Phagocytosable particle for use in treatment or prophylaxis of cancer

Abstract

To provide improved immunotherapies for use in the treatment of cancers.SOLUTION: The invention provides a phagocytosable particle for use in the treatment or prophylaxis of cancer in a subject, the phagocytosable particle comprising a core and a neoantigenic construct tightly associated to the core, the neoantigenic construct comprising a neoepitope peptide having an amino acid sequence corresponding to an amino acid sequence of a part of a protein or peptide known or suspected to be expressed by a cancer cell in the subject, and the part of the protein or peptide having at least one somatic mutated amino acid. The invention also relates to injectable pharmaceutical compositions for use in the treatment or prophylaxis of cancer.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to phagocytosable particles comprising a neoantigenic construct tightly associated with a core for use in the treatment or prevention of cancer. The present invention also relates to an injectable pharmaceutical composition for use in the treatment or prevention of cancer. [Background technology]

[0002] There are various approaches to modulate the immune system of a subject to treat cancer, and such approaches are often referred to as "immunotherapy". Examples of immunotherapy include immune checkpoint inhibitors, adoptive cell transfer (ACT) therapy, and cancer vaccines.

[0003] There have been many successful examples of using immune checkpoint inhibitors to treat cancer, including the use of monoclonal antibodies that target binding interactions important for immune activation checkpoints. Immune checkpoint inhibitors have been used to treat a variety of cancers, including melanoma, lung cancer, bladder cancer, and gastrointestinal cancer.

[0004] Adoptive cell transfer (ACT) has also met with some success. For example, a study was reported by Karlsson et al., Ann Surg Oncol., 2010, 17(7):1747-57, in which patients with advanced colon cancer were treated with an adoptive immunotherapy protocol. The treatment was based on the isolation and in vitro expansion of autologous tumor-reactive lymphocytes isolated during surgery from the first lymph node that naturally drains the tumor (sentinel node). Sentinel node-acquired lymphocytes were collected, activated, expanded against autologous tumor extract, and returned to the patient as a blood transfusion. No toxic side effects or other adverse effects were observed. Complete or significant regression of disease occurred in four patients with liver and lung metastases, and 12 patients showed partial regression or stable disease.

[0005] A major limitation of ACT is the need to prepare sufficient amounts of anti-cancer T cells, such as tumor infiltrating lymphocytes (TILs), for administration to subjects. For example, current methods often require the use of invasive surgical procedures to remove the subject's cancer or cancer cells to obtain anti-cancer T cells. Furthermore, the obtained cells are often low in number and unresponsive due to immune suppressive mechanisms derived from cancer. This can lead to slow in vitro expansion, which means that it can take a long time to obtain sufficient amounts of anti-cancer T cells for use in therapy.

[0006] Engineered T cells have been developed to overcome some of the limitations of ACT. Engineered T cells can be obtained by genetically redirecting the specificity of T cells towards the patient's cancer by introduction of an antigen receptor or by introducing synthetic recognition structures called "chimeric antigen receptors" into T cells. Although engineered T cells have been successful in treating hematological cancers, the safety and selectivity of engineered T cells still need to be improved for the treatment of solid tumors.

[0007] An alternative approach to immune checkpoint inhibitors and ACT is to administer cancer antigens to subjects to induce anti-cancer immune responses. Compositions that induce anti-cancer immune responses are often called "cancer vaccines". Cancer vaccines typically contain cancer antigens, such as tumor-associated antigens (TAA) or tumor-specific antigens (TSA). TAAs are abnormally expressed by cancer cells, e.g., TAAs are proteins or peptides that can be expressed by both normal and cancer cells, but are expressed at significantly higher levels by cancer cells. TSAs are antigens that are expressed by cancer cells but not by normal cells. A notable example of a tumor-specific antigen is a neoantigen. Neoantigens are mutated proteins or peptides expressed by cancer cells but not by normal cells, and can be bound by molecules of the immune system, such as antibodies or T cell receptors (TCRs) on T cells. Regions of neoantigens that contain one or more cancer-specific amino acid mutations and are known or suspected to directly bind to molecules of the immune system are often referred to as neoepitopes. Therapeutics or vaccines that target TSAs, such as neoantigens, are expected to provide more effective and safe cancer treatments.

[0008] Despite the interest in immunotherapies for cancer treatment, their success to date has been limited due to challenges related to the safety and selectivity of immunotherapies as well as their ineffectiveness in modulating or inducing anti-cancer immune responses.

[0009] Thus, there remains a need for improved immunotherapies for use in the treatment of cancer that induce robust and targeted anti-cancer immune responses and that are suitable for use in clinical settings. Summary of the Invention

[0010] The present invention provides a phagocytosis particle for use in treating or preventing cancer in a subject, the phagocytosis particle comprising a core and a neoantigenic construct tightly associated with the core, the neoantigenic construct comprising a neoepitope peptide having an amino acid sequence corresponding to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by cancer cells in the subject, the portion of the protein or peptide having at least one somatically mutated amino acid.

[0011] The present invention also provides a method for treating or preventing cancer, comprising the step of administering to a subject a phagocytizable particle, the phagocytizable particle comprising a core and a neoantigenic construct tightly associated with the core, the neoantigenic construct comprising a neoepitope peptide having an amino acid sequence corresponding to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by cancer cells of the subject, the portion of the protein or peptide having at least one somatically mutated amino acid.

[0012] The present invention also provides a use of a phagocytosis particle for the manufacture of a medicament for treating or preventing cancer, the phagocytosis particle comprising a core and a neoantigenic construct tightly associated with the core, the neoantigenic construct comprising a neoepitope peptide having an amino acid sequence corresponding to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by a cancer cell of a subject, the portion of the protein or peptide having at least one somatically mutated amino acid.

[0013] The present invention also provides an injectable pharmaceutical composition comprising a phagocytizable particle, the phagocytizable particle comprising a core and a neoantigenic construct tightly associated with the core, the neoantigenic construct comprising a neoepitope peptide having an amino acid sequence corresponding to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by a cancer cell of a subject, the portion of the protein or peptide having at least one somatically mutated amino acid.

[0014] The invention further provides a phagocytizable particle of the invention for use in the treatment or prevention of cancer in a subject, or an injectable pharmaceutical composition of the invention for use in the treatment or prevention of cancer in a subject, or a method for the treatment or prevention of cancer in a subject of the invention, wherein the treatment or prevention of cancer comprises: administering one or more subsequent doses of the phagocytizable particles or injectable pharmaceutical composition to the subject. wherein the subject has previously been administered a dose of the phagocytizable particle or injectable pharmaceutical composition sufficient to elicit an immune response against cancer cells in the subject.

[0015] The invention further provides a phagocytizable particle of the invention for use in the treatment or prevention of cancer in a subject, or an injectable pharmaceutical composition of the invention for use in the treatment or prevention of cancer in a subject, or a method for the treatment or prevention of cancer in a subject of the invention, wherein the treatment or prevention of cancer comprises: (a) administering phagocytosis-capable particles to the subject, and then collecting APCs and anti-cancer T cells from the subject; (b) expanding anti-cancer T cells obtained from the subject; (c) administering to the subject a therapeutic dose of the expanded anti-cancer T cells.

[0016] The inventors have found that after administration to a subject, the phagocytizable particles described herein are taken up by antigen-presenting cells (APCs). The relevant neoantigenic constructs are then presented on the surface of the APCs, resulting in the activation and proliferation of anti-cancer T cells in the subject. The use of phagocytizable particles results in a surprising number of neoantigenic constructs being taken up, followed by the presentation of a wide variety of neoepitopes on the surface of the APCs. In a mouse model, the inventors have shown that administration of phagocytizable particles containing two types of neoantigenic constructs to the inguinal lymph nodes by injection or subcutaneously results in a dose-dependent increase in anti-neoepitope antibodies in serum samples taken from the mice. The same mice were then injected with melanoma cancer cells (B16F10). Advantageously, the inventors have found that administration of the phagocytizable particles to mice prior to injection of cancer cells results in a dose-dependent prophylactic effect against tumor growth in the mice. Thus, the inventors have discovered that by administering to a subject the phagocytizable particles described herein, a robust anti-cancer immune response can be induced in the subject, and the phagocytizable particles of the present invention can be used as a prophylactic vaccination against cancer to reduce cancer growth.

[0017] Furthermore, the present inventors also demonstrated in a mouse xenograft model of colorectal cancer that administration of a phagocytizable particle composition containing six neoantigenic constructs before and after transplantation of colon cancer cells (MC-38 cell line) resulted in a robust anti-cancer immune response in mice that inhibited tumor growth. As described above, the present inventors demonstrated the therapeutic and prophylactic potential of the phagocytizable particles of the present invention in two mouse models of cancer.

[0018] The inventors have also found that the core of the phagocytizable particles (e.g., polymer particles) described herein serves as a very effective carrier for the neoantigenic construct. Without wishing to be bound by any particular theory, it is believed that the phagocytizable particles defined herein are particularly effective because the entire phagocytizable particle, including the core and the tightly associated neoantigenic construct, is taken up into the phagosome by phagocytosis of the APC. The neoantigenic construct is then cleaved from the core of the particle and processed in the phagosome. Fragments of the neoantigenic construct are then presented on the surface of the APC via the major histocompatibility (MHC) class II pathway and presented on the cell surface by MHC class II molecules. It is believed that this is not the exclusive process by which neoepitopes are presented on the surface of the APC, and that some fragments of the neoantigenic construct may also be presented on the surface of the APC via the major histocompatibility (MHC) class I pathway and may be presented on the cell surface by MHC class I molecules in a process known as cross-presentation. Thus, fragments of the neoantigenic construct are expected to be presented on APCs primarily via the MHC class II pathway, but some are expected to be presented via the MHC class I pathway, and the present invention utilizes both pathways to a greater or lesser extent.

[0019] When antigens are presented by MHC class II molecules, they usually activate helper T cells (also known as CD4+ T cells), which direct the immune response primarily by secreting cytokines, induce class switching in B cells to help them make antibodies, and stimulate the activation and proliferation of other T cell types, especially cytotoxic T cells (e.g., CD8+ T cells) and memory T cells (e.g., CD8+ memory T cells). In addition, CD4+ T cells can directly kill other cell types (Borst et al., Nat Rev Immunol, 2018, 18(10), 635-647). This means that by administering phagocytizable particles as defined herein to a subject, it is possible to activate multiple types of immune cells, which results in an anti-cancer immune response that is slow-onset (and therefore has few side effects), has a long-lasting effect, and can target cancer in many different ways by harnessing the entire immune system (rather than just activating CD8+ T cells, which can only attack tumor cells directly). This is in contrast to what would be expected to happen if the antigen (e.g., neoantigen) is provided as a free peptide or a nucleotide construct expressing the peptide. Such antigens are expected to be incorporated into the cytosol of APCs, resulting in neoantigens being presented on the cell surface by MHC class I molecules exclusively via the MHC class I pathway. This leads to activation of primarily CD8+ T cells. Furthermore, after inducing an anti-cancer immune response, memory T cells derived from anti-cancer T helper cells persist in the blood circulation and can mount a rapid and effective secondary immune response while neoepitope-expressing cancer cells persist in the body or if the same cancer recurs.

[0020] The inventors have also found that the phagocytizable particles described herein can be efficiently purified and sterilized, e.g., contaminants such as pathogens (e.g., bacteria, fungi and viruses), endotoxins and other antigenic contaminants can be removed from the phagocytizable particles prior to administration to a subject. This is particularly advantageous because removing contaminants from the phagocytizable particles described herein reduces non-specific immune responses in the subject following administration, thereby improving the safety and efficacy of the phagocytizable particles.

[0021] The inventors have also realised that the phagocytizable particles are well tolerated by subjects following administration, due in part to the inert properties of the core and the high sterility of the phagocytizable particles. [Brief description of the drawings]

[0022] [Figure 1] FIG. 1 shows the effect of phagocytic particle size on T cell activation. A proliferation assay (by thymidine incorporation) was used to assess the number of splenocytes obtained from ovalbumin-sensitized mice. A comparison of ovalbumin bound to different sized phagocytizable particles with diameters of 5.6 μm, 1 μm, and 0.2 μm is shown. P values ​​were determined using Student's T test and are noted when p<0.05. Staples indicate SD. [Figure 2-1] FIG. 2 shows T cell proliferation by stimulation with neoepitopes NA1-9. FIG. 2A shows cell numbers over time in culture. FIG. 2B shows % of CD4+ T cells / total T cells. FIG. 2C shows T-bet expression in CD4+ T cells. FIG. 2D shows expression of granzyme B and perforin in CD8+ T cells. [Figure 2-2] Figure 2-2 is a continuation of Figure 2. Figure 2C shows T-bet expression in CD4+ T cells. Figure 2D shows granzyme B and perforin expression in CD8+ T cells. [Diagram 3]Figure 3 shows T cell proliferation upon stimulation with neoantigenic constructs. The total number of T cells (small squares) and CD4+ T cells (large squares) and the percentage of proliferating CD4+ cells (circles) are shown. [Figure 4-1] FIG. 4 shows the proliferation of anti-cancer T cells upon stimulation with neoantigenic constructs. FIG. 4A shows the number of cells over time (days) in PBMC cultures. PBMC cultures contain various cell types including APCs and T cells. The top line in FIG. 4A (Pat2 personalized NA) shows the number of cells in PBMC cultures after incubation with phagocytizable particles containing a polystyrene core (MyOne™ Carboxylic Acid Dynabeads®) and a personalized neoantigenic construct (SEQ ID NO: 3) tightly associated to the core. The bottom two lines in FIG. 4A (Pat2 NA 1+3 and Pat2 NA 4+5) show the number of cells in two separate PBMC cultures after incubation with phagocytizable particles containing a polystyrene core (MyOne™ Carboxylic Acid Dynabeads®) and a predicted neoantigenic construct tightly associated to the core. FIG. 4B shows the % of CD4+ T cells / total T cells. [Figure 4-2] Figure 4-2 is a continuation of Figure 4. Figure 4C visualizes the analysis performed using the Barnes-Hut Stochastic Neighbor Embedding (BH-SNE) algorithm on CD4+ T cells, where all cells in the sample are clustered on a 2D map according to the similarity of expression intensity of a set of selected markers (CD28, CD57, T-bet, GATA-3, perforin, granzyme B (GZB), Ki-67, PD-1). [Diagram 5]Figure 5A shows confocal microscopy images of PBMCs with phagocytosed particles in cells. Three sizes of phagocytosed particles are shown (4.5 μm, 2.8 μm or 1 μm) after 18 hours of incubation of PBMCs with phagocytosed particles at 37°C. Figure 5B shows the cellular uptake of two sizes of phagocytosed particles (4.5 μm or 2.8 μm) after 18 hours of incubation at 37°C with PBMCs, as assessed by manual counting. Figure 5C shows the cellular uptake of three sizes of phagocytosed particles (4.5 μm, 2.8 μm or 1 μm) after 18 hours of incubation at 37°C in PBMCs, as assessed by volumetric counting (*p<0,05 **p<0,01 ***p<0,001, calculated using Student's T-test). [Figure 6-1] Figure 6A shows the relative increase in IFNγ production levels in PBMCs from a CMV-susceptible healthy donor (n=2) stimulated with phagocytizable particles of three sizes (4.5 μm, 2.8 μm, or 1 μm) compared to unstimulated cells, as assessed by the FluoroSpot assay of Example 3a(iv). Figure 6B shows the relative increase in IL22 production levels in PBMCs from a CMV-susceptible healthy donor (n=1) stimulated with phagocytizable particles of three sizes (4.5 μm, 2.8 μm, or 1 μm) compared to unstimulated cells, as assessed by the FluoroSpot assay of Example 3a(iv). Figure 6C shows the relative increase in IL17 production levels in PBMCs from a CMV-susceptible healthy donor (n=1) stimulated with phagocytizable particles of three sizes (4.5 μm, 2.8 μm, or 1 μm) compared to unstimulated cells, as assessed by the FluoroSpot assay of Example 3a(iv). [Figure 6-2]Figure 6-2 is a continuation of Figure 6. Figure 6D shows the relative increase in dual cytokine production of IFNγ and IL-17 in PBMCs from a CMV-susceptible healthy donor (n=1) stimulated with phagocytizable particles of three sizes (4.5 μm, 2.8 μm, or 1 μm) compared to unstimulated cells, as assessed by the FluoroSpot assay of Example 3a(iv). Figure 6E shows the relative increase in dual cytokine production of IL22 and IL17 in PBMCs from a CMV-susceptible healthy donor (n=1) stimulated with phagocytizable particles of three sizes (4.5 μm, 2.8 μm, or 1 μm) compared to unstimulated cells, as assessed by the FluoroSpot assay of Example 3a(iv). [Figure 7A]Figures 7A, 7B, 7C and 7D show the percentage of anti-neoepitope antibodies in serum samples taken from mice after administration of low or high doses of two types of phagocytizable particles into the inguinal lymph node or subcutaneously (n=3 for each dose and route of administration). The two types of phagocytizable particles were 1) polystyrene particles tightly associated with neoantigenic construct M272120 (SEQ ID NO: 1) and 2) polystyrene particles tightly associated with neoantigenic construct M304748 (SEQ ID NO: 2). Figures 7A and 7B show the percentage of anti-neoepitope antibodies against neoantigenic construct M272120 (SEQ ID NO: 1) (Figure 7A) or against neoantigenic construct M304748 (SEQ ID NO: 2) (Figure 7B) in serum taken from mice 22 days after the first administration of phagocytizable particles. 7C and 7D also show the percentage of anti-neoepitope antibodies against neoantigenic construct M272120 (SEQ ID NO: 1) (FIG. 7C) or against neoantigenic construct M304748 (SEQ ID NO: 2) (FIG. 7D) in serum collected from mice 23 days after the second administration of phagocytizable particles, approximately one month after the first administration of phagocytizable particles. As a control, serum samples collected from naive mice (n=3, no administration of phagocytizable particles) were also analyzed. Mice that received one or two doses of high doses of phagocytizable particles (administered intrainguinal or subcutaneously) had a higher percentage of anti-neoepitope antibodies in serum samples compared to mice that received one or two doses of low doses of phagocytizable particles via the same route, and compared to mice that did not receive any dose of phagocytizable particles (i.e., naive mice). [Figure 7B] FIG. 7B shows the percentage of anti-neoepitope antibodies against the neoantigenic construct M304748 (SEQ ID NO: 2) in serum collected from mice 22 days after the first administration of phagocytizable particles (FIG. 7B). [Figure 7C]Figure 7C shows the percentage of anti-neoepitope antibodies against the neoantigenic construct M272120 (SEQ ID NO: 1) in serum collected from mice 23 days after the second administration of phagocytosis particles, approximately one month after the first administration of phagocytosis particles (Figure 7C). As a control, serum samples collected from naive mice (n=3, no administration of phagocytosis particles) were also analyzed. [Figure 7D] Figure 7D shows the percentage of anti-neoepitope antibodies against the neoantigenic construct M304748 (SEQ ID NO: 2) in serum collected from mice 23 days after the second administration of phagocytosis particles, approximately one month after the first administration of phagocytosis particles. As a control, serum samples collected from naive mice (n=3, no administration of phagocytosis particles) were also analyzed. [Figure 8] FIG. 8 shows the time course of tumor volume (days, D) after injection of melanoma cancer cell line B16F10 into mice previously treated with two doses of phagocytizable particles (polystyrene particles tightly associated with M272120 and polystyrene particles tightly associated with M304748). The time course of tumor volume is shown for mice (n=3) previously treated with the following doses of phagocytizable particles (2 doses of low dose phagocytizable particles administered to the inguinal lymph node (squares ■), 2 doses of high dose phagocytizable particles injected into the inguinal lymph node (triangles ▼), 2 doses of low dose phagocytizable particles injected subcutaneously (triangles ▲), 2 doses of high dose phagocytizable particles injected subcutaneously (diamonds ◆)). Administration of phagocytizable particles shows a dose-dependent preventive effect on tumor volume. [Figure 9] Figure 9 shows tumor volumes in mice (n=5) after one and two doses of phagocytosis particle composition containing six different groups of phagocytosis particles, each group containing a core bound to a different MC38 neoantigen construct (SEQ ID NOs: 13-18). The first dose was administered on day -5 (time point A) and the second dose was administered on day 13 (time point C). Mice were implanted with MC38 tumor cells on day 0 (time point B). Tumor progression was compared to a non-vaccinated group (negative control, n=5). Differences in tumor volumes at the end of the experiment were calculated using Student's t-test. ***p<0.001. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0023] Neoantigenic constructs and neoepitope peptides Phagocytizable particles for use in the present invention comprise a neoantigenic construct tightly associated with a core. The neoantigenic construct of the present invention comprises a neoepitope peptide.

