Pest control method, pest control agent, and method for producing the same

Abstract

To provide selective control of specific pests by utilizing insect secretions with minimal impact on mammals including humans and birds.SOLUTION: A control agent containing, as a control component, one or more hydrocarbons having 23 to 29 carbon atoms is applied to a control target site for one or more pests selected from the group consisting of silvanid beetles, flat bark beetles, anobiid beetles, darkling beetles, minute brown scavenger beetles, booklice, tineid moths, wasps and bees, pill bugs, sow bugs, and velvet mites, thereby activating a specific region of the antennal lobe of the pest and inducing an aversive behavior to drive the pest away from the target site.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present disclosure relates to a technique for controlling pests using components contained in insect secretions. [Background technology]

[0002] It is known that pests can occur in food factories due to mold that grows on food, flour, grains, etc. Pharmaceutical factories require a higher level of hygiene than general manufacturing factories. In these factories, pest control measures are necessary to prevent product contamination due to pest contamination. One method of pest control is to use pest control agents to prevent pests from entering the target space.

[0003] Conventionally, pest control agents used in food factories and pharmaceutical factories contain pest control and insecticidal components that are highly safe for humans, such as pyrethroid compounds. For example, Patent Document 1 proposes a sealant containing a pyrethroid compound as an insecticide and / or pest repellent, with the aim of preventing outdoor ants, cockroaches, and the like from entering indoors, and preventing massive infestations of booklice from entering indoors through cracks in concrete or gypsum boards, gaps between walls and baseboards, and the like.

[0004] Pyrethroid compounds are generally effective against a wide variety of pests, and have the advantages of being fast-acting, long-lasting, and effective at low doses. However, pyrethroid compounds have the drawback of easily developing resistance in pests. In recent years, therefore, progress has been made in the practical application of pesticides and insecticides that utilize the mechanisms of insect pheromones, which are effective against pests resistant to existing insecticides.

[0005] Known insect control agents that utilize insect pheromones include those that utilize insect sex pheromones for mass-killing or mating disruption. For example, Patent Document 2 discloses a solid insecticidal composition containing an insecticidal active ingredient for mass-killing Dipteran insects such as house flies and blow flies, and a pheromone attractant that attracts the insects. (Z)-9-tricosene is exemplified as this pheromone attractant. Furthermore, for example, Patent Document 3 discloses a composition for controlling fruit flies that contains 9-tricosene, which inhibits fruit flies mating by disrupting their mating. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] Japanese Patent Application Publication No. 2019-189559 [Patent Document 2] Special Publication No. 8-508287 [Patent Document 3] Patent No. 6992011 Summary of the Invention [Problem to be solved by the invention]

[0007] Secretions of certain insect species (e.g., pheromones and allomones) have the specificity of inducing a response in a specific insect species. Insect secretions have the advantage of having little effect on mammals, including humans, and birds. From this perspective, pest control agents that utilize insect secretions are useful for controlling pests that may exist indoors and outdoors in food and pharmaceutical factories.

[0008] The present disclosure has been made in consideration of the above circumstances, and its purpose is to provide a technology for selectively controlling specific pests by utilizing components contained in the secretions of insects that have little impact on mammals, including humans, and birds. [Means for solving the problem]

[0009] In order to solve the above problems, a pest control method according to one embodiment of the present disclosure includes: A control agent containing one or more hydrocarbons with a carbon number of 23 to 29 as a control ingredient is applied to an area to be controlled for one or more pests selected from the group consisting of slender planifera beetles, planifera beetles, serrated beetles, mealworm beetles, thrush beetles, bookworms, broad-leaved moths, wasps, pill bugs, woodlice, and treasure mites; The pest is controlled from the target area by activating a specific region of the antennal lobe of the pest and inducing an aversive behavior of the pest.

[0010] A pest control agent according to another embodiment of the present disclosure comprises: The control component for one or more pests selected from the group consisting of narrow-headed beetles, flathead beetles, silverfish, mealworms, dung beetles, bookworms, broad-headed moths, wasps, pill bugs, woodlice, and jewel mites contains one or more hydrocarbons with 23 to 29 carbon atoms that activate specific regions of the antennal lobes of the pests and induce aversive behavior in the pests.

