Macrocyclic CFTR modulators

Abstract

The present invention is 1 , Ar 2 , R 1 , R 2 , R 3 , R 4 and X are as described in the specification, their preparation, pharmaceutically acceptable salts thereof and their use as medicaments, pharmaceutical compositions comprising one or more compounds of formula (I), and their use as modulators of CFTR, alone or in combination with one or more therapeutically active ingredients that act as CFTR potentiators or CFTR correctors. [Formula 1] JPEG2025531206000053.jpg72170

Description

[Technical Field]

[0001] The present invention relates to novel macrocyclic compounds of formula (I) and their use as pharmaceuticals, particularly in the treatment of CFTR-related diseases and disorders, such as cystic fibrosis. The present invention also relates to related aspects, including methods for preparing such compounds, pharmaceutical compositions comprising one or more compounds of formula (I), pharmaceutical compositions comprising compounds of formula (I) in combination with one or more therapeutically active ingredients that act as CFTR modulators, where the CFTR modulators are one or more CFTR correctors (particularly type I correctors and / or type II correctors and / or type III correctors) and / or CFTR potentiators, and their use in the treatment of CFTR-related diseases and disorders. [Background technology]

[0002] Cystic fibrosis (CF; mucoviscidosis, also known as fibrocystic disease of the pancreas or pancreatic fibrosis) is a condition characterized by the cystic fibrosis transmembrane conductance regulator (Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). CF is an autosomal recessive genetic disease caused by dysfunction of the epithelial chloride / bicarbonate channel called CFTR (Fibrosis Transmembrane Conductance Regulator). CFTR dysfunction leads to dysregulated chloride, bicarbonate, and water transport at the surface of secretory epithelia, which causes viscous mucus accumulation in organs including the lungs, pancreas, liver, and intestine, resulting in multiple organ failure. Today, the most debilitating effects of CF are observed in the lungs, where—due to abnormal hydration of airway surface fluid, mucus plugging, impaired mucociliary clearance, chronic inflammation, and infection—the lungs eventually lose their function, leading to death from respiratory failure (Elborn, 2016). Human CFTR is a multidomain protein containing 1,480 amino acids. Many different mutations causing CFTR dysfunction have been found in CF patients, including loss of functional CFTR protein (class I mutations), impaired CFTR trafficking, and other mutations. These mutations result in CFTR2-related defects (Class II mutations), CFTR regulation defects (also known as gating defects; Class III mutations), CFTR conductance defects (Class IV mutations), reduced CFTR protein due to splicing defects (Class V mutations) or reduced CFTR stability (Class VI mutations), and loss of CFTR protein due to mRNA destabilization (Class VII mutations) (de Boeck, Acta Paediatr. 2020, 109(5):893-895). The CFTR2 database (http: / / cftr2.org; data as of 22.08.2022) currently contains information on 401 disease-causing mutations. By far the most frequent disease-causing mutation is a deletion of phenylalanine at position 508 (F508del; allele frequency 0.697 in the CFTR2 database), which causes misfolding of the channel during synthesis in the endoplasmic reticulum, degradation of the misfolded protein, and consequently a marked reduction in transport to the cell surface (class II mutation). The remaining F508del-CFTR that traffics to the cell surface is functional, but less functional than wild-type CFTR; i.e., F508del-CFTR also contains a gating defect (Dalemans, 1991). Approximately 40% of all CF patients are homozygous for the F508del mutation, while another ~40% are heterozygous for the F508del mutation, resulting in class II mutations. They have another disease-causing mutation in class I, II, III, IV, V, VI, or VII. Such disease-causing mutations are fairly rare, with the class III G551D mutation (allele frequency 0.0210), the class I G542X mutation (allele frequency 0.0254), and the class II N1303K mutation (allele frequency 0.0158) being the next most common.

[0003] Currently, CF is treated with a wide range of medications targeting various organs and disorders. Intestinal and pancreatic disorders are treated, once diagnosed, with the addition of pancreatic digestive enzymes to the diet. Pulmonary symptoms are primarily treated with inhaled hypertonic saline, mucolytics, anti-inflammatory agents, bronchodilators, and antibiotics (Elborn, 2016).

[0004] In addition to symptomatic treatments, CFTR modulators have been developed and approved for patients with specific CFTR mutations. These compounds either directly improve CFTR folding and CFTR trafficking to the cell surface (CFTR correctors) or improve CFTR function at the cell surface (CFTR potentiators). Other types of modulators, such as compounds that increase (mutated) CFTR mRNA levels (CFTR amplifiers) and compounds that increase the plasma membrane stability of mutated CFTR (CFTR stabilizers), such as the nucleotide-binding domain 1 (NBD1) stabilizer SION-638 (currently in Phase 1) and its follow-on molecule, NBD1-A, are still in the exploratory stage (G. Hurlbut, oral presentation at the North American Cystic Fibrosis Conference 2022). CFTR modulators can also enhance the function of non-mutated (i.e., wild-type) CFTR and are therefore being studied in disorders where increasing wild-type CFTR function may have beneficial effects, such as chronic bronchitis / COPD / bronchiectasis (Le Grand, J Med Chem. 2021, 64(11):7241-7260. Patel, Eur Respir Rev. 2020, 29(156):190068) and dry eye disease (Flores, FASEB J. 2016, 30(5):1789-1797), as well as in non-CF disorders such as acute respiratory distress syndrome (ARDS) (Erfinanda L, Sci Transl Med. 2022, 14(674):eabg8577).

[0005] CFTR modulators and combinations thereof can be discovered and optimized by assessing their ability to promote mutant CFTR trafficking and function in in vitro cultured recombinant and primary cell lines, with activity in such systems predictive of activity in CF patients.

[0006] WO2019 / 161078 discloses macrocycles as modulators of cystic fibrosis, generally 15-membered macrocycles having a (pyridine-carbonyl)-sulfamoyl moiety linked to an additional aromatic group. Further macrocycles are disclosed in WO2022 / 109573 (macrocycles having a 1,3,4-oxadiazole ring), WO2022 / 076625, WO2022 / 076626, WO2022 / 076624, WO2022 / 076621, WO2022 / 076620, WO2022 / 076618, WO2021 / 030556, and WO2021 / 030555. Macrocyclic tetrapeptides (12 or 13 members), such as the compound apicidin (CAS: 183506-66-3), have been proposed as potential agents for the treatment of CF (Hutt DM et al., ACS Med Chem Lett. 2011;2(9):703-707. doi:10.1021 / ml200136e). WO2020 / 128925 discloses macrocyclic compounds capable of modulating the activity of CFTR, the macrocyclic compounds having an optionally substituted divalent N-(pyridin-2-yl)pyridinyl-sulfonamide moiety. Other macrocyclic compounds have been shown to modulate the chloride channel CFTR. It has been described to stabilize R (Stevers LM et al., Nat Commun 2022, 13:3586). Non-macrocyclic CFTR correctors and / or potentiators of CFTR have been disclosed, for example, in WO2011 / 119984, WO2014 / 015841, WO2007 / 134279, WO2010 / 019239, WO2011 / 019413, WO2012 / 027731, WO2013 / 130669, WO2014 / 078842 and WO2018 / 227049, WO2010 / 037066, WO2011 / 127241, WO2013 / 112804, WO2014 / 071122 and WO2020 / 128768.Furthermore, specific macrocyclic compounds in which an unsubstituted phenylene group is a part of the macrocyclic compound in contrast to the 8- to 10-membered bicyclic heteroarylene of the compound of formula (I) of the present invention can be found as screening compounds (CAS registration numbers: CAS-2213100-89-9, CAS-2213100-96-8, CAS-2213100-99-1, CAS-2213101-02-9, CAS-2213101-04-1, CAS-2213101-06-3, CAS-2213101-08-5, CAS-2213101-09-6, CAS-2213101-10-7, CAS-2213101-11-1, CAS-2213101-12-1, CAS-2213101-13-2, CAS-2213101-14-3, CAS-2213101-15-4, CAS-2213101-16-5, CAS-2213101-17-6, CAS-2213101-18-7, CAS-2213101-19-8, CAS-2213101-20-1, CAS-2213101-21-1, CAS-2213101-22-1, CAS-2213101-23-1, CAS-2213101-24-1, CAS-2213101-25-2, CAS-2213101-26-3, CAS-2213101-27-4, CAS-2213101-28-5, CAS-2213101-29- 101-09-6, CAS-2213101-19-8, CAS-2213101-24-5, CAS-2215788-95-5, CAS-2215788-98-8, CAS-2215789-01-6, CAS-2215789-02-7, CAS- 2215789-09-4, CAS-2215789-15-2, CAS-2215789-20-9, CAS-2215789-24-3, CAS-2215789-35-6, CAS-2215789-37-8, CAS-2215946-94-2, CAS-2215947-04-7, CAS-2215947-13-8, CAS-2215947-24-1, CAS-2215947-34-3, CAS-2215947-44-5, CAS-2215947-51-4, CAS-2215947-6 4-9, CAS-2215947-68-3, CAS-2215947-78-5, CAS-2215947-91-2, CAS-2215954-57-5, CAS-2216342-34-4, CAS-2216342-78-6, CAS-22163 42-86-6, CAS-2216343-03-0, CAS-2216343-09-6, CAS-2216343-14-3, CAS-2216343-18-7, CAS-2216343-24-5, CAS-2216343-32-5, CAS-2 216343-38-1, CAS-2216343-45-0, CAS-2216343-53-0, CAS-2216343-59-6, CAS-2216343-64-3, CAS-2216343-74-5, CAS-2216343-76-7).

[0007] CFTR modulators can be further subdivided into CFTR correctors and CFTR potentiators. CFTR correctors improve CFTR folding and cell surface trafficking, particularly for CFTR with class II (folding and trafficking) mutations, thereby increasing cell surface expression of CFTR. CFTR potentiators increase the onset probability of cell-surface CFTR, particularly for CFTR with gating defects, including corrector-rescued class II mutants, and can thus activate CFTR additively / synergistically with CFTR correctors. Thus, potentiators and correctors are used in combination in clinical settings to treat patients with CFTR class II mutations. Many CFTR correctors have been described in literature and patents. Some of these correctors, such as VX-809 (lumacaftor), VX-661 (tezacaftor), ABBV-2222 (galicaftor), VX-445 (elexacaftor), VX-659 (bamocaftor), VX-440 (oracaftor), olacaftor), VX-121 (vanzacaftor), ABBV-C2 correctors ABBV-119 and ABBV-567, as well as PTI-801 (posenacaftor, CAS 2095064-05-2; compound of Example 2 in WO2019 / 071078), alone and / or in combination with other CFTR modulators, have been shown to be effective in treating CF patients. These potentiators have been used in clinical trials in patients with CF. Similarly, many potentiators have been described in the literature and patents. Some of these potentiators, such as VX-770 (ivacaftor), VX-561 (deutivacaftor), GLPG-1837, GLPG-2451, ABBV-3067 (navocaftor), and QBW-251 (icenticaftor), have been used alone and / or in combination with other CFTR modulators in clinical trials in patients with CF.

[0008] CFTR correctors can be further subdivided with regard to their mechanisms: it is well established that correctors that exhibit additive or synergistic behavior must have distinct, i.e., complementary, mechanisms of action, likely resulting from different binding sites on the CFTR protein. Conversely, correctors that exhibit competitive behavior are likely to share the same CFTR binding site (Okiyoneda, 2013; Veit, 2018; Veit, 2020; Fiedorczuk 2022; Marchesin 2023). Thus, the structurally related correctors VX-809 (lumacaftor), VX-661 (tezacaftor), and ABBV-2222 (galicaftor) are classified as type I correctors, corrector 4a and related compounds are classified as type II correctors, while VX-445 (elexacaftor) is classified as a type III corrector, as are the structurally related correctors VX-440 (olacaftor), VX-659 (vamocaftor), and VX-121 (banzacaftor). The ABBV-C2 corrector, ABBV-119, and ABBV-567 are likely type III correctors. PTI-801 is likely another type III corrector. Due to the additive nature of their effects, correctors with different mechanisms are often combined in clinical settings to achieve greater correction efficacy with potentiators.

[0009] VX-770 (ivacaftor, KALYDECO, N-(2,4-di-tert-butyl-5-hydroxyphenyl)-1,4-dihydro-4-oxoquinoline-3-carboxamide, CAS 873054-44-5, e.g., WO2006 / 002421, WO2011 / 072241, WO2007 / 079139, WO2007 / 134279, WO2010 / 019239, WO2013 / 130669) is a CFTR potentiator and has been reported clinically and / or in vivo. Based on in vitro assay data, VX-770 is the first CFTR modulator approved (US and EU: initial approval in 2012) for the treatment of CF patients aged 4 months and older who have a single mutation in the CFTR gene that responds to KALYDEKO. The current USPI for VX-770 (as of December 2020) lists 97 eligible CFTR mutations. VX-770 is also part of ORKAMBI (a combination of VX-809 and VX-770), SYMDEKO / SYMKEVI (a combination of VX-661 and VX-770), and TRIKAFTA / KAFTRIO (a combination of VX-661, VX-445, and VX-770), which are described below.

[0010] VX-809 (lumacaftor, 3-[6-({[1-(2,2-difluoro-1,3-benzodioxol-5-yl)cyclopropyl]carbonyl}amino)-3-methylpyridin-2-yl]benzoic acid), CAS 936727-05-8, e.g., WO2007 / 056341, WO2009 / 073757, WO2009 / 076141, WO2010 / 037066, WO 2011 / 127241), a type I CFTR corrector, was approved in the US (2014) and EU (2015) for the treatment of patients aged 2 years and older with cystic fibrosis (CF) who are homozygous for the F508del mutation in the CFTR gene as part of ORKAMBI, a combination product with the CFTR potentiator lumacaftor, ivacaftor.

[0011] VX-661 (tezacaftor, 1-(2,2-difluoro-2H-1,3-benzodioxol-5-yl)-N-{1-[(2R)-2,3-dihydroxypropyl]-6-fluoro-2-(1-hydroxy-2-methylpropan-2-yl)-1Hindol-5-yl}cyclopropane-1-carboxamide), CAS 1152311-62-0 (e.g., WO2007 / 117715, WO2011 / 119984, WO2014 / 014841) is a type I CFTR corrector approved in the United States (2018) as part of SYMDEKO and in the EU (2018) as part of SYMKEVI. SYMDEKO / SYMKEVI is a combination product of tezacaftor and the CFTR potentiator ivacaftor for the treatment of cystic fibrosis (CF) in patients 6 years of age and older who are homozygous for the F508del mutation in the CFTR gene or who have at least one mutation in the CFTR gene that responds to tezacaftor / ivacaftor based on in vitro data and / or clinical evidence. The current USPI for SYMDEKO (as of June 2022) lists 154 active CFTR mutations.

[0012] VX-445 (elexacaftor, N-(1,3-dimethyl-1H-pyrazole-4-sulfonyl)-6-[3-(3,3,3-trifluoro-2,2-dimethylpropoxy)-1H-pyrazol-1-yl]-2-[(4S)-2,2,4-trimethylpyridin-1-yl]pyridine-3-carboxamide [(4S)-2,2,4-trimethylpyrrolidin-1-yl]pyridine-3-carboxamide), CAS 2216712-66-0 (e.g., WO2016 / 057572, WO2018 / 107100, WO2019 / 152940) is a type III CFTR corrector approved in the US (2019) as part of TRIKAFTA and in the EU (2020) as part of KAFTRIO. TRIKAFTA / KAFTRIO is a combination of elexacaftor and type I CFTR corrector for the treatment of cystic fibrosis (CF) in patients aged 6 years and older who have at least one F508del mutation in the CFTR gene or a CFTR gene mutation that is responsive based on in vitro data. It is a combination product of the CFTR corrector tezacaftor and the CFTR potentiator ivacaftor. The current TRIKAFTA USPI (October 2021) lists 178 valid CFTR mutations.

[0013] ABBV-2222 (GLPG-2222, gallicaftor, 4-[(2R,4R)-4-({[1-(2,2-Difluoro-1,3-benzodioxol-5-yl)cyclopropyl]carbonyl}amino)-7-(difluoromethoxy)-3,4-dihydro-2H-chromen-2-yl]benzoic acid; CAS 1918143-53-9, e.g., WO2016 / 069757) is a Type I It is a CFTR corrector and is currently being tested in combination with the CFTR potentiator, ABBV-3067 (see below), in an open-label Phase II trial (NCT03969888) in 78 CF patients aged 18 years or older who are homozygous for the F508del-CFTR mutation.

[0014] ABBV-3067 (navocaftor, GLPG-3067, (5-(3-Amino-5-((4-(trifluoromethoxy)phenyl)sulfonyl)pyridin-2-yl)-1,3,4-oxadiazol-2-yl)methanol), CAS 2159103-66-7, e.g., WO 2017 / 208115), as mentioned above, is a CFTR potentiator currently being analyzed in combination with gallicaftor in a Phase II trial (NCT03969888).

[0015] QBW-251 (Icenticaftol, 3-Amino-6-methoxy-N-[(2S)-3,3,3-trifluoro-2-hydroxy-2-methylpropyl]-5-(trifluoromethyl)pyridine-2-carboxamide, CAS 1334546-77-8 , e.g., WO2011 / 113894), is a CFTR potentiator that has been tested in a placebo-controlled Phase I / II trial in a cohort of CF patients aged 18 years and older who carry a CFTR class III or IV CFTR mutation in one allele, i.e., a mutation that places CFTR on the cell surface and can benefit from potentiation (NCT02190604).

[0016] VX-121 (Vanzacaptor, (14S)-8-[3-(2-{dispiro[2.0.2.1]heptan-7-yl}ethoxy)-1H-pyrazol-l-yl]-12,12-dimethyl-2λ6-thia-3,9,11,18,23-pentaazatetracyclo[17.3.1.111,14.05,10]tetracoza-1(22),5,7,9,19(23),20-hexaene-2,2,4-trione [17.3.1.111,14.05,10]tetracosa-1(22),5,7,9,19(23),20-hexaene-2,2,4-trione), CAS 2374124-49-7 (e.g., WO2019 / 161078, WO2021 / 030552), is a type III CFTR corrector currently being studied in two active-controlled, double-blind, randomized phase III clinical trials in CF patients as part of a new triple combination therapy that also includes banzacaftor, the type I corrector, tezacaftor, and the CFTR potentiator, VX-561 (deuterated version of ivacaftor).

[0017] VX-561 (Dutivacaftor, N-[2-tert-butyl-4-[1,1,1,3,3,3-hexadeuterio-2-(trideuteriomethyl)propan-2-yl]-5-hydroxyphenyl]-4-oxo-1H-quinoline-3-carboxamide), CTP-656, name, CAS 1413431-07-8, for example , WO2019 / 109021, WO2019 / 018395) is a deuterated form of ivacaftor for once-daily administration.

[0018] CFTR correctors, including lumacaftor, tezacaftor, gallicaftor, Corr4a, elexacaftor, and vamocaftor, have been studied in vivo for their ability to restore folding of mutant CFTR (especially F508del-CFTR) and its trafficking to the cell surface. It has been analyzed in vitro (Van Goor, 2011; Okiyoneda, 2013; Veit, 2018; Keating, 2018; Davies, 2018). Trafficking to the cell surface can be analyzed by various assay techniques, such as anti-CFTR immunoblotting (CFTR "C band" represents transported mature CFTR), anti-CFTR cell surface ELISA, enzyme fragment complementation techniques, or CFTR-HRP fusion protein in recombinant cell lines expressing mutant CFTR proteins or primary cells from CF patients. Importantly, the combined use of collectors with different types of collector mechanisms showed additive effects on increasing cell surface expression of F508del-CFTR. This has been shown, for example, for elexacaftor + / - tezacaftor (type III corrector + / - type I corrector; Keating, 2018; Veit, 2020) or vamocaftor + / - tezacaftor (type III corrector + / - type I corrector; Davies, 2018) or type I + type II + type III corrector (Veit, 2018).

[0019] Mutant CFTR transported to the cell surface may still have impaired gating, and the addition of potentiators has been shown to significantly enhance the function of cell-surface F508del-CFTR. Various functional assays are available for analyzing CFTR function. For example, cellular expression of halide-sensitive yellow fluorescent protein can be used to measure iodide influx through functional CFTR (Galietta, 2001). Furthermore, Ussing chambers and electrophysiological measurements using recombinant epithelial cells or reconstituted bronchial epithelia from CF patients can be used to characterize the effects of CFTR modulators on CFTR function. For example, the addition of the potentiator ivacaftor to lumacaftor-corrected F508del-CFTR was shown to enhance CFTR function by nearly twofold in reconstituted bronchial epithelia from CF patients (van Goor, 2011), consistent with clinical trial data demonstrating improved FEV1 with ivacaftor addition in lumacaftor-treated patients (Boyle, 2014). Similarly, again using Ussing chamber data, we demonstrated the additivity of the type I corrector tezacaftor and the type III corrector elexacaftor in correcting F508del-CFTR function in reconstituted CF bronchial epithelium, and further demonstrated a two-fold enhancement of function with the addition of the potentiator ivacaftor. We also demonstrated an additive effect of the type III corrector elexacaftor on F508del-CFTR function compared with the combination of the type I corrector tezacaftor and the potentiator ivacaftor (Keating, 2018). This latter comparison is consistent with clinical trial data in patients homozygous for F508del-CFTR, in which the addition of elexacaftor to a baseline treatment of tezacaftor plus ivacaftor (SYMDEKO) resulted in a 10% increase in ppFEV1 (Keating, 2018).The FDA recognizes the predictive power of in vitro tests regarding the efficacy of CFTR modulators in clinical practice and has approved CFTR modulators for rare CFTR mutations based solely on in vitro evidence (Ussing chamber assays in Fisher rat thyroid epithelial cells recombinantly expressing mutant CFTR; uspi SYMDEKO, uspi KALYDEKO, uspi TRIKAFTA).

[0020] Despite the availability of a variety of CFTR modulators with complementary mechanisms for many of the common mutations, the persistence of elevated sweat chloride levels remains unclear. Patients' CFTR function has not been fully restored, nor has lung function. These findings highlight the need for novel, additional CFTR corrector mechanisms with high efficacy that could be used alone or in addition to currently available or future background therapies (i.e., SYMDEKO or ORKAMBI or TRIKAFTA or [gallicaftor + navocaftor], and any additional background therapies) that, in combination, would increase the efficacy of such CFTR modulator therapies.

[0021] It has been found that CFTR correctors with a novel mechanism (i.e., not a type I, not a type II, not a type III corrector) that have potential in the prevention and treatment of CFTR-related diseases and disorders, in particular cystic fibrosis, such as compounds of formula (I) as defined below, when used in combination with CFTR correctors with a different mechanism, such as type I correctors (lumacaftor, tezacaftor, gallicaftor) and / or type II correctors (corrector 4a) and / or type III correctors (elexacaftor, vamocaftor, oracaftor, banzacaftor; in addition, ABBV-119, ABBV-567) and / or CFTR potentiators (ivacaftor, navocaftor, isenticaftor, dutivacaftor, GLPG-1837, GLPG2451), are believed to have complementary, and even synergistic, effects in the treatment of CFTR-related diseases and disorders, in particular cystic fibrosis. Therefore, such combinations are believed to be particularly useful in the treatment of cystic fibrosis. Compounds of formula (I) can restore CFTR function in the absence and presence of potentiators, as measured by Ussing chamber assays in reconstituted tissue from CF patients harboring the F508del-CFTR mutation.

[0022] The present invention provides macrocyclic compounds that are modulators of CFTR. Accordingly, the compounds of the present invention are believed to be useful in the treatment of CFTR-related diseases and disorders, particularly cystic fibrosis. Summary of the Invention

[0023] 1) A first aspect of the present invention relates to compounds of formula (I):

[0024] [ka]

[0025] (In the formula, X is -CR X1 R X2 represents; (R X1 and RX2 together with the carbon atoms to which they are attached: -C 3-6 - cycloalkane-1,1-diyl (especially cyclopropane-1,1-diyl); -C 3-6 -cycloalkane-1,1-diyl, independently containing one C 1-3 -a or the C is substituted by alkoxy, fluoro or hydroxy; or the C is substituted by two fluoro. 3-6 -cycloalkane-1,1-diyl; -C 4-6 -heterocycloalkane-diyl having one ring nitrogen atom, and if the nitrogen has a free valence, the nitrogen is unsubstituted or substituted with one substituent, and the substituent is C 1-4 -Alkyl and -COO-C 1-3 -alkyl, 4-6 -heterocycloalkane-diyl; or -C 4-6 -heterocycloalkane-diyl having one ring oxygen atom, 4-6 -heterocycloalkane-diyl; or forming a ring in which R X1 represents hydrogen, and R X2 teeth, -C 1-6 -Alkyl (especially methyl); -C 1-4 fluoroalkyl (in particular 2,2-difluoroethyl or 2,2,2-trifluoroethyl, or additionally 2,2-difluoropropyl, 3,3,3-trifluoropropyl or 3,3-difluorobutyl); -C 3-6 -cycloalkyl; -C 1-3 -alkyl, and one -- hydroxy; -- C 1-4 -alkoxy; or -- -L X2 -ArX2 (-L X2 are independently a direct bond or C 1-3 - represents alkylene (especially methylene); Ar X2 independently represent a 5- or 6-membered heteroaryl (especially oxadiazolyl); X2 are independently unsubstituted or substituted with 1 or 2 substituents, the substituents being C 1-4 -Alkyl, C 1-3 -Alkoxy, halogen, C 3-6 -cycloalkyl and C 1-3 - independently selected from fluoroalkyl (especially trifluoromethyl); The C replaced by 1-3 - alkyl; ); and R 1 is C 1-4 - represents alkyl (especially methyl); Or a fragment

[0026] [ka]

[0027] represents a heterocycle in which R 2 is C 1-4 - represents alkyl (especially methyl); R 3 is C 1-6 - represents alkyl (especially isobutyl); R 4 represents a 5-membered heteroaryl, and the 5-membered heteroaryl is independently unsubstituted. or substituted by 1, 2 or 3 substituents, the substituents being C 1-4 -Alkyl; C 1-4 -alkoxy (especially methoxy); C 1-3 -fluoroalkyl; C 1-3 -fluoroalkoxy; halogen (especially fluoro); cyano; and C 3-6 independently selected from cycloalkyl (especially cyclopropyl); Ar 1 represents an 8- to 10-membered bicyclic heteroarylene (particularly a 10-membered bicyclic heteroarylene); the 8- to 10-membered bicyclic heteroarylene is independently unsubstituted or substituted by 1 or 2 substituents, the substituents being selected from the group consisting of C 1-4 -Alkyl, C 1-3 -fluoroalkyl, C 1-4 -Alkoxy C 1-3 independently selected from fluoroalkoxy, cyano and halogen (especially fluoro); [the above group Ar 1 In this case, the -CO- group and oxygen (i.e., Ar 1 to the rest of the molecule) is Ar 1 and Ar 2 teeth, represents phenyl or 5- to 6-membered heteroaryl (particularly pyridinyl), wherein the phenyl or 5- to 6-membered heteroaryl is unsubstituted or substituted by 1 or 2 substituents (particularly substituted by 1 or 2 substituents), and the substituents are selected from the group consisting of C 1-4 -Alkyl (especially methyl), C 1-3 -fluoroalkyl, halogen (especially fluoro), cyano, C 3-6 -cycloalkyl, C 1-6 -alkoxy (especially methoxy) and C 1-3 -fluoroalkoxy).

