that block de novo synthesis of nucleotides in tissue culture medium. Exemplary drugs are aminopterin, methotrexate, and azaserine.

[0126] The amplified hybridomas are subjected to functional selection for antibody specificity and / or titer, for example, by flow cytometry, and / or immunohistochemistry, and / or immunoassays (e.g., radioimmunoassays, enzyme immunoassays, cytotoxicity assays, plaque assays, dot immunoassays, and the like).

[0127] Alternatively, ABL-MYC technology (NeoClone, Madison, Wis. 53713, USA) is used to generate cell lines secreting MAbs (e.g. as described in Largaespada et al, J. Immunol. Methods. 197: 85-95, 1996).

[0128] Antibodies can also be generated or isolated by screening a display library (e.g., a phage display library), e.g., as described in U.S. Patent No. 6,300,064 and / or U.S. Patent No. 5,885,793. For example, the inventors have isolated fully human antibodies from a phage display library.

[0129] An antibody for use in the methods of the invention may be a synthetic antibody, for example, the antibody is a chimeric antibody, a humanized antibody, a human antibody, a synthetic humanized antibody, a primatized antibody, or a deimmunized antibody.

[0130] Antibodies described herein include, for example, monoclonal antibodies, polyclonal antibodies, multispecific antibodies, human antibodies, humanized antibodies, camelid antibodies, chimeric antibodies, single chain Fvs (scFvs), disulfide-linked Fvs (sdFvs), and anti-idiotypic (anti-Id) antibodies, as well as antigen-binding fragments of any of the above. In some embodiments, the antibodies or antigen-binding fragments thereof are humanized. In some embodiments, the antibodies or antigen-binding fragments thereof are chimeric. The antibodies may be of any type (e.g., IgG, IgE, IgM, IgD, IgA, and IgY), class (e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2), or subclass.

[0131] Preferably, the antibody is of a format and size that ensures that the antibody does not undergo substantial renal clearance, but rather undergoes primarily hepatic clearance.

[0132] The term "antigen-binding fragment" of an antibody, as used herein, refers to one or more fragments of an antibody that retain the ability to specifically bind to an antigen. Examples of binding fragments encompassed by the term "antigen-binding fragment" of an antibody include Fab fragments, F(ab')2 fragments, Fd fragments, Fv fragments, scFv fragments, dAb fragments (Ward et al., (1989) Nature 341:544-546), and isolated complementarity determining regions (CDRs). In some embodiments, an "antigen-binding fragment" comprises a heavy chain variable region and a light chain variable region. These antibody fragments can be obtained using conventional techniques known to those of skill in the art, and the fragments can be screened for utility in the same manner as intact antibodies.

[0133] The antibodies or fragments described herein can be produced by any method known in the art for the synthesis of antibodies (see, e.g., Harlow et al., Antibodies: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988); Brinkman et al., 1995, J. Immunol. Methods 182:41-50; WO 92 / 22324; WO 98 / 46645). Chimeric antibodies can be made, for example, using the methods described in Morrison, 1985, Science 229:1202, and humanized antibodies can be made, for example, by the methods described in U.S. Pat. No. 6,180,370.

[0134] Other antibodies described herein are bispecific and multivalent antibodies, e.g., as described in Segal et al., J. Immunol. Methods 248:1-6 (2001), and Tutt et al., J. Immunol. 147: 60 (1991).

[0135] The present invention encompasses antigen binding proteins and / or antibodies described herein comprising an antibody constant region, including an antigen-binding fragment of an antibody fused to Fc.

[0136] The sequences of constant regions useful for producing the proteins of the invention can be obtained from several different sources. In some examples, the constant region of the protein or a portion thereof is derived from a human antibody. The constant region or a portion thereof can be derived from any antibody class, including IgM, IgG, IgD, IgA and IgE, and any antibody isotype, such as IgG1, IgG2, IgG3, and IgG4. In some examples, the constant region is a human isotype IgG4 or stabilized IgG4 constant region.

[0137] In some examples, the Fc region of the constant region has a reduced ability to induce effector function, e.g., compared to a native or wild-type human IgG1 Fc region or human IgG3 Fc region. In some examples, the effector function is antibody-dependent cell-mediated cytotoxicity (ADCC), and / or antibody-dependent cell-mediated phagocytosis (ADCP), and / or complement-dependent cytotoxicity (CDC). Methods for assessing the level of effector function of an Fc region-containing protein are known in the art and / or described herein.

[0138] In one example, the Fc region is an IgG4 Fc region (i.e., from an IgG4 constant region), e.g., a human IgG4 Fc region. Sequences of suitable IgG4 Fc regions will be apparent to those of skill in the art and / or are available in publicly available databases (e.g., available from the National Center for Biotechnology Information).

[0139] In one example, the constant region is a stabilized IgG4 constant region. The term "stabilized IgG4 constant region" is understood to mean an IgG4 constant region that has been modified to reduce the tendency to undergo Fab arm exchange or to form half antibodies or to form half antibodies. "Fab arm exchange" refers to a type of protein modification of human IgG4 in which an IgG4 heavy chain and a binding light chain (half molecule) are exchanged with a heavy chain light chain pair from another IgG4 molecule. Thus, an IgG4 molecule can acquire two different Fab arms that recognize two different antigens (resulting in a bispecific molecule). Fab arm exchange occurs naturally in vivo and can be induced in vitro by purified blood cells or reducing agents such as reduced glutathione. "Half antibodies" are formed when an IgG4 antibody dissociates to form two molecules, each containing a single heavy chain and a single light chain.

[0140] In one example, the stabilized IgG4 constant region contains a proline at position 241 of the hinge region according to the Kabat system (Kabat et al., Sequences of Proteins of Immunological Interest Washington DC United States Department of Health and Human Services, 1987 and / or 1991). This position corresponds to position 228 of the hinge region according to the EU numbering system (Kabat et al., Sequences of Proteins of Immunological Interest Washington DC United States Department of Health and Human Services, 2001, and Edelman et al., Proc. Natl. Acad. USA, 63, 78-85, 1969). In human IgG4, this residue is usually a serine. After the serine is replaced by a proline, the IgG4 hinge region contains the sequence CPPC. In this regard, the skilled artisan will recognize that the "hinge region" is the proline-rich portion of the antibody heavy chain constant region that connects the Fc region and the Fab region, which confers flexibility to the two Fab arms of the antibody. The hinge region contains the cysteine ​​residues involved in the inter-heavy chain disulfide bond. It is generally defined as the stretch from Glu226 to Pro243 of human IgG1 according to the Kabat numbering system. Hinge regions of other IgG isotypes can be aligned with the IgG1 sequence by placing the first and last cysteine ​​residues that form the inter-heavy chain disulfide (SS) bond in the same positions (see, for example, WO 2010 / 080538).

[0141] Another example of a stabilized IgG4 antibody is an antibody in which the arginine at position 409 (according to the EU numbering system) of the heavy chain constant region of human IgG4 has been replaced with lysine, threonine, methionine, or leucine (e.g. as described in WO 2006 / 033386). The Fc region of the constant region may additionally or alternatively comprise a residue selected from the group consisting of alanine, valine, glycine, isoleucine, and leucine at the position corresponding to 405 (according to the EU numbering system). Optionally, the hinge region comprises a proline at position 241 (i.e. the CPPC sequence) (as described above).

[0142] In another example, the Fc region is a region modified to have reduced effector function, i.e., a "non-immunostimulatory Fc region." For example, the Fc region is an IgG1 Fc region that includes substitutions at one or more positions selected from the group consisting of 268, 309, 330, and 331. In another example, the Fc region is an IgG1 Fc region that includes one or more of the following changes: E233P, L234V, L235A, and deletion of G236, and / or one or more of the following changes: A327G, A330S, and P331S (Armour et al., Eur J Immunol. 29:2613-2624, 1999; Shields et al., J Biol Chem. 276(9):6591-604, 2001). Other examples of non-immunostimulatory Fc regions are described, for example, in Dall'Acqua et al., J Immunol. 177: 1129-1138 2006, and / or Hezareh J Virol; 75: 12161-12168, 2001).

[0143] In another example, the Fc region is a chimeric Fc region comprising, for example, at least one CH2 domain from an IgG4 antibody and at least one CH3 domain from an IgG1 antibody, and the Fc region comprises a substitution at one or more amino acid positions selected from the group consisting of 240, 262, 264, 266, 297, 299, 307, 309, 323, 399, 409, and 427 (EU numbering) (e.g., as described in WO 2010 / 085682). Exemplary substitutions include 240F, 262L, 264T, 266F, 297Q, 299A, 299K, 307P, 309K, 309M, 309P, 323F, 399S, and 427F.

[0144] Additional Modifications The present invention also contemplates additional modifications to antibodies or antigen binding proteins comprising the Fc region or constant region.

[0145] For example, the antibody comprises one or more amino acid substitutions that increase the half-life of the protein. For example, the antibody comprises an Fc region that comprises one or more amino acid substitutions that increase the affinity of the Fc region for neonatal Fc region (FcRn). For example, the Fc region has increased affinity for FcRn at lower pH (e.g., about pH 6.0) to promote Fc / FcRn binding in endosomes. In one example, the Fc region has increased affinity for FcRn at about pH 6 compared to its affinity at about pH 7.4, promoting re-release of Fc into blood after cellular recycling. These amino acid substitutions are useful for extending the half-life of the protein by reducing clearance from blood.

[0146] Exemplary amino acid substitutions include T250Q and / or M428L or T252A, T254S and T266F or M252Y, S254T and T256E or H433K and N434F according to the EU numbering system. Additional or alternative amino acid substitutions are described, for example, in U.S. Patent Application Publication No. 20070135620 or U.S. Patent Application Publication No. 7,083,784.

[0147] Antibodies for binding to CAIX As used herein, CAIX refers to the transmembrane protein carbonic anhydrase IX (CAIX), a member of a large family of carbonic anhydrases that share the ability to catalyze the reversible hydration of carbon dioxide to carbonic acid, resulting in a decrease in pH. Upregulation of CAIX gene expression occurs in response to hypoxia via direct transcriptional activation by hypoxia-inducible factor-1 alpha (HIF-1a) and is believed to be involved in sensing and maintaining an acidic environment in hypoxic cells, particularly those within hypoxic regions of tumors.

[0148] The terms "carbonic anhydrase IX" and "CAIX," "CA9," "MN," and "G250" may be used interchangeably.

[0149] CAIX is highly expressed in various tumor types, has relatively low expression in normal tissues, plays an important role in tumor progression, acidification, and metastasis, and is located on the extracellular surface of the cell membrane, allowing for efficient targeting by antibodies or small molecule inhibitors.

[0150] A variety of molecules are known for binding to CAIX, including radiolabeled small molecules, antibodies, and antibody fragments for use in immunohistochemistry or immunoimaging techniques.

[0151] Anti-CAIX antibodies, variants and fragments thereof are described, for example, in EP 637336, WO 93 / 18152, WO 95 / 34650, WO 00 / 24913, WO 02 / 063010, WO 04 / 025302, WO 05 / 037083, WO 2011 / 139375, WO 2014 / 096163, WO 2019 / 122025 and their foreign counterparts. In addition, WO 02 / 062972 describes the hybridoma cell line DSM ACC 2526, which produces the monoclonal antibody G250. Monoclonal antibody G250 recognizes an antigen that is preferably expressed on the membrane of renal cell carcinoma cells (RCC), but not on normal proximal tubule epithelium.

[0152] In another preferred embodiment, the antibody and / or antibody fragment thereof is selected from the group consisting of polyclonal antibodies, monoclonal antibodies, antigen-binding fragments thereof such as F(ab')2, Fab', sFv, dsFv, and chimeric, humanized, and fully human variants thereof. According to a further preferred embodiment, the anti-CAIX antibody or epitope-binding fragment thereof binds to the amino acid sequence LSTAFARV and / or ALGPGREYRAL.

[0153] According to a further particularly preferred embodiment, the CAIX targeting compound is the antibody cG250 (e.g. as described in EP 0637336) and / or epitope-binding fragments thereof. Preferably, the CAIX targeting molecule is a chimeric or humanized G250 antibody and / or fragments thereof. Antibodies for use in the present invention can be made by any suitable method known in the art, including but not limited to the methods described in International Application Nos. PCT / EP02 / 01282 and PCT / EP02 / 01283, which are incorporated herein by reference.

[0154] A particularly preferred antibody is cG250, preferably direntuximab (INN), also referred to herein as GmAb. Another particularly preferred embodiment is the monoclonal antibody G250 produced by the hybridoma cell line DSM ACC 2526. The antibody cG250 is an IgG1 kappa light chain chimeric version of the originally murine monoclonal antibody mG250.

[0155] In a particularly preferred embodiment, the antibodies for binding to CAIX are those described in WO 2021 / 000017, the contents of which are incorporated herein by reference (e.g., GmAb and the radiolabeled conjugate form DOTA-GmAb). Radioimmunoconjugates for binding to CAIX are also described in WO 2021 / 000017, and have a reduced serum half-life (i.e., increased rate of serum clearance), as do radioimmunoconjugates for binding to CAIX.

[0156] In a particularly preferred embodiment, the antibody for binding to CAIX is FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 and FR1a-CDR1a-FR2a-CDR2a-FR3a-CDR3a-FR4a where FR1, FR2, FR3, and FR4 are framework regions, CDR1, CDR2, and CDR3 are complementarity determining regions, FR1a, FR2a, FR3a, and FR4a are framework regions, CDR1a, CDR2a, and CDR3a are complementarity determining regions, The sequence of any of said complementarity determining regions has an amino acid sequence as set out in Table 2 below. Preferably, the framework regions have an amino acid sequence also as set out in Table 2 below, including amino acid mutations at specific residues that can be determined by aligning the various framework regions from each antibody. The present invention also includes the cases where CDR1, CDR2 and CDR3 are sequences from a VH and CDR1a, CDR2a and CDR3a are sequences from a VL, or where CDR1, CDR2 and CDR3 are sequences from a VL and CDR1a, CDR2a and CDR3a are sequences from a VH.

[0157] Preferably, the antibody is in the format FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4-linker-FR1a-CDR1a-FR2a-CDR2a-FR3a-CDR3a-FR4a, or FR1a-CDR1a-FR2a-CDR2a-FR3a-CDR3a-FR4a-FR1-CDR1-FR2-CDR2-FR3a-CDR3a-FR4a-FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4.

[0158] As defined herein, the linker may be a chemical entity, one or more amino acids, or a disulfide bond formed between two cysteine ​​residues. In a preferred embodiment, the linker consists of one or more amino acid residues.

[0159] Antibodies for specifically binding to CAIX preferably comprise an antigen-binding site that consists essentially of or consists of the amino acid sequence of SEQ ID NO:52, SEQ ID NO:68, SEQ ID NO:84, SEQ ID NO:100, or SEQ ID NO:116 (in N-terminal to C-terminal or C-terminal to N-terminal order).