[0024] A neoepitope peptide for use in the present invention is a peptide having an amino acid sequence that corresponds to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by cancer cells in a subject, the portion of the protein or peptide having at least one somatically mutated amino acid. The "somatically mutated amino acid" of the neoepitope peptide refers to a peptide that has an amino acid sequence that corresponds to the amino acid sequence of a portion of the protein or peptide known or suspected to be expressed by cancer cells in a subject, the portion of the protein or peptide having at least one somatically mutated amino acid. The neoepitope peptide may be a somatically mutated amino acid that is different from or not present in said portion of a protein or peptide corresponding to the neoepitope peptide amino acid sequence when expressed by a cancer cell (e.g., a cell). For example, a "somatically mutated amino acid" of a neoepitope peptide may be a deletion (i.e., an amino acid that is deleted), an addition (i.e., an amino acid that is added), or a substitution (i.e., an amino acid that is replaced with a different amino acid). Such somatically mutated amino acids may also be referred to as "cancer-specific somatically mutated amino acids" because the somatically mutated amino acids are present in cancer cells but not in normal cells (e.g., somatic cells). Preferably, the neoepitope peptide is a substitution (i.e., one or more amino acids are replaced with a different amino acid).

[0025] Mutant amino acids in proteins or peptides expressed by a cell can result from infidelity of DNA replication that occurs with each cell division, which causes substitution, deletion or insertion of nucleotides into the DNA of the cell. Nucleotide substitutions can result in different amino acids being coded for by the somatic non-mutated nucleic acid sequence, resulting in different amino acids in the protein / peptide than in the protein / peptide of a normal non-cancerous cell (e.g., somatic cell). Nucleotide insertions and / or deletions can result in reading frame errors (i.e., "frameshift mutations"), which can result in new amino acid sequences at the protein level (i.e., the insertion or deletion of nucleotides changes the reading frame of the DNA, which, after mutation, changes most or all of the amino acids coded for by the DNA compared to a normal cell (e.g., somatic cell). Additionally or alternatively, insertions and / or deletions can result in the introduction of stop codons, which can result in truncated proteins at the protein level. Nucleotide substitutions can individually change codons, which can result in amino acid substitutions and / or the introduction of stop codons at the protein level, resulting in truncated proteins at the protein level.

[0026] A neoepitope peptide for use in the present invention is a peptide having an amino acid sequence that corresponds to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by cancer cells in a subject, where the protein or peptide portion has at least one somatically mutated amino acid (e.g., 1, 2, 3, 4 or 5 or more somatically mutated amino acids).

[0027] A mutant protein or peptide known or suspected to be expressed by cancer cells in a subject may also be referred to as a "cancer-specific mutant protein or peptide" because the mutated protein or peptide is known or suspected to be expressed in cancer cells but not in normal cells (e.g., somatic cells).

[0028] Cancer-specific mutant proteins or peptides and their amino acid sequences that may correspond to the amino acid sequence of the neoepitope peptide can be identified using various techniques. For example, cancer-specific mutant proteins or peptides and their amino acid sequences, including their cancer-specific somatic mutant amino acids, can be identified using the COSMIC database ( Forbes et al., Nucleic Acids Res, 45(D1), D777-D783, this database Somatically mutated amino acid sequences identified from the COSMIC database or similar databases are referred to herein as "predicted neoepitope peptides." A neoantigenic construct comprised of one or more "predicted neoepitope peptides" is referred to herein as a "predicted neoantigenic construct."

[0029] In an alternative or additional approach to identifying cancer-specific mutant proteins or peptides, the amino acid sequence, i.e., the neoepitope peptide amino acid sequence, is identified from the cancer of interest. The reference sequences may correspond to the genome, exome transcriptome and / or proteome of cancer cells obtained from the subject, so that mutations in the cancer cells are predicted. This can be done, for example, by comparison of the proteome, genome, exome or transcriptome derived data with a reference nucleotide or amino acid sequence. Suitable reference sequences can be obtained from the genome, exome or transcriptome of non-cancerous cells (e.g., somatic cells) obtained from the subject, or from publicly available nucleotide or protein databases such as the UniProt database (https: / / www.uniprot.org / ) and the EBI expression atlas (https: / / www.ebi.ac.uk / gxa / home), which provide information on proteins and peptides expressed in tissues and cancer cell lines. In an alternative or additional approach, the cancer-specific mutant proteins or peptides and their amino acid sequences to which the amino acid sequence of the neoepitope peptide may correspond may have been previously identified by analysis of the genome, exome, transcriptome and / or proteome of the subject's tumor. Suitable techniques for determining the genome, exome or transcriptome of cancer or normal cells are known in the art and include, for example, Sanger sequencing and next generation sequencing. Suitable techniques for obtaining proteome data include multiple reaction monitoring (MRM) mass spectrometry. The somatically mutated amino acid sequences identified from genome, exome, transcriptome or proteome data obtained from a subject are referred to herein as "individualized neoepitope peptides." A neoantigenic construct consisting of one or more "individualized neoepitope peptides" is referred to herein as a "individualized neoantigenic construct."

[0030] A neoepitope peptide for use in the present invention may have one or more somatically mutated amino acids. For example, it may have one somatically mutated amino acid, or it may have two or more somatically mutated amino acids, i.e., from two to all amino acids in a portion of a protein or peptide may be mutated. For example, a neoepitope peptide of the present invention may have 1 to 10 (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10) somatically mutated amino acids, more preferably 1 to 8 (e.g., 1, 2, 3, 4, 5, 6, 7 or 8) mutated amino acids. Or, for example, it may have 1 to 6 (e.g., 1, 2, 3, 4, 5 or 6) mutated amino acids, or for example, it may have 1 to 5 (e.g., 1, 2, 3, 4 or 5) somatically mutated amino acids, or for example, it may have 1 to 4 (e.g., 1, 2, 3 or 4) somatically mutated amino acids. In a preferred embodiment of the present invention, the neoepitope peptide of the present invention has 1, 2 or 3 somatically mutated amino acids. In a preferred embodiment, the neoepitope peptide of the present invention has 1 or 2 somatically mutated amino acids. Even more preferably, the neoepitope peptide of the present invention has 1 somatically mutated amino acid.

[0031] In a preferred embodiment, the neoepitope peptide of the present invention has somatically mutated amino acids in most or all of its parts. Even more preferably, the neoepitope peptide of the present invention has somatically mutated amino acids in most or all of its parts. Such neoepitope peptides having somatically mutated amino acids in most or all of its parts may correspond to a portion of a protein or peptide that arises by a frameshift mutation in the DNA of a cell.

[0032] The one or more amino acid mutations of the neoepitope peptide for use in the present invention may be located at any amino acid position within the amino acid sequence of the neoepitope peptide. In a preferred embodiment, at least one of the somatically mutated amino acids (or one somatically mutated amino acid in an embodiment in which there is only one somatically mutated amino acid in the neoepitope peptide) is located in the central portion of the neoepitope peptide. For example, the neoepitope peptide amino acid sequence If the string is at least 3 amino acids long (e.g., at least 5 amino acids long or at least 7 amino acids long), the central portion of the neoepitope peptide is one central amino acid of the sequence if the neoepitope peptide has an odd number of amino acids in its sequence, or two central amino acids if the neoepitope peptide has an even number of amino acids in its sequence. For example, if the neoepitope peptide amino acid sequence is at least 9 amino acids long, the central portion of the neoepitope peptide is three central amino acids (and preferably one central amino acid) of the sequence if the neoepitope peptide has an odd number of amino acids in its sequence, or four central amino acids (and preferably two central amino acids) if the neoepitope peptide has an even number of amino acids in its sequence. For example, when the neoepitope peptide amino acid sequence is at least 11 amino acids long, the central portion of the neoepitope peptide is the central 5 amino acids (and preferably one central amino acid) of the sequence when the neoepitope peptide has an odd number of amino acids in its sequence, and the central 6 amino acids when the neoepitope peptide has an even number of amino acids in its sequence. More preferably, when the neoepitope peptide amino acid sequence is at least 11 amino acids long, the central portion of the neoepitope peptide is the central 3 amino acids (and preferably one central amino acid) of the sequence when the neoepitope peptide has an odd number of amino acids in its sequence, and the central 4 amino acids (and preferably two central amino acids) when the neoepitope peptide has an even number of amino acids in its sequence.

[0033] In a preferred embodiment, at least one of the somatically mutated amino acids of the neoepitope peptide (or one somatically mutated amino acid in an embodiment in which only one somatically mutated amino acid is present in the neoepitope peptide) is located in a central position of the neoepitope peptide if the neoepitope peptide has an odd number of amino acids in its sequence, or in one of the two central most positions of the amino acid sequence if the neoepitope peptide has an even number of amino acids in its sequence.

[0034] In certain embodiments of the invention, most or all of the amino acids of the neoepitope peptide are somatically mutated amino acids. In such embodiments, the somatically mutated amino acids in a protein or peptide expressed by a cancer cell may have arisen due to an error in the reading frame of the coding DNA (i.e., due to a frameshift mutation), such that all or most of the amino acids in a portion of the protein or peptide differ from the portion of the protein or peptide expressed in a normal, non-cancerous cell.

[0035] Neoepitope peptides of the present invention can have an amino acid sequence of 3 to 200 amino acids in length (e.g., 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 50, 75, 100, 125, 150, 175 or 200 amino acids in length). Preferably, the neoepitope peptides of the present invention are 3 to 50 amino acids in length (e.g., 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, or 50 amino acids in length), more preferably 3 to 30 amino acids in length (e.g., 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, or 50 amino acids in length). 7, 28, 29 or 30 amino acids in length), more preferably 3 to 25 amino acids (e.g. 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 amino acids in length), more preferably 5 to 25 amino acids (e.g. 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 amino acids in length), more preferably 8 to 25 amino acids (e.g. 8, 9, 10, The neoepitope peptide of the present invention may have an amino acid sequence of 3 to 25 amino acids in length, 5 to 25 amino acids in length, 10 to 25 amino acids in length, 11 to 25 amino acids in length, 19 to 25 amino acids in length, 20 to 25 amino acids in length, 21 to 25 amino acids in length, 22 to 25 amino acids in length, 23 to 24 amino acids in length, 24 to 25 amino acids in length, 25 to 25 amino acids in length, 26 to 25 amino acids in length, 27 to 25 amino acids in length, 28 to 25 amino acids in length, 29 to 25 amino acids in length, 30 to 35 amino acids in length, 31 to 35 amino acids in length, 32 to 35 amino acids in length, 33 to 35 amino acids in length, 34 to 35 amino acids in length, 35 to 35 amino acids in length, 36 to 35 amino acids in length, 37 to 35 amino acids in length, 38 to 35 amino acids in length, 39 to 35 amino acids in length, 40 to 45 amino acids in length, 41 to 45 amino acids in length, 42 to 45 amino acids in length, 43 to 45 amino acids in length, 44 to 45 amino acids in length, 45 to 45 amino acids in length, 46 to 45 amino acids in length, 47 to 45 amino acids in length, 48 to 45 amino acids in length, 49 to 50 amino acids in length, 50 ... In another preferred embodiment, the neoepitope peptide of the present invention has a length of 3 to 23 amino acids, 5 to 23 amino acids, 10 to 23 amino acids, 11 to 23 amino acids, 12 to 23 amino acids, 13 to 23 amino acids, 15 to 23 amino acids, 17 to 23 amino acids, 19 to 23 amino acids, 20 to 23 amino acids, or 21 to 23 amino acids. In another preferred embodiment, the neoepitope peptide of the present invention has a length of 3 to 21 amino acids, 5 to 21 amino acids, 10 to 21 amino acids, 11 to 21 amino acids, 12 to 21 amino acids, 13 to 21 amino acids, 15 to 21 amino acids, 17 to 21 amino acids, or 19 to 21 amino acids. In another preferred embodiment, the neoepitope peptide of the present invention is 3 to 19 amino acids long, 5 to 19 amino acids long, 10 to 19 amino acids long, 11 to 19 amino acids long, 12 to 21 amino acids long, 13 to 21 amino acids long, 15 to 21 amino acids long, or 17 to 19 amino acids long. In another preferred embodiment, the neoepitope peptide of the present invention has a length of 3 to 17 amino acids long, 5 to 17 amino acids long, 10 to 17 amino acids long, 11 to 17 amino acids long, 12 to 17 amino acids long, 13 to 17 amino acids long, or 15 to 17 amino acids long. In another preferred embodiment, the neoepitope peptide of the present invention has a length of 3 to 15 amino acids long, 3 to 15 amino acids long, 10 to 15 amino acids long, 11 to 15 amino acids long, 12 to 15 amino acids long, or 13 to 15 amino acids long.In another preferred embodiment, the neoepitope peptide of the present invention is 3 to 19 amino acids in length, 5 to 17 amino acids in length, 5 to 15 amino acids in length, 5 to 13 amino acids in length, or 5 to 10 amino acids in length. In another preferred embodiment, the neoepitope peptide of the present invention is 3 to 19 amino acids in length, 5 to 17 amino acids in length, 3 to 15 amino acids in length, 3 to 10 amino acids in length, or 5 to 10 amino acids in length. In another preferred embodiment, the neoepitope peptide of the present invention is 3 to 19 amino acids in length, 3 to 17 amino acids in length, 3 to 13 amino acids in length, 3 to 10 amino acids in length, or 3 to 7 amino acids in length.

[0036] In a preferred embodiment of the invention, the neoepitope peptide is 10 to 25 amino acids, 10 to 23 amino acids, 10 to 21 amino acids, 10 to 19 amino acids, 10 to 17 amino acids, 10 to 15 amino acids and includes 1, 2, 3, 4, or 5, or all somatically mutated amino acids. Preferably, the neoepitope peptide is 10 to 25 amino acids long, 10 to 23 amino acids long, 10 to 21 amino acids long, 10 to 19 amino acids long, 10 to 17 amino acids long, or 10 to 15 amino acids long and includes 1, 2, or 3 somatically mutated amino acids or all mutated amino acids. More preferably, the neoepitope peptide is 10 to 25 amino acids long, 10 to 23 amino acids long, 10 to 21 amino acids long, 10 to 19 amino acids long, 10 to 17 amino acids long, or 10 to 15 amino acids long and the neoepitope includes 1 or 2 somatically mutated amino acids or all mutated amino acids. Even more preferably, the neoepitope peptide is 10 to 25 amino acids long, 10 to 23 amino acids long, 10 to 21 amino acids long, 10 to 19 amino acids long, 10 to 17 amino acids long, 10 to 15 amino acids long and includes one somatically mutated amino acid or all mutated amino acids.Even more preferably, the neoepitope peptide is 10 to 25 amino acids long, 10 to 23 amino acids long, 10 to 21 amino acids long, 10 to 19 amino acids long, 10 to 17 amino acids long, 10 to 15 amino acids long and includes one somatically mutated amino acid.

[0037] In another preferred embodiment of the invention, the neoepitope peptide is 3 to 25 amino acids long, 3 to 17 amino acids long, 3 to 15 amino acids long, 3 to 10 amino acids long, or 5 to 10 amino acids long and comprises 1, 2, 3, 4, or 5, or all somatically mutated amino acids. Preferably, the neoepitope peptide is 3 to 25 amino acids long, 3 to 17 amino acids long, 3 to 15 amino acids long, 3 to 10 amino acids long, or 5 to 10 amino acids long and comprises 1, 2, or 3, or all somatically mutated amino acids. More preferably, the neoepitope peptide is 3 to 25 amino acids long, 3 to 17 amino acids long, 3 to 15 amino acids long, 3 to 10 amino acids long, or 5 to 10 amino acids long and comprises 1, 2, or 3, or all somatically mutated amino acids. Even more preferably, the neoepitope peptide is 3 to 25 amino acids long, 3 to 17 amino acids long, 3 to 15 amino acids long, 3 to 10 amino acids long, or 5 to 10 amino acids long and includes one or all somatically mutated amino acids.Even more preferably, the neoepitope peptide is 3 to 25 amino acids long, 3 to 17 amino acids long, 3 to 15 amino acids long, 3 to 10 amino acids long, or 5 to 10 amino acids long and includes one or all somatically mutated amino acids.

[0038] The inventors have advantageously found that the neoepitopes of the present invention, in particular those having an amino acid sequence that corresponds to the amino acid sequence of a portion of a cancer-specific protein or peptide that is 3 to 25 amino acids in length and that includes one or more somatically mutated amino acids, are particularly effective in eliciting an anti-cancer immune response in a subject, but do not elicit an autoimmune or non-cancer-specific immune response in a subject.

[0039] The neoantigenic construct may comprise one neoepitope peptide or may comprise two or more neoepitope peptides. For example, the neoantigenic construct may comprise from 1 to 50 neoepitope peptides (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50 neoepitope peptides). Preferably, the neoantigenic construct comprises 1 to 20 neoepitope peptides (e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 neoepitope peptides), more preferably 1 to 15 neoepitope peptides (e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15 neoepitope peptides), more preferably It is particularly preferred that the neo-antigenic construct comprises 1 to 5 neo-epitope peptides (e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10), more preferably 1 to 8 neo-epitope peptides (e.g. 1, 2, 3, 4, 5, 6, 7 or 8), more preferably 1 to 6 neo-epitope peptides (e.g. 1, 2, 3, 4, 5 or 6), even more preferably 1 to 5 neo-epitope peptides (e.g. 1, 2, 3, 4 or 5). It is particularly preferred that the neo-antigenic construct comprises 1 to 5 neo-epitope peptides (e.g. 1, 2, 3 or 4), even more preferably 3 to 5 neo-epitope peptides, e.g. 3, 4 or 5 neo-epitope peptides.

