Composition for removing heavy metals containing probiotics

Abstract

The present invention relates to a composition for removing heavy metals, a pharmaceutical composition, a food composition or a pharmaceutical composition comprising one or more lactic acid bacteria selected from the group consisting of bacteria of the genus Lactobacillus, bacteria of the genus Bifidobacterium, bacteria of the genus Enterococcus, bacteria of the genus Pediococcus and bacteria of the genus Streptococcus. The composition for removing heavy metals according to the present invention has excellent resistance to heavy metals, antibacterial effect and heavy metal excretion, and has the advantage of being effectively used for removing heavy metals by excreting heavy metals from the living body.

Description

[Technical field]

[0001] The present invention relates to a composition for removing heavy metals that contains probiotics. [Background technology]

[0002] With the development of society, technology and civilization, the severity of environmental pollution has been caused by various factors. Among them, heavy metals discharged during the process of production activities are highly dangerous to the human body and also pose a threat to the ecosystem. The human body, which is thus susceptible to risk, is exposed to the surrounding environment such as food, water, air and soil, and is constantly ingesting heavy metals, although to some extent this varies from person to person.

[0003] In particular, the amount of harmful heavy metals that Koreans ingest through food is close to the ADI (acceptable daily intake) of the human body, and once these heavy metals enter the human body, they cannot be easily excreted. Among these, toxic heavy metals react with human tissues even at relatively low concentrations and gradually become toxic. Once a certain level is exceeded, recovery or cure is impossible, which is a major problem in the modern food system.

[0004] Cadmium (Cd), one of the heavy metals that are toxic to the human body, is found in relatively low concentrations in nature but is extremely harmful, and its use in various factories and industrial sites is increasing. As a result of evaluating its effects on human health, it was found to cause acute and chronic poisoning, and interest in it as an environmental pollutant is growing. As such, research results have been reported that show that probiotics can reduce the absorption rate of cadmium, which is seriously damaging to the human body.

[0005] It is generally known that heavy metals are absorbed by microorganisms in two ways: first, by adsorption to the cell surface through the binding of cations to anionic groups present on the cell surface, and second, by a process of accumulation within the cells through metabolic action. Currently, research on cell surface adsorption is being conducted mainly.

[0006] Since research into the heavy metal excretion effect of lactic acid bacteria is currently insufficient, the inventors have completed the present invention by selecting bacterial strains with excellent heavy metal excretion ability based on their functions of acid resistance to probiotics and bile acid resistance. Summary of the Invention [Problem to be solved by the invention]

[0007] The present inventors have carried out studies to develop probiotic strains that can be applied for the adsorption or removal of heavy metals.

[0008] An object of the present invention is to provide a composition for removing heavy metals, which contains one or more lactic acid bacteria selected from the group consisting of Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus.

[0009] Another object of the present invention is to provide a cosmetic composition for removing heavy metals, which contains one or more lactic acid bacteria selected from the group consisting of Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus.

[0010] Another object of the present invention is to provide a pharmaceutical composition for removing heavy metals, comprising one or more lactic acid bacteria selected from the group consisting of Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus.

[0011] Yet another object of the present invention is to provide a food composition for removing heavy metals, comprising one or more lactic acid bacteria selected from the group consisting of Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus.

[0012] Other objects and technical features of the present invention will be more specifically presented in the following description of the invention, the claims and the drawings. [Means for solving the problem]

[0013] One aspect of the present invention relates to a composition for removing heavy metals, comprising one or more types of lactic acid bacteria selected from the group consisting of bacteria of the genus Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus.

[0014] In the present invention, the lactic acid bacteria may be one or more types of lactic acid bacteria selected from the group consisting of bacteria of the genus Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus.

[0015] The lactic acid bacteria of the present invention may be probiotic lactic acid bacteria in the sense that, when administered to the body, they colonize the intestine and exert a beneficial synergistic effect together with the gut microbiota.

[0016] According to one embodiment of the present invention, the Lactobacillus lactic acid bacteria may be one or more lactic acid bacteria selected from the group consisting of Lactobacillus acidophilus, Lactobacillus gasser, Lactobacillus lactis, Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus fermentum, and Lactobacillus plantarum.

