Tightening and anti-wrinkle composition containing hydroxyl pinacolone 9-cis-retinoate and preparation method of firming and anti-wrinkle composition containing hydroxyl pinacolone 9-cis-retinoate

Abstract

The invention discloses a firming and anti-wrinkle composition containing hydroxyl pinacolone 9-cis retinoate and a preparation method of the firming and anti-wrinkle composition, and particularly belongs to the technical field of cosmetics. The firming and anti-wrinkle composition is prepared from the following components in percentage by mass: 0.03 to 0.3 percent of hydroxypinacolone 9-cis-retinoate, 0.01 to 0.2 percent of polydeoxyribonucleotide, 0.1 to 1 percent of fructus corni fruit extract, 2 to 5 percent of an emulsifier, 15 to 20 percent of basic grease, 0.5 to 5 percent of a humectant, 0.3 to 1 percent of a preservative, 0.1 to 1 percent of a thickening agent and the balance of deionized water, wherein the total percentage of the components is 100 percent. The firming and anti-wrinkle composition containing the hydroxypinacolone 9-cis-retinoate, the polydeoxyribonucleotide and the dogwood fruit extract, provided by the invention, has excellent firming and anti-wrinkle effects, and meanwhile, the composition is mild and non-irritant, and is suitable for being used by various people.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a firming and anti-wrinkle composition containing hydroxypinacolone 9-cis retinoic acid ester and a preparation method thereof. Background Art

[0002] As people's demand for beauty and personal care products continues to grow, the market demand for cosmetics and skin care products with firming and anti-wrinkle effects is also rising. Traditional firming and anti-wrinkle products rely on collagen supplementation, the addition of moisturizing ingredients, or some physical means to improve the firmness of the skin. However, these methods often have limited effects, and long-term use may have side effects or unstable effects.

[0003] In recent years, scientific research has found that some specific active ingredients have significant effects in improving signs of skin aging, especially reducing wrinkles and improving skin firmness. Among them, CN119139160A records that hydroxypinacolone 9-cis retinoic acid ester has become an emerging ingredient in anti-wrinkle products due to its low irritation and good stability. It can promote the renewal of skin cells and enhance the synthesis of collagen, thereby effectively improving skin sagging and wrinkles.

[0004] However, the use of a single ingredient is often difficult to achieve the desired anti-wrinkle effect, and long-term reliance on a single ingredient may also lead to reduced skin adaptability. Therefore, it is particularly important to develop a multi-ingredient complex with synergistic firming and anti-wrinkle effects.

[0005] As a biologically active substance, polydeoxyribonucleotides have been found in recent years to promote skin cell regeneration and repair damaged skin tissue. They can enhance the skin's self-repair ability and thus fight skin aging. Cornus officinalis fruit extract is rich in a variety of antioxidant ingredients that can scavenge free radicals and protect the skin from damage by the external environment. It also has a certain moisturizing and nourishing effect. Summary of the invention

[0006] In view of the deficiencies of the prior art, the object of the present invention is to provide a firming and anti-wrinkle composition containing hydroxypinacolone 9-cis retinoic acid ester, by adding hydroxypinacolone 9-cis retinoic acid ester, polydeoxyribonucleotides and Cornus officinalis fruit extract into the firming and anti-wrinkle composition to achieve a more significant firming and anti-wrinkle effect.

[0007] In order to achieve the above object, the present invention discloses the following technical solutions: In a first aspect, the present invention provides a firming and anti-wrinkle composition, which comprises the following ingredients by mass percentage: Hydroxypinacolone 9-cis retinoic acid ester 0.03-0.3%; Polydeoxyribonucleotide 0.01-0.2%; Cornus officinalis fruit extract 0.1-1%; Emulsifier 2-5%; Basic oil 15-20%; Moisturizer 0.5-5% Preservatives 0.3-1%; Thickener 0.1-1%; Make up to 100% with deionized water.

