External plate movable stage system for automated cell microscopic imaging and analysis

Abstract

To provide an external plate movable stage system for automated cell microscopic imaging and analysis.SOLUTION: A system comprises an automatic stage module, a control module and an image positioning module. The automatic stage module comprises a stage adjustment structure and a movable stage. The stage adjustment structure is detachably positioned on one side of a microscope. The movable stage is located between the objective lens of the microscope and a sample stage and is driven by the stage adjustment structure to move in X-axis and Y-axis directions. The control module is configured to control the movable stage to move in a scanning manner so that all parts of a sample plate sequentially pass through the objective lens of the microscope to capture a plurality of sub-images. The image positioning module is configured to automatically adjust the focal length of the microscope lens of the microscope, and upon detecting the displacement of the sub-images, automatically adjust the sub-images to a correct position, and synthesize the sub-images by an image processing method to generate a full image.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to a cell microscopy imaging and analysis system, and particularly to an externally attached petri dish moving type automatic cell microscopy imaging and analysis system.

Background Art

[0002] During the cell culture period, it is necessary to frequently take out the cell culture petri dish from the cell culture device in an artificial manner at regular intervals, observe the growth state of the cells with a microscope, determine the cell harvesting time, and evaluate the cell culture quality of the lot. In the cell culture process, the cell culture quality is mainly evaluated by observing cell morphology, measuring cell viability, analyzing cell proliferation, detecting cell contamination, etc. Among them, the observation of cell morphology by a microscope is currently one of the most common, direct, and rapid methods for evaluating cell culture quality. By observing the shape and size of the cells, the degree of cell adhesion to the bottom of the cell culture petri dish, and the growth density (confluence) of the cells in the petri dish, the cell culture quality is evaluated and the cell harvesting time is predicted.

[0003] In conventional general confluence evaluation, mainly, the operator of the experiment places the cell culture petri dish on the microscope stage and artificially observes the cell distribution status and growth density in the cell culture petri dish below the microscope with the naked eye through the eyepiece. Also, by combining the movement of the cell culture petri dish on the stage performed by the operator's hand and the movement of the cell culture petri dish in the XYZ axes of the microscope device adjusted using the microscope knob, the growth state of the cells in the cell culture petri dish is completely observed, and confluence analysis is performed based on the operator's work experience. However, when observing the confluence of cells with a microscope during the cell culture period, the following drawbacks may occur.

[0004] 1. During the observation process, the risk of cell contamination in the cell culture petri dish may increase due to the operator's hand moving the cell culture petri dish or contacting the cell culture petri dish multiple times.

[0005] 2. Due to the long artificial observation time, temperature changes may occur in the cells in the cell culture dish due to human body temperature or environmental temperature.

[0006] 3. By manually moving the dish for observation without positioning, dead spots for observation or photography may occur, resulting in the inability to comprehensively observe and analyze the cells.

[0007] In order to solve the problem that cell contamination may occur by moving the dish with the operator's hand, there are currently microscope observation systems with an automatic dish moving function on the market. Most of the automatic dish moving functions are directly attached and installed to the components of the microscope to form a microscope observation system with an automatic dish moving function. However, usually, this type of microscope observation system has the following drawbacks.

[0008] 1. It cannot be directly attached and installed alone to a general commercially available microscope device, so it is necessary to redesign for each microscope device, increasing the manufacturing cost.

[0009] 2. Since the automatic dish moving function is directly attached and installed to the components of the microscope, the original design of the microscope device is changed, resulting in the inability to coexist manual dish moving and automatic dish moving.

[0010] 3. The motor of the automatic dish moving device may generate vibrations in the microscope device during the operation process. As a result, it is easy to occur misalignment of the image, focus error, or displacement of the cell culture dish.