[0017] According to one embodiment of the present invention, the Bifidobacterium lactic acid bacteria may be one type of lactic acid bacteria selected from the group consisting of Bifidobacterium infantis, Bifidobacterium bifidum, Bifidobacterium lactis, Bifidobacterium longum, and Bifidobacterium breve.

[0018] According to one embodiment of the present invention, the Enterococcus bacteria may be one or more lactic acid bacteria selected from the group consisting of Enterococcus faecium and Enterococcus faecalis.

[0019] According to one embodiment of the present invention, the Pediococcus bacterium may be a lactic acid bacterium, Pediococcus pentosaceus.

[0020] According to one embodiment of the present invention, the Streptococcus may be a lactic acid bacterium, Streptococcus thermophilus.

[0021] The composition for removing heavy metals in the present invention may have resistance to heavy metals. For example, the resistance may be one or more properties selected from minimum inhibitory concentrations (MICs), bacterial growth, and heavy metal removal or binding capacity.

[0022] The composition for removing heavy metals of the present invention functions to promote the excretion of heavy metals, and therefore, the composition for removing heavy metals of the present invention functions to excrete heavy metals accumulated in blood and tissues from the body.

[0023] The heavy metal in the present invention may be a cationic heavy metal or an anionic heavy metal. For example, the cationic heavy metal may be cadmium (Cd), copper (Cu), lead (Pb) or zinc (Zn), and the anionic heavy metal may be arsenic (As), chromium (Cr), selenium (Se) or antimony (Sb).

[0024] The cationic heavy metal in the present invention may be cadmium, and the bacteria may have a cadmium removal capacity of 22% or more in a 100 mg / L cadmium solution. Desirably, the bacteria may have a cadmium removal capacity of 29% or more in a 50 mg / L cadmium solution. More desirably, the bacteria may have a cadmium removal capacity of 51% or more in a 5 mg / L cadmium solution.

[0025] The cationic heavy metal in the present invention may be lead, and the bacteria may have a lead removal capacity of 22% or more in a 100 mg / L lead solution. Desirably, the bacteria may have a lead removal capacity of 41% or more in a 50 mg / L lead solution. More desirably, the bacteria may have a lead removal capacity of 76% or more in a 5 mg / L lead solution.

[0026] Another aspect of the present invention provides a pharmaceutical composition for preventing or treating heavy metal poisoning, comprising one or more lactic acid bacteria selected from the group consisting of Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus.

[0027] In the pharmaceutical composition of the present invention, the details regarding the Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus bacteria are the same as those explained in the "Composition for Removing Heavy Metals," another embodiment of the present invention described above, and therefore, these are incorporated herein and redundant explanations will be omitted.

[0028] The term "prevention" in this invention means inhibiting the occurrence of a disease, illness or condition in an animal that has not been diagnosed as having the disease, illness or condition but is predisposed to such disease, illness or condition.

[0029] The term "treatment" as used herein means (i) inhibiting the progression of a disease, disorder or condition; (ii) alleviating a disease, disorder or condition; or (iii) eliminating a disease, disorder or condition.

[0030] The pharma- ceutically acceptable carriers contained in the pharmaceutical composition of the present invention are those commonly used in formulation, and include, but are not limited to, carbohydrate compounds (e.g., lactose, amylose, dextrose, sucrose, sorbitol, mannitol, starch, cellulose, etc.), gum acacia, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, salt solution, alcohol, gum arabic, vegetable oils (e.g., corn oil, cottonseed oil, soybean oil, olive oil, coconut oil), polyethylene glycol, methylcellulose, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate, and mineral oil.

[0031] In addition to the above-mentioned components, the pharmaceutical composition of the present invention may further contain lubricants, wetting agents, sweeteners, flavoring agents, emulsifiers, suspending agents, preservatives, etc. Suitable pharma- ceutically acceptable carriers and formulations are described in detail in Remington's Pharmaceutical Sciences (19th ed., 1995).