[0040] In one embodiment of the invention, the neoantigenic construct comprises two or more neoepitope peptides. For example, the neoantigenic construct may comprise between 2 and 50 neoepitope peptides (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50 neoepitope peptides). Preferably, the neoantigenic construct comprises between 2 and 20 neoepitope peptides. More preferably, the neoantigenic construct may comprise 2 to 15 neoepitope peptides (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 neoepitope peptides), more ...0 neoepitope peptides (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 neoepitope peptides). It is particularly preferred that the neo-antigenic construct comprises 2 to 5 neo-epitope peptides (e.g., 2, 3, 4, 5, 6, 7, 8, 9 or 10), more preferably 2 to 8 neo-epitope peptides (e.g., 2, 3, 4, 5, 6, 7 or 8), more preferably 2 to 6 neo-epitope peptides (e.g., 2, 3, 4, 5 or 6), even more preferably 2 to 5 neo-epitope peptides (e.g., 2, 3, 4 or 5). It is particularly preferred that the neo-antigenic construct comprises 2 to 5 neo-epitope peptides (e.g., 2, 3, 4 or 5), even more preferably 3 to 5 neo-epitope peptides, e.g., 3, 4 or 5 neo-epitope peptides.

[0041] In another embodiment of the invention, the neoantigenic construct comprises three or more neoepitope peptides. For example, the neoantigenic construct can comprise between 3 and 50 neoepitope peptides (e.g., 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50 neoepitope peptides). Preferably, the neoantigenic construct may comprise 3 to 20 neoepitope peptides (e.g., 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 neoepitope peptides), more preferably, the neoantigenic construct comprises 3 to 15 neoepitope peptides (e.g., 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 neoepitope peptides). It is particularly preferred that the neoantigenic construct comprises 3 to 5 neoepitope peptides (e.g., 3, 4 or 5), more preferably 3 to 10 neoepitope peptides (e.g., 3, 4, 5, 6, 7, 8, 9 or 10), more preferably 3 to 8 neoepitope peptides (e.g., 3, 4, 5, 6, 7 or 8), more preferably 3 to 6 neoepitope peptides (e.g., 3, 4, 5 or 6), even more preferably 3 to 5 neoepitope peptides (e.g., 3, 4 or 5).

[0042] In further embodiments of the invention, the neoantigenic construct comprises four or more neoepitope peptides (e.g., four neoepitope peptides), five or more neoepitope peptides (e.g., five neoepitope peptides), or six or more neoepitope peptides (e.g., six neoepitope peptides).

[0043] For the avoidance of doubt, in embodiments where a neoantigenic construct may comprise more than one neoepitope peptide (e.g., one or more neoepitope peptides, two or more neoepitope peptides, or three or more neoepitope peptides), each neoepitope peptide is a neoepitope peptide as described herein for use in the present invention (i.e., each neoepitope peptide of a neoantigenic construct has an amino acid sequence that corresponds to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by a cancer cell of a subject, the portion of the protein or peptide having at least one somatically mutated amino acid). In such embodiments, each neoepitope peptide may independently have any of the properties and / or characteristics of the neoepitope peptides described herein.

[0044] The neoantigenic construct may comprise two or more neoepitope peptides (e.g., one or more neoepitopes). In embodiments that may include a neoepitope peptide, two or more neoepitope peptides, or three or more neoepitope peptides, each neoepitope peptide may have the same amino acid sequence, or the neoepitope peptides may have different amino acid sequences (i.e., some neoepitope peptides of a neoantigenic construct may have different amino acid sequences, or all of the neoepitope peptides of a neoantigenic construct may have different amino acid sequences).

[0045] In one preferred embodiment, where the neoantigenic construct may comprise two or more neoepitope peptides, some or all of the neoepitope peptides have different amino acid sequences, more preferably all of the neoepitope peptides have different amino acid sequences. In another embodiment, where the neoantigenic construct comprises two or more neoepitope peptides, each neoepitope peptide has the same amino acid sequence.

[0046] A neoantigenic construct may comprise two or more neoepitope peptides (e.g., one or more neoepitope peptides, two or more neoepitope peptides, or three or more neoepitope peptides), and in embodiments where some of the neoepitope peptides have different amino acid sequences or all of the neoepitope peptides have different amino acid sequences, the amino acid sequences may differ for the following reasons: differ in a protein or peptide known or suspected to be expressed by cancer cells in the subject; or The protein or peptide known or suspected to be expressed by cancer cells in the subject is the same, but the corresponding portion of the amino acid sequence of the neoepitope peptide of the protein or peptide known or suspected to be expressed by cancer cells in the subject is different.

[0047] When the proteins or peptides known or suspected to be expressed by cancer cells in a subject are the same, but the corresponding portions of the amino acid sequences of the neoepitope peptides of the proteins or peptides known or suspected to be expressed by cancer cells in a subject are different, these portions may differ for one or more of the following reasons: A portion having at least one identical somatically mutated amino acid is longer; the portion having at least one identical somatically mutated amino acid is shorter, and / or the portions have at least one identical somatically mutated amino acid, but the position of the mutated amino acid differs with respect to the C-terminus and N-terminus; Portions of the same protein or peptide differ by having at least one different somatically mutated amino acid (eg, the protein or peptide has a frameshift mutation and the different portions are different portions of the frameshift mutant sequence of the protein or peptide).

[0048] In a preferred embodiment, the amino acid sequences for each neoepitope peptide are different due to different proteins or peptides known or suspected to be expressed by cancer cells in the subject.

[0049] In a preferred embodiment, the amino acid sequences are different because the proteins or peptides known or suspected to be expressed by cancer cells in the subject are the same, but the corresponding portions of the amino acid sequences of the neoepitope peptides of the proteins or peptides known or suspected to be expressed by cancer cells in the subject are different because they are different portions of a frameshift mutant sequence of the protein or peptide.

[0050] The neoantigenic construct may comprise two or more neoepitope peptides (e.g., two or more neoepitopes). In embodiments of the invention that include a neoepitope peptide or more than two neoepitope peptides, the neoepitope peptides may be linked directly or via a spacer moiety.

[0051] In certain preferred embodiments, where the neoantigenic construct comprises two or more neoepitope peptides (e.g., one or more neoepitope peptides, two or more neoepitope peptides, or three or more neoepitope peptides), the neoepitope peptides are covalently linked.

[0052] A neoantigenic construct of the invention that comprises more than one neoepitope peptide (e.g., two or more neoepitope peptides, or three or more neoepitope peptides) may comprise directly linked neoepitope peptides and / or neoepitopes linked via spacer moieties. When more than one spacer moiety is present in a neoantigenic construct, the spacer moieties of the neoantigenic construct may all be the same or may be different.

[0053] In a preferred embodiment, in which the neoantigenic construct comprises two or more neoepitope peptides (e.g., one or more neoepitope peptides, two or more neoepitope peptides, or three or more neoepitope peptides), each of the neoepitope peptides is linked via a spacer moiety.

[0054] The spacer portion may be a short sequence of amino acids, for example 1 to 15 amino acids, preferably 1 to 10 amino acids, more preferably 1 to 5 amino acids (e.g. 1, 2, 3, 4 or 5 amino acids). The spacer portion may comprise a random combination of amino acids or may comprise a synthetic amino acid sequence, such as a polylysine, polyarginine, polyglycine, polyalanine or polyhistidine amino acid sequence. Preferably, the spacer portion comprises the motifs VVR and / or GGS.

[0055] The structure of the neoantigenic construct containing two linked neoepitope peptides may be as follows: -[first neoepitope peptide]-[spacer portion]-[second neoepitope peptide]

[0056] The structure of a neoantigenic construct containing three linked neoepitopes may be as follows: -[first neoepitope peptide]-[spacer portion]-[second neoepitope peptide]-[spacer portion]-[third neoepitope peptide]

[0057] The structure of a neoantigenic construct containing four linked neoepitopes may be as follows: -[first neoepitope peptide]-[spacer portion]-[second neoepitope peptide]-[spacer portion]-[third neoepitope peptide]-[spacer portion]-[fourth neoepitope peptide]

[0058] The structure of a neoantigenic construct containing five linked neoepitopes may be as follows: -[first neoepitope peptide]-[spacer portion]-[second neoepitope peptide]-[spacer portion]-[third neoepitope peptide]-[spacer portion]-[fourth neoepitope peptide]-[spacer portion]-[fifth neoepitope peptide]

[0059] The structure of the neoantigenic construct containing n linked neoepitope peptides may be as follows: -[first neoepitope peptide]-[spacer portion]-[second neoepitope peptide]-[spacer portion]-[third neoepitope peptide].....-[spacer portion]-[nth neoepitope peptide]

[0060] In the case where the neoantigenic construct comprises more than one neoepitope peptide, the neoantigenic construct may consist of the neoepitope peptide and, optionally, any spacer portion. Alternatively, in the case where the neoantigenic construct comprises more than one neoepitope peptide, the neoantigenic construct may comprise the neoepitope peptide and, optionally, any spacer portion and an additional amino acid sequence. Such an additional amino acid sequence may be, for example, a random combination of amino acids (particularly a random sequence with a high proportion of histidine and / or cysteine ​​residues) or a synthetic amino acid sequence such as a polylysine, polyarginine, polyglycine, polyalanine, polyhistidine or polycysteine ​​amino acid sequence (and preferably, polyhistidine or polycysteine). Such additional amino acid sequences can be used, for example, as linkers and / or spacers between the core of the phagocytizable particle and the neoepitope peptide of the neoantigenic construct tightly associated therewith, or can be used to tightly associate the neoantigenic construct with the phagocytizable particle. For example, metal chelates can bind with high strength to proteins and peptides containing histidine or cysteine. Thus, a core with metal chelates can be non-covalently attached to neoantigenic constructs that contain polyhistidine or polycysteine ​​synthetic amino acid sequences and / or random combinations of amino acids with a high percentage of histidine and / or cysteine ​​residues as additional amino acid sequences. The neoantigenic constructs described herein can also include an albumin binding domain (ABD).

[0061] In an embodiment of the invention, when the neoantigenic construct comprises one neoepitope peptide, the neoantigenic construct may consist of the neoepitope peptide. Alternatively, when the neoantigenic construct comprises one neoepitope peptide, the neoantigenic construct may comprise the neoepitope peptide and an additional amino acid sequence. Such an additional amino acid sequence may be, for example, a random combination of amino acids (particularly a random sequence with a high proportion of histidine and / or cysteine ​​residues) or a synthetic amino acid sequence such as a polylysine, polyarginine, polyglycine, polyalanine, polyhistidine or polycysteine ​​amino acid sequence (and preferably polyhistidine or polycysteine). Such an additional amino acid sequence may be used, for example, as a linker and / or spacer between the core of the phagocytizable particle and the neoepitope peptide of the neoantigenic construct tightly associated therewith, or may be used to tightly associate the neoantigenic construct to the phagocytizable particle. For example, metal chelates can bind with great strength to histidine- or cysteine-containing proteins and peptides. Thus, a core carrying a metal chelate can be non-covalently attached to a neoantigenic construct that contains a polyhistidine or polycysteine ​​synthetic amino acid sequence and / or a random combination of amino acids with a high percentage of histidine and / or cysteine ​​residues as additional amino acid sequences.

[0062] The length of a neoantigenic construct for use in the present invention will depend, for example, on the number of neoepitope peptides in the neoantigenic construct and the length of each neoepitope peptide in the neoantigenic construct, as well as the length of any spacer portion that may be present when more than one neoepitope peptide is present and the length of any additional amino acid sequences that may be present. In a preferred embodiment, the neoantigenic construct of the present invention is between 3 and 300 amino acids in length, preferably between 10 and 250 amino acids in length, more preferably between 10 and 200 amino acids in length, even more preferably between 10 and 250 amino acids in length. or 10 to 180 amino acids in length. For example, neoantigenic constructs of the invention may comprise an amino acid sequence that is 11 to 150 amino acids in length, 11 to 140 amino acids in length, 33 to 120 amino acids in length, 42 to 140 amino acids in length, 11 to 112 amino acids in length, 33 to 112 amino acids in length, 2,42 to 112 amino acids in length, 11 to 100 amino acids in length, 33 to 100 amino acids in length, 42 to 100 amino acids in length, 11 to 84 amino acids in length, 33 to 84 amino acids in length, 42 to 84 amino acids in length, 11 to 60 amino acids in length, 33 to 60 amino acids in length, or 42 to 60 amino acids in length, 11 to 50 amino acids in length, 33 to 50 amino acids in length, or 42 to 50 amino acids in length.

[0063] Neoantigenic constructs for use in the present invention can be prepared recombinantly (e.g., in E. coli, mammalian cells or insect cells), synthetically (e.g., using standard organic chemistry techniques such as solution or solid phase peptide synthesis), or can be prepared from polypeptides isolated from naturally occurring proteins or peptides derived from animal sources (e.g., human sources). Preferably, neoantigenic constructs for use in the present invention are prepared recombinantly (e.g., in E. coli, mammalian cells or insect cells). More preferably, neoantigenic constructs for use in the present invention are prepared recombinantly in E. coli.

[0064] Phagocytizable particles and phagocytizable particle cores The phagocytosis particles of the present invention are particles that can be phagocytosed by cells of the immune system. However, it should be understood that the phagocytosis particles of the present invention can be taken up by cells of the immune system through different routes (e.g., pinocytosis, clathrin-mediated endocytosis and non-clathrin-mediated endocytosis). Preferably, the phagocytosis particles can be phagocytosed by APCs, such as monocytes, dendritic cells, B cells or macrophages, or other cells that phagocytose or take up extracellular molecules such as antigens and present antigen-derived peptides on MHC class II and / or MHC class I molecules to CD4+T cells and / or CD8+T cells.

[0065] Antigens taken up by APCs via the phagocytic pathway are degraded heterogeneously, and then APCs present a greater variety of antigen-derived peptides. Without wishing to be bound by theory, it is believed that the phagocytizable particles for use in the present invention further improve the activation and proliferation of anti-cancer T cells by phagocytosing the neoantigenic constructs by APCs, resulting in a greater variety of neoantigenic construct-derived peptides being presented by APCs, thus resulting in further activation and proliferation of anti-cancer T cells.

[0066] For particles to be phagocytosed by cells of the immune system, such as APCs, the particles must be within a suitable size range to allow phagocytosis. For example, particles that are too small may not induce phagocytosis by a particular APC, and particles that are too large may not allow phagocytosis by a particular APC. Complete phagocytosis results in good antigen degradation by APCs and subsequent presentation to T cells via the MHC class II pathway. The optimal size has been studied by the inventors (see Examples 3a and 3b, and Figures 1, 5A-C, and 6A-E).

[0067] The phagocytizable particle of the present invention comprises a core and a neoantigenic construct tightly associated with the core. Thus, the size of the core must be within such a range that when the core is tightly associated with the neoantigenic construct, the core and its tightly associated neoantigenic construct are phagocytosable by cells of the immune system, particularly APCs. It is preferred that the size of the core is within such a range that when the core is tightly associated with the neoantigenic construct, the core and its tightly associated neoantigenic construct are small enough that two or more phagocytizable particles can enter the same APC by phagocytosis. Having two or more phagocytosed particles in an APC maximizes the presentation of neoepitopes on the cell surface via the MHC class II pathway. Furthermore, Moreover, this allows particles carrying different neoantigenic constructs (especially neoantigenic constructs containing different neoepitope peptides) to enter the APC, which means that the APC can simultaneously present different neoepitopes from several particles in different phagosomes.

[0068] Thus, in a preferred embodiment, the core has a maximum dimension of less than 6 μm, less than 5.6 μm, less than 4 μm, less than 3 μm, less than 2.5 μm, less than 2 μm, or less than 1.5 μm. More preferably, the core has a maximum dimension of less than 1.5 μm. In another preferred embodiment, the core may have a maximum dimension of more than 0.001 μm, more than 0.005 μm, more than 0.01 μm, more than 0.05 μm, more than 0.1 μm, more than 0.2 μm, or more than 0.5 μm. More preferably, the core has a maximum dimension of more than 0.5 μm.

[0069] In a particularly preferred embodiment, the core has a maximum dimension in the range of 0.1 to 6 μm, for example 0.1 to 5.6 μm, 0.2 to 5.6 μm, 0.5 to 5.6 μm, 0.1 to 4 μm, or 0.5 to 4 μm. More preferably, the core has a maximum dimension in the range of 0.1 to 3 μm, for example 0.5 to 3 μm, 0.2 to 2.5 μm, 0.5 to 2.5 μm, 0.2 to 2 μm, 0.5 to 2 μm, or 1 to 2 μm. Even more preferably, the core has a maximum dimension of about 1 μm, about 1.5 μm, or about 2 μm. In a highly preferred embodiment of the present invention, the core is about 1 μm.

[0070] The core of the phagocytizable particle of the present invention can be in the form of any three-dimensional shape, for example, any regular or irregular three-dimensional shape. Preferably, the phagocytizable particle is substantially spherical, in which case the dimensions of the phagocytizable particle refer to the diameter.

[0071] The core of the phagocytizable particle can include a polymer, glass, ceramic material (e.g., the core can be a polymeric, glass, or ceramic particle). The material of the core can be a biodegradable and / or biocompatible material (e.g., the particle can be a biodegradable and / or biocompatible particle).

[0072] Preferably, the core comprises a polymer (e.g., the core is a polymer particle). If the core comprises a polymer, it may be selected from the group consisting of synthetic aromatic polymers (such as polystyrene, e.g., the core is a polystyrene particle), synthetic non-aromatic polymers (such as polyethylene, polylactic acid, poly(lactic-co-glycolic acid) and polycaprolactone, e.g., the core is a polyethylene particle, a polylactic acid particle, a poly(lactic-co-glycolic acid) particle or a polycaprolactone particle), natural polymers (such as collagen, gelatin, proteins (e.g., virus-like particles), lipids or albumin, e.g., the core is a collagen particle, a gelatin particle or an albumin particle), polymeric carbohydrate molecules (such as polysaccharides, e.g., agarose, alginate, chitosan or zymosan, e.g., the core is an agarose particle, an alginate particle, a chitosan particle or a zymosan particle).

[0073] In a preferred embodiment, the core comprises polystyrene or polyethylene, more preferably polystyrene (eg, the core is a polystyrene particle). Such polymers are biocompatible.

[0074] The inventors have found that polystyrene particles are particularly useful cores for the phagocytosis particles of the present invention, because polystyrene particles are non-toxic and widely available commercially in a variety of sizes and in a variety of functionalizable forms.Furthermore, the inventors have found that phagocytosis particles that include a polystyrene core, such as polystyrene particles, can withstand rigorous sterilization procedures for preparing the particles for administration to a subject.Such sterilization procedures may include repeated washing with acid or alkaline solutions and / or exposure to high temperatures.