[0028] 2) In a second aspect, the compound is of formula (I E )

[0029] [ka]

[0030] 3) Another aspect is X is -CR X1 R X2 represents; (RX1 and R X2 together with the carbon atoms to which they are attached, C 3-6 - forms a ring which is a cycloalkane-1,1-diyl (especially a cyclopropane-1,1-diyl); or R X1 represents hydrogen, and R X2 teeth, -C 1-6 -Alkyl (especially methyl); -C 1-4 - fluoroalkyl (in particular 2,2-difluoroethyl or 2,2,2-trifluoroethyl); -C 3-6 -cycloalkyl; or - -L X2 -Ar X2 (-L X2 are independently, C 1-3 - represents alkylene (especially methylene); Ar X2 independently represent a 5-membered heteroaryl (especially oxadiazolyl); the group Ar X2 are independently unsubstituted or substituted with one substituent, said substituent being C 1-4 -Alkyl, C 1-3 -Alkoxy, halogen, C 3-6 -cycloalkyl and C 1-3 - independently selected from fluoroalkyl (especially trifluoromethyl); ); and R 1 C 1-4 - represents alkyl (especially methyl); Or a fragment

[0031] [ka]

[0032] represents a heterocycle in which It relates to compounds according to embodiment 1) or 2).

[0033] 4) Another aspect is X is -CR X1 R X2 represents; (R X1 and R X2 together with the carbon atoms to which they are attached, C 3-6 - forms a ring which is a cycloalkane-1,1-diyl (especially a cyclopropane-1,1-diyl); or R X1 represents hydrogen, and R X2 teeth, -C 1-6 -Alkyl (especially methyl); -C 1-4 -fluoroalkyl (in particular 2,2-difluoroethyl or 2,2,2-trifluoroethyl); or - -L X2 -Ar X2 (-L X2 independently represent methylene; Ar X2 independently represent a 5-membered heteroaryl (especially oxadiazolyl); the group Ar X2 are independently substituted with one substituent, said substituent being C 1-4 -Alkyl, C 1-3 -Alkoxy, halogen, C 3-6 -cycloalkyl and C 1-3 - independently selected from fluoroalkyl (especially trifluoromethyl); ); and R 1 C 1-4 - represents alkyl (especially methyl); Or a fragment

[0034] [ka]

[0035] represents a heterocycle in which It relates to compounds according to embodiment 1) or 2).

[0036] 5) Another aspect is Fragment

[0037] [ka]

[0038] It relates to compounds according to embodiment 1) or 2), wherein

[0039] 6) Another aspect is R 2 represents methyl.

[0040] 7) Another aspect is R 3 represents isobutyl.

[0041] 8) Another aspect is R 4 represents a 5-membered heteroaryl, which is independently unsubstituted or substituted by 1 or 2 substituents, and said substituents are selected from the group consisting of C 1-4 -Alkyl; C 1-4 -alkoxy (especially methoxy); C 1-3 -fluoroalkyl; halogen (especially fluoro); and C 3-6 -cycloalkyl (in particular cyclopropyl); relating to compounds according to any one of embodiments 1) to 7).

[0042] 9) Another aspect is R 4 represents a 5-membered heteroaryl, the 5-membered heteroaryl being independently substituted with 1 or 2 substituents, the substituents being C 1-4 -alkoxy (especially methoxy); halogen (especially fluoro); and C 3-6 -cycloalkyl (in particular cyclopropyl); relating to compounds according to any one of embodiments 1) to 7).

[0043] 10) Another aspect is R 4 but,

[0044] [ka]

[0045] and relates to a compound according to any one of embodiments 1) to 7).

[0046] 10a) A sub-aspect of this aspect 10) is R 4 but,

[0047] [ka]

[0048] The present invention relates to a compound according to any one of embodiments 1) to 7), which represents

[0049] 11) Another embodiment is Ar 2 but, phenyl, which is unsubstituted; or - 6-membered heteroaryl (especially pyridinyl) which is independently unsubstituted or substituted by 1 or 2 substituents (especially substituted by 1 or 2 substituents), said substituents being selected from the group consisting of C 1-4 -Alkyl (especially methyl), C 1-3 -Fluoroalkyl, halogen (especially fluoro), C 3-6 -cycloalkyl, C 1-6 -alkoxy (especially methoxy) and C 1-3 -the 6-membered heteroaryl (especially pyridinyl) independently selected from fluoroalkoxy; The present invention relates to a compound according to any one of embodiments 1) to 10), which represents

[0050] 12) Another embodiment is Ar 2 represents a 6-membered heteroaryl (particularly pyridinyl, especially pyridin-2-yl), which 6-membered heteroaryl is independently unsubstituted or substituted by 1 or 2 substituents (particularly substituted by 1 or 2 substituents), and said substituents are selected from the group consisting of C 1-4 -Alkyl (especially methyl), C 1-3 -fluoroalkyl, halogen (especially fluoro) and C 1-6-alkoxy (particularly methoxy), in accordance with embodiment 1) 10).

[0051] 13) Another embodiment is Ar 2 but:

[0052] [ka]

[0053] With respect to compounds according to any one of embodiments 1) to 10), wherein the groups A), B), C), D) and E) above each form a particular sub-embodiment, the groups A), B) and C) above form a particular sub-embodiment, and the groups D) and E) above form a particular sub-embodiment.

[0054] 13a) A further subembodiment of this embodiment 13) is Ar 2 but:

[0055] [ka]

[0056] The present invention relates to a compound according to any one of embodiments 1) to 10), wherein:

[0057] 14) Another embodiment is Ar 1 is quinolin-diyl substituted by 1 or 2 substituents, said substituents being C 1-4 -Alkyl, C 1-3 -fluoroalkyl, C 1-4 -Alkoxy C 1-3 -represents quinolin-diyl, independently selected from fluoroalkoxy, cyano, and halogen (especially fluoro); [The -CO- group and the oxygen (i.e., the group connecting the quinolin-diyl to the rest of the molecule) are intended to be attached to the quinolin-diyl in the ortho-configuration.]; relates to a compound according to any one of embodiments 1) to 13).

[0058] 15) Another embodiment is Ar 1 but,

[0059] [ka]

[0060] (The asterisk indicates that the group is attached to an oxygen (i.e., Ar 1 to the rest of the molecule).

[0061] The compounds of formula (I) have at least three stereogenic or asymmetric centers that exist in either the (R)- or (S)-configuration, as defined in each embodiment defining such compounds of formula (I). In addition, compounds of formula (I) may have one or more additional stereogenic or asymmetric centers, such as one or more additional asymmetric carbon atoms. Thus, compounds of formula (I) may exist as a mixture of stereoisomers or, preferably, as pure stereoisomers. Mixtures of stereoisomers may be separated by methods known to those skilled in the art.

[0062] Compounds of formula (I) may further include compounds having one or more double bonds, They may exist in Z- and E-configurations and / or may include compounds with substituents on the ring system, which may exist in cis- and trans-configurations relative to one another.

[0063] When a particular compound (or generic structure) is described as an (R)- or (S)-enantiomer, such description is understood to refer to the respective compound (or generic structure) in enriched, especially essentially pure, enantiomeric form. Similarly, when a particular asymmetric center of a compound is described as being in the (R)- or (S)-configuration or in a particular relative configuration, such description is understood to refer to said compound in enriched, especially essentially pure form with respect to each configuration of the asymmetric center. Similarly, a cis- or trans- description is understood to refer to each stereoisomer in enriched, especially essentially pure form with respect to each relative configuration. Similarly, when a particular compound (or generic structure) is described as a Z- or E-stereoisomer (or when a particular double bond in a compound is described as being in the Z- or E-configuration), such description is understood to refer to the respective compound (or generic structure) in enriched, especially essentially pure stereoisomeric form (or the compound in enriched, especially essentially pure form with respect to each configuration of the double bond).

[0064] The term "enriched", when used in connection with stereoisomers, is understood in the context of the present invention to mean that each stereoisomer is present relative to the total of each other stereoisomer / each other stereoisomer in a ratio of at least 70:30, in particular at least 90:10 (i.e. in a purity of at least 70% by weight, in particular at least 90% by weight).

[0065] The term "essentially pure", when used in connection with stereoisomers, is understood in the context of the present invention to mean that each stereoisomer is present in a purity of at least 95% by weight, in particular at least 99% by weight, relative to each other stereoisomer / total of each other stereoisomer.

[0066] The present invention also provides isotopically labeled, especially 2Also included are isotopically labeled compounds of formula (I) according to embodiments 1) to 16) or 17), which are identical to compounds of formula (I) except that one or more atoms have been replaced, respectively, by atoms having the same atomic number but an atomic mass different from that normally found in nature. 2 H (deuterium) labeled compounds of formula (I) and salts thereof are included within the scope of the present invention. When a substituent is specifically described as representing hydrogen, it is intended to refer to all isotopes of the atom "H", i.e., the term hydrogen used for a particular substituent includes all isotopes. 2 H (deuterium); preferably the isotope 1 H (hydrogen). Heavier isotopes of hydrogen 2 Substitution with H (deuterium) can increase metabolic stability, for example, prolonging in vivo half-life, or reducing the required dose, or reducing inhibition of cytochrome P450 enzymes, for example, improving the safety profile. In one embodiment of the present invention, the compounds of formula (I) are not isotopically labeled, or they are labeled only with one or more deuterium atoms. In a subembodiment, the compounds of formula (I) are not isotopically labeled at all. Isotopically labeled compounds of formula (I) may be prepared similarly to the methods described below, except for using appropriate isotopic species of the appropriate reagents or starting materials.

[0067] In this patent application, bonds drawn as dotted lines indicate the point of attachment of the depicted group. For example, the group

[0068] [ka]

[0069] is a 5-fluoropyridin-2-yl group.

[0070] When the plural is used for compounds, salts, pharmaceutical compositions, diseases, etc., it is intended to refer to the singular compound, salt, etc. as well.

[0071] Any reference to a compound of formula (I) according to embodiments 1) to 16) or 17) is intended to mean the compound in free base or salt form and therefore, where necessary and appropriate, also to salts (particularly pharmaceutically acceptable salts) of such compounds.

[0072] The term "pharmaceutically acceptable salt" refers to a salt that retains the desired biological activity of the subject compound and exhibits minimal undesired toxic effects. Such salts include inorganic or organic acid and / or base addition salts, depending on the presence of basic and / or acidic groups in the subject compound. References include, for example, "Handbook of Pharmaceutical Salts. Properties, Selection and Use.", P. Heinrich Stahl, Camille G. Wermuth (Eds.), Wiley-VCH, 2008; and "Pharmaceutical Salts and Co-crystals," Johan Wouters and Luc See Quere (Eds.), RSC Publishing, 2012.

[0073] The definitions set forth herein apply uniformly to compounds of formula (I) as defined in any one of embodiments 1) to 16) or 17) and apply mutatis mutandis throughout the specification and claims, unless a broader or narrower definition is given by a specific definition. It is to be understood that any definition or preferred definition of a term may independently (and together with) define and replace the respective term in any or all other terms or preferred definitions defined herein.

[0074] Whenever a substituent is described as optional, it is understood that such substituent may be absent (i.e., the respective residue is unsubstituted with respect to such optional substituent), in which case all sites with free valences (e.g., in an aromatic ring, ring carbon atoms and / or ring nitrogen atoms with free valences to which such optional substituents may be attached) are replaced with hydrogen, as the case may be. Similarly, when the term "optionally" is used with respect to (ring) heteroatoms, this term means that each optional heteroatom, etc. is absent (i.e., a group has no heteroatoms / is a carbocyclic ring / etc.) or that each optional heteroatom, etc. is present as explicitly defined.

[0075] The term "halogen" means fluorine / fluoro, chlorine / chloro or bromine / bromo; preferably fluorine / fluoro.

[0076] The term "alkyl," used alone or in combination, means a straight- or branched-chain saturated hydrocarbon group having 1 to 6 carbon atoms. x-y The term "-alkyl" (x and y are each integers) refers to a group having x to y carbon atoms. For example, C 1-6 -Alkyl groups have 1 to 6 carbon atoms. Representative examples of alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert.-butyl, 3-methyl-butyl, 2,2-dimethyl-propyl and 3,3-dimethyl-butyl. For the avoidance of any doubt, when a group is described as, for example, propyl or butyl, it is meant to be n-propyl or n-butyl, respectively. R X2 C 1-6 When referring to an alkyl group, the term is specifically used to refer to a C 1-4 - alkyl, especially methyl. 1 Ga-C 1-4 When referring to -alkyl, the term specifically refers to methyl. 1-4 -R representing alkyl 2For R, the term specifically refers to methyl. 3 Ga-C 1-6 When referring to -alkyl, the term especially refers to isobutyl.

[0077] "-C x-y The term "-alkylene-", used alone or in combination, refers to a bivalently bound alkyl group as defined above having x to y carbon atoms. Preferably, -C 1-y The points of attachment of the alkylene groups are in the 1,1-diyl, 1,2-diyl or 1,3-diyl configuration.

[0078] The term "alkoxy", used alone or in combination, means an alkyl-O- group, wherein the alkyl group is as previously defined. x-y The term "-alkoxy" (x and y are each integers) refers to an alkoxy group as defined above having x to y carbon atoms. For example, C 1-4 -alkoxy group is "C 1-4 -alkyl" has the meaning previously described; 1-4 -alkyl-O- group. Representative examples of alkoxy groups are methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, isobutoxy, sec-butoxy and tert-butoxy. Preferred is methoxy.

[0079] The term "fluoroalkyl," used alone or in combination, refers to an alkyl group, as defined above, having 1 to 3 carbon atoms, in which one or more (and in some cases all) hydrogen atoms have been replaced with fluorine. x-y The term "fluoroalkyl" (x and y are each integers) refers to a fluoroalkyl group as defined above having x to y carbon atoms. For example, C 1-3-fluoroalkyl groups have 1 to 3 carbon atoms in which 1 to 7 hydrogen atoms have been replaced by fluorine. Representative examples of fluoroalkyl groups include C1-fluoroalkyl groups, such as trifluoromethyl and difluoromethyl, as well as 2-fluoroethyl, 2,2-difluoroethyl, and 2,2,2-trifluoroethyl. R X2 C 1-4 When representing -fluoroalkyl, this term especially denotes 2,2-difluoroethyl or 2,2,2-trifluoroethyl, or additionally 2,2-difluoropropyl, 3,3,3-trifluoropropyl or 3,3-difluorobutyl.

[0080] The term "fluoroalkoxy", used alone or in combination, means an alkoxy group, as defined above, having 1 to 3 carbon atoms in which one or more (and in some cases all) hydrogen atoms have been replaced by fluorine. x-y The term "fluoroalkoxy" (x and y are each integers) refers to a fluoroalkoxy group as defined above having x to y carbon atoms. For example, C 1-3 A fluoroalkoxy group has 1 to 3 carbon atoms, and 1 to 7 hydrogen atoms are replaced by fluorine atoms. Representative examples of the fluoroalkoxy group include trifluoromethoxy, difluoromethoxy, 2-fluoroethoxy, 2,2-difluoroethoxy, and 2,2,2-trifluoroethoxy. Preferred are (C1) fluoroalkoxy groups such as trifluoromethoxy and difluoromethoxy.

[0081] The term "cycloalkyl," used alone or in combination, specifically refers to a saturated monocyclic hydrocarbon ring having 3 to 6 carbon atoms. x-y The term "-cycloalkyl" (x and y are each integers) refers to a cycloalkyl group as defined above having x to y carbon atoms. For example, C 3-6- cycloalkyl groups have 3 to 6 carbon atoms. Examples of cycloalkyl groups are cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.

[0082] "-C x-y The term "-cycloalkylene-", used alone or in combination, refers to a doubly bonded cycloalkyl group, as defined above, having x to y carbon atoms. Preferably, the points of attachment of any doubly bonded cycloalkyl group are in a 1,1-diyl configuration. Examples are cyclopropane-1,1-diyl, cyclobutane-1,1-diyl, and cyclopentane-1,1-diyl; preferably cyclopropane-1,1-diyl.

[0083] C 3-6 Examples of -cycloalkane-1,1-diyl- are cyclopropane-1,1-diyl, cyclobutane-1,1-diyl and cyclopentane-1,1-diyl, preferably cyclopropane-1,1-diyl.

[0084] The term "heterocycloalkyl," whether used alone or in combination, means, unless a broader or narrower definition is explicitly stated, a monocyclic saturated hydrocarbon ring having one or two ring heteroatoms independently selected from nitrogen, sulfur, and oxygen. x-y The term "-heterocycloalkyl" means such a heterocycle having x to y ring atoms. Examples are tetrahydrofuranyl, tetrahydropyranyl and piperidinyl. Heterocycloalkyl groups are unsubstituted or substituted as explicitly defined.

[0085] "C 4-6 -heterocycloalkane-diyl having one ring oxygen atom 4-6 The term "-heterocycloalkane-diyl" refers to a two-bonded heterocycloalkyl group having one ring oxygen atom and the remaining ring carbon atoms. 4-6-heterocycloalkane-diyl having one ring nitrogen atom 4-6 The term "-heterocycloalkane-diyl" refers to a bi-bonded heterocycloalkyl group having one ring nitrogen atom and the remaining ring carbon atoms.

[0086] The term "aryl", used alone or in combination, means phenyl or naphthyl, especially phenyl. The aryl groups mentioned above are unsubstituted or substituted as expressly defined.

[0087] For example, a heterocycle "having one or two heteroatoms independently selected from oxygen and nitrogen" or "having one oxygen atom" has exactly the indicated number and type of heteroatoms, and unless explicitly stated otherwise, the remaining ring atoms are carbon atoms.

[0088] Fragment:

[0089] [ka]

[0090] A specific example of is: quinoline-diyl, especially quinoline-5,6-diyl.

[0091] The group Ar 1 is unsubstituted or substituted as explicitly defined.

[0092] The term "heteroaryl," whether used alone or in combination, unless a broader or narrower definition is explicitly stated, refers to a 5- to 10-membered monocyclic or bicyclic aromatic ring having from 1 to 4 heteroatoms, each independently selected from oxygen, nitrogen, and sulfur. Representative examples of such heteroaryl groups include 5-membered heteroaryl groups such as furanyl, oxazolyl, isoxazolyl, oxadiazolyl, thiophenyl, thiazolyl, isothiazolyl, thiadiazolyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, and tetrazolyl; 6-membered heteroaryl groups such as pyridinyl, pyrimidinyl, pyridazinyl, and pyrazinyl; and 6-membered heteroaryl groups such as indolyl, isoindolyl, benzofuranyl, isobenzofuranyl, benzothiophenyl, indazolyl, benzimidazolyl, and benzoyl. and 8-10 membered bicyclic heteroaryl groups such as benzoxazolyl, benzisoxazolyl, benzothiazolyl, benzisothiazolyl, benzotriazolyl, benzoxadiazolyl, benzothiadiazolyl, thienopyridinyl, quinolinyl, isoquinolinyl, naphthyridinyl, cinnolinyl, quinazolinyl, quinoxalinyl, phthalazinyl, pyrrolopyridinyl, pyrazolopyridinyl, pyrazolopyrimidinyl, pyrrolopyrazinyl, imidazopyridinyl, imidazopyridazinyl, and imidazothiazolyl. The above heteroaryl groups are unsubstituted or substituted as expressly defined.

[0093] Substituent R representing "5-membered heteroaryl" 4 For the group (I), this term particularly refers to the above-mentioned 5-membered groups such as triazolyl, tetrazolyl, isoxazolyl or oxadiazolyl. In particular, this term particularly refers to 5-membered groups such as isoxazol-5-yl, 1,2,4-oxadiazol-5-yl, 2H-[1,2,3]triazol-2-yl, 2H-tetrazol-2-yl, etc. The above groups are substituted as explicitly defined.

[0094] Substituent Ar representing "5- or 6-membered heteroaryl" 2 For the substituent Ar, this term especially denotes pyridinyl, especially pyridin-2-yl.2 is unsubstituted or substituted as explicitly defined.

[0095] Substituent Ar representing 5-6 membered heteroaryl X2 In the formula (I), the heteroaryl is as defined above; it particularly represents a 5-membered heteroaryl, particularly a 5-membered heteroaryl having 1 to 3 heteroatoms selected from oxygen and nitrogen (especially oxadiazolyl). The substituent Ar representing a 5- or 6-membered heteroaryl is X2 A specific example is 3-trifluoromethyl-[1,2,4]-oxadiazol-5-yl.

[0096] The term "cyano" refers to the group --CN.

[0097] Whenever the word "between" is used to describe a range of numerical values, the endpoints of the stated range are expressly included in the range. This would apply, for example, to a temperature range of 40 When a variable is described as being between 0°C and 80°C, it is meant that the endpoints 40°C and 80°C are included in the range; or when a variable is defined as an integer between 1 and 4, it is meant that the variable is the integer 1, 2, 3, or 4.

[0098] When not used in reference to temperature, the term "about" placed before a numerical value "X" in this application means between 10% of XX and 10% of X+X, preferably between 5% of XX and 5% of X+X. In the specific case of temperatures, the term "about" placed before a temperature "Y" in this application means between temperature Y-10°C and Y+10°C, preferably between Y-5°C and Y+5°C. Furthermore, the term "room temperature" as used herein means a temperature of about 25°C.

[0099] 16) Another embodiment relates to compounds of formula (I) according to embodiment 1), which are selected from the following compounds: (3S,7S,10R,13R)-13-benzyl-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-22-((6-methoxypyridin-2-yl)methyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aS,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-22-((6-methoxypyridin-2-yl)methyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-5-fluoro-13-isobutyl-22-((6-methoxypyridin-2-yl)methyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-22-((6-methoxypyridin-2-yl)methyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (3S,7S,10R,13R)-13-benzyl-10-(2,2-difluoroethyl)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo so-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecanoic acid Hydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide;(3S,7S,10S,13R)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo -10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide;(9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazole- 5-yl)ethyl)-12-methyl-7,11,14,20-tetraoxo-22-(pyridin-2-ylmethyl)-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-12-methyl-7,11,14,20-tetraoxo-22-(pyridin-2-ylmethyl)-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-12-methyl-7,11,14,20-tetraoxo-22-(pyridin-2-ylmethyl)-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-5-fluoro-13-isobutyl-12-methyl-7,11,14,20-tetraoxo-22-(pyridin-2-ylmethyl)-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxy) (Cypridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-20-fluoro-7-isobutyl-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-13-(pyridin-2-ylmethyl)-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-N-(2-(4-fluoro-3-methoxyisoxazol-5-yl)ethyl)-7-isobutyl-6,9-dimethyl-1,5,8,11-tetraoxo-13-(pyridin-2-ylmethyl)-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9,10-trimethyl-1,5,8,11-tetraoxo-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9,10-trimethyl-1,5,8,11-tetraoxo-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3'S,7'S,13'R)-20'-fluoro-7'-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13'-((6-methoxypyridin-2-yl)methyl)-6',9'-dimethyl-1',5',8',11'-tetraoxo-2',3',4',5',6',7',8',9',11',12',13',14'-dodecahydro-1'H-spiro[cyclopropane-1,10'-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline]-3'-carboxamide; (3'S,7'S,13'R)-20'-fluoro-7'-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-13'-((6-methoxypyridin-2-yl)methyl)-6',9'-dimethyl-1',5',8',11'-tetraoxo-2',3',4',5',6',7',8',9',11',12',13',14'-dodecahydro-1'H-spiro[cyclopropane-1,10'-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline]-3'-carboxamide; (3'S,7'S,13'R)-20'-fluoro-N-(2-(4-fluoro-3-methoxyisoxazol-5-yl)ethyl)-7'-isobutyl-13'-((6-methyl) (2-thoxypyridin-2-yl)methyl)-6',9'-dimethyl-1',5',8',11'-tetraoxo-2',3',4',5',6',7',8',9',11',12',13',14'-dodecahydro-1'H-spiro[cyclopropane-1,10'-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline]-3'-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-((3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)methyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-((3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)methyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-((3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)methyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-20-fluoro-7-isobutyl-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-((3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)methyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; ((3S,7S,10R,13R)-20-fluoro-13-((5-fluoropyridin-2-yl)methyl)-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-13-((5-fluoropyridin-2-yl)methyl)-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (9S,13S,19aR,22R)-5-Fluoro-13-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-12-methyl-22-((6-methylpyridin-2-yl)methyl)-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,1 0,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-12-methyl-22-(oxazol-4-ylmethyl)-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-5-fluoro-22-((5-fluoro-6-methylpyridin-2-yl)methyl)-13-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; and (9S,13S,19aR,22R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-5-fluoro-22-((5-fluoro-6-methylpyridin-2-yl)methyl)-13-isobutyl-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide.

