Production certificate
Next-generation sequencing and international database registration of Nishikigoi DNA data in production certificates address the challenge of reliable genetic verification, improving identification and traceability.
Patent Information
- Application Number
- JP2023130484
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- Filing Date
- 2023-07-24
- Publication Date
- 2025-02-05
AI Technical Summary
Current methods for identifying and verifying Nishikigoi fish lack a reliable and standardized approach, particularly in terms of genetic verification.
Utilizing next-generation sequencing to extract DNA from Nishikigoi and registering the sequence data in international databases like DDBJ/NCBI/ENA, with unique identifiers included in production certificates.
Enhances the ease and reliability of Nishikigoi identification through genetic verification, providing a standardized and traceable method.
Abstract
Description
[Technical field]
[0001] The present invention relates to an information-carrying card that certifies the breeder of a particular Nishikigoi. [Background technology]
[0002] Traditionally, Nishikigoi breeders have issued production certificates for the Nishikigoi they produce. Production certificates usually include a photograph of the individual to be certified, as well as the breed name, production date, size, issue date, and breeder name. Summary of the Invention [Problem to be solved by the invention]
[0003] There has been a demand for a way to identify Nishikigoi other than by photograph, breed name, and size. [Means for solving the problem]
[0004] Nishikigoi genomes are sequenced and registered in international DNA databases such as DDBJ, NCBI, and ENA. The resulting registration identifier can then be included on the production certificate, allowing the Nishikigoi to be identified.
[0005] A preferred embodiment of the present invention comprises: DNA is extracted from the gills of Nishikigoi carp, and genome-derived sequence data is obtained using a workflow that uses a general next-generation sequencer. The registration identifier obtained by registering the aforementioned sequence data in international DNA databases such as DDBJ, NCBI, and ENA is then included in the production certificate. Effect of the Invention
[0006] The certificate of this invention makes it easier to identify Nishikigoi. EXAMPLES
[0007] DNA was extracted from the gills of two Nishikigoi, Ginrin Showa and Ogon Ryu, and genome-derived sequence data was obtained using a next-generation sequencer. They were registered in the DDBJ SRA database and given the identifiers DRS315084 and DRS315082, respectively. DRS315084 and DRS315082 were listed on the respective production certificates. Ginrin Showa can be found in the international DNA database NCBI at the URL below (https: / / www.ncbi.nlm.nih.gov / biosample / 36345085). Ogon Ryu can be found in the international DNA database NCBI at the URL below (https: / / www.ncbi.nlm.nih.gov / biosample / 36345083). [Industrial Applicability]
[0008] The present invention can be used in the fields of agriculture, forestry and fisheries, where Nishikigoi are produced and sold.
Claims
1. A certificate for Nishikigoi, A certificate listing the specific Koi breeder and an identifier in the international database of sequence data derived from said Koi genome.
2. 2. The certificate of claim 1, The sequence data derived from the Nishikigoi genome is derived from the gills of the Nishikigoi mentioned above.