[0032] The appropriate dosage of the pharmaceutical composition of the present invention may be variously prescribed depending on factors such as the formulation method, administration method, age, body weight, sex, medical condition, food, administration time, administration route, excretion rate, reaction sensitivity, etc. Meanwhile, the oral dosage of the pharmaceutical composition of the present invention is preferably 0.001 to 1000 mg / kg (body weight) per day.

[0033] The pharmaceutical composition of the present invention may be prepared in the form of a unit dose or in a multi-dose container by formulating it with pharma- ceutically acceptable carriers and / or excipients in a manner that can be easily carried out by a person having ordinary skill in the art to which the invention pertains. In this case, the dosage form may be in the form of a solution, suspension or emulsion in an oily or aqueous medium, or in the form of an extract, powder, granules, tablets or capsules, and may further include a dispersant or stabilizer.

[0034] Yet another aspect of the present invention provides a food composition comprising one or more lactic acid bacteria selected from the group consisting of Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus.

[0035] In the food composition of the present invention, the details regarding the Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus bacteria are the same as those explained in the "Composition for Removing Heavy Metals," another embodiment of the present invention described above, and therefore, these are incorporated herein and overlapping explanations will be omitted.

[0036] The food compositions of the present invention can be prepared into, but are not limited to, functional foods, nutritional supplements, health foods, or food additives.

[0037] The food composition of the present invention can be prepared in various forms by conventional methods known in the art, for example, as a beverage such as fermented milk, or as a powder. The food composition of the present invention contains not only the above-mentioned active ingredient of the present invention, but also ingredients that are usually added during the production of food, such as proteins, carbohydrates, fats, nutrients, seasonings and flavorings. Examples of the above-mentioned carbohydrates include common sugars such as monosaccharides such as glucose and fructose, disaccharides such as maltose, sucrose, and oligosaccharides, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavorings, natural flavorings [thaumatin, stevia extract (e.g., rebaudioside A, glycylrhizin, etc.)] and synthetic flavorings (saccharin, aspartame, etc.) can be used.

[0038] When the food composition of the present invention is produced in the form of a beverage, it may further contain, in addition to the active ingredient of the present invention, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, Tokiwa extract, jujube extract, licorice extract, etc.

[0039] Yet another aspect of the present invention provides a cosmetic composition comprising one or more lactic acid bacteria selected from the group consisting of bacteria of the genus Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus.

[0040] In the cosmetic composition of the present invention, the details regarding the Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus bacteria are the same as those explained in the "Composition for Removing Heavy Metals," another embodiment of the present invention described above, and therefore, these are incorporated herein and overlapping explanations will be omitted.

[0041] The components contained in the cosmetic composition of the present invention may include, as active ingredients, ingredients that are commonly used in cosmetic compositions in addition to the above-mentioned active ingredients, and include, for example, common adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances, and carriers.

[0042] The cosmetic composition of the present invention can be prepared into any formulation commonly used in the art, including, but not limited to, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, packs, massage creams, and sprays. More specifically, the cosmetic composition of the present invention can be prepared into the formulations of softening lotions, nourishing lotions, nourishing creams, massage creams, essences, eye creams, cleansing creams, cleansing foams, cleansing waters, packs, sprays, or powders.

[0043] When the cosmetic composition of the present invention is in the form of a paste, cream or gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, tracant, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, a silica, a talc or a zinc oxide.

[0044] When the cosmetic composition of the present invention is in the form of a solution or emulsion, a solvent, solubilizer, or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol fatty acid esters, polyethylene glycol, or fatty acid esters of sorbitan.

[0045] When the cosmetic composition of the present invention is in the form of a suspension, the carrier component may be a liquid diluent such as water, ethanol, or propylene glycol; a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene sorbitan ester; microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant.

[0046] When the cosmetic composition of the present invention is in the form of a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane / butane or dimethyl ether may be further included.

[0047] When the cosmetic composition of the present invention is in the form of a cleansing agent containing a surfactant, the carrier component may be, for example, a fatty alcohol sulfate, a fatty alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyl taurate, a sarcosinate, a fatty acid amide ether sulfate, an alkylamido betaine, a fatty alcohol, a fatty acid glyceride, a fatty acid diethanolamide, a vegetable oil, a lanolin derivative, or an ethoxylated glycerol fatty acid ester.