[0075] In one highly preferred embodiment of the invention, the core is a polystyrene particle having a maximum dimension of less than 6 μm, preferably about 1 μm to about 3 μm, more preferably about 1 μm. Phagocytizable particles comprising a polystyrene particle core having a dimension of about 1 μm to about 3 μm, especially about 1 μm, are efficiently phagocytosed by APCs and are also able to withstand rigorous sterilization procedures to remove antigenic contaminants such as pathogens (e.g., bacteria, fungi and viruses) and pyrogens (e.g., endotoxins) that may be associated with the core or neoantigenic constructs.

[0076] In one embodiment of the invention, the core has magnetic properties. For example, the core may have paramagnetic or superparamagnetic properties. Preferably, the core has superparamagnetic properties.

[0077] An example of a superparamagnetic core suitable for use in the present invention is Dynabeads™ (Invitrogen). Dynabeads™ are available in a variety of functionalizable forms, such as Dynabeads M-270 Carboxylic Acid, Dynabeads M-270 Amine and Dynabeads MyOne Carboxylic Acid. Dynabeads™ are monosized superparamagnetic particles, composed of highly cross-linked polystyrene with uniformly distributed magnetic material. The magnetic material may be iron oxide. Other examples of magnetic cores, particularly superparamagnetic cores, include Encapsulated Carboxylated Estapo® SuperParamagnetic Microspheres (Merck-Chemie SAS) and Sera-Mag SpeedBeads (hydrophilic) carboxylate modified magnetic particles (GE Healthcare UK Limited). Encapsulated Carboxylated Estapor® SuperParamagnetic Microspheres are made of a core-shell structure that encapsulates an iron oxide core.

[0078] The phagocyte-able particles of the present invention comprise a neoantigenic construct tightly associated with a core. The neoantigenic construct can be tightly associated with the core using a variety of means. For example, the neoantigenic construct can be attached to the core by a covalent bond, e.g., an amide bond between an amine or carboxylic acid group of the neoantigenic construct and a carboxylic acid or amine group on the surface of the core. Alternatively, the neoantigenic construct can be linked to the core via a metal chelate. For example, a core linked with a metal chelating ligand such as iminodiacetic acid can be linked to Cu. 2+ , Zn 2+ , Ca 2+ , Co 2+ or Fe 3+ These metal chelates can then bind with great strength to proteins and peptides that contain, for example, histidine or cysteine. As a result, the cores bearing the metal chelates can be non-covalently bound to the neoantigenic construct. Preferably, the neoantigenic construct is covalently attached to the core. An example of associating the neoantigenic construct with the core is shown in Example 1.

[0079] The phagocytizable particles of the invention comprise a core and a neoantigenic construct tightly associated with the core. The phagocytizable particles of the invention may comprise one or more neoantigenic constructs associated with the core. For example, the phagocytizable particles of the present invention may comprise 1 to 3 million neoantigenic constructs, preferably 1 to 2 million neoantigenic constructs, more preferably 1 to 1 million neoantigenic constructs, such as 1 to 800,000, 1 to 500,000, 1 to 100,000, 1 to 10,000, 1 to 1000, 1 to 100 or 1 to 10 neoantigenic constructs, or, for example, 10 to 1 million, 100 to 1 million, 1000 to 1 million, 10,000 to 1 million, 100,000 to 1 million, or 500,000 to 1 million neoantigenic constructs. Preferably, the phagocytizable particles of the present invention may comprise 500,000 to 1 million neoantigenic constructs.

[0080] In a preferred embodiment, to maximize delivery of neoantigenic constructs to APCs (which can then be cleaved from the phagocytizable particle and processed by APCs, resulting in the presentation of a wide variety of neoepitope-derived peptides on the surface of APCs), the phagocytizable particles of the invention can comprise two or more neoantigenic constructs associated with the core (i.e., two or more, e.g., 2-3 million). For example, the phagocytizable particles of the invention can comprise 2 to 1 million neoantigenic constructs tightly associated with the core (e.g., 2-800,000, 2-500,000, 2-100,000, 2-1000, 2-100, or 2-10 neoantigenic constructs tightly associated with the core). Preferably, the phagocytizable particles of the invention comprise 10 or more neoantigenic constructs tightly associated with the core, such as 10 to 1 million neoantigenic constructs tightly associated with the core (e.g., 10 to 800,000, 10 to 500,000, 10 to 100,000, 10 to 10,000, 10 to 1000, or 10 to 100 neoantigenic constructs tightly associated with the core). More preferably, the phagocytizable particles of the present invention comprise 100 or more neoantigenic constructs tightly associated with the core, such as 100 to 1 million neoantigenic constructs tightly associated with the core (e.g., 100 to 800,000, 100 to 500,000, 100 to 100,000, 100 to 10,000, or 100 to 1000 neoantigenic constructs tightly associated with the core). In one embodiment, the phagocytizable particles of the present invention comprise 1000 or more genetic constructs tightly associated with the core, such as 1000 to 1 million neoantigenic constructs tightly associated with the core (e.g., 1000 to 800,000, 1000 to 500,000, 1000 to 100,000, or 1000 to 10,000 neoantigenic constructs tightly associated with the core).In certain embodiments, the phagocytizable particles of the invention comprise more than 10,000 neoantigenic constructs tightly associated with the core, such as 10,000 to 1 million neoantigenic constructs tightly associated with the core (e.g., 10,000 to 800,000, 10,000 to 500,000, or 10,000 to 100,000 neoantigenic constructs tightly associated with the core). In certain embodiments, the phagocytizable particles of the invention comprise more than 100,000 neoantigenic constructs tightly associated with the core, such as 100,000 to 1 million neoantigenic constructs tightly associated with the core (e.g., 100,000 to 800,000 or 100,000 to 500,000 neoantigenic constructs tightly associated with the core). In one highly preferred embodiment, the phagocytizable particle of the invention comprises more than 500,000 neoantigenic constructs tightly associated with the core, such as 500,000 to 1 million neoantigenic constructs tightly associated with the core, or 500,000 to 2 million neoantigenic constructs tightly associated with the core, or 500,000 to 3 million neoantigenic constructs tightly associated with the core. In another embodiment, the phagocytizable particle of the invention may comprise more than 1 million neoantigenic constructs tightly associated with the core, such as 1 to 3 million or 1 to 2 million neoantigenic constructs tightly associated with the core.

[0081] In an embodiment of the invention, where a phagocytizable particle of the invention may comprise two or more neoantigenic constructs associated with the core (e.g., two or more, 10 or more, 100 or more, 1000 or more, 10,000 or more, 100,000 or more, or 500,000 or more neoantigenic constructs associated with the core), the neoantigenic constructs associated with the core may be the same or different (i.e., some or all of the neoantigenic constructs associated with the core may be different). They may differ by comprising different neoepitope peptide sequences, or by comprising different combinations of neoepitope peptides. Alternatively or additionally, they may differ by comprising one or more different spacer moieties or additional amino acid sequences (if such moieties and sequences are present). Different neoantigenic constructs may be referred to as "different types" of neoantigenic constructs. Identical neoantigenic constructs may be referred to as "same type" of neoantigenic constructs. Cancer cells often induce multiple amino acid mutations in multiple proteins or peptides expressed by the cancer cells. We have found that phagocytosable particles containing antigenic constructs can deliver a wide variety of neoepitopes to APCs, thereby increasing the diversity of neoepitope-derived peptides presented by APCs. We have found that this significantly improves the activation and proliferation of anti-cancer T cells that can target cancer cells.

[0082] A phagocytizable particle comprising two or more neoantigenic constructs associated with a core of the present invention (e.g., two or more, 10 or more, 100 or more, 1000 or more, 10,000 or more, 100,000 or more, or 500,000 or more neoantigenic constructs associated with a core) can comprise one type of neoantigenic construct tightly associated with the core (i.e., all neoantigenic constructs tightly associated with the core are the same). In one embodiment of the present invention, the phagocytizable particle comprises between 100,000 and 1 million neoantigenic constructs tightly associated with the core, where the 100,000 to 1 million neoantigenic constructs are the same type of neoantigenic construct.

[0083] In alternative embodiments of the invention, a phagocytizable particle comprising two or more neoantigenic constructs associated with the core (e.g., two or more, 10 or more, 100 or more, 1000 or more, 10,000 or more, 100,000 or more, or 500,000 or more neoantigenic constructs associated with the core) may comprise two different neoantigenic construct types tightly associated with the core. In another embodiment of the invention, a phagocytizable particle comprising eleven or more neoantigenic constructs associated with the core (e.g., 100 or more, 1000 or more, 10,000 or more, 100,000 or more, or 500,000 or more neoantigenic constructs associated with the core) may comprise two or more different neoantigenic construct types tightly associated with the core. For example, such phagocytizable particles may comprise 2 to 10 (e.g., 2, 3, 4, 5, 6, 7, 8, 9 or 10) different neoantigenic construct types tightly associated with the core. Preferably, such phagocytizable particles may comprise 2 to 6 different neoantigenic construct types (e.g., 2, 3, 4, 5 or 6). In one embodiment of the invention, the phagocytizable particles comprise 100,000 to 1 million neoantigenic constructs tightly associated with the core, the 100,000 to 1 million neoantigenic constructs comprising two or more different neoantigenic construct types. For example, 2 to 6 different neoantigenic construct types (e.g., 2, 3, 4, 5 or 6).

[0084] In one embodiment of the present invention, the phagocytizable particle further comprises an adjuvant tightly associated with the core. The term "adjuvant" as used herein is understood as any substance that enhances the immune response to an antigen. Specific examples of adjuvants include dsRNA analogs such as polyinosinic:polycytidylic acid, incomplete Freund's adjuvant, cytokines (e.g., IL-2, IL-4, IL-17 and IL-15), CD40, keyhole limpet hemocyanin, Toll-like receptors, CpG oligodeoxynucleotides, saponin, colloidal alum, and analogs of lipid A of lipopolysaccharide. The adjuvant can be tightly associated with the core in the same manner as described herein for tightly associating the neoantigenic construct with the core.

[0085] Sterilization of phagocytizable particles The inventors have found that advantageously, phagocytizable particles comprising a core and a neoantigenic construct tightly associated with the core can be effectively washed and sterilized before administration to a subject. This is particularly advantageous because the washed and sterilized phagocytizable particles contain lower levels of contaminants, such as pathogens (e.g., bacteria, fungi and viruses) and endotoxins (e.g., lipopolysaccharides) and other antigenic contaminants. Such contaminants may induce non-specific immune responses in the subject. Thus, washing and sterilization of the phagocytizable particles of the present invention before administration improves their safety and efficacy.

[0086] In one embodiment of the present invention, the phagocytizable particles include a magnetic core, such as a paramagnetic core or a superparamagnetic core. The phagocytizable particles that include a magnetic core can be collected and / or held in place by a magnet. Washing can also be performed by other means, such as by holding the phagocytizable particles (whether paramagnetic or not) in a column, or by allowing the particles to settle by gravity or centrifugation.

[0087] The particular method of washing is not critical in the context of the present invention. For example, washing can include exposing the phagocytizable particles to high pH, ​​low pH, high temperature, sterilization / denaturing agents, or combinations thereof.

[0088] Washing may include exposing the phagocytizable particles to an alkali, preferably a strong alkali, such as at least 0.1M, 0.5M, 1M, 2M, 3M, 4M, 5M, 6M, 7M, or 8M. Preferably, washing may include exposing the phagocytizable particles to at least 1M sodium hydroxide (NaOH), such as at least 2M NaOH. Preferably, washing includes exposing the phagocytizable particles to a high pH of at least 13.0, more preferably at least 14.0, and most preferably at least 14.3. Other alkalis that may be used include, but are not limited to, lithium hydroxide (LiOH), potassium hydroxide (KOH), rubidium hydroxide (RbOH), cesium hydroxide (CsOH), magnesium hydroxide (Mg(OH)2), calcium hydroxide (Ca(OH)2), strontium hydroxide (Sr(OH)2), and barium hydroxide (Ba(OH)2). Preferably, washing involves exposing the phagocytizable particles to a high pH of at least 13.0, more preferably at least 14.0, and most preferably at least 14.3.

[0089] Washing may also include exposing the phagocytizable particles to an acid, preferably a strong acid, such as an acid of at least 0.1 M, 0.5 M, 1 M, 2 M, 3 M, 4 M, 5 M, 6 M, 7 M, or 8 M. Preferably, washing may include exposing the phagocytizable particles to at least 1 M hydrochloric acid (HCl), such as at least 2 M HCl. Other acids that may be used include, but are not limited to, hydroiodic acid (HI), hydrobromic acid (HBr), perchloric acid (HClO4), nitric acid (HNO3), and sulfuric acid (H2SO4).

[0090] Washing may also include exposing the phagocytizable particles to further sterilizing / denaturing agents such as urea and / or guanidine-HCl.

[0091] Preferably, washing renders the phagocytizable particles aseptic and / or sterile. More preferably, washing renders the phagocytizable particles sterile. Aseptic, as defined herein, is free of disease-causing microorganisms and viruses. Sterile, as defined herein, is free of all biological contaminants.

[0092] Preferably, washing also removes antigenic contaminants, such as pyrogens (e.g., endotoxins) from the phagocytizable particles. Preferably, washing results in phagocytizable particles having less than 100 pg / ml, preferably less than 50 pg / ml, more preferably less than 25 pg / ml, and most preferably less than 10 pg / ml of endotoxin contaminants.

[0093] Thus, in a preferred embodiment of the present invention, the phagocytizable particles are sterile and have less than 100 pg / ml endotoxin contamination.

[0094] A notable advantage of washing is that conditions can be selected such that the phagocytizable particles are sterilized and denatured in a single step. In particular, high pH washing (e.g., pH>14) can conveniently and simultaneously sterilize the phagocytizable particles and remove sufficient amounts of endotoxins and other antigenic contaminants.

[0095] The washing may include one wash or several repeated washes, for example, 2, 3, 4 or 5 washes. Additionally or alternatively, the phagocytizable particles may be exposed to an elevated temperature, for example, at least 90°C, preferably at least 92°C, more preferably at least 95°C, for example at least 100°C or at least 110°C.

[0096] The inventors have advantageously found that when the neoantigenic construct is covalently associated with the core, the phagocytizable particles can withstand rigorous sterilization and washing procedures that reduce the amount of antigenic contaminants such as pyrogens (e.g. endotoxins) that may bind to the neoantigenic construct or core, meaning that the phagocytizable particles described herein are particularly suitable for use in the treatment or prevention of cancer.

[0097] Composition for injection The present invention provides an injectable composition comprising a phagocytizable particle of the present invention.

[0098] The injectable composition of the invention comprising the phagocytizable particles described herein may comprise one or more phagocytizable particles. Preferably, it comprises two or more particles. In such embodiments, the phagocytizable particles may be the same or different. The phagocytizable particles may differ by their cores and / or by comprising different types of neoantigenic constructs tightly associated with the cores. Preferably, in compositions comprising the phagocytizable particles of the invention in which the phagocytizable particles are different, the phagocytizable particles have the same core and differ because the particles comprise different types of neoantigenic constructs tightly associated with the cores.

[0099] Phagocytable particles having different cores (e.g., cores having different sizes and / or comprising different materials / polymers as described herein) are referred to herein as "different sets" of phagocytable particles. Phagocytable particles having the same core (e.g., cores having the same size and comprising the same material / polymer) are referred to herein as the "same set" of phagocytable particles.

[0100] Phagocytotic particles that have the same core (i.e., they are the same set of phagocytotic particles), but that differ because they have different types of neoantigenic constructs tightly associated with the core, are referred to herein as "different groups" of phagocytotic particles. Phagocytotic particles that have the same core and the same types of neoantigenic constructs tightly associated with the core are referred to herein as "same group" of phagocytotic particles.

[0101] In one embodiment, the injectable composition of the present invention comprises a phagocytizable particle set consisting of one phagocytizable particle group (i.e., all of the phagocytizable particles in the composition are the same). Alternatively, the injectable composition of the present invention may comprise a phagocytizable particle set comprising two or more different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 20, 25, 30, 40, or 50 or more phagocytizable particle groups). In one embodiment, the injectable composition of the present invention may comprise a phagocytizable particle set comprising 2 to 50 different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 18, 20, 25, 30, 40, or 50 phagocytizable particle groups). In one embodiment, the injectable composition of the present invention may comprise a single phagocytizable particle set comprising 2 to 30 different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 18, 20, 25, or 30 phagocytizable particle groups). In another embodiment, the injectable composition of the present invention may comprise a single phagocytizable particle set comprising 2 to 20 different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 18, or 20 phagocytizable particle groups). In another embodiment, the injectable composition of the present invention may comprise a single phagocytizable particle set comprising 2 to 15 different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 18, or 20 phagocytizable particle groups). In another embodiment, the injectable composition of the present invention may comprise one phagocytizable particle set comprising 2 to 10 different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 12 or 15 phagocytizable particle groups). In another embodiment, the injectable composition of the present invention may comprise one phagocytizable particle set comprising 2 to 8 different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9 or 10 phagocytizable particle groups). In another embodiment, the injectable composition of the present invention may comprise one phagocytizable particle set comprising 2 to 6 different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9 or 10 phagocytizable particle groups).

[0102] In one embodiment, the injectable composition of the present invention can include two or more different phagocytizable particle sets (i.e., each set has a different core, e.g., each set has a different size core, and / or includes different materials as described herein), and each set can consist of one phagocytizable particle group. For example, the injectable composition of the present invention can include 2, 3, 4 or 5 phagocytizable particle sets, and each set can consist of one phagocytizable particle group. Preferably, the injectable composition of the present invention can include 2 or 3 phagocytizable particle sets, and each set consists of one phagocytizable particle group.

[0103] In another embodiment, the injectable composition of the present invention can include two or more different phagocytizable particle sets (e.g., 2, 3, or 4 phagocytizable particle sets), each set can include two or more different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 20, 25, or 30 phagocytizable particle groups). In one embodiment, the injectable composition of the present invention can include two or more different phagocytizable particle sets (e.g., 2, 3, or 4 phagocytizable particle sets), each set can include from 2 to 30 different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 18, 20, 25, or 30 phagocytizable particle groups). In another embodiment, the injectable composition of the present invention can include two or more different phagocytizable particle sets (e.g., 2, 3, or 4 phagocytizable particle sets), each set can include 2 to 20 different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 18, or 20 phagocytizable particle groups). In another embodiment, the injectable composition of the present invention can include two or more different phagocytizable particle sets (e.g., 2, 3, or 4 phagocytizable particle sets), each set can include 2 to 15 different phagocytizable particle groups (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, or 15 phagocytizable particle groups). In another embodiment, the injectable composition of the present invention can include two or more different phagocytable particle sets (e.g., two, three or four phagocytable particle sets), each set can include two to ten different phagocytable particle groups (e.g., two, three, four, five, six, seven, eight, nine or ten phagocytable particle groups). In another embodiment, the injectable composition of the present invention can include two or more different phagocytable particle sets (e.g., two, three or four phagocytable particle sets), each set can include two to eight different phagocytable particle groups (e.g., two, three, four, five, six, seven or eight phagocytable particle groups). In another embodiment, the injectable composition of the present invention can include two or more different phagocytable particle sets (e.g., two, three or four phagocytable particle sets), each set can include two to six different phagocytable particle groups (e.g., two, three, four, five or six phagocytable particle groups).