[0008] Preferably, the method for preparing the Cornus officinalis fruit extract comprises the following steps: Step 1-1. Select dried, pitted cornus officinalis fruit, crush it to obtain cornus officinalis fruit powder, and set aside; Step 1-2. Prepare an ethanol aqueous solution with a concentration of 10-20 v / v%, adjust the pH to 4-5, and mix the cornus fruit powder and the ethanol aqueous solution at a solid-liquid ratio of 1: (8-15) g / mL to obtain a mixture to be extracted; Step 1-3. Add a composite enzyme consisting of pectinase and cellulase in a mass ratio of 1: (1-3) to the mixture to be extracted, wherein the pectinase activity is 15000-50000U / g, the cellulase activity is 5000-20000U / g, and the amount of the composite enzyme added is 0.3-0.5wt% of the mass of the cornus fruit powder. After uniform mixing, first enzymolysis at 40-50°C for 0.5-1.5h, then enzymolysis at 50-60°C for 0.5-1.5h, after the gradient enzymolysis is completed, the temperature is raised to 85-95°C to inactivate the enzyme, the enzyme inactivation time is ≥20min, after the enzyme inactivation is completed, centrifuge at 40-50°C to obtain the supernatant, and then concentrate the supernatant to be alcohol-free to obtain a crude extract; Step 1-4. The crude extract obtained in step 1-3 is passed through a polyamide chromatography column, eluted with 1BV-2BV of pure water, and then eluted with 0.5BV-2.5BV of 50-80v / v% ethanol aqueous solution, wherein the elution flow rate is 0.5-1BV / h; Step 1-5. Collect the ethanol aqueous solution eluate of step 1-4, and concentrate under reduced pressure to obtain a cornus officinalis fruit extract with a relative density of 1.025-1.125.

[0009] Preferably, the emulsifier is selected from at least one of PEG-100 stearate, glyceryl stearate, and polyglyceryl-3 methyl glucose distearate.

[0010] Preferably, the base oil is selected from at least one of isononyl isononanoate, caprylic / capric triglyceride, and cetyl ethylhexanoate.

[0011] Preferably, the moisturizer is selected from at least one of 1,3-propylene glycol, butylene glycol and glycerol.

[0012] Preferably, the thickener is selected from at least one of xanthan gum, polyacrylate cross-linked polymer-6, and sodium polyacryloyldimethyl taurate.

[0013] Preferably, the preservative is selected from at least one of phenoxyethanol, p-hydroxyacetophenone and ethylhexylglycerin.

[0014] In a second aspect, the present invention provides a method for preparing the firming and anti-wrinkle composition according to the first aspect, the preparation method comprising the following steps: Step 2-1. Heat and stir the emulsifier, base oil, and hydroxypinacolone 9-cis retinoic acid ester to obtain solution A; Step 2-2. Heat and stir the thickener, preservative, moisturizer and deionized water to obtain solution B; Step 2-3. Add solution A to solution B and stir evenly to obtain a mixture C; Step 2-4: Add polydeoxyribonucleotides and cornus officinalis fruit extract to the mixture C and stir evenly to obtain the firming and anti-wrinkle composition.

[0015] Preferably, the step 2-1 is to uniformly stir the emulsifier, base oil and hydroxypinacolone 9-cis retinoic acid ester at 80° C. and 300 r / min to obtain solution A.

[0016] Preferably, the step 2-2 is to uniformly stir the thickener, preservative, moisturizer and deionized water at 80° C. and 300 r / min to obtain solution B.

[0017] Preferably, step 2-3 is to add solution A to solution B at 80° C., first homogenize at 7000 r / min for 5 min, and then stir at 500 r / min for 20 min to obtain mixture C.

[0018] Preferably, the step 2-4 is to cool the mixture C to 45° C., add polydeoxyribonucleotides and cornus officinalis fruit extract, and stir at 300 r / min for 3 min to obtain a firming and anti-wrinkle composition.

[0019] In the present invention: Hydroxypinacolone retinoic acid ester can resist fine lines and wrinkles, while promoting the production of collagen and elastin, improving the elasticity and firmness of the skin, making the skin smoother and more delicate. At the same time, it also has the effect of inhibiting tyrosinase, reducing pigmentation, improving the gloss and firmness of the skin, and making the skin tone brighter. In addition, it also has antioxidant properties, which can reduce the damage of free radicals to the skin, reduce the roughness of the skin, and can inhibit sebum secretion, making the skin more refreshed.

[0020] Polydeoxyribonucleotides can stimulate the activity of adenosine A2A receptors, affect transcription factors, and secrete growth factors such as epidermal growth factor (EGF) and fibroblast growth factor (FGF), thereby stimulating epidermal cell growth, increasing collagen synthesis, and promoting the repair and regeneration of damaged skin. At the same time, it has good anti-inflammatory properties and can reduce the inflammatory response of the skin by inhibiting the expression of inflammatory cytokines.