[0160] In other embodiments, an antibody that specifically binds to CAIX is (i) a VH comprising a complementarity determining region (CDR) 1 comprising a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 49, SEQ ID NO: 65, SEQ ID NO: 81, SEQ ID NO: 97, or SEQ ID NO: 113; a CDR2 comprising a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 50, SEQ ID NO: 66, SEQ ID NO: 82, SEQ ID NO: 98, or SEQ ID NO: 114; and a CDR3 comprising a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 51, SEQ ID NO: 67, SEQ ID NO: 83, SEQ ID NO: 99, or SEQ ID NO: 115; (ii) a VH comprising a sequence that is about 95% or more, about 96% or more, about 97% or more, about 98% or more, or about 99% or more identical to the sequences set forth in SEQ ID NO:52, SEQ ID NO:68, SEQ ID NO:84, SEQ ID NO:100, and SEQ ID NO:116; (iii) CDR1 comprising a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 129, SEQ ID NO: 145, SEQ ID NO: 161, SEQ ID NO: 177, SEQ ID NO: 193, or SEQ ID NO: 209; a CDR2 comprising a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 131, SEQ ID NO: 147, SEQ ID NO: 163, SEQ ID NO: 179, SEQ ID NO: 195, or SEQ ID NO: 211; (iv) a VL comprising a sequence that is about 95% or more identical to a sequence set forth in SEQ ID NO: 132, SEQ ID NO: 148, SEQ ID NO: 164, SEQ ID NO: 180, SEQ ID NO: 196, or SEQ ID NO: 212; (v) a VH comprising a CDR1 comprising the sequence set forth in SEQ ID NO: 49, SEQ ID NO: 65, SEQ ID NO: 81, SEQ ID NO: 97, or SEQ ID NO: 113, a CDR2 comprising the sequence set forth in SEQ ID NO: 50, SEQ ID NO: 66, SEQ ID NO: 82, SEQ ID NO: 98, or SEQ ID NO: 114, and a CDR3 comprising the sequence set forth in SEQ ID NO: 51, SEQ ID NO: 67, SEQ ID NO: 83, SEQ ID NO: 99, or SEQ ID NO: 115; (vi) VH comprising the sequences set forth in SEQ ID NO: 52, SEQ ID NO: 68, SEQ ID NO: 84, SEQ ID NO: 100, and SEQ ID NO: 116; (vii) a VL comprising a CDR1 comprising the sequence set forth in SEQ ID NO: 129, SEQ ID NO: 145, SEQ ID NO: 161, SEQ ID NO: 177, SEQ ID NO: 193, or SEQ ID NO: 209, a CDR2 comprising the sequence set forth in SEQ ID NO: 130, SEQ ID NO: 146, SEQ ID NO: 162, SEQ ID NO: 178, SEQ ID NO: 194, or SEQ ID NO: 210, and a CDR3 comprising the sequence set forth in SEQ ID NO: 131, SEQ ID NO: 147, SEQ ID NO: 163, SEQ ID NO: 179, SEQ ID NO: 195, or SEQ ID NO: 211; (viii) a VL comprising the sequence set forth in SEQ ID NO: 132, SEQ ID NO: 148, SEQ ID NO: 164, SEQ ID NO: 180, SEQ ID NO: 196, or SEQ ID NO: 212; (ix) a VH comprising a CDR1 comprising the sequence shown in SEQ ID NO: 49, SEQ ID NO: 65, SEQ ID NO: 81, SEQ ID NO: 97, or SEQ ID NO: 113, a CDR2 comprising the sequence shown in SEQ ID NO: 50, SEQ ID NO: 66, SEQ ID NO: 82, SEQ ID NO: 98, or SEQ ID NO: 114, and a CDR3 comprising the sequence shown in SEQ ID NO: 51, SEQ ID NO: 67, SEQ ID NO: 83, SEQ ID NO: 99, or SEQ ID NO: 115, and a VL comprising a CDR1 comprising the sequence shown in SEQ ID NO: 129, SEQ ID NO: 145, SEQ ID NO: 161, SEQ ID NO: 177, SEQ ID NO: 193, or SEQ ID NO: 209, a CDR2 comprising the sequence shown in SEQ ID NO: 130, SEQ ID NO: 146, SEQ ID NO: 162, SEQ ID NO: 178, SEQ ID NO: 194, or SEQ ID NO: 210, and a CDR3 comprising the sequence shown in SEQ ID NO: 131, SEQ ID NO: 147, SEQ ID NO: 163, SEQ ID NO: 179, SEQ ID NO: 195, or SEQ ID NO: 211, or (x) a VH comprising the sequence shown in SEQ ID NO: 52, SEQ ID NO: 68, SEQ ID NO: 84, SEQ ID NO: 100, or SEQ ID NO: 116, and a VL comprising the sequence shown in SEQ ID NO: 132, SEQ ID NO: 148, SEQ ID NO: 164, SEQ ID NO: 180, SEQ ID NO: 196, or SEQ ID NO: 212 Contains at least one of the following:

[0161] Preferably, the heavy chain constant region contains amino acid substitutions at both His310 and His435. The antibody may also contain amino acid substitutions at residues equivalent to Ser228 and Leu235 of the constant heavy chain region.

[0162] Preferably, the antibody comprises a heavy chain constant region comprising the sequence shown in any one of SEQ ID NO:225 to SEQ ID NO:228, preferably the sequence shown in SEQ ID NO:226.

[0163] In yet another embodiment, the antibody preferably comprises a heavy chain comprising the sequence shown in any one of SEQ ID NO:230 to SEQ ID NO:233, preferably the sequence shown in SEQ ID NO:231.

[0164] In some embodiments, the antibody comprises a light chain constant region comprising the amino acid sequence set forth in SEQ ID NO: 229. Preferably, the antibody comprises a light chain comprising the amino acid sequence set forth in SEQ ID NO:234.

[0165] In a particular preferred embodiment, the antibody comprises the sequence set forth in SEQ ID NO:231 and the sequence set forth in SEQ ID NO:234.

[0166] Antibodies for binding to PSMA As used herein, PSMA refers to "prostate-specific membrane antigen."

[0167] Preferably, the antibody for binding to PSMA interacts with (e.g., binds to) the extracellular domain of PSMA, e.g., the extracellular domain of human PSMA located approximately at amino acids 44-750 of human PSMA (the amino acid residues correspond to the human PSMA sequence disclosed in U.S. Pat. No. 5,538,866). In some embodiments, the antibody binds to a PSMA dimer, e.g., the agent binds to a portion of PSMA exposed in both the PSMA dimer and the PSMA monomer, or the agent binds to a portion of PSMA exposed in the PSMA dimer but not in the PSMA monomer. Preferably, the interaction (e.g., binding) occurs with high affinity and specificity. Preferably, the PSMA binding agent treats, e.g., eliminates or kills, a cell, e.g., a PSMA-expressing cell (e.g., a cancerous cell or a vascular endothelial cell). The mechanism by which the PSMA binding agent treats, e.g., eliminates or kills, a cell is not critical to the practice of the invention. In some embodiments, the PSMA-binding agent can bind and internalize PSMA expressed in the cell and / or in vascular endothelial cells adjacent to the cell. In those embodiments, the binding agent can be used to target a second moiety (e.g., a cytotoxic agent) to the cell. In other embodiments, the PSMA-binding agent, upon binding to the extracellular domain of PSMA, can mediate host-mediated killing (e.g., complementation or ADCC-mediated killing) of the cell and / or vascular cells adjacent thereto. The cell can be killed directly by a PSMA-binding agent that directly binds to the cell (e.g., for cancerous cells) or to vascular endothelial cells adjacent thereto. Alternatively, the PSMA-binding agent can treat, e.g., kill or eliminate, or otherwise change the properties of the vascular endothelial cells to which it binds, resulting in reduced blood flow to the cells in its vicinity, thereby killing or eliminating the cells in its vicinity.

[0168] An "anti-PSMA antibody" is an antibody that interacts with (e.g., binds to) PSMA (preferably human PSMA) protein. The antibody can be any PSMA-specific antibody (e.g., a monospecific antibody, or a recombinant or modified antibody), including antigen-binding fragments thereof.

[0169] Anti-PSMA antibodies and fragments thereof are known, including antibodies that bind to PSMA, preferably human PSMA, with high affinity and specificity. In some embodiments, the antibody is an antibody having one or more complementarity determining regions (CDRs) from the J591, J415, J533, or E99 antibodies, or from an antibody that competes with one of these antibodies or has an overlapping epitope with one of these antibodies. The antibody J591 is described in Liu et al., Cancer Res 1997; 57: 3629-34. In any embodiment of the invention, the anti-PSMA binding antibody or antigen-binding fragment thereof may have a light chain variable region that includes one or more complementarity determining regions (CDRs) from a monoclonal antibody selected from the group consisting of J591, J415, J533, and E99, or from an antibody that competes with or has an epitope that overlaps with one of these antibodies, and / or a heavy chain variable region that includes one or more CDRs from a monoclonal antibody selected from the group consisting of J591, J415, J533, and E99, or from an antibody that competes with or has an epitope that overlaps with one of these antibodies. In some embodiments, the antibody or antigen-binding portion thereof includes all six CDRs from murine J591, or all six CDRs from murine J415.In other embodiments, the antibody is an antibody having one or more complementarity determining regions (CDRs) from a 4A3, 7F12, 8A11, 8C12, 16F9 026, or PSMA4.40 antibody, or from an antibody that competes with one of these antibodies or has an overlapping epitope with one of these antibodies, e.g., a light chain variable region having one or more complementarity determining regions (CDRs) from a monoclonal antibody selected from the group consisting of 4A3, 7F12, 8A11, 8C12, 16F9 026, and PSMA 4.40, or from an antibody that competes with one of these antibodies or has an overlapping epitope with one of these antibodies, and / or a light chain variable region having one or more complementarity determining regions (CDRs) from a monoclonal antibody selected from the group consisting of 4A3, 7F12, 8A11, 8C12, 16F9 026, and PSMA 4.40, and / or an antibody that competes with or has an overlapping epitope with one of these antibodies. In some embodiments, the antibody or antigen-binding portion thereof comprises all six CDRs from one of the foregoing antibodies.

[0170] Other antibodies for binding to PSMA that are contemplated for use in accordance with the present invention include those disclosed in U.S. Patent No. 20190022205 (the "10B3" antibody), the contents of which, the specific antibody sequences of which are incorporated herein by reference.

[0171] In some embodiments, the anti-PSMA monospecific antibody is a monoclonal antibody, a chimeric antibody, a CDR-grafted antibody, a humanized antibody (e.g., a humanized mouse antibody), a deimmunized antibody (e.g., a deimmunized mouse antibody), or a human antibody, or an antigen-binding fragment thereof. The anti-PSMA antibody (e.g., a recombinant antibody or a modified antibody) can be full-length (e.g., IgG (e.g., IgG1, IgG2, IgG3, IgG4), IgM, IgA (e.g., IgAl, IgA2), IgD and IgE, but preferably IgG) or can comprise only an antigen-binding fragment (e.g., a Fab, F(ab')2 or scFv fragment, or one or more CDRs). The antibody or antigen-binding fragment thereof can comprise two heavy chain immunoglobulins and two light chain immunoglobulins, or can be a single chain antibody. The antibody may optionally comprise a constant region selected from a kappa constant region gene, a lambda constant region gene, an alpha constant region gene, a gamma constant region gene, a delta constant region gene, an epsilon constant region gene, or a mu constant region gene. Preferred anti-PSMA antibodies comprise a heavy chain constant region and / or a light chain constant region substantially derived from a human antibody (e.g., a human IgG1 constant region) or a portion thereof. In some embodiments, the anti-PSMA antibody is a human antibody.

[0172] The antibody (or fragment thereof) may be a murine antibody or a human antibody. Examples of murine monoclonal antibodies that may be used include E99, J415, J533, and J591 antibodies, which are produced by hybridoma cell lines with ATCC accession numbers HB-12101, HB-12109, HB-12127, and HB-12126, respectively. Methods and compositions using antibodies or antigen-binding fragments thereof that bind to overlapping epitopes or competitively inhibit the binding of the anti-PSMA antibodies disclosed herein to PSMA (e.g., antibodies that bind to overlapping epitopes or competitively inhibit the binding of one or more of monoclonal antibodies E99, J415, J533, J591, 4A3, 7F12, 8A11, 8C12, 16F9 026, or PSMA 4.40 to PSMA) are also within the scope of the present invention. Any combination of anti-PSMA antibodies can be used, for example, two or more antibodies that bind to different regions of PSMA, for example, antibodies that bind to two different epitopes on the extracellular domain of PSMA.

[0173] In some embodiments, the binding agent is an anti-PSMA antibody that binds to all or a portion of the epitope of an antibody described herein, e.g., the J591, E99, J415, J533, 4A3, 7F12, 8A11, 8C12, 16F9 026, and PSMA 4.40 antibodies. The anti-PSMA antibody may inhibit (e.g., competitively inhibit) the binding of an antibody described herein, e.g., the J591, E99, J415, J533, 4A3, 7F12, 8A11, 8C12, 16F9 026, and PSMA 4.40 antibodies, to human PSMA. The anti-PSMA antibody may bind to an epitope (e.g., a conformational or linear epitope) which, when bound, prevents binding of the antibodies described herein, J591, E99, J415, J533, 4A3, 7F12, 8A11, 8C12, 16F9 026, and PSMA 4.40 antibodies. The epitope may be in close spatial proximity or functionally related, for example, an epitope that overlaps or is adjacent in linear sequence or conformationally to that recognized by the J591, E99, J415, J533, 4A3, 7F12, 8A11, 8C12, 16F9 026, or PSMA 4.40 antibodies.

[0174] In certain embodiments, the anti-PSMA antibody binds to an epitope located wholly or partially within a region of about amino acids 120-500 (e.g., 130-450, 134-437, or 153-347) of human PSMA. Typically, the epitope includes at least one glycosylation site, e.g., at least one N-linked glycosylation site (e.g., an N-linked glycosylation site located at about amino acids 190-200, preferably at about amino acid 195, of human PSMA).

[0175] In other embodiments, the antibody (or antigen-binding fragment thereof) is a recombinant or modified anti-PSMA antibody, e.g., selected from a chimeric antibody, a CDR-grafted antibody, a humanized antibody, a deimmunized antibody, or an in vitro generated antibody (or antigen-binding fragment thereof). As discussed herein, the modified antibody can be a CDR-grafted antibody, a humanized antibody, a deimmunized antibody, or, more generally, an antibody having CDRs derived from a non-human antibody (e.g., mouse J591 antibody, mouse J415 antibody, mouse J533 antibody, or mouse E99 antibody) and a framework selected as being less immunogenic in humans (e.g., less antigenic than the mouse framework naturally occurring in the mouse CDRs). In some embodiments, the modified antibody is a deimmunized anti-PSMA antibody, such as a deimmunized form of E99, J415, J533, or J591 (e.g., a deimmunized form of the antibody produced by the hybridoma cell lines having ATCC Accession Nos. HB-12101, HB-12109, HB-12127, and HB-12126, respectively).

[0176] Typically, the antibody is a deimmunized form of J591 or J415 (referred to herein as "deJ591" or "deJ415," respectively). Most preferably, the antibody is a deimmunized form of J591. The antibody can be a human antibody, e.g., a human antibody made in a non-human animal (e.g., a mouse).

[0177] The antibody or antigen-binding fragment thereof has one or more, two or more, preferably three CDRs consisting of the heavy chain variable region of murine J591 (as defined in SEQ ID NOs: 1, 2, and 3 and shown in Figure 1A of US Patent Publication No. 20060088539, which is incorporated herein by reference), and the light chain variable region of murine J591 (see SEQ ID NOs: 4, 5, and 6 shown in Figure 1B of US Patent Publication No. 20060088539, which is incorporated herein by reference). The antibody or antigen-binding fragment thereof may have the heavy chain variable and light chains of the J591 antibody or any modified form thereof as described in Figures 1A and 1B of US Patent Publication No. 20060088539. The antibody or antigen-binding fragment thereof may have the heavy chain variable and light chain of the deimmunized J591 antibody or any modified form thereof, as depicted in Figures 2A and 2B of US Patent Publication No. 20060088539.

