Gene delivery system and application thereof in preparation of drugs for treatment of tumors

Abstract

To suppress growth of tumor cells and provide novel means for tumor treatment by inducing differentiation of tumor cells into mature cells by mediating overexpression of HNF4α in tumor cells by self-replicating RNA.SOLUTION: Provided is a gene delivery system comprising a nucleic acid vector and a delivery vehicle. Therein the nucleic acid vector is a self-replicating RNA carrying HNF4α; the gene delivery system delivers HNF4α into tumor cells and induces differentiation of the tumor cells into normal mature cells; the self-replicating RNA is based on one engineered alphavirus genome and has a polyadenylic acid tail of 35-100 nt; and the delivery vehicle is a lipid nanoparticle.SELECTED DRAWING: Figure 23

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