[0048] When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing preparation, or a surfactant-free cleansing preparation, it may be applied to the skin and then wiped off, removed, or washed off with water. Specific examples of the soap include liquid soap, powder soap, solid soap, and oil soap, surfactant-containing cleansing preparations include cleansing foam, cleansing water, cleansing towel, and cleansing pack, and surfactant-free cleansing preparations include cleansing cream, cleansing lotion, cleansing water, and cleansing gel, but are not limited thereto. Effect of the Invention

[0049] The present invention relates to a composition, pharmaceutical composition, food composition or pharmaceutical composition for removing heavy metals, which comprises one or more lactic acid bacteria selected from the group consisting of Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus and Streptococcus. The composition for removing heavy metals according to the present invention has excellent resistance to heavy metals, antibacterial effect and heavy metal excretion, and can be effectively used to excrete heavy metals from the living body and remove heavy metals. [Brief description of the drawings]

[0050] [Figure 1a] The results of the cadmium (Cd) MIC test and growth curve for LA1 and LGA are shown. [Figure 1b]The results of the cadmium (Cd) MIC test and growth curve for EF4 and EFL2 are shown. [Figure 1c] The results of the cadmium (Cd) MIC test and growth curve for SL6 and LPC5 are shown. [Figure 1d] The results of the cadmium (Cd) MIC test and growth curve for LH5 and LU4 are shown. [Figure 1e] The results of the cadmium (Cd) MIC test and growth curve for LR5 and LC5 are shown. [Figure 1f] The results of the cadmium (Cd) MIC test and growth curve for PP1, SL4 and LF2 are shown. [Figure 1g] The results of the cadmium (Cd) MIC test and growth curve for LP3 and BT1 are shown. [Figure 1h] The results of the cadmium (Cd) MIC test and growth curve for BF3 and BL3 are shown. [Figure 1i] The results of the cadmium (Cd) MIC test and growth curve for BG7 and BR3 are shown. [Figure 1j] The results of the cadmium (Cd) MIC test and growth curve for ST3 are shown. [Diagram 2] The cadmium (Cd) removal capacity (%) and p-value of each strain in a cadmium solution of 50 mg / L are shown. [Diagram 3] The cadmium (Cd) removal capacity (%) and p-value of each strain in a cadmium solution of 5 mg / L are shown. [Figure 4] The cadmium (Cd) removal capacity (%) and p-value of each strain in a cadmium solution of 100 mg / L are shown. [Figure 5a]The results of the lead (Pb) MIC test and growth for 11 strains other than LA1 are shown. [Figure 5b] The results of the lead (Pb) MIC test and growth for six strains other than LP3 are shown. [Figure 5c] The graph shows the change in lead (Pb) concentration for the 10 strains other than LA1. [Figure 5d] The graph shows the change in lead (Pb) concentration for the seven strains other than BL3. [Figure 6] The removal capacity (%) of lead (Pb) of each strain in a lead solution of 50 mg / L is shown. [Figure 7] The removal capacity (%) of lead (Pb) of each strain in a lead solution of 5 mg / L is shown. [Figure 8] The removal capacity (%) of lead (Pb) of each strain in a lead solution of 100 mg / L is shown. [Figure 9] SEM images of samples containing Lactobacillus plantarum and Lactobacillus acidophilus in 100 mg / L cadmium solution and 100 mg / L lead solution are shown. [Figure 10] FIG. 1 is a schematic diagram showing the process of selecting a bacterial strain having excellent cadmium removal ability. [Figure 11] 1 shows changes in body weight and liver weight due to the probiotics of the present invention. [Figure 12] 1 shows changes in excretion of cadmium (Cd) and lead (Pb) into feces by the probiotics of the present invention. [Figure 13] 1 shows changes in cadmium (Cd) and lead (Pb) contents in blood and tissues (liver, kidney) by the probiotic of the present invention. [Figure 14] 1 shows hematological changes in cadmium (Cd) and lead (Pb) by the probiotic of the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0051] The following describes preferred embodiments of the present invention. However, the embodiments of the present invention can be modified into various other forms, and the scope of the present invention is not limited to the embodiments described below. Furthermore, the embodiments of the present invention are provided to more completely explain the present invention to those having average knowledge in the art.