[0178] As used herein, ANT4044 and ANT4044-A2 refer to humanized and affinity matured humanized versions of the J591 antibody, respectively, the sequences of which are shown in Table 1 herein.

[0179] In a particularly preferred embodiment, the antibodies for binding to PSMA are those described in WO 2021 / 000017, the contents of which are incorporated herein by reference. Radioimmunoconjugates for binding to PSMA are also described in WO 2021 / 000017.

[0180] In a preferred embodiment, the antibody specifically binds to prostate specific membrane antigen (PSMA), FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 and FR1a-CDR1a-FR2a-CDR2a-FR3a-CDR3a-FR4a where FR1, FR2, FR3, and FR4 are framework regions, CDR1, CDR2, and CDR3 are complementarity determining regions, FR1a, FR2a, FR3a, and FR4a are framework regions, CDR1a, CDR2a, and CDR3a are complementarity determining regions, The sequence of any of the complementarity determining regions has the amino acid sequence set out in Table 1 below. Preferably, the framework regions have the amino acid sequence also set out in Table 1 below, including amino acid mutations at specific residues that can be determined by aligning the various framework regions from each antibody. The present invention also includes the cases where CDR1, CDR2 and CDR3 are sequences from VH and CDR1a, CDR2a and CDR3a are sequences from VL, or where CDR1, CDR2 and CDR3 are sequences from VL and CDR1a, CDR2a and CDR3a are sequences from VH.

[0181] Preferably, the antibody is in the format FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4-linker-FR1a-CDR1a-FR2a-CDR2a-FR3a-CDR3a-FR4a, or FR1a-CDR1a-FR2a-CDR2a-FR3a-CDR3a-FR4a-FR1-CDR1-FR2-CDR2-FR3a-CDR3a-FR4a-FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4.

[0182] As defined herein, the linker may be a chemical entity, one or more amino acids, or a disulfide bond formed between two cysteine ​​residues. In a preferred embodiment, the linker consists of one or more amino acid residues.

[0183] More preferably, the antibody for binding PSMA comprises an antigen-binding site consisting essentially of or consisting of the amino acid sequence of SEQ ID NO:4 or SEQ ID NO:20, and / or SEQ ID NO:36 (in N-terminal to C-terminal order or C-terminal to N-terminal order).

[0184] In other embodiments, an antibody that specifically binds PSMA is (i) a VH comprising a complementarity determining region (CDR) 1 comprising a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 1, SEQ ID NO: 17, or SEQ ID NO: 244; a CDR2 comprising a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 2 or SEQ ID NO: 18; and a CDR3 comprising a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 3 or SEQ ID NO: 19; (ii) a VH comprising a sequence that is about 95% or more, about 96% or more, about 97% or more, about 98% or more, or about 99% or more identical to the sequence set forth in SEQ ID NO: 4, SEQ ID NO: 20, or SEQ ID NO: 245; (iii) a VL comprising a CDR1 having a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 33, a CDR2 having a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 34, and a CDR3 having a sequence that is about 80% or more, about 85% or more, about 90% or more, about 92% or more, about 95% or more, about 97% or more, or about 99% or more identical to the sequence shown in SEQ ID NO: 35; (iv) a VL comprising a sequence that is about 95% or more identical to the sequence set forth in SEQ ID NO: 36 or SEQ ID NO: 246; (v) a VH comprising a CDR1 comprising the sequence shown in SEQ ID NO:1, SEQ ID NO:17, or SEQ ID NO:244, a CDR2 comprising the sequence shown in SEQ ID NO:2 or SEQ ID NO:18, and a CDR3 comprising the sequence shown in SEQ ID NO:3 or SEQ ID NO:19; (vi) a VH comprising a sequence as set forth in SEQ ID NO: 4, SEQ ID NO: 20, or SEQ ID NO: 245; (vii) VL comprising a CDR1 comprising the sequence shown in SEQ ID NO: 33, a CDR2 comprising the sequence shown in SEQ ID NO: 34, and a CDR3 comprising the sequence shown in SEQ ID NO: 45; (viii) a VL comprising the sequence set forth in SEQ ID NO: 36 or SEQ ID NO: 246; (ix) a VH comprising a CDR1 comprising the sequence shown in SEQ ID NO: 1 or SEQ ID NO: 17, a CDR2 comprising the sequence shown in SEQ ID NO: 2 or SEQ ID NO: 18, and a CDR3 comprising the sequence shown in SEQ ID NO: 3 or SEQ ID NO: 19, and a VL comprising a CDR1 comprising the sequence shown in SEQ ID NO: 33, a CDR2 comprising the sequence shown in SEQ ID NO: 34, and a CDR3 comprising the sequence shown in SEQ ID NO: 35; or (x) a VH comprising a sequence as set forth in SEQ ID NO: 4, SEQ ID NO: 20, or SEQ ID NO: 245, and a VL comprising a sequence as set forth in SEQ ID NO: 36 or SEQ ID NO: 246; Contains at least one of the following:

[0185] In a preferred embodiment, the antibody or antigen-binding fragment thereof comprises heavy chain CDRs having the amino acid sequences set forth in SEQ ID NO:244, SEQ ID NO:18, and SEQ ID NO:19, and light chain CDRs set forth in SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35.

[0186] In a further preferred embodiment, the antibody or antigen-binding fragment thereof comprises heavy chain CDRs having the amino acid sequences set forth in SEQ ID NO:1, SEQ ID NO:18, and SEQ ID NO:19, and light chain CDRs set forth in SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35.

[0187] In certain embodiments, the heavy chain constant region comprises amino acid substitutions at both His310 and His435. The antibody may also comprise amino acid substitutions at residues equivalent to Ser228 and Leu235 of the constant heavy chain region.

[0188] In any embodiment, the antibody comprises a heavy chain constant region comprising the amino acid sequence set forth in any one of SEQ ID NO:235 to SEQ ID NO:237, and preferably, the heavy chain constant region comprises the sequence set forth in SEQ ID NO:236.

[0189] In yet another embodiment, the heavy chain of the antibody comprises the sequence shown in any one of SEQ ID NO: 239 to SEQ ID NO: 242, preferably the sequence shown in SEQ ID NO: 239, and more preferably the sequence shown in SEQ ID NO: 245.

[0190] Furthermore, in a preferred embodiment, the light chain constant region of the antibody comprises the sequence set forth in SEQ ID NO: 238. More preferably, the antibody comprises a light chain comprising the amino acid sequence set forth in SEQ ID NO: 243. Most preferably, the antibody comprises a light chain comprising the amino acid sequence set forth in SEQ ID NO: 246.

[0191] In a particularly preferred embodiment, the antibody comprises the amino acid sequence set forth in SEQ ID NO:239 and the sequence set forth in SEQ ID NO:243.

[0192] Antibodies for binding to other tumor-associated or tumor-specific antigens It will be appreciated that according to any aspect of the invention, the radioimmunoconjugate comprises an antibody or fragment thereof for binding to an antigen expressed by the cancer in need of treatment. It is well within the purview of one of skill in the art to identify suitable antibodies for use in the treatment of any given cancer.

[0193] In the context of the present invention, "tumor antigen" or "hyperproliferative disorder antigen" or "hyperproliferative disorder associated antigen" refers to an antigen common to a particular hyperproliferative disorder, such as cancer. The antigens discussed herein are included as examples only. The list is not intended to be exhaustive, and other examples will be readily apparent to one of skill in the art.

[0194] Tumor antigens are proteins produced by tumor cells that elicit an immune response, particularly a T cell-mediated immune response. The choice of antigen binding moiety of the invention will depend on the particular type of cancer being treated, and tumor antigens are well known in the art and include, for example, glioma-associated antigen, carcinoembryonic antigen (CEA), β-human chorionic gonadotropin, alpha fetoprotein (AFP), lectin-reactive AFP, thyroglobulin, RAGE-1, MN-CA IX, human telomerase reverse transcriptase, RU1, RU2 (AS), intestinal carboxylesterase, mut hsp70-2, M-CSF, prostase, prostate specific antigen (PSA), PAP, NY-ESO-1, LAGE-1a, p53, prostein, PSMA, Her2 / neu, survivin and telomerase, prostate carcinoma tumor antigen-1 (PCTA-1), MAGE, ELF2M, neutrophil elastase, ephrin B2, CD22, insulin growth factor (IGF)-I, IGF-II, IGF-I receptor, and mesothelin.

[0195] In some embodiments, the tumor antigen comprises one or more antigenic cancer epitopes associated with malignant tumors. Malignant tumors express several proteins that can serve as target antigens for immune attack. These molecules include, but are not limited to, tissue-specific antigens, such as MART-1, tyrosinase and GP 100 in melanoma, and prostatic acid phosphatase (PAP) and prostate-specific antigen (PSA) in prostate cancer. Other target molecules belong to the group of transformation-associated molecules, such as the oncogene HER-2 / Neu / ErbB-2. Yet another group of target antigens are carcinoembryonic antigens, such as carcinoembryonic antigen (CEA). In B-cell lymphomas, tumor-specific idiotypic immunoglobulins constitute truly tumor-specific immunoglobulin antigens unique to individual tumors. B-cell differentiation antigens, such as CD19, CD20 and CD37, are other candidates for target antigens in B-cell lymphomas.

[0196] The type of tumor antigen referred to in the present invention can also be a tumor-specific antigen (TSA) or a tumor-associated antigen (TAA). A TSA is unique to tumor cells and is not present in other cells in the body. A TAA-associated antigen is not unique to tumor cells, but instead is also expressed on normal cells under conditions that cannot induce a state of immune tolerance to the antigen. The expression of an antigen on a tumor can occur under conditions that allow the immune system to respond to the antigen. A TAA can be an antigen that is expressed on normal cells during fetal development, when the immune system is immature and unable to respond, or it can be an antigen that is usually present at very low levels on normal cells, but is expressed at much higher levels on tumor cells.

[0197] Non-limiting examples of TSA or TAA antigens include differentiation antigens such as MART-1 / MelanA (MART-1), gp100 (Pmel 17), tyrosinase, TRP-1, TRP-2, and tumor-specific multilineage antigens (e.g., MAGE-1, MAGE-3, BAGE, GAGE-1, GAGE-2, p15); overexpressed embryonic antigens such as CEA; overexpressed oncogenes and mutated tumor suppressor genes such as p53, Ras, HER-2 / neu; unique tumor antigens resulting from chromosomal translocations such as BCR-ABL, E2A-PRL, H4-RET, 1GH-IGK, MYL-RAR; and viral antigens such as the Epstein-Barr virus antigen EBVA, and human papillomavirus (HPV) antigens E6 and E7. Other large protein antigens include TSP-180, MAGE-4, MAGE-5, MAGE-6, RAGE, NY-ESO, p185erbB2, p180erbB-3, c-met, nm-23H1, PSA, TAG-72, CA19-9, CA72-4, CAM17.1, NuMa, K-ras, beta-catenin, CDK4, Mum-1, p15, p16, 43-9F, 5T4, 791Tgp72, alpha-fetoprotem, beta-HCG, BCA225, and BTA. A, CA125, CA15-3\CA27.29\BCAA, CA195, CA242, CA-50, CAM43, CD68\P1, CO-029, FGF-5, G250, Ga733\EpCAM, HTgp-175, M344, MA-50, MG7-Ag, MOV18, NB / 70K, NY-CO-1, RCAS1, SDCCAG16, TA-90\Mac-2 binding protein\cyclophilin C-related protein, TAAL6, TAG72, TLP, and TPS.

[0198] In other embodiments, antibodies for use in radioimmunoconjugates may be modified to decrease the serum half-life of the antibody, which can be accomplished by modifying the antibody to have reduced FcRn binding affinity, as described in WO 2021 / 000017.

[0199] In certain preferred embodiments, the one or more amino acid substitutions may be at one or more of IgG residues His310, His433, His435, His436, or Ile253. Preferably, the amino acid substitution comprises a substitution at position His310 or His435 in the heavy chain constant region. More preferably, the amino acid substitution that reduces the affinity of the antibody for FcRn is at both His310 and His435.

[0200] In certain embodiments, the modified antibodies retain the ability to bind to one or more Fc-gamma receptors and thus, in certain embodiments, the modified antibodies retain the ability to stimulate an effector response (including ADCC).

[0201] In alternative embodiments, the one or more amino acid modifications that reduce affinity for the FcRn receptor also reduce affinity for an Fc gamma receptor. The modified antibody may further comprise one or more amino acid substitutions compared to a wild-type antibody of class IgG, which amino acid substitutions further reduce the affinity of the antibody for one or more Fc gamma receptors.

[0202] In other embodiments, the modified antibody further comprises one or more amino acid substitutions compared to a wild-type antibody of class IgG, which amino acid substitutions increase the stability of the CH1-CH2 hinge region of the modified antibody compared to a wild-type antibody of class IgG.

[0203] The modified antibody of class IgG having reduced FcRn binding affinity compared to an unmodified antibody of class IgG can be any antibody useful for targeting diagnostic or therapeutic agents to a biological site. The antibody can be of any IgG class, such as IgG1 (human or mouse), IgG2, IgG4, mouse IgG2a, etc.

[0204] Linkers and Crosslinks In any aspect of the invention, the antibody or fragment thereof is conjugated to a radionuclide. The radionuclide may be directly or indirectly conjugated to the antibody, for example, by halogenation of an amino acid residue. Preferably, the radionuclide agent is indirectly conjugated to the antibody via a linker or chelator moiety.

[0205] In another example, the antibody is conjugated to a bifunctional linker (e.g., bromoacetyl, thiol, succinimide ester, TFP ester, maleimide) or using any amine or thiol modification chemistry known in the art.

[0206] The term "chelate" as used herein refers to an organic compound or portion thereof that is capable of binding to a central metal or radioactive metal atom at two or more positions.

[0207] The term "conjugate" as used herein refers to a molecule that comprises a chelating group or a metal complex thereof, a linker group, and, optionally, a therapeutic moiety, a targeting moiety, or a bridging group.