[0011] The problems mentioned above may affect the operator when observing cells and performing confluence analysis. In addition, the current microscope observation system equipped with such an automatic petri dish moving function only has the function of moving the cell culture petri dish, and does not have the function of recognizing and analyzing the confluence and growth density of cells. Therefore, it is still necessary to observe manually with a microscope by the naked eye and perform confluence analysis of cells based on the operator's work experience. Therefore, in order to solve the above-mentioned conventional problems, it is necessary to research and develop a new automated petri dish moving and cell microscope photographing / analyzing system.

Summary of the Invention

Problems to be Solved by the Invention

[0012] In view of the above, in order to solve the above-mentioned conventional problems, the present invention provides an external petri dish moving type automatic cell microscope photographing / analyzing system.

Means for Solving the Problems

[0013] The present invention provides an external petri dish moving type automatic cell microscope photographing and analyzing system for assisting the observation of cell samples by a microscope and avoiding the vibration of the microscope. The external petri dish moving type automatic cell microscope photographing and analyzing system includes an automatic stage module, a control module, and an image positioning module. The automatic stage module includes a stage adjustment structure and a moving stage connected to the stage adjustment structure. The stage adjustment structure is removably provided on one side of the microscope. When the stage adjustment structure is provided on one side of the microscope, the moving stage is located between the objective lens of the microscope and the mounting table, and moves in the X-axis and Y-axis directions under the drive of the stage adjustment structure. The control module is connected to the stage adjustment structure and controls the stage adjustment structure to move the moving stage. Further, the control module is used to sequentially pass all parts of the sample petri dish through the objective lens of the microscope by controlling the moving stage to move in a scanning manner, so as to obtain a plurality of sub-images. The image positioning module is connected to the control module. The image positioning module is used to automatically adjust the focal length of the lens of the microscope. When detecting the displacement of the sub-image, it automatically adjusts the sub-image to the correct position and synthesizes the sub-images by an image processing method to generate an overall image.

[0014] The external petri dish moving type automatic cell microscope photographing and analyzing system further includes an image recognition module connected to the control module and the microscope. The image recognition module is used to obtain the growth density and coverage rate of the cell sample in the sample petri dish by receiving and analyzing the overall image.

[0015] The moving stage further includes an XY-axis stage. The XY-axis stage is used to horizontally move the sample petri dish in the X-axis and Y-axis directions between the objective lens of the microscope and the mounting table under the drive of the stage adjustment structure.

[0016] The externally attached petri dish movable automatic cell microscope imaging and analysis system further includes a stage adjustment structure of the automatic stage module and an accommodation space for accommodating the movable stage.

[0017] The externally attached petri dish movable automatic cell microscope imaging and analysis system further includes a hole and a sealing shutter corresponding to the hole. The hole is used for taking in and out the stage adjustment structure and the movable stage of the automatic stage module. The sealing shutter is used to prevent the contamination of the stage adjustment structure and the movable stage by foreign objects in the working environment.

[0018] The movable stage further includes a removable connection rod.

[0019] The stage adjustment structure further includes a removable mounting table.

[0020] The externally attached petri dish movable automatic cell microscope imaging and analysis system further includes a conveyor belt.

[0021] Another aspect of the present invention provides another externally attached petri dish moving type automatic cell microscope photographing and analyzing system including a microscope and an externally attached petri dish moving device. The microscope is used for observing cell samples. The externally attached petri dish moving device is externally connected to the microscope to assist in observing the cell samples by the microscope and is used to avoid the vibration of the microscope. The externally attached petri dish moving device includes an automatic stage module, a control module, and an image positioning module. The automatic stage module includes a stage adjustment structure and a moving stage connected to the stage adjustment structure. The stage adjustment structure is detachably provided on one side of the microscope. The moving stage is located between the objective lens of the microscope and the stage when the stage adjustment structure is provided on one side of the microscope, and moves in the X-axis and Y-axis directions under the drive of the stage adjustment structure. The control module is connected to the stage adjustment structure and controls the stage adjustment structure to move the moving stage. Further, the control module is used to obtain a plurality of sub-images by moving the moving stage in a scanning manner so that all parts of the sample petri dish pass through the objective lens of the microscope in sequence. The image positioning module is connected to the control module. The image positioning module is used to automatically adjust the focal length of the lens of the microscope. When detecting the displacement of the sub-image, it automatically adjusts the sub-image to the correct position and synthesizes the sub-images by an image processing method to generate an overall image.