[0052] Experimental Method Experimental Example 1. Screening of probiotics with heavy metal removal ability In order to select probiotic strains with the ability to adsorb or remove heavy metals, in vitro screening was performed.

[0053] The genus Lactobacillus, the genus Bifidobacterium, the genus Enterococcus, the genus Pediococcus, and the genus Streptococcus were set as probiotic strains, and the genus Lactobacillus included Lactobacillus acidophilus, Lactobacillus gasser, Lactobacillus lactis, Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus fermentum, and Lactobacillus spp. As the genus Bifidobacterium, Bifidobacterium infantis, Bifidobacterium bifidum, Bifidobacterium lactis, Bifidobacterium longum, and Bifidobacterium breve were selected. As the genus Enterococcus, Enterococcus faecium and Enterococcus faecalis were selected. The tests were performed using Pediococcus faecalis as a Pediococcus species, Pediococcus pentosaceus as a Pediococcus species, and Streptococcus thermophilus as a Streptococcus species.

[0054] Experimental Example 2. Antibacterial Effect Measurement (MIC Test) For each of the strains selected in Experimental Example 1, antibacterial activity tests against heavy metals (MIC tests) were conducted to select the strains and evaluate their efficacy (in vitro). First, the strains were seeded on MRS or BL agar plates using sterilized paper disks. For antibacterial activity experiments, strains resistant to cadmium (Cd, concentration range: 50mg / L-1000mg / L, 50μl / disk) and lead (Pb, concentration range: 50mg / L-5000mg / L, 50μl / disk) were selected from the heavy metals. In addition, strains that adhere to cadmium or lead were selected using a binding capacity assay.

[0055] Experimental example 3. Bacterial growth test A bacterial growth test for heavy metals was used to screen the strains selected in Experimental Example 1 and evaluate their efficacy (in vitro). Cadmium and lead were administered to each strain, and the change in growth depending on the concentration of cadmium and lead was measured (see FIG. 10).

[0056] The cadmium concentration ranges were set to 0 mg / L, 100 mg / L, 500 mg / L, and 1000 mg / L, which are concentrations based on the results of the antibacterial effect evaluation (MIC test) in Experimental Example 1, and the growth patterns of each strain in MRS or BL broth were analyzed according to the set cadmium concentrations. The cadmium concentration ranges were set to 0 mg / L, 50 mg / L, 100 mg / L, 500 mg / L, 1000 mg / L, 2000 mg / L, 3000 mg / L, 4000 mg / L, and 5000 mg / L, which are concentrations based on the results of the antibacterial effect evaluation (MIC test) in Experimental Example 1.

[0057] The evaluation values ​​for cadmium and lead refer to the reconfirmation of the growth of each strain on MRS or BL broth in relation to the results of the antibacterial effect evaluation (MIC test) in Experimental Example 2.

[0058] Experimental Example 4. Heavy metal removal and binding capacity assay The heavy metal removal ability of each strain selected in Experimental Example 1 was examined. First, each strain was cultured for 18 hours, and the pellets were cultured in 1 mg / L (1 ppm) cadmium solution media at 37°C for 2 hours. Then, the cadmium concentration in the cadmium solution media was measured, and the MRE (Metal Removal Efficiency) or Cd uptake was calculated using the following formula.

[0059] MRE=C i -C f / (t f -t i )M(C i =initial Cd concentration, C f =final Cd concentration, t i = initial time, t f =final time, M=mass of cell, μg h -1 mg -1 ) Cd uptake=(C0-C1)V / m(C0(mg / L)=initial Cd concentration, C1(mg / L)=Cd concentration after adsorption, V(L)=volume of the solution, m(g)=amount of biomass) In addition, the cadmium binding characterization and lead binding characterization of each of the strains were analyzed.

