Topical skin preparation

Abstract

To provide an active ingredient of a topical skin preparation for preventing cellular senescence that is sourced from natural substances and exhibits high biological safety.SOLUTION: A topical skin preparation for preventing cellular senescence contains a fermented product from plants belonging to the genus Hibiscus of the Malvaceae family and an extract from plants of the genus Rosa in the Rosaceae family as active ingredients.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to an external skin preparation for preventing cellular aging, which contains as active ingredients a combination of a fermented product of a plant belonging to the genus Hibiscus in the family Malvaceae and an extract of a plant belonging to the genus Rosa in the family Rosaceae. [Background technology]

[0002] In recent years, research on cellular aging has been conducted, and it is known that aged cells secrete factors (inflammatory cytokines, etc.) that induce aging in surrounding cells. These are called senescence-associated secretory phenotypes (SASPs), and are known to induce aging in surrounding cells. For example, cellular aging is thought to be involved in the decrease in collagen synthesis in fibroblasts in the dermis, activating collagenase and decomposing the extracellular matrix, leading to wrinkles, sagging, etc. For this reason, there is a demand in the field of topical skin preparations for ingredients that suppress the expression of SASP factors and inhibit cellular aging. DISCLOSURE OF THEINVENTION [Problem to be solved by the invention]

[0003] In view of the problems with the conventional technology, the inventors have discovered that a combination of a fermented product of a plant belonging to the genus Hibiscus in the family Malvaceae and an extract of a plant belonging to the genus Rosaceae in the family Rosaceae suppresses the gene expression of an inflammatory factor known as a SASP factor (interleukin-1α [IL-1α]) and matrix metalloproteinase-3 [MMP3], which is secreted from fibroblasts, synovial cells, and chondrocytes and breaks down collagen.

[0004] Conventionally, topical skin preparations containing as an active ingredient a fermented product of a plant belonging to the genus Hibiscus in the family Malvaceae are known, for example, from Patent Document 1, and topical skin preparations containing as an active ingredient an extract of rose belonging to the genus Rosaceae in the family Rosaceae are known, for example, from Patent Documents 2 to 6.

[0005] [Patent Document 1] Patent Publication No. 2006-347925 [Patent Document 2] Patent Publication No. 2000-327555 [Patent Document 3] JP2001-064192A [Patent Document 4] JP 2001-163794 A [Patent Document 5] Patent Publication No. 2001-316277 [Patent Document 6] Patent Publication No. 2014-240375

[0006] However, it was not known that a combination of a fermented product of a plant belonging to the genus Hibiscus in the family Malvaceae and an extract of a plant belonging to the genus Rosaceae in the family Rosaceae has the effect of suppressing the expression of SASP factors. [Means for solving the problem]

[0007] The present invention relates to an external skin preparation for preventing cellular aging, which contains a fermented product of a plant belonging to the genus Hibiscus in the family Malvaceae and an extract of a plant of the genus Rosaceae in the family Rosaceae. Effect of the Invention

[0008] The present invention provides an external skin preparation that suppresses the expression of SASP factors and inhibits cellular aging by using as active ingredients a combination of a fermented product of a plant belonging to the genus Hibiscus in the family Malvaceae and an extract of a plant of the genus Rosaceae. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0009] Preferred embodiments of the present invention will now be described in detail. First, the fermented product of a plant belonging to the genus Hibiscus of the family Malvaceae will be described. Examples of plants belonging to the genus Hibiscus of the family Malvaceae include roselle (Hibiscus sabdariffa L.), rose of Sharon (Hibiscus syriacus), Hibiscus mutabills, Hibiscus coccineus, Hibiscus tiliaceus, Hibiscus rosa-sinensis, and Hibiscus schizopetalus. There is no particular limitation on the part of a plant belonging to the genus Hibiscus that can be used, and any part of the plant, such as the whole plant, leaves, stems, flowers, calyx, stamens, pistils, stems, roots, seeds, and fruits, can be used, but it is preferable to use the whole plant, flowers, and calyx.

[0010] As a source of assimilation for fermentation of a plant of the genus Hibiscus, the plant itself (hereinafter referred to as the plant body) may be used, or an extract obtained from the plant body by a solvent extraction method described below may be used. When an extract is used, it is also possible to carry out fermentation while still including the plant body, without removing the plant body to be extracted by solid-liquid separation. Here, the plant may be fresh, or may be previously dried or semi-dried. In terms of form, it is also possible to use the plant as it is after it has been harvested.