[0104] For the avoidance of doubt, in embodiments having more than one group of phagocytizable particles and more than one set of phagocytizable particles, each group of a set of phagocytizable particles is independent of each group of another set of phagocytizable particles. Thus, a group from one set of phagocytizable particles can have the same type of neoantigenic construct as a group from another set of phagocytizable particles. Alternatively, a group from one set of phagocytizable particles can have a different type of neoantigenic construct than a group from another set of phagocytizable particles.

[0105] Cancer cells often induce multiple amino acid mutations in multiple proteins or peptides expressed by cancer cells. Administration of an injectable composition comprising two or more groups of phagocytizable particles allows a wide variety of neoepitopes to be delivered to APCs, thereby increasing the diversity of neoepitope-derived peptides presented by APCs. Increasing the diversity of neoepitope-derived peptides presented by APCs can significantly improve the activation and proliferation of anti-cancer T cells.

[0106] Pharmaceutically acceptable injectable formulations useful according to the present invention include those suitable for parenteral administration, including subcutaneous, intradermal, intramuscular, intravenous (bolus or infusion), intraarticular, and intralymphatic. The most suitable route may depend, for example, on the condition and disease of the recipient. Preferably, the pharmaceutical composition is suitable for intravenous and / or intralymphatic administration. Thus, the present invention provides an injectable composition comprising the phagocytizable particles of the present invention.

[0107] Preferably, the injectable composition of the present invention is an injectable pharmaceutical composition. The injectable composition of the present invention includes compositions suitable for subcutaneous administration, intradermal administration, intramuscular administration, intravenous administration (bolus or infusion), intratumoral administration, intraarticular administration and intralymphatic administration, although the most suitable route may depend, for example, on the type of cancer or tumor present in the subject. Preferably, the injectable composition is suitable for intravenous administration and / or intralymphatic administration.

[0108] The pharma- ceutically acceptable injectable formulations and injectable compositions of the present invention may include aqueous and non-aqueous sterile injection solutions (e.g., physiological saline such as PBS), and may contain aqueous and non-aqueous sterile suspensions that may contain antioxidants, buffers (e.g., sodium phosphate, potassium phosphate, TRIS and TEA), bacteriostats, surfactants (e.g., poloxamer, polysorbate, CHAPS and Titon X-100), and solutes that render the compositions isotonic with the blood of the intended recipient, as well as suspending and thickening agents. The compositions may be presented in unit-dose or multi-dose containers, for example, sealed ampoules and vials, and may be stored in a freeze-dried (lyophilized) condition requiring only the addition of a sterile liquid carrier, for example, physiological saline or water for injection, immediately prior to use. Extemporaneous injection solutions and suspensions for parenteral administration include injectable solutions or suspensions which may contain a suitable non-toxic parenterally-acceptable diluent or solvent, such as, for example, mannitol, 1,3-butanediol, water, Ringer's solution, isotonic sodium chloride solution, or other suitable dispersing or wetting agents and suspending agents, including synthetic mono- or diglycerides, and fatty acids, including oleic acid or cremaphor.

[0109] The pharma- ceutically acceptable formulation of the present invention and the injectable composition of the present invention may further comprise an adjuvant. The term "adjuvant" as used herein is understood as any substance that enhances the immune response to an antigen. Examples of adjuvants for use in the present invention include dsRNA analogs such as polyinosinic:polycytidylic acid, incomplete Freund's adjuvant, cytokines (e.g., interleukins), CD40, keyhole limpet hemocyanin, Toll-like receptors, CpG oligodeoxynucleotides, saponin, colloidal alum, and analogs of lipid A of lipopolysaccharide. Thus, injectable compositions of the invention may include dsRNA analogs such as polyinosinic:polycytidylic acid, incomplete Freund's adjuvant, cytokines (e.g., IL-2, IL-4, IL-17 and IL-15), CD40, keyhole limpet hemocyanin, Toll-like receptors, CpG oligodeoxynucleotides, saponin, colloidal alum, and analogs of lipid A of lipopolysaccharide.

[0110] The preferred unit dosage of the phagocytizable particles of the phagocytizable particles is a therapeutic dose (i.e., a dose sufficient to induce a primary immune response against cancer). The unit dose of the phagocytizable particles may be from 1 μg to 4000 μg, from 10 μg to 3000 μg, or from 10 μg to 2000 μg. For example, the unit dose may be from 10 μg to 1000 μg, 10 μg to 750 μg, 10 μg to 500 μg, 20 to 400 μg, 25 μg to 300 μg, or 30 μg to 200 μg, 50 μg to 1000 μg, 50 μg to 750 μg, 50 μg to 500 μg, 50 μg to 400 μg, 50 μg to 300 μg, 50 μg to 200 μg, 100 μg to 1000 μg, 100 μg to 750 μg, 100 μg to 500 μg, 100 μg to 400 μg, 100 μg to 300 μg or 100 μg to 200 μg, 200 μg to 1000 μg, 200 μg to 750 μg, 200 μg to 500 μg, 200 μg to 400 μg, 200 μg to 300 μg, 400 μg to 1000 μg, 400 μg to 750 μg, 400 μg to 500 μg, 500 μg to 1000 μg, 500 μg to 750 μg, or 750 μg to 1000 μg. For example, the unit dose of the phagocytizable particles can be 1, 10, 50, 100, 200, 250, 300, 400, 500, 600, 700, 750, 800, 900, 1000, 1500, 2000, 3000 or 4000 μg. Preferably, the unit dose is 100 to 750 μg, more preferably 200 to 750 μg, more preferably 300 to 750 μg, more preferably 400 to 750 μg, more preferably 500 to 750 μg, more preferably 600 to 750 μg, even more preferably 650 to 750 μg. For example, the unit dose of the phagocytizable particles can be 100, 200, 300, 400, 500, 600, 700 or 750 μg.

[0111] It will be understood that in addition to the ingredients particularly mentioned above, the pharma- ceutically acceptable formulations of the invention and the injectable compositions of the invention may contain other agents conventional in the art having regard to the type of composition in question.

[0112] The phagocytizable particles of the present invention for use in various embodiments of the present invention can be used as the sole active ingredient, but the phagocytizable particles can also be used in combination with one or more additional active agents.As mentioned above, the present invention also provides phagocytizable particles for use in treating or preventing cancer in a subject, or for use in a method for treating or preventing cancer in a subject, together with additional active agents for simultaneous, sequential or separate administration according to the present invention. Such additional active agents can be drugs or other medicament active materials useful for treating cancer, but are preferably drugs known for treating cancer. Such drugs are known in the art. Examples of additional active agents include alkylating agents, antimetabolites, antitumor antibiotics, histone deacetylase inhibitors, immunomodulators, microtubule interacting drugs, protein kinase inhibitors, steroids, topoisomerase inhibitors, cell cycle inhibitors and angiogenesis inhibitors.

[0113] Thus, the phagocytizable particles of the present invention for use in treating or preventing cancer in a subject, or for use in a method of treating or preventing cancer in a subject of the present invention, may be administered together with one or more compounds known for treating or preventing cancer, such as one or more alkylating agents, antimetabolites, antitumor antibiotics, histone deacetylase inhibitors, immunomodulatory agents, microtubule interacting agents, protein kinase inhibitors, steroids, topoisomerase inhibitors, cell cycle inhibitors, or angiogenesis inhibitors.

[0114] When used in combination, the exact dosage of the additional active agent will vary depending on the administration schedule, the oral potency of the particular agent selected, the age, size, sex and condition of the subject (typically a mammal or human), the nature and severity of the cancer, and other relevant medical and physical factors. Thus, the exact therapeutic or booster dose cannot be specified in advance and can be readily determined by the caregiver or clinician. Appropriate amounts can be determined using animal models. and can be determined by routine experimentation from human clinical studies. In humans, therapeutic or booster doses are known or otherwise determined by one of ordinary skill in the art.

[0115] The individual components of such combinations can be administered separately at different times during the course of therapy or concurrently in divided or single combination forms, and the invention is therefore to be understood as embracing all such regimes of simultaneous or alternating treatment.

[0116] The above-mentioned additional active agents, when used in combination with the compounds useful in the present invention, may be used, for example, in amounts as indicated in the Physician's Package Insert (PDR) or as otherwise determined by one of skill in the art.

[0117] treatment The present invention provides a phagocytizable particle for use in treating or preventing cancer in a subject, the phagocytizable particle comprising a core and a neoantigenic construct tightly associated with the core, the neoantigenic construct comprising a neoepitope peptide having an amino acid sequence corresponding to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by cancer cells in the subject, the portion of the protein or peptide having at least one somatically mutated amino acid. The present invention also provides a method of treating or preventing cancer in a subject, comprising administering to the subject a phagocytizable particle of the present invention. The present invention also provides a use of the phagocytizable particle of the present invention for the manufacture of a medicament for treating or preventing cancer.

[0118] The inventors have found that the phagocytizable particles used in the present invention are surprisingly effective in eliciting a robust anti-cancer immune response against cancer cells in a subject. At a general level, immune responses can be classified as either innate or adaptive immune responses. Innate immune responses are immune responses that are essentially unaffected by prior contact with an antigen. Such responses are often characterized by the activation and proliferation of naive T and B cells. In contrast, adaptive immune responses are immune responses that require prior contact with an antigen. Adaptive immune responses generally follow shortly after an innate immune response and ultimately result in immune memory against the antigen. The immune responses (innate and adaptive) that are elicited when the immune system first contacts an antigen are often referred to as "primary immune responses". Subsequent activation of memory T and B cells by the same antigen results in a rapid and specific immune response against the antigen, which is often referred to as "secondary immune response".

[0119] The investors have surprisingly found that the phagocytizable particles of the present invention induce a robust anti-cancer immune response by activating both the innate and adaptive immune responses in a subject.

[0120] The immune response induced by the phagocytosis-capable particles used in the present invention may include phagocytosis of the phagocytosis-capable particles by antigen-presenting cells (APCs). APCs are typically dendritic cells (DCs), B cells or macrophages, or cells that phagocytose or ingest extracellular organisms or proteins (i.e., antigens), and present antigen-derived peptides on MHC class II and / or MHC class I to CD4+ T cells and / or CD8+ T cells after processing. In blood, the most abundant APCs are monocytes, e.g., dendritic cells, macrophages and B cells.

[0121] The immune response induced by the phagocytizable particles used in the present invention may also induce the activation and proliferation of naive or memory T cells. For example, the phagocytizable particles of the present invention may induce the activation and proliferation of CD4+ T cells (or T helper cells or CD4+ helper T cells) and / or CD4+ T cells. CD4+ T cells are cells that direct immune responses through cytokine secretion. They can suppress or enhance other immune cells, such as stimulating antibody class switching in B cells, stimulating activation and proliferation of cytotoxic T cells, or enhancing phagocytes. They are activated by antigen presentation via MHC class II on APCs and express T cell receptors (TCRs) specific for stretches of about 15 amino acids (so-called T cell epitopes) within a particular antigen. CD8+ T cells (or cytotoxic T cells) are cells that kill tumor cells, infected cells or cells that have been otherwise damaged. Unlike CD4+ T cells, they do not require APCs for activation. Their T cell receptors recognize antigen-derived peptides (about 7-10, e.g., 8 amino acids long) presented by MHC class I, a protein expressed on all nucleated cells.

[0122] The treatment of the invention may be used to treat or prevent any form of cancer, such as solid cancer, metastatic solid cancer or hematological malignancies.

[0123] A "solid tumor" herein is, for example, an abnormal mass of tissue derived from an organ. Solid tumors can be malignant. Various types of solid tumors are named for the type of cells that form them. Types of solid tumors include sarcomas, carcinomas, and lymphomas. Examples of solid tumors include adrenal cancer, anal cancer, anaplastic large cell lymphoma, angioimmunoblastic T-cell lymphoma, B-cell lymphoma, bile duct cancer, bladder cancer, brain / CNS tumors, breast cancer, cervical cancer, colon cancer, endometrial cancer, esophageal cancer, ewing family of tumors, eye cancer, gallbladder cancer, gastrointestinal carcinoid tumors, gastrointestinal stromal tumors (gist), gestational trophoblastic disease, hepatosplenic T-cell lymphoma, Hodgkin's lymphoma, intravascular large B-cell lymphoma, kidney cancer, laryngeal and hypopharyngeal cancer, liver cancer, lung cancer (non-small cell and small cell), lung carcinoid tumors These include lymphoid granulomatosis, malignant mesothelioma, nasal cavity and paranasal sinus cancer, nasopharyngeal cancer, neuroblastoma, marginal zone B-cell lymphoma, non-Hodgkin's lymphoma, oral cavity and oropharyngeal cancer, osteosarcoma, ovarian cancer, pancreatic cancer, penile cancer, pituitary tumor, primary humoral lymphoma, prostate cancer, retinoblastoma, rhabdomyosarcoma, salivary gland cancer, sarcoma, skin cancer (basal and squamous cell, melanoma and Merkel cell), small intestine cancer, stomach cancer, testicular cancer, thymic cancer, thyroid cancer, uterine sarcoma, vaginal cancer, vulvar cancer, Waldenstrom's macroglobulinemia, and Wilms' tumor.The treatment of the present invention is particularly effective in the treatment of solid cancer.Therefore, the subject of the present invention can be solid cancer. The treatment of the present invention is particularly effective in the treatment of solid cancers selected from the group consisting of anal cancer, bladder cancer, breast cancer, cervical cancer, colon cancer, liver cancer, lung cancer (non-small cell and small cell), lung carcinoid tumor, ovarian cancer, pancreatic cancer, penile cancer, prostate cancer, gastric cancer, testicular cancer, uterine sarcoma, vaginal cancer, vulvar cancer, and even more specifically in the treatment of breast cancer, colon cancer, liver cancer, lung cancer (non-small cell and small cell), lung carcinoid tumor, pancreatic cancer, prostate cancer, ovarian cancer, and bladder cancer.

[0124] The treatment of the present invention is also particularly effective in treating metastatic solid cancers, which are cancers that have spread from their primary site to one or more different areas of the body.

[0125] Alternatively, the cancer may be any form of hematological malignancy. Hematological malignancies are a form of cancer that begins in the cells of blood-forming tissues, such as the bone marrow or lymphatic system. In many hematological malignancies, the normal blood cell development process is disrupted by the uncontrolled growth of abnormal types of blood cells. Examples of hematological cancers include leukemia, lymphoma, myeloma, and myelodysplastic syndromes (lymphomas can be classified as both solid cancers and hematological malignancies). Examples of hematological malignancies include acute basophilic leukemia, acute eosinophilic leukemia, acute erythrocytic leukemia, acute lymphoblastic leukemia, acute megakaryoblastic leukemia, acute monocytic leukemia, mature acute myeloblastic leukemia, acute myeloid leukemia, acute myeloid dendritic cell leukemia, acute promyelocytic leukemia, adult T-cell leukemia / lymphoma, aggressive NK cell leukemia, anaplastic large cell lymphoma, and plasmacytoma. , angioimmunoblastic T-cell lymphoma, B-cell chronic lymphocytic leukemia, B-cell leukemia, B-cell lymphoma, B-cell prolymphocytic leukemia, chronic idiopathic myelofibrosis, chronic lymphocytic leukemia, chronic myelogenous leukemia, chronic myelomonocytic leukemia, chronic neutrophilic leukemia, extramedullary leukemia, hairy cell leukemia, hepatosplenic T-cell lymphoma, Hodgkin's lymphoma, intravascular large B-cell lymphoma, Kahler's disease, lymphomatoid granulomatosis, mast cell leukemia, multiple myeloma, fascial, nodal marginal zone B-cell lymphoma, non-Hodgkin's lymphoma, plasma cell leukemia, primary effusion lymphoma, and Waldenstrom's macroglobulinemia.

[0126] Administration and Dosing Regimen A therapeutic dose of the phagocytizable particles or injectable compositions of the present invention is a dose sufficient to induce an immune response against cancer in a subject, for example, a primary immune response and / or a secondary immune response.

[0127] In some embodiments of the invention, the use of the phagocytizable particles described herein for the treatment or prevention of cancer comprises administering a therapeutic dose of the phagocytizable particles to a subject. In some embodiments of the invention, the method of treating or preventing cancer of the invention comprises administering a therapeutic dose of the phagocytizable particles to a subject. In some embodiments of the invention, the use of the phagocytizable particles of the invention or the treatment method of the invention comprises administering a therapeutic dose of the injectable composition of the invention to a subject.

[0128] The therapeutic dose of the phagocytizable particles or injectable compositions of the present invention required to treat or prevent cancer in a subject will vary depending on the route of injection and the characteristics of the subject being treated, such as species, age, weight, sex, medical condition, the particular cancer and its severity, and other relevant medical and physical factors. A physician of ordinary skill can readily determine and administer the effective amount of phagocytizable particles required to treat or prevent cancer.

[0129] The therapeutic dose of the phagocytizable particles can be 1 μg to 4000 μg, 10 μg to 3000 μg, or 10 μg to 2000 μg. For example, the dose can be 10 μg to 1000 μg, 10 μg to 750 μg, 10 μg to 500 μg, 20 to 400 μg, 25 μg to 300 μg, 30 μg to 200 μg, 50 μg to 1000 μg, 50 μg to 750 μg, 50 μg to 500 μg, 50 μg to 400 μg, 50 μg to 300 μg, 50 μg to 200 μg, 100 μg to 1000 μg, 100 μg to 750 μg, 100 μg to 500 μg, 1 00 μg to 400 μg, 100 μg to 300 μg or 100 μg to 200 μg, 200 μg to 1000 μg, 200 μg to 750 μg, 200 μg to 500 μg, 200 μg to 400 μg, 200 μg to 300 μg, 400 μg to 1000 μg, 400 μg to 750 μg, 400 μg to 500 μg, 500 μg to 1000 μg, 500 μg to 750 μg, or 750 μg to 1000 μg. For example, the dose of the phagocytizable particles can be 1, 10, 50, 100, 200, 250, 300, 400, 500, 600, 700, 750, 800, 900, 1000, 1500, 2000, 3000, or 4000 μg. Preferably, the dose is 100 to 750 μg, more preferably 200 to 750 μg, more preferably 300 to 750 μg, more preferably 400 to 750 μg, more preferably 500 to 750 μg, more preferably 600 to 750 μg, and even more preferably 650 to 750 μg. For example, the dose of the phagocytizable particles can be 100, 200, 300, 400, 500, 600, 700, or 750 μg.