[0100] 17) In addition to the compounds according to embodiment 16), further compounds of formula (I) according to embodiment 1) are selected from the following compounds: (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxypyrazin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-((3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)methyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-Fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(3,3,3-trifluoropropyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (9S,13S ,19aR,22R)-22-((4,6-dimethoxypyrimidin-2-yl)methyl)-5-fluoro-13-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (3S,7S,10R,13R)-13-((4,6-dimethoxypyrimidin-2-yl)methyl)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10, 11,12,13,14-Tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-17-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; ( 3S,7S,10R,13R)-13-((4,6-dimethoxypyrimidin-2-yl)methyl)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-13-((4,6-dimethoxypyrimidin-2-yl)methyl)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-13-((4,6-dimethoxypyridin-2-yl)methyl)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-13-((4,6-dimethoxypyridin-2-yl)methyl)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxy-4-methylpyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-20-fluoro-7-isobutyl-13-((6-methoxy-4-methylpyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-N-(2-(5-cyclopropyl-2H-tetrazole) (3S,7S,10R,13R)-13-(( 4,6-Dimethoxypyridin-2-yl)methyl)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-17-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((4-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((4-methoxy-6-methylpyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-10-(3,3-difluorobutyl)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-(3-methoxybenzyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((4-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-((3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)methyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-6,9-dimethyl-13-((4-methylpyridin-2-yl)methyl)-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-10-(2,2-difluoropropyl)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-10-(2,2-difluoropropyl)-20-fluoro-7-isobutyl-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-10-(2,2-difluoropropyl)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-Fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-((4-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carbo (3S,7S,10R,13R)-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-(3-methoxybenzyl)-6,9,20-trimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]isoquinoline-3-carboxamide; and (3S,7S,10R,13R)-20-Fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-(3-methoxybenzyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide.

[0101] For the avoidance of doubt, the corresponding structures of the above compounds are shown in Tables 3 or 4 below and in case of doubt, the structures shown shall prevail.

[0102] Thus, for example, the compound of Example 28: (3S,7S,10R,13R)-20-fluoro-13-((5-fluoropyridin-2-yl)methyl)-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl) -1,2,3,4,5,6,7,8,9,10,11,12,13,14-Tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide has the structure shown in Table 3, and the compound is in the absolute configuration shown:

[0103] [ka]

[0104] Similarly, for example, the compound of Example 14: (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide has the structure shown in Table 3, and the compound is in the indicated absolute configuration:

[0105] [ka]

[0106] Similarly, for example, the compound of Example 47: (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((4-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide has the structure shown in Table 4, and the compound is in the indicated absolute configuration:

[0107] [ka]

[0108] Similarly, for example, the compound of Example 58: (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-(3-methoxybenzyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide has the structure shown in Table 4, and the compound is in the indicated absolute configuration:

[0109] [ka]

[0110] The compounds of formula (I) according to embodiments 1) to 16) or 17) and their pharmaceutically acceptable salts can be used as medicaments, for example in the form of pharmaceutical compositions for enteral (e.g., oral, in particular in the form of tablets or capsules) or parenteral (including topical application or inhalation) administration.

[0111] 18) Another embodiment relates to a pharmaceutical composition comprising a compound of formula (I) according to any one of embodiments 1) to 16) or 17) or a pharmaceutically acceptable salt thereof and at least one therapeutically inactive excipient.

[0112] Pharmaceutical compositions can be prepared by methods well known to anyone skilled in the art (see, for example, Remington, The Science and Practice of Pharmacy, 21st Edition (2005), Part 5, "Pharmaceutical Manufacturing" [published by Lippincott Williams & Wilkins]), by adding the compounds of formula (I) described above or pharmaceutically acceptable salts thereof. This can be accomplished by combining the salt to be prepared, optionally with other therapeutically useful substances, with suitable non-toxic, inert, therapeutically compatible solid or liquid carrier materials and, if necessary, conventional pharmaceutical adjuvants to form a pharmaceutical dosage form.

[0113] The present invention also relates to a method for preventing or treating a disease or disorder described herein, comprising administering to a subject a pharmaceutically effective amount of a compound of formula (I) according to embodiment 1) to 16) or 17).

[0114] The compounds of formula (I) according to embodiments 1) to 16) or 17) are useful for the treatment of CFTR-related diseases and disorders, in particular cystic fibrosis.

[0115] CFTR-related diseases and disorders may be defined to include in particular cystic fibrosis and further CFTR-related diseases and disorders selected from: - chronic bronchitis, sinusitis, constipation, pancreatitis, pancreatic insufficiency, male infertility caused by congenital bilateral absence of the vas deferens (CBAVD), mild lung disease, allergic bronchopulmonary aspergillosis (ABPA), liver disease, coagulation-fibrinolysis deficiencies such as protein C deficiency, and diabetes. - Asthma; COPD; smoking-induced COPD; and dry eye disease; and - idiopathic pancreatitis; hereditary emphysema; hereditary hemochromatosis; especially I-cell disease; lysosomal storage diseases such as pseudo-Hurler; mucopolysaccharidoses; Sandhoff / Tay-Sachs; osteogenesis imperfecta; Fabry disease; Sjogren's disease; osteoporosis; osteopenia; bone healing and growth (including bone repair, bone regeneration, decreased bone resorption, and increasing bone deposition); chloride channelopathies such as myotonia congenita (Thomson and Becker forms); Bartter's syndrome type 3; epilepsy; lysosomal storage diseases; primary ciliary dyskinesia (PCD) - a term for genetic disorders of cilia structure and / or function (Kartagener syndrome). These include PCD with situs inversus, PCD without situs inversus, and ciliary aplasia, also known as situs inversus syndrome; generalized epilepsy with febrile seizures plus (GEFS+); generalized epilepsy with febrile and afebrile seizures; myotonia; congenital paramyotonia; potassium-aggravated myotonia; hyperkalemic periodic paralysis; long QT syndrome (LQTS); LQTS / Brugada syndrome; autosomal dominant LQTS with hearing loss; autosomal recessive LQTS; LQTS with dysmorphic features; congenital and acquired LQTS; dilated cardiomyopathy; autosomal dominant LQTS; osteopetrosis; and Bartter syndrome type 3.

[0116] The term "treatment of cystic fibrosis" means any treatment for cystic fibrosis and particularly includes treatment that reduces the severity of cystic fibrosis and / or reduces the symptoms of cystic fibrosis.

[0117] The term "cystic fibrosis" refers to any form of cystic fibrosis, in particular cystic fibrosis associated with one or more genetic mutations. Preferably, such cystic fibrosis is associated with impaired CFTR trafficking (Class II mutations) or reduced CFTR stability (Class VI mutations) [in particular impaired CFTR trafficking / Class II mutations], and such impaired CFTR trafficking or reduced CFTR stability may be associated with another disease-causing mutation of the same or any other class. Such further disease-causing CFTR genetic mutations include: These include Class I mutations (loss of functional CFTR protein), (additional) Class II mutations (impaired CFTR trafficking), Class III mutations (impaired CFTR regulation), Class IV mutations (impaired CFTR conductance), Class V mutations (reduced CFTR protein due to splicing defects), and / or (additional) Class VI mutations (reduced CFTR protein due to reduced CFTR stability). The one or more genetic mutations may include, for example, F508del, A561E, and N1303K, and at least one mutation selected from I507del, R560T, R1066C, and V520F; in particular, F508del. In addition to the above, further CFTR genetic mutations include, for example, G85E, R347P, L206W, and M1101K. The genetic mutations may be heterozygous, homozygous, or compound heterozygous. In particular, the genetic mutation is heterozygous for one F508del mutation. Further CFTR genetic mutations (particularly class III and / or IV mutations) include G551D, R117H, D1152H, A455E, S549N, R347H, S945L, and R117C.

[0118] The severity of a particular cystic fibrosis / cystic fibrosis-associated genetic mutation and the effectiveness of its correction may generally be measured by examining chloride transport carried out by CFTR. For example, a patient's average sweat chloride content may be used for such an assessment.

[0119] The term "cystic fibrosis symptoms" specifically refers to elevated chloride levels in sweat; cystic fibrosis symptoms may further include chronic bronchitis; sinusitis; constipation; pancreatitis; pancreatic insufficiency; male infertility caused by congenital bilateral absence of the vas deferens (CBAVD); mild lung disease; allergic bronchopulmonary aspergillosis (ABPA); liver disease; coagulation and fibrinolysis defects such as protein C deficiency; and / or diabetes.

[0120] For the avoidance of any doubt, where a compound is described as being useful for the treatment of a disease, such compound is also suitable for use in the manufacture of a medicament for the treatment of that disease, as well as in a method for the treatment of such disease, comprising administering an effective amount of such compound to a subject in need thereof.

[0121] As used herein, the term "subject" means a mammal, particularly a human.

[0122] The present invention further relates to a method for treating cystic fibrosis, comprising administering to a subject in need thereof an effective amount of a macrocycle, particularly a 17-membered macrocycle, or a pharmaceutically acceptable salt thereof, wherein the cyclic core of the macrocycle contains one aromatic moiety (e.g., an 8- to 10-membered bicyclic heteroarylene) that is particularly attached to the remainder of the molecule / ring members of the macrocycle via: (i) a carbonyl group; and (ii) and at least one N-alkylated alpha-amino acid (particularly, the N-alkylated alpha-amino acid is attached to the carbonyl group of the beta-amino acid via its N-alkylated amino group, and particularly such an alpha-amino acid is glycine or a natural or unnatural amino acid having a hydrocarbon substituent); the macrocycle is a corrector of class II mutations of human CFTR (particularly, the folding, stability, degradation and / or trafficking of the CFTR, particularly human F508del-CFTR, are modified), and preferably the activity of the CFTR is achieved using lumacaftor. The activity / effect may be tested according to the methods disclosed in the experimental section below.

[0123] 19) Another embodiment relates to a pharmaceutical composition according to embodiment 18), wherein the composition further comprises one or more therapeutically active ingredients that act as CFTR modulators; the CFTR modulators being one or more CFTR correctors (in particular type I correctors and / or type II correctors and / or type III correctors) and / or CFTR potentiators; or pharmaceutically acceptable salts thereof.

[0124] 20) A further embodiment relates to a pharmaceutical composition according to embodiment 19), wherein the one or more therapeutically active ingredients acting as CFTR modulators are a type I corrector selected from lumacaftor, tezacaftor, and gallicaftor; and / or a type II corrector that is corrector 4a; and / or a type III corrector selected from elexacaftor, vamocaftor, oracaftor, and banzacaftor; and / or a CFTR potentiator selected from ivacaftor, navocaftor, isenticaftor, dutivacaftor, GLPG-1837, and GLPG2451; or a pharmaceutically acceptable salt thereof.

[0125] 21) A further embodiment relates to a pharmaceutical composition according to embodiment 19) or 20), wherein the one or more therapeutically active ingredients acting as CFTR modulators are a type I corrector selected from lumacaftor, tezacaftor, and gallicaftor; and / or a type II corrector that is corrector 4a; and / or a type III corrector selected from elexacaftor and banzacaftor; and / or a CFTR potentiator selected from ivacaftor, navocaftor, isenticaftor, and dutivacaftor; or a pharmaceutically acceptable salt thereof.

[0126] 22) A further embodiment relates to a pharmaceutical composition according to any one of embodiments 19) to 21), wherein the composition comprises a compound of formula (I) and one therapeutically active ingredient that acts as a CFTR modulator, and the CFTR modulator is a CFTR potentiator; or a pharmaceutically acceptable salt thereof.

[0127] 23) A further embodiment relates to a pharmaceutical composition according to any one of embodiments 19) to 22), wherein the composition comprises a compound of Formula (I) and one therapeutically active ingredient that acts as a CFTR modulator, wherein the CFTR modulator is a CFTR potentiator selected from ivacaftor, isenticaftor, and dutivacaftor; or a pharmaceutically acceptable salt thereof.

[0128] 24) A further embodiment relates to a pharmaceutical composition according to any one of embodiments 19) to 21), wherein the composition has a compound of Formula (I) and two therapeutically active ingredients that act as CFTR modulators, one of the CFTR modulators being a CFTR potentiator or a pharmaceutically acceptable salt thereof, and the second CFTR modulator being a CFTR corrector or a pharmaceutically acceptable salt thereof.

[0129] 25) A further embodiment is the composition comprising a compound of formula (I), and ivacaftor and tezacaftor, or pharmaceutically acceptable salts thereof; or - ivacaftor and lumacaftor, or pharmaceutically acceptable salts thereof; or - navocaftor and gallicaftor, or their pharmaceutically acceptable salts; The present invention relates to a pharmaceutical composition according to any one of aspects 19) to 21) or 24), having the following structure:

[0130] 26) A further embodiment is a composition comprising a compound of formula (I) and a CFTR modulator. and a third CFTR modulator is a type III corrector or a pharmaceutically acceptable salt thereof.

[0131] 27) A further embodiment is the composition comprising a compound of formula (I), and - ivacaftor, tezacaftor and elexacaftor, or pharmaceutically acceptable salts thereof; or - dutivacaftor, tezacaftor and banzacaftor, or their pharmaceutically acceptable salts; The present invention relates to a pharmaceutical composition according to any one of aspects 19) to 21) or 26), comprising:

[0132] Such combination pharmaceutical compositions according to aspects 18) to 27) are particularly useful for the prevention or treatment of a CFTR-related disease as defined herein, in particular cystic fibrosis; and in a method for the prevention or treatment of a CFTR-related disease as defined herein, in particular cystic fibrosis, comprising administering a pharmaceutically effective dose of such combination pharmaceutical composition to a subject (in particular a human) in need thereof.

[0133] Thus, the compounds of formula (I) or pharmaceutically acceptable salts thereof as defined in any one of embodiments 1) to 16) or 17) according to the present invention are intended to be used in combination (or combination therapy) with said further pharmaceutically active ingredient, as defined herein, which is a CFTR modulator (CFTR corrector and / or CFTR potentiator).

[0134] The definitions set forth herein apply uniformly to all of Embodiments 1) through 30) and apply mutatis mutandis throughout the specification and claims, unless a broader or narrower definition is given by a specific definition. It should be understood that any definition or preferred definition of a term may independently (and together with) define and replace the respective term in any or all other terms or preferred definitions defined herein.

[0135] The term "therapeutically active ingredient acting as a CFTR modulator" means a CFTR corrector (in particular a type I, type II or type III corrector) and / or a CFTR potentiator that - alone and / or in combination - has demonstrated the potential for therapeutic use (tested in in vitro and / or in vivo models, in particular in clinical trials) and / or is indicated for such therapeutic use; such therapeutic use is for CFTR-related diseases (in particular cystic fibrosis). Examples are, in particular, CFTR potentiators: ivacaftor, navocaftor, isenticaftor, dutivacaftor, GLPG-1837 and GLPG-2451; and CFTR correctors: type I correctors (lumacaftor, tezacaftor, gallicaftor), type II corrector (corrector 4a) and type III correctors (elexacaftor, vamocaftor, oracaftor, banzacaftor; and in addition ABBV-119, ABBV-567; and in addition to the above, PTI-801).

[0136] Thus, in a sub-embodiment, the present invention relates to a compound of formula (I) or a pharmaceutically acceptable salt thereof as defined in any one of embodiments 1) to 16) or 17) for use in the prevention or treatment of a CFTR-related disease as defined herein, in particular cystic fibrosis; said compound of formula (I) in combination with a CFTR potentiator or a pharmaceutically acceptable salt thereof, such as, in particular, ivacaftor, navocaftor, isenticaftor or dutivacaftor; optionally in combination with, in particular, lumacaftor, tezacaftor, gallicaftor, elexacaftor or vanzacaftor. It is intended / will be administered in further combination with a CFTR corrector such as CFTR or a pharmaceutically acceptable salt thereof.

[0137] The term "subject" means a mammal, particularly a human.

[0138] The combination treatment (or concomitant therapy) may in particular be carried out simultaneously (fixed or non-fixed dose).

[0139] "Concurrently," when referring to a dosage form, means in this application that the dosage form involved is the near-simultaneous administration of two or more active ingredients and / or treatments; by simultaneous administration, it is understood that the subject will be exposed to said two or more active ingredients and / or treatments at the same time. When administered simultaneously, the two or more active ingredients may be administered in a fixed dose combination, or in a non-fixed dose combination, where such a non-fixed dose combination may be equivalent to a fixed dose combination (e.g., by using two or more different pharmaceutical compositions to be administered at near-simultaneous times by the same route of administration), or in a non-fixed dose combination using two or more different routes or regimens of administration; in each case, the administration will result in the subject being exposed essentially simultaneously to the two or more active ingredients and / or treatments being combined. An example of the simultaneous administration of a non-fixed dose combination using two different pharmaceutical compositions to be administered at approximately the same time by the same administration route is a non-fixed dose combination in which a compound of Formula (I) as defined in any one of embodiments 1) to 16) or 17) is administered bid and each CFTR modulating agent is administered bid. Another example of the simultaneous administration of a non-fixed dose combination using two different administration routes is a non-fixed dose combination in which a compound of Formula (I) as defined in any one of embodiments 1) to 16) or 17) is administered once daily or bid and each CFTR modulating agent is administered tid. Another example of the simultaneous administration of a non-fixed dose combination using two different administration routes is a non-fixed dose combination in which a compound of Formula (I) as defined in any one of embodiments 1) to 16) or 17) is administered once daily and each CFTR modulating agent is administered bid. Another example of simultaneous administration of a non-fixed dose combination using two different routes of administration is a non-fixed dose combination in which a compound of Formula (I) as defined in any one of embodiments 1) to 16) or 17) is administered bid and each CFTR modulator is administered once daily.

[0140] "Fixed-dose combination", when referring to a dosage form, means in the present application that the relevant dosage form is the administration of one single pharmaceutical composition having two or more active ingredients, in particular the pharmaceutical composition of any one of embodiments 18) to 27), and especially the pharmaceutical composition of any one of embodiments 19) to 27).

[0141] 28) Another aspect of the invention relates to a compound of formula (I) or a pharmaceutically acceptable salt thereof as defined in any one of aspects 1) to 16) or 17), for use in the treatment of CFTR-related diseases and disorders, in particular cystic fibrosis, wherein the compound is intended to be used / intended to be administered / administered in combination with one or more therapeutically active ingredients that act as CFTR modulators; the CFTR modulators being one or more CFTR correctors (in particular type I correctors and / or type II correctors and / or type III correctors) and / or CFTR potentiators.

[0142] 29) Another embodiment relates to a compound of formula (I) or a pharmaceutically acceptable salt thereof as defined in any one of embodiments 1) to 16) or 17), for use according to embodiment 28), wherein the CFTR modulator is defined in any one of embodiments 19) to 27).

[0143] 30) Another embodiment is a CFTR modulator comprising a compound of the formula defined in any one of embodiments 1) to 16) or 17) for use according to embodiment 28) or 29), wherein the CFTR modulator is defined in embodiment 25). (I) or a pharmaceutically acceptable salt thereof.

[0144] A compound of formula (I) or a pharmaceutically acceptable salt thereof as defined in any one of embodiments 1) to 16) or 17) for use in combination in the treatment of a CFTR-related disease (particularly cystic fibrosis) as defined herein; wherein the compound of formula (I) is intended to be / is administered in combination with one or more CFTR modulators; wherein the CFTR modulators are CFTR correctors (particularly type I, type II, type III correctors) and / or CFTR potentiators or pharmaceutically acceptable salts thereof; wherein the one or more CFTR modulators are in particular as defined in any one of embodiments 19) to 27); any of the embodiments relating to a compound of formula (I) or a pharmaceutically acceptable salt thereof - a compound of formula (I) or a pharmaceutically acceptable salt thereof as defined in any one of embodiments 1) to 16) or 17) for use in combination in the treatment of a CFTR-related disease (in particular cystic fibrosis) as defined herein; wherein the compound of formula (I) is intended to be / is administered in combination with one or more CFTR modulators; a compound of formula (I) or a pharmaceutically acceptable salt thereof; - the CFTR modulator or a pharmaceutically acceptable salt thereof, which is a CFTR corrector and / or a CFTR potentiator, for use in the treatment of the CFTR-related disease (in particular cystic fibrosis); the CFTR modulator or a pharmaceutically acceptable salt thereof, which is a CFTR corrector and / or a CFTR potentiator, is (intended to be) administered in combination with the compound of formula (I); the CFTR modulator or a pharmaceutically acceptable salt thereof; - use of the compound of formula (I) or a pharmaceutically acceptable salt thereof for the manufacture of a medicament / pharmaceutical composition comprising the compound of formula (I) or a pharmaceutically acceptable salt thereof and the CFTR modulator or a pharmaceutically acceptable salt thereof, which is a CFTR corrector and / or a CFTR potentiator, for use in the treatment of the CFTR-related disease (in particular cystic fibrosis); - use of the compound of formula (I) or a pharmaceutically acceptable salt thereof for the manufacture of a medicament / pharmaceutical composition having the compound of formula (I) or a pharmaceutically acceptable salt thereof as an active ingredient for use in the treatment of a CFTR-related disease (in particular cystic fibrosis), wherein the medicament / pharmaceutical composition is (intended to be) used in combination with the CFTR modulator or a pharmaceutically acceptable salt thereof, which is a CFTR corrector and / or a CFTR potentiator; - the use of the CFTR modulator, which is a CFTR corrector and / or a CFTR potentiator, or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament / pharmaceutical composition having as an active ingredient the CFTR modulator, which is a CFTR corrector and / or a CFTR potentiator, or a pharmaceutically acceptable salt thereof, for use in the treatment of the CFTR-related disease, in particular cystic fibrosis; wherein the medicament / pharmaceutical composition is (intended to be) used (concomitantly) with the compound of formula (I) or a pharmaceutically acceptable salt thereof; - use of a pharmaceutical composition comprising the compound of formula (I) or a pharmaceutically acceptable salt thereof and the CFTR modulator or a pharmaceutically acceptable salt thereof, which is a CFTR corrector and / or a CFTR potentiator, for the treatment of the CFTR-related disease (in particular cystic fibrosis); - a medicament for use in the prevention or treatment of said CFTR-related disease (in particular cystic fibrosis); comprising said compound of formula (I) or a pharmaceutically acceptable salt thereof; and intended to be administered in combination with said CFTR modulator or a pharmaceutically acceptable salt thereof, which is a CFTR corrector and / or a CFTR potentiator; - a method for the prevention or treatment of a CFTR-related disease (particularly cystic fibrosis) comprising administering to a subject (preferably a human) in need thereof an effective amount of a compound of formula (I) or a pharmaceutically acceptable salt thereof, wherein the compound of formula (I) is a CFTR modulator or a pharmaceutically acceptable salt thereof that is a CFTR corrector and / or a CFTR potentiator. the method for prevention or treatment, wherein the compound is administered in combination with an effective amount of a salt thereof; said combination administration may be in the form of a fixed dose combination or a non-fixed dose combination; - a method for preventing or treating the CFTR-related disease (particularly cystic fibrosis), comprising administering to a subject in need thereof an effective amount of a pharmaceutical composition comprising the compound of formula (I) or a pharmaceutically acceptable salt thereof and the CFTR modulator or a pharmaceutically acceptable salt thereof, which is a CFTR corrector and / or a CFTR potentiator; and - a method for the prevention or treatment of a CFTR-related disease (in particular cystic fibrosis), comprising administering to a subject (preferably a human) in need thereof an effective amount of the CFTR modulator, which is a CFTR corrector and / or a CFTR potentiator, or a pharmaceutically acceptable salt thereof, wherein the anti-CFTR modulator, which is a CFTR corrector and / or a CFTR potentiator, is administered in combination with an effective amount of the compound of formula (I) or a pharmaceutically acceptable salt thereof; the combination may be administered as a fixed dose combination or a non-fixed dose combination; It is understood that this also relates to

[0145] Preparation of compounds of formula (I): Formula (I), Formula (I E ) can be prepared by well-known literature methods, by the methods below, by the methods described in the experimental section below, or by analogous methods. Optimum reaction conditions may vary with the specific reactants or solvents used; such conditions can be determined by one skilled in the art by routine optimization procedures. In some cases, the reaction schemes below and / or the order of carrying out the reaction steps may be modified to facilitate the reaction or to avoid unwanted reaction products. In the general reaction sequences outlined below, the generic group, R 1 , R 2 , R 3 , R 4 , Ar 1 and Ar 2 is represented by formula (I), formula (I E) are as defined for R. Other abbreviations used herein are either explicitly defined or as defined in the experimental section. In some cases, the generic group R 1 , R 2 , R 3 , R 4 , Ar 1 and Ar 2 may not be compatible with the preparation illustrated in the scheme below and would require the use of a protecting group (PG). The use of protecting groups is well known in the art (see, for example, "Protective Groups in Org. Synthesis," T.W. Greene, P.G.M. Buts, Wiley-Interscience, 1999). For this purpose, it is assumed that such protecting groups have been introduced as necessary. Optionally, the final product may be further modified, for example, by manipulating substituents to give new final products f. Such manipulations include, but are not limited to, reduction, oxidation, alkylation, acylation, hydrolysis, and transition metal-catalyzed cross-coupling reactions, which are well known to those skilled in the art. The resulting compounds may be converted into salts, in particular pharmaceutically acceptable salts, by methods known per se.