[0021] The cornus officinalis fruit extract provided by the present invention is rich in morroniside, has excellent antioxidant properties, can effectively remove free radicals, resist oxidative stress reactions, delay skin aging, reduce wrinkles and sagging, and at the same time, it can inhibit the activity of inflammatory factors and reduce skin redness, swelling and allergies.

[0022] Beneficial effects of the present invention: 1. The firming and anti-wrinkle composition containing hydroxypinacolone 9-cis retinoic acid ester, polydeoxyribonucleotide and cornus officinalis fruit extract provided by the present invention has excellent firming and anti-wrinkle effects. At the same time, the composition is mild and non-irritating, and is suitable for use by all kinds of people.

[0023] 2. Hydroxypinacolone 9-cis retinoic acid ester, polydeoxyribonucleotides and Cornus officinalis fruit extract play a vital role in firming and anti-wrinkle. Combined with the test results, it can be seen that these three ingredients have a certain synergistic effect in firming and anti-wrinkle. DETAILED DESCRIPTION

[0024] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0025] Unless otherwise specified, the test methods used in the embodiments and comparative examples are conventional methods; the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels; the percentages mentioned in the embodiments and comparative examples, unless otherwise specified, are mass percentages.

[0026] In the present invention: Hydroxypinacolone 9-cis retinoic acid ester: purchased from Hezhi Biotechnology (Nanjing) Co., Ltd.; Polydeoxyribonucleotides: purchased from Ruijiming (Shandong) Biotechnology Co., Ltd.; Cornus officinalis fruit: Cornaceae Cornus genus ( Cornus officinalis ) was purchased from Guangdong Zhongke Zhuoyuan Biotechnology Co., Ltd. Pectinase: purchased from Dongheng Huadao Biotechnology, enzyme activity 15000-50000U / g; Cellulase: purchased from Dongheng Huadao Biotechnology, enzyme activity 5000-20000U / g; Polyamide chromatography column: purchased from Merck, model is polyamide C66.

[0027] The remaining raw materials are commercially available.

[0028] Preparation of Cornus officinalis fruit extract Step 1-1. Select dried, pitted cornus fruit and grind it into powder in a high-speed grinder for later use; Step 1-2. Prepare an ethanol aqueous solution with a concentration of 10-20 v / v%, adjust the pH to 4-5, and mix the cornus fruit powder and the ethanol aqueous solution at a solid-liquid ratio of 1: (8-15) g / mL to obtain a mixture to be extracted; Step 1-3. Add a composite enzyme of pectinase and cellulase in a mass ratio of 1: (1-3) to the mixture to be extracted, wherein the amount of the composite enzyme added is 0.3-0.5wt% of the mass of the cornus fruit, and after uniform mixing, first keep it at 45°C for 0.5-1.5h, then keep it at 55°C for 0.5-1.5h, and after the gradient enzymatic hydrolysis is completed, heat it to 85-95°C to inactivate the enzyme, and the enzyme inactivation time is ≥20min. After the enzyme inactivation is completed, centrifuge it at 40-50°C and 10000rpm for 15min, and concentrate the supernatant obtained by centrifugation until it is alcohol-free to obtain a crude extract; Step 1-4. The crude extract obtained in step 1-3 is passed through a polyamide chromatography column, eluted with 1BV-2BV of pure water, and then eluted with 0.5BV-2.5BV of 50-80v / v% ethanol aqueous solution, wherein the elution flow rate is 0.5-1BV / h; Step 1-5. Collect the ethanol aqueous solution eluate of step 1-4, and concentrate under reduced pressure to obtain a cornus officinalis fruit extract with a relative density of 1.025-1.125.

[0029] Table 1 Preparation parameters of Cornus officinalis fruit extract

[0030] Preparation of firming and anti-wrinkle essence lotion Accurately weigh each ingredient according to the mass percentage of the raw materials in Table 2; Step 2-1. Stir the emulsifier, base oil, and hydroxypinacolone 9-cis retinoic acid ester at 80° C. and 300 r / min to obtain solution A; Step 2-2. Stir the thickener, preservative, moisturizer and deionized water at 80° C. and 300 r / min to obtain solution B; Step 2-3. At 80°C, solution A was added to solution B, homogenized at 7000 r / min for 5 min, and then stirred at 500 r / min for 20 min to obtain a mixture C; Step 2-4. After cooling the mixture C to 45° C., polydeoxyribonucleotides and cornus officinalis fruit extract were added, and the mixture was stirred at 300 r / min for 3 min to obtain a firming and anti-wrinkle essence lotion.