[0060] To analyze cadmium binding properties, each strain was cultured for 18 hours, and the pellet was resuspended in 50 mg / L cadmium solution, and then adjusted to pH 6 with NaOH or HNO3 solution. After incubation at 37°C for 1 hour, the cadmium concentration in the supernatant was measured to select strains with excellent cadmium removal and binding properties. The lead binding properties were analyzed in the same manner as in the cadmium binding properties analysis, and the lead concentration was measured and evaluated.

[0061] Quantitative analysis of cadmium and lead was performed using an atomic absorption spectrometer, and cadmium and lead attached to the surface of the final selected strain were measured using a scanning electron microscope (SEM).

[0062] Experimental example 5. Animal experiment measurement (In vivo test) The probiotic strain of Experimental Example 1 was evaluated in an animal model (in vivo). The experimental animals were SD male rats, six per group. The rats were dosed with CdCl2 (cadmium chloride, Cd), Pb(C2H3O2)2 (lead acetate, Pb), and probiotics (LA+LP, Pro). CdCl2 (cadmium chloride, Cd), Pb(C2H3O2)2 (lead acetate, Pb), and probiotics (LA+LP, Pro) were administered at 10 mg / kg, 10 mg / kg, and 1×10 9 The animals were administered at a concentration of CFU / rat for 4 weeks. The specific animal experiment schedule is as follows:

[0063] [Table 1]

[0064] The body weight of each experimental group was measured every week, and the liver and kidney weight of each individual were measured after administration. During the administration period, feces were collected every week from each individual to measure the cadmium and lead concentrations in the feces, and after administration was completed, the cadmium and lead concentrations in the blood and tissues (liver and kidney) of each individual were measured. An atomic absorption spectrometer was used to measure this. In addition, after 4 weeks of administration, hematological analysis was performed to analyze the lipid and liver values ​​of each individual.

[0065] Experimental Results Example 1. Antibacterial activity experiment (MIC test) of cadmium (Cd) by probiotics and evaluation of bacterial growth (in vitro) The antibacterial activity test (MIC test) of heavy metals and bacterial growth were analyzed for each strain selected in Experimental Example 1, and the tolerance ability was evaluated. The results are shown in Table 2 below.

[0066] [Table 2]

[0067] Table 2 shows the Cd resistance of each strain. Cadmium resistance was evaluated by 19 types of antibacterial activity tests (MIC tests). The order of cadmium resistance was LA1, LGA, EF4, EFL2, SL6>LPC5>LH5>LU4, BT1>LR5, BF3>LC5>PP1(SL4)>LF2, LP3, BL3, BG7, BR3, ST3 (Table 1). The growth curve pattern of each strain also showed a similar appearance to the cadmium concentration observed in the antibacterial activity tests (MIC tests) (see Figures 1a to 1j).

[0068] Example 2. Evaluation of the heavy metal removal capacity of probiotics for cadmium (Cd) (in vitro) After reacting each strain with a 50mg / L cadmium solution for 1 hour, the residual cadmium concentration was measured. All strains were effective in removing cadmium, but LF2, SL6, LP3, LH5, LA1, and ST3 strains showed a removal rate higher than the total average value of 28.8%. The removal capacity of the six strains (LF2, SL6, LP3, LH5, LA1, and ST3) selected in the 50 mg / L cadmium solution was further evaluated in a 5 mg / L cadmium solution and a 100 mg / L cadmium solution.

[0069] The average removal capacity for a cadmium solution of 5 mg / L was 51%, with the LP3, LA1, and LH5 strains demonstrating removal capacity of over 90%. The average removal capacity for a cadmium solution of 100 mg / L was 21.6%, with the LP3, LA1, and LH5 strains demonstrating removal capacity of over 21.6% (see Figures 2 to 4).

[0070] Example 3. Antibacterial activity test (MIC test) of lead (Pb) by probiotics and evaluation results of bacterial growth (in vitro) The antibacterial activity test (MIC test) of heavy metals and bacterial growth were analyzed for each strain selected in Experimental Example 1, and the tolerance ability was evaluated. The results are shown in Table 3 below.