[0146] The formula (I) of the present invention E Compounds of formula (I) can be prepared according to the general reaction sequence outlined below.

[0147] Compounds of formula (I) are prepared according to one of the schemes shown below.

[0148] [ka]

[0149] Reaction Scheme A: Synthesis can be carried out using racemic or enantiomerically enriched amino acid building blocks. Appropriately protected amine building blocks A and B-acids, prepared according to procedures well described in the literature or in Reaction Schemes I and J, respectively, are treated with a peptide coupling reagent such as HATU, COMU, T3P, PyBop, or EDCI / HOBt in a solvent such as THF, DMF, or NMP in the presence of a base such as TEA or DIPEA at temperatures between −20° C. and +75° C., preferably at RT, to generate the corresponding amide intermediates AB. Deprotection of the amine function of intermediate AB is accomplished according to methods known to those skilled in the art, for example, by treatment with 4 M HCl in dioxane or preferably with TFA in the case of a Boc protecting group, or with piperidine or diethylamine in the case of an Fmoc protecting group, or by appropriate treatment in the case of other protecting groups, such as a Cbz or Alloc protecting group. The deprotected intermediate AB-amine is then reacted with an appropriately protected acid C (prepared according to procedures described in the literature or experimental section) according to the peptide coupling conditions previously described for the formation of the AB intermediate. The resulting linear intermediate ABC is then deprotected prior to the final peptide coupling macrolactamisation. Optionally, the protecting groups PG1 and PG3 are removed sequentially, but preferably they are removed simultaneously in a single step. For example, tThe Bu ester and Boc protecting groups can be removed by treatment with 4 M HCl in dioxane or, preferably, TFA, or alternatively, the allyl ester and Alloc protecting groups can be removed by palladium-catalyzed treatment, as widely reported in the literature. The linear ABC-deprotected intermediate can then be cyclized under standard conditions: this intermediate can be treated with a coupling reagent such as COMU, T3P, PyBop, EDCI / HOBt, or, preferably, HATU, in dilute conditions, such as a <0.1 M solution of the ABC starting material in a solvent such as DMF or NMP, or a mixture of solvents such as DMF / DCM (1:1), in the presence of a base such as TEA or DIPEA, at temperatures between −20°C and +75°C, preferably at RT, to yield the corresponding macrocycle cABC. Depending on the nature of the different residues, several further deprotection steps may be required to yield the final product. Final purification by preparative HPLC using standard reverse-phase or, if necessary, chiral-phase columns affords the target compounds as pure stereoisomers.

[0150] Reaction Scheme B: In a variation of Reaction Scheme A, the C moiety can be introduced stepwise, one amino acid at a time. The AB intermediate described in Reaction Scheme A and the first amino acid D-1 (either commercially available or prepared according to procedures described in the literature or in the Experimental Section below) are treated according to the peptide coupling conditions described previously to form the corresponding peptide bond. Selective deprotection of the amine functionality of ABD-1, such as removal of the Fmoc group by treatment with piperidine or diethylamine, or removal of the Cbz protecting group by hydrogenolysis over a catalyst such as Pd / C or Pd(OH)2 / C in a solvent such as EtOAc, THF, or dioxane, or preferably removal of the Boc protecting group by treatment with 4 M HCl or TFA in dioxane, provides the free amine or its ammonium salt, respectively, which can be coupled to the second amino acid D-2 in an analogous peptide coupling step. The three coupling / deprotection / coupling steps described provide the linear intermediate ABC, the same as described previously in Reaction Scheme A. The remaining steps of the synthesis to obtain the desired macrocycle cABC are the same as previously described.

[0151] [ka]

[0152] [ka]

[0153] Reaction Scheme C: In another approach, the sequence for preparing the linear intermediate ABC can be modified. The appropriately protected building block C and the amine B-amine (prepared according to procedures described in the literature or in Reaction Schemes K and J, respectively) are treated with a reagent such as HATU, COMU, T3P, PyBop, or EDCI / HOBt in the presence of a base such as TEA or DIPEA in a solvent such as THF, DMF, or NMP at temperatures between −20° C. and +75° C., preferably at RT, according to previously described peptide coupling conditions. The deprotection of the acid function of intermediate BC, i.e., removal of PG4, is carried out according to methods known to those skilled in the art, for example, by treatment with NaOH or LiOH in aqueous methanol at temperatures ranging from 0° C. to 50° C. for methyl or ethyl esters, or, preferably, by hydrogenolysis over a catalyst such as Pd / C or Pd(OH)2 / C in a solvent such as EtOAc, THF, or dioxane for benzyl esters. The deprotected intermediate BC-acid is then reacted with an appropriately protected amine building block A (prepared according to procedures described in the literature or Reaction Scheme I) following previously described peptide coupling conditions. The resulting linear ABC can then be deprotected and cyclized to produce the final product cABC as described in Reaction Scheme A.

[0154] [ka]

[0155] Reaction Scheme D: As with the transition from Reaction Scheme A to Reaction Scheme B, the C moiety in Reaction Scheme C can be introduced stepwise, one amino acid at a time. The appropriately protected acid D-1 and amine B-amine (prepared according to procedures described in the literature or experimental section or Reaction Scheme J) are treated according to the peptide coupling conditions previously described. Selective deprotection of the amine function of BD-1, i.e., PG5, such as removal of the Cbz protecting group under acidic conditions or, more preferably, removal of the Boc protecting group by treatment with 4 M HCl in dioxane or, preferably, TFA, affords the corresponding ammonium salt without removing the orthogonal protecting group PG4. The resulting intermediate amine can then be coupled to a second amino acid D-2 in an analogous peptide coupling step. The three coupling / deprotection / coupling steps described yield the same protected intermediate BC as previously described in Reaction Scheme C, at which point the remainder of the synthesis can be carried out as previously described.

[0156] [ka]

[0157] Reaction Scheme E: In another variation of Reaction Scheme C, the building block A-amine is a β- tThe aspartic acid is doubly protected on the two carboxylic acid functions with suitable orthogonal protecting groups, such as an α-benzyl ester or an α-methyl ester in the presence of a butyl ester. The corresponding linear intermediate ABC is then generated according to the sequence described in Reaction Scheme C. Double deprotection of the aspartic acid side chain and Boc amine with TFA and subsequent cyclization by previously described methods generates the cyclized intermediate cABC, still protected on A. Deprotection of the aspartic acid backbone carboxylic acid, i.e., removal of PG6, can be achieved by treatment with NaOH or LiOH in methanol / water at temperatures ranging from 0°C to 50°C for the methyl or ethyl ester, or preferably by hydrogenolysis of the benzyl ester over a catalyst such as Pd / C or Pd(OH)2 / C in a solvent such as EtOAc, THF, or dioxane. The deprotected intermediate cABC-acid is then coupled with an amine AM (commercially available or prepared according to procedures described in the literature or experimental section) according to previously described peptide coupling conditions to generate the target compound. This strategy is particularly efficient for generating libraries for probing AM moieties.

[0158] [ka]

[0159] Reaction Scheme F: The strategy described in Reaction Scheme E for stepwise introduction of the A moiety can be applied in different sequences to afford the same cABC-acid intermediate, as illustrated in Reaction Scheme F. A protected A-amine, doubly protected on the two carboxylic acid functionalities with suitable orthogonal protecting groups, such as an α-benzyl ester or an α-methyl ester in the presence of a β-allyl ester, can be coupled with the required B-acid and C building blocks in the same sequence as described in Reaction Scheme A to afford the corresponding linear intermediate ABC. Deprotection of the amine protecting group PG3 with TFA in the case of a Boc protecting group, followed by removal of the aspartic acid side chain protecting group PG1 by treatment with 1,3-dimethylbarbituric acid and Pd(PPh3)4 in a solvent such as DCM in the case of an allyl protecting group, leaves only the previously described cyclization to afford intermediate cABC, which is still protected on the A moiety as described in Reaction Scheme E. The remaining steps of the synthesis to afford the desired macrocycle cABC are the same as previously described.

[0160] [ka]

[0161] Reaction Scheme G: In a variation of Reaction Scheme F, very similar to Reaction Schemes B and D, moiety C can be introduced stepwise, one amino acid at a time. Furthermore, amino acid D-1 itself can be prepared stepwise by introducing the desired side chain R1 into the already assembled ABD1 precursor. The amine-deprotected AB intermediate previously described in Reaction Scheme F can be coupled with an unsubstituted amino acid precursor of D-1, such as an NH-Boc or, preferably, an NH-nosyl-amino acid, according to the peptide coupling conditions previously described. The NH-nosyl functionality can then be alkylated by treatment with the desired alkyl halide, such as a bromide or, preferably, an iodide, in the presence of a base such as KCO, or, preferably, via a Mitsunobu reaction with the desired alcohol, performed according to standard conditions well known to those skilled in the art, for example, by treatment with DEAD or DIAD together with a phosphine ligand, such as triphenylphosphine, in a solvent such as THF or dioxane at temperatures ranging from -80°C to 60°C. The nosyl activation / protecting group can then be removed by standard treatment with thiophenol in the presence of a base such as K2CO3 in a solvent such as DMF to give the corresponding deprotected intermediate. Amino acid D-2 can be coupled to this intermediate according to the conditions illustrated in Reaction Scheme B. The three coupling / deprotection / coupling steps described allow for the formation of the corresponding hydroxyl group as previously described in Reaction Scheme F. The same deprotected linear intermediate ABC as above is obtained. The remaining steps of the synthesis to obtain the desired macrocycle cABC are the same as previously described.

[0162] [ka]

[0163] Reaction Scheme H: In a further modification of Reaction Scheme F, the α-carboxylic acid protecting group of the A-amine building block can be solid-phase, such as a polymer-bound support, allowing for stepwise solid-phase peptide synthesis of the cyclized macrocycle precursor according to established methods well known to those skilled in the art of polymer-supported peptide synthesis. For example, an amino acid, A-acid, appropriately orthogonally protected on the amine function, e.g., by an Fmoc protecting group, and on the β-carboxylic acid function, e.g., by an allyl ester, can be introduced onto a Wang resin by treatment with coupling reagents such as HOBt and DMAP, and DCC or DIC, in a solvent mixture such as DCM / DMF that allows for appropriate swelling of the polymer beads. Deprotection of the Fmoc protecting group, followed by a subsequent sequence of peptide couplings using standard conditions for polymer peptide synthesis, allows for the stepwise introduction of different building blocks, B-acid, D1, and finally an appropriately protected D2, e.g., alloc-protected D2, to give a polymer-supported linear peptide ABC similar to that described in Reaction Scheme F. Double deprotection of the allyl ester and N-alloc protecting groups can be achieved by treatment with a palladium catalyst, optionally in the presence of 1,3-dimethylbarbituric acid, to give the still-supported linear peptide. In these circumstances, cyclization under standard peptide coupling conditions can be carried out without risk of oligomer formation. The macrocycle cABC-acid described in Reaction Scheme F can then be cyclized using, for example, a mixture of TFA / HO (95 / 5) in an acidic atmosphere. The cABC-acid can then be coupled with the appropriate AM amine using the coupling conditions described above to give the desired compound.

[0164] [ka]

[0165] Building block A may be commercially available, prepared as described in the literature, or prepared as illustrated in Reaction Scheme I. t A suitably orthogonally protected A-acid, such as the butyl ester or β-allyl ester of N-Boc aspartic acid, is coupled to the desired AM amine according to standard peptide coupling conditions by treatment with COMU or T3P, HATU, PyBop, or another peptide coupling reagent in the presence of a base such as TEA or DIPEA in a solvent such as THF, DMF, or NMP at temperatures between −20° C. and +75° C., preferably at RT. The resulting intermediate can then be selectively deprotected on the amine function without removing the β-ester protecting group PG1 under standard conditions well established in the art of protecting group chemistry. Specifically, the β- t Treatment with piperidine or diethylamine to remove N-Fmoc in the presence of a butyl ester, or with TFA or 4M HCl in dioxane to remove N-Boc in the presence of a β-allyl ester, makes it possible to obtain the desired building block A as its free base or its ammonium salt, respectively.

[0166] [ka]

[0167] Building block B, B-acid or B-amine may be prepared as described in the literature or as illustrated in Reaction Scheme J. Suitable salicylic acid derivatives protected on the carboxylic acid function as esters such as methyl, ethyl or benzyl esters are commercially available, or may be prepared as described in the literature or as described in the experimental section. Similarly, amino alcohols protected on the amine function with a Boc or Cbz group are commercially available. These amino alcohols are commercially available, readily prepared from the corresponding amino acids as described in the literature, or may be prepared as described in the experimental section. The alcohol function of this amino alcohol can be activated by treatment with methanesulfonyl chloride or toluenesulfonyl chloride or a similar activating agent in the presence of a base such as DIPEA or TEA, and reacted with the phenol function of a salicylic acid ester derivative in a solvent such as THF or DMF to give the doubly protected B building block. Alternatively, these two building blocks can be reacted together according to the Mitsunobu method by treatment with a phosphine ligand such as triphenylphosphine and a DEAD or DIAD reagent in a solvent such as THF or dioxane at temperatures ranging from −20° C. to 60° C. The resulting orthogonally protected intermediate can then be selectively deprotected on the acid or amine functions to give the corresponding building blocks, B-acid or B-amine, respectively. For example, saponification of the methyl ester with aqueous NaOH or LiOH or hydrogenolysis of the benzyl ester over a palladium catalyst such as charcoal supported Pd or Pd(OH)2 can provide the corresponding B-acid. Alternatively, Boc deprotection by treatment with TFA or hydrogenolysis of the Cbz protected amine in the case of the methyl ester provides the corresponding B-amine.

[0168] [ka]

[0169] Building block C may be prepared from the key intermediate D-1 amine as illustrated in Reaction Scheme K. Intermediate D-1 may be commercially available, prepared as described in the literature, or prepared as illustrated in this scheme. A suitable PG8-protected bromoacetate ester derivative (e.g., methyl, ethyl, or benzyl ester) can be reacted with the appropriate amine R1NH2 in a solvent such as MeCN, acetone, or DMF in the presence of a base such as K2CO3 or DIPEA at temperatures ranging from RT to 80°C to generate the amine D-1. Alternatively, a suitable PG8-protected amino acid ester derivative (e.g., methyl, ethyl, or benzyl ester) can be reacted with nitrosulfonylbenzene chloride in a solvent such as DCM or THF in the presence of a catalytic amount of DMAP to give the corresponding N-(N ... The resulting amine is a -nosyl-protected amine. Alkylation of the sulfonamide nitrogen can then be achieved by the Mitsunobu method, as previously described, using a phosphine ligand such as triphenylphosphine and a DEAD or DIAD reagent in the presence of the desired alcohol R1OH at temperatures ranging from 0°C to 80°C in a solvent such as THF or dioxane. Subsequent cleavage of the nosyl group can be achieved by treatment with thiophenol in the presence of a base such as K2CO3 in a solvent such as DMF or DCM to give the amine building block D-1. Coupling with commercially available or literature-prepared D-2 amino acids can then be achieved using standard peptide coupling methods as described above. Deprotection of the ester can then be achieved by treatment with aqueous NaOH or LiOH in the case of methyl or ethyl esters, or by hydrogenolysis of the benzyl ester over a palladium catalyst such as Pd on charcoal or Pd(OH)2 to give the desired C building block.

[0170] The following examples are provided for the purpose of illustrating the present invention. These examples are for illustrative purposes only and are not to be construed as limiting the invention in any manner.

[0171] Experimental section I. Chemistry All temperatures are given in °C. Commercially available starting materials were used as received without further purification. Unless otherwise noted, all reactions were carried out in oven-dried glassware under a nitrogen atmosphere. Compounds were purified by flash column chromatography on silica gel or by preparative HPLC. Compounds described in this invention were identified based on LC-MS data (retention time t R are given in min; molecular weights obtained from mass spectrometry are given in g / mol.) using the conditions described below. When the compounds of the invention appear as a mixture of conformational isomers, especially when visible in their LC-MS spectra, the retention time of the most abundant isomer is given.

[0172] Analytical LC-MS equipment: HPLC pump: Binary gradient pump, Agilent G4220A or equivalent Autosampler: Gilson (with Gilson 845z injector) LH215 or equivalent Column compartment: Dionex TCC-3000RS or equivalent Gas removal machine: Dionex SRD-3200 or equivalent Make-up pump: Dionex HPG-3200SD or equivalent DAD detector: Agilent G4212A or equivalent MS detector: Single quadrupole mass spectrometer, Thermo Finnigan MSQPlus or equivalent ELS detector: Sedere SEDEX 90 or equivalent

[0173] LC-MS under acidic conditions Method A: Column: Zorbax SB-aq (3.5 μm, 4.6 x 50 mm). Conditions: MeCN [eluent A]; water + 0.04% TFA [eluent B]. Gradient: 95% B to 5% B in 1.5 min (flow rate: 4.5 mL / min). Detection: UV / Vis + MS.

[0174] Method B: Column: Zorbax RRHD SB-aq (1.8 μm, 2.1 x 50 mm). Conditions: MeCN [eluent A]; water + 0.04% TFA [eluent B]. Gradient: 95% B to 5% B in 2.0 min (flow rate: 0.8 mL / min). Detection: UV / Vis + MS.

[0175] Method C: Column: Waters XBridge C18 (5 μm, 4.6 x 30 mm) Conditions: MeCN [eluent A]; water + 0.04% TFA [eluent B]. Gradient: 95% B to 5% B in 1.5 min (flow rate: 4.5 mL / min). Detection: UV / Vis + MS.

[0176] Method D: Column: Waters BEH C18 (2.1 x 50 mm, 2.5 μm). Conditions: MeCN [eluent A]; water + 0.04% TFA [eluent B]. Gradient: 95% B to 5% B in 2.0 min (flow rate: 0.8 mL / min). Detection: UV / Vis + MS.

[0177] Method E: Column: Waters XBridge C18 (2.5 μm, 4.6 × 30 mm). Conditions: MeCN [eluent A]; water + 0.04% TFA [eluent B]. Gradient: 95% B to 5% B in 1.5 min (flow rate: 4.5 mL / min). Detection: UV / Vis + MS.

[0178] Method F: Column: Waters XSelect CSH C18 (3.5 μm, 2.1 x 30 mm). Conditions: MeCN + 0.1% formic acid [eluent A]; water + 0.1% formic acid [eluent B]. Gradient: 95% B to 2% B in 1.6 min (flow rate 1 mL / min). Detection: UV / Vis + MS.

[0179] Method G: Column: Waters Atlantis T3 (3.0 μm, 2.1 x 50 mm). Conditions: MeCN + 0.1% formic acid [eluent A]; water + 0.1% formic acid [eluent B]. Gradient: 95% B to 2% B in 5 min (flow rate 0.8 mL / min). Detection: UV / Vis + MS.

[0180] Method H: Waters Acquity Binary, Solvent Manager; MS: Waters SQ Detector or Xevo TQD or SYNAPT G2 MS; DAD: Acquity UPLC PDA Detector; ELSD: Acquity UPLC ELSD. Column: Waters ACQUITY UPLC CSH C18 1.7 μm 2.1 x 50 mm, thermostated at 60 °C in an Acquity UPLC Column Manager. Eluents: A: H2O + 0.05% formic acid; B: MeCN + 0.045% formic acid. Method: Gradient: 2% B to 98% B in 2.0 min. Flow rate: 1.0 mL / min. Detection: UV 214 nm and ELSD and MS. R is written in min.

[0181] LC-MS under basic conditions Method I: Column: Waters BEH C18 (2.5 μm, 2.1 × 50 mm). Conditions: Water / NH3 [c(NH3) = 13 mmol / L] [Eluent A]; MeCN [Eluent B]. Gradient: 5% B to 95% B in 2 min (flow rate 0.8 mL / min). Detection: UV / Vis + MS.

[0182] Method J: Column: Waters XSelect CSH C18 (3.5 μm, 2.1 × 30 mm). Conditions: 95% MeCN + 5% water / NH4HCO3 [c(NH4HCO3) = 10 mmol / L] [eluent A]; water / NH4HCO3 [c(NH4HCO3) = 10 mmol / L] [eluent B]. Gradient: 95% B to 2% B in 1.6 min (flow rate 1 mL / min). Detection: UV / Vis + MS.

[0183] GC-MS Agilent 6890N / Column: RXi-5MS 20m, ID 180μm, df 0.18μm; velocity 50cm / s, He carrier gas; 100°C to 250°C in 4.5min; Detection: MS.

[0184] Preparative HPLC equipment: Gilson LH215, Gilson 333 / 334 HPLC pump with Dionex SRD-3200 degasser, Dionex ISO-3100A Make-up Pump, Dionex DAD-3000 DAD Detector, Single Quadrupole Mass Spectrometer MS Detector, Thermo Finnigan MSQ Plus, MRA100-000 Flow Splitter, Polymer Laboratories PL-ELS1000 ELS Detector

[0185] Preparative HPLC under basic conditions Column: Waters XBridge (10 μm, 75 × 30 mm). Conditions: MeCN [eluent A]; water + 0.5% NH4OH (25% aqueous solution) [eluent B]; gradient: see Table 1 (flow rate: 75 mL / min). The percentage of eluent A at the start (x) is determined depending on the polarity of the compound to be purified. Detection: UV / Vis + MS.

[0186] [Table 1]

[0187] Preparative HPLC under acidic conditions Column: Waters Atlantis T3 (10 μm, 75 × 30 mm). Conditions: MeCN [eluent A]; water + 0.5% HCOH [eluent B]; gradient: see Table 2 (flow rate: 75 mL / min). The percentage of eluent A at the start (x) is determined depending on the polarity of the compound to be purified. Detection: UV / Vis + MS.

[0188] [Table 2]

[0189] Preparative HPLC for chiral separations In most cases, the desired diastereomer can be isolated or purified by standard preparative-scale HPLC according to standard methods well known to those skilled in the art. In some cases, the use of chiral chromatography columns is recommended to separate complex mixtures of diastereomers. Best results are obtained when using chiral stationary phase columns, such as Chiralpak IA, IB, or IC columns based on immobilized amylose or cellulose chiral phases, with an isocratic elution based on mixtures of MeCN with EtOH or MeOH in ratios varying from 9:1 to 1:9. To compensate for the presence of ionizable functional groups in the compounds to be purified, modifiers such as 0.1% diethylamine for basic derivatives or 0.1% formic acid for acidic derivatives can be added to the solvent mixture. In some cases, supercritical fluid chromatography was used using the same chiral stationary phase columns described above with an isocratic elution consisting of 50% to 90% supercritical carbon dioxide with EtOH, MeOH, or a 1:1 EtOH:MeCN mixture. Detection: UV / Vis.