[0011] A method for producing a pest control agent according to yet another embodiment of the present disclosure includes: A method for producing a pest control agent for controlling one or more pests selected from the group consisting of slender beetles, flat beetles, beetles, mealworms, larvae, bookworms, broad-leaved moths, wasps, pill bugs, woodlice, and treasure mites, The method includes a step of supporting, on a carrier, a control component containing one or more hydrocarbons having 23 to 29 carbon atoms that activate specific regions of the antennal lobes of the pest and induce aversive behavior in the pest. [Effects of the Invention]

[0012] According to the present disclosure, a technology can be provided for selectively controlling specific pests by utilizing components contained in the secretions of insects that have little impact on mammals, including humans, and birds. [Brief explanation of the drawings]

[0013] [Figure 1]FIG. 1 is a chart showing the test results of the control effect. DETAILED DESCRIPTION OF THE INVENTION

[0014] The pest control technology according to the present disclosure will be described in detail below. The sensory center of the insect brain includes the antennal lobe, which receives olfactory input. The antennal lobe has numerous glomeruli, and constitutes the primary center of the olfactory system, with glomeruli as functional units. It is known that in the insect brain, specific odors strongly activate glomeruli in specific regions of the antennal lobe, causing neural confusion and eliciting aversive behavior in the insect. Herein, "aversive behavior" refers to behavior induced by unpleasant stimuli or a motivation to escape from an object. The specific region of the antennal lobe in the insect brain that is strongly activated to elicit aversive behavior is hereinafter referred to as the "aversive region."

[0015] The pest control agent according to the present disclosure (hereinafter simply referred to as the control agent) contains a specific hydrocarbon as a control component, and this control component strongly activates the glomeruli in the aversive region of the antennal lobe of the pest, thereby inducing aversive behavior in the pest, thereby controlling the pest from the target control area.

[0016] In this specification, "control" mainly means "repellent." "Avoidance behavior" is the behavior of pests to avoid or move away from a control agent, and is one aspect of aversive behavior.

[0017] The pest control agents target one or more of the following pests: narrow-headed beetles, flathead beetles, beetles, mealworms, larvae, bookworms, hornworm moths, wasps, pill bugs, woodlice, and jewel mites. These pests may be found both indoors and outdoors in food and pharmaceutical factories, and are known as food pests (food pests include stored grain pests), pharmaceutical pests, or nuisance pests.

[0018] In a study titled "Chemical identification of an active component and putative neural mechanism for repellent effect of a native ant's odor on invasive species" (Tatsuya Uebi, Tomoya Sakita, Ryo Ikeda, Keita Sakanishi, Tomoaki Tsutsumi, Zijian Zhang, Huiying Ma, Ryosuke Matsubara, Shigeru Matsuyama, Satoko Nakajima, Rong-Nan Huang, Shunya Habe1, Abraham Hefetz7 and Mamiko Ozaki, published August 30, 2022), it was reported that specific hydrocarbon components in the cuticular hydrocarbons of the Japanese Camponotus japonica induce activation of glomeruli in the aversive region of the antennal lobe of Argentine ants, resulting in aversive behavior in the Argentine ants. According to this report, the hydrocarbons that induced aversive behavior were hydrocarbons with 23 to 29 carbon atoms. Hydrocarbons that were confirmed to induce aversive behaviors were branched alkanes including 7,15-dimethylheptacosane, 5,7,12-trimethylpentacosane, 7,9,12-trimethylpentacosane, and 5,7,12-trimethylheptacosane; (Z)-9-alkenes including (Z)-9-tricosene, (Z)-9-pentacosene, (Z)-9-hexacosene, (Z)-9-heptacosene, and (Z)-9-nonacosene; (Z)-7-alkenes including (Z)-7-tricosene, (Z)-7-pentacosene, and (Z)-7-hexacosene; 7-alkynes including 7-tricosine; and 9-alkynes including 9-tricosine and 9-pentacosine. Among these hydrocarbons, unsaturated hydrocarbons with 23 to 29 carbon atoms (i.e., alkynes with one carbon-carbon triple bond and alkenes with one carbon-carbon double bond) strongly activate the glomeruli in the aversive area of the antennal lobe of the Argentine ant. In particular, (Z)-9-tricosene, one of the (Z)-9-alkenes, strongly activates the glomeruli in the aversive area of the antennal lobe of the Argentine ant.

[0019] It was found that the target pests controlled by the control agent according to the present disclosure, like the Argentine ant, exhibited particularly strong aversive behavior toward (Z)-9-tricosene. From this, it can be inferred that the same types of hydrocarbons that induce aversive behavior in the Argentine ant also induce aversive behavior in the target pests controlled by the control agent according to the present disclosure.

[0020] In light of the above, the control component of the control agent contains one or more hydrocarbons having 23 to 29 carbon atoms.