[0208] Examples of suitable chelating moieties include DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10 tetraacetic acid), DOTMA (1R,4R,7R,10R)-α,α',α'',α'''-tetramethyl-1.4.7.10-tetraazacyclododecane-1,4,7,10 tetraacetic acid, DOTAM (1,4,7,10-tetrakis(carbamoylmethyl)-1,4,7,10-tetraazacyclododecane), DOTPA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrapropionic acid), DOTAM-acetate (2-(4,7,10-tris(2-amino-2-oxoethyl)-1,4,7,10-tetraazacyclododecane- 1-yl)acetic acid), DOTA-GA anhydride (2,2',2''-(10-(2,6-dioxotetrahydro-2H-pyran-3-yl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid, DOTP (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetra(methylenephosphonic acid)), DOTMP (1,4,6,10-tetraazacyclodecane-1,4,7,10-tetramethylenephosphonic acid, DOTA-4AMP (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(acetamido-methylenephosphonic acid), CB-TE2A (1,4,8,11-tetraazabicyclo[6.6.2]hexadecane-4,11-diacetic acid), NOTA (1,4,7-triazacyclononane-1,4,7-triacetic acid), NOTP (1,4,7-triazacyclononane-1,4,7-tri(methylenephosphonic acid), TETPA (1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetrapropionic acid), TETA (1,4,8,11-tetraazacyclotetradecane- 1,4,8,11-tetraacetic acid), HEHA (1,4,7,10,13,16-hexaazacyclohexadecane-1,4,7,10,13,16-hexaacetic acid), PEPA (1,4,7,10,13-pentaazacyclopentadecane-N,N',N'',N''',N''''-pentaacetic acid), H4Octapa (N,N'-bis(6-carboxy-2-pyridylmethyl)-ethylenediamine-N, N'-diacetic acid), H2dedpa (1,2-[[6-(carboxy)-pyridin-2-yl]-methylamino]ethane), H6phospa (N,N'-(methylenephosphonate)-N,N'-[6-(methoxycarbonyl)pyridin-2-yl]-methyl-1,2-diaminoethane), TTHA (triethylenetetramine-N,N,N',N'',N''',N'''-hexaacetic acid), DO2P (tetraazacyclododecane dimethane phosphonic acid), HP-DO3A (hydroxypropyltetraazacyclododecane triacetic acid), EDTA (ethylenediaminetetraacetic acid), deferoxamine, DTPA (diethylenetriaminepentaacetic acid), DTPA-BMA (diethylenetriaminepentaacetic acid bismethylamide), HOPO (octadentate hydroxypyridinone), or porphyrin.

[0209] In some embodiments, the radioimmunoconjugate comprises a metal complex of a chelating moiety. For example, chelating groups can be used in metal chelate combinations of metals such as manganese, iron, and gadolinium with isotopes such as any of the radioisotopes and radionuclides discussed herein (e.g., isotopes in the general energy range of 60 keV to 4,000 keV).

[0210] In some embodiments, the radioimmunoconjugate comprises a crosslinking group. A crosslinking group is a reactive group capable of covalently linking two or more molecules. A crosslinking group can be used to attach a linker and a chelating moiety to a therapeutic or targeting moiety. A crosslinking group can also be used to attach a linker and a chelating moiety to a target in vivo. In some embodiments, the crosslinking group is an amino-reactive crosslinking group, a methionine-reactive crosslinking group, or a thiol-reactive crosslinking group, or a sortase-mediated coupling.

[0211] In some embodiments, the amino-reactive or thiol-reactive crosslinking group comprises an activated ester, such as a hydroxysuccinimide ester, a 2,3,5,6-tetrafluorophenol ester, a 4-nitrophenol ester, or an imidate, an anhydride, a thiol, a disulfide, a maleimide, an azide, an alkyne, a strained alkyne, a strained alkene, a halogen, a sulfonate, a haloacetyl, an amine, a hydrazide, a diazirine, a phosphine, a tetrazine, an isothiocyanate, or an oxaziridine. In some embodiments, the sortase recognition sequence may comprise a terminal glycine-glycine-glycine (GGG) and / or a LPTXG amino acid sequence, where X is any amino acid. One of skill in the art will appreciate that the use of crosslinking groups is not limited to the particular constructs disclosed herein, but may include other known crosslinking groups.

[0212] Other Treatments In an optional embodiment, the method of the invention comprises the additional step (iii) of administering an antiproliferative agent, a radiosensitizer, or an immunomodulator or immunomodulatory agent.

[0213] As used herein, "antiproliferative agents" refers to any anti-cancer chemotherapy agent. The term "antiproliferative agents" may be used interchangeably with the terms "antineoplastic agents" or "cytotoxic agents". Antiproliferative agents may be alkylating agents, platinum agents, antimetabolites, topoisomerase inhibitors, anthracycline antibiotics, mitotic inhibitors, aromatase inhibitors, thymidylate synthase inhibitors, DNA antagonists, farnesyltransferase inhibitors, pump inhibitors, histone acetyltransferase inhibitors, metalloproteinase inhibitors, ribonucleoside reductase agents, TNFα agonists / antagonists, endothelin A receptor anatoagonists, kinase inhibitors.

[0214] Such agents include organoplatinum derivatives, naphthoquinone and benzoquinone derivatives, chrysophanic acid and its anthroquinone derivatives.

[0215] As used herein, "immune regulator", "immunomodulatory agent", or "immunomodulation", used interchangeably herein, refers to any immunomodulatory agent, such as those selected from the group consisting of interferon, oncaphage, nivolumab, abatacept, pembrolizumab, ipilimumab, and atezolizumab.

[0216] As used herein, "radiosensitizer" refers to any agent that increases the sensitivity of cancer cells to radiation therapy.Radiosensitizers can include, but are not limited to, 5-fluorouracil, platinum analogs (e.g., cisplatin, carboplatin, oxaliplatin), gemcitabine, EGFR antagonists (e.g., cetuximab, gefitinib), farnesyltransferase inhibitors, COX-2 inhibitors, bFGF antagonists, and VEGF antagonists.

[0217] As used herein, the term "chemotherapeutic agent" refers to a chemical compound that is effective in inhibiting tumor growth. Examples of chemotherapeutic agents include alkylating agents such as thiotepa and cyclophosphamide; alkylsulfonates such as busulfan, improsulfan, and piposulfan; aziridines such as benzodopa, carboquone, metoledopa, and uredopa; ethylenimines and methylamelanamines such as altretamine, triethylenemelamine, triethylenephosphoramide, triethylenethiophosphaarnide, and trimethylmelamine; acetogenins (particularly bullatacin and bullatacinone); carmptothecin (including the synthetic analog topotecan); bryostatin; kallistatin; CC-1065 (including its adozelesin synthetic analog, carzelesin synthetic analog, and bizelesin synthetic analog); cryptophycins (particularly cryptophycin 1 and cryptophycin 2). 8); dolastatins; duocarmycins (including the synthetic analogs KW-2189 and CBI-TMI); eleutherobin; pancratistatin; sarcodictin; spongistatins; nitrogen mustards such as chlorambucil, chromafazine, chlorophosphamide, estralnustine, ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, nobembitine, phenesterine, prednimustine, trophosfamide, and uracil mustard; nitrosoureas such as carmustine, chlorozotocin, fotemustine, lomustine, nimustine, and ranimustine; antibiotics such as enediyne antibiotics (e.g., calicheamicin); dynemycins, including dynemycin A; esperamicin;and neocarzinostatin chromophore and related chromoprotein enediyne antibiotic chromophores), aclacinomycin, actinomycin, ausramycin, azaserine, bleomycin, cactinomycin, carabicin, canninomycin, carzinophilin, chromomycin, dactinomycin, daunorubicin, detrevicin, 6-diazo-5-oxo-L-norleucine, doxorubicin (morpholino-doxorubicin, cyanomorpholino-doxorubicin, 2-pyrrolidine-doxorubicin, and deoxydoxo rubicin, epirubicin, esorubicin, idanrubicin, marcelomycin, mitomycin, mycophenolic acid, nogalamycin, olivomycin, peplomycin, pofilomycin, puromycin, queramycin, rodorubicin, streptomugrin, streptozocin, tubercidin, ubenimex, zinostatin, zorubicin; antimetabolites such as methotrexate and 5-fluorouracil (5-FU); folic acid analogues such as denopterin, methotrexate, pteropterin, trimetrexate; fludarabine, 6-mer Purine analogues such as captopurine, thiamiprine, and thioguanine; pyrimidine analogues such as ancitabine, azacitidine, 6-azauridine, carmoful, cytarabine, dideoxyuridine, doxifluridine, enocitabine, floxuridine, and 5-FU; androgens such as calsterone, dromostanolone propionate, epithiostanol, mepitiostane, and testolactone; antiadrenergics such as aminoglutethimide, mitotane, and trilostane; folic acid supplements such as floric acid; aceglatone; aldophospharnide glycosides; aminolevulinic acid; nicotinic acid;amsacrine;bestrabucil;bisantrene;edatraxate;defofamine;demecolcine;diaziquone;elfornitine;elliptinium acetate;epothilone;etoglucide;gallium nitrate;hydroxyurea;lentinan;lonidamine;maytansinoids such as maytansine and ansamitocin;mitoguazone;mitoxantrone;mopidamol;nitracrine;pentostatin;phenamet;pirarubicin;podophyllic acid;2-ethylhydrazide;procarbazine;PSK(registered trademark);razoxane;rhizoxin;sizofiran;Spirogenanium; tenuazonic acid; triazicon; 2,2',2"-trichlorotriethylamine; trichothecenes (especially T-2 toxin, veracrine A, roridin A, and anguidin); urethane; vindesine; dacarbazine; mannomustine; mitobromtol; mitolactol; pipobroman; gacytosine; arabinoside ("Ara-C"); cyclophosphamide; thiotepa; taxoids such as paclitaxel (TAXOL®, described in Bristol-Myers Squibb Oncology, Princeton, N.) and doxetaxel (TAXOTERE®, Rhone-Poulenc Rorer, Antony, France); chlorambucil; gemcitabine; 6-thioguanine; mercaptopurine; methotrexate; platinum analogues such as cisplatin and carboplatin; vinblastine; platinum; etoposide (VP-16); ifosfamide; mitomycin C; mitoxantrone; vincristine; vinorelbine; navelbine; novantrone; teniposide; daunomycin; aminopterin; xeloda; ibandronate; CPT-11; topoisomerase inhibitor RFS2000; difluoromethylornithine (DMFO); retinoic acid; capecitabine; and pharmaceutically acceptable salts, acids, or derivatives of any of these. Also included within this definition are antihormonal agents which act to regulate or inhibit hormone action on tumors, e.g., antiestrogens including tamoxifen, raloxifene, aromatase-inhibiting 4(5)-imidazoles, 4-hydroxytamoxifen, trioxifene, keoxifene, LY117018, onapristone, and toremifene (Fareston), and antiandrogens such as flutamide, nilutamide, bicalutamide, leuprolide, and goserelin; and pharmaceutically acceptable salts, acids, or derivatives of any of these;

[0218] In an optional embodiment, the method includes the additional step of administering an immunomodulatory agent, which may be selected from immune checkpoint modulators, preferably inhibitors of PD-1, PD-L1, and CTLA-4, or any other immune checkpoint inhibitors described herein.

[0219] Optionally, the immune checkpoint inhibitor is an inhibitor of PD-1 selected from pembrolizumab, nivolumab, cemiplimab, spartalizumab, canrelizumab, sintilimab, tislelizumab, toripalimab, dostarimab, INCMGA00012, AMP-224, and AMP-514.

[0220] Optionally, the immune checkpoint inhibitor is an inhibitor of PD-L1 selected from atezolizumab, avelumab, durvalumab, KN035, CK-301, AUNP12, CA-170, and BMS-986189.

[0221] The immune checkpoint inhibitor can be an inhibitor of CTLA-4 selected from ipilimumab and tremelimumab.

[0222] Indication to be treated The present invention relates to methods of treating diseases or conditions characterized by abnormal cellular function. Typically, such diseases and conditions include cancer, but it will be understood that other proliferative conditions characterized by abnormal cellular proliferation may also be included.

[0223] As used herein, the term "cancer" refers to a malignant growth or tumor resulting from the uncontrolled division of cells. The term "cancer" includes primary and metastatic tumors and generally refers to any disease caused by the proliferation of malignant neoplastic cells, such as tumors, neoplasms, carcinomas, sarcomas, leukemias, and lymphomas. "Solid tumor cancers" are cancers that involve an abnormal mass of tissue, e.g., sarcomas, carcinomas, and lymphomas. "Blood cancers" or "liquid cancers," used interchangeably herein, are cancers that are present in bodily fluids, e.g., lymphomas and leukemias.

[0224] In any of the embodiments herein, the cancer may be a metastatic cancer.

[0225] Examples of cancers that can be treated according to the methods of the present invention include pre-neoplastic and neoplastic diseases. Broad examples include breast tumors, colorectal tumors, adenocarcinomas, mesothelioma, bladder tumors, prostate tumors, germ cell tumors, hepatoma / bile duct tumors, carcinomas, neuroendocrine tumors, pituitary neoplasms, small round cell tumors, squamous cell carcinomas, melanomas, atypical fibroxanthomas, seminomas, non-seminomas, interstitial Leydig cell tumors, Sertoli cell tumors, skin tumors, kidney tumors, testicular tumors, brain tumors, ovarian tumors, stomach tumors, oral tumors, bladder tumors, bone tumors, cervical tumors, esophageal tumors, laryngeal tumors, liver tumors, lung tumors, vaginal tumors, and Wilms' tumors.

[0226] Furthermore, cancer may specifically include, but is not limited to, the following histological types: neoplasm, malignant; carcinoma; carcinoma, undifferentiated; giant cell and spindle cell carcinoma; small cell carcinoma; papillary carcinoma; squamous cell carcinoma; lymphoepithelial carcinoma; basal cell carcinoma; pilomatrimatous carcinoma; transitional cell carcinoma; papillary transitional cell carcinoma; adenocarcinoma; gastrinoma, malignant; cholangiocarcinoma; hepatocellular carcinoma; mixed hepatocellular and cholangiocarcinoma; trabecular adenocarcinoma; adenoid cystic carcinoma; adenocarcinoma in adenomatous polyps; adenocarcinoma, familial polyposis coli; solid tumors; carcinoid tumor, malignant; branching alveolar adenocarcinoma; papillary adenocarcinoma; chromophobe carcinoma; eosinophilic carcinoma; eosinophilic adenocarcinoma; basophilic Carcinoma;Clear cell adenocarcinoma;Granular cell carcinoma;Follicle adenocarcinoma;Papillary and follicular adenocarcinoma;Non-encapsulated sclerosing carcinoma;Adrenal cortical carcinoma;Endometrial carcinoma;Cutaneous adnexal carcinoma;Apocrine gland carcinoma;Sebaceous gland carcinoma;Earwax gland carcinoma;Mucous epidermoid carcinoma;Cystadenocarcinoma;Papillary cystadenocarcinoma;Papillary serous cystadenocarcinoma;Mucinous cystadenocarcinoma;Mucinous adenocarcinoma;Signet ring cell carcinoma;Invasive ductal carcinoma;Medullary carcinoma;Lobular carcinoma;Inflammatory carcinoma;Paget's disease, acinar cell carcinoma of the breast;Adenosquamous carcinoma;Adenocarcinoma with squamous metaplasia;Malignant thymoma;Malignant ovarian stromal tumor;Malignant meningioma;Malignant granulosa cell tumor;And malignant blastoma;Sertoli cell carcinoma;Malignant Leydig cell tumor;Malignant lipocytoma;Malignant paraganglioma;malignant extramammary paraganglioma;pheochromocytoma;glomus sarcoma;malignant melanoma;amelanotic melanoma;superficial spreading melanoma;malignant melanoma in giant pigmented nevus;epithelioid cell melanoma;malignant blue nevus;sarcoma;fibrosarcoma;malignant fibrous histiocytoma;myxosarcoma;liposarcoma;leiomyosarcoma;rhabdomyosarcoma;embryonal rhabdomyosarcoma;alveolar rhabdomyosarcoma;stromal sarcoma;malignant mixed tumor;Müllerian mixed tumor;nephroblastoma;hepatoblastoma;carcinosarcoma;malignant mesenchymoma;malignant Brenner tumor;malignant phyllodes tumor;synovial sarcoma;malignant mesothelioma;dysgerminoma;embryonal carcinoma;malignant teratoma;malignant ovarian stroma;choriocarcinoma;malignant mesonephroma;hemangiosarcoma;malignant hemangioendothelioma;Kaposi's sarcoma;malignant hemangiopericytoma;lymphangiosarcoma;osteosarcoma;paracortical osteosarcoma;chondrosarcoma;malignant chondroblastoma;mesenchymal chondrosarcoma;giant cell tumor of bone;Ewing's sarcoma;malignant odontogenic tumor;ameloblastic odontoma;malignant ameloblastoma;ameloblastic fibrosarcoma;malignant pinealoma;chordoma;malignant glioma;ependymoma;astrocytoma;protoplasmic astrocytoma;fibrous astrocytoma;astroblastoma;glioma;oligodendroglioma;oligodendroglioma;primitive neuroectodermal tumor;cerebellar sarcoma;gangliongioblastoma;neuroblastoma;retinoblastoma;Olfactory neurogenic tumor; malignant meningioma; neurofibrosarcoma; malignant neurilemmoma; malignant granular cell tumor; malignant lymphoma; Hodgkin's disease; Hodgkin's lymphoma; lateral granuloma; malignant lymphoma, small lymphocytic lymphoma; diffuse large cell malignant lymphoma; follicular malignant lymphoma; mycosis fungoides; other specific non-Hodgkin's lymphoma; malignant histiocytosis; multiple myeloma; mast cell sarcoma; immunoproliferative small intestinal disease; leukemia; lymphocytic leukemia; plasma cell leukemia; erythroleukemia; lymphosarcoma cell leukemia; myeloid leukemia; basophilic leukemia; eosinophilic leukemia; monocytic leukemia; mast cell leukemia; megakaryoblastic leukemia; myeloid sarcoma; and hairy cell leukemia.;

[0227] In certain embodiments, the cancer is renal cancer. As used herein, the terms "kidney cancer", "renal cancer", or "renal cell carcinoma" refer to cancer originating from the kidney. As used herein, the terms "renal cell carcinoma" or "renal cell carcinoma" (RCC) refer to cancer originating from the lining of the proximal convoluted tubule. More specifically, RCC encompasses several relatively common histological subtypes: clear cell renal cell carcinoma, papillary (pigmented), chromophobe, collecting duct carcinoma, and medullary carcinoma. Clear cell renal cell carcinoma (ccRCC) is the most common subtype of RCC. In certain embodiments, the cancer is metastatic renal cell carcinoma.