[0031] Table 2 Ingredients and mass percentages of essence milk

[0032] Note: “ / ” in the table means no addition.

[0033] In order to verify the role of the key ingredients in the essence milk and the relationship between the key ingredients, adjustments were made based on the formula of Example 3, as follows: Table 3 Comparison of ingredients and mass percentage of essence milk

[0034] Note: “ / ” in the table means no addition.

[0035] The preparation methods of Comparative Examples 1-5 are the same as that of Example 3, except that the missing components are not added in the corresponding steps.

[0036] Performance Testing 1 In vitro experiments Antioxidant activity test

[0037] Samples to be tested: essence lotions prepared in Examples 1-4 and Comparative Examples 1-5.

[0038] The antioxidant activity of each sample was evaluated using the DPPH (1,1-diphenyl-2-picrophenylhydrazine) free radical scavenging method. This method is based on the principle of color change after the DPPH free radical reacts with antioxidants, and the antioxidant activity is quantified by measuring the change in absorbance.

[0039] DPPH solution: Dissolve 1 mg of DPPH in 24 mL of anhydrous ethanol to prepare a 0.1 mmol / L DPPH solution, sonicate for 5 min, and shake thoroughly to mix.

[0040] Test sample group: Prepare the test samples with anhydrous ethanol to prepare a test sample solution with a concentration of 50wt%, take 2mL of the test sample solution and 2mL of DPPH solution into the same test tube, shake well, and let it stand in the dark at room temperature for 30min. Then measure the absorbance value at 520nm, which is recorded as A 待测样品 ; Control group: Simultaneously measure the absorbance value of 2 mL of DPPH solution mixed with 2 mL of anhydrous ethanol, recorded as A 对照 ; Background group: The absorbance value measured after replacing the DPPH solution in the sample group with an equal amount of anhydrous ethanol is recorded as A 10 The absorbance value measured after replacing the DPPH solution in the control group with an equal amount of anhydrous ethanol is recorded as A 20 .

[0041] The scavenging ability of different samples to be tested for DPPH free radicals was calculated according to the following formula. The results are shown in Table 4.

[0042] DPPH free radical scavenging rate = [1-(A 待测样品 -A 10 ) / (A 对照 -A 20 )]×100% Elastase inhibition assay

[0043] Samples to be tested: essence lotions prepared in Examples 1-4 and Comparative Examples 1-5.

[0044] An in vitro elastase inhibition assay was used to evaluate the effect of the samples on skin elastase activity, and a specific fluorescent substrate was used to measure the reduction in enzyme activity, thereby evaluating the anti-wrinkle and firming effects.

[0045] Perform grouping according to the kit instructions: Test sample group: Add 50 μL of the test sample solution with a concentration of 50 wt% prepared with the buffer in the kit to a black 96-well bottom plate, then add 100 μL of 0.2 U / mL elastase working solution, shake and react at room temperature for 15 minutes, then add 50 μL of 100 μg / mL elastase fluorescent conjugate to each well, incubate at room temperature in the dark for 30 minutes, and measure the fluorescence intensity at an excitation wavelength of 485 nm and an emission wavelength of 530 nm using a fluorescence microplate reader, recorded as MFI 待测样品 ; Control group: replace the sample solution with an equal amount of 50 μL buffer solution, and keep the rest unchanged. The measured fluorescence intensity is recorded as MFI 对照 ; Background group: replace the sample solution with 50 μL buffer, replace the elastase working solution with 100 μL buffer, and keep the rest unchanged. The measured fluorescence intensity is recorded as MFI 背景 .

[0046] The inhibitory ability of different samples to be tested on elastase was calculated according to the following formula. The results are shown in Table 4.

[0047] Elastase inhibition rate = [1-(MFI 待测样品 -MFI 背景 ) / (MFI 对照 -MFI 背景 ])×100% Table 4 Test results

[0048] 2 Human trials The essence milks prepared in Examples 1-4 and Comparative Examples 1-5 were used for human clinical trials.

[0049] 2.1 Security Testing

[0050] Conduct human skin occluded patch test according to the human skin patch test specified in the "Technical Specifications for Safety of Cosmetics".

[0051] 2.1.1 Test purpose: To detect the potential possibility of the test substance causing adverse reactions to human skin.