[0071] [Table 3]

[0072] Table 3 shows the Pb resistance of each strain, and it was found that the results showed a different pattern from the results of evaluating cadmium resistance in a total of 19 antibacterial activity experiments (MIC tests). The OD values ​​measured after 24 hours of culture in MRS or BL broth showed a similar tendency to the control group regardless of the Pb concentration (see Figures 5a to 5d).

[0073] Example 4. Evaluation of the heavy metal removal capacity of probiotics for lead (Pb) (in vitro) Each strain was reacted with a 50mg / L cadmium solution for one hour, and then the residual lead concentration was measured. Each strain was generally effective in removing lead, but it was found that the degree of effectiveness varied from strain to strain. Overall, the total average removal efficiency of the strains was about 53%, but of these, strains LF2, LP3, LR3, LA1, BL3, BR3, BT1, BG7, and EFL2 were above average, with LP3 and BL3 in particular showing high removal efficiency of about 95%.

[0074] The nine strains (LF2, LP3, LR5, LA1, BL3, BR3, BT1, BG7, EFL2) selected in the 50 mg / L cadmium solution were further evaluated for their removal capacity in 5 mg / L and 100 mg / L cadmium solutions.

[0075] The average removal rate for a 5 mg / L cadmium solution was 75.7%, with the LP3, BL3, and BR3 strains showing removal rates above average. The average removal rate for a 100 mg / L cadmium solution was 40.2%, with LP3 and LA1 showing the highest removal rates (see Figures 6 to 8).

[0076] Example 5. Evaluation of the heavy metal removal capacity of probiotics for lead (Pb) (in vitro) Morphological observation was performed using SEM (scanning electron microscope) imaging on samples administered Lactobacillus plantarum and Lactobacillus acidophilus in 100 mg / L cadmium solution and 100 mg / L lead solution.

[0077] SEM observation confirmed the precipitation and formation of cadmium or lead particles from the bright areas on the cell surface of each sample (see Figure 9).

[0078] Example 6. Evaluation of heavy metal removal capacity by probiotics through body weight and liver weight (in vivo) The effects of probiotics on body weight and liver weight were evaluated in animal experiments. The body weight and liver weight of the Cd-administered group were measured to be significantly lower than the other administration groups, but it was confirmed that the administration of probiotics (LA1+LP3) was similar to the normal experimental group. The body weight of the Pb-administered group was also measured to be significantly lower, and the administration of probiotics (LA1+LP3) was similar to the normal experimental group, but there was no significant difference in tissue weight.

[0079] [Table 4]

[0080] Example 7. Evaluation of the removal capacity of heavy metals by probiotics through excretion in feces (in vivo) The Cd concentration in feces one week after Cd administration was significantly higher in the experimental group administered probiotics (LA1+LP3). The Cd content in feces of the experimental group administered Cd alone was 65-89μg / g fecal after four weeks of administration, while it was 85.3-156.4μg / g fecal in the experimental group administered probiotics (LA1+LP3), showing a difference of about two-fold at the fourth week.

[0081] From the second week of administration, the Cd content in the feces by administration of probiotics (LA1+LP3) was approximately twice as effective as administration of Cd alone. This is because the probiotics (LA1+LP3) excreted the Cd accumulated in the body in the feces, and it can be seen that the amount of Cd accumulated in the body was reduced by the probiotics (LA1+LP3).

[0082] On the other hand, the Pb concentration in feces one week after Pb administration was significantly higher in the experimental group administered probiotics (LA1+LP3). The Pb content in feces from the experimental group administered Pb alone was 34-44μg / g fecal after four weeks of administration, while it was 48-186μg / g fecal in the experimental group administered probiotics (LA1+LP3), showing a difference of about 4.5 times at four weeks.

[0083] From the second week of administration, administration of probiotics (LA1+LP3) showed that the Pb content in the feces was approximately 2.5 times more effective at eliminating Pb than administration of Pb alone. This is because the probiotics (LA1+LP3) eliminated the Pb accumulated in the body in the feces, and it can be seen that the amount of Pb accumulated in the body was reduced by the probiotics (LA1+LP3).

[0084] In addition, it was confirmed that the excretion effect of administration of probiotics (LA1+L3P) was more effective for Pb than for Cd (see Figure 12).