[0011] In the present invention, examples of microorganisms used in the fermentation of plants of the genus Hibiscus include lactic acid bacteria, bifidobacteria, koji mold, natto bacteria, tempeh bacteria, yeast, etc., and generally one or more species selected from each of these species are used, but in some cases, or as long as they do not interfere with each other's fermentation, different species of bacteria may be used in combination.

[0012] For example, examples of lactic acid bacteria include lactic acid bacteria of the genus Lactobacillus, such as Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, and Lactobacillus delbrueckii; lactic acid bacteria of the genus Carnobacterium, such as Carnobacterium divergens and Carnobacterium piscicola; lactic acid bacteria of the genus Leuconostoc, such as Leuconostoc mesenteroides and Leuconostoc citreum; lactic acid bacteria of the genus Leuconostoc, such as Streptococcus faecalis; lactic acid bacteria of the genus Streptococcus such as Streptococcus pyogenes, Enterococcus caseliflavus, Enterococcus sulfreus, Lactococcus plantarum, Lactococcus rafinolactis, Veissella confusa, Veissella kandleri, Atopobium minutum, Atopobium Lactic acid bacteria of the genus Atopobium, such as Atopobium parvulus; lactic acid bacteria of the genus Vagococcus, such as Vagococcus fluvialis and Vagococcus salmoninarum;Examples of such lactic acid bacteria include lactic acid bacteria of the genus Pediococcus, such as Pediococcus damnosus and Pediococcus pentosaceus, and marine lactic acid bacteria, such as Marinilactobacillus phychrotolerans.

[0013] For example, bifidobacteria include Bifidobacterium bifidum and Bifidobacterium breve, and any of them can be used as long as they are classified as bifidobacteria.

[0014] Examples of koji mold include yellow koji molds such as Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, and Aspergillus sojae; black koji molds such as Aspergillus awamori, Aspergillus kawauchii, Aspergillus usami, and Aspergillus niger; and red koji molds such as Monascus anka and Monascus pilosus.

[0015] Examples of natto bacteria include bacteria of the genus Bacillus, such as Bacillus natto, Bacillus subtilis, and Bacillus circulans. Among them, Bacillus natto is the most preferred because it is widely used in foods and is highly safe.

[0016] Examples of tempeh fungi include fungi (molds) of the genus Rhizopus, such as Rhizopus azygosporus, Rhizopus microsporus chinensis, Rhizopus microsporus oligosporus, Rhizopus niveus, and Rhizopus oryzae.

[0017] Examples of yeast include yeasts of the genus Saccharomyces such as Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, and Saccharomyces bayonus; yeasts of the genus Torulaspora such as Torulaspora delbruekii, Torulaspora fermentati, and Torulaspora rosei; yeasts of the genus Torulaspora such as Zygosaccharomyces rouxii and Zygosaccharomyces sojae; Yeasts of the genus Zygosaccharomyces, such as Zygosaccharomyces sake, Zygosaccharomyces miso, and Zygosaccharomyces lactis; yeasts of the genus Candida, such as Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake, and Candida scottii; yeasts of the genus Candida, such as Aureobasidium Pullulans, Aureobasidium mansonii, and Aureobasidium microstictum; Examples include yeasts of the genus Aureobasidium such as Aureobasidium microstictum.Of the above-mentioned yeasts, Saccharomyces cerevisiae is preferred from the viewpoints of safety and effectiveness, but any Saccharomyces cerevisiae of any origin can be used, including those derived from plants such as sake, lily, and cherry blossom flowers, and those of marine origin.

[0018] When the above-mentioned suspension or extract is fermented by a microorganism, sterilization is performed before the fermentation step to remove unwanted bacteria that may hinder fermentation. As a method for sterilizing and removing unwanted bacteria, a method may be used in which the fermentation material is washed in advance with sterilizing ethanol or the like and then suspended in a sterile solvent such as sterile water, or the fermentation material may be suspended in a solvent and then sterilized by heat sterilization or the like. As the heat sterilization treatment, a heat sterilization method such as an autoclave sterilization method in which the suspension is heated at 120 to 130°C for 10 to 20 minutes or an intermittent sterilization method in which the suspension is kept at 80 to 90°C for 60 to 120 minutes and this is repeated once a day for 2 to 3 days is generally used.

[0019] The sterilized suspension is placed in a fermentation tank and inoculated with microorganisms for fermentation. The inoculum size is 10 7 ~10 8 If the inoculum amount is more than the above range, the fermentation time will not change much, while if it is less than the above range, it will take a long time to complete the fermentation, which is not preferable.