[0130] Alternatively, the therapeutic dose of the phagocytizable particles can be determined based on the number of phagocytizable particles. For example, the dose can be about 10 4 From 10 10 , 10 5 From 10 9 , 10 5 From 10 8 , 10 5 From 10 7 , or 10 5 From 10 6phagocytosable particles (e.g., 104, 5 5 , 10 5 , 5 6 , 10 6 , 5 7 , 10 7 , 5 8 , 10 8 , 5 9 , 10 9 , 5 10 , or 10 10 Preferably, the dose is approximately 10 5 From 1 0 8 ,10 5 From 10 7 , or 10 5 From 10 6 phagocytosis particles, e.g., approximately 10 5 From 10 9 , 5x10 5 From 10 8 , 5x10 5 From 7.5x10 7 , 5x10 5 From 5x10 7 , 5x10 5 From 2.5x10 7 , or 5x10 5 From 10 7 More preferably, the dose is about 10 7 From 10 9 Pieces, for example, about 5 x 10 7 From 10 9 , 7.5×10 7 From 10 9 , 7.5×10 7 From 7.5 × 10 8 , 7.5×10 7 From 5×10 8 , or 7.5 × 10 7 From 2.5 × 10 8 More preferably, the dose is approximately 7.5×10 7 From 5×10 8 phagocytosis, for example, approximately 75 million, 100 million, 150 million, 200 million, or 250 million phagocytosis particles.

[0131] Alternatively, the therapeutic dose of the phagocytizable particle can be determined based on the amount of neoantigenic construct associated with the core. For example, the dose can be 1 μg to 4000 μg, 10 μg to 3000 μg, or 10 μg to 2000 μg of neoantigenic construct. For example, 1 μg to 4000 μg, 10 μg to 3000 μg, or 10 μg to 2000 μg. For example, the dose of the neoantigenic construct may be from 10 μg to 1000 μg, 10 μg to 750 μg, 10 μg to 500 μg, 10 μg to 400 μg, 10 μg to 300 μg, 10 μg to 200 μg, 10 μg to 100 μg or 10 to 50 μg, 10 to 25, 20 μg to 400 μg, 25 μg to 300 μg, 30 μg to 200 μg, 50 μg to 1000 μg, 50 μg to 750 μg, 50 μg to 500 μg, 50 μg to 400 μg, 50 μg to 300 μg or 50 μg to 200 μg, 00μg to 1000μg, 100μg to 750μg, 100μg to 500μg, 100μg to 400μg, 100μg to 300μg, or 100μg to 200μg, 200μg to 1000μg, 200μg to 750μg, 200μg to 500μg, 200μg to 400μg, 200μg to 300μg, 400μg to 1000μg, 400μg to 750μg, 400μg to 500μg, 500μg to 1000μg, 500μg to 750μg, or 750μg to 1000μg. For example, the dose of neoantigenic construct may be 1, 10, 15, 20, 25, 30, 40, 50, 75, 100, 200, 250, 300, 400, 500, 600, 700, 750, 800, 900, 1000, 1500, 2000, 3000, or 4000 μg. Preferably, the dose may be 10 μg to 1000 μg, 10 μg to 750 μg, 10 μg to 500 μg, 10 μg to 400 μg, 10 μg to 300 μg, 10 μg to 200 μg, 10 μg to 100 μg, or 10 to 50 μg of neoantigenic construct. Preferably the dose is between 10 μg and 100 μg of the neoantigenic construct, for example between 10 μg and 75 μg, 10 μg and 50 μg, 10 μg and 25 μg, 25 μg and 50 μg, or 50 μg and 75 μg of the neoantigenic construct.For example, the dose of the phagocytizable particles can be 1, 10, 15, 20, 25, 30, 40, 50, 75, 100 μg of neoantigenic construct. Preferably, the dose is between 10 and 50 μg of neoantigenic construct.

[0132] The therapeutic dose may be administered as a single unit dose comprising a therapeutic dose of the phagocytizable particles, or as multiple unit doses where the unit dose comprises a fraction of the therapeutic dose. Preferably, a therapeutic dose of the phagocytizable particles of the present invention is administered to a subject as a single dose.

[0133] A therapeutic dose of phagocytizable particles, or an injectable composition comprising a therapeutic dose of phagocytizable particles of the invention, can be administered to a subject once.

[0134] In a preferred embodiment, a subject is administered a therapeutic dose of phagocytizable particles or an injectable composition comprising a therapeutic dose of phagocytizable particles, and at least one additional (or "subsequent") therapeutic dose of phagocytizable particles of the invention or an injectable composition comprising a therapeutic dose of phagocytizable particles of the invention. The additional (or "subsequent") therapeutic dose of phagocytizable particles may be administered daily, every second or third day, weekly, every two, three or four weeks, monthly, bimonthly, or monthly. The number and frequency of additional therapeutic doses of the phagocytizable particles of the invention or an injectable composition comprising a therapeutic dose of the phagocytizable particles of the invention will depend on the subject and the type and severity of the cancer being treated.

[0135] In one embodiment, the use of phagocytizable particles for the treatment or prevention of cancer comprises administering one or more subsequent therapeutic doses of the phagocytizable particles of the present invention or an injectable composition comprising a therapeutic dose of the phagocytizable particles of the present invention to a subject, the subject having previously been administered a therapeutic dose of the phagocytizable particles of the present invention or an injectable composition comprising a therapeutic dose of the phagocytizable particles of the present invention, each of the one or more subsequent therapeutic doses being a dose sufficient to induce an immune response (i.e., a primary immune response and / or a secondary immune response) against cancer cells in the subject.

[0136] In embodiments of the invention that involve administering to a subject one or more subsequent therapeutic doses of a phagocytizable particle of the invention or an injectable composition comprising a therapeutic dose of a phagocytizable particle of the invention, preferably 2, 3, 4, 5, 6, 7, 8, 9, 10 or n subsequent doses of the phagocytizable particle of the invention. Subsequent therapeutic doses of the phagocytizable particles or injectable composition are administered to the subject, where "n" is any number of doses greater than 10 (e.g., 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 doses). Preferably, the number and frequency of subsequent therapeutic doses administered to the subject is sufficient to treat or prevent cancer in the subject.

[0137] A therapeutic dose of a phagocytizable particle or injectable composition of the present invention may independently have any of the properties and / or characteristics of a therapeutic dose of a phagocytizable particle or injectable composition described herein. In a preferred embodiment of the present invention, the phagocytizable particle administered to the subject as a subsequent therapeutic dose comprises the same type of neoantigenic construct as the phagocytizable particle previously administered to the subject as a therapeutic dose. The core of the phagocytizable particle administered to the subject as a subsequent therapeutic dose may be the same or different from the core of the phagocytizable particle previously administered to the subject as a therapeutic dose (e.g., the core may have a different size and / or may comprise a different material / polymer as described herein). Preferably, the phagocytizable particle administered to the subject as a subsequent therapeutic dose may comprise the same type of neoantigenic construct and the same core as the phagocytizable particle previously administered to the subject as a therapeutic dose (i.e., the phagocytizable particles are the same set and are the same for each group previously administered to the subject as a therapeutic dose).

[0138] In the embodiment of the present invention that includes administering one or more subsequent therapeutic doses of phagocytizable particles or an injectable composition that includes a therapeutic dose of phagocytizable particles to a subject, preferably, one or more subsequent therapeutic doses are administered to the subject at intervals of several days, weeks, or months. For example, one or more subsequent therapeutic doses are administered to the subject every day, every 2nd day, every 3rd day, every 4th day, every 5th day, or every 6th day. Alternatively or additionally, one or more subsequent therapeutic doses are administered to the subject, for example, once a week, once every 2 weeks, once every 3 weeks, or once every 4 weeks. Alternatively or additionally, one or more subsequent therapeutic doses are administered to the subject, for example, once a month, once every 2 months, once every 3 months, or once every 6 months. Alternatively or additionally, one or more subsequent therapeutic doses are administered to the subject, for example, once a year.

[0139] In one embodiment of the present invention, one or more subsequent therapeutic doses are administered to the subject once per year, twice per year, or three times per year. For example, one or more subsequent therapeutic doses can be administered to the subject once per year, twice per year, or three times per year for a period of 1 to 10 years, 1 to 20 years, 1 to 30 years, 1 to 40 years, or 1 to 60 years (e.g., a period of 1 to 10 years, 10 to 20 years, 20 to 30 years, 30 to 40 years, or 50 to 60 years).

[0140] The inventors have demonstrated that administering one or more subsequent therapeutic doses to a subject over an extended period (i.e., over a period of one year or more) can enhance the proliferation and proliferation of anti-cancer memory B cells and melanoma cells present in the subject. It has been found that it is possible to increase the number of anti-cancer memory B cells and memory T cells in a subject. It is expected that by increasing the number of anti-cancer memory B cells and memory T cells in a subject, anti-cancer immune memory in the subject will be maintained, thereby preventing the growth (e.g., formation of tumors) or recurrence of cancer.

[0141] In certain embodiments of the invention, a booster dose is administered to a subject whose cancer has been successfully treated (with one or more therapeutic doses described herein) to prevent recurrence of the cancer.

[0142] A booster dose of the phagocytizable particles or injectable compositions of the present invention may independently have any of the properties and / or characteristics of the therapeutic dose of the phagocytizable particles or injectable compositions described herein. In a preferred embodiment of the present invention, the phagocytizable particles administered to the subject as a booster dose contain the same type of neoantigenic construct as the phagocytizable particles administered to the subject as a therapeutic dose. The core of the phagocytizable particles may be the same or different from the core of the phagocytizable particles administered to the subject as a therapeutic dose (e.g., the core may have a different size and / or may contain a different material / polymer as described herein). Preferably, the booster dose contains phagocytizable particles having the same type of neoantigenic construct and the same core as the phagocytizable particles administered to the subject as a therapeutic dose (i.e., the phagocytizable particles are the same set and are the same as each group previously administered to the subject as a therapeutic dose).

[0143] In one embodiment of the present invention, one or more booster doses are administered to the subject, for example, once a week, once every two weeks, once every three weeks, or once every four weeks.Alternatively or additionally, one or more booster doses are administered to the subject, for example, once a month, once every two months, once every three months, or once every six months.Alternatively or additionally, one or more booster doses are administered to the subject, for example, once a year.

[0144] In another embodiment of the invention, one or more booster doses are administered to a subject once per year, twice per year, or three times per year. For example, one or more booster doses can be administered to a subject once per year, twice per year, or three times per year for a period of 1 to 10 years, 10 to 20 years, 20 to 30 years, 30 to 40 years, or 50 to 60 years.

[0145] Increasing the number of anti-cancer memory B cells and memory T cells in a subject by administering one or more booster doses is expected to maintain anti-cancer immune memory in the subject, thereby preventing the cancer from growing or recurring.

[0146] Ex vivo activation and expansion of anti-cancer T cells The invention also provides a treatment of the invention comprising the additional steps of: a) harvesting APCs and anti-cancer T cells from a subject; b) expanding the anti-cancer T cells harvested from the subject; and c) administering a therapeutic dose of the expanded anti-cancer T cells to the subject.

[0147] The present invention also provides a method of treating or preventing cancer in a subject, the method comprising the additional steps of: a) harvesting APCs and anti-cancer T cells from the subject; b) expanding the anti-cancer T cells harvested from the subject; and c) administering a therapeutic dose of the expanded anti-cancer T cells to the subject. Step a) collecting APCs and anti-cancer T cells from the subject after administration of phagocytosis-capable particles to the subject.

[0148] In one embodiment of the present invention, in step a), the APCs and anti-cancer T cells are harvested from the subject after administration of a dose (preferably a therapeutic dose) of the phagocytizable particles or injectable composition of the present invention to the subject. Alternatively or additionally, the APCs and anti-cancer T cells are harvested simultaneously and / or The APCs and anti-cancer T cells may be harvested from a subject before a dose (preferably a therapeutic dose) of the phagocytizable particles or injectable compositions of the present invention is administered to the subject. Preferably, the APCs and anti-cancer T cells are harvested from a subject after a dose (preferably a therapeutic dose) of the phagocytizable particles or injectable compositions of the present invention is administered to the subject.

[0149] APC must be compatible with anti-cancer T cells so that it can present antigen to anti-cancer T cells in an antigen-specific context (MHC-restricted) to which anti-cancer T cells can respond. APC and anti-cancer T cells are preferably obtained from the same species and donor-matched with respect to MHC receptors. However, the use of genetically engineered APC from different species is also envisaged. More preferably, APC and anti-cancer T cells are obtained from the same subject. If APC and anti-cancer T cells are derived from the same subject, the possibility of mismatch between APC and anti-cancer T cells is avoided.

[0150] The APCs obtained from the subject may include phagocytes, monocytes and / or dendritic cells. The anti-cancer T cells may include CD4+ and / or CD8+ T cells.

[0151] The APCs and anti-cancer T cells may be obtained from a blood sample derived from a subject. Preferably, the blood sample is a peripheral blood mononuclear cell (PBMC) sample. PBMCs are a human blood fraction prepared by density gradient centrifugation of whole blood. PBMCs consist primarily of lymphocytes (70-90%) and monocytes (10-30%), with red blood cells, granulocytes and plasma removed. Monocytes may optionally constitute 10 to 20%, e.g. 10 to 15%, of the cell population in a PBMC sample.

[0152] The APC and anti-cancer T cells can also be derived from a sample derived from a tumor of a subject, such as a lymphatic vessel within the tumor or a tumor-draining lymph node (i.e., a sentinel node). Preferably, the APC and anti-cancer T cells are taken from the same sample from a subject and / or from the same tumor of a subject. Alternatively or additionally, the APC and anti-cancer T cells can be taken from different samples from a subject. For example, the APC can be taken from a blood sample and the anti-cancer T cells can be taken from a tumor.

[0153] Preferably, APC and anti-cancer T cells are collected from a PBMC sample. For example, APC and anti-cancer T cells are collected from the same PBMC sample or different PBMC samples. Obtaining PBMC from a peripheral blood sample is a common protocol and provides a convenient source for both APC and T cells at the same time and from the same subject. The PBMC sample may be used freshly collected or may be frozen for storage before use. Step b) Proliferating anti-cancer T cells collected from the subject. The APCs and anti-cancer T cells collected from the subject in step a) can be used to prepare anti-cancer T cells suitable for use in treating or preventing cancer in the subject. Anti-cancer T cells suitable for administration to a subject are prepared by in vitro activation and expansion of the anti-cancer T cells collected from the subject. The in vitro activation and expansion method includes: i) providing a phagocytizable particle comprising a core and a neoantigenic construct tightly associated with the core, the neoantigenic construct comprising a neoepitope peptide having an amino acid sequence corresponding to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by cancer cells in a subject, the portion of the protein or peptide having at least one somatically mutated amino acid; ii) providing APCs; iii) contacting the phagocytosis-capable particle with an APC in vitro under conditions that allow phagocytosis of the phagocytosis-capable particle by the APC; iv) providing anti-cancer T cells collected from a subject; v) contacting in vitro the anti-cancer T cells with the APCs from step iii) under conditions that allow specific activation and proliferation of the anti-cancer T cells in response to the neoepitopes presented by the APCs.

[0154] In vitro expansion methods involve expanding APCs from 100 to 1 × 10 9 phagocytosis particles, e.g., 100 to 1 × 10 8 Pieces, for example 100 to 1×10 7 phagocytosis, in a given propagation run, can include contacting the cells with, for example, 1000 to 1×10 phagocytosis 7 phagocytosis particles are used. For example, the ratio of phagocytosis particles to APC is within the range of 1000:1 to 1:10. The ratio can be optimized according to the size of phagocytosis particles. For example, for phagocytosis particles with a maximum diameter of about 1 μm, the ratio can be within the range of 50:1 to 2:1, for example, 25:1 to 5:1, 15:1 to 7:1, and 10:1.

[0155] For the avoidance of doubt, a phagocytizable particle suitable for contact with an APC may independently have any of the properties and / or characteristics of a phagocytizable particle administered to a subject as a therapeutic dose or a booster dose.

[0156] In one embodiment of the present invention, APCs are contacted with phagocytizable particles that contain the same type of neoantigenic construct as the phagocytizable particles administered to the subject in a therapeutic dose. The core of the phagocytizable particles may be the same or different (e.g., the core may have a different size and / or may comprise a different material / polymer as described herein) as the core of the phagocytizable particles administered to the subject in a therapeutic dose. In a preferred embodiment of the present invention, APCs are contacted with phagocytizable particles that contain the same type of neoantigenic construct and the same core as the phagocytizable particles administered to the subject in a therapeutic dose.

[0157] In one embodiment of the present invention, the method of expanding anti-cancer T cells comprises adding a low dose of IL-2 to the anti-cancer T cell sample, for example, more than 1.25 U / ml (e.g., more than 1.25 U / ml, more than 2.5 U / ml, more than 5 U / ml, or more than 50 U / ml) of IL-2, preferably more than 2.5 U / ml, more than 5 U / ml, or more than 50 U / ml of IL-2. Expansion of antigen-specific T cells occurs in the presence of antigen-presenting cells when IL-2 is simultaneously present. IL-2 promotes differentiation of anti-cancer T cells into effector anti-cancer T cells and memory anti-cancer T cells. After expansion of anti-cancer T cells, APCs can be removed from the expanded T cell population, for example by magnetic separation.

[0158] In another embodiment of the invention, the method of expanding anti-cancer T cells comprises adding IL-2 and / or IL-7 and / or IL-15 to an anti-cancer T cell sample, e.g. adding a low dose of IL-2 to an anti-cancer T cell sample, e.g. adding more than 1.25 U / ml (e.g. more than 1.25 U / ml, more than 2.5 U / ml, more than 5 U / ml, or more than 50 U / ml), preferably more than 2.5 U / ml, more than 5 U / ml, or more than 50 U / ml of IL-2, and optionally adding IL-7 and / or IL-15. For example, a low dose of IL-7 for an anti-cancer T cell sample is, for example, greater than 1.25 U / ml (e.g., greater than 1.25 U / ml, greater than 2.5 U / ml, greater than 5 U / ml, or greater than 50 U / ml), preferably greater than 2.5 U / ml, 5 U / ml, or greater than 50 U / ml of IL-7, and / or, for example, a low dose of IL-15 for an anti-cancer T cell sample is, for example, greater than 1.25 U / ml (e.g., greater than 1.25 U / ml, greater than 2.5 U / ml, greater than 5 U / ml, or greater than 50 U / ml of IL-15.

[0159] In a preferred embodiment of the invention, the method of activating and expanding anti-cancer T cells in vitro comprises removing APCs that have taken up phagocytosis particles of the invention from the anti-cancer T cells. In an embodiment of the invention in which the phagocytosis particles of the invention comprise a magnetic core, the APCs are removed from the anti-cancer T cells by using magnetic separation.

[0160] After contacting the anti-cancer T cells with the APC loaded with the phagocytizable particles of the present invention, the degree of anti-cancer T cell activation can be determined, for example, by comparing the degree of anti-cancer T cell activation with a relevant reference. The degree of anti-cancer T cell activation can be determined using T cell activation assays known in the art, such as ELISpot, FluoroSpot, intracellular staining of cytokines using flow cytometry, FASCIA (flow cytometry assay for specific cell-mediated immune response in activated whole blood), proliferation assays (e.g., thymidine incorporation, CFSE or BrdU staining), specific TCR-detection using MHC-I or II tetramers, and ELISA- or Luminex analysis of ELIS-po assay of secreted cytokines. The method can include a step of comparing the degree of anti-cancer T cell activation with a relevant reference. Suitable references include, for example, T cell samples that do not contain anti-cancer T cells or anti-cancer T cell samples that have not been contacted with the APC loaded with the phagocytizable particles. c) administering to the subject a therapeutic dose of the expanded anti-cancer T cells.