[0022] The externally attached petri dish moving type automatic cell microscope photographing and analyzing system further includes an image recognition module connected to the control module and the microscope. The image recognition module is used to obtain the growth density and coverage rate of the cell sample in the sample petri dish by receiving and analyzing the overall image.

[0023] The moving stage of the externally attached petri dish moving device further includes an XY-axis stage. The XY-axis stage is used to horizontally move the sample petri dish in the X-axis and Y-axis directions between the objective lens of the microscope and the stage under the drive of the stage adjustment structure.

[0024] The externally attached petri dish moving device further includes a stage adjustment structure of the automatic stage module and an accommodation space for accommodating the moving stage.

[0025] The externally attached petri dish moving device further includes a hole and a sealing shutter corresponding to the hole. The hole is used for the stage adjustment structure of the automatic stage module and the entry and exit of the moving stage. The sealing shutter is used to prevent contamination of the stage adjustment structure and the moving stage by foreign matter in the working environment.

[0026] The moving stage of the externally attached petri dish moving device further includes a removable connecting rod.

[0027] The stage adjustment structure of the externally attached petri dish moving device further includes a removable mounting table.

[0028] The externally attached petri dish moving device further includes a conveyor belt.

Advantages of the Invention

[0029] Generally speaking, the present invention provides an externally attached petri dish moving type automatic cell microscopy imaging and analysis system. By the automatic externally attached petri dish moving device of the present invention, the number of times of artificially contacting the cell culture petri dish can be reduced, so the risk of cell contamination is reduced. In addition, by automatically moving and observing the petri dish by the automatic externally attached petri dish moving device of the present invention, the risk of temperature change in the cells caused by human body temperature or environmental temperature can be reduced. Moreover, since the externally attached petri dish moving device of the present invention is externally connectable, it can be separately attached and installed to a general commercially available microscope device, enabling manual and automatic petri dish movement and reducing the occurrence of vibration during the observation of cell culture. In addition, by analyzing the image of the sample petri dish by the image recognition of the present invention, relatively accurate growth density and coverage rate of the cell sample can be obtained, and experimental errors caused by the subjective judgment of various operators are reduced.

Brief Description of the Drawings

[0030]

Figure 1

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Figure 6

Embodiments for Carrying out the Invention

[0031] Subsequently, in order to make the advantages, spirit and features of the present invention easier and clearer to understand, specific embodiments will be used and described in detail with reference to the drawings. It should be noted that these specific embodiments are only representative specific embodiments of the present invention, and the specific methods, devices, conditions, materials, etc. exemplified do not limit the present invention or the corresponding specific embodiments. Moreover, each component in the drawings is used only to represent their relative positions and is not described based on actual ratios. Also, the step numbers of the present invention are only for distinguishing different steps and do not represent the order of the steps. The above is explained in advance.