[0052] 2.1.2 Test design: The patch tester with the test substance was applied to the back skin of the subject for 24 hours. The skin was scored according to the skin reaction grading standard of the skin occlusion patch test at 30 minutes (after the indentation disappears), 24 hours and 48 hours after the removal of the test substance patch tester.

[0053] 2.1.3 Test substances: the essence lotions prepared in Examples 1-4 and Comparative Examples 1-5.

[0054] 2.1.4 Test instruments / materials: analytical balance, 1 mm spot tester, medical tape, sterile cotton swab and 1 mL syringe; 2.1.5 Test method: Volunteer requirements: Volunteers aged 18-60 who meet the test requirements will be selected as subjects and randomly divided into groups, with 30 people in each group; Test steps: The selected area does not exceed 50mm 2, a qualified spot test device with a depth of about 1mm. Put the test substance into the small chamber of the spot tester, with an amount of about 0.020g-0.025g. The control well is a blank control without adding anything. Apply the spot tester with the test substance to the back of the subject with low-sensitivity tape, and gently press it with the palm of your hand to evenly apply it on the skin for 24 hours.

[0055] Observe the skin reaction according to the standards in Table 5 30 minutes (after the indentation disappears), 24 hours and 48 hours after removing the test article patch tester, and record the observation results. The results are shown in Table 6.

[0056] Table 5 Skin reaction grading standards for occlusive patch test

[0057] Table 6 Test results of test substances

[0058] Results Analysis The test results showed that there was no positive reaction in 0 of the 30 people. The essence lotions prepared in Examples 1-4 and Comparative Examples 1-5 provided by the present invention will not cause adverse skin reactions, are very mild, and have no irritation to human skin.

[0059] 2.2 Skin elasticity test

[0060] 2.2.1 Test samples The essence lotions prepared in Examples 1-4 and Comparative Examples 1-5; Essence milk bases 1-4 are respectively based on the formula of Examples 1-4 without adding hydroxypinacolone 9-cis retinoic acid ester, polydeoxyribonucleotide and cornus officinalis fruit extract, and the missing amount is supplemented with deionized water.

[0061] 2.2.2 Test Method 130 volunteers with an average age of 30 to 45 years old with wrinkles and healthy skin without damage were selected and randomly divided into 13 groups, 10 in each group, and the above test samples were used respectively. The subjects took 2g of essence milk / essence milk base and applied it on the face, once in the morning and evening every day, for 30 consecutive days.

[0062] During the experiment, the subjects were not allowed to apply any other cosmetics on the experimental area. The skin elasticity instrument Cutometer MPA580 was used to collect data on the 0th day and the 30th day. The test area was the zygomatic region. During the measurement, the probe repeated the measurement 3 times in the same test area and took the average value.

[0063] The test parameter is R2 (the ratio of the skin's rebound value Ua when there is no negative pressure to the maximum stretch value Uf when there is negative pressure). The closer R2 is to 1, the better the skin's elasticity. The R2 change rate = R2 value on the 30th day - R2 value on the 0th day / R2 value on the 0th week.

[0064] 2.2.3 Test Results As shown in Table 7.

[0065] Table 7 Skin elasticity test results

[0066] 2.3 Analysis of test results According to the test results, the essence milk containing hydroxypinacolone 9-cis retinoic acid ester, polydeoxyribonucleotides and cornus officinalis fruit extract provided by the present invention has excellent firming and anti-wrinkle effects. At the same time, the essence milk is mild and non-irritating, and is suitable for use by all kinds of people. The effective ingredients hydroxypinacolone 9-cis retinoic acid ester, polydeoxyribonucleotides and cornus officinalis fruit extract in the essence milk play a vital role in the overall formula. At the same time, combined with the in vitro test results, it can be seen that the three ingredients of hydroxypinacolone 9-cis retinoic acid ester, polydeoxyribonucleotides and cornus officinalis fruit extract have a certain synergistic effect in firming and anti-wrinkle.

[0067] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

Claims

1. A firming and anti-wrinkle composition, characterized in that: The composition comprises the following components in percentage by mass: Hydroxypinacolone 9-cis retinoic acid ester 0.03-0.3%; Polydeoxyribonucleotide 0.01-0.2%; Cornus officinalis fruit extract 0.1-1%; Emulsifier 2-5%; Basic oil 15-20%; Moisturizer 0.5-5% Preservatives 0.3-1%; Thickener 0.1-1%; Make up to 100% with deionized water.