[0161] The expanded anti-cancer T cells can be administered to a subject in a therapeutic dose. Thus, the present invention also provides anti-cancer T cells for use in treating or preventing cancer in a subject. The expanded anti-cancer T cells can be administered to a subject intravenously, intraarterially, intrathecally, or intraperitoneally.

[0162] The exact dosage of the expanded anti-cancer T cells varies according to the administration schedule, the age, physique, sex and condition of the subject (typically a mammal or human), the nature and severity of the condition, and other relevant medical and physical factors.Accurate therapeutically effective amount can therefore be easily determined by clinicians.Appropriate amounts can be determined by routine experiments from animal models and human clinical studies.For humans, effective doses are known or can be otherwise determined by those skilled in the art. EXAMPLES

[0163] Example 1: General protocol for coupling neoantigenic constructs or model peptides / proteins to magnetic cores

[0164] Coupling of neoantigenic constructs or model peptides / proteins to the core Dynabeads® MyOne™ Carboxylic Acid (ThermoFischer Scientific) (1 μm diameter spheres) were used as cores. Dynabeads® MyOne™ Carboxylic Acid particles are paramagnetic polystyrene particles containing iron oxide and functionalized on the particle surface with free carboxylic acid groups. The coupling procedure was performed according to the manufacturer's protocol (two-step procedure using NHS (N-hydroxysuccinimide) and EDC (ethylcarbodiimide)).

[0165] Step 1): Wash twice with MES-Buffer (25 mM MES (2-(N-morpholino)ethanesulfonic acid), pH 6). Then, 50 mg / ml NHS (N-hydroxysuccinimide) and 50 mg / ml EDC (N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide) in MES buffer were added to the polystyrene particles to activate the carboxylic acid groups, and the particles were incubated at room temperature (RT) for 30 minutes. The polystyrene particles were collected using a magnet, the supernatant was removed, and the particles were washed twice with MES buffer.

[0166] Step 2): Neoantigenic constructs or model peptide / protein samples were diluted in MES buffer to a concentration of 1 mg / ml, 100 μg total, added to polystyrene particles and incubated at room temperature for 1 h. The polystyrene particles were collected with a magnet and the supernatant was removed and saved for peptide concentration measurement. Unreacted activated carboxylic acid groups were cleaved with 50 mM Tris pH 7.4. The polystyrene particles were then washed with PBS pH 7.4 and then quenched with -80 ℃ Saved it.

[0167] The amount of peptidic material coupled to the polystyrene particles was measured using a BCA (bicinchoninic acid) protein assay kit (Pierce BCA Protein Assay Kit, ThermoFisher Scientific) according to the manufacturer's protocol, and the peptidic material concentrations of the neoantigenic construct samples before coupling and of the supernatants after coupling were measured.

[0168] Several polypeptides were tested, with an estimated average of 48.7 μg (mean: 48.7, SD: 20.5, N=10) of neoantigenic constructs coupled per mg of polystyrene particles. According to the manufacturer's instructions, 50 μg of polypeptides can be coupled per mg of particles, indicating that a high efficiency of coupling was achieved.

[0169] Example 2: Washing Polystyrene particles were coupled to recombinant neoantigenic constructs produced in E. coli according to the method described in Example 1. After coupling, the polystyrene particles were washed with one of three different wash buffers (2M NaOH pH 14.3, 8M urea, or 6M guanidine (guanidine-HCl), all in sterile water at room temperature) or the polystyrene particles were soaked in water for 95 min. ℃ The polystyrene particles were suspended in the buffer, shaken for 4 minutes, collected with a magnet, and the supernatant was removed. This was repeated three times. The heat-treated polystyrene particles were placed in PBS pH 7.4 and incubated at 95 °C for 1 h. ℃ The particles were placed in a heating block at 400 C for 5 min, then collected with a magnet and the supernatant removed. This was repeated three times. The particles were then washed three times with sterile PBS to remove any remaining wash buffer.

[0170] Four different washing conditions were tested: (a) high pH (2M NaOH pH 14.3), (b) high temperature (95°C) and sterilizing / denaturing agents ((c) 8M urea, and (d) 6M guanidine hydrochloride). In all cases, the neoantigenic constructs associated with the polystyrene particles remained associated with the polystyrene particles.

[0171] Example 3a: Identification of suitable particle size for phagocytosis A cell proliferation assay measuring thymidine incorporation was used to test the effect of phagocytic particle size on antigen-specific T cell activation. Splenocytes from ovalbumin (OVA)-immunized mice were stimulated with OVA-conjugated polystyrene particles of different sizes to measure antigen-specific proliferation.

[0172] Dynabeads® MyOne™ carboxylic acid particles with diameters of 5.6 μm, 1 μm, and 0.2 μm were coupled with OVA (OVA particles) or bovine serum albumin (BSA particles) according to the protocol of Example 1.

[0173] To test the efficacy of OVA particles in stimulating antigen-specific T cell activation, a proliferation assay ( 3 H-thymidine incorporation) was used. Particle to cell concentration was 1:1 for 5.6 μm particles, 10:1 for 1 μm particles, and 500:1 for 0.2 μm particles. Total protein concentrations during incubation with cells were calculated to be 125 ng / ml, 160 ng / ml, and 160 ng / ml for 5.6 μm, 1 μm, and 0.2 μm particles, respectively. Proliferation assays were performed as follows.

[0174] As stimulants, ovalbumin (SigmaAldrich) and BSA (SigmaAldrich) bound to Dynabeads® MyOne™ carboxylic acid particles were used. ich) was used (OVA particles or BSA particles). Mice were immunized with ovalbumin by monthly injection with 100 μg ovalbumin (Sigma) adsorbed to aluminum hydroxide. Three months after the first injection, mice were sacrificed and spleens were harvested. Splenocytes were prepared by standard procedures described in Thunberg et al. 2009, Allergy 64:919.

[0175] The cells were incubated with OVA or BSA particles (10 particles / cell) in cRPMI for 5 days. All cells were incubated at 37°C in a humidified atmosphere of 6% CO2 for 6 days. For the final 18 hours of incubation, 1 μCu / well [ 3 [H] thymidine was added to cell cultures. The mean counts per minute (cpm) from stimulated triplicates were divided by the mean cpm values ​​from unstimulated cells and expressed as the stimulation index (SI). SI values ​​≥ 2.0 are generally considered positive.

[0176] As can be seen in Figure 1, cells incubated with OVA particles with a diameter of 0.2 μm showed increased proliferation, with a mean SI of 4.1 (95% CI 2.4-5.8, P=0.007). Cells incubated with OVA particles with a diameter of 1 μm showed increased proliferation, with a mean SI of 8.4 (95% CI 6.1-10.6, P<0.005). Cells incubated with OVA particles with a diameter of 5.6 μm were unable to stimulate proliferation, with a mean SI of 1.1 (95% CI 0.4-2.7, P=0.876).

[0177] These results show that antigens bound to particles of different sizes can stimulate cell proliferation. Particles with a diameter of about 1 μm appear to be the most efficient in terms of cell stimulation, but particles up to a size of 0.2 μm still work. As the diameter approaches 5.6 μm, particles fail to fully stimulate cells, but it is reasonable to predict that particles with sizes larger than 1 μm will also work. It is reasonable to assume that 1 μm is the optimal size since it is similar to the size of bacteria. Our immune system has evolved to phagocytose and respond to microorganisms / particles of this size. Normal antigen-presenting cells have a size in the range of 10-15 μm.

[0178] Example 3b: Comparison of different sizes of antigen-bound particles and their efficacy in activating and expanding T cells (i) Preparation of antigen-bound phagocytizable particles Three paramagnetic polystyrene phagocytable particles of different sizes were used. - 1 μm diameter (Dynabeads MyOne Carboxylic Acid, ThermoFisher) - 2.8 μm diameter (Dynabeads M-270 Carboxylic Acid, ThermoFisher) - 4.5 μm diameter (Dynabeads M-450 epoxy, ThermoFisher)

[0179] The phagocytosis particles were conjugated with the model antigen cytomegalovirus (CMV) protein pp65 construct (SEQ ID NO: 19) according to the manufacturer's instructions. To remove endotoxin, the phagocytosis particles were washed five times with 0.75 M sodium hydroxide buffer, then resuspended in sterile PBS. (ii) Incubation of antigen-bound phagocytizable particles

[0180] Peripheral blood mononuclear cells (PBMCs) from CMV-susceptible healthy donors, isolated by standard Ficoll-based density gradient centrifugation, were incubated in 48-well plates at 37°C, 5% CO2, 500,000 cells / well for 18 h at 1,000,000 cells / ml with phagocytosis-competent particles conjugated to CMV constructs (hereafter referred to as "CMV particles"), and incubated at 1,000,000 cells / ml for 18 h at 37°C, 5% CO2, 500,000 cells / well ... The CMV particles were cultured at 100 μm, 2.8 μm, and 4.5 μm particle sizes. The CMV particle concentrations were normalized based on total surface area (a surrogate marker for the amount of CMV when bound to the surface of the CMV particle). This equates to 10 CMV particles / PBMC for 1 μm size particles, 1.4 CMV particles / PBMC for 2.8 μm size particles, and 0.5 CMV particles / PBMC for 4.5 μm size particles, based on the total number of PBMC in the sample. The results for each particle size are shown in Table 1 below. [Table 1]

[0181] (iii) Assessment of uptake After incubation, the number of phagocytosed CMV particles was counted manually using a confocal microscope. Eight cells were counted to obtain the mean and standard deviation values. Figure 5A shows a confocal microscope image of a representative cell with phagocytosed CMV particles within the cell. The black dashed line indicates the cell outline. The white line indicates the overall dimensions of the intracellular CMV particle.

[0182] This method was not applicable to 1 μm CMV particles because the particles were too small to be counted accurately. To assess the size of 1 μm CMV particles, the total volume of all phagocytosed CMV particles was measured and the size of individual CMV particles was back-calculated based on the total volume, assuming a packing density of 60%. Since this method proved to be fairly accurate for 2.8 μm CMV particles (estimated 11.9 CMV particles / cell compared to 9.1 CMV particles / cell counted manually) and 4.5 μm CMV particles (estimated 2.4 CMV particles / cell compared to 3.1 CMV particles / cell counted manually), it can be assumed that it will also accurately estimate the size of 1 μm CMV particles.

[0183] The uptake of CMV particles is shown in Figures 5B and 5C. Figure 5B shows the number of CMV particles taken up by each cell as assessed by manual counting (8 cells were counted per bead type). Using the manual counting method, the number of phagocytosed particles per cell for 4.5 μm CMV particles was found to be 3.1 (± 1.1). For 2.8 μm CMV particles, it was 9.1 (± 2.2). It was not possible to count the number of 1 μm CMV particles using this method.

[0184] Figure 5C shows the number of CMV particles ingested by each cell (three cells measured per bead type) as assessed by volumetric calculation (*p<0.05 **p<0.01 *** p<0.001, calculated using Student's T-test). Using the volumetric calculation method, the number of phagocytosed particles per cell for 4.5 μm CMV particles was found to be 2.4 (±1.1); for 2.8 μm CMV particles, it was 11.9 (±3.2); and for 1 μm CMV particles, it was 203.7 (±21.9).

[0185] Based on the number of CMV particles ingested by each cell, assessed by volumetric calculations, the total surface area of ​​phagocytosed CMV and, therefore, the total amount of CMV was calculated. The surface area ingested was 639.6 (±68.9) μm for a 1 μm CMV particle. 2 , 293.1 (±79.3) μm for 2.8 μm CMV particles 2 , and 150.7 (±67.0) μm for 4.5 μm CMV particles. 2 These data are shown in Table 2 below. [Table 2]

[0186] (iv) Assessment of T cell stimulation The ability of antigen-coupled particles to stimulate T cells to promote their proliferation was assessed by measuring the release of IFNγ, IL22 and IL17A from PBMCs using a FluoroSpot assay (Mabtech, Sweden). PBMCs (250,000 / well) from CMV-susceptible healthy donors (n=2) were stimulated with CMV particles in triplicate. The concentration of antigen-coupled particles was normalized as described above based on total surface area (10×1 μm CMV particles / cell, 1.4×2.8 μm CMV particles / PBMC, and 0.5×4.5 μm CMV particles / cell). The number of PBMCs per well in the FluoroSpot assay, together with the estimated number of monocytes per well (based on the assumption that monocytes make up 20% of the content of the PBMC sample), is shown below for each particle size (Table 3). [Table 3]

[0187] PBMCs were incubated at 37°C, 5% CO2 for 44 hours. Plates were developed according to the manufacturer's instructions and read on an automated FluoroSpot reader. Data reported for FluoroSpot are the number of spots when cells were stimulated with CMV particles, which is greater than the number of spots when cells were not stimulated with CMV particles.

[0188] The levels of IFNγ production, as assessed by FluoroSpot assay, are shown in Figure 6A and show little difference between the CMV particles.

[0189] The levels of IL22 and IL17 production assessed by FluoroSpot assay are shown in Figures 6B and 6C. It can be seen that 1 μm CMV particles induced significantly higher IL22 and IL17 production in one individual than larger CMV particles, with a similar trend seen in the other individual for IL22.

[0190] The levels of dual cytokine production assessed by FluoroSpot assay are shown in Figures 6D and 6E. It can be seen that 1 μm CMV particles induced significantly higher dual cytokine release (IFNγ+IL17 and IL22γ+IL17) than larger CMV particles in one healthy donor when stimulated with 1 μm antigen-coupled particles.

[0191] Cytokine release in these experiments is a surrogate for T cell proliferation. In general, IFNγ is produced by CD4+ T cells (Th1 subclass) and CD8+ T cells. IL17 and IL22 are produced primarily by proinflammatory Th17 CD4+ T cells. Such cells have been shown to be proinflammatory and aid in tumor eradication. The data provide further evidence that 1 μm beads activate and proliferate Th1 CD4+ and CD8+ T cells to the same extent as other beads, as well as proinflammatory Th17 CD4+ T cells and, to a lesser extent, dual cytokine-producing T cells that are also proinflammatory. This suggests that there are benefits.

[0192] Example 4: Preventive effect of phagocytosis-competent particles in mouse cancer models In this experiment, two groups of phagocytosis particles were used: 1) phagocytosis particles comprising a polystyrene particle core and the neoantigenic construct M272120 (SEQ ID NO: 1) tightly associated with the core, and 2) phagocytosis particles comprising a polystyrene particle core and the neoantigenic construct M304748 (SEQ ID NO: 2) tightly associated with the core. The phagocytosis particles were prepared using the methods described herein in Examples 1 and 2. That is, the neoantigenic constructs were coupled to 1 μm superparamagnetic beads (Sera-Mag SpeedBeads (hydrophilic) carboxylate-modified magnetic particles, GE Healthcare) according to the protocols outlined in Examples 1 and 2. The neoantigenic constructs were designed based on previously published studies using the B16-F10 tumor model (Kreiter et al. (2015), Nature, 520:692 and Castle et al. (2012), Cancer Res, 72:1081). After preparation, the two groups of phagocytizable particles were mixed (1:1 ratio) and then purified to prepare a stock solution of the particles in PBS containing CpG oligodeoxynucleotide (ODN 1668, Enzo) as an adjuvant, which had a concentration of 10 million particles / μl.

[0193] Healthy mice (C57 BL / 6 mice) were administered low doses (1 μl of phagocytizable particles in 9 μl of PBS) or high doses (10 μl of phagocytizable particles) of phagocytizable particles by injection into either the inguinal lymph node or subcutaneously. Each dose and route of administration was evaluated in triplicate (n=3). The stock phagocytizable particle samples used to prepare the low and high doses contained 10 million beads / μl. Thus, the low dose contained approximately 10 million phagocytizable particles and the high dose contained approximately 100 million phagocytizable particles. Each dose of phagocytosis particles contained two different groups of phagocytosis particles: 1) phagocytosis particles comprising a polystyrene particle core and having the neoantigenic construct M272120 (SEQ ID NO: 1) tightly associated with the core, and 2) phagocytosis particles comprising a polystyrene particle core and the neoantigenic construct M304748 (SEQ ID NO: 2) tightly associated with the core.

[0194] Mice were administered a first dose of phagocytizable particles on day 1, and then a second dose was administered to the same mice about one month later (day 33). Blood samples were taken from the mice about 3 weeks (day 22) after the first administration of phagocytizable particles and about 3 weeks (day 23) after the second administration of phagocytizable particles.

[0195] The health status of mice that received two doses of phagocytosis particles by inguinal lymph node injection was also evaluated.We found that 2×10μl injection of phagocytosis particles via inguinal lymph node did not affect animal health status (body weight and overall health).Inguinal lymph node and mouse spleen also did not show gross abnormality, suggesting that phagocytosis particles were well tolerated by mice.

[0196] The collected blood samples were analyzed using an enzyme-linked immunosorbent assay (ELISA). The assay was performed in 96-well plates. The plates were coated with neoantigenic constructs M272120 (SEQ ID NO: 1) or M304748 (SEQ ID NO: 2) by adding 100 μl of neoantigenic constructs at a concentration of 5 μg / ml in PBS. The plates were incubated overnight and then washed. The coated 96-well plates were then incubated with 100 μl of diluted (1:1000) mouse serum taken from mice after administration of the first or second dose of phagocytizable particles. The plates were then washed and subsequently incubated with anti-mouse IgG-HRP secondary antibody (Jackson Labs, 1:8000 dilution). Finally, the plates were washed and then incubated with TMB substrate solution (Sigma-Aldrich ) and absorbance was measured at 370 and 650 nm. The absorbance of each mouse serum sample for each neoantigenic construct is shown in Figure 7A (serum collected from mice after 1st dose, neoantigenic construct = M272120 (SEQ ID NO: 1)), Figure B (serum collected from mice after 1st dose, neoantigenic construct = M304748 (SEQ ID NO: 2)), Figure 7C (serum collected from mice after 2nd dose, neoantigenic construct = M272120 (SEQ ID NO: 1)), and Figure 7D (serum collected from mice after 2nd dose, neoantigenic construct = M304748 (SEQ ID NO: 2)). As a control sample, serum samples collected from naive mice (n = 3, no dose of phagocytizable particles administered) were also analyzed. As shown by Figures 7A, 7B, 7C, and 7D, mice that received a high dose of phagocytizable particles (administered intranodally or subcutaneously) had a higher percentage of anti-neoepitope antibodies in their serum samples compared to serum samples taken from mice that received a low dose of phagocytizable particles via the same route and from mice that did not receive phagocytizable particles (i.e., naive mice).

[0197] Approximately 2 months (54 days) after the administration of the second dose of phagocytosis-capable particles, mice were subcutaneously injected with 500,000 cells of melanoma cancer cell line B16-F10. Tumor volume was measured daily. Figure 8 shows the increase in tumor volume over time (days, D).