[0021] The control component of the pesticide preferably contains one or more unsaturated hydrocarbons having 23 to 29 carbon atoms. Unsaturated hydrocarbons having 23 to 29 carbon atoms are surface hydrocarbons constantly secreted by certain insects, including the Japanese carpenter ant, and do not affect mammals, including humans, or birds. Therefore, the pesticide of the present disclosure is highly safe for humans. Furthermore, the control component of the pesticide of the present disclosure selectively acts on specific pests, and therefore has a smaller environmental impact than insecticides with indiscriminate toxicity, such as pyrethroid insecticides. Furthermore, the control component of the pesticide of the present disclosure is less likely to cause pests to develop resistance to the control component of the pesticide of the present disclosure compared to pyrethroid insecticides, and therefore the pesticide can be expected to be used for a long time. The unsaturated hydrocarbon may be extracted from the surface of an insect or may be chemically synthesized.

[0022] The control component of the control agent is preferably one or more of tricosene, pentacosene, and hexacosene.

[0023] The control component of the control agent is preferably one or more of the (Z)-9-alkenes (Z)-9-tricosene, (Z)-9-pentacosene, (Z)-9-hexacosene, (Z)-9-heptacosene, and (Z)-9-nonacosene.

[0024] Of the above (Z)-9-alkenes, (Z)-9-tricosene is a more preferred pest control component from the viewpoints of availability and economy. (Z)-9-tricosene is an unsaturated hydrocarbon with 23 carbon atoms, a molecular weight of 322.61, and the chemical formula CH3(CH2)7CH=CH(CH2). 12 CH3 has a melting point of 0°C, a boiling point of 300°C, a specific gravity (density) of 0.806 g / ml at 25°C, and is a colorless-yellow liquid at room temperature. In nature, (Z)-9-tricosene is a compound contained in the pheromones (i.e., body surface extracts) of spiders, ants, flies, and other insects. (Z)-9-tricosene also exists in nature as a communication pheromone for honeybees.

[0025] The control agent may contain other components in addition to the control component described above. Examples of other components include a base that dissolves the main unsaturated hydrocarbon, a carrier that supports the main component, and additives. Examples of additives include solvents, dispersants, emulsifiers, buffers, stabilizers, excipients, binders, lubricants, antioxidants, and preservatives, and the additives may be used alone or in combination of two or more.

[0026] The formulation of the control agent is not particularly limited. The control agent may be in a liquid or semi-solid form such as an oil solution, an emulsion, an aqueous emulsion, or a suspension. Alternatively, the control agent may be in a solid form such as a capsule, a powder, a granule, a tablet, a sheet, or a film. Alternatively, the control agent may be in a gaseous form when applied, such as an aerosol, a vaporization formulation, a resin vaporization agent, a fumigation agent, or a heat vaporization agent.

[0027] A liquid control agent may be the active ingredient of a solution of the control component, or may be obtained by diluting the active ingredient with a solvent, while a gel control agent may be obtained by dissolving the active ingredient of a solution of the control component in a base that adjusts viscosity.

[0028] A control method using a liquid or gel-type pesticide involves applying the pesticide to a target area by directly applying or spraying the pesticide to the target area, or by spraying the pesticide onto the target area using a sprayer. The target area includes walls, floors, ground, and ceilings that serve as entry routes for pests. The surface of the target area to which the pesticide has been applied, i.e., to which the pesticide has adhered, is referred to as the "application surface." A sufficient amount of the pesticide component is applied to the target area to cause pests to repellent.

[0029] A solid pesticide is formed by supporting a chemical solution containing a control component on a carrier. The chemical solution supported on the carrier may be the active ingredient of the chemical solution of the control component, or the active ingredient diluted with a solvent. A method for producing a solid pesticide includes a step of supporting the chemical solution on a carrier and a step of drying the chemical solution supported on the carrier. Examples of the carrier include porous bodies, glass beads, resin beads, films, sheets, tapes, cloth, pulp molded bodies, mesh, and powders. An appropriate carrier is adopted depending on the desired formulation of the pesticide. The method for supporting the chemical solution on the carrier is not particularly limited, and examples include a method of impregnating the carrier with the chemical solution, a method of coating the carrier with the chemical solution, and a method of spraying the chemical solution on the carrier.

[0030] A control method using a solid pesticide is a method of applying the pesticide to a target area by spraying the pesticide on the target area, attaching the pesticide to the target area, or covering the target area with the pesticide. The target area is a wall, floor, ceiling, ground, concrete surface, or the like that serves as an entry route for pests, and the solid pesticide is applied, i.e., placed, on these surfaces.