[0020] A preferred example of the method for fermenting the above-mentioned plants using the above-mentioned microorganisms is as follows. First, the fermentation material of the plant is immersed or suspended in a fermentation medium to prepare a suspension for fermentation. In this case, the plant may be used raw or may be dried or semi-dried in advance. As for the form, the plant may be used as it is after harvesting, but the fermentation efficiency can be increased by shredding or crushing it into fine particles.

[0021] As the fermentation medium for suspending the fermentation material, water or a mixture of water with lower alcohols (methanol, ethanol, propanol, etc.) or glycols (ethylene glycol, propylene glycol (propanediol), 1,3-butylene glycol, glycerin, etc.) can be used. Sugars such as glucose, fructose, sucrose, etc. can also be added to these media. However, it is most preferable to use water alone, as this is the medium in which microorganisms are most likely to exert their functions and to avoid the production of fermentation by-products due to the presence of nutrient substances other than the plant material that is the fermentation material.

[0022] Before the suspension of the fermentation material is subjected to the fermentation process, it is sterilized to remove unwanted bacteria that may hinder fermentation. In this case, the sterilization and removal method may be a method in which the fermentation material is washed and sterilized in advance with sterilizing ethanol or the like, and then suspended in a sterile medium such as sterile water, or a method in which the suspension is heat sterilized after suspending the fermentation material in a medium. As the heat sterilization method, an autoclave sterilization method in which the suspension is heated at 120 to 130°C for 10 to 20 minutes, or an intermittent sterilization method in which the suspension is kept at 80 to 90°C for 60 to 120 minutes and this is repeated once a day for 2 to 3 days, is generally used.

[0023] Next, the sterilized suspension is placed in a fermentation tank and inoculated with microorganisms to carry out the fermentation process. 7 ~10 8 If the inoculum amount is more than the above range, the fermentation time will not change much, while if it is less than the above range, it will take a long time to complete the fermentation, which is not preferable.

[0024] The fermentation temperature is generally in the range of 5 to 50° C. The fermentation period is generally 1 to 10 days, preferably 2 to 5 days, at the optimum temperature. If the fermentation period is shorter than the above-mentioned general range, the fermentation does not proceed sufficiently and the effectiveness of the fermented product tends to decrease, while if the fermentation period is longer than 10 days, not only will no further increase in effectiveness be observed, but coloring and an increase in fermentation odor will occur, both of which are undesirable.

[0025] In carrying out the above fermentation treatment, in order to allow the microorganisms to utilize the plant components more effectively, an enzyme may be added to the suspension before or during inoculation of the microorganisms, or in some cases during the continuation of fermentation after inoculation, to subject the plant, which is the fermentation material, to an enzymatic hydrolysis treatment. In this case, as described above, at least one enzyme selected from protease, carbohydrate decomposition enzyme, pectic enzyme, and lipid decomposition enzyme can be used as the enzyme.

[0026] Regarding the treatment conditions such as pH, temperature, and time, if the enzyme treatment is carried out before fermentation, it is preferable to carry out the treatment for 1 to 24 hours at approximately the optimal pH and optimal temperature for the enzyme used, whereas if it is carried out in parallel with fermentation, the same conditions as those for the fermentation may be used.

[0027] After the above fermentation treatment is completed, the fermentation liquid is subjected to a heat sterilization treatment at 80 to 100°C for about 10 to 120 minutes to sterilize the microorganisms and, if an enzyme treatment is used in combination, to inactivate the enzymes. After the sterilization treatment, the fermentation liquid is used as is, or the liquid phase is separated by a solid-liquid separation means, generally and preferably, filtration or centrifugation, and if necessary, the pH is adjusted to pH 4 to 9, which is the pH range of ordinary cosmetics, and if necessary, the concentration is adjusted to an appropriate level by dilution or concentration, and then the liquid phase is used as a blending raw material for cosmetics. In some cases, the liquid phase after solid-liquid separation may be solidified by a conventional method such as spray drying or freeze drying, and further pulverized into a powder form if necessary.

[0028] Next, the extract of a plant of the genus Rosaceae will be described. The plant used as the extract material in the present invention may be any variety (including hybrids and subspecies). For example, Rosa Centifolia, Rosa Damascena, Rosa gallica, Rosa moschata, and Rosa alba can be mentioned.

[0029] The material used in the present invention may be any of the whole plant, leaves, inflorescences, stems, seeds, fruits, roots, placenta, etc. of the Rosa plant, but it is preferable to use the whole plant or inflorescences.