[0198] Example 5: Pilot study utilizing phagocytosis-competent particles for ex vivo expansion of anti-cancer T cells (i) Identification of neoepitope targets in bladder cancer Urinary bladder cancer exhibits a high mutation rate and, as a result, expresses numerous neoepitopes that may be recognized as non-self by the immune system. Therefore, we investigated tumor polymorphisms suitable as neoepitope peptides and T cell targets by searching a mutation database that contains a large repository of potential neoepitopes that can be used to expand T cells for immunotherapy.

[0199] The COSMIC database contains mutation data of 4754 transitional cell carcinomas from both whole exome analysis and hotspot analysis. We focused on bladder cancer (UBC) and selected the 15 most common mutations that lead to single amino acid mutations, especially substitution mutations, and therefore qualified as neoepitopes. We also focused on polymorphisms in genes known to be related to tumor pathogenesis, such as kinases, growth factor receptors, and cell cycle proteins. The selected 15 mutations alone cover 73% of bladder cancer mutations found in COSMIC. The neoepitope peptides identified from the COSMIC database are referred to as "predicted neoepitope peptides" in this example.

[0200] Alternatively, whole genome sequencing of tumors from UBC patients is performed to identify additional polymorphisms and new targets for immunotherapy. Tumor RNA sequencing can be performed to confirm the presence of polymorphic transcripts. Using multiple reaction monitoring (MRM) mass spectrometry transitions, patients can be rapidly scanned for the expression of the most common neoepitopes at the protein level, and the neoepitope peptides used for individual immunotherapy can be tailored. The neoepitope peptides identified using this method are referred to as "individualized neoepitopes" in this example.

[0201] The neoantigenic construct was designed as a 21 amino acid peptide with a somatically mutated amino acid at one amino acid position in the middle of the sequence (i.e., with a somatically mutated amino acid at amino acid position 11). To design the neoantigenic construct, the three neoepitope peptides were linked with two VVR spacers because the VVR motif is post-translationally cleaved by cathepsin S in the lysosome as a step in human leukocyte antigen presentation. (iii) Neoepitope-mediated T cell activation and proliferation

[0202] As described above, nine neoepitopes were bioinformatically identified. The predicted neoepitope peptides were based on genes with reported bladder cancer-associated mutations, such as FGFR3 and p53. In a pilot study using a neoantigenic construct containing three neoepitope peptides, we were able to identify IFN-γ-producing T cells from the blood of patients with bladder cancer by FluoroSpot, demonstrating the validity of the neoepitope peptide approach.

[0203] For the same patient with bladder cancer, activation of T cells was performed with the predicted neoepitope peptides NA1-9 (SEQ ID NOs: 4-12, see Table 4 below). Proliferation was observed in response to NA1, 3, 5, 7 and 8 (SEQ ID NOs: 4, 6, 8, 10 and 11). Figure 2A shows the number of cells in PBMC cultures over time (PB = peripheral blood) after incubation with APCs contacted with phagocytizable particles containing the predicted neoepitope peptides (NA1-9) bound to polystyrene particles. The arrows in Figure 2A indicate the time of restimulation (i.e., the time when the T cell sample was contacted with a second batch of APCs contacted with phagocytizable particles containing the predicted neoepitope peptides (NA1-9) under conditions that allow specific activation of anti-cancer T cells in response to the neoepitopes presented by the APCs). Figure 2B shows the % of CD4+ T cells / total T cells. FIG. 2C shows T-bet expression in CD4, and FIGS. 2D and E show granzyme B and perforin expression in CD8+ T cells.

[0204] Expanded T cells express the transcription factor T-bet and high levels of the effector molecules perforin and granzyme B (GZB).

[0205] The method of the invention has also been used on cells from a patient with disseminated colon cancer for which sequenced tumor data was available. The patient presented with two polymorphisms in p53, one of which was known and the other novel. The patient also presented with a mutation in PIK3CA. A personalized neoantigenic construct, comprising three neoantigenic constructs containing three neoepitope peptides and having an amino acid sequence according to SEQ ID NO: 3, was designed, expressed and purified based on specific mutations in the patient's tumor data, allowing the identification of personalized neoepitopes. Phagocytosis-capable particles were prepared by coupling the neoantigenic constructs to polystyrene particles according to the protocols outlined in Examples 1 and 2. Phagocytosis-capable particles containing personalized neoantigenic constructs (SEQ ID NO: 3) were used to grow cells, resulting in a neoepitope-specific response. Peripheral blood mononuclear cells (PBMCs) were used for the culture. FIG. 3 shows CD4+ cells (circles) expanding in T samples before and during expansion with phagocytosable particles comprising a polystyrene particle core and an individualized neoantigenic construct tightly associated with the core, along with the percentage of T cells (small squares) and the total number of CD4+ T cells (large squares).

[0206] FIG. 4A shows the number of cells (days) over time in PBMC cultures. The top line (Pat2 personalized NA) shows the number of cells in PBMC cultures after incubation contacted with phagocytosis particles containing personalized neoantigenic constructs (SEQ ID NO: 3). The bottom two lines (Pat2 NA1+3 and Pat2 NA4+5) show the number of cells in parallel PBMC cultures contacted with phagocytosis particles of NA1 and NA3 or with phagocytosis particles of NA4 and NA5. FIG. 4B shows the % of CD4+ T cells / total T cells. The top line in FIG. 4B at 14 days and later is for the experiment of personalized NA of Pat2. The arrows in FIG. 4A and FIG. 4B show the time of restimulation (i.e., the time when the T cell sample was contacted with a second batch of APCs contacted with phagocytosis particles under conditions that allow specific activation of anti-cancer T cells in response to neoepitopes presented by the APCs). FIG. 4C visualizes the analysis performed using the Barnes-Hut Stochastic Neighbor Embedding (BH-SNE) algorithm for CD4+ T cells, where all cells in the sample were selected The tumors are clustered on a two-dimensional map according to similarity in expression intensity by a set of markers, here CD28, CD57, T-bet, GATA-3, perforin, granzyme B (GZB), Ki-67 and PD-1.

[0207] Expression of the proliferation marker Ki67 and the number of T cells increased during proliferation, and expression of key markers for antitumor activity such as T-bet, perforin, and granzyme B increased in both CD4+ and CD8+ cells over the 14-day culture period. The percentage of CD8+ T cells decreased to approximately 10% of CD4+ T cells, but the total number of CD8+ cells increased.

[0208] The entire process from receiving sequence data to analysis of proliferated cells can be completed in 4–5 weeks.

[0209] These results indicate that there was a neoepitope-specific T cell response. As described above, the inventors have demonstrated that predicted and personalized neoepitope peptides can be designed and used for T cell activation and proliferation.

[0210] The methods of expanding T cells of this example, and T cells expanded using the expansion methods of this example, find utility in the uses and methods for treating and preventing cancer described herein. [Table 4]

[0211] Example 6: Tumor mouse model to evaluate tumor growth following vaccination with phagocytizable particles coupled to MC38 colorectal tumor-specific neoantigen

[0212] Materials and Methods A phagocytosis particle composition was used in this study (referred to herein as the "MC38 particle composition") that contained six different phagocytosis particle populations. Each particle population contained a polystyrene particle core (Sera-Mag SpeedBeads (hydrophilic) carboxylate-modified magnetic particles, GE Healthcare) coupled to one of the following types of MC38 neoantigen constructs: 1) SEQ ID NO:13, 2) SEQ ID NO:14, 3) SEQ ID NO:15, 4) SEQ ID NO:16, 5) SEQ ID NO:17, 6) SEQ ID NO:18 (see Table 5). [Table 5]

[0213] Each MC38 neoantigenic construct contains six different 20-23 amino acid neoantigenic peptide sequences derived from the MC38 colon cancer cell line. The MC38 neoantigenic constructs were recombinantly expressed using E. coli and purified using column chromatography.

[0214] MC38 particles were prepared according to the protocol outlined in Example 1. After production, the six groups of phagocytizable particles were mixed and then sterilized using the protocol described in Example 2. A stock solution of the MC38 particle composition was prepared at a concentration of 10 million particles / μl in PBS.

[0215] Test mice (n=5) were administered the first and second doses of MC38 particle composition. The total volume of each dose was 10 μl (9.5 μl of MC38 particle composition stock solution, 0.5 μl of 0.05 nmol CpG oligodeoxynucleotide (ODN 1668, Enzo) as adjuvant). Control mice were not treated (n=5, non-vaccinated group).

[0216] The first dose of phagocytosis-capable particles (MC38 particle composition) was injected 5 days before implantation of MC38 tumor cells (time point A in FIG. 9). MC38 tumor cells were implanted on day 0 (time point B in FIG. 9). 6 Engraftment was achieved by injecting each mouse with MC38 tumor cells. Thirteen days after engraftment, the mice were injected with a second dose (time point C in FIG. 9) of the same type of phagocytizable particles (i.e., MC38 particles) as the first dose. The tumor volume of each mouse was measured over the course of the study (see FIG. 9).

[0217] result The results are shown in Figure 9. As can be seen from Figure 9, tumor growth was significantly reduced in mice administered MC38 particles ("vaccinated" mice) compared to non-vaccinated mice ("negative control" mice). These results indicate that MC38 particles significantly inhibit the growth of MC38 colorectal tumors. It has been shown to be effective in inducing an anti-cancer immune response that inhibits tumor growth in mouse models.

[0218] Example 7: Toxicity and biodistribution studies To assess the maximum tolerated dose of phagocytizable particles, a rat model is used to evaluate the toxicity and biodistribution profile of the phagocytizable particle core.

[0219] Materials and Methods The particles used in the tests are Sera-Mag SpeedBead carboxylate-modified magnetic particles (hydrophilic). These particles are supplied by GE Healthcare Life Sciences (particle lot / batch number GE:16807675, concentration: 2.3% solids (g / 100 g) in Dulbecco's phosphate buffered saline (equivalent to 10 mg Fe / mL), pH 7.1). The particles are stored at 2-8 °C before use.

[0220] The animals are specifically Wistar rats. Upon arrival at the testing site, the rats weigh approximately 250 g. The rats are allowed to acclimate for a minimum of 5 days before the start of the test. The rats are kept in individually ventilated cages (IVC type 4) at +22°C ± 3°C, humidity 50% ± 20%, 12 h light / 12 h dark cycle. Food and water are available ad libitum. There are 3 rats per cage.

[0221] Test 1) Five female Wistar rats are used for the pilot study. Rat #1 is injected intravenously (IV) with the particles at the maximum concentration feasible (5 mL / kg, equivalent to 50 mg / kg iron), after which the rat is placed in a computed tomography (CT) camera for image acquisition. The intravenous injection is performed as slowly as possible. If a toxic response is evident, the particles are delivered to the remaining rats by slow infusion over 20 minutes (maximum 20 mL / kg). The particle concentration delivered to the rats is titrated until the maximum tolerated dose is identified. Rats not receiving a dose of particles are used as negative controls to acquire background CT scans.

[0222] After IV injection / infusion of particles, rats are anesthetized with isoflurane and ophthalmic ointment is placed on the eyes to prevent dehydration. The rats are then placed on the heated bed of the CT machine to maintain inhalation anesthesia. Vital parameters (pulse and respiration) are monitored during the course of the experiment using a respiratory sensor and a rectal thermometer. The rats are placed in the CT camera and pictures are acquired for approximately 20 minutes. Health status after IV injection of particles is recorded to evaluate possible toxic reactions and whole-body CT images are used to evaluate particle visibility and to identify particle location after injection. After acquisition of CT images, the rats are euthanized.

[0223] Test 2) Further testing will be performed with 12 rats to identify particle clearance rates. All rats will receive an IV injection of particles at the dose identified in the pilot study. Immediately after particle administration, the rats will be placed in the CT camera. The rats will then be monitored with the CT camera 24 hours, 3 days, 7 days, 14 days, and 1 month after administration. At each time point, two rats will be euthanized to remove organs for histopathological analysis. The rats will be monitored for health and weight changes 24 hours, 3 days, and 7 days after administration, and weekly thereafter.

[0224] Example 7: Illustrative Phagocytizable Particle Sterilization Protocol Using Bacillus subtilis The experiments were carried out under sterile conditions in a laminar airflow (LAF) safety cabinet. Phagocytizable particles containing cores (Sera-Mag SpeedBeads carboxylate-modified magnetic particles, GE Healthcare) bound to the original construct were washed four times with high-concentration alkaline solution (2M to 5M NaOH). After the first wash, the phagocytizable particles were transferred to a new sterile tube, the supernatant was removed, and a second amount of alkaline solution was added. The phagocytizable particles were sonicated in a sonication bath for 10 min and then incubated in the same alkaline solution for 30 min with swirling motion. This process was repeated two more times, followed by four washes with sterile Dulbecco's modified PBS.

[0225] The effectiveness of the NaOH treatment protocol was evaluated by adding a high load (>1.2 CFU) of bacillus subtilis subsp. spizizenii (ATCC® 6633™ Epower 106 CFU) to phagocytosable particles (Sera-Mag SpeedBeads carboxylate-modified magnetic particles without attached neoantigens) followed by the NaOH treatment protocol described above. After NaOH treatment, both the complete bead suspension and supernatant from 5M or 2M NaOH-treated samples versus untreated samples (positive control) were plated on nutrient agar without antibiotics and incubated overnight (>16 hours) at 37°C.

[0226] result While no washing treatment resulted in abundant growth of Bacillus subtilis subsp. spizizenii colonies, both 5M and 2M NaOH treatments effectively neutralized bacterial growth. In conclusion, both 2M and 5M NaOH treatments were highly effective in removing artificially elevated bioburden from phagocytizable particles.

Claims

1. A phagocytosis particle for use in treating or preventing cancer in a subject, comprising a core and a neoantigenic construct tightly associated with the core, said neoantigenic construct comprising a neoepitope peptide having an amino acid sequence corresponding to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by cancer cells of the subject, said portion of the protein or peptide having at least one somatically mutated amino acid.

2. The phagocytizable particle for use according to claim 1 , wherein the neoantigenic construct comprises two or more covalently linked neoepitope peptides.

3. The phagocytizable particle for use according to claim 2, wherein the neoantigenic construct comprises three or more covalently linked neoepitope peptides, for example three, four or five covalently linked neoepitope peptides.

4. The phagocytizable particle for use according to claim 2 or 3, wherein the covalently attached neoepitope peptide is covalently attached via a spacer moiety.

5. The phagocyte-able particle for use according to claim 4, wherein the spacer portion is a sequence of 1 to 15 amino acids, preferably a sequence of 1 to 5 amino acids, more preferably comprising the amino acid sequence VVR and / or the amino acid sequence GGS.

6. A phagocytizable particle for use according to any one of claims 2 to 5, wherein each of the covalently attached neoepitope peptides is between 3 and 25 amino acids in length.

7. A phagocytizable particle for use according to any one of claims 1 to 6, wherein the neoantigenic construct is covalently attached to the core.

8. A phagocytizable particle for use according to any one of claims 1 to 7, wherein the phagocytizable particle comprises two or more different neoantigenic constructs tightly associated with the core, for example comprising two, three, four or five neoantigenic constructs tightly associated with the core.

9. The phagocytizable particle for use according to claim 8 , wherein each of the different neoantigen constructs comprises a different neoepitope peptide sequence or a different combination of neoepitope peptides.

10. 10. A phagocytizable particle for use according to any one of claims 1 to 9, wherein the phagocytizable particle has a maximum dimension of less than 5.6 μm, preferably less than 4 μm, more preferably less than 3 μm, even more preferably between 0.5 and 2 μm, or most preferably about 1 μm.

11. A phagocyte-containing particle for use according to any one of claims 1 to 10, wherein the core is paramagnetic or superparamagnetic.

12. 12. The phagocyte-containing particle for use according to any one of claims 1 to 11, wherein the core comprises a polymer, preferably polystyrene.

13. The phagocytizable particles are administered with an adjuvant or contain an adjuvant (e.g., IL-2, IL-15, IL-17, and IL-4) tightly associated with the core. A phagocyte-containing particle for use according to any one of claims 1 to 12.

14. An injectable pharmaceutical composition comprising a phagocytosis particle comprising a core and a neoantigenic construct tightly associated with the core, wherein the neoantigenic construct comprises a neoepitope peptide having an amino acid sequence corresponding to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by cancer cells in the subject, the portion of the protein or peptide having at least one somatically mutated amino acid.

15. 15. The injectable pharmaceutical composition of claim 14, wherein the phagocytizable particles are defined in any one of claims 2 to 13.

16. 16. An injectable pharmaceutical composition according to claim 14 or 15 for use in the treatment or prevention of cancer in a subject.

17. The phagocytizable particles for use according to claims 1 to 13 or the injectable pharmaceutical composition for use according to claim 16, wherein the cancer is a solid cancer, such as a cancer selected from breast cancer, colon cancer, liver cancer, lung cancer (non-small cell and small cell), lung carcinoid tumor, pancreatic cancer, prostate cancer, ovarian cancer and bladder cancer.

18. A method for treating or preventing cancer in a subject, comprising administering to the subject a phagocytosis-capable particle, the phagocytosis-capable particle comprising a core and a neoantigenic construct tightly associated with the core, the neoantigenic construct comprising a neoepitope peptide having an amino acid sequence corresponding to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by cancer cells in the subject, the portion of the protein or peptide having at least one somatically mutated amino acid.

19. The treatment or prevention of cancer further comprises: The method of any one of claims 1 to 13, or the method of claim 16, comprising administering one or more subsequent doses of the phagocytizable particle or injectable pharmaceutical composition to the subject, the subject having previously been administered a dose of the phagocytizable particle or injectable pharmaceutical composition sufficient to induce an immune response against cancer cells in the subject.

20. The treating or preventing cancer in the subject further comprises: a) administering the phagocytosis-capable particles to the subject, and then harvesting APCs and anti-cancer T cells from the subject; b) expanding the anti-cancer T cells obtained from the subject; and c) administering to said subject a therapeutic dose of said expanded anti-cancer T cells.

21. The phagocytizable particle or injectable pharmaceutical composition for use according to claim 20, wherein the APCs and anti-cancer T cells are obtained from a PBMC sample derived from the subject, e.g. the APCs and anti-cancer T cells are obtained from the same PBMC sample or the APCs and anti-cancer T cells are obtained from different PBMC samples.

22. The step b) of activating and expanding anti-cancer T cells comprises: i. providing a phagocytizable particle comprising a core and a neoantigenic construct tightly associated with said core, said neoantigenic construct inhibiting the proliferation and proliferation of cancer cells in said subject; a neoepitope peptide having an amino acid sequence corresponding to the amino acid sequence of a portion of a protein or peptide known or suspected to be expressed by a cell, said portion of said protein or peptide having at least one somatically mutated amino acid; ii. providing APCs; iii. contacting the phagocytosis-capable particle with the APC in vitro under conditions that allow phagocytosis of the phagocytosis-capable particle by the APC; iv. Providing anti-cancer T cells harvested from the subject; v. contacting in vitro the anti-cancer T cells with the APCs from step iii) under conditions that allow specific activation of the anti-cancer T cells in response to the neo-epitopes presented by the APCs.

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