[0228] In a preferred embodiment, the cancer is a cancer characterized by expression of CAIX. The cancer characterized by expression of CAIX may be, but should not be construed as being limited to, clear cell renal cell carcinoma, head and neck cancer, cervical cancer, pancreatic cancer, non-small cell lung cancer, gastroesophageal cancer, and hepatocellular carcinoma. In a particularly preferred embodiment, the cancer characterized by expression of CAIX is clear cell renal cell carcinoma, optionally metastatic renal cell carcinoma. In such an embodiment, the radiotherapeutic agent for use in the treatment of cancer comprises an antibody for binding to CAIX, preferably an antibody as described herein, and most preferably the antibody is conjugated to a beta-emitting radioligand.

[0229] In certain embodiments, the cancer is prostate cancer.As used herein, the term "prostate cancer" refers to cancer originating from the prostate.In certain embodiments, the cancer is metastatic prostate cancer.In certain embodiments, the cancer is metastatic castration-resistant prostate cancer (mCRPC).

[0230] In certain embodiments, the cancer is characterized by expression of PSMA and may be selected from prostate cancer, bladder cancer, testicular cancer, neuroendocrine cancer, renal cell carcinoma, and breast cancer. In particularly preferred embodiments, the cancer characterized by expression of PSMA is prostate cancer, optionally metastatic prostate cancer, e.g., metastatic castration-resistant prostate cancer. In such embodiments, the radiotherapeutic agent for use in treating cancer comprises an antibody for binding to PSMA, preferably an antibody as described herein, and most preferably, the antibody is conjugated to a beta-emitting radioligand.

[0231] In any embodiment, the cancer to be treated by the combination is most preferably a cancer whose cancer cells express or differentially express the catalytic subunits of DNA-PK, the respective DNA-PKAcs, and whose cancer cells exhibit DNA-PK, the respective DNA-PKAcs activity.

[0232] Other diseases and conditions include various inflammatory conditions. Examples include proliferative components. Specific examples include acne, angina, arthritis, aspiration pneumonia, disease, empyema, gastroenteritis, inflammation, intestinal flu, knee, necrotizing enterocolitis, pelvic inflammatory disease, pharyngitis, PID, pleurisy, sore throat, redness, redness, sore throat, stomach flu and urinary tract infection, chronic inflammatory demyelinating polyneuropathy, chronic inflammatory demyelinating polyradiculoneuropathy, chronic inflammatory demyelinating polyneuropathy or chronic inflammatory demyelinating polyradiculoneuropathy.

[0233] Treatment and Administration Methods In some disclosed methods, a treatment (e.g., including a therapeutic agent) is administered to a subject. In some embodiments, the subject is a mammal, e.g., a human.

[0234] In some embodiments, the subject has cancer or is at risk of developing cancer. For example, the subject may have been diagnosed with cancer. The cancer may be primary cancer or metastatic cancer. The subject may have cancer at any stage, e.g., stage I, stage II, stage III, or stage IV, with or without lymph node invasion, with or without metastasis. The provided compositions may prevent or reduce further growth of the cancer and / or ameliorate the cancer (e.g., prevent or reduce metastasis). In some embodiments, the subject does not have cancer but has been determined to be at risk of developing cancer due to the presence of one or more risk factors, such as, for example, environmental exposure, the presence of one or more genetic mutations or variants, family history, etc. In some embodiments, the subject has not been diagnosed with cancer.

[0235] In some embodiments, the cancer is a solid tumor. The solid tumor cancer may be breast cancer, non-small cell lung cancer, small cell lung cancer, pancreatic cancer, head and neck cancer, prostate cancer, colorectal cancer, sarcoma, adrenocortical carcinoma, neuroendocrine carcinoma, Ewing's sarcoma, multiple myeloma, or acute myeloid leukemia.

[0236] In a preferred embodiment, the solid tumor cancer may be a solid tumor cancer characterized by expression of CAIX, such as clear cell renal cell carcinoma, head and neck cancer, cervical cancer, pancreatic cancer, non-small cell lung cancer, gastroesophageal cancer, and hepatocellular carcinoma. In a particularly preferred embodiment, the cancer characterized by expression of CAIX is clear cell renal cell carcinoma.

[0237] In a more preferred embodiment, the cancer may be a cancer characterized by expression of PSMA, such as prostate cancer, bladder cancer, testicular cancer, neuroendocrine cancer, renal cell carcinoma, and breast cancer. In a particular embodiment, the cancer is metastatic prostate cancer. In a particular embodiment, the cancer is metastatic castration-resistant prostate cancer (mCRPC).

[0238] In some embodiments, the cancer is a non-solid (e.g., liquid (e.g., blood)) cancer.

[0239] As used herein, the term "effective amount" of an agent (e.g., any of the conjugates described above) is an amount sufficient to effect beneficial or desired results, such as clinical results, and thus an "effective amount" will vary depending on the context in which it is applied.

[0240] As used herein, the terms "administered in combination," "co-administration," or "co-administered" mean that two or more agents are administered to a subject at the same time, or within an interval during which the effects of each agent on the patient may overlap. Thus, two or more agents that are administered in combination need not be administered together. In some embodiments, they are administered within 90 days (e.g., within 80 days, within 70 days, within 60 days, within 50 days, within 40 days, within 30 days, within 20 days, within 10 days, within 5 days, within 4 days, within 3 days, within 2 days, or within 1 day), within 28 days (e.g., within 14 days, within 7 days, within 6 days, within 5 days, within 4 days, within 3 days, within 2 days, or within 1 day), within 24 hours (e.g., within 12 hours, within 6 hours, within 5 hours, within 4 hours, within 3 hours, within 2 hours, or within 1 hour, or within about 60 minutes, within about 30 minutes, within about 15 minutes, within about 10 minutes, within about 5 minutes, or within about 1 minute) of each other. In some embodiments, the administration of the agents is spaced close enough together that a combined effect is achieved.

[0241] In a preferred embodiment, the radioimmunotherapy agent is administered prior to administration of the DNA-PKi. Preferably, the DNA-PKi is administered within 28 days (e.g., 14, 7, 6, 5, 4, 3, 2, or 1 day) of the radioimmunotherapy agent, most preferably within 1 day, 2 days, 3 days, 4 days, or 5 days of the radioimmunotherapy agent, particularly within 1 day of the radioimmunotherapy agent.

[0242] As used herein, "administering" an agent to a subject includes contacting cells of the subject with the agent.

[0243] The present disclosure provides combination therapy, in which the amount of each therapeutic agent may be therapeutically effective by itself or may not be therapeutically effective by itself. For example, a method is provided that includes administering a first therapy and a second therapy in an amount that is effective in total to treat or improve a disorder (e.g., cancer). In some embodiments, at least one of the first therapy and the second therapy is administered to the subject at a low effective dose. In some embodiments, both the first therapy and the second therapy are administered at a low effective dose.

[0244] The term "low effective dose" when used in combination with a drug (e.g., a therapeutic agent) refers to a dosage of the drug that is therapeutically effective in the combination therapy of the present invention that is lower than the dose that has been determined to be therapeutically effective when the drug is used as a monotherapy in reference experiments or by other therapeutic guidance.

[0245] In some embodiments, the first therapy comprises a radioimmunoconjugate and the second therapy comprises a DNA-PK inhibitor (DNA-PKi).

[0246] In some embodiments, the first treatment comprises a DNA-PKi and the second treatment comprises a radioimmunoconjugate.

[0247] In some embodiments, the therapeutic combinations disclosed herein are administered to a subject in a manner (e.g., dosage and timing) sufficient to cure or at least partially halt the symptoms of the disorder and its complications. In the context of a single treatment ("monotherapy"), an amount sufficient to achieve this purpose is defined as a "therapeutically effective amount", which is an amount of compound sufficient to substantially ameliorate at least one symptom associated with a disease or condition. A "therapeutically effective amount" typically varies depending on the therapeutic agent. For known therapeutic agents, the relevant therapeutically effective amount is known to or can be readily determined by one of ordinary skill in the art.

[0248] For example, in the treatment of cancer, an agent or compound that reduces, prevents, delays, inhibits, or stops any symptoms of the disease or condition would be therapeutically effective. A therapeutically effective amount of an agent or compound is one that does not require a cure of the disease or condition, but rather provides treatment for the disease or condition such that the onset of the disease or condition is delayed, hindered, or prevented, or the symptoms of the disease or condition are ameliorated, or the duration of the disease or condition is altered, or, for example, the severity is reduced or recovery is accelerated in an individual. For example, a treatment may be therapeutically effective if it regresses the cancer or stops or slows the growth of the cancer.

[0249] Effective dosing regimens for these uses (e.g., the amount of each therapeutic agent, the relative timing of the therapeutic agents, etc.) may vary depending on the severity of the disease or condition, as well as the weight and general condition of the subject. For example, one of skill in the art can determine the therapeutically effective amount of a particular composition comprising a therapeutic agent to be administered to a mammal (e.g., a human), taking into account individual differences in the mammal's age, weight, and condition.

[0250] Those skilled in the art can also empirically determine therapeutically effective and / or optimal amounts. Accordingly, those skilled in the art can also determine low effective doses.

[0251] Single or multiple administrations of the composition (e.g., a pharmaceutical composition comprising a therapeutic agent) can be carried out with the dose level and pattern selected by the treating physician. The dose and administration schedule can be determined and adjusted based on the severity of the subject's disease or condition, and the course of treatment can be monitored according to methods typically practiced by clinicians or as described herein.

[0252] In some embodiments, a composition (such as a composition comprising a radioimmunoconjugate) is administered for radiation therapy planning or diagnostic purposes. When administered for radiation therapy planning or diagnostic purposes, the composition may be administered to a subject in an amount effective to determine a diagnostically and / or therapeutically effective dose.

[0253] In some embodiments, a first dose of the disclosed radioimmunoconjugate or a composition thereof (e.g., a pharmaceutical composition) is administered in an amount effective for a radiation treatment regimen, followed by a combination therapy comprising a conjugate disclosed herein and another therapeutic agent.

[0254] In the disclosed combination therapy methods, the first and second therapies may be administered to a subject sequentially or simultaneously. For example, a first composition comprising a first therapeutic agent and a second composition comprising a second therapeutic agent may be administered to a subject sequentially or simultaneously. Alternatively or additionally, a composition comprising a combination of a first therapeutic agent and a second therapeutic agent may be administered to a subject.

[0255] In some embodiments, the radioimmunoconjugate is administered in a single dose. In some embodiments, the radioimmunoconjugate is administered multiple times. When the radioimmunoconjugate is administered multiple times, the dose of each administration may be the same or different.

[0256] In some embodiments, the DNA-PKi is administered in a single dose. In some embodiments, the DNA-PKi is administered more than once, for example, two or more times, three or more times, etc. In some embodiments, the DNA-PKi is administered multiple times according to a regular or semi-regular schedule, for example, approximately once every two weeks, once a week, twice a week, three times a week, or more than three times a week. When the DNA-PKi is administered multiple times, the dose of each administration may be the same or different. For example, the DNA-PKi may be administered at an initial dose, and then the subsequent doses of DNA-PKi may be higher or lower than the initial dose.

[0257] In some embodiments, the first dose of DNA-PKi is administered simultaneously with the first dose of radioimmunoconjugate. In some embodiments, the first dose of DNA-PKi is administered prior to the first dose of radioimmunoconjugate. In some embodiments, the first dose of DNA-PKi is administered after the first dose of radioimmunoconjugate. In some embodiments, a subsequent dose of DNA-PKi is administered.

[0258] In some embodiments, the radioimmunoconjugate (or composition thereof) and the DNA-PKis (or composition thereof) are administered within 28 days (e.g., within 14 days, within 7 days, within 6 days, within 5 days, within 4 days, within 3 days, within 2 days, or within 1 day) of each other. In various embodiments, the DNA-PKi is administered simultaneously with the radioimmunoconjugate.

[0259] In some embodiments, the radioimmunoconjugate (or composition thereof) and the DNA-PKis (or composition thereof) are administered within 90 days (e.g., within 80 days, within 70 days, within 60 days, within 50 days, within 40 days, within 30 days, within 20 days, within 10 days, within 5 days, within 4 days, within 3 days, within 2 days, or within 1 day) of each other. Most preferably, the DNA-PKi is administered within 1 day, within 2 days, within 3 days, within 4 days, or within 5 days, particularly within at least 1 day, of the radioimmunoconjugate.

[0260] In various embodiments, DNA-PKi is administered multiple times after the first administration of radioimmunoconjugate.For example, DNA-PKi can be administered daily throughout the course of treatment.For example, DNA-PKi can be administered daily for 7 days or more, 10 days or more, 14 days or more, 21 days, 28 days or more, or more throughout the duration of treatment regimen.

[0261] For example, according to a particularly preferred treatment protocol, the patient is administered a radioimmunotherapy agent on day 1 of the treatment plan, as described herein. On day 2, treatment with DNA-PKi, preferably M3814, is initiated, where DNA-PKi is administered daily during the course of the treatment regimen. The treatment regimen can include administration of an initial dose of radioimmunotherapy followed by one or two or more subsequent administrations of radioimmunotherapy, for example, for about 7 days or more, about 14 days or more, about 21 days or more, about 28 days or more, about 35 days or more, about 42 days or more, or more.