[0190] Abbreviations (used above and below): AcOH acetic acid Ac2O acetic anhydride Alloc allyloxycarbonyl anh. anhydrous aq. aqueous solution ATM atmosphere BnBr benzyl bromide Boc tert-butoxycarbonyl Boc2O Di-tert-butyl dicarbonate BOP (benzotriazol-1-yloxy)-tris(dimethylamino)-phosphonium hexafluorophosphate BuLi n-butyllithium CDI 1,1'-carbonyldiimidazole CD3I iodomethane-d3 CHCl3Chloroform COMU (1-cyano-2-ethoxy-2-oxoethylideneaminooxy)dimethylamino-morpholino-carbenium hexafluorophosphate Cu(OAc)2Copper(II) acetate d day DBU 1,8-diazabicyclo[5.4.0]undec-7-ene DCC N,N'-dicyclohexylcarbodiimide DCE 1,2-dichloroethane DCM dichloromethane DEAD Diethyl azodicarboxylate DIAD Diisopropyl azodicarboxylate DIBAL / DIBAL-H Diisobutylaluminum hydride DIC N,N'-diisopropylcarbodiimide DIPEA Diisopropyl-ethylamine, Huenig's base DMAP 4-dimethylaminopyridine DMF Dimethylformamide DMSO dimethyl sulfoxide DPPA Diphenylphosphoryl azide dppf 1,1'-bis(diphenylphosphino)ferrocene EDC 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Et Ethyl Et2O diethyl ether EtOAc ethyl acetate EtOH ethanol Evaporated Evaporated under vacuum Example: Example FC Silica gel flash chromatography FDPP Pentafluorophenyl diphenylphosphinate Fmoc 9-Fluorenylmethoxycarbonyl h time HATU (1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate Hept Heptane Hex HOBT 1-Hydroxybenzotriazole HPLC High Performance Liquid Chromatography HV high vacuum conditions i Bu isobutyl i Pr isopropyl i PrMgCl Isopropyl magnesium chloride iPrOH Isopropyl Alcohol i PrOAc Isopropyl acetate KOAc Potassium Acetate KO t Bu potassium tert-butoxide LAH Lithium aluminum hydride LC-MS Liquid Chromatography-Mass Spectrometry Lit. Literature M mol / l mCPBA m-chloroperbenzoic acid Me methyl MeCN acetonitrile MeI iodomethane Meldrum's Acid 2,2-Dimethyl-1,3-dioxane-4,6-dione MeOH Methanol mL milliliter min mix. mixture MOM methoxymethyl MW Microwave NaBH(OAc)3 Sodium triacetoxyborohydride NCS N-chlorosuccinimide NMP N-methyl-2-pyrrolidone Nosyl 4-nitrobenzenesulfonyl n Pr n-propyl OAc acetate org.organic Pd( tBu3P)2 Bis(tri-tert-butylphosphine)palladium(0) Pd(OAc)2 Palladium(II) Acetate Pd / C Palladium on activated carbon Pd(OH)2 / C Palladium hydroxide on activated carbon (Pearlman catalyst) Pd2(dba)3 tris(dibenzylideneacetone)dipalladium(0) PdCl2(PPh3)2 Bis(triphenylphosphine)palladium(II) dichloride Pd(dppf)Cl2 [1,1'-bis(diphenylphosphino)ferrocene]dichloropalladium(II) Pd(dppf)Cl2·DCM [1,1'-Bis(diphenylphosphino)-ferrocene]dichloropalladium(II) dichloromethane complex Pd(PPh3)4 Tetrakis(triphenylphosphine)palladium(0)Ph Phenyl PhMe Toluene PPh3 Triphenylphosphine prep. for preparative preparation PTFE Polytetrafluoroethylene PyBOP (benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate PyClop chlorotripyrrolidinophosphonium hexafluorophosphate rac racemic RM reaction mixture Rochelle salt Potassium sodium tartrate RT room temperature RuPhos 2-Dicyclohexylphosphino-2',6'-diisopropoxybiphenyl s seconds sat. saturation Selectfluor 1-chloromethyl-4-fluoro-1,4-diazoniabicyclo[2.2.2]octane bis(tetrafluoroborate) SM starting material soln. solution SPhos 2-Dichlorohexylphosphino-2',6'-dimethoxybiphenyl TBAF Tetrabutylammonium Fluoride TBDMSCl tert-butyldimethylsilyl chloride TBME tert-butyl methyl ether tBu tert-butyl = tertiary butyl TEA Triethylamine Tf Trifluoromethanesulfonyl TFA trifluoroacetic acid THF tetrahydrofuran TLC thin layer chromatography TMEDA N,N,N',N'-Tetramethylethylenediamine TMS trimethylsilyl Tosyl p-toluenesulfonyl T3P n-propylphosphonic anhydride t R retention time Triflate Trifluoromethanesulfonate pTsOH p-toluenesulfonic acid Xantphos 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene XPhos 2-Dicyclohexylphosphine-2',4',6'-triisopropylbiphenyl

[0191] 2-(4-Methoxy-2H-1,2,3-triazol-2-yl)ethan-1-amine hydrochloride (AM-1)

[0192] Step 1: K2CO3 (22.1 g, 160 mmol) is added to a suspension of 4,5-dibromo-2H-1,2,3-triazole (30.2 g, 133 mmol) and tert-butyl (2-bromoethyl)carbamate (33.5 g, 146 mmol) in MeCN (300 mL) at RT, and the resulting mixture is stirred at 50 °C for 65 h. The RM is partitioned between water and EtOAc, and the layers are separated. The aqueous phase is re-extracted with EtOAc (2x), and the combined organic extracts are washed with brine, dried (MgSO4), filtered, and evaporated. The crude product is purified by FC (eluting with 20% EtOAc in hept) to give tert-butyl (2-bromoethyl)carbamate. Thiol (2-(4,5-dibromo-2H-1,2,3-triazol-2-yl)ethyl)carbamate is obtained as a colorless oil. LC-MS B:t R =0.95min;[M+H] + =370.73.

[0193] Step 2: TFA (51.7 mL, 676 mmol) is added to a solution of tert-butyl (2-(4,5-dibromo-2H-1,2,3-triazol-2-yl)ethyl)carbamate (5.0 g, 13.5 mmol) in DCM (75 mL) at RT, and the RM is stirred for 1 h before being concentrated in vacuo. The residue is co-evaporated with DCM (2x) to give 2-(4,5-dibromo-2H-1,2,3-triazol-2-yl)ethan-1-amine.TFA as a white solid. LC-MS I:t R =0.66 min; [M+H] + =270.94.

[0194] Step 3: Sodium acetate (7.0 g, 85.4 mmol) followed by benzaldehyde (3.83 mL, 37.6 mmol) was added to a solution of 2-(4,5-dibromo-2H-1,2,3-triazol-2-yl)ethan-1-amine. TFA (6.56 g, 17.1 mmol) in MeOH (90 mL) at RT, and the resulting mixture was stirred for 1 h, followed by the addition of NaBH3CN (2.49 g, 37.6 mmol) in portions. The RM was stirred for 48 h and then concentrated. The residue was partitioned between water and DCM, and the layers were separated. The aqueous phase is re-extracted with DCM (2x) and the combined organic extracts are washed with brine, dried (Na2SO4), filtered and evaporated to give N,N-dibenzyl-2-(4,5-dibromo-2H-1,2,3-triazol-2-yl)ethan-1-amine as a yellow solid. LC-MS I:t R = 1.38 min; [M+H] + =450.99.

[0195] Step 4: i PrMgCl (2.0 M in THF, 13.4 mL, 26.8 mmol) is added dropwise to a solution of N,N-dibenzyl-2-(4,5-dibromo-2H-1,2,3-triazol-2-yl)ethan-1-amine (6.03 g, 13.4 mmol) in THF (120 mL) at −78 °C, and the resulting mixture is stirred for 4 h. The RM is warmed to RT and stirred for 30 min before being quenched by the careful addition of saturated aqueous NH₄Cl. The volatiles are evaporated, and the remaining aqueous phase is extracted with EtOAc (3x). The combined organic extracts are washed with brine, dried (Na₂SO₄), filtered, and evaporated to give N,N-dibenzyl-2-(4-bromo-2H-1,2,3-triazol-2-yl)ethan-1-amine as a yellow oil. LC-MS I:t R = 1.30 min; [M+H] + =373.07.

[0196] Step 5: A mixture of N,N-dibenzyl-2-(4-bromo-2H-1,2,3-triazol-2-yl)ethan-1-amine (5.27 g, 14.2 mmol), bis(pinacolato)diboron (5.41 g, 21.3 mmol), SPhos (233 mg, 0.57 mmol), tris(dibenzylideneacetone)dipalladium(0) (134 mg, 0.14 mmol), and KOAc (2.40 g, 24.2 mmol) in dioxane (110 mL) is degassed and inerted with Argon. The RM is then heated to 100 °C for 3 d. The RM is cooled to RT and filtered through Celite, rinsing with EtOAc. The volatiles are evaporated, the residue is partitioned between water and EtOAc, and the layers are separated. The aqueous phase is re-extracted with EtOAc (2x) and the combined organic extracts are washed with brine, dried (Na2SO4), filtered and evaporated to give (2-(2-(dibenzylamino)ethyl)-2H-1,2,3-triazol-4-yl)boronic acid as the major product as a yellow oil. LC-MS I:t R =0.58min;[M+H] + =337.22.

[0197] Step 6: Sodium perborate tetrahydrate (4.72 g, 29.7 mmol) was dissolved in (2-(2-(dibenzylamino)ethyl)-2H-1,2,3-triazol-4-yl)boronic acid (5.0 g, 14.9 mmol) in 1:1 THF:HO (150 mL) at RT and the resulting suspension is stirred for 16 h. The RM is poured into cold water and extracted with EtOAc (3x). The combined organic extracts are washed with brine, dried (MgSO4), filtered and evaporated. The crude product is purified by FC (eluting with 20% to 80% EtOAc in hept) to give 2-(2-(dibenzylamino)ethyl)-2H-1,2,3-triazol-4-ol as a yellow solid. LC-MS I:t R =0.56 min; [M+H] + =309.17. 1H NMR(DMSO) δ:10.30(s, 1H), 7.21-7.31(m, 10H), 7.02(s, 1H), 4.29(t, J=6.3Hz, 2H), 3.55(s, 4H), 2.80(t, J=6.3Hz, 2H).

[0198] Step 7: A 60% dispersion of NaH in mineral oil (540 mg, 11.2 mmol) is added to a solution of 2-(2-(dibenzylamino)ethyl)-2H-1,2,3-triazol-4-ol (3.15 g, 10.2 mmol) in DMF (85 mL) at RT. After stirring for 10 min, MeI (0.77 mL, 12.3 mmol) is added and the resulting mixture is stirred for 1 h. The RM is poured into water and extracted with iPrOAc (3x). The combined organic extracts are washed with brine, dried (Na2SO4), filtered and evaporated to give N,N-dibenzyl-2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethan-1-amine as a yellow oil. LC-MS I:t R = 1.23 min; [M+H] + =323.21.

[0199] Step 8: A solution of N,N-dibenzyl-2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethan-1-amine (3.45 g, 10.7 mmol) in EtOH (85 mL) at RT is inactivated with N2 / vacuum (3x) before adding 10% Pd / C (1.13 g, 1.1 mmol). After three additional inactivations, a H2 balloon is attached and the resulting mixture is stirred for 24 h. The RM is filtered through a Whatman filter, and the filtrate is acidified with 4 M HCl in dioxane (8 mL, 32 mmol) and concentrated to give the title compound AM-1 as a white solid. LC-MS I:t R =0.39 min; [M+H] + =143.20. 1 H NMR (DMSO) δ:8.40(s, 3H), 7.41(s, 1H), 4.54(t, J=6.4Hz, 2H), 3.87(s, 3H), 3.29-3.23(m, 2H).

[0200] 2-(3-Methoxy-1,2,4-oxadiazol-5-yl)ethan-1-amine hydrochloride (AM-2)

[0201] Step 1: HATU (11.82 g, 31.1 mmol) is added to a solution of boc-beta-Ala-OH (5.0 g, 25.9 mmol), o-methylisourea bisulfate (4.5 g, 25.9 mmol), and DIPEA (18.1 mL, 104 mmol) in DMF (150 mL) at RT, and the RM is stirred for 1.5 h. Water and EtOAc are added to the RM, then the two layers are separated and the aqueous layer is extracted with EtOAc (2x). The combined organic layers are washed with brine, dried (Na2SO4), filtered, and concentrated to give the crude product, which is purified by FC (eluting with 20% to 100% EtOAc in hept) to give tert-butyl (3-((imino(methoxy)methyl)amino)-3-oxopropyl)carbamate as a white solid. LC-MS I:t R =0.64 min; [M+H] + =246.36.

[0202] Step 2: 1,8-diazabicyclo[5.4.0]undec-7-ene (8.96 mL, 59.3 mmol) is added to a solution of tert-butyl (3-((imino(methoxy)methyl)amino)-3-oxopropyl)carbamate (6.19 g, 24.7 mmol) and NBS (10.56 g, 59.3 mmol) in EtOAc (120 mL) at RT and the RM is stirred for 5 h. Additional 1,8-diazabicyclo[5.4.0]undec-7-ene (1.85 mL, 12.4 mmol) and NBS (2.2 g, 12.4 mmol) are added and stirring is continued for 16 h. The suspension is filtered and the filtrate is diluted with water, saturated aqueous NaHCO3 and After washing with brine, it is evaporated to dryness. The crude product is purified by FC (eluting with 20% to 100% EtOAc in hept) to give tert-butyl (2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)carbamate as a colorless oil. LC-MS I:t R =0.75min; [M+H] + =244.33.

[0203] Step 3: 4M HCl in dioxane (0.62 mL, 2.47 mmol) is added to a solution of tert-butyl (2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)carbamate (150 mg, 0.62 mmol) in DCM (2 mL) at RT, and the RM is stirred at RT for 4 days and then at 50° C. for 6 h. The mixture is evaporated to give the title compound AM-2 as a white solid. LC-MS I:t R =0.35min;[M+H] + =144.21.

[0204] 2-(3-Methoxyisoxazol-5-yl)ethan-1-amine hydrochloride (AM-3)

[0205] Step 1: DPPA (1.32 mL, 6.1 mmol) is added dropwise to a solution of 3-(3-methoxyisoxazol-5-yl)propanoic acid (1.0 g, 5.55 mmol) and TEA (0.93 mL, 6.66 mmol) in PhMe (25 mL) at RT, and the RM is heated to 100 °C for 1.5 h. 2-Methylpropan-2-ol (1.06 mL, 11.1 mmol) is added, and the RM is heated under reflux for 16 h. The RM is cooled to RT, partitioned between saturated aqueous NaHCO3 and EtOAc, and the layers are separated. The aqueous phase is re-extracted with EtOAc (2x), and the combined organic extracts are washed with brine, dried over Na2SO4, filtered, and evaporated in vacuo. The crude product is purified by FC (eluting with 0% to 100% EtOAc in hept) to give tert-butyl (2-(3-methoxyisoxazol-5-yl)ethyl)carbamate as a colorless oil. LC-MS F:t R = 1.80 min; [M+H] + =243.1.

[0206] Step 2: 4M HCl in dioxane (13.7 mL, 54.7 mmol) is added to a suspension of tert-butyl (2-(3-methoxyisoxazol-5-yl)ethyl)carbamate (1.33 g, 5.47 mmol) in dioxane (30 mL) at RT, and the resulting mixture is heated to 50° C. for 4 h. The RM is concentrated and co-evaporated with EtO to give the title compound AM-3 as a white solid. LC-MS I:t R =0.42 min; [M+H] + =143.25.

[0207] 2-(4-Fluoro-3-methoxyisoxazol-5-yl)ethan-1-amine hydrochloride (AM-4)

[0208] Step 1: In a microwave tube, phthalic anhydride (354 mg, 2.36 mmol) is added to a suspension of AM-3 (402 mg, 2.25 mmol) and DIPEA (0.47 mL, 2.7 mmol) in dioxane (12 mL) at RT. The tube is sealed and heated to 100 °C for 48 h. Water is added to the RM, the mixture is acidified with 1 M HCl, and the product is extracted with EtOAc, dried (MgSO), filtered, and concentrated to give 2-(2-(3-methoxyisoxazol-5-yl)ethyl)isoindoline-1,3-dione (718 mg) as a white solid. LC-MS B:t R =0.85min;[M+H] + =273.09.

[0209] Step 2: Selectfluor (1.07 g, 2.87 mmol) is added to a solution of 2-(2-(3-methoxyisoxazol-5-yl)ethyl)isoindoline-1,3-dione (710 mg, 2.61 mmol) in tetramethylene sulfone (21.7 mL, 226 mmol) at 40° C. and the RM is heated to 120° C. for 18 h. The solution is cooled to around 50°C, and then the RM is poured into pre-stirred HO (30 mL) followed by EtOAc (10 mL). The two layers are separated and the aqueous layer is re-extracted with EtOAc. The combined organic layers are washed with brine, dried (NaSO), filtered, and concentrated. Purification by prep HPLC (acidic) affords 2-(2-(4-fluoro-3-methoxyisoxazol-5-yl)ethyl)isoindoline-1,3-dione (89 mg, 12%) as a colorless oil. LC-MS B:t R =0.90min;[M+H] + =291.02.

[0210] Step 3: Hydrazine monohydrate (0.222 mL, 2.93 mmol) is added to a solution of 2-(2-(4-fluoro-3-methoxyisoxazol-5-yl)ethyl)isoindoline-1,3-dione (85 mg, 0.293 mmol) in EtOH (3 mL) at RT, and the RM is heated to 80° C. for 1 h. The RM is cooled to RT, and a white precipitate forms. Ether is added, and the solid (by-product) breaks up, then is filtered off and discarded. The filtrate is acidified with 4 M HCl in dioxane and concentrated to give the title compound AM-4 as a white solid, which is used directly in the next step. LC-MS B:t R =0.33min;[M+H] + =161.08.

[0211] 2-(5-cyclopropyl-2H-tetrazol-2-yl)ethan-1-amine hydrochloride (AM-5)

[0212] Step 1: tert-Butyl (2-bromoethyl)carbamate (53.2 g, 233 mmol) is added to a suspension of 5-cyclopropyl-2H-1,2,3,4-tetrazole (24.0 g, 211 mmol) and K2CO3 (35.1 g, 254 mmol) in MeCN (480 mL) at RT, and the RM is heated to 50 °C for 18 h. The RM is concentrated, and the residue is partitioned between water and EtOAc, and the layers are separated. The aqueous phase is re-extracted with EtOAc (2x), and the combined organic extracts are washed with brine, dried (MgSO4), filtered, and evaporated. The crude product is purified by FC (eluting with 20% to 80% EtOAc in hept) to give tert-butyl (2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)carbamate as a colorless oil. LC-MS I:t R =0.80min;[M+H] + =254.35.

[0213] Step 2: 4M HCl in dioxane (290 mL, 1.16 mol) is added to a suspension of tert-butyl (2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)carbamate (29.45 g, 116 mmol) in dioxane (630 mL) at RT, and the resulting mixture is stirred for 4 d. The RM is concentrated and co-evaporated with EtO to give the title compound AM-5 (1.36 g, 94%) as a white solid. LC-MS I:t R =0.44 min; [M+H] + =154.25.

[0214] tert-Butyl (R)-(1-hydroxy-3-(6-methoxypyridin-2-yl)propan-2-yl)carbamate (AL-1) Step 1: nBuLi (1.6 M in hex, 18.2 mL, 29.1 mmol) is added dropwise to a solution of (S)-2-isopropyl-3,6-dimethoxy-2,5-dihydropyrazine (4.70 g, 24.2 mmol) in THF (200 mL) at −78° C., and the RM is stirred for 30 min before adding a solution of 2-(bromomethyl)-6-methoxypyridine (5.0 g, 24.2 mmol) in THF (20 mL) dropwise. The RM is stirred for 10 min before being quenched with saturated aqueous NH4Cl. After warming to RT, the RM is diluted with water and extracted with EtOAc (3×). The combined organic extracts are washed with brine, dried (MgSO4), filtered, and evaporated. The crude product was purified by FC (eluting with 30% to 100% EtOAc in hept) to give (2S,5R)-2-isopropyl-3,6-dimethoxy-5-((6-methoxypyridin-2-yl)methyl)-2,5-dihydropyrazine as a yellow solid. LC-MS B:t R =0.83 min; [M+H] + =306.03.

[0215] Step 2: 1M aqueous HCl (38.2 mL, 38.2 mmol) is added to a solution of (2S,5R)-2-isopropyl-3,6-dimethoxy-5-((6-methoxypyridin-2-yl)methyl)-2,5-dihydropyrazine (5.8 g, 19.1 mmol) in MeCN (50 mL) at RT, and the resulting mixture is stirred for 16 h. Partition the RM between saturated aqueous NaHCO3 and DCM, and separate the layers. The aqueous phase is re-extracted with DCM (2x), and the combined organic extracts are washed with brine, dried (Na2SO4), filtered, and evaporated. The crude product is purified by FC (eluting with 80%-100% EtOAc in hept) to give methyl (R)-2-amino-3-(6-methoxypyridin-2-yl)propanoate as a yellow oil. LC-MS B:t R =0.46 min; [M+H] + =211.26.

[0216] Step 3: BocO (2.61 g, 12.0 mmol) is added to a suspension of methyl (R)-2-amino-3-(6-methoxypyridin-2-yl)propanoate (2.28 g, 10.8 mmol) and NaHCO (4.56 g, 54.2 mmol) in a mixture of THF (50 mL) and HO (50 mL) at RT, and the RM is stirred for 1 h. Acidify with aqueous HCl and extract with EtOAc (3x). Combine the organic extracts, dry (MgSO4), filter, and concentrate to give methyl (R)-2-((tert-butoxycarbonyl)amino)-3-(6-methoxypyridin-2-yl)propanoate as a colorless oil. LC-MS B:t R =0.89 min; [M+H] + =311.25.

[0217] Step 4: LAH (906 mg, 22.7 mmol) is added portionwise to a solution of methyl (R)-2-((tert-butoxycarbonyl)amino)-3-(6-methoxypyridin-2-yl)propanoate (3.52 g, 11.3 mmol) in EtO (150 mL) at 0 °C, and the RM is warmed to RT and stirred for 1 h. The RM is recooled to 0 °C and very carefully quenched with H O, followed by the addition of EtOAc. The resulting suspension is warmed to RT and filtered. The layers are separated, and the aqueous layer is re-extracted with EtOAc (2x). The combined organic extracts are washed with brine, dried (NaSO), filtered, and evaporated to give the title compound AL-1 as a colorless oil. LC-MS B:t R =0.62 min; [M+H] + =283.35.

[0218] tert-Butyl (R)-(1-(6-bromopyridin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-2) The title compound is prepared following the reaction sequence described for AL-1, substituting 2-bromo-6-(bromomethyl)pyridine for 2-(bromomethyl)-6-methoxypyridine in step 1. LC-MS B:t R =0.76 min; [M+H] +=331.16.

[0219] tert-Butyl (R)-(1-(6-bromo-5-fluoropyridin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-3) The title compound is prepared following the reaction sequence described for AL-1, substituting 2-bromo-6-(bromomethyl)-3-fluoropyridine for 2-(bromomethyl)-6-methoxypyridine in step 1. LC-MS I:t R =0.84 min; [M+H] + =349.05.

[0220] tert-Butyl (R)-(1-hydroxy-3-(oxazol-4-yl)propan-2-yl)carbamate (AL-4) The title compound is prepared following the reaction sequence described for AL-1, substituting 4-(bromomethyl)oxazole for 2-(bromomethyl)-6-methoxypyridine in step 1. LC-MS J:t R =1.54 min;[M+H- t Bu] + =187.0.

[0221] tert-Butyl (R)-(1-(4,6-dimethoxypyrimidin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-5) The title compound is prepared following the reaction sequence described for AL-1, substituting 2-(chloromethyl)-4,6-dimethoxypyrimidine for 2-(bromomethyl)-6-methoxypyridine in step 1. LC-MS I:t R =0.75min; [M+H] + =314.11.

[0222] tert-Butyl (R)-(1-(6-bromo-4-methoxypyridin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-6)

[0223] Step 1: Zinc dust (1.77 g, 26.5 mmol) is heated to 140 °C under vacuum for 30 min, then cooled to RT under argon. Iodine (2.02 g, 7.96 mmol) and DMF (10 mL) are added, and the resulting mixture is stirred for 20 min, after which Boc-3-iodo-D-Ala-OMe (3.0 g, 8.84 mmol) is added and stirring is continued for an additional 20 min. 2,6-Dibromo-4-methoxypyridine (3.07 g, 11.5 mmol) and PdCl2(PPh3)2 (310 mg, 0.44 mmol) are added, and the RM is stirred at 50 °C for 16 h. The RM is partitioned between water and EtOAc and filtered. The layers are separated, and the aqueous layer is re-extracted with EtOAc (2x). The combined organic extracts are washed with brine, dried (Na2SO4), filtered, and evaporated. The crude product is purified by FC (eluting with 40% EtOAc in hept) to give methyl (R)-3-(6-bromo-4-methoxypyridin-2-yl)-2-((tert-butoxycarbonyl)amino)propanoate as a colorless oil. LC-MS B:t R =0.92 min; [M+H] + =389.25.

[0224] Step 2: The title compound is prepared from methyl (R)-3-(6-bromo-4-methoxypyridin-2-yl)-2-((tert-butoxycarbonyl)amino)propanoate according to the procedure described for AL-1, Step 4. LC-MS B:t R =0.78 min; [M+H] + =361.16.

[0225] tert-Butyl (R)-(1-hydroxy-3-(6-methoxy-4-methylpyridin-2-yl)propan-2-yl)carbamate (AL-7) The title compound is prepared following the reaction sequence described for AL-6, substituting 2-bromo-6-methoxy-4-methylpyridine for 2,6-dibromo-4-methoxypyridine in step 1. LC-MS I:t R =0.84 min; [M+H] + =297.22.

[0226] tert-Butyl (R)-(1-(4,6-dimethoxypyridin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-8) The title compound is prepared following the reaction sequence described for AL-6, substituting 2-bromo-4,6-dimethoxypyridine for 2,6-dibromo-4-methoxypyridine in step 1. LC-MS B:t R =0.55min;[M+H] + =313.24.

[0227] tert-Butyl (R)-(1-(6-bromo-4-methylpyridin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-9) The title compound was prepared by reacting 2,6-dibromo-4-methoxypyridine with 2,6-dibromo-4-methoxypyridine in step 1. Prepared following the reaction sequence described for AL-6, substituting bromo-4-methylpyridine. LC-MS I:t R =0.84 min; [M+H] + =347.17.

[0228] tert-Butyl (R)-(1-hydroxy-3-(3-methoxyphenyl)propan-2-yl)carbamate (AL-10) The title compound is prepared from Boc-3-methoxy-D-phenylalanine according to the procedure described for AL-1, step 4. LC-MS I:t R =0.83min;[M+H- t Bu] + =226.26.

[0229] tert-Butyl (R)-(1-hydroxy-3-(6-methoxypyrazin-2-yl)propan-2-yl)carbamate (AL-11) The title compound is prepared following the reaction sequence described for AL-1, substituting 2-(chloromethyl)-6-methoxypyrazine for 2-(bromomethyl)-6-methoxypyridine in step 1. LC-MS B:t R=0.70 min; [M+H] + =284.25.

[0230] Benzyl (S)-6-(2-((tert-butoxycarbonyl)amino)-3-iodopropoxy)-3-fluoroquinoline-5-carboxylate (IM-1)

[0231] Step 1: DIAD (4.41 mL, 22.4 mmol) is added to a solution of benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (4.76 g, 16.0 mmol), tert-butyl (S)-4-(hydroxymethyl)-2,2-dimethyloxazolidine-3-carboxylate (5.0 g, 21.1 mmol), and PPh3 (6.3 g, 24.0 mmol) in THF (100 mL) at RT, and the RM is stirred for 1 h. The RM is partitioned between water and EtOAc, and the layers are separated. The aqueous phase is re-extracted with EtOAc (2x), and the combined organic extracts are washed with brine, dried (MgSO4), filtered, and evaporated. The crude product is purified by FC (eluting with 20% to 80% EtOAc in hept) to give tert-butyl (S)-4-(((5-((benzyloxy)carbonyl)-3-fluoroquinolin-6-yl)oxy)methyl)-2,2-dimethyloxazolidine-3-carboxylate as a colorless oil. LC-MS B:t R = 1.22 min; [M+H] + =511.31.