[0031] When the control ingredient of a liquid or gel pesticide is (Z)-9-tricosene, the amount of (Z)-9-tricosene to be applied varies depending on the target pest, but the amount of (Z)-9-tricosene to be applied is approximately 1 m 2 The amount of Z-9-tricosene applied is 0.0005 g or more, preferably 0.005 g or more, more preferably 0.05 g or more per 1 m of application surface. 2If the amount is less than 0.0005 g per square meter, the proportion of pests that do not show repellent behavior may increase. There is no upper limit to the amount of (Z)-9-tricosene that can be applied, but from an economical standpoint, it is recommended that the amount be less than 0.0005 g per square meter of the area to be controlled. 2 50g per serving is sufficient.

[0032] When the control ingredient of the solid control agent is (Z)-9-tricosene, the amount of (Z)-9-tricosene carried on the carrier is 1 m of the carrier surface (i.e., the surface of the carrier). 2 The amount of Z-9-tricosene supported is 0.0005 g or more, preferably 0.005 g or more, and more preferably 0.05 g or more per m of supporting surface. 2 If the amount is less than 0.0005 g per square meter, the proportion of pests that do not exhibit repellent behavior may increase. 2 It should be noted that the terms "application surface" and "carrier surface" refer to apparent areas, not surface areas that take into account irregularities present on the surface.

[0033] To pests, the control component of the control agent has an unpleasant odor, a frightening odor, or an aversive odor. When pests sense the odor of the control component through their antennae, the glomeruli in the aversive region of the antennal lobe are strongly activated, causing neural confusion and inducing aversive behavior in the pest. Pests that exhibit aversive behavior will avoid the surface to which the control agent has been applied, will not attempt to contact the surface, and will be unable to crawl or walk across the surface. Therefore, by applying the control agent to the pest's entry route into a factory building, pests attempting to crawl or walk into a factory can be controlled. Flying insects such as bees will avoid the surface to which the control agent has been applied, will not attempt to contact the surface, and will be unable to land on the surface. Therefore, by applying the control agent to a location on the entry route into a factory building where flying insects are likely to land, the invasion of flying insects can be suppressed.

[0034] It has been confirmed that the control component of the control agent has a lethal effect in addition to a repellent effect against slender beetles, beetles, mealworms, bookworms, and treasure mites. That is, the control agent can be used as an insecticide against slender beetles, beetles, mealworms, bookworms, and treasure mites. Bookworms and treasure mites are less sensitive to conventional pyrethroid insecticides, but are highly sensitive to the control agent of the present disclosure.

[0035] [Example] Hereinafter, the effects of the control agent and the control method using the control agent of the present disclosure will be specifically explained using test examples.

[0036] [Verification test of control effect 1] In this test, it was confirmed that the control agent and control method of the present disclosure have a repellent effect against slender planifer beetles, planifer beetles, beetles, mealworm beetles, thrush beetles, bookworms, hornworm moths, wasps, pill bugs, woodlouses, and treasure mites. The repellency test was conducted indoors and outdoors.

[0037] About pesticides The control agent used in the test contained (Z)-9-tricosene as the control ingredient. The control agent consisted of a (Z)-9-tricosene solution in its active ingredient form and a diluted solution prepared by diluting the active ingredient with acetone. The (Z)-9-tricosene solution was manufactured by Tokyo Chemical Industry Co., Ltd. (product name: cis-9-Tricosene) and had a purity of 98.0% or higher.