[0262] In various embodiments, the treatment may include two or more treatment cycles with a washout period ("treatment break") between treatment cycles to allow blood counts to return to normal or near-normal values. In such embodiments, the treatment may include two or more cycles of treatment, optionally a third or fourth cycle, where each treatment cycle includes administration of a radiotherapy agent on the first day of the cycle, followed by administration of a DNA-PKi on subsequent days of the treatment cycle (e.g., starting on day 2, day 3, or day 4, preferably day 2), including administration of the DNA-PKi until at least day 7, at least day 14, or at least day 21 of the treatment cycle. The period between the end of the first treatment cycle and the start of the second treatment cycle (i.e., the period during which the DNA-PKi and radiotherapy agent are not administered) may be 7 days or more, 14 days or more, 21 days or more, 28 days or more, 35 days or more, 42 days or more, or more. The second treatment cycle may be substantially the same or identical to the first treatment cycle (e.g., comprising administration of a radiotherapeutic agent on day 1 of the second treatment cycle followed by administration of a DNA-PKi on a later day of the cycle, preferably starting on day 2 and continuing for 7 or more days, 14 or more days, 21 or more days, or more).

[0263] The second treatment cycle may differ from the first treatment cycle insofar as the second treatment cycle may include administration of DNA-PKi for a shorter period of time after administration of the radiotherapeutic agent or may include administration of DNA-PKi for a longer period of time after administration of the radiotherapeutic agent.

[0264] In any aspect of the invention, the molecular targeted radiotherapeutic agent is administered at a dosage level below the level required for a monotherapy response. This indicates a synergistic effect between the molecular targeted radiotherapeutic agent and the DNA-PKi. Preferably, the molecular targeted radiotherapeutic agent is administered at a dosage of more than 10%, preferably more than 20% less radioactivity compared to a monotherapy response (i.e., a treatment involving administration of only the molecular targeted radiotherapeutic agent), preferably 20-50% less radioactivity compared to a monotherapy response. In a preferred embodiment, the dosage of the radioimmunotherapeutic agent is about 50% or more of the dosage required for a therapeutic effect when administered as a monotherapy.

[0265] For example, radioimmunotherapy agents have a dose of approximately 500MBq / m 2 ~About 3000MBq / m 2 , preferably about 800MBq / m 2 ~About 2000MBq / m 2 , about 1000MBq / m 2 ~About 1800MBq / m 2 , more preferably about 1000MBq / m 2 ~About 1500MBq / m 2 , and most preferably about 1100MBq / m 2 ~About 1500MBq / m 2 In particular, the radiation can be administered to a subject in the form of a beta-emitting radionuclide ( 177 Lutetium or 188 It is supplied in the form of rhenium.

[0266] In another example, the radioimmunotherapy agent is about 10 mCi / m 2 ~about 80mCi / m 2 , about 20mCi / m 2 ~about 60mCi / m 2 , about 25mCi / m 2 ~about 70mCi / m 2 , about 20mCi / m 2 ~about 50mCi / m 2 , preferably about 25 mCi / m 2 ~about 40mCi / m 2 In particular, the radiation can be administered in the form of a beta-emitting radionuclide (177 Lutetium or 188 It is supplied in the form of rhenium.

[0267] Those skilled in the art will recognize the standard 1.7m 2 The above radiation doses for adult individuals are MBq / m 2 For example, in some instances, a radioimmunotherapy agent may be converted to a 1887 MBq (standard 1.7 m 2 1110MBq / m in an adult individual 2 dose), or 2516MBq (standard 1.7m 2 1480MBq / m in an adult individual 2 dose), or 3145MBq (standard 1.7m 2 1850MBq / m in an adult individual 2 The dose will be administered at 100 mg / kg / day.

[0268] In such an embodiment, the DNA-PKi may be administered daily, if desired, at a dose of 0.02 mg to 100 mg / kg body weight, preferably 0.02 mg to 50 mg / kg body weight. The daily dose may specifically be 0.02 mg to 100 mg / kg body weight. The DNA-PKi may be administered once a day at a dose of 50 mg to 400 mg, more preferably 100 mg to 200 mg. Alternatively, the DNA-PKi may be administered twice a day (bid) at a dose of 150 mg to 400 mg.

[0269] DNA-PKi may be administered at a dose of 0.01 mg to 1 g per administration unit, preferably 1 mg to 700 mg per unit, particularly preferably 5 mg to 200 mg per unit, for example, 50 mg or 100 mg per unit.

[0270] In a particularly preferred embodiment, the DNA-PKi is M3814 and the dosage regimen is about 150 mg to 600 mg, preferably 200 mg to 500 mg, more preferably 300 mg to 400 mg, which is administered twice daily.

[0271] Alternatively or most preferably, in addition to the dose of the molecularly targeted radiotherapy agent, the DNA-PKi is administered at a dose level below the maximum tolerated dose level, for example, at a dose of 90% or less, 85% or less, 80% or less, 75% or less, 60% or less, 65% or less, 60% or less, or 55% or less of the maximum tolerated dose level, and / or at a dose of 10% or more, or 20% or more, 30% or more, 40% or more, or 50% or more of the maximum tolerated dose level of the combination.

[0272] In particularly preferred embodiments, the DNA-PKi is M3814 and the dosing regimen is within one of the following ranges: 25 mg to 600 mg, 50 mg to 600 mg, 100 mg to 600 mg, 150 mg to 600 mg, 175 mg to 500 mg, 200 mg to 500 mg, 300 mg to 400 mg, 50 mg to 300 mg, 75 mg to 275 mg, 100 mg to 250 mg, or a combination thereof. In particularly preferred embodiments, the aforementioned doses are administered once daily, but may advantageously be administered twice daily (bid). M3814 may be administered, for example, at doses of 75 mg, 100 mg, 125 mg, 150 mg, 175 mg, 200 mg, 250 mg, 275 mg or 300 mg, 350 mg, or 400 mg, preferably once daily, but also suitably administered bid, with twice daily dosing being most preferred for doses of 300 mg and above.

[0273] One advantage of the present invention, and a difference from the EBRT approach to delivering radiation therapy, is that the radiation therapy agent does not need to be administered every day of the treatment protocol. Thus, in any embodiment of the present invention, the radiation therapy agent may be administered at intervals of about once a week, about once every two weeks, about once every three weeks, about once every four weeks, or at longer intervals between administrations. In a preferred embodiment, the radiation therapy agent is administered twice, 7 days or more, 10 days or more, 14 days or more, 21 days or more, or 28 days or more, or three times, 7 days or more, 10 days or more, 14 days or more, 21 days or more, or 28 days or more, apart. It will be understood that additional administrations may be required. In certain embodiments, only a single administration of the radiation therapy agent may be required.

[0274] Pharmaceutical compositions containing one or more agents (e.g., radioimmunoconjugates and / or DNA-PKi) can be formulated into a variety of drug delivery systems for use according to the disclosed methods and systems. For appropriate formulation, one or more physiologically acceptable excipients or carriers can also be included in the composition. Examples of suitable formulations can be found in Remington's Pharmaceutical Sciences, Mack Publishing Company, Philadelphia, PA, 17th ed., 1985. For a brief review of methods for drug delivery, see, e.g., Langer (Science 249:1527-1533, 1990).

[0275] Compositions comprising M3814 suitable for oral administration are described in WO 2018 / 178134, which is incorporated herein by reference.

[0276] kit The present invention further provides kits containing one or more components for use in the therapeutic methods described herein.

[0277] Preferably, the kit comprises a molecularly targeted radiotherapeutic agent and a DNA-PK inhibitor for simultaneous, separate or sequential use in the treatment of a hyperplastic or neoplastic disease as described herein.

[0278] The kit may include a container (e.g., a bottle) in which a mixture of the two components reside, or the kit may include two separate containers, each containing one of the two components.

[0279] Sequence information [Table 1-1] [Table 1-2] [Table 1-3] [Table 1-4] [Table 1-5] [Table 1-6]

[0280] [Table 2-1] [Table 2-2] [Table 2-3]

Table 2-4

Table 2-5

Table 2-6

Table 2-7

Table 2-8

Table 2-9

Table 2-10

Table 2-11

Table 2-12

Table 2-13

Table 2-14

Table 2-15

[0281] Example 1: In a metastatic renal cell carcinoma xenograft model 177 In vivo efficacy of Lu-anti-CAIX antibody + DNA-PKi Established SK-RC-52 xenografts (approximately 100 mm 3 Female BALB / c nude mice bearing the BALB / c IgG antibody were used in this study. The mice were divided into one of three groups: Vehicle control (oral) n=4 · 177 Lu-anti-CAIX antibody alone (DOTA-GmAb described herein, 162 μCi / 6 MBq) n=4 Orally administered 50 mg / kg M3814 DNA-PKi, and 177 Lu-anti-CAIX antibody (162μCi / 6MBq)n=4

[0282] The animals were treated as follows. Day -14: 60 female mice were injected with RK-RC-52 metastatic RCC cells in Matrigel. Day 0: 177 Lu-anti-CAIX antibody was administered intravenously, as a single dose, once only Days 1-7: Vehicle / M3814 administered orally at 50 mg / kg daily

[0283] SPECT / MRI imaging was performed on the 3rd and 6th days to examine biodistribution.

[0284] Tumor growth curves were monitored in mice for up to 6 months or until ethical limits.

[0285] FIG. 1 shows the results of 177 The results of SPECT imaging of Lu-anti-CAIX antibody are shown. 177 A single administration of Lu-anti-CAIX antibody delivers cytotoxic radiation specifically to the tumor for an extended period of time.

[0286] Figure 2 shows the tumor volume (mm 3 ) and the rate of change in tumor volume are shown. 177 Mice treated with Lu-anti-CAIX antibody and M3814 had significantly smaller tumor sizes after 143 days compared to mice treated with antibody alone.

[0287] [Table 3]

[0288] Example 2: PSMA high In prostate cancer xenograft models 177 In vivo efficacy of Lu-anti-PSMA antibody + DNA-PKi Established LNCaP xenografts (approximately 200 mm 3 Male BALB / c nude mice (5-6 weeks old) bearing the BALB / c IgG antibody were used in this study. The mice were divided into one of three groups: Vehicle control (oral) n=3 · 177 Lu-anti-PSMA antibody alone (DOTA-HuJ501 as described herein, 162 μCi / 6 MBq) n=4 Orally administered 50 mg / kg M3814 DNA-PKi and 177 Lu-anti-PSMA antibody (162μCi / 6MBq)n=4

[0289] The animals were treated as follows. Day -20: 5 × 10 6 Inject LNCaP cells Day 0: 177 Lu-anti-PSMA antibody administered intravenously, as a single dose, once only Days 1-14: Vehicle / M3814 administered orally at 50 mg / kg daily

[0290] SPECT / MRI imaging was performed on the 3rd and 7th days to examine biodistribution.

[0291] Tumor growth curves were monitored in mice for up to 6 months or until ethical limits.

[0292] FIG. 3 shows the results of the mouse 177 The results of SPECT imaging of Lu-anti-PSMA antibody are shown. 177 Mice treated with Lu-anti-PSMA antibody and M3814 had significantly smaller tumors 112 days after the start of treatment compared to mice treated with antibody alone.

[0293] FIG. 4 shows the tumor volume (mm 3 ) and the rate of change in tumor volume are shown.

[0294] [Table 4]

[0295] Example 3: 177 Clinical trial of combination of Lu-direntuximab and peposertib (M3814) with CAIX-expressing renal tumors the purpose Main purpose: Combined with peposertib 177 Determine the maximum tolerated dose (MTD) of Lu-Direntuximab Treatment-emergent adverse events (TEAEs): will be assessed for type per Medical Dictionary for Regulatory Activities (MedDRA), frequency, severity per NCI CTCAE v.5.0, seriousness, and relationship to study treatment. Laboratory abnormalities will be assessed according to NCI CTCAE v.5.0 Events.

[0296] Secondary Objectives: - RECIST v1.1 for combination peposertib 177 Overall response rate of Lu-direntuximab - Estimate progression-free survival (PFS) - Estimate overall survival (OS) Estimate response duration -Evaluate the safety and tolerability of combination therapies Quality of life determined using the EORTC QLQ-C30 questionnaire

[0297] Exploratory purpose: Correlation between PFS and CAIX expression Correlation between OS and CAIX expression Zirconium-89( 89 Zr)-direntuximab will be used to assess radiation response by PET / CT. - Whole body planar imaging and SPECT were used to 177 Distribution, lesion uptake, and dosimetry assessment of Lu-labeled dilentuximab will be assessed and results will be correlated with patient outcomes (e.g., response, PFS).

[0298] method: Phase I Study Design: Administered intravenously in combination with peposertib in patients with relapsed / refractory CAIX-expressing renal tumors 177 An open-label, single-arm, randomized, parallel-group, multicenter dose-finding study will be conducted to evaluate escalating radioactive dose levels of Lu-dilentuximab.

[0299] The protocol began with a safety run-in using a 3+3 design and included combination therapy with peposertib at up to 400 mg twice daily from day 1 until progression as determined by PET imaging. 177 To establish the maximum tolerated dose (MTD) of 11U-labeled dilentuximab. 177 The initial dose of Lu-labeled dilentuximab was 1110MBq / m 2 , which is less than 50% of the single agent dose established in previous studies, and proceeds as shown in the following schema: Once the MTD is established, Simon's two-stage optimal design begins.

[0300] Ten patients will be enrolled in Phase 1, and the study will be terminated if no response is observed. Patients treated at the MTD during the safety lead-in period will be included in Phase II. The total number of patients in the safety lead-in and Phase II is 60. [Table 5]

[0301] or, 177 Lu-labeled dilentuximab and peposertib were administered according to the following doses: 177 Lu-labeled dilentuximab will be administered three times at 3-week intervals. [Table 6]

[0302] All patients undergo standard of care imaging, including FDG PET scan, CT, or MRI, which are used to assess the extent of disease.

[0303] All patients 177 Before each administration of Lu-Direntuximab 89 Underwent Zr-dilentuximab PET / CT scan 177 After each dose of Lu-dilentuximab 177 A Lu whole-body (WB) planar scan and SPECT / CT scan will be performed. To be eligible for the study, 89 Zr-direntuximab PET / CT positive (at least one metastatic lesion 89 However, the second and third 177 Prior to administration of Lu-direntuximab, 89 A positive Zr-direntuximab PET / CT is not required. The first 10 patients treated at the MTD and who consent to additional imaging will undergo three whole-body planar scans (0-4 h, 48-72 h ± 6 h, and 96-144 h ± 6 h) and a SPECT / CT scan at 48-72 h (± 6 h) after administration of the first dose of 177Lu-direntuximab.