[0032] Refer to FIGS. 1 and 2 together. FIG. 1 shows a functional block diagram of an external petri dish moving type automatic cell microscope imaging and analysis system 1 based on a specific embodiment of the present invention. FIG. 2 shows a schematic structural diagram of the external petri dish moving type automatic cell microscope imaging and analysis system 1 based on a specific embodiment of the present invention. The external petri dish moving type automatic cell microscope imaging and analysis system 1 in this specific embodiment is used to assist in observing cell samples by the microscope 2 and to avoid the vibration of the microscope 2. As shown in FIG. 1, the external petri dish moving type automatic cell microscope imaging and analysis system 1 in this specific embodiment includes an automatic stage module 11, a control module 12, and an image positioning module 13. The automatic stage module 11 includes a stage adjustment structure 111 and a moving stage 112 connected to the stage adjustment structure. The stage adjustment structure 111 is detachably provided on one side of the microscope 2. And when the stage adjustment structure is provided on one side of the microscope, the moving stage 112 is located between the objective lens 201 of the microscope 2 and the mounting table 202, and moves in the X-axis and Y-axis directions under the drive of the stage adjustment structure 111. The control module 12 is connected to the stage adjustment structure 111 and controls the stage adjustment structure 111 to move the moving stage 112. Also, the control module 12 is used to move the moving stage 112 in a scanning manner to pass all parts of the sample petri dish through the objective lens 201 of the microscope 2 in sequence to obtain a plurality of sub-images. In addition, a plurality of sub-images may be obtained by controlling the scanning method of the moving stage 112 in a manner of repeating spot scanning, line scanning, area scanning, etc. In practice, the scanning method is not limited to this and may be adjusted and designed according to the needs of the user. Also, the image positioning module 13 is connected to the control module 12. The image positioning module 13 is used to automatically adjust the focal length of the lens of the microscope 2. When detecting the displacement of the sub-image, it automatically adjusts the sub-image to the correct position and synthesizes the sub-images by an image processing method to generate an overall image.In the present specific embodiment, the external petri dish moving type automatic cell microscope photographing and analyzing system 1 can move in the X-axis and Y-axis directions by the stage adjustment structure 111. For the movement in the Z-axis direction, it can be realized by directly controlling the focal length of the lens by the image positioning module 13. Since the external petri dish moving type automatic cell microscope photographing and analyzing system 1 in the present specific embodiment is detachably installed on the microscope device, it is not necessary to change the main body design of the microscope device and the design of the handle of the microscope device. Thereby, the generation of vibration is reduced and the manufacturing cost is lowered. In addition, the external petri dish moving type automatic cell microscope photographing and analyzing system in the present specific embodiment can reduce the number of times of artificially contacting the cell culture petri dish, so the risk of cell contamination is reduced.

[0033] Refer to FIG. 3. FIG. 3 shows a functional block diagram of an external petri dish moving type automatic cell microscope photographing and analyzing system 1' based on another specific embodiment of the present invention. As shown in FIG. 3, the present specific embodiment is different from the above-described specific embodiment in the following points. That is, the external petri dish moving type automatic cell microscope photographing and analyzing system 1' in the present specific embodiment further includes a control module 12 and an image recognition module 14 connected to the microscope 2. The image recognition module 14 is used to obtain the growth density and coverage rate of the cell sample in the sample petri dish by receiving and analyzing the whole image. In addition, the image recognition module 14 of the present invention may receive and analyze the image of the sample petri dish by an artificial intelligence engine to obtain a relatively accurate growth density and coverage rate of the cell sample. Thereby, the experimental error caused by the subjective judgment of various operators is reduced. It should be noted that, as other modules and functions of the external petri dish moving type automatic cell microscope photographing and analyzing system 1' in the present specific embodiment are substantially the same as the corresponding modules in the above-described specific embodiment, they will not be described in detail again here.

[0034] Refer to FIG. 4. FIG. 4 shows a functional block diagram of an external petri dish moving type automatic cell microscope photographing and analyzing system 1'' based on another specific embodiment of the present invention. This specific embodiment is different from the above-described specific embodiment in the following points. That is, the moving stage 112 of the external petri dish moving type automatic cell microscope photographing and analyzing system 1'' in this specific embodiment further includes an XY-axis stage 1121. The XY-axis stage 1121 is used to horizontally move the sample petri dish in the X-axis and Y-axis directions between the objective lens 201 (shown in FIG. 2) of the microscope 2 and the stage 202 (shown in FIG. 2) under the drive of the stage adjustment structure 111. Also, in another specific embodiment, the external petri dish moving type automatic cell microscope photographing and analyzing system further includes a conveyor belt (not shown), and the conveyor belt is used to move the stage 202 of the microscope 2 horizontally and vertically. It should be noted that since the other modules and functions of the external petri dish moving type automatic cell microscope photographing and analyzing system 1'' in this specific embodiment are substantially the same as the corresponding modules in the above-described specific embodiment, they will not be described in detail again here.