[0038] About the test insects For the tests, test insects prepared included slender beetles, flat beetles, beetles, mealworms, larvae, bookworms, tephritid moths, and treasure mites. Control test insects were also prepared for the control test. The control test insects were rice weevils, rice weevils, German cockroaches, bedbugs, and white-legged dermestids. The wasps, pill bugs, and woodlice were found in the outdoor test area. <Slim-headed aphids> The slender beetle belongs to the class Insecta, subclass Pterygota, order Coleoptera, and family Platyceridae / Plachyceridae. The slender beetle is a food pest that feeds on grains such as wheat flour, as well as sweets and dried fruit. The test insect used was the sawtooth beetle. <Flathead beetles> Flathead beetles belong to the class Insecta, subclass Pterygota, order Coleoptera, and family Pectinatidae. They are food pests that feed on grains such as wheat flour, as well as sweets and dried fruit. Flathead beetles were used as test insects. <Serif beetles> Lasioderma serricorne belongs to the class Insecta, subclass Pterygota, order Coleoptera, and family Anobiidae. Lasioderma serricorne is a food and pharmaceutical pest that feeds on processed foods such as grain flour, dried noodles, and sweets, as well as spices, dried fruit, dried sweet potatoes, dried foods, and herbal medicines. The test insects used were the cigarette beetle and the cigarette beetle. <Mealworms> Tenebrionid beetles belong to the class Insecta, subclass Pterygota, order Coleoptera, and family Tenebrionidae. Tenebrionid beetles are food pests that feed on secondary processed products such as grain flour, confectionery, and bread. The test insects used were the red flour beetle, the broad-headed red flour beetle, and the large-horned red flour beetle. <Shrimp beetles> The genus Pterygota belongs to the class Insecta, subclass Pterygota, order Coleoptera, and family Pterygonoidae. They are food and pharmaceutical pests that infest mold itself and moldy foods. The test insects used were the genus Pterygota and the genus Pterygonoida. <Pochonidae> Liposcelides belong to the class Insecta, subclass Pterygota, suborder Liposcelidae, and family Liposcelidae. Liposcelides are food and pharmaceutical pests that damage many foods, mainly grains and dried foods, as well as medicines and books. The test insect used was the flat-headed bookcelide. <Broad-winged moths> The tunic moths belong to the class Insecta, order Lepidoptera, and family Tunicidae. The tunicidae family includes food pests that feed on grains and processed foods. The test insect used was the carp moth. The carp moth is a fabric pest that feeds on fabrics. <Honeybees> They belong to the class Insecta, order Hymenoptera, and family Apidae. Honeybees are nuisance pests that can fly and invade food and pharmaceutical factories. Western honeybees were used as test insects. <Pill bugs> Pill bugs belong to the class Malacostraca, subclass Eumalacostraca, order Isopoda, and family Armadillididae. Pill bugs are nuisance pests and may inhabit the premises of food and pharmaceutical factories. Armadillidida were used as test insects. <Woodlouse> Woodlice belong to the class Malacostraca, subclass Eumalacostraca, order Isopoda, and family Isopidae. Woodlice are nuisance pests and may inhabit the premises of food and pharmaceutical factories. Woodlice were used as test insects. <Jewel mites> Takara mites belong to the class Arachnida, subclass Acari, order Acari, suborder Trigonotidae, and family Takara mite. Although Takara mites are not insects, they are included in the category of pests in this specification. Takara mites are nuisance pests and may inhabit the premises of food and pharmaceutical factories. The test insect used was the wall-attached Takara mite.

[0039] Indoor repellent test Test Method: (1) A 15 mm wide resin tape is attached to the bottom of a 90 mm diameter plastic dish to form a rectangular frame-shaped application surface, and a 1 m 250 ml of the control agent was applied per square meter. The rectangular frame represents the "treated area" where the agent was treated. Inside the rectangular frame is a 20 mm x 20 mm square injection stage. The control agents used were (Z)-9-tricosene active ingredient, a 10x diluted solution of the active ingredient diluted 10x, a 100x diluted solution of the active ingredient diluted 100x, a 1000x diluted solution of the active ingredient diluted 1000x, a 10000x diluted solution of the active ingredient diluted 10000x, a 10000x diluted solution of the active ingredient diluted 100000x, a 100000x diluted solution of the active ingredient diluted 100000x, and a 1000000x diluted solution of the active ingredient diluted 100000x. In this specification and claims, the dilution ratio of the active ingredient is a volume ratio. For an application surface of 1 m, 2 The application amount of (Z)-9-tricosene per dose is 0.00005g for a 1,000,000-fold diluted solution, 0.0005g for a 10,000-fold diluted solution, 0.005g for a 10,000-fold diluted solution, 0.05g for a 1,000-fold diluted solution, 0.5g for a 100-fold diluted solution, 5g for a 10-fold diluted solution, and 50g for the original drug. (2) One test insect was released into the input stage surrounded by the application surface, and if the test insect was inside the rectangular frame after a specified time had passed, it was judged to have "repelled," and if it was outside the rectangular frame, it was judged to have "not repelled." This was repeated the total number of individuals released, and the number of repelled individuals was counted. Individuals present outside the rectangular frame were deemed to have walked beyond the rectangular frame from the input stage without being repelled by the pesticide.