2. The firming and anti-wrinkle composition according to claim 1, characterized in that: The preparation method of the cornus officinalis fruit extract comprises the following steps: Step 1-1. Select dried, pitted cornus officinalis fruit, crush it to obtain cornus officinalis fruit powder, and set aside; Step 1-2. Prepare an ethanol aqueous solution with a concentration of 10-20 v / v%, adjust the pH to 4-5, and mix the cornus fruit powder and the ethanol aqueous solution at a solid-liquid ratio of 1: (8-15) g / mL to obtain a mixture to be extracted; Step 1-3. Add a composite enzyme consisting of pectinase and cellulase in a mass ratio of 1: (1-3) to the mixture to be extracted, wherein the pectinase activity is 15000-50000U / g, the cellulase activity is 5000-20000U / g, and the amount of the composite enzyme added is 0.3-0.5wt% of the mass of the cornus fruit powder. After uniform mixing, first enzymolysis at 40-50°C for 0.5-1.5h, then enzymolysis at 50-60°C for 0.5-1.5h, after the gradient enzymolysis is completed, the temperature is raised to 85-95°C to inactivate the enzyme, the enzyme inactivation time is ≥20min, after the enzyme inactivation is completed, centrifuge at 40-50°C to obtain the supernatant, and then concentrate the supernatant to be alcohol-free to obtain a crude extract; Step 1-4. The crude extract obtained in step 1-3 is passed through a polyamide chromatography column, eluted with 1BV-2BV of pure water, and then eluted with 0.5BV-2.5BV of 50-80v / v% ethanol aqueous solution, wherein the elution flow rate is 0.5-1BV / h; Step 1-5. Collect the ethanol aqueous solution eluate of step 1-4, and concentrate under reduced pressure to obtain a cornus officinalis fruit extract with a relative density of 1.025-1.

125.

3. The firming and anti-wrinkle composition according to claim 1, characterized in that: The emulsifier is selected from at least one of PEG-100 stearate, glyceryl stearate, and polyglyceryl-3 methyl glucose distearate.

4. The firming and anti-wrinkle composition according to claim 1, characterized in that: The base oil is selected from at least one of isononyl isononanoate, caprylic / capric triglyceride, and cetyl ethylhexanoate.

5. The firming and anti-wrinkle composition according to claim 1, characterized in that: The moisturizing agent is selected from at least one of 1,3-propylene glycol, butylene glycol and glycerol.

6. The firming and anti-wrinkle composition according to claim 1, characterized in that: The thickener is selected from at least one of xanthan gum, polyacrylate cross-linked polymer-6, and sodium polyacryloyl dimethyl taurate.

7. The firming and anti-wrinkle composition according to claim 1, characterized in that: The preservative is selected from at least one of phenoxyethanol, p-hydroxyacetophenone and ethylhexylglycerin.

8. The method for preparing the firming and anti-wrinkle composition according to any one of claims 1 to 7, characterized in that: The preparation method comprises the following steps: Step 2-1. Heat and stir the emulsifier, base oil, and hydroxypinacolone 9-cis retinoic acid ester to obtain solution A; Step 2-2. Heat and stir the thickener, preservative, moisturizer and deionized water to obtain solution B; Step 2-3. Add solution A to solution B and stir evenly to obtain a mixture C; Step 2-4: Add polydeoxyribonucleotides and cornus officinalis fruit extract to the mixture C and stir evenly to obtain the firming and anti-wrinkle composition.

9. The preparation method according to claim 8, characterized in that: The step 2-1 is to stir the emulsifier, base oil and hydroxypinacolone 9-cis retinoic acid ester at 80° C. and 300 r / min to obtain solution A.

10. The preparation method according to claim 8, characterized in that: The step 2-2 is to uniformly stir the thickener, preservative, moisturizer and deionized water at 80° C. and 300 r / min to obtain solution B.

11. The preparation method according to claim 8, characterized in that: The step 2-3 is to add solution A to solution B at 80° C., first homogenize at 7000 r / min for 5 min, and then stir at 500 r / min for 20 min to obtain a mixture C.

12. The preparation method according to claim 8, characterized in that: The step 2-4 is to cool the mixture C to 45° C., add polydeoxyribonucleotides and cornus officinalis fruit extract, and stir at 300 r / min for 3 minutes to obtain a firming and anti-wrinkle composition.

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