[0035] Refer to FIGS. 1 and 5. FIG. 5 shows a schematic structural diagram of an external petri dish moving type automatic cell microscope photographing and analyzing system 1''' based on another specific embodiment of the present invention. As shown in FIG. 5, the external petri dish moving type automatic cell microscope photographing and analyzing system 1''' in this specific embodiment further includes an accommodation space 21 for accommodating the stage adjustment structure 111 and the moving stage 112 of the automatic stage module 11. In addition, the external petri dish moving type automatic cell microscope photographing and analyzing system 1''' in this specific embodiment may further include a hole 31 and a sealing shutter 32 corresponding to the hole. The hole 31 is used for the entry and exit of the stage adjustment structure 111 and the moving stage 112 of the automatic stage module 11. The sealing shutter 32 is used to prevent the contamination of the stage adjustment structure 111 and the moving stage 112 by foreign matters in the working environment. When the control module 12 of the external petri dish moving type automatic cell microscope photographing and analyzing system 1''' in this specific embodiment controls the stage adjustment structure 111 to operate the moving stage 112, the sealing shutter 32 is opened and the moving stage 112 is extended. On the other hand, when the control module 12 controls the stage adjustment structure 111 to stop the operation of the moving stage 112, the moving stage 112 passes through the hole 31 and returns to the accommodation space 21. At this time, the sealing shutter 32 closes. In addition, in another specific embodiment, the moving stage may further include a removable connection rod 41. In addition, in another specific embodiment, the stage adjustment structure in the external petri dish moving type automatic cell microscope photographing and analyzing system may further include a removable mounting table (not shown). It should be noted that, as a point to be noted, since the other modules and functions of the external petri dish moving type automatic cell microscope photographing and analyzing system 1''' in this specific embodiment are substantially the same as the corresponding modules in the above-described specific embodiment, they will not be described in detail again here.

[0036] In another aspect of the present invention, there is provided an externally attached petri dish moving type automatic cell microscope photographing and analyzing system that removably connects an externally attached petri dish moving device and a microscope. Here, refer to FIG. 6. FIG. 6 shows a functional block diagram of an externally attached petri dish moving type automatic cell microscope photographing and analyzing system 3 based on a specific embodiment of the present invention. As shown in FIG. 6, the externally attached petri dish moving type automatic cell microscope photographing and analyzing system 3 in this specific embodiment includes a microscope 10 and an externally attached petri dish moving device 30. The microscope 10 is used for observing a cell sample. The externally attached petri dish moving device 30 is externally connected to the microscope 10 to assist in observing the cell sample by the microscope 10 and is used to avoid the vibration of the microscope 10. The externally attached petri dish moving device 30 includes an automatic stage module 11, a control module 12, and an image positioning module 13. The automatic stage module 11 includes a stage adjustment structure 111 and a moving stage 112 connected to the stage adjustment structure. The stage adjustment structure 111 is removably provided on one side of the microscope 10. When the stage adjustment structure is provided on one side of the microscope, the moving stage 112 is located between the objective lens (not shown) of the microscope 10 and the stage (not shown), and moves in the X-axis and Y-axis directions under the drive of the stage adjustment structure 111. The control module 12 is connected to the stage adjustment structure 111 and controls the stage adjustment structure 111 to move the moving stage 112. Further, the control module 12 is used to obtain a plurality of sub-images by passing all parts of the sample petri dish through the objective lens (not shown) of the microscope 10 in order by controlling the moving stage 112 to move in a scanning manner. The image positioning module 13 is connected to the control module 12. The image positioning module 13 is used to automatically adjust the focal length of the lens of the microscope 10. When detecting the displacement of the sub-image, it automatically adjusts the sub-image to the correct position and synthesizes the sub-images by an image processing method to generate an overall image. It should be noted that other modules and functions of the externally attached petri dish moving type automatic cell microscope photographing and analyzing system 3 in this specific embodiment are substantially the same as the corresponding modules in the above-described specific embodiment, and thus will not be described in detail again here.