[0232] Step 2: 4M HCl in dioxane (27 mL, 108 mmol) is added to a solution of tert-butyl (S)-4-(((5-((benzyloxy)carbonyl)-3-fluoroquinolin-6-yl)oxy)methyl)-2,2-dimethyloxazolidine-3-carboxylate (5.51 g, 10.8 mmol) in dioxane (60 mL) at RT and the RM is heated to 50° C. for 2 days. The mixture is evaporated and triturated with EtO to give benzyl (R)-6-(2-amino-3-hydroxypropoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride as a white solid. LC-MS B:t R =0.69 min; [M+H]+ =371.26.

[0233] Step 3: A solution of BocO (2.81 g, 12.6 mmol) in DCM (5 mL) is added dropwise to a suspension of benzyl (R)-6-(2-amino-3-hydroxypropoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (5.32 g, 12.0 mmol) and TEA (8.35 mL, 60 mmol) in DCM (40 mL) at 0 °C, and the RM is warmed to RT and stirred for 3 h. The RM is acidified with 1 M aqueous citric acid and the layers are separated. The aqueous phase is re-extracted with DCM (2x), and the combined organic extracts are washed with brine, dried (NaSO), filtered, and evaporated to give benzyl (R)-6-(2-((tert-butoxycarbonyl)amino)-3-hydroxypropoxy)-3-fluoroquinoline-5 -carboxylate as a yellow oil. LC-MS B:t R = 1.03 min; [M+H] + =471.31.

[0234] Step 4: Iodine beads (1-3 mm, 2.37 g, 9.35 mmol) are added portionwise to a solution of PPh3 (2.58 g, 9.35 mmol) and imidazole (637 mg, 9.35 mmol) in DCM (45 mL) at RT, and the resulting mixture is stirred for 15 min, after which a solution of benzyl (R)-6-(2-((tert-butoxycarbonyl)amino)-3-hydroxypropoxy)-3-fluoroquinoline-5-carboxylate in DCM (19 mL) is added dropwise and stirring is continued for 3 h. The RM is partitioned between saturated aqueous NaHCO3 and DCM, and the layers are separated. The aqueous phase is re-extracted with DCM (2x), and the combined organic extracts are washed with brine, dried (Na2SO4), filtered, and evaporated. The crude product is purified by FC (eluting with 20% to 80% EtOAc in hept) to give the title compound IM-1 as a yellow oil. LC-MS B:t R = 1.18 min; [M+H] + =581.19.

[0235] tert-Butyl (S)-3-amino-4-((2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)amino)-4-oxobutanoate (A-1)

[0236] Step 1: HATU (3.20 g, 8.42 mmol) was added to a solution of Fmoc-L-aspartic acid beta-tert-butyl ester (3.54 g, 8.42 mmol), 2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethan-1-amine hydrochloride (AM-1, 1.6 g, 8.42 mmol), and DIPEA (6.34 mL, 33.7 mmol) in DMF (27 mL) at RT, and the RM was stirred for 1 h. The RM was partitioned between water and EtOAc, and the layers were separated. The aqueous phase is re-extracted with EtOAc (2x) and the combined organic extracts are washed with saturated aqueous NaHCO3, brine, dried over Na2SO4, filtered and evaporated to give tert-butyl (S)-3-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-4-((2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)amino)-4-oxobutanoate as a white solid. LC-MS I:t R = 1.10 min; [M+H] + =536.28.

[0237] Step 2: Piperidine (4.29 mL, 42.9 mmol) is added to a solution of tert-butyl (S)-3-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-4-((2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)amino)-4-oxobutanoate (5.29 g, 8.58 mmol) in DCM (67 mL) at RT and the RM is stirred for 3 h. The RM is concentrated and the residue is directly purified by FC (eluting with 10% MeOH in DCM) to give the title compound A-1 as a yellow oil. LC-MS I:t R =0.65min;[M+H] + =314.27.

[0238] Table 1-A below lists component A, which is prepared similarly to the two-step sequence described above for A-1.

[0239] [Table 3]

[0240] Benzyl (R)-6-(2-amino-3-phenylpropoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (B-1)

[0241] Step 1: A solution of Br2 (0.17 mL, 3.37 mmol) in AcOH (8.0 mL) is added to a solution of 3-fluoroquinolin-6-ol (0.50 g, 3.06 mmol) and NaOAc (0.30 g, 3.68 mmol) in AcOH (20 mL) at RT, and the RM is stirred for 30 min. The RM is concentrated to dryness, and the residue is partitioned and extracted between saturated aqueous NaHCO3 and EtOAc. The layers are separated, and the aqueous layer is re-extracted with EtOAc (2x). The combined organic extracts are washed with brine, dried (Na2SO4), filtered, and evaporated to give 5-bromo-3-fluoroquinolin-6-ol as a brown solid. LC-MS J:t R = 1.38 min; [M+H] + =239.9.

[0242] Step 2: A solution of 5-bromo-3-fluoroquinolin-6-ol (0.74 g, 3.06 mmol) in THF (15 mL) is added dropwise to a suspension of 60% NaH in mineral oil dispersion (0.17 g, 4.29 mmol) in THF (15 mL) at RT. The resulting mixture is stirred for 15 min, after which methoxymethyl bromide (0.3 mL, 3.67 mmol) is added dropwise at 0 °C. After stirring at 0 °C for 1.5 h, the RM is quenched by the addition of HO and extracted with EtOAc. The organic layer is washed with NaHCO , brine, dried (Na SO ), filtered, and evaporated. The crude product is purified by FC (eluting with 2% to 30% EtOAc in hept) to give 5-bromo-3-fluoro-6-(methoxymethoxy)quinoline as a colorless oil. LC-MS J:t R = 2.03 min; non-ionized.

[0243] Step 3: nBuLi (1.6 M in hex, 0.98 mL, 1.57 mmol) is added dropwise to a solution of 5-bromo-3-fluoro-6-(methoxymethoxy)quinoline (300 mg, 1.05 mmol) in THF (18 mL) at −78° C., and the RM is stirred for 30 min. The RM is quenched with freshly crushed dry ice (1.0 g, 22.7 mmol), then warmed to RT and stirred for 30 min. The RM is concentrated in vacuo, and the intermediate lithium carboxylate is dissolved in DMF (4 mL), followed by the addition of KHCO (31.5 mg, 0.315 mmol) and BnBr (0.15 mL, 1.26 mmol), and the RM is stirred at RT for 1 h. Stir for 6 h. Partition the RM between saturated aqueous NaHCO3 and EtOAc and extract. Separate the layers and re-extract the aqueous layer with EtOAc (2x). Combine the organic extracts, wash with brine, dry (Na2SO4), filter, and evaporate. Purify the crude product by prep. HPLC (basic) to give benzyl 3-fluoro-6-(methoxymethoxy)quinoline-5-carboxylate as a yellow oil. LC-MS J:t R = 2.08 min; [M+H] + =342.10.

[0244] Step 4: TFA (0.24 mL, 3.13 mmol) is added to a solution of benzyl 3-fluoro-6-(methoxymethoxy)quinoline-5-carboxylate (107 mg, 0.31 mmol) in DCM (3 mL) at RT, and the resulting mixture is stirred for 2 h. The RM is concentrated in vacuo, and the residue is dissolved in EtOAc and extracted with saturated aqueous NaHCO3. The aqueous layer is extracted with EtOAc, and the combined organic extracts are washed with brine, dried (NaSO4), filtered, and concentrated to give benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate as a light brown oil. LC-MS J:t R = 2.06 min; [M+H] + =298.1.

[0245] Step 5: DIAD (0.064 mL, 0.33 mmol) is added to a mixture of benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (93.8 mg, 0.31 mmol), tert-butyl (R)-(1-hydroxy-3-phenylpropan-2-yl)carbamate (82 mg, 0.33 mmol), and PPh3 (86 mg, 0.33 mmol) in THF (2 mL) at 0 °C, and the RM is stirred at RT for 16 h. The mixture is concentrated, and the residue is directly purified by FC (eluting with 20% to 60% EtOAc in hept) to give benzyl (R)-6-(2-((tert-butoxycarbonyl)amino)-3-phenylpropoxy)-3-fluoroquinoline-5-carboxylate as a colorless oil. LC-MS J:t R = 2.39 min; [M+H] + =531.2.

[0246] Step 6: 4M HCl in dioxane (0.44 mL, 1.77 mmol) was added to benzyl A solution of (R)-6-(2-((tert-butoxycarbonyl)amino)-3-phenylpropoxy)-3-fluoroquinoline-5-carboxylate (94 mg, 0.18 mmol) in dioxane (3 mL) is added and the RM is stirred at RT for 24 h. The volatiles are removed in vacuo and the residue is triturated with EtO (3x) to give the title compound B-1 as a white solid. LC-MS J:t R = 2.10 min; [M+H] + =431.2.

[0247] Benzyl (R)-6-(2-amino-3-(6-methoxypyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (B-2) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-hydroxy-3-(6-methoxypyridin-2-yl)propan-2-yl)carbamate (AL-1) according to steps 5 and 6 described for B-1. LC-MS B:t R =0.83 min; [M+H]+ =462.29.

[0248] Benzyl (R)-6-(2-amino-3-(6-bromopyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (B-3) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-(6-bromopyridin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-2) according to steps 5 and 6 described for B-1. LC-MS B:t R =0.83 min; [M+H] + =510.17.

[0249] Benzyl (R)-6-(2-amino-3-(6-bromo-5-fluoropyridine-2- (R)-(1-(6-bromo-5-fluoropyridin-2-yl)-3-hydroxypropan-2-yl)carbamate dihydrochloride (B-4) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-(6-bromo-5-fluoropyridin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-3) according to steps 5 and 6 described for B-1. LC-MS I:t R = 1.09 min; [M+H] + =530.06. Note: As a by-product, benzyl Also contains (R)-6-(2-amino-3-(6-chloro-5-fluoropyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride. LC-MS I:t R = 1.08 min; [M+H] + =484.13.

[0250] Benzyl (R)-6-(2-amino-3-(oxazol-4-yl)propoxy)-3-fluoroquinoline-5-carboxylate 2,2,2-trifluoroacetate (B-5) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-hydroxy-3-(oxazol-4-yl)propan-2-yl)carbamate (AL-4) according to steps 5 and 6 described for B-1, using TFA instead of HCl for Boc deprotection. LC-MS J:t R = 1.88 min; [M+H] + =422.2.

[0251] Benzyl (R)-6-(2-amino-3-(6-methylpyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (B-6)

[0252] Step 1: Zinc dust (30.0 mg, 0.45 mmol) is heated to 120° C. under vacuum for 20 min, then cooled to 70° C. under argon. Iodine (2.8 mg, 0.011 mmol) and DMF (1.0 mL) are added, and the resulting mixture is stirred for 20 min. After cooling to 50° C., a solution of benzyl (S)-6-(2-((tert-butoxycarbonyl)amino)-3-iodopropoxy)-3-fluoroquinoline-5-carboxylate (IM-1) (130 mg, 0.22 mmol) in DMF (1.0 mL) is added, and stirring is continued for an additional 20 min. 2-Bromo-6-methylpyridine (33.8 μL, 0.291 mmol), Pd2(dba)3 (10.3 mg, 0.011 mmol), and XPhos (21.4 mg, 0.045 mmol) are added and the RM is stirred at 50 °C for 2 h. The RM is partitioned between water and EtOAc and filtered through a Whatman filter. The layers are separated and the aqueous layer is re-extracted with EtOAc (2x). The combined organic extracts are washed with brine, dried (Na2SO4), filtered, and evaporated. The crude product is purified by prep. HPLC (basic) to give benzyl (R)-6-(2-((tert-butoxycarbonyl)amino)-3-(6-methylpyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate as a colorless oil. LC-MS B:t R =0.88min;[M+H] +=546.14.

[0253] Step 2: 4M HCl in dioxane (1.5 mL, 1.58 mmol) is added to a solution of benzyl (R)-6-(2-((tert-butoxycarbonyl)amino)-3-(6-methylpyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate (43 mg, 0.079 mmol) in dioxane (3 mL) at RT, and the RM is stirred at RT for 16. The mixture is evaporated to give the title compound B-6 as a white solid. LC-MS B:t R =0.70 min; [M+H] + =446.28.

[0254] Benzyl (R)-6-(2-amino-3-(5-fluoro-6-methylpyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (B-7) The title compound was prepared by the reaction of benzyl (S)-6-(2-((tert-butoxycarbonyl)amino)-3-iodopropoxy)-3-fluoroquinoline-5-carboxylate (IM- Prepared from 1) and 2-bromo-5-fluoro-6-methylpyridine according to the procedure described for B-6. LC-MS B:t R =0.84 min; [M+H] + =564.19.

[0255] Benzyl (R)-6-(2-amino-3-(4,6-dimethoxypyrimidin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate bis(2,2,2-trifluoroacetate) (B-8) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-(4,6-dimethoxypyrimidin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-5) according to steps 5 and 6 described for B-1, using TFA instead of HCl for Boc deprotection. LC-MS I:t R = 1.02 min; [M+H] +=493.22.

[0256] Benzyl (R)-6-(2-amino-3-(4,6-dimethoxypyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate bis(2,2,2-trifluoroacetate) (B-9) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-(4,6-dimethoxypyridin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-8) according to steps 5 and 6 described for B-1, using TFA instead of HCl for Boc deprotection. LC-MS B:t R =0.84 min; [M+H] + =492.25.

[0257] Benzyl (R)-6-(2-amino-3-(6-methoxy-4-methylpyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate bis(2,2,2-trifluoroacetate) (B-10) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-hydroxy-3-(6-methoxy-4-methylpyridin-2-yl)propan-2-yl)carbamate (AL-7) according to steps 5 and 6 described for B-1, using TFA instead of HCl for Boc deprotection. LC-MS I:t R = 1.07 min; [M+H] + =476.20.

[0258] Benzyl (R)-6-(2-amino-3-(6-bromo-4-methoxypyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (B-11) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-(6-bromo-4-methoxypyridin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-6) according to steps 5 and 6 described for B-1. LC-MS I:t R = 1.05 min; [M+H] + =540.24.

[0259] Benzyl (R)-6-(2-amino-3-(4-methoxy-6-methylpyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (B-12)

[0260] Step 1: Pd(dppf)Cl.sub.2·DCM (4.0 mg, 0.005 mmol) and 2 M ZnMe.sub.2 in toluene (0.36 mL, 0.72 mmol) were reacted with benzyl (R)-6-(3-(6-bromo-4-methoxypyridin-2-yl)-2-((tert-butoxy)-2-yl)-2-methylpropional to prepare a 2 M ZnMe.sub.2 toluene-based ... A solution of (R)-6-(2-((tert-butoxycarbonyl)amino)propoxy)-3-fluoroquinoline-5-carboxylate (B-11, step-1) (210 mg, 0.33 mmol) in dioxane (6 mL) at RT is added and the RM is heated to 80° C. for 4 h. The RM is partitioned between water and EtOAc and filtered through a Whatman filter. The layers are separated and the aqueous layer is re-extracted with EtOAc (2×). The combined organic extracts are washed with brine, dried (Na2SO4), filtered and evaporated. The crude product is purified by prep. HPLC (basic) to give benzyl (R)-6-(2-((tert-butoxycarbonyl)amino)-3-(4-methoxy-6-methylpyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate as a colorless oil. LC-MS B:t R =0.90min;[M+H] + =576.36.

[0261] Step 2: The title compound is prepared from benzyl (R)-6-(2-((tert-butoxycarbonyl)amino)-3-(4-methoxy-6-methylpyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate according to the procedure described for B-6, Step 2. LC-MS B:t R =0.65min;[M+H] + =476.30.

[0262] Benzyl (R)-6-(2-amino-3-(6-bromo-4-methylpyridin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (B-13) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-(6-bromo-4-methylpyridin-2-yl)-3-hydroxypropan-2-yl)carbamate (AL-9) according to steps 5 and 6 described for B-1. LC-MS I:t R = 1.09 min; [M+H] + =524.22.

[0263] Benzyl (R)-6-(2-amino-3-(3-methoxyphenyl)propoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (B-14) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-hydroxy-3-(3-methoxyphenyl)propan-2-yl)carbamate (AL-10) according to steps 5 and 6 described for B-1. LC-MS I:t R = 1.07 min; [M+H] + =461.32.

[0264] Benzyl (R)-6-(2-amino-3-phenylpropoxy)-3-methylisoquinoline-5-carboxylate dihydrochloride (B-15)

[0265] Step 1: Trifluoromethanesulfonic anhydride (26.2 mL, 158 mmol) is added dropwise to a solution of 2-hydroxy-4-methoxybenzaldehyde (16 g, 105 mmol) and pyridine (42.5 mL, 526 mmol) in DCM (70 mL) at −10° C., and the RM is stirred for 30 min. The RM is quenched with ice water, acidified with 1 M aqueous HCl, and then extracted with EtOAc (2×). The combined organic extracts are washed with brine, dried over NaSO, filtered, and evaporated in vacuo to give 2-formyl-5-methoxyphenyl trifluoromethanesulfonate as a yellow oil. 1 H NMR (400MHz, CDCl3) δ 10.13(s, 1H), 7.95(d, J=8.8Hz, 1H), 7.03(dd, J=8.7, 2.3Hz, 1H), 6.88(d, J=2.3Hz, 1H), 3.93(s, 3H).

[0266] Step 2: A solution of 2-formyl-5-methoxyphenyl trifluoromethanesulfonate (19.6 g, 66.4 mmol) and TEA (93 mL, 664 mmol) in DMF (400 mL) at RT is purged with Ar for 30 min. (1M in heptane, 133 mL, 133 mmol), CuI (1.27 g, 6.64 mmol), and Pd(PPh3)4 (5.0 g, 4.33 mmol) are added sequentially, and the RM is sealed and stirred for 2 h. The RM is filtered through a pad of Celite, and the filtrate is partially concentrated in vacuo, then diluted with EtOAc, washed successively with 1M KHSO4 solution and brine, and concentrated in vacuo. The crude product is purified by FC (eluting with 0% to 30% EtOAc in heptane) to give 4-methoxy-2-(prop-1-yn-1-yl)benzaldehyde as a yellow solid. LC-MS J:t R = 1.80 min; [M+H] + =175.1.

[0267] Step 3: A solution of 4-methoxy-2-(prop-1-yn-1-yl)benzaldehyde (10.3 g, 58.8 mmol) in MeOH (350 mL) at RT is purged with Ar in an autoclave for 5 min. NH3 7M in MeOH (150 mL, 1050 mmol) is added and the RM is heated to 65 °C for 4 h under a pressure reaching 2 bar. The RM is concentrated in vacuo and the residue is co-evaporated with DCM (2x) to give 6-methoxy-3-methylisoquinoline as a brown solid. LC-MS J:t R = 1.81 min; [M+H] + =174.1.

[0268] Step 4: BBr3 (1 M in DCM, 55.4 mL, 55.4 mmol) is added dropwise to a solution of 6-methoxy-3-methylisoquinoline (5.0 g, 27.7 mmol) in DCM (100 mL) at -78 °C. The cooling bath is removed and the RM is stirred at RT for 30 h. The RM is carefully quenched into cold MeOH and concentrated in vacuo. The residue is co-evaporated with PhMe, EtOAc and DCM to give 3-methylisoquinolin-6-ol as a brown solid. LC-MS J:t R = 1.10 min; [M+H] + =160.1.

[0269] Step 5: Br2 (1.3 mL, 25.3 mmol) is added dropwise to a suspension of 3-methylisoquinolin-6-ol (4.67 g, 19.4 mmol) in CHCl3 (75 mL) and the RM is stirred for 2 h. EtOAc is added and the solid is collected by filtration and washed with EtOAc and hept. The filter residue is neutralized by suspending in saturated aqueous NaHCO3, re-filtered and then washed with H2O and hept. The filter residue is suspended in MeCN and evaporated to give 5-bromo-3-methylisoquinolin-6-ol as a brown solid. LC-MS J:t R = 1.02 min; [M+H] + =238.0.

[0270] Step 6: tert-butyl (R)-(1-((5-bromo-3-methylisoquinolin-6-yl)oxy)-3-phenylpropan-2-yl)carbamate is prepared from 5-bromo-3-methylisoquinolin-6-ol and tert-butyl (R)-(1-hydroxy-3-phenylpropan-2-yl)carbamate in analogy to the procedure described for B1 step 5. LC-MS J:t R = 2.21 min; [M+H] + =471.1.

[0271] Step 7: A solution of tert-butyl (R)-(1-((5-bromo-3-methylisoquinolin-6-yl)oxy)-3-phenylpropan-2-yl)carbamate (2.5 g, 5.30 mmol), benzyl alcohol (2.76 mL, 26.5 mmol), and DIPEA (2.78 mL, 15.9 mmol) in PhMe (20 mL) at RT is purged with Ar for 10 min. The RM is then purged with CO and heated to 88 °C under a CO atmosphere before addition of Pd( t A solution of (Bu3P)2 (271 mg, 0.53 mmol) in PhMe (5.5 mL) is added via syringe pump (3 mL / h). The temperature is raised to 95 °C and the RM is stirred under CO atmosphere for 24 h. The RM is cooled to RT and concentrated in vacuo, and the residue is partitioned between saturated aqueous NaHCO3 and EtOAc and extracted. The layers are separated, the aqueous phase is re-extracted with EtOAc (1x), the organic layers are combined, washed with brine, dried over Na2SO4, filtered and evaporated in vacuo. The crude product is purified by FC (eluting with 5%-65% EtOAc in hept) to give benzyl (R)-6-(2-((tert-butoxycarbonyl)- ... (3-phenylpropoxy)-3-methylisoquinoline-5-carboxylate is obtained as a colorless oil. LC-MS J:t R = 2.19 min; [M+H] + =527.2.

[0272] Step 8: The title compound is prepared from benzyl (R)-6-(2-((tert-butoxycarbonyl)amino)-3-phenylpropoxy)-3-methylisoquinoline-5-carboxylate analogously to the procedure described for B1, step 6. LC-MS J:t R = 1.95 min; [M+H] + =427.2.

[0273] Benzyl (R)-6-(2-amino-3-(3-methoxyphenyl)propoxy)-3-methylisoquinoline-5-carboxylate dihydrochloride (B-16) The title compound is prepared similarly to the procedure described for B-15, replacing tert-butyl (R)-(1-hydroxy-3-phenylpropan-2-yl)carbamate with tert-butyl (R)-(1-hydroxy-3-(3-methoxyphenyl)propan-2-yl)carbamate (AL-10) in step 6. LC-MS B:t R =0.66 min; [M+H] + =457.19.

[0274] Benzyl (R)-6-(2-amino-3-(6-methoxypyrazin-2-yl)propoxy)-3-fluoroquinoline-5-carboxylate dihydrochloride (B-17) The title compound is prepared from benzyl 3-fluoro-6-hydroxyquinoline-5-carboxylate (B-1, steps 1-4) and tert-butyl (R)-(1-hydroxy-3-(6-methoxypyrazin-2-yl)propan-2-yl)carbamate (AL-11) according to steps 5 and 6 described for B-1. LC-MS B:t R =0.79 min; [M+H] + =463.30.

[0275] Benzyl (R)-6-(2-amino-3-(6-methoxypyridin-2-yl)propoxy)-8-fluoroquinoline-5-carboxylate dihydrochloride (B-18)

[0276] Step 1: Br2 (1.72 mL, 33.4 mmol) is added dropwise to a suspension of 8-fluoro-6-methoxyquinoline (5.91 g, 33.4 mmol) and NaOAc (3.28 g, 40 mmol) in AcOH (43 mL), and the RM is stirred for 30 min. The reaction is quenched by adding 10% aqueous NaHSO3 and extracted with EtOAc (2x). The combined organic extracts are washed with brine, dried (Na2SO4), filtered, and concentrated. The black residue is heated to 70 °C in MeCN for 2 h before being cooled to 0 °C. The resulting solid is collected by filtration and dried under vacuum to give 5-bromo-8-fluoro-6-methoxyquinoline as a brown solid. LC-MS I:t R =0.86 min; [M+H] + =255.97.

[0277] Step 2: BBr3 (1M in DCM, 4892 mg, 19.5 mmol) is added to a solution of 5-bromo-8-fluoro-6-methoxyquinoline (2000 mg, 7.81 mmol) in DCM (35 mL) at RT. After 68 h of reaction time at RT, LCMS shows complete conversion to the target compound. Methanol is added, taking care to avoid significant heat generation, and a solid forms during this quenching step. This solid is collected by filtration, thoroughly dried, and identified as the desired compound: 1.26 g of 5-bromo-8-fluoroquinolin-6-ol as a light green solid. No further purification is necessary. LCMS I:t R =0.28min; non-ionized.

[0278] Step 3: DIAD (1.39 mL, 6.94 mmol) was treated with 5-bromo-8-fluoroquinolin-6-ol (1200 mg, 4.96 mmol), tert-butyl (R)-(1-hydroxy-3-(6-methoxypyridin-2-yl)propan-2-yl)caproate. To a solution of AL-1 (1540 mg, 5.45 mmol) and PPh3 (1970 mg, 7.44 mmol) in THF (50 mL) at RT is added the carbamate (AL-1, 1540 mg, 5.45 mmol) and PPh3 (1970 mg, 7.44 mmol) in THF (50 mL). The resulting mixture is stirred and heated to 60 °C for 18 h. The mixture is evaporated to dryness and directly purified by FC (eluting with 10% up to 40% EtOAc in heptane) to give the desired product, which still contains the DIAD by-product. This compound is purified by large-scale prep HPLC (basic conditions) to give 750 mg of tert-butyl (R)-(1-((5-bromo-8-fluoroquinolin-6-yl)oxy)-3-(6-methoxypyridin-2-yl)propan-2-yl)carbamate as a pale yellow oil. LC-MS I t R = 1.16 min; [M+H] + =505.96.