[0030] The preparation of the extract is carried out by first washing a plant of the genus Rosa (e.g., whole plant, inflorescence, etc.) with water in advance if necessary to remove foreign matter, then either as is or dried, and if necessary, shredded or crushed, and contacting it with an extraction solvent to carry out extraction. The extraction can be carried out by contacting it with an extraction solvent according to a conventional method such as the immersion method, but in addition to the immersion method, supercritical extraction methods can also be used.

[0031] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these can be used alone or in combination of two or more.

[0032] Among these extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention from the viewpoint of skin irritation and efficacy, and also because they can be widely applied to cosmetics.Preferred examples of hydrophilic solvents include the use of water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol) alone, or a mixed solvent of water and lower alcohols (particularly ethanol), or a mixed solvent of water and polyhydric alcohols (particularly 1,3-butylene glycol, glycerin), and among these, water alone or a mixed solvent of water and 1,3-butylene glycol is particularly preferred.

[0033] When a mixed solvent is used, the mixing ratio is preferably in the range of, for example, a volume ratio (hereinafter the same) of 1:10 to 20:1 for a mixed solvent of water and 1,3-butylene glycol, 1:10 to 25:1 for a mixed solvent of water and ethanol, and 1:10 to 20:1 for a mixed solvent of water and glycerin.

[0034] The weight ratio of the dried part of the rose (for example, the whole plant or inflorescence) to the extraction solvent is preferably in the range of 1:1 to 1:50.

[0035] In preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, an alkalinity regulator such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity regulator such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid may be blended with the extraction solvent to adjust the pH to a desired level.

[0036] The extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used, but for example, when water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent, the extraction temperature is preferably in the range of 0° C. to 80° C. The extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week).

[0037] Prior to or in parallel with the extraction treatment of the present invention, the extract of a plant of the genus Rosa may be subjected to a hydrolysis treatment as necessary, which may improve the storage stability of the extract of a plant of the genus Rosa and allow the extract to be used more effectively as an ingredient in cosmetic compositions.

[0038] When the extract is subjected to enzymatic hydrolysis, the enzymes used may be one or more selected from any one of the following enzyme groups: proteolytic enzymes such as actinase, papain, and pepsin; starch-degrading enzymes such as glucoamylase, α-amylase, and β-amylase; cellulose-degrading enzymes such as cellulase, hemicellulase, and pectinase; and lipolytic enzymes such as lipase. It is more preferable to use a combination of one or more enzymes selected from each of these enzyme groups.

[0039] The extract and fermentation product prepared as described above may be used as a cosmetic ingredient as it is after generally adjusting the pH to 3 to 8, or may be used at a desired concentration by concentrating under reduced pressure, etc. The extract may also be dried by a conventional method such as spray drying.

[0040] Uses of topical skin preparations containing the combination of the extract and fermented product of the present invention include, but are not limited to, topical skin preparations (topical medicinal components, topical quasi-drugs and cosmetics), such as milky lotions, creams, lotions, essences, gels, packs, sheet masks, slimming agents, etc.

[0041] The amount of the active ingredient according to the present invention in topical skin preparations (cosmetics and quasi-drugs) is generally in the range of 0.0002 to 1.0% by weight (solids weight %, the same applies below) in the case of basic cosmetics, and preferably in the range of 0.002 to 0.2% by weight, in terms of solid content.

[0042] Here, examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cacao oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, and other plant-derived oils and fats such as squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin wax. Examples of such fatty acids include hydrocarbons such as cocos and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0043] Examples of the surfactant include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of usable surfactants include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkyl benzyl ammonium salts, alkyl pyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethyl imidazolinium salts, N,N-dialkyl morpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethyl ammonio betaine, N,N,N-trialkyl-N-alkylene ammonio carboxybetaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropyl ammonio sulfobetaine, and the like.

[0044] Examples of the emulsifier or emulsification aid that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or its esters, lactose, soy-derived water-soluble polysaccharides, soy-derived protein and polysaccharide complexes, lanolin or its derivatives, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and its derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), and the like.

[0045] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acids octyldodecyl, seaweed extracts, estradiol, various amino acids and their derivatives.

[0046] Examples of thickening agents include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharides; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

[0047] Examples of anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or a derivative thereof.

[0048] Examples of preservatives and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and alkane bromide. These include kilisoquinolinium, resorcinol, jamal (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark distillate, fermented radish liquid, ethanol derived from plants such as sugar cane and corn, or 1,3-butylene glycol.

[0049] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0050] Anti-acne agents include, for example, sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0051] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybeans, adzuki beans, etc.), and the like.

[0052] Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0053] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.), and the like.