[0037] In summary, the present invention provides an externally attached petri dish moving type automatic cell microscope photographing and analyzing system. By the automatic externally attached petri dish moving device of the present invention, the number of times of artificially contacting the cell culture petri dish can be reduced, so the risk of cell contamination is reduced. Further, by automatically moving and observing the petri dish by the automatic externally attached petri dish moving device of the present invention, the risk that the temperature of the cells changes due to the body temperature of a person or the environmental temperature can be reduced. Moreover, since the externally attached petri dish moving device of the present invention is externally connectable, it can be individually attached and installed to a general commercially available microscope device, enabling manual and automatic petri dish movement and reducing the occurrence of vibration during the observation of cell culture. In addition, by analyzing the image of the sample petri dish by the image recognition of the present invention, a relatively accurate growth density and coverage rate of the cell sample can be obtained, and experimental errors caused by the subjective judgment of various operators are reduced.

[0038] The detailed description of the above preferred specific embodiments is intended to more clearly describe the features and spirit of the present invention, and is not intended to limit the scope of the present invention by the preferred specific embodiments disclosed above. Rather, the above description is intended to cover configurations having various modifications and equivalences within the scope of the claims sought to be patented by the present invention. Therefore, the claims sought to be patented by the present invention should be construed as broadly as possible based on the above description so as to cover all possible modifications and configurations having equivalences.

Explanation of Signs

[0039] 1, 1’, 1’’, 1’’’, 3 Externally attached petri dish moving type automatic cell microscope photographing and analyzing system 11 Automatic stage module 111 Stage adjustment structure 112 Moving stage 1121 XY-axis stage 12 Control module 13 Image positioning module 14 Image recognition module 2,10 microscope 201 objective lens 202 stage 21 accommodation space 30 externally attached petri dish moving device 31 hole 32 sealing shutter 41 connecting rod

Claims

1. An automatic cell microscope photographing and analysis system with an external petri dish moving mechanism for assisting in observation of a cell sample by a microscope and avoiding vibration of the microscope, comprising: an automatic stage module including a stage adjustment structure and a moving stage connected to the stage adjustment structure, the stage adjustment structure being detachably provided on one side of the microscope, the moving stage being positioned between an objective lens and a mounting table of the microscope when the stage adjustment structure is provided on one side of the microscope, and being driven by the stage adjustment structure to move in the X-axis and Y-axis directions; a control module connected to the stage adjustment structure and configured to control the stage adjustment structure to move the moving stage, the control module being configured to control the moving stage to move in a scanning manner, so that all portions of a sample dish are passed through the objective lens of the microscope in sequence to obtain a plurality of sub-images; An external petri dish moving automatic cell microscope photography and analysis system that includes an image positioning module connected to the control module, the image positioning module being used to automatically adjust the focal length of the microscope lens, and which, upon detecting a displacement of the sub-image, automatically adjusts the sub-image to the correct position and synthesizes the sub-images using an image processing method to generate an overall image.

2. The external dish-moving automatic cell microscope photography and analysis system of claim 1 further includes an image recognition module connected to the control module and the microscope, the image recognition module receiving and analyzing the overall image to obtain the growth density and coverage rate of the cell sample in the sample dish.