[0304] In the first 10 patients 177 Additional doses of Lu-direntuximab (second and third doses) and the remaining patients 177For all doses of Lu-direntuximab, 177 One WB planar scan and one SPECT / CT will be performed 48-72 hours (+ / - 6 hours) after each dose of Lu-direntuximab, as summarized in Table 1 below. [Table 7]

[0305] Suggested Dosing Schedule: Day 1: 177 7Lu-Direntuximab Days 2 to 15: Peposertib Days 16-21: Treatment is suspended Day 22: 177 Lu-Direntuximab Days 23 to 37: Peposertib Days 38-45: Treatment is discontinued Day 46: 177 Lu-Direntuximab Days 47-61: Peposertib

[0306] Inclusion criteria: Patients who meet all of the following criteria at screening are eligible to participate in the study: 1. Histologically confirmed renal cell carcinoma previously treated with all approved standard therapies. 2. Zirconium-89( 89 At least one evaluable CAIX-positive metastatic lesion in renal tissue defined by RECIST 1.1 on Zr)-direntuximab PET / CT. 3. Age 18 or older. 4. Karnofsky performance status ≥70. 5.Has sufficient organ function as follows at screening: · Bone marrow: White blood cells ≥ 3,000 / mL, absolute neutrophil count ≥ 1,500 / mL, platelets ≥ 100,000 / mL, hemoglobin ≥ 9 g / dL. AST, ALT, and alkaline phosphatase ≤ 2.5 x ULN, with the following exceptions: a) Patients with documented liver metastases: AST and / or ALT ≤ 5xULN b) Patients with documented liver or bone metastases: alkaline phosphatase > 5 Below ULN Serum bilirubin ≤2xULN (patients with known Gilbert's disease and serum bilirubin ≤3xULN may be enrolled) -INR and aPTT ≤ 1.5 x ULN (only applicable to patients not receiving therapeutic anticoagulant therapy; patients receiving therapeutic anticoagulant therapy must continue taking the drug) 6.Able to understand the study and able and willing to comply with all protocol requirements. 7. Ability to receive and maintain oral medications. 8. Follow radiation protection guidelines applied by the treatment institution to protect patient contacts and the general public (including hospitalization and isolation). 9. Agree to take adequate precautions to prevent pregnancy to avoid potential problems related to radiation exposure to the fetus (see Clinical Trials Facilitation Group, 2020: Recommendations related to contraception and pregnancy testing in clinical trials Version 1.1, CTFG, 2020).

[0307] Exclusion criteria: Patients who meet any of the following criteria are not eligible to participate in the study: previous treatment with 177Lu-direntuximab, known hypersensitivity to direntuximab or the DOTA linker; exposure to murine or chimeric antibodies within the past 5 years; administration of medicines / herbal supplements known to be potent inhibitors or inducers of CYP3A or CYP2C19; previous administration of any radionuclide with a half-life of 10 or less; history of need for steroids >10 mg prednisone per day for the past 2 years for autoimmune comorbidities; anticancer therapy within 2 weeks prior to enrollment; history of severe allergic, anaphylactic, or other hypersensitivity reactions to chimeric or humanized antibodies or fusion proteins; history of HIV infection, ongoing or chronic hepatitis B or C infection; New York Heart Association heart disease (class II or higher), myocardial infarction within the past 3 months, unstable arrhythmia, or unstable angina. Patients must be on a stable medical regimen optimized in the opinion of the treating physician, with consultation with a cardiologist as necessary; history of stroke or transient ischemic attack within 6 months prior to Day 1 of Cycle 1; significant vascular disease (e.g., aortic aneurysm requiring surgical repair or recent peripheral arterial thrombosis) within 6 months prior to Day 1 of Cycle 1; evidence of bleeding diathesis or significant coagulopathy (in the absence of therapeutic anticoagulation); clinical signs or symptoms of gastrointestinal obstruction or the need for routine parenteral hydration, parenteral nutrition, or tube feeding; severe refractory or dehiscing wounds, ongoing ulcers, or untreated fractures; major surgery within 4 weeks prior to enrollment (biopsy or line placement may be performed up to 24 hours prior to enrollment); pregnant and lactating women.

[0308] Investigational drug, dose, and mode of administration: It is a chimeric monoclonal antibody (INN name: direntuximab (GTX), alias: cG250, TLX250) specific to the CAIX (carbonic anhydrase 9) antigen, radiolabeled with positrons that release the radioactive metal ruthenium-177 via a DOTA linker. 177 Lu-TLX250.

[0309] Treatment duration: Approximately 9 weeks or approximately 36 weeks.

[0310] Patients' study participation period: Patients are expected to participate in the study for up to 36 weeks or up to 58 weeks.

[0311] Concomitant therapy, dosage, and mode of administration: Not applicable. Supportive care is acceptable.

[0312] Example 4: In patients with prostate-specific membrane antigen (PSMA) expressing metastatic castration-resistant prostate cancer (mCRPC) 177 Clinical trial of combination of Lu-rosopatamab and peposertib the purpose Main purpose Phase I: Combined with peposertib 177 Lu-rosopatamab ( 177 To determine the maximum tolerated dose (MTD) of Lu-J591 -Incidence of treatment-emergent adverse events Phase II: Combined with peposertib 177 Objective response rate of Lu-losopatamab

[0313] Secondary Objectives: Biochemical response as indicated by PSA levels, changes in tumor-free circulating DNA (ctDNA), alkaline phosphatase (ALP), CD4 / CD8 subset analysis, and lactate dehydrogenase (LDH) levels Biological progression-free survival Radiographic progression-free survival ·Overall survival Best overall response rate (BORR) defined by RECIST criteria Safety profile

[0314] method: Phase I Study Design It is administered intravenously in combination with peposertib in relapsed / refractory patients with PSMA-expressing mCRPC 177 This is an open-label, single-arm, randomized, parallel-group, multicenter dose-finding study to evaluate escalating radioactive dose levels of Lu-rosopatamab. 177 Lu-losopatamab is administered as two doses, 14 days apart; or 177 Lu-losopatamab may be administered in two doses, six weeks apart.

[0315] Patients will be treated in cohorts according to a 3+3 study design at dose levels defined below.

[0316] Peposertib will be administered daily at up to 400 mg twice daily from day 1 until progression as determined by PET imaging.

[0317] The dose of peposertib, 400 mg twice daily, will remain the same unless there is dose-limiting toxicity.

[0318] In the event of dose-limiting toxicity at the first dose, 177 Dose tapers of Lu-losopatamab and peposertib may be performed. [Table 8]

[0319] Alternatively, administration can be as follows. [Table 9]

[0320] Before moving to the next dose level, a safety review committee will review the safety data from the current dose level for the first cycle.

[0321] Phase 2 Study Design: Thirty-two patients were enrolled at the recommended dose determined in the Phase 1 component of this study. 177Treat with Lu-rosopatamab with peposertib at the recommended dose until progression.

[0322] (Planned) Number of Patients: Phase 1: Dose finding: Estimated 12-18 patients Patients will be treated in cohorts according to a 3+3 study design, with at least four dose escalation cohorts and an optional dose de-escalation cohort.

[0323] Phase 2: Preliminary efficacy: 32 patients Patients will receive 100 mg of ... 177 The patient will receive Lu-rosopatamab and peposertib.

[0324] Suggested Dosing Schedule: Day 1: 177 Lu-rosopatamab Days 2 to 22: Peposertib Days 23 to 60: Treatment is suspended Day 61: 177 Lu-rosopatamab Days 62 to 82: Peposertib

[0325] Inclusion criteria: Patients who meet all of the following criteria at screening are eligible to participate in the study: 1. Histologically confirmed mCRPC that has relapsed / refractory to all standard therapies. 2. PSMA positivity as defined by PET imaging with Ga-PSMA. 3. Age 18 or older. 4. Karnofsky performance status ≥60. 5.Has sufficient organ function as follows at screening: · Bone marrow: White blood cells ≥ 3,000 / mL, absolute neutrophil count ≥ 1,500 / mL, platelets ≥ 100,000 / mL, hemoglobin ≥ 9 g / dL. Liver function: Total bilirubin ≤ 1.5 x upper limit of normal (ULN). For patients with known Gilbert's syndrome, ≤ 3 x ULN is acceptable. Alanine aminotransferase (ALT) or aspartate aminotransferase (AST) ≤ 2.5 x ULN. Renal function Serum / plasma creatinine ≤1.5 × ULN or creatinine clearance ≥50 mL / min. 6.Able to understand the study and able and willing to comply with all protocol requirements. 7.Able to understand the study and able and willing to comply with all protocol requirements. 8. Have the ability to receive and maintain oral medications. 9. Follow radiation protection guidelines applied by the treatment institution to protect patient contacts and the general public (including hospitalization and isolation). 10. Agree to take adequate precautions to prevent pregnancy for your partner to avoid potential problems related to radiation exposure to the fetus (see Clinical Trials Facilitation Group, 2020: Recommendations related to contraception and pregnancy testing in clinical trials Version 1.1, CTFG, 2020).

[0326] Exclusion criteria: Patients who met any of the following criteria were not eligible to participate in the study: unable to undergo MRI or PET imaging; 177Previous lutetium-based treatment (external radiation is permitted); administration of medicinal / herbal supplements known to be potent inhibitors or inducers of CYP3A or CYP2C19; history or evidence of delayed-type hypersensitivity (DTH)-dependent chronic infections (e.g., tuberculosis, systemic fungal infections, or systemic parasitic infections) that may worsen under systemic corticoid treatment; known allergy to any non-active ingredients in the study drug or to any other intravenously administered human protein / peptide / antibody; hemostatic conditions that would preclude catheterization or invasive treatment; chronic renal impairment as indicated by a creatinine clearance of less than 45 mL / min or a serum creatinine of more than 1.5 ULN; any significant comorbidities that may render participation undesirable or compromise compliance with the protocol; patients with an ongoing medically documented history of major depressive episode, bipolar disorder (type I or type II), obsessive-compulsive disorder, schizophrenia, history of suicide attempts or ideation, or history of homicidal ideation (e.g., risk of harming self or others), or ongoing severe personality disorder; major trauma, including major surgery (abdominal / cardiac / thoracic surgery, etc.) within 3 weeks of administration of study treatment; pregnancy or breastfeeding; the need for chronic administration of high-dose corticosteroids or other immunosuppressants; subjects must have been off corticosteroids or been taking a stable or reduced dose of dexamethasone (or equivalent) ≤ 4 mg / day for 7 days prior to the start of chemoradiation therapy. Limited or occasional use of corticosteroids to treat or prevent acute adverse reactions is not considered an exclusion criterion. HIV-positive participants on combination antiretroviral therapy are ineligible due to potential pharmacokinetic interactions with bavituximab. Furthermore, these participants are at high risk for fatal infections if treated with myelosuppressive therapy. If indicated, appropriate studies will be performed in participants receiving combination antiretroviral therapy.The presence of ongoing uncontrolled infection or other serious intercurrent illness that, in the opinion of the investigator, would expose the patient to undue risk or interfere with the study; concurrent malignancy, unless the patient has been disease-free for 2 years or more without the intervening serious non-healing wound, ulcer, or fracture; the need for concurrent use of other anticancer treatments or medications other than the study drug; supportive care is acceptable; any recent live vaccination within 4 weeks prior to treatment or plans to receive a vaccination during the study.

[0327] Treatment duration: Approximately 36 weeks.

[0328] Patients' study participation period: Patients are expected to participate in the study for up to 58 weeks.

[0329] Concomitant therapy, dosage, and mode of administration: Not applicable. Supportive care is acceptable.

[0330] Evaluation criteria: Primary endpoints of Phase 1: Combined with peposertib 177 To determine the maximum tolerated dose (MTD) of Lu-rosopatamab Treatment-emergent adverse events (TEAEs): will be assessed for type, frequency per MedDRA (Medical Dictionary for Regulatory Activities), severity per NCI CTCAE v.5.0, seriousness, and relationship to study treatment. Laboratory abnormalities will be assessed according to NCI CTCAE v.5.0 Events.

[0331] Secondary endpoints of Phase 1 Whole-body biodistribution and dosimetry (safety dosimetry) Residence time in identifiable organs (MBq*h) Organ absorbed dose and whole body absorbed dose (μGy / MBq) After systemic administration 177 Tumor dosimetry (therapeutic dosimetry) of Lu-losopatamab Cmax (maximum active concentration in the tumor, Bq / cm3 ) tmax (time point of maximum active concentration in the tumor) Tumor absorbed dose (μGy / MBq) Absorbed radiation dose to kidneys, liver, lungs, spleen, bone marrow / red bone marrow, and gastrointestinal tract (administered 177 Lu-rosopatamab) and below the acceptable safety limit defined by ARPANSA (see Radiation Risk Assessment).

[0332] Primary endpoints of Phase 2: Overall response rate as defined by RECIST

[0333] Secondary endpoints of Phase 2: Biochemical response as indicated by PSA levels, changes in tumor-free circulating DNA (ctDNA), alkaline phosphatase (ALP), CD4 / CD8 subset analysis, and lactate dehydrogenase (LDH) levels Biological progression-free survival Radiographic progression-free survival ·Overall survival Best overall response rate (BORR) defined by RECIST criteria Safety profile

[0334] Example 5: 177 Clinical trial of combination of Lu-direntuximab and peposertib (M3814) with CAIX-expressing metastatic or unresectable ccRCC method: All patients 177 Prior to each dose of Lu-direntuximab 89 To be eligible for this study, patients must undergo a baseline Zr-dilentuximab PET / CT scan. 89 The Zr-TLX250CDx PET / CT had to be positive, i.e., ≥75% of the total lesion area or volume 89 The uptake of Zr-dilentuximab was significantly greater than in normal liver (i.e., standardized uptake value [SUV] max(SUV must be ≥ 1.5 times the normal liver SUV).

[0335] 177 For patients eligible for retreatment with Lu-dilentuximab and peposertib (i.e., cycles 2 and 3, or later if responding), a positive ≥ 10 day(s) prior to retreatment was required. 89 A Zr-dilentuximab PET / CT scan should also be obtained. Patients will undergo tumor assessment and evaluation per RECIST 1.1 criteria. Contrast-enhanced CT and / or MRI of the chest, abdomen, and pelvis will be performed during screening (within 4 weeks prior to day 1 of cycle 1), then every 8 weeks for the first 6 months, and every 12 weeks for the following 6 months. After the first year, patients should be scanned every 6 months. At these time points, patients will undergo further FDG-PET as clinically indicated.

[0336] Patients who discontinue study treatment for reasons other than disease progression will be required to follow this evaluation schedule until they experience disease progression, start a new anticancer therapy, or are lost to follow-up.

[0337] Part 1 is 177 The study evaluates the combination of three different activities of Lu-dilentuximab with three different dose levels of peposertib. Patients with CAIX-positive renal cancer will be enrolled at a given dose level in cohorts of 2-4 patients (three patients at the starting dose level). At the initial dose level, patients will receive 1887MBq of activity (standard 1.7m 2 1110MBq / m in an adult individual 2 (equivalent to the dose) 177 The patient will receive Lu-direntuximab.

[0338] Cycles 1 to 3 are in combination with peposertib at a dose of 150 mg, twice daily (D4 to D21). Treatment cycles have a fixed duration of 84 days. Patients are treated for up to three doses or until clinically significant progression or unacceptable toxicity. 177The dose of Lu-direntuximab will be given at 75% of the activity of the previous cycle. Responding patients may receive treatment every 84 days from cycle 3 onwards.

[0339] Phase I Study Design: 177 Lu-direntuximab will be administered at the following dose levels / activity: [Table 10]

[0340] Peposertib will be administered at the following dose levels: [Table 11]

[0341] At the start of the titration phase, patients will receive dose level A2, i.e., 1887 MBq in combination with peposertib at 150 mg twice daily.

[0342] After each dose level is completed, the SRC, with the support of the PO-BLRM output, will decide which of the study drugs will remain unchanged, be escalated, or be tapered to the next dose level. Simultaneous escalation of both study drugs is not permitted.

[0343] Depending on the safety and / or efficacy data, the SRC may recommend testing additional dose schedules, e.g., delaying the initiation of peposertib until day 7 or shortening the duration of peposertib administration.