[0054] Examples of whitening agents include one or more selected from ellagic acid and its derivatives, resorcinol derivatives, potassium 4-methoxysalicylic acid, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate).

[0055] Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid. Examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. Examples of the active ingredient include one or more selected from kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, alpha-hydroxy acids, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acid derivatives, mulberry bark extract, chamomile extract, hydrolysate of rice bran extract, saxifrage extract, and white mustard extract or hydrolysates thereof.

[0056] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid ester salts such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate, and L-ascorbic acid ester salts such as sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate. Ascorbic acid sugar derivatives such as ascorbic acid 2-glucoside, L-ascorbic acid 5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, and caprylyl 2-glyceryl ascorbate; 6-acylated derivatives of these ascorbic acid sugar derivatives (the acyl group is a hexanoyl group, an octanoyl group, a decanoyl group, etc.); L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, and other L-ascorbic acid tetrafatty acid esters; -O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc., and hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), etc. Examples of the tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof) and amides of tranexamic acid (e.g., tranexamic acid methylamide). Examples of the resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of the 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid,Examples of nicotinic acid derivatives include nicotinamide (niacinamide) and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0057] The present invention will now be described in more detail with reference to Production Examples, Formulation Examples and Test Examples, but the present invention is not limited thereto. In the following, all parts are by weight and all % are by weight.

[0058] Production Example 1. Preparation of fermented Hibiscus plants (1) A suspension was made by adding 950g of purified water to 50g of dried flowers and calyx of a plant of the genus Hibiscus (Roselle), neutralizing the suspension with an alkali to a pH of about 6, and then sterilizing the suspension by heating at 80-90°C for 1 hour. 8 The cells were inoculated at 100 cells / mL and cultured stationarily for 3 days at 37° C. After the culture was completed, the culture solution was sterilized by heating and then filtered to obtain 737 g of a lactic acid bacteria fermentation solution (solid concentration: 3.32%).

[0059] Production Example 2. Preparation of fermented Hibiscus plants (2) The same procedure as in Production Example 1 was repeated except that the flowers and calyx of Rose of Sharon (Hibiscus syriacus) were used instead of roselle, to obtain 650 g of a lactic acid bacteria fermentation product solution (solid concentration: 2.54%).

[0060] Production Example 3. Preparation of fermented Hibiscus plants (3) The same procedure as in Production Example 1 was repeated except for using yeast Saccharomyces cerevisiae instead of lactic acid bacteria, to obtain 805 g of a yeast fermentation product solution (solid concentration: 3.18%).

[0061] Preparation Example 4. Preparation of Rosa plant extract solution (1) Petals of Rosa Damascena (Rosaceae) were dried, and 225g of purified water was added to 15g of the dried product, which was then soaked at 4℃, and 225g of 1,3-butylene glycol was added. Extraction was performed at 4℃, and the mixture was filtered to obtain 390g of a brown, transparent flower extract solution (solid concentration 1.07%).

[0062] Preparation Example 5. Preparation of Rosa genus plant extract solution (2) A brown, transparent flower extract solution (398 g) was obtained (solid concentration: 1.10%) by following the same process as in Preparation Example 1, except that the petals of Rosa Centifolia were used instead of the petals of Rosa Damascena used in Preparation Example 4.

[0063] Preparation Example 6. Preparation of Rosa genus plant extract solution (3) A brown, transparent flower extract solution (380 g) was obtained (solid content concentration: 1.03%) by following the same process as in Preparation Example 1, except that the petals of Rosa gallica were used instead of the petals of Rosa Damascena used in Preparation Example 4.

[0064] Example 1. Preparation of the composition A combination of an extract of a plant of the genus Rosa and a fermented product of a plant of the genus Hibiscus can be prepared, for example, by mixing the fermented product of any one of Production Examples 1 to 3 and the extract of any one of Production Examples 4 to 6. The mixing ratio is preferably 1:10 to 10:1, more preferably 1:3 to 3:1, in terms of solid content ratio, for the extract of a plant of the genus Rosa:the fermented product of a plant of the genus Hibiscus. For example, a composition shown in Table 1 can be prepared.