[0040] Evaluation Method: The repellency rate was calculated using the following formula 1. Repellency rate [%] = number of repelled individuals / number of introduced individuals × 100 (Equation 1) Indoor repellent test results, i.e., targeting multiple types of test insects, application surface 1m 2 The following Table 1 shows the change in repellency rate depending on the amount of control ingredient applied per unit area.

[0041] [Table 1]

[0042] As shown in Table 1, test insect numbers 1-12 exhibited repellent behavior toward the pesticide. On the other hand, control test insect numbers C1-C5 did not exhibit repellent behavior toward the pesticide. Thus, it is clear that the pesticide induces repellent behavior toward certain pests.

[0043] The susceptibility of the test insects Nos. 1-12 to (Z)-9-tricosene varies. From the test results shown in Table 1, the amount of (Z)-9-tricosene applied to the pests to achieve a repellent effect is 1000 mg / m². 2 0.0005g or more per unit area, and for pea beetles, the application area is 1m 2 0.05g or more per unit area, and for beetles and mites, the application area is 1m 2 0.5g or more per unit area, and for slender beetles and mealworms, the application area is 1m 2 5g or more per unit area, and for broad-leaved moths and flathead beetles, the application area is 1m 2 In this case, the repellency rate was found to be 50% or more, which means that the product was deemed to have a sufficient repellent effect against pests.

[0044] Outdoor repellency test Test Method: (1) 1 m of Z-9-tricosene raw material on a 15 mm wide resin tape 2 The resin tape was applied to the entrance of the insect habitat survey trap, and this trap was designated as the "treated area." Resin tape without Z-9-tricosene was applied to the entrance of the trap, and this trap was designated as the "control area." (2) The treated and control traps were left in the outdoor test area for 24 hours, and the organisms captured in each trap (hereinafter referred to as captured organisms) were counted. The test was repeated twice, with the locations of the treated and control traps interchanged.

[0045] Evaluation Method: The repellency rate for each type of captured organism was calculated using the following formula 2. Repellency rate [%] = (total number of captured organisms in the control area - total number of captured organisms in the treatment area) / total number of captured organisms in the control area × 100 (Equation 2) The test results of the outdoor repellency test, i.e., the repellency rate, are shown in Table 2 below.

[0046] [Table 2]

[0047] As shown in Table 2, organisms numbered 13-14 (i.e., mites, pill bugs, and woodlice) exhibited repellent behavior toward the pesticide. On the other hand, organisms numbered C7-C12 did not exhibit repellent behavior toward the pesticide, but rather were attracted to it. Thus, it is clear that the pesticide causes repellent behavior toward certain organisms.

[0048] [Verification test of insecticidal effect] Tests have confirmed that the control agent and control method of the present disclosure have insecticidal effects against slender beetles, serrated beetles, mealworms, bookclops, and treasure mites. The insecticidal tests were conducted using a clip test and a dry film test. The control agent used in the insecticidal test was (Z)-9-tricosene, just like in the repellency test.

[0049] Insecticidal test: Clip test method Test Method: The clip test method was adopted and the test was carried out according to the following procedure. (1) Filter paper cut to a size of 10 cm long x 5 cm wide was prepared. (2) 0.25 mL (50 mL / m) of each of several concentrations of the drug solution obtained by diluting the original substance with a solvent and the drug solution obtained by diluting the solvent with water or the like to a predetermined concentration was added. 2 The solution was dripped evenly onto filter paper and stored indoors to allow the solvent to evaporate. The chemicals used were (Z)-9-tricosene, its active ingredient, a 10x diluted solution of the active ingredient, a 100x diluted solution of the active ingredient, a 1000x diluted solution of the active ingredient, a 1000x diluted solution of the active ingredient, and a 10000x diluted solution of the active ingredient. (3) The filter paper was folded in half and the two sides were fastened together with eye clips. 20-30 test insects were placed into one of the open openings, and the other side was fastened with a clip to seal the opening. (3) After 24 hours at 25°C, the clips were removed from the filter paper and the test insects were observed for survival.

[0050] Evaluation Method: The mortality rate was calculated using the following formula 3 for each of the filter paper soaked in the pesticide (treated area) and the filter paper soaked in only the solution (control area). Mortality rate [%] = number of dead insects / number of introduced insects × 100 (Equation 3) Furthermore, the corrected mortality rate was calculated using the following formula 4. Corrected insect mortality rate [%] = (mortality rate in treated area - mortality rate in control area) / (100 - mortality rate in control area) (Equation 4) The test results of the insecticidal test, i.e., the corrected insect mortality rate, are shown in Table 3 below.