3. 2. The external petri dish moving automatic cell microscope photography and analysis system of claim 1, wherein the moving stage further includes an XY-axis stage, and the XY-axis stage is driven by the stage adjustment structure to horizontally move the sample petri dish in the X-axis and Y-axis directions between the objective lens of the microscope and the mounting table.

4. The external Petri dish moving type automatic cell microscope photographing and analysis system according to claim 1 , further comprising an accommodation space for accommodating the stage adjustment structure and the moving stage of the automatic stage module.

5. 2. The external Petri dish moving automatic cell microscope photography and analysis system of claim 1, further comprising a hole and a sealing shield corresponding to the hole, the hole being used for inserting and removing the stage adjustment structure and the moving stage of the automatic stage module, and the sealing shield being used for preventing contamination of the stage adjustment structure and the moving stage by foreign matter in the working environment.

6. The external Petri dish moving automatic cell microscope imaging and analysis system according to claim 1 , wherein the moving stage further includes a removable connecting rod.

7. The external petri dish moving type automatic cell microscope photographing and analysis system according to claim 1 , wherein the stage adjustment structure further includes a removable mounting base.

8. The external petri dish moving automatic cell microscope photographing and analysis system according to claim 1 , further comprising a conveyor belt.

9. a microscope for observing cell samples; an external petri dish moving device that is connected to the microscope from the outside and is used to assist the observation of the cell sample by the microscope and to avoid vibration of the microscope; The external petri dish moving device is an automatic stage module including a stage adjustment structure and a moving stage connected to the stage adjustment structure, the stage adjustment structure being detachably provided on one side of the microscope, the moving stage being positioned between an objective lens and a mounting table of the microscope when the stage adjustment structure is provided on one side of the microscope, and being driven by the stage adjustment structure to move in the X-axis and Y-axis directions; a control module connected to the stage adjustment structure and configured to control the stage adjustment structure to move the moving stage, the control module being configured to control the moving stage to move in a scanning manner, so that all portions of a sample dish are passed through the objective lens of the microscope in sequence to obtain a plurality of sub-images; An external petri dish moving automatic cell microscope photography and analysis system that includes an image positioning module connected to the control module, the image positioning module being used to automatically adjust the focal length of the microscope lens, and which, upon detecting a displacement of the sub-image, automatically adjusts the sub-image to the correct position and synthesizes the sub-images using an image processing method to generate an overall image.

10. The external dish-moving automatic cell microscope photography and analysis system of claim 9 further includes an image recognition module connected to the control module and the microscope, the image recognition module receiving and analyzing the overall image to obtain the growth density and coverage rate of the cell sample in the sample dish.

11. The external petri dish moving automatic cell microscope photography and analysis system of claim 9, wherein the moving stage of the external petri dish moving device further includes an XY-axis stage, and the XY-axis stage is driven by the stage adjustment structure to horizontally move the sample petri dish in the X-axis and Y-axis directions between the objective lens of the microscope and the mounting table.

12. The external petri dish moving automatic cell microscope photography and analysis system of claim 9, wherein the external petri dish moving device further includes an accommodation space for accommodating the stage adjustment structure and the moving stage of the automatic stage module.

13. The external Petri dish moving device further includes a hole and a sealing shield corresponding to the hole, the hole is used for inserting and removing the stage adjustment structure and the moving stage of the automatic stage module, and the sealing shield is used to prevent contamination of the stage adjustment structure and the moving stage by foreign matter in the working environment.

14. The external Petri dish moving automatic cell microscope photographing and analysis system according to claim 9 , wherein the moving stage of the external Petri dish moving device further includes a removable connecting rod.

15. 10. The external petri dish moving automatic cell microscope photographing and analysis system according to claim 9, wherein the stage adjustment structure of the external petri dish moving device further includes a removable mounting table.

16. The external petri dish moving automatic cell microscope photographing and analysis system according to claim 9 , wherein the external petri dish moving device further includes a conveyor belt.

Images