[0344] Patient treatment, assessment of disease progression, and inclusion and exclusion criteria were the same as in Example 3.

[0345] Proposed Dosing Schedule (cycles are fixed at 84 days duration) Cycle 1, Cycle 2, and Cycle 3: Day -28 to Day -1: 177Lu-Direntuximab Imaging + CT / MRI + / -FDG-PET Day 1: 177 Lu-Direntuximab Injection Days 4-21: Peposertib PO, twice daily Days 22-84: Treatment is suspended

[0346] Investigational drug, dose and mode of administration: It is a chimeric monoclonal antibody (INN name: direntuximab (GTX), alias: cG250, TLX250) specific to the CAIX (carbonic anhydrase 9) antigen, radiolabeled with positrons that release the radioactive metal ruthenium-177 via a DOTA linker. 177 Lu-TLX250( 177 Lu-DOTA-direntuximab).

[0347] 1700-4080MBq in 10mL 177 Contains Lu-TLX250, which is 177 The proposed single clinical dose of Lu-TLX250 is 2405MBq / m 2 up to 10 mg total antibody mass dose upon administration.

[0348] The non-radioactive portion is the immunoconjugate DOTA-direntuximab with a total antibody dose of 10 mg (i.e., there will be no unconjugated direntuximab). 177 Lu-TLX250( 177 Lu-DOTA-direntuximab) is intended for IV administration via slow IV push. 177 The Lu-direntuximab product contains 1700-4080MBq of 177Lu-direntuximab in 10mL, which provides a patient dose of 2405MBq / m 2 up to 10 mg total antibody mass dose upon administration. The injectate has high radiochemical purity (>90%) and contains less than 10% of both 177Lu and 177Lu-DOTA.

[0349] The intended dosing schedule is planned as up to three repeated doses administered every 84 days (day 1 of cycles 1-3), with subsequent doses being 75% of the previous dose. This dosing regimen has an MTD of 2405 MBq / m 2 This is based on previous clinical trials which determined that subsequent doses at 75% of the previous dose were well tolerated. Although a maximum of three doses are intended to be administered in total, each of the three doses can be considered a single dose, as the interval between doses will exceed four weeks.

[0350] The accumulation of chemotoxicity associated with DOTA-direntuximab cannot be predicted from dosing intervals of 12 to 14 weeks apart.

[0351] Peposertib (M3814), with the chemical name (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-ylquinazolin-4-yl)-phenyl]-(6-methoxy-pyridazin-3-yl)-methanol, is a potent and selective small molecule adenosine triphosphate competitive inhibitor of DNA-PK that targets tumor cell proliferation and survival by inhibiting key DNA damage repair mechanisms in solid tumors and hematological malignancies. For this clinical trial, a peposertib film-coated tablet containing 50 mg of drug substance is available. The peposertib film-coated tablet represents the formulation for oral administration.

[0352] 89 Zr-dilentuximab is an investigational drug and will be supplied as a ready-to-inject solution. 89 Diagnostic administration of Zr-direntuximab followed by 89 Studies will be required to include non-therapeutic evaluations, including PET CT scans 4–7 days after Zr-direntuximab. 89 Zr-direntuximab binds to the lysine residues of GTX, 89 Positron-emitting radioactive metal zirconium-89 (NSuc-DFO-TFPester (DFO-TFP)) was synthesized to generate Zr-DFOTFP-GTX. 89It is a chimeric monoclonal antibody (INN name: direntuximab (GTX), alias: cG250, TLX250) radiolabeled with 1Zr and specific for the CAIX (carbonic anhydrase 9) antigen.

[0353] 37MBq (±10%) containing a mass dose of 10mg dilentuximab 89 A single dose of Zr-TLX250 will be administered by slow intravenous (IV) infusion over a minimum of 3 minutes. Safety assessments will be performed before and after administration. 89 Zr-dilentuximab will be prepared in glass vials as a solution for intravenous administration at a nominal dose strength of 37 MBq (±10%) for single intravenous use. The dose level selected is based on previous safety, biodistribution, and dosimetry findings from Phase I studies.

[0354] 89 The Zr-direntuximab formulation is manufactured in a "ready-to-use" form. No dietary restrictions prior to administration are required. A whole-body PET / CT scan (from skull base to mid-thigh) is acquired at a single time point 4-7 days after administration of 89Zr-direntuximab, using 6-8 bed positions, with an acquisition time of 5-10 minutes per bed position, using low-dose CT. 89 Zr-direntuximab standard uptake values ​​(SUV) are determined for each tumor lesion. Scans are performed at baseline and before subsequent 177Lu-direntuximab administrations (e.g., at approximately C2D1 and C3D1).

[0355] Treatment period The approximately 7-month follow-up will be approximately 6 months after completion of treatment.

[0356] It will be understood that the invention disclosed and defined herein extends to all alternative combinations of two or more of the individual features mentioned or apparent from the text or drawings, all of these different combinations constituting various alternative aspects of the invention.

Claims

1. i) a DNA-PK inhibitor (DNA-PKi), ii) molecularly targeted radiotherapeutic agents capable of cellular internalization and / or retention in the blood circulation of a subject; Including, the molecularly targeted radiotherapeutic agent comprises a radionuclide that is a beta emitter; Optionally, the molecularly targeted radiotherapeutic agent and the DNA-PKi are administered sequentially in any order or simultaneously; A pharmaceutical combination for use in treating a disease or disorder characterized by abnormal cell proliferation and function in a subject.

2. 2. The combination of claim 1, wherein the molecularly targeted radiotherapeutic agent is a radioimmunoconjugate, preferably an antibody or antigen-binding fragment thereof conjugated to a radionuclide for binding to an antigen associated with the disease or disorder in need of treatment.

3. 2. The combination of claim 1, wherein the molecularly targeted radiotherapeutic agent is an antibody for binding to an antigen associated with the disease or disorder in need of treatment, the antibody being conjugated to a radionuclide, preferably the antibody is an immunoglobulin selected from IgG1, IgG2, IgG3, and IgG4, particularly an antibody that undergoes predominantly hepatic clearance.

4. 2. The combination of claim 1, wherein the disease or disorder is cancer, the molecularly targeted radiotherapeutic agent comprises an antibody or antigen-binding fragment thereof for binding to a tumor-associated or tumor-specific antigen expressed by the cancer, and optionally, the cancer is metastatic cancer.

5. 2. The combination of claim 1, wherein the disease or disorder is a non-cancerous proliferative cell disorder and the molecularly targeted radiotherapeutic agent comprises an antibody or antigen-binding fragment thereof for binding to an antigen expressed by the proliferative cells.

6. the disease or disorder being treated is a cancer characterized by expression of carbonic anhydrase IX (CAIX), and the molecularly targeted radiotherapeutic agent comprises an antibody or antigen-binding fragment thereof capable of specifically binding to CAIX; or The disease or disorder to be treated is a cancer characterized by expression of prostate-specific membrane antigen (PSMA), and the molecular targeted radiotherapeutic agent comprises an antibody or an antigen-binding fragment thereof capable of specifically binding to PSMA. The combination according to claim 4.

7. The DNA-PKi is i) (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharmaceutically acceptable salt thereof; the molecularly targeted radiotherapeutic agent is ii) an antibody or antigen-binding fragment thereof for binding to CAIX, wherein the antibody or antigen-binding fragment thereof is conjugated to a radionuclide for delivering a radiotherapeutic dose to the cancer; 2. The combination of claim 1, the cancer is characterized by expression of CAIX, and optionally the cancer is selected from the group consisting of renal cell carcinoma (including clear cell renal cell carcinoma), colon cancer, breast cancer, lung cancer, cervical cancer, and melanoma, preferably the cancer is kidney cancer; optionally, the cancer is metastatic cancer, optionally metastatic renal cell carcinoma; The above combination.

8. The antibody or antigen-binding fragment for binding to CAIX is a) a G250 antibody or an antibody comprising an antigen-binding domain as defined in Table 2 herein, or b) an antibody or antigen-binding fragment comprising an antigen-binding domain, wherein the antigen-binding domain is i) a heavy chain variable region comprising: a CDR1 comprising the amino acid sequence set forth in any one of SEQ ID NOs: 49, 65, 81, 97, and 113; a CDR2 comprising the amino acid sequence set forth in any one of SEQ ID NOs: 50, 66, 82, 98, and 114; and a CDR3 comprising the amino acid sequence set forth in any one of SEQ ID NOs: 51, 67, 83, 99, and 115; and ii) a light chain variable region comprising: CDR1 comprising the amino acid sequence set forth in any one of SEQ ID NOs: 129, 145, 161, 177, 193, and 209; CDR2 comprising the amino acid sequence set forth in any one of SEQ ID NOs: 130, 146, 162, 178, 194, and 210; and CDR3 comprising the amino acid sequence set forth in any one of SEQ ID NOs: 131, 147, 163, 179, 195, and 211. or c) an antibody or antigen-binding fragment comprising an antigen-binding domain, wherein the antigen-binding domain comprises a heavy chain variable region comprising an amino acid sequence that is 80% or more identical to the amino acid sequence set forth in any one of SEQ ID NOs: 52, 68, 84, 100, and 116, and a light chain variable region comprising an amino acid sequence that is 80% or more identical to the amino acid sequence set forth in any one of SEQ ID NOs: 132, 148, 164, 180, 196, and 212; or d) An antibody comprising the amino acid sequence set forth in any one of SEQ ID NOs: 225 to 228, preferably in combination with SEQ ID NO: 229, and most preferably in combination with SEQ ID NO: 231 and SEQ ID NO:

234.

8. The combination of claim 7, comprising:

9. The method of claim 8, wherein the DNA-PKi is i) (S)-[2-chloro-4-fluoro-5-(7-morpholin-4-yl-quinazolin-4-yl)-phenyl]-(6-methoxypyridazin-3-yl)-methanol (M3814) or a pharmaceutically acceptable salt thereof; the molecularly targeted radiotherapeutic agent is ii) an antibody or antigen-binding fragment thereof for binding to PSMA, wherein the antibody or antigen-binding fragment thereof is conjugated to a radionuclide for delivering a radiotherapeutic dose to the cancer; 2. The combination of claim 1, the cancer is characterized by expression of PSMA, and optionally the cancer is selected from the group consisting of prostate cancer, bladder cancer, testicular embryonal carcinoma, neuroendocrine cancer, renal cell carcinoma, and breast cancer, preferably the cancer is prostate cancer; Optionally, the cancer is metastatic prostate cancer, optionally metastatic castration-resistant prostate cancer (mCRPC); The above combination.

10. the antibody or antigen-binding fragment for binding to PSMA is a) the J591 antibody or a variant or humanized form thereof, or b) an antibody comprising an antigen-binding domain as defined in Table 1 herein; or c) i) a heavy chain variable region comprising a CDR1 comprising the amino acid sequence set forth in any one of SEQ ID NOs: 1, 17, or 244, a CDR2 comprising the amino acid sequence set forth in any one of SEQ ID NOs: 2 or 18, and a CDR3 comprising the amino acid sequence set forth in any one of SEQ ID NOs: 3 or 19; and ii) a light chain variable region comprising a CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 33, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 34, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 35; or an antibody or antigen-binding fragment comprising d) an antibody or antigen-binding fragment comprising an antigen-binding domain, wherein the antigen-binding domain comprises a heavy chain variable region comprising an amino acid sequence that is 80% or more identical to the amino acid sequence set forth in any one of SEQ ID NO: 4 and SEQ ID NO: 20, and a light chain variable region comprising an amino acid sequence that is 80% or more identical to the amino acid sequence set forth in SEQ ID NO: 36; or e) An antibody comprising the amino acid sequence set forth in any one of SEQ ID NOs: 239 to 242, preferably comprising the amino acid sequences of SEQ ID NOs: 239 and 243.

10. The combination of claim 9, comprising:

11. The radionuclide is a beta or beta / gamma emitter, preferably lutetium- １７７ ( １７７ Lu), yttrium- ９０ ( ９０ Y), iodine- １３１ ( １３１ l), samarium- １５３ ( １５３ Sm), holmium- １６６ ( １６６ Ho), rhenium- １８６ ( １８６ Re), and rhenium- １８８ ( １８８ 2. The combination of claim 1, wherein the radionuclide is selected from the group consisting of lutetium- 177 ( 177 Lu) or rhenium- 188 ( 188 Re).

12. The combination of claim 1, wherein the molecularly targeted radiotherapeutic agent and the DNA-PKi are administered sequentially in any order or simultaneously.

13. The DNA-PKi, preferably M3814, is administered after the administration of the molecularly targeted radiotherapeutic agent. Optionally, the DNA-PKi is administered for 1 or more days, 2 or more days, 3 or more days, 4 or more days, 5 or more days, 6 or more days, 7 or more days, or more, following administration of the molecularly targeted radiotherapy agent; Optionally, the DNA-PKi is administered for 7 days or less, 6 days or less, 5 days or less, 4 days or less, 3 days or less, 2 days or less, or 1 day or less after administration of the molecularly targeted radiotherapy drug; Optionally, the DNA-PKi is administered within 24 hours after administration of the molecularly targeted radiotherapy drug. The combination of claim 1.

14. 10. The combination of claim 1, wherein the molecularly targeted radiotherapeutic agent is administered as a single dose to treat a disease or disorder characterized by abnormal cell proliferation or function in the subject.

15. The molecularly targeted radiotherapy agent is administered in one or more treatment cycles, each treatment cycle comprising administration of the molecularly targeted radiotherapy agent followed by administration of the DNA-PKi, preferably M3814, for a period of 7 days or more, 14 days or more, 21 days or more, or more. Optionally, there is a treatment break between treatment cycles, preferably the treatment break is 7 days or more, 14 days or more, 21 days or more, 28 days or more, 35 days or more, 42 days or more, 49 days or more, 56 days or more, 63 days or more, or more, more preferably the treatment break is 100 days or less; optionally, the dose of the radiotherapeutic agent for administration is about 25% or more, about 30% or more, about 35% or more, about 40% or more, about 45% or more, or about 50% or more less than the therapeutic dose required for monotherapy with said radiotherapeutic agent; Optionally, the dose of said DNA-PKi, preferably the dose of M3814, for administration is at a dose level below the maximum tolerated dose level, optionally at a dose of 90% or less, 85% or less, 80% or less, 75% or less, 60% or less, 65% or less, 60% or less, or 55% or less of said maximum tolerated dose level, and / or at a dose of 10% or more, 20% or more, 30% or more, 40% or more, or 50% or more of the maximum tolerated dose level of said combination; The combination of claim 1.

16. iii) further comprising an additional anti-cancer therapeutic agent selected from the group consisting of an immune checkpoint modulator, a chemotherapeutic agent, and a radiosensitizer; Optionally, the immune checkpoint modulator is selected from an inhibitor of PD-1, an inhibitor of PD-L1, and an inhibitor of CTLA-4, or any other immune checkpoint inhibitor described herein. Preferably, the inhibitor of PD-1 is selected from pembrolizumab, nivolumab, cemiplimab, spartalizumab, canrelizumab, sintilimab, tislelizumab, toripalimab, dostarimab, INCMGA00012, AMP-224, and AMP-514, or the PD-L1 inhibitor is selected from atezolizumab, durvalumab, KN035, CK-301, AUNP12, CA-170, and BMS-986189; or The CTLA-4 inhibitor is selected from ipilimumab and tremelimumab. The combination of claim 1.

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