[0279] Step 4: tert-Butyl (R)-(1-((5-bromo-8-fluoroquinolin-6-yl)oxy)-3-(6-methoxypyridin-2-yl)propan-2-yl)carbamate (290 mg, 0.573 mmol), benzyl alcohol (0.898 mL, 8.59 mmol), and DIPEA (0.2 mL, 1.15 mmol) are dissolved in anhydrous THF (5.65 mL). The resulting mixture is sonicated until completely dissolved and degassed with N2. In parallel, Pd( tA solution of Bu3P2 (108 mg, 0.206 mmol) in anhydrous THF (6.73 mL) is prepared in another sealed vial. The dark orange solution is manually stirred at RT, and the resulting solution is used immediately: the two solutions are pumped through a 25 mL stainless steel coil on a Vapourtec Flow Chemistry system at 140 °C under ∼20 bar CO2 pressure at a similar flow rate (0.313 mL / min, P ∼12 bar); after a resulting residence time of 40 min in the stainless steel coil, the mixture is cooled and degassed; the combined mixture is concentrated under vacuum. The residue is dissolved in MeCN and filtered through a Wheatman filter to remove remaining palladium(0). The crude product is then first purified by FC (eluting with 10% to 40% EtOAc in Hept) to remove most of the benzyl alcohol, followed by large-scale Prep-HPLC (basic conditions) to give 132 mg of benzyl (R)-6-(2-((tert-butoxycarbonyl)amino)-3-(6-methoxypyridin-2-yl)propoxy)-8-fluoroquinoline-5-carboxylate as a colorless oil. LCMS I:t R = 1.21 min; [M+H] + =561.90.

[0280] Step 5: HCl (4N in dioxane, 1.1 mL, 4.38 mmol) is added to a solution of benzyl (R)-6-(2-((tert-butoxycarbonyl)amino)-3-(6-methoxypyridin-2-yl)propoxy)-8-fluoroquinoline-5-carboxylate (225 mg, 0.43 mmol) in dioxane (2 mL) at RT, and the resulting mixture is stirred for 18 h. The reaction mixture is thoroughly evaporated to dryness to give 216 mg of benzyl (R)-6-(2-amino-3-(6-methoxypyridin-2-yl)propoxy)-8-fluoroquinoline-5-carboxylate dihydrochloride is obtained as a yellow solid. LC-MS I t R =0.97 min; [M+H] + =462.12.

[0281] (R)-1-(N-(tert-butoxycarbonyl)-N-methyl-L-leucyl)piperidine-2-carboxylic acid (C-1)

[0282] Step 1: HATU (7.74 g, 20.3 mmol) is added portionwise to a solution of Boc-N-methyl-L-leucine (5.0 g, 20.3 mmol), (R)-piperidine-2-carboxylic acid methyl ester hydrochloride (3.73 g, 20.3 mmol), and DIPEA (10.4 mL, 61 mmol) in DMF (50 mL) at RT, and the resulting mixture is stirred for 1 h. Water is added, and the mixture is extracted with EtOAc (3x). The combined organic extracts are washed successively with saturated aqueous NaHCO3, water, and brine, dried (Na2SO4), filtered, and concentrated. Purification by FC (eluting with 20% to 50% EtOAc in hept) gave methyl (R)-1-(N-(tert-butoxycarbonyl)-N-methyl-L-leucine). (sil)piperidine-2-carboxylate is obtained as a colorless oil. LC-MS B:t R = 1.05 min; [M+H] + =371.51.

[0283] Step 2: 2M aqueous NaOH (15.0 mL, 29.7 mmol) is added to a solution of methyl (R)-1-(N-(tert-butoxycarbonyl)-N-methyl-L-leucyl)piperidine-2-carboxylate (5.51 g, 14.9 mmol) in MeOH (45 mL) at RT, and the mixture is stirred at 50° C. for 3 h. The volatiles are removed in vacuo, and the aqueous phase is neutralized with 2M aqueous HCl, followed by extraction with DCM (3×). The combined organic extracts are dried (NaSO), filtered, and evaporated in vacuo to give the title compound C-1 as a white solid. LC-MS B:t R =0.93 min; [M+H] + =357.51.

[0284] (S)-1-(2-((tert-butoxycarbonyl) (methyl)amino)-N,4-dimethylpentanamido)cyclopropane-1-carboxylic acid (C-2)

[0285] Step 1: A 60% dispersion of NaH in mineral oil (2.52 g, 65.7 mmol) is added to a solution of 1-(Boc-amino)cyclopropanecarboxylic acid (4.5 g, 21.9 mmol) in THF (250 mL) at 0 °C. After stirring the RM for 10 min, MeI (5.51 mL, 87.7 mmol) is added and stirring is continued at RT for 16 h. The reaction is quenched with cold HO, acidified with 2 M aqueous HCl, and then extracted with DCM (3x). The combined organic extracts are dried (NaSO), filtered, and evaporated in vacuo to give 1-((tert-butoxycarbonyl)(methyl)amino)cyclopropane-1-carboxylic acid as a white solid. LC-MS B:t R =0.71 min; [M+H] + =216.39.

[0286] Step 2: K2CO3 (4.33 g, 31.4 mmol) is added to a solution of 1-((tert-butoxycarbonyl)(methyl)amino)cyclopropane-1-carboxylic acid (4.5 g, 20.9 mmol) and benzyl bromide (2.79 mL, 23.0 mmol) in MeCN (100 mL) at RT, and the resulting mixture is stirred for 16 h. The RM is concentrated, the residue is partitioned between water and DCM, and the layers are separated. The aqueous phase is re-extracted with DCM (2x), and the combined organic extracts are washed with brine, dried (Na2SO4), filtered, and evaporated. Purification by FC (eluting with 5%-12% EtOAc in hept) gives benzyl 1-((tert-butoxycarbonyl)(methyl)amino)cyclopropane-1-carboxylate as a colorless oil. LC-MS B:t R = 1.02 min; [M+H] + =306.06.

[0287] Step 3: 4M HCl in dioxane (36.0 mL, 144 mmol) is added to a solution of benzyl 1-((tert-butoxycarbonyl)(methyl)amino)cyclopropane-1-carboxylate (4.4 g, 14.4 mmol) in dioxane (60 mL) at RT, and the resulting mixture is heated to 50° C. for 2 h. The RM is concentrated, and the residue is triturated with isopropyl acetate, filtered, and dried under HV to give benzyl 1-(methylamino)cyclopropane-1-carboxylate hydrochloride as a white solid. LC-MS B:t R =0.54 min; [M+H] + =206.27.

[0288] Step 4: HATU (5.06 g, 13.3 mmol) is added portionwise to a solution of Boc-N-methyl-L-leucine (5.0 g, 20.3 mmol), benzyl 1-(methylamino)cyclopropane-1-carboxylate hydrochloride (3.22 g, 13.3 mmol) and DIPEA (9.1 mL, 53.2 mmol) in MeCN (50 mL) at RT, and the resulting mixture is stirred for 16 h. The RM is concentrated, the residue is partitioned between water and DCM, and the layers are separated. The aqueous phase is re-extracted with DCM (2x), and the combined organic extracts are washed with brine, dried (Na2SO4), filtered, and evaporated. Purification by FC (eluting with 15%-24% EtOAc in hept) affords benzyl (S)-1-(2-(tert-butoxy)-2-methyl-1H-pyrazolinone. (N,4-dimethylpentanamido)cyclopropane-1-carboxylate is obtained as an orange oil. LC-MS B:t R = 1.12 min; [M+H] + =433.15.

[0289] Step 5: A solution of benzyl (S)-1-(2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)cyclopropane-1-carboxylate (14.2 g, 34.2 mmol) in EtOH (50 mL) is purged with N2 / vacuum (3x) before adding 10% Pd / C (603 mg, 0.57 mmol). After three additional inactivations, a H2 balloon is attached and the RM is stirred for 3 h. The mixture is concentrated and filtered through a plug of Celite, rinsing with EtOH. The filtrate is concentrated to give the title compound C-2 as a colorless oil. LC-MS B:t R =0.88min;[M+H] + =343.26.

[0290] N-(N-(tert-butoxycarbonyl)-N-methyl-L-leucyl)-N-methyl-D-alanine (C-3) The title compound is prepared from N-(tert-butoxycarbonyl)-N-methyl-D-alanine and Boc-N-methyl-L-leucine following the two-step sequence described for C-1. LC-MS B:t R =0.88min;[M+H] + =331.33. 1 H NMR (400MHz, DMSO) δ 5.03-4.46(m, 2H), 2.95-2.83(m, 2H), 2.75-2.54(m, 4H), 1.58-1.44(m, 2H) ), 1.41(s, 10H), 1.31-1.23(m, 3H), 1.23-1.17(m, 1H), 0.94-0.84(m, 6H).

[0291] 2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-4,4,4-trifluorobutanoic acid (C-4)

[0292] Step 1: NaOAc (12.78 g, 0.156 mol), TFA (2.41 mL, 31.1 mmol) and benzaldehyde (3.34 mL, 32.7 mmol) were added to methyl A solution of 2-amino-4,4,4-trifluorobutanoate hydrochloride (6.81 g, 31.1 mmol) in MeOH (20 mL) was added at room temperature, and the resulting mixture was stirred for 1 h. NaBHCN (2.27 g, 34.3 mmol) was then added, and stirring was continued for 45 min. The mixture was evaporated to dryness, then partitioned between H2O and DCM, and the layers were separated. The aqueous layer was extracted with DCM, and the combined organic extracts were dried (Na2SO4), filtered, and evaporated to give methyl-2-(benzylamino)-4,4,4-trifluorobutanoate as a brown oil, which was used directly in the next step. LC-MS I:t R =0.96 min; [M+H] + =262.37.

[0293] Step 2: NaOAc (12.67 g, 155 mmol), TFA (2.39 mL, 30.9 mmol), and formaldehyde (37% in HO, 2.53 mL, 34 mmol) were added to a solution of methyl-2-(benzylamino)-4,4,4-trifluorobutanoate (8.07 g, 30.9 mmol) in MeOH (100 mL) at RT, and the resulting mixture was stirred at RT for 1 h. NaBHCN (2.25 g, 34.0 mmol) was then added, and stirring was continued. After 1.5 h, formaldehyde (37% in HO, 0.46 mL, 6.18 mmol) and NaBHCN (409 mg, 6.18 mmol) were added, and the mixture was stirred at RT for an additional 2 h. The mixture was evaporated to dryness, partitioned between HO and DCM, and the layers were separated. The aqueous layer is re-extracted with DCM and the combined organic extracts are dried (Na2SO4), filtered and evaporated to give methyl-2-(benzyl(methyl)amino)-4,4,4-trifluorobutanoate as a brown oil which is used directly in the next step. LC-MS I:t R = 1.13 min; [M+H] + =276.45.

[0294] Step 3: A solution of methyl-2-(benzyl(methyl)amino)-4,4,4-trifluorobutanoate (6.48 g, 23.5 mmol) in EtOH (200 mL) is evacuated / purged with Ar (3x) before adding Pd / C (1.25 g, 5 mol%). The RM is evacuated / purged with H2 (3x) and stirred under an H2 atmosphere for 2.5 h. The mixture is filtered and rinsed with MeOH. 4 M HCl (5.89 mL, 23.5 mmol) is added and the mixture is evaporated to dryness to give methyl-4,4,4-trifluoro-2-(methylamino)butanoate hydrochloride as an off-white solid, which is used directly in the next step. LC-MS I:t R =0.60min;[M+H] + =186.37.

[0295] Step 4: HATU (11.26 g, 29.6 mmol) is added portionwise to a solution of Boc-N-methyl-L-leucine (6.24 g, 24.7 mmol), methyl-4,4,4-trifluoro-2-(methylamino)butanoate hydrochloride (5.47 g, 24.7 mmol), and DIPEA (16.9 mL, 98.7 mmol) in DMF (80 mL) at RT, and the resulting mixture is stirred for 1 h. Water is added, and the mixture is extracted with EtOAc (3x). The combined organic extracts are washed successively with saturated aqueous NaHCO3, HO, and brine, dried (Na2SO4), filtered, and concentrated. Purification by FC (eluting with 15% EtOAc in hept) gives methyl-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-4,4,4-trifluorobutanoate as a yellow oil. LC-MS B:t R = 1.06 min; [M+H] + =413.29.

[0296] Step 5: 2M aqueous NaOH (6.9 mL, 13.8 mmol) is added to a solution of methyl-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-4,4,4-trifluorobutanoate (2.84 g, 6.88 mmol) in MeOH (10 mL) at RT, and the mixture is stirred at RT for 1.5 h. The volatiles are removed in vacuo, and the aqueous residue is neutralized with 2M aqueous HCl, then extracted with DCM (3x). The combined organic layers are dried (Na2SO4), filtered, and evaporated to give the title compound C-4 as a white solid. LC-MS B:t R =0.96 min; [M+H] + =399.29.

[0297] 2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-4,4-difluorobutanoic acid (C-5)

[0298] Step 1: A 60% dispersion of NaH in mineral oil (982 mg, 25.6 mmol) is added to a solution of rac-2-((tert-butoxycarbonyl)amino)-4,4-difluorobutanoic acid (3.15 g, 12.5 mmol) and MeI (1.61 mL, 25.6 mmol) in DMF (20 mL) at RT, and the RM is stirred for 1 h. The RM is partitioned between water and EtOAc, and the layers are separated. The aqueous phase is re-extracted with EtOAc (2x), and the combined organic extracts are washed with brine, dried (Na2SO4), filtered, and evaporated to give rac-methyl (R)-2-((tert-butoxycarbonyl)(methyl)amino)-4,4-difluorobutanoate as a yellow oil. LC-MS B:t R =0.88min;[M+H] + =268.19.

[0299] Step 2: TFA (10.0 mL, 131 mmol) is added to a solution of rac-methyl (R)-2-((tert-butoxycarbonyl)(methyl)amino)-4,4-difluorobutanoate (3.34 g, 12.5 mmol) in DCM (20 mL) at RT and the RM is stirred for 1 h. The volatiles are removed in vacuo and the residue is co-evaporated with DCM (3x) to give rac-methyl (R)-4,4-difluoro-2-(methylamino)butanoate 2,2,2-trifluoroacetate, which is used directly in the next step. LC-MS B:t R =0.28min;[M+H] + =168.02.

[0300] Steps 3 and 4: The title compound is prepared from rac-methyl (R)-4,4-difluoro-2-(methylamino)butanoate 2,2,2-trifluoroacetate and Boc-N-methyl-L-leucine following the two-step sequence described for C-1. LC-MS B:t R =0.91 min; [M+H] + =381.13.

[0301] (R)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-3-(3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)propanoic acid (C-6)

[0302] Step 1: Benzyl bromide (4.71 mL, 38.8 mmol) is added to a solution of Boc-D-Asp-OMe (10.00 g, 38.8 mmol) and DIPEA (26.6 mL, 155 mmol) in DMF (71 mL) at RT, and the RM is heated to 50 °C for 2 h. The RM is cooled to RT, then water and EtO are added and the layers are separated. The aqueous layer is extracted with EtO (1x). The combined organic layers are washed with brine, dried (MgSO), filtered, and concentrated. Purification by FC (eluting with 0% to 30% EtOAc in hept) gives 4-benzyl 1-methyl (tert-butoxycarbonyl)-D-aspartate as a colorless oil. LC-MS B:t R=0.97 min; [M+H] + =337.96.

[0303] Step 2: 4M HCl in dioxane (57.9 mL, 240 mmol) is added to a solution of 4-benzyl 1-methyl (tert-butoxycarbonyl)-D-aspartate (8.19 g, 24 mmol) in dioxane (42.3 mL) at RT, and the RM is heated to 50° C. for 30 min. The mixture is cooled to RT and then concentrated to give 4-benzyl 1-methyl D-aspartate.HCl as a yellow solid, which is used directly in the next step. LC-MS B:t R =0.53 min; [M+H] + =238.30.

[0304] Step 3: HATU (10.38 g, 26.5 mmol) was added to a solution of 4-benzyl 1-methyl D-aspartate.HCl (6.84 g, 22.1 mmol), boc-N-methyl-L-leucine (5.58 g, 22.1 mmol), and DIPEA (19.9 mL, 110 mmol) in MeCN (83 mL) at RT, and the resulting mixture was stirred for 10 min. The RM was concentrated, the residue was partitioned between water and DCM, and the layers were separated. The aqueous phase was re-extracted with DCM (2x), and the combined organic extracts were washed with brine, dried (Na2SO4), filtered, and evaporated. Purification by FC (eluting with 0% to 40% EtOAc in hept) gave 4-benzyl 1-methyl N-(tert-butoxycarbonyl)-N-methyl-L-leucyl-D-aspartate as a yellow oil. LC-MS B:t R = 1.10 min; [M+H] + =465.03.

[0305] Step 4: A 60% dispersion of NaH in mineral oil (1.02 g, 26.5 mmol) is added to a solution of 4-benzyl 1-methyl N-(tert-butoxycarbonyl)-N-methyl-L-leucyl-D-aspartate (4.53 g, 8.83 mmol) and MeI (2.22 mL, 35.3 mmol) in DMF (73 mL) at −20° C. The resulting mixture is stirred at −20° C. for 15 min, then quenched with 1 M aqueous HCl (224 mL) and diluted with isopropyl acetate. The layers are separated, and the aqueous layer is extracted with isopropyl acetate (1×). The combined organic extracts are dried (MgSO4), filtered, and concentrated. Purification by FC (eluting with 10% to 40% EtOAc in hept) gives 4-benzyl 1-methyl N-(N-(tert-butoxycarbonyl)-N-methyl-L-leucyl)-N-methyl-D-aspartate as a yellow oil. LC-MS B:t R = 1.11 min; [M+H] + =479.16.

[0306] Step 5: Pd / C (10%, 387 mg, 0.364 mmol) was reacted with 4-benzyl To a solution of 1-methyl N-(N-(tert-butoxycarbonyl)-N-methyl-L-leucyl)-N-methyl-D-aspartate (3.89 g, 7.28 mmol) in MeOH (34 mL) at RT is added and the RM is stirred under an atmosphere of H at RT for 1 h. The RM is filtered and concentrated to give (R)-3-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-4-methoxy-4-oxobutanoic acid as a colorless oil, which is used directly in the next step. LC-MS B:t R =0.89 min; [M+H] + =389.33.

[0307] Step 6: PyBOP (9.86 g, 18.5 mmol) is added to a solution of (R)-3-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-4-methoxy-4-oxobutanoic acid (6.0 g, 15.4 mmol), 2,2,2-trifluoro-N'-hydroxyethanimidamide (3.12 g, 23.2 mmol) and DIPEA (7.93 mL, 46.3 mmol) in DCM (60 mL) at RT and the RM is stirred for 10 min. The RM is concentrated and the residue is partitioned between water and EtOAc and the layers are separated. The aqueous phase is re-extracted with EtOAc (2x) and the combined organic extracts are washed with saturated aqueous NaHCO3, dried (Na2SO4), filtered and evaporated to give an intermediate which is redissolved in dioxane (120 mL) and heated to 100 °C for 2 d. The RM is cooled to RT and concentrated before purification by FC (eluting with 30% EtOAc in hept) to give methyl (R)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-3-(3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)propanoate as a yellow oil. LC-MS B:t R = 1.11 min; [M+H] + =581.08.

[0308] Step 7: 2M aqueous LiOH (34.5 mL, 69.1 mmol) is added to a solution of methyl (R)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-3-(3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)propanoate (6.64 g, 13.8 mmol) in MeOH (200 mL) at RT and the RM is stirred for 15 min. The RM is diluted with DCM (50 mL) and acidified with 2M aqueous HCl. The layers are separated and the aqueous layer is extracted with DCM. The combined organic extracts are washed with brine, dried (MgSO), filtered and concentrated to give the title compound C-6 as a white solid. LC-MS B:t R = 1.02 min; [M+H] + =467.11.

[0309] (R)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-5,5,5-trifluoropentanoic acid (C-7)

[0310] Step 1: Under argon, (R)-2-amino-5,5,5-trifluoropentanoic acid (1514 mg, 8.85 mmol) is suspended in a mixture of a solution of K2CO3 (3231 mg, 23.4 mmol) in HO (30 mL) and THF (25 mL). The solution / suspension is cooled to 0 °C, and a solution of Boc2O (2.69 mL, 11.7 mmol) in THF (5 mL) is added dropwise. The RM is warmed to RT and stirred for 16 h. The RM is diluted with water and extracted once with Et2O (discarded). The pH of the aqueous layer is adjusted to 4 by the addition of solid citric acid, then extracted with DCM (3x). The combined organic extracts are washed with brine, dried over MgSO4, filtered, and concentrated under reduced pressure. The residue is dissolved in MeCN, washed with heptane, and then concentrated to dryness to give 1.36 g of (R)-2-((tert-butoxycarbonyl)amino)-5,5,5-trifluoropentanoic acid as a white solid; LC-MS B:t R =0.79 min; [M+H] + =272.18; 1 H NMR (400MHz, DMSO) δ:12.28-13.12(m, 1H), 7.25(d, J=8.2Hz, 1H), 3.92-4.09(m, 1H), 2.15-2.46(m, 2H), 1.73-1.94(m, 2H), 1.36-1.43(m, 9H).

[0311] Step 2: A 60% dispersion of NaH in mineral oil (285 mg, 11.5 mmol) is added portionwise under argon to an ice-cold solution of (R)-2-((tert-butoxycarbonyl)amino)-5,5,5-trifluoropentanoic acid (1360 mg, 5.01 mmol) and MeI (0.782 mL, 12.5 mmol) in DMF (32 mL). The RM is stirred and allowed to warm from 0 °C up to RT for 4 h. At this stage, the RM is recooled to 0 °C and additional MeI (0.344 mL, 5.52 mmol) and NaH (60.2 mg, 2.51 mmol) are added; the resulting RM is stirred at 0 °C for 1 h to complete the conversion. The mixture is quenched with saturated aqueous NH4Cl and extracted with EtOAc (3x). The combined organic extracts are washed with water and brine, dried over MgSO, filtered and evaporated in vacuo. The crude is purified by FC (0 to 100% EtO in petroleum ether) to give 1.21 g of methyl (R)-2-((tert-butoxycarbonyl)(methyl)amino)-5,5,5-trifluoropentanoate as a colorless oil; LC-MS B:t R =0.97 min; [M+H] + =300.24.

[0312] Step 3: TFA (3 mL, 39.2 mmol) is added to a solution of methyl (R)-2-((tert-butoxycarbonyl)(methyl)amino)-5,5,5-trifluoropentanoate (1208 mg, 4.04 mmol) in DCM (6 mL). The RM is stirred at RT for 1 h to complete the reaction. The mixture is diluted with DCM and the volatiles are removed in vacuo; the residue is co-evaporated with DCM (3x) to give 1.21 g of (R)-5,5,5-trifluoro-1-methoxy-N-methyl-1-oxopentan-2-aminium 2,2,2-trifluoroacetate as a colorless oil, which is used directly in the next step. LC-MS B:t R =0.40min;[M+H] + =200.31.

[0313] Step 4: HATU (1615 mg, 4.25 mmol) is added to a solution of (R)-5,5,5-trifluoro-1-methoxy-N-methyl-1-oxopentan-2-aminium 2,2,2-trifluoroacetate (1209 mg, 3.86 mmol), Boc-N-methyl-L-leucine (1074 mg, 4.25 mmol), and DIPEA (2.64 mL, 15.4 mmol) in DMF (12 mL) at RT under argon. The RM is stirred at RT for 1 h to complete the reaction. The mixture is partitioned between water and EtO. The layers are separated, and the aqueous phase is further extracted with EtO (2x). The combined organic extracts are washed with water and brine, dried over MgSO, filtered, and evaporated in vacuo. The crude product is purified by FC (0-80% EtOAc in heptane, monitored by ELSD) to give 1.51 g of methyl (R)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-5,5,5-trifluoropentanoate as a colorless oil. LC-MS B:t R = 1.11 min; [M+H] + =427.36.

[0314] Step 5: NaOH 1M (7 mL, 7 mmol) is added to a solution of methyl (R)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-5,5,5-trifluoropentanoate (1505 mg, 3.52 mmol) in dioxane (14 mL) at RT. The RM is stirred at RT for 1 h to complete the reaction. The RM is neutralized by the addition of NH4Cl, and then the dioxane is removed under reduced pressure. The remaining aqueous suspension is acidified with citric acid (pH around 3) and then extracted with DCM (3x). The combined organic layers are dried over MgSO4 and concentrated under reduced pressure to give 1.48 g of the title compound C-7 as a colorless oil that foams under vacuum. LC-MS B:t R =0.99 min; [M+H] + =413.35.

[0315] (R)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-5,5-difluorohexanoic acid (C-8)

[0316] Step 1: A 60% dispersion of NaH in mineral oil (224 mg, 5.61 mmol) is added to a mixture of (R)-2-(tert-butoxycarbonylamino)-5,5-difluorohexanoic acid (695 mg, 2.55 mmol) and MeI (0.398 mL, 6.37 mmol) in DMF (12 mL) at 0 °C under argon. The RM is stirred at rt for 6 h. Excess MeI (0.0795 mL, 1.27 mmol) and NaH (51 mg, 1.27 mmol) are added at 0 °C, and the mixture is further stirred for 1 h to ensure complete conversion to the bis-alkylated product. The mixture is quenched with saturated aqueous NH4Cl and diluted with EtOAc. The layers are separated, and the aqueous layer is further extracted with EtOAc (2x). The combined organic extracts were washed successively with saturated aqueous NaSO, water, and brine, dried over MgSO, filtered, and evaporated in vacuo to give 986 mg of crude methyl (R)-2-((tert-butoxycarbonyl)(methyl)amino)-5,5-difluorohexanoate as a pale yellow oil, which was used directly in the next step. LC-MS B:t R =0.95min;[M+H] + =296.32.