[0065] [Table 1] JPEG2025013031000001.jpg142137

[0066] Test Example 1. SASP gene expression evaluation test Human neonatal fibroblasts (NB1RGB) were cultured in Eagle's MEM medium containing 0.5% NCS at 9 × 10 4The cells were seeded at 100 cells / well and cultured at 37°C under 5% CO2 for one day. Next, the composition 7 shown in Table 1 was added to the same medium to a final concentration of 0.6% to prepare a sample solution according to the present invention, and a control solution was added to the same concentration of 40% 1,3-butylene glycol instead of the composition according to the present invention, and these were added to each well and cultured for another 24 hours. Then, ultraviolet light of about 100 mJ / cm2 was irradiated from the bottom of the culture vessel using a UV-B lamp (TL20W / 12RS, manufactured by Philips). After further culturing for 24 hours, the culture supernatant was collected and used in another test, while total RNA was extracted from the cells by the following method. The cells in each test group were collected with 0.5 mL of ISOGEN II reagent (manufactured by Nippon Gene Co., Ltd.). 200μL of RNase-free water was added to the collected cells, stirred and mixed, left at room temperature for 15 minutes, centrifuged at 15,000 rpm and 4℃ for 15 minutes in a centrifuge (TOMY / MX-160), and 500μL of the supernatant was collected. 500μL of isopropanol (Wako Pure Chemical Industries) was added to the collected supernatant, stirred and mixed, left at room temperature for 10 minutes, centrifuged at 15,000 rpm and 4℃ for 10 minutes to obtain a total RNA precipitate. 0.5mL of 75% ethanol was added to the total RNA, centrifuged at 15,000 rpm and 4℃ for 3 minutes to collect the precipitate. The supernatant was discarded, and this process was repeated twice. After completely removing the supernatant, the mixture was air-dried and dissolved in RNase-free water. The collected total RNA was subjected to reverse transcription reaction using a specified kit (PrimeScript RT reagent Kit with gDNA Eraser [Perfect Real Time] "Takara Bio") to synthesize cDNA. The synthesized cDNA was used as a sample to detect the expression of interleukin-1α (IL-1α) and matrix metalloproteinase-3 (MMP3), and the expression of the internal standard ACTB gene, using Thermal Cycler Dice (registered trademark) Real Time System Single (Takara Bio) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [Takara Bio].Here, ACTB (β-Actin) is one of the housekeeping genes (genes that are commonly expressed at a constant level in many tissues and cells, are constantly expressed, and are essential for cell maintenance and proliferation), and since its expression level is always constant, it is used as an internal standard in PCR experiments. The test results were obtained by comparing the expression levels of IL-1α and MMP-3 in each test group when the expression level of the ACTB gene was kept constant. The results were expressed as the relative values ​​of the expression levels of the genes in the other test groups when the expression level of each gene in the UV-unirradiated control group was set at 100. The expression rate of IL-1α and the expression rate of MMP-3 are shown in Table 2 and Table 3, respectively.

[0067] [Table 2] JPEG2025013031000002.jpg80152

[0068] As shown in Table 1, it was confirmed that fibroblasts (NB1RGB) irradiated with ultraviolet light (UV-B) significantly induced the expression of IL-1α. In contrast, it was confirmed that the composition of the present invention significantly suppressed the expression of IL-1α induced by irradiation with ultraviolet light (UV-B). As a result, the composition of the present invention can suppress the expression of IL-1α, an SASP factor, and inhibit the chain of cellular senescence.

[0069] [Table 3] JPEG2025013031000003.jpg80153

[0070] As shown in Table 3, it was confirmed that the expression of MMP-3 in fibroblasts (NB1RGB) was significantly induced by irradiation with ultraviolet light (UV-B). In contrast, it was confirmed that the composition of the present invention significantly suppressed the expression of MMP-3 induced by irradiation with ultraviolet light (UV-B). As a result, the composition of the present invention can suppress the activity of enzymes that degrade collagen, suppress the decrease in collagen in cells, and suppress the expression of MMP-3, an SASP factor, thereby suppressing the chain of cellular aging.

[0071] Test Example 2. Evaluation of cell senescence (SA-βGal activity) Human adult fibroblasts (NHDF) were cultured in Eagle's MEM medium containing 0.5% NCS at 9 × 10 4 The cells were seeded at 100 cells / well and cultured at 37°C for one day under 5% CO2. Next, the medium collected after 24 hours of culture from UV irradiation in the above Test Example 1 (SASP gene expression evaluation test) was added to each well and cultured for another 6 days. Thereafter, the medium was removed, SPiDER-βGal (SG02: Dojindo Chemical) was added, and the cells were incubated at 37°C for 30 minutes. Thereafter, the cells were detached with trypsin, and the fluorescence amount per cell (Ex=488 / Em538 nm) was measured using a flow cytometer. The results were expressed as relative values, with the value of the group to which the UV-unirradiated control culture supernatant was added being taken as 100. The results of Test Example 2 are shown in Table 4.