[0051] [Table 3]

[0052] Insecticide test: Dry film test method Test Method: The dry film test method was adopted and the test was carried out according to the following procedure. (1) The pesticide was attached to the bottom wall of a glass petri dish. (2) Test insects were placed in a petri dish and allowed to come into contact with the pesticide. (3) After 24 hours, the number of surviving insects and dead insects were determined.

[0053] Evaluation Method: The survival rate was calculated using Equation 5 for the bottom wall of the petri dish to which the pesticide had been applied (treated group) and the bottom wall of the petri dish to which no pesticide had been applied (control group). Survival rate [%] = number of surviving individuals / number of individuals introduced × 100 (Equation 5) Furthermore, the corrected mortality rate was calculated by applying the obtained survival rate to the following formula 6 (Abbott's correction formula). Corrected mortality rate [%] = (survival rate in control group - survival rate in treatment group) / (survival rate in control group) × 100 (Equation 6) The test results of the insecticidal test, i.e., the corrected insect mortality rate, are shown in Table 4 below.

[0054] [Table 4]

[0055] As shown in Tables 3 and 4, it is clear that (Z)-9-tricosene has an insecticidal effect against pests Nos. 15-20 (i.e., slender beetles, serpent beetles, mealworms, bookworms, and treasure mites). Furthermore, the application amount (i.e., lethal dose) of (Z)-9-tricosene that exhibits an insecticidal effect against these pests is 1 / m of the application surface (or carrying surface). 2 The weight of each beetle was found to be over 0.5g for bookworms, and over 5g for slender beetles, silverfish, mealworms, and jewel mites.

[0056] [Verification test of control effect 2] In this test, we investigated the difference in repellency effect depending on the control ingredient of the pesticide. We prepared pesticides with different control ingredients: (Z)-9-tricosene, (Z)-9-pentacosene, and (Z)-9-hexacosene, and conducted indoor and outdoor repellency tests.

[0057] Indoor repellent test Test Method: The test method for the indoor repellent test in "Verification Test 1 of Control Effect" above is cited by reference. The test insect was the flat-headed bookworm. 2 The appropriate amounts of the control components per unit area were 0.005 g for (Z)-9-tricosene, 0.005 g for (Z)-9-pentacosene, and 0.005 g for (Z)-9-hexacosene. Evaluation Method: The evaluation method for the indoor repellency test in "Verification Test 1 of Control Effect" above is cited by reference. The test results of the indoor repellency test, i.e., the difference in repellency rate due to the difference in control ingredients using the flat-headed bookworm as the test insect, are shown in Table 5 below.

[0058] [Table 5]

[0059] As shown in Table 5, the control ingredients (Z)-9-tricosene, (Z)-9-pentacosene, and (Z)-9-hexacosene were confirmed to have a repellent effect against the flat-headed book lice. Furthermore, although there were differences in the repellent effects of (Z)-9-tricosene, (Z)-9-pentacosene, and (Z)-9-hexacosene against the flat-headed book lice, it was found that the differences were not significant.

[0060] Outdoor repellency test Test Method: (1) 1m of the active ingredient of the pesticide is applied to a 15mm wide resin tape. 2 The resin tape was applied to the entrance of the trap used for the insect habitat survey, and this trap was designated as a treatment trap. In addition, resin tape without any pesticide was applied to the entrance of the trap, and this trap was designated as a control trap. (2) The treated and control traps were left in the outdoor test area for 24 hours, and the number of wall mites (trapped organisms) captured in each trap was counted. The test was repeated a total of six times. Test results: The test results are shown in Figure 1. As shown in Figure 1, almost no wall mites were captured in the traps treated with (Z)-9-tricosene, (Z)-9-pentacosene, and (Z)-9-hexacosene. On the other hand, more wall mites were captured in the control traps than in the treated traps. This confirmed the repellent effect of each of the control ingredients, (Z)-9-tricosene, (Z)-9-pentacosene, and (Z)-9-hexacosene, against wall mites. Furthermore, in the treated traps, there was almost no difference in repellent effect due to the difference in control ingredients. This indicates that (Z)-9-tricosene, (Z)-9-pentacosene, and (Z)-9-hexacosene have equivalent repellent effects against wall mites.

[0061] [Summary] The pest control method according to the first aspect of the present disclosure includes: A control agent containing one or more hydrocarbons with a carbon number of 23 to 29 as a control ingredient is applied to an area to be controlled for one or more pests selected from the group consisting of slender planifera beetles, planifera beetles, serrated beetles, mealworm beetles, thrush beetles, bookworms, broad-leaved moths, wasps, pill bugs, woodlice, and treasure mites; The pest is controlled from the target area by activating a specific region of the antennal lobe of the pest and inducing an aversive behavior of the pest.