[0085] [Table 5]

[0086] Example 8. Evaluation of the removal capacity of heavy metals by probiotics through content measurement in blood and tissues (liver, kidney) (in vivo) Cd and Pb administration showed significant accumulation in the blood, liver and kidneys, indicating that greater amounts had accumulated in the tissues than in the blood.

[0087] Administration of probiotics (LA1+LP3) significantly reduced the amount of heavy metals accumulated in the blood and tissues. Specifically, Cd was reduced by 68%, 56%, and 58%, and Pb was reduced by 73%, 70%, and 76%, in the blood, liver, and kidneys.

[0088] As can be seen from the results measured in feces, heavy metals adhere to the administered probiotics (LA1+LP3) and are then excreted from the body, resulting in a relative reduction in their absorption into the body (see Figure 13).

[0089] [Table 6]

[0090] Example 9. Evaluation of the heavy metal removal capacity of probiotics through measurement of hematological changes (in vivo)

[0091] [Table 7]

[0092] After 4 weeks of administration according to each experimental condition as shown in Table 7, 14 parameters were measured and analyzed. The analysis results showed that the administration of Cd or Pb caused changes in the liver and kidney conditions (toxicity) and lipid metabolism, but the administration of LA1+LP3 increased the excretion of Cd or Pb, decreasing its absorption in the body, resulting in parameter measurements relatively close to those of the normal group (control group). It was also found that the administration of LA1+LP3 alone significantly increased HDL levels.

[0093] Although the specific parts of the present invention have been described in detail above, it will be apparent to those skilled in the art that such specific techniques are merely preferred embodiments and do not limit the scope of the present invention. Therefore, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A composition for removing heavy metals, comprising one or more types of bacteria selected from the group consisting of the genera Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Streptococcus.

2. The composition for removing heavy metals according to claim 1, wherein the Lactobacillus bacteria is one or more types of bacteria selected from the group consisting of Lactobacillus acidophilus, Lactobacillus gasseri, Lactobacillus lactis, Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus helveticus reuteri, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus fermentum and Lactobacillus plantarum.

3. 2. The composition for removing heavy metals according to claim 1, wherein the Bifidobacterium bacterium is one or more types of bacteria selected from the group consisting of Bifidobacterium infantis, Bifidobacterium bifidum, Bifidobacterium lactis, Bifidobacterium longum, and Bifidobacterium breve.

4. 2. The composition for removing heavy metals according to claim 1, wherein the bacteria of the genus Enterococcus are one or more bacteria selected from the group consisting of Enterococcus faecium and Enterococcus faecalis.

5. 2. The composition for removing heavy metals according to claim 1, wherein the bacterium of the genus Pediococcus is Pediococcus pentosaceus.

6. 2. The composition for removing heavy metals according to claim 1, wherein the bacterium of the genus Streptococcus is Streptococcus thermophilus.

7. The composition for removing heavy metals according to claim 1 , wherein the composition is resistant to heavy metals.

8. 2. The composition for removing heavy metals according to claim 1, wherein the resistance has one or more properties selected from the group consisting of minimum inhibitory concentrations (MICs), bacterial growth, and heavy metal removal or binding capacity.

9. The composition for removing heavy metals according to claim 1 , wherein the heavy metal is a cationic heavy metal.

10. 10. The composition for removing heavy metals according to claim 9, wherein the cationic heavy metal is cadmium, and the bacterium has a cadmium removal ability of 22% or more in a 100 mg / L cadmium solution.

11. 10. The composition for removing heavy metals according to claim 9, wherein the cationic heavy metal is lead, and the bacterium has a lead removal ability of 22% or more in a 100 mg / L lead solution.

12. The composition has an effect of promoting excretion of cationic heavy metals, 2. The composition for removing heavy metals according to claim 1, wherein the Lactobacillus bacterium is one or more species selected from the group consisting of Lactobacillus acidophilus and Lactobacillus plantarum.

13. A pharmaceutical composition for preventing or treating heavy metal poisoning, comprising the composition of claim 1.

14. A food composition comprising the composition of claim 1.

15. A cosmetic composition comprising the composition according to claim 1.

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