[0072] [Table 4] JPEG2025013031000004.jpg76153

[0073] As shown in Table 4, it was confirmed that SA-βGal activity, an indicator of aging, was significantly enhanced in fibroblasts (NB1RGB) upon irradiation with ultraviolet light (UV-B). In contrast, it was confirmed that the composition of the present invention suppresses SA-βGal activity, suggesting an effect of suppressing cellular aging.

[0074] Test Example 4. Collagenase activity evaluation test In Test Example 1 (SASP gene expression evaluation test), after 24 hours of incubation from UV irradiation, the collected culture supernatant was diluted 10-fold and used as a collagenase enzyme solution. Collagenase activity was measured using a collagenase assay kit from Primary Cell. 100 μL of the collagenase enzyme solution and 100 μL of FITC-labeled type I collagen substrate solution were added to a microtube. After 3 hours of reaction at 37°C, 600 μL of reaction stop solution was added, and unreacted collagen substrate was precipitated by centrifugation, and the fluorescence intensity of the supernatant (Ex=355, Em=460) was measured. The results are shown as relative values, with the value of the culture supernatant from the UV-unirradiated group taken as 100.

[0075] [Table 5] JPEG2025013031000005.jpg77170

[0076] As shown in Table 5, it was confirmed that collagenase activity was significantly enhanced in fibroblasts (NB1RGB) by irradiation with ultraviolet light (UV-B). In contrast, it was confirmed that the composition according to the present invention inhibits collagenase activity, thereby inhibiting the decrease in collagen and preventing and improving wrinkles and sagging of the skin or enlarged pores.

[0077] Formulation example 1. Lotion [Ingredients] Part Eucalyptus oil 0.2 Polyoxyethylene (5.5) Cetyl Alcohol 0.4 Composition 7 1.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Monoammonium glycyrrhizinate 0.5 Stearyl glycyrrhizinate 0.05 Isopropyl methylphenol 0.1 Aligned in 0.1 D-Pantothenyl alcohol 0.1 Salicylic acid 0.5 Urea 5.0 l-Menthol 0.9 dl-Menthol 0.2 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Methylparaben 0.1 Hinokitiol 0·003 Photosensor No. 201 0.002 Purified water (enough to make the total amount 100 parts)

[0078] Formulation example 2: Lotion [Ingredients] Part Glyceryl Caprylate 3.0 Polyglyceryl-10 Laurate 3.0 Cetanol 2.0 Behenyl Alcohol 2.0 Methylparaben 0.1 Composition 4 1.0 Ascorbic Acid 3.0 Glycyrrhizic acid 0.5 β-Glycyrrhetinic acid 0.05 Tocopherol nicotinate 0.1 Resorcinol 0.1 Zinc Oxide 2.0 dl-Camphor 0.5 Glycerin 2.0 1,3-Butylene glycol 5.0 Potassium hydroxide 0.5 Purified water (enough to make the total amount 100 parts)

[0079] Formulation example 3. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 5 was used instead of composition 4 contained in Formulation Example 2.

[0080] Formulation example 4: Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 6 was used instead of composition 4 contained in Formulation Example 2.

[0081] Formulation example 5. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 7 was used instead of composition 4 contained in Formulation Example 2.

[0082] Formulation example 6. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 8 was used instead of composition 4 contained in Formulation Example 2.

[0083] Formulation example 7. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 9 was used instead of composition 4 contained in Formulation Example 2.

[0084] Formulation example 7. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 10 was used instead of composition 4 contained in Formulation Example 2.

[0085] Formulation example 8. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 11 was used instead of composition 4 contained in Formulation Example 2.

[0086] Formulation example 9. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 12 was used instead of composition 4 contained in Formulation Example 2.

[0087] Formulation example 10. Lotion [Ingredients] Part Jojoba oil 1.0 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Methylparaben 0.1 Composition 7 1.0 Ascorbic Acid Glucoside 2.0 Tranexamic acid 2.0 ε-Aminocaproic acid 0.1 Sulfur 0.2 Estradiol 0.1 Glycerin 5.0 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Sodium metabisulfite 0.2 d-Camphor 0.1 Purified water (enough to make the total amount 100 parts)

[0088] Formulation example 11. Emulsion [Ingredients] Part Squalane 5.0 Cyclopentanesiloxane 1.0 Hexalan 3.0 Hexyldecyl Isostearate 1.0 Caprylic / Capric Triglyceride 1.0 Polyglyceryl-10 Laurate 5.0 Polyglyceryl-10 Isostearate 5.0 Ascorbyl Dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Composition 7 1.0 Ascorbic acid phosphate magnesium salt 3.0 Arbutin 3.0 Potassium hydroxide 0.5 Glycerin 3.0 1,3-Butylene glycol 2.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Tremella fuciformis polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Isopropyl methylphenol 0.1 Water-soluble collagen 1.0 Hydrolyzed Collagen 1.0 Sodium hyaluronate 1.0 Purified water (enough to make the total amount 100 parts)

[0089] Formulation example 12. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that composition 10 was used instead of composition 7 among the ingredients in Formulation Example 11.