[0062] A pest control method according to a second aspect of the present disclosure is the pest control method according to the first aspect, wherein the control component is one or more unsaturated hydrocarbons having 23 to 29 carbon atoms.

[0063] A pest control method according to a third aspect of the present disclosure is the pest control method according to the first or second aspect, wherein the control component is one or more of tricosene, pentacosene, and hexacosene.

[0064] The pest control method according to the fourth item of the present disclosure is the pest control method according to the first or second item, wherein the control component is one or more of the (Z)-9-alkenes (Z)-9-tricosene, (Z)-9-pentacosene, (Z)-9-hexacosene, (Z)-9-heptacosene, and (Z)-9-nonacosene.

[0065] The pest control method according to the fifth item of the present disclosure is the pest control method according to the first item, wherein the control component is (Z)-9-tricosene and the amount of (Z)-9-tricosene applied is 0.0005 g or more per m2 of application surface.

[0066] The pest control method according to the sixth item of the present disclosure is a pest control method according to any one of the first to fifth items, in which the control component is applied to an area to be controlled for one or more pests selected from the group consisting of bookclops and mites in an amount that is lethal to the pests.

[0067] The pest control agent according to the seventh item of the present disclosure contains, as a control component for one or more pests selected from the group consisting of narrow-headed beetles, flathead beetles, beetles, mealworms, dung beetles, bookworms, broad-headed moths, wasps, pill bugs, woodlice, and jewel mites, one or more hydrocarbons having 23 to 29 carbon atoms that activate specific regions of the antennal lobes of the pests and induce aversive behavior in the pests.

[0068] The eighth aspect of the present disclosure relates to a method for producing a pest control agent for controlling one or more pests selected from the group consisting of narrow-headed beetles, flathead beetles, beetles, mealworms, dung beetles, bookworms, hornworms, wasps, pill bugs, woodlice, and treasure mites, and the method includes a step of supporting on a carrier a control component containing one or more hydrocarbons having 23 to 29 carbon atoms that activate specific regions of the antennal lobes of the pests and induce aversive behavior in the pests.

Claims

1. A control agent containing one or more hydrocarbons having a carbon number of 23 to 29 as a control ingredient is applied to an area to be controlled for one or more pests selected from the group consisting of slender planifera beetles, planifera beetles, serrated beetles, mealworm beetles, thrush beetles, bookworms, hornworm moths, wasps, pill bugs, woodlice, and treasure mites; The pest is controlled from the control target location by activating a specific region of the antennal lobe of the pest to induce an aversive behavior of the pest. Pest control methods.

2. The control component is one or more unsaturated hydrocarbons having 23 to 29 carbon atoms. The method for controlling pests according to claim 1.

3. The control component is one or more of tricosene, pentacosene, and hexacosene. The method for controlling pests according to claim 1.

4. The control component is one or more of (Z)-9-alkenes selected from (Z)-9-tricosene, (Z)-9-pentacosene, (Z)-9-hexacosene, (Z)-9-heptacosene, and (Z)-9-nonacosene; The method for controlling pests according to claim 1.

5. The control component is (Z)-9-tricosene, and the amount of (Z)-9-tricosene applied is 1 m ２ 0.0005g or more per The method for controlling pests according to claim 1.

6. Applying the control component to an area to be controlled that is one or more pests selected from the group consisting of bookclops and mites in an amount that is lethal to the pests. The method for controlling pests according to any one of claims 1 to 5.

7. The control component for one or more pests selected from the group consisting of slender planifolia beetles, planifolia beetles, beetles, mealworms, beetles, bookworms, hornworm moths, wasps, pill bugs, woodlouses, and jewel mites contains one or more hydrocarbons having 23 to 29 carbon atoms that activate specific regions of the antennal lobes of the pests to induce aversive behavior in the pests. Pest control agent.

8. A method for producing a control agent for controlling one or more pests selected from the group consisting of slender planifolia, planifolia, beetles, mealworms, larvae, bookworms, hornworms, wasps, pill bugs, woodlouses, and treasure mites, the method comprising the step of supporting on a carrier a control component containing one or more hydrocarbons having 23 to 29 carbon atoms that activate specific regions of the antennal lobes of the pests to induce aversive behavior in the pests. Method for manufacturing pest control agents.

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