[0090] Formulation example 13. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that composition 11 was used instead of composition 7 among the ingredients in Formulation Example 11.

[0091] Formulation example 14. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that composition 12 was used instead of composition 7 among the ingredients in Formulation Example 11.

[0092] Formulation example 15. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 2.0 parts of L-ascorbic acid-2-glucoside were used in place of 2.0 parts of magnesium ascorbyl phosphate.

[0093] Formulation example 16. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 2.0 parts of tranexamic acid were used in place of 2.0 parts of magnesium ascorbyl phosphate and 0.5 parts of potassium hydroxide.

[0094] Formulation example 17. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 3.0 parts of nicotinamide was used in place of 2.0 parts of magnesium ascorbyl phosphate in Formulation Example 11.

[0095] Prescription example 18. Cream [Ingredients] Part Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Hexyldecyl Isostearate 5.0 Lauroyl glutamate di(octyldodecyl / phytosteryl / behenyl) 5.0 Glyceryl Caprylate 1.0 Glyceryl stearate 1.0 Isostearyl glyceryl 3.0 γ-Oryzanol 0.1 Behenyl Alcohol 2.0 Palmitic acid 2.5 D-Pantothenyl Alcohol 3.0 Allantoin 0.1 Riboflavin 0.01 Resorcinol 0.1 Benzalkonium chloride 0.05 Urea 3.0 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Ammonium glycyrrhizinate 0.1 Composition 7 2.0 Lactic acid fermented rice 2.0 Hydrogenated lecithin 0.5 Hydrogenated lysolecithin 0.5 Oil-soluble ginseng extract 2.0 Xanthan gum 1.0 Zinc oxide 0.5 dl-Camphor 0.3 l-Menthol 0.5 Purified water (enough to make the total amount 100 parts)

[0096] Example 19. Pack [Ingredients] Part Dipropylene glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetanol 3.0 Behenyl Alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Ammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.5 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Composition 10 2.0 Xanthan gum 2.0 Polyglyceryl-6 Myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated Lecithin 3.0 Hydroxylated Lecithin 3.0 Purified water (enough to make the total amount 100 parts)

[0097] Formulation example 20. Sheet mask A sheet mask is obtained by impregnating a nonwoven fabric with the following components. [Ingredients] Part Composition 7 2.0 Glycerin 3.0 1,3-Butylene glycol 2.0 L-Ascorbic Acid 2-Glucoside 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 1.0 Water-soluble collagen 1.0 Sodium hyaluronate 1.0 Eelgrass extract 1.0 Rice extract hydrolyzate 1.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0098] Prescription example 21. Beauty serum [Ingredients] Part Ethanol 2.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Methylparaben 0.1 Composition 1 2.0 Citric acid 0.3 Sodium citrate 0.6 Purified water (enough to make the total amount 100 parts)

[0099] Prescription example 22. Beauty serum A beauty serum was obtained in the same manner as in Formulation Example 21, except that composition 2 was used instead of composition 1 in the beauty serum of Formulation Example 21.

[0100] Prescription example 23. Beauty serum A beauty serum was obtained in the same manner as in Formulation Example 21, except that composition 3 was used instead of composition 1 in the beauty serum of Formulation Example 21.

[0101] Prescription example 24. Beauty serum A beauty serum was obtained in the same manner as in Formulation Example 21, except that composition 13 was used instead of composition 1 in the beauty serum of Formulation Example 21.

[0102] Prescription example 25. Beauty serum A beauty serum was obtained in the same manner as in Formulation Example 21, except that composition 14 was used instead of composition 1 in the beauty serum of Formulation Example 21.

[0103] Prescription example 26. Beauty serum A beauty serum was obtained in the same manner as in Formulation Example 21, except that composition 15 was used instead of composition 1 in the beauty serum of Formulation Example 21.

Claims

[Claim 1] An external skin preparation for preventing cellular aging, which contains a fermented product of a plant belonging to the Hibiscus genus of the Malvaceae family and an extract of a plant of the Rosaceae family.

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