[0317] Step 2: TFA (2 mL, 26.1 mmol) is added to a solution of methyl (R)-2-((tert-butoxycarbonyl)(methyl)amino)-5,5-difluorohexanoate (836 mg, 2.15 mmol) in DCM (4 mL). The RM is stirred at room temperature for 4 h to bring the reaction to near completion. The mixture is diluted with DCM and the volatiles are removed in vacuo; the residue is co-evaporated with DCM (3x) to give 665 mg of (R)-5,5-difluoro-1-methoxy-N-methyl-1-oxohexan-2-aminium 2,2,2-trifluoroacetate as a pale yellow solid, which is used directly in the next step. LC-MS B:t R =0.42 min; [M+H] +=196.37.

[0318] Step 3: HATU (899 mg, 2.37 mmol) was treated with (R)-5,5-difluoro-1-methoxy-N-methyl-1-oxohexan-2-aminium at RT under N. To a mixture of 2,2,2-trifluoroacetate (665 mg, 2.15 mmol), Boc-N-methyl-L-leucine (598 mg, 2.37 mmol), and DIPEA (1.84 mL, 10.8 mmol) in DMF (10 mL) is added. The RM is stirred at RT for 1 h to complete the reaction. The mixture is partitioned between water and EtOAc. The layers are separated, and the aqueous layer is further extracted with EtOAc (2x). The combined organic extracts are washed with water and brine, dried over MgSO4, filtered, and evaporated in vacuo. The crude product is purified by FC (0-50% EtOAc in heptane, monitored by ELSD) to give 908 mg of methyl (R)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-5,5-difluorohexanoate as a colorless oil. LC-MS B:t R = 1.08 min; [M+H] + =423.35.

[0319] Step 4: NaOH 1M (4.3 mL, 4.3 mmol) is added at RT to a solution of methyl (R)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-5,5-difluorohexanoate (1069 mg, 2.53 mmol) in dioxane (8.6 mL). The RM is stirred at RT for 30 min to complete the reaction. The RM is neutralized by the addition of NH4Cl, and the solvent is then removed under reduced pressure. The remaining aqueous suspension is acidified with citric acid (pH around 3) and then exhaustively extracted with DCM (3x). The combined organic layers are dried over MgSO4 and concentrated under reduced pressure to give, after exhaustive evaporation, 1.03 g of the title compound C-8 as a white foam. LC-MS B:t R =0.98 min; [M+H] + =409.43.

[0320] (RS)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-4,4-difluoropentanoic acid (C-9)

[0321] Step 1: Under argon, methyl 2-amino-4,4-difluoropentanoate hydrochloride (1000 mg, 4.67 mmol) is dissolved in a mixture of THF (10 mL) and HO (10 mL). NaHCO (1960 mg, 23.3 mmol) is added, followed by BocO (1122 mg, 5.14 mmol). The mixture is stirred at RT for 1 h to complete the reaction. The RM is diluted with water and exhaustively extracted with EtOAc (4x). The combined organic extracts are washed with brine, dried over MgSO, filtered, and concentrated under reduced pressure to give 1.40 g of crude rac-methyl (R)-2-((tert-butoxycarbonyl)amino)-4,4-difluoropentanoate as a light brown oil; LC-MS B:t R =0.86 min; [M+H] + =268.26; 1 H NMR (400MHz, DMSO) δ 7.41(d, J=8.2Hz, 1H), 4.21(m, 1H), 3.64(s, 3H), 2.45-2.17(m, 2H), 1.62(t, J=19.2Hz, 3H), 1.38(s, 9H).

[0322] Step 2: A 60% dispersion of NaH in mineral oil (221 mg, 5.76 mmol) is added portionwise under argon to a solution of crude rac-methyl (R)-2-((tert-butoxycarbonyl)amino)-4,4-difluoropentanoate (1400 mg, 5.24 mmol) and MeI (0.362 mL, 5.76 mmol) in DMF (10 mL) at RT. The RM is stirred at RT for 1 h to complete the reaction: the mixture is quenched with saturated aqueous NH4Cl and extracted with EtOAc (3x). The combined organic extracts are washed with water and brine, dried over Na2SO4, filtered, and evaporated under vacuum to give m = 1.346 g of crude rac-methyl (R)-2-((tert-butoxycarbonyl)(methyl)amino)-4,4-difluoropentanoate as a yellowish oil. The crude product is used directly in the next step without purification; LC-MS B:t R =0.93 min; [M+H] + =282.25.

[0323] Step 3: TFA (3.74 mL, 47.8 mmol) is added to a solution of crude rac-methyl (R)-2-((tert-butoxycarbonyl)(methyl)amino)-4,4-difluoropentanoate methyl (R)-2-((tert-butoxycarbonyl)(methyl)amino)-5,5,5-trifluoropentanoate (1346 mg, 4.78 mmol) in DCM (10 mL). The RM is stirred at RT for 6 h to complete the reaction. The mixture is diluted with DCM and the volatiles are removed in vacuo; the residue is co-evaporated with DCM (3x) to give 1.83 g of rac-methyl (R)-4,4-difluoro-2-(methylamino)pentanoate 2,2,2-trifluoroacetate as a pale orange oil, which is used directly in the next step. LC-MS B:t R =0.32~34min; [M+H] + =182.31.

[0324] Step 4: HATU (2593 mg, 6.82 mmol) is added to a solution of rac-methyl (R)-4,4-difluoro-2-(methylamino)pentanoate 2,2,2-trifluoroacetate (1830 mg, 6.2 mmol), Boc-N-methyl-L-leucine (1568 mg, 6.2 mmol), and DIPEA (3.18 mL, 18.6 mmol) in DMF (20 mL) at RT under argon. The RM is stirred at RT for 1 h to complete the reaction. The mixture is partitioned between HO and EtOAc. The layers are separated, and the aqueous phase is further extracted with EtOAc (2x). The combined organic extracts are washed with water and brine, dried over NaSO, filtered, and evaporated in vacuo. The crude product is purified by FC (50% to 100% EtOAc in heptane, monitored by ELSD) to give 1.66 g of methyl (RS)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-4,4-difluoropentanoate as a pale yellow oil. LC-MS B:t R = 1.06 min; [M+H] + =409.33.

[0325] Step 5: NaOH 1M (8.1 mL, 8.1 mmol) was added to methyl (R A solution of S)-2-((S)-2-((tert-butoxycarbonyl)(methyl)amino)-N,4-dimethylpentanamido)-4,4-difluoropentanoate (1660 mg, 4.06 mmol) in dioxane (20 mL) is added. The RM is stirred at 60 °C for 1 h to allow complete conversion. The RM is neutralized by the addition of NH4Cl, and then the dioxane is removed under reduced pressure. The remaining aqueous suspension is acidified (pH around 3) by dropwise addition of 2N aqueous HCl, and then extracted with DCM (3x). The combined organic layers are dried over MgSO4 and concentrated under reduced pressure to give 1.60 g of the title compound C-9 as a light yellow oil. LC-MS B:t R =0.95min;[M+H] + =395.29.

[0326] Synthesis of Compounds of Formula (I) General method: GM-A

[0327] Example 1: (3S,7S,10R,13R)-13-benzyl-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide

[0328] Step 1: HATU (1.03 g, 2.58 mmol) is added to a solution of B-1 (1.24 g, 2.46 mmol), C-4 (980 mg, 2.46 mmol), and DIPEA (1.26 mL, 7.38 mmol) in DMF (20 mL) at RT, and the RM is stirred for 30 min. The RM is partitioned between water and EtOAc, and the layers are separated. The aqueous phase is re-extracted with EtOAc (2x), and the combined organic extracts are washed with brine, dried (Na2SO4), filtered, and evaporated. The crude product is purified by prep. HPLC (basic) to give benzyl 6-(((6S,9R,12R)-12-benzyl-6-isobutyl-2,2,5,8-tetramethyl-4,7,10-trioxo-9-(2,2,2-trifluoroethyl)-3-oxa-5,8,11-triazatridecan-13-yl)oxy)-3-fluoroquinoline-5-carboxylate as a white solid. LC-MS I:t R = 1.45 min; [M+H] + =811.73. Note: The second stereoisomer, benzyl 6-(((6S,9S,12R)-12-benzyl-6-isobutyl-2,2,5,8-tetramethyl-4,7,10-trioxo-9-(2,2,2-trifluoroethyl)-3-oxa-5,8,11-triazatridecan-13-yl)oxy)-3-fluoroquinoline-5-carboxylate, is also isolated as a white solid. LC-MS I:t R = 1.43 min; [M+H] + =811.66.

[0329] Step 2: A solution of benzyl 6-(((6S,9R,12R)-12-benzyl-6-isobutyl-2,2,5,8-tetramethyl-4,7,10-trioxo-9-(2,2,2-trifluoroethyl)-3-oxa-5,8,11-triazatridecan-13-yl)oxy)-3-fluoroquinoline-5-carboxylate (519 mg, 0.61 mmol) in EtOH (10 mL) is evacuated / purged with N (3x) before adding 10% Pd / C (32 mg, 5 mol%). The RM is evacuated / purged with H (3x) and stirred under an atmosphere of H for 2 h. The RM is filtered through a pad of Celite and the filtrate is concentrated in vacuo to give 6-(((6S,9R,12R)-12-benzyl-6-isobutyl-2,2,5,8-tetramethyl-4,7,10-trioxo-9-(2,2,2-trifluoroethyl)-3-oxa-5,8,11-triazatridecan-13-yl)oxy)-3-fluoroquinoline-5-carboxylic acid as a white solid. LC-MS I:t R =0.73 min; [M+H] + =721.58.

[0330] Step 3: HATU (134 mg, 0.35 mmol) was added to 6-(((6S,9R,12 A solution of R)-12-benzyl-6-isobutyl-2,2,5,8-tetramethyl-4,7,10-trioxo-9-(2,2,2-trifluoroethyl)-3-oxa-5,8,11-triazatridecan-13-yl)oxy)-3-fluoroquinoline-5-carboxylic acid (244 mg, 0.34 mmol), A-1 (113 mg, 0.34 mmol) and DIPEA (0.18 mL, 1.0 mmol) in DMF (5 mL) was added at RT and the RM was stirred for 1 h. The RM is then directly purified by prep. HPLC (basic) to give tert-butyl (S)-3-(6-(((6S,9R,12R)-12-benzyl-6-isobutyl-2,2,5,8-tetramethyl-4,7,10-trioxo-9-(2,2,2-trifluoroethyl)-3-oxa-5,8,11-triazatridecan-13-yl)oxy)-3-fluoroquinoline-5-carboxamido)-4-((2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)amino)-4-oxobutanoate as a white solid. LC-MS I:t R = 1.33 min; [M+H] + =1017.02.

[0331] Step 4: TFA (3.3 mL, 42.8 mmol) is added to a solution of tert-butyl (S)-3-(6-(((6S,9R,12R)-12-benzyl-6-isobutyl-2,2,5,8-tetramethyl-4,7,10-trioxo-9-(2,2,2-trifluoroethyl)-3-oxa-5,8,11-triazatridecan-13-yl)oxy)-3-fluoroquinoline-5-carboxamido)-4-((2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)amino)-4-oxobutanoate (351 mg, 0.27 mmol) in DCM (9 mL) at RT and the RM is stirred for 3 h. The RM is concentrated in vacuo and the residue is redissolved in DCM and concentrated in vacuo again (2x). The residue is dissolved in DMF (4 mL), then DIPEA (0.37 mL, 2.2 mmol) and HATU (123 mg, 0.32 mmol) are added and the RM is stirred for 1 h. The RM is partitioned between water and EtOAc and the layers are separated. The aqueous phase is re-extracted with EtOAc (2x) and the combined organic extracts are washed with brine, dried (Na2SO4), filtered and evaporated. The crude product is purified by prep. HPLC (basic) to give the title compound as a white solid. LC-MS I:t R = 1.06 min; [M+H] + =842.65.

[0332] Table MC-1 below lists compounds of general formula (I) prepared from the corresponding building blocks A, B, and C in the same manner as described in General Method A (GM-A) above. * indicates example compounds isolated during synthesis, in most cases as minor epimers due to epimerization of chiral centers, and isolated by prep. HPLC purification of the final synthetic step. In certain cases, enantiomerically or diastereomerically pure components undergo epimerization during synthesis, and example compounds are isolated as mixtures of epimers.

[0333] [Table 4]

[0334] [Table 5]

[0335] The following table of examples lists example compounds of formula (I) that were prepared according to the methods described above.

[0336] [Table 6]

[0337] [Table 7]

[0338] [Table 8]

[0339] [Table 9]

[0340] [Table 10]

[0341] [Table 11]

[0342] [Table 12]

[0343] Table 3: Structures of the compounds of Examples 1 to 32

[0344] [Table 13]

[0345] Table 4: Structures of the compounds of Examples 33-58

[0346] [Table 14]

[0347] II. Biological Testing The compounds of the present invention may be further characterized for their general pharmacokinetic and pharmacological properties, for example, for their bioavailability in different species (such as rats or dogs), using conventional assays well known in the art; or for their properties with respect to drug safety and / or toxicological properties, for example, cytochrome P450 enzyme inhibition and time-dependent inhibition, pregnane X receptor (PXR) activation, glutathione binding, or phototoxic behavior, using conventional assays well known in the art.

[0348] Biological in vitro assays EC of the compound 50 and E max Value evaluation The corrector activity of compounds of formula (I) for CFTR is determined according to the following experimental method, which measures the effect of overnight incubation with compounds on F508del-CFTR cell surface expression in a recombinant U2OS cell line (DiscoveRx, #93-0987C3), which contains (i) human F508del-CFTR tagged with Prolink (PK = short β-galactosidase fragment) and (ii) It is designed to simultaneously express the remaining portion of the β-galactosidase enzyme (Enzyme Acceptor; EA) localized to the plasma membrane. Upon incubation with a compound that increases PK-tagged F508del-CFTR in the plasma membrane, the EA fragment is complemented to form functional β-galactosidase enzyme, which is quantified by a chemiluminescent reaction.

[0349] Briefly, cells are seeded at 3500 cells / well in 20 μl of full medium (McCoy's 5a (#36600-021, Gibco) + 10% FBS Gibco + penicillin / streptomycin) in 384-well low-volume plates (Corning, #3826). After incubating the cells for 5 h in an incubator, 5 μl / well of a compound dilution series (5x working stock in complete medium) is added. The final DMSO concentration in the test is 0.25%. Cells are incubated with compounds for 16 h in an incubator at 37°C and 5% CO2. The next day, cell plates are incubated in the dark at RT for 2 h. Then, 10 μl / well of Flash detection reagent (DiscoverX, #93-0247) is added, and the plates are incubated for an additional 30 min at RT in the dark, and chemiluminescence is measured. Concentration-response curves were constructed using the compound-specific maximum effect as the upper plateau, and compound-specific EC 50 Determine the E value of the corrector lumacaftor max Compound-specific E max Calculate the value (E max Lumacaftor = 100%).

[0350] EC to be calculated 50 Values ​​may vary with each day's assay run. This type of variation is known to those skilled in the art. EC values ​​obtained from several measurements 50 The values ​​are reported as geometric mean values. max Values ​​may vary with each day's assay run. This type of variation is known to those skilled in the art. E obtained from several measurements max Values ​​are reported as arithmetic means.

[0351] [Table 15]

[0352] Evaluation of the inhibition of midazolam hydroxylation by the enzyme CYP450 3A4 by compounds of formula (I) CYP3A4 inhibition was tested using midazolam 1'-hydroxylation as a marker reaction. Midazolam was used at a single concentration of 5 μM, near its Km (Michaelis-Menten constant), and test compounds were incubated at eight different concentrations up to 50 μM. Nicardipine was used as a positive control. Microsomal incubations were performed to assess the NADPH-ligand activity. The reaction was initiated by the addition of an NADPH-regenerating system and terminated by the addition of excess ice-cold organic solvent. The formation of 1'-hydroxymidazolam was measured by liquid chromatography-tandem mass spectrometry (LC / MS-MS), and IC values ​​were calculated.

[0353] [Table 16]

[0354] Evaluation of the inhibition of BSEP transporter channels by compounds of formula (I) The inhibitory potential of human BSEP was examined in vesicular uptake assays using 0.2 mM taurocholate (TCA, as a mixture of 3H-TCA and cold TCA) as a model substrate. For rat, dog, and monkey Bsep inhibition experiments, the TCA concentrations were 2, 0.2, and 0.2 mM (as a mixture of 3H-TCA and cold TCA), respectively.

[0355] Inverted HEK293-derived membrane vesicles (0.5 mg total protein / mL) expressing BSEP / Bsep were incubated in the presence of 5 mM ATP or AMP. Incubations were carried out at 37°C in vesicular transport buffer (2 mM Hepes-Tris, pH 7.4, 50 mM sucrose, 100 mM KNO, 10 mM Mg(NO)) containing model substrates alone or in combination with various concentrations of test substance (TA) ranging from 0.03 mM to 100 mM. The incubation time was 5 min for human, rat, and monkey BSEP / Bsep and 2 min for canine Bsep. TCA uptake was stopped by the addition of ice-cold wash buffer (10 mM Tris-HCl, pH 7.4, 50 mM sucrose, and 100 mM KNO), and the membrane suspension was filtered through a nitrocellulose membrane filter (0.45 mm pore size) using a rapid filtration system (Millipore, Zug, Switzerland). The membrane suspension retained on the filter was washed twice with ice-cold wash buffer and then transferred to a scintillation vial. After adding 3.5 mL of scintillation cocktail, Filter-Count (Perkin Elmer, Zurich, Switzerland), total radioactivity was counted using a Tri-Carb 2300 TR liquid scintillation analyzer (Packard Bioscience, Zurich, Switzerland). Prior to the experiment, the nitrocellulose filter was saturated with 1 mM TCA. Sucrosporin A (0.03 mM–100 mM) was used as a positive control.

[0356] In the screening assay, each concentration was tested once, but for compounds of interest the assay was repeated and each concentration was tested in triplicate.

[0357] BSEP / Bsep-mediated net uptake rates were calculated as the difference between the uptake rates obtained in the presence of ATP or AMP. Net uptake rates are presented as arithmetic means and standard deviations (SD), where applicable.

[0358] Data from inhibition experiments were evaluated by plotting inhibitor concentration (logarithmic scale) against net uptake rate. IC50 values ​​were then determined from the plot by nonlinear regression using the following equation:

[0359]

number

[0360] where y is the net uptake rate [(pmol / (mg protein min)], x is the inhibitor concentration (mM), and s is the slope at the reversal point. int of inversion), and Top and Bottom are the maximum and minimum values ​​for the net uptake rate. The minimum value (bottom) was constrained to 0. For all graph data evaluations, the GraphPad Prism software package (version 8.1.1, GraphPad Software Inc., La Jolla, USA) was used.

[0361] The calculated IC50 value may vary depending on the assay procedure performed each day. This type of variation is known to those skilled in the art. The IC50 values ​​obtained from several determinations are reported as geometric means.

[0362] [Table 17]

[0363] reference Boyle MP et al. (2014). Lancet Respir Med 2(7):527–538.

[0364] Dalemans W et al. (1991). Nature 354:526-528.

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[0367] Elborn JS(2016) Lancet 388:2519~2531.

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[0370] Am J Physiol Cell Physiol 281(5):C1734~1742.

[0371] Hutt DM (2011) ACS Med Chem Lett 2(9):703~707.

[0372] Keating D (2018) N Engl J Med 379(17):1612~1620.

[0373] The Big D (2021) J Med Chem 64(11):7241~7260.

[0374] Marchesin V (2023).

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[0380] Veit G (2020) JCI Insight 5(18):e139983.

Claims

1. A compound of formula (I) or a pharmaceutically acceptable salt thereof: 【Chemical 1】 (In the formula, X is -CR Ｘ１ R Ｘ２ represents; (R Ｘ１ and R Ｘ２ together with the carbon atoms to which they are attached: - C ３－６ -cycloalkane-1,1-diyl; - C ３－６ -cycloalkane-1,1-diyl, independently containing one C １－３ -substituted by alkoxy, fluoro or hydroxy; or the C substituted by two fluoro ３－６ -cycloalkane-1,1-diyl; - C ４－６ -heterocycloalkane-diyl having one ring nitrogen atom, and if the nitrogen has a free valence, the nitrogen is unsubstituted or substituted by one substituent, and the substituent is C １－４ -Alkyl and -COO-C １－３ -alkyl, ４－６ -heterocycloalkane-diyl; Or, - C ４－６ -heterocycloalkane-diyl having one ring oxygen atom, ４－６ -heterocycloalkane-diyl; or forming a ring which is R Ｘ１ represents hydrogen, and R Ｘ２ teeth, - C １－６ - alkyl; - C １－４ -fluoroalkyl; - C ３－６ -cycloalkyl; - C １－３ - alkyl, and one -- hydroxy; -- C １－４ -alkoxy; Or, -- -L Ｘ２ -Ar Ｘ２ (-L Ｘ２ are independently a direct bond or C １－３ represents alkylene; Ar Ｘ２ independently represent a 5- or 6-membered heteroaryl; Ｘ２ are independently unsubstituted or substituted by 1 or 2 substituents, said substituents being C １－４ -Alkyl, C １－３ -alkoxy, halogen, C ３－６ -cycloalkyl and C １－３ -fluoroalkyl); The C substituted by １－３ - alkyl; ) and R １ is C １－４ - represents alkyl; Or a fragment 【Chemistry 2】 represents a heterocycle in which R ２ is C １－４ represents alkyl; R ３ is C １－６ represents alkyl; R ４ represents a 5-membered heteroaryl, which is independently unsubstituted or substituted with 1, 2, or 3 substituents, and the substituents are selected from the group consisting of C １－４ - alkyl; C １－４ -alkoxy; C １－３ -fluoroalkyl; C １－３ -fluoroalkoxy; halogen; cyano; and C ３－６ -cycloalkyl; Ar １ represents an 8- to 10-membered bicyclic heteroarylene; the 8- to 10-membered bicyclic heteroarylene is independently unsubstituted or substituted by 1 or 2 substituents, the substituents being C １－４ -Alkyl, C １－３ -fluoroalkyl, C １－４ -alkoxy C １－３ - independently selected from fluoroalkoxy, cyano and halogen; [the above group Ar １ In the formula, the -CO- group and oxygen (i.e., Ar １ to the rest of the molecule) is represented by the group Ar １ and Ar ２ teeth, - represents phenyl or 5- to 6-membered heteroaryl, which phenyl or 5- to 6-membered heteroaryl is unsubstituted or substituted by 1 or 2 substituents, and said substituents are selected from the group consisting of C １－４ -Alkyl, C １－３ -fluoroalkyl, halogen, cyano, C ３－６ -cycloalkyl, C １－６ -alkoxy and C １－３ -fluoroalkoxy.

2. Formula (I Ｅ 2. A compound of formula (I) as defined in claim 1, which is also a compound of formula (I) or a pharmaceutically acceptable salt thereof: 【Chemistry 3】

3. X is -CR Ｘ１ R Ｘ２ represents; (R Ｘ１ and R Ｘ２ together with the carbon atoms to which they are attached, ３－６ -cycloalkane-1,1-diyl; or R Ｘ１ represents hydrogen, and R Ｘ２ teeth, - C １－６ - alkyl; - C １－４ -fluoroalkyl; Or, - -L Ｘ２ -Ar Ｘ２ (-L Ｘ２ independently represent methylene; Ar Ｘ２ independently represent a 5-membered heteroaryl; Ｘ２ is independently substituted with one substituent, said substituent being C １－４ -Alkyl, C １－３ -alkoxy, halogen, C ３－６ -cycloalkyl and C １－３ -fluoroalkyl); ) and R １ is C １－４ - represents alkyl; Or a fragment 【Chemistry 4】 represents a heterocycle in which 3. The compound according to claim 1 or 2, or a pharmaceutically acceptable salt thereof.

4. Fragment 【Chemistry 5】 3. The compound of claim 1 or 2, or a pharmaceutically acceptable salt thereof.

5. R ２ The compound according to any one of claims 1 to 4, or a pharmaceutically acceptable salt thereof, wherein: represents methyl.

6. R ３ The compound according to any one of claims 1 to 5, or a pharmaceutically acceptable salt thereof, wherein R represents isobutyl.

7. R ４ represents a 5-membered heteroaryl, the 5-membered heteroaryl being independently substituted with 1 or 2 substituents, the substituents being C １－４ -alkoxy; halogen; and C ３－６ -cycloalkyl; or a pharmaceutically acceptable salt thereof.

8. Ar ２ but, phenyl, which is unsubstituted; or - 6-membered heteroaryl, independently unsubstituted or substituted by 1 or 2 substituents, said substituents being selected from the group consisting of C １－４ -Alkyl, C １－３ -fluoroalkyl, halogen, C ３－６ -cycloalkyl, C １－６ -alkoxy and C １－３ -the 6-membered heteroaryl independently selected from -fluoroalkoxy; The compound according to any one of claims 1 to 7, or a pharmaceutically acceptable salt thereof, which represents:

9. Ar １ is quinoline-diyl substituted by 1 or 2 substituents, said substituents being C １－４ -Alkyl, C １－３ -fluoroalkyl, C １－４ -alkoxy C １－３ 9. The compound of claim 1, wherein R represents quinolin-diyl, independently selected from fluoroalkoxy, cyano, and halogen; or a pharmaceutically acceptable salt thereof.

10. The compound is (3S,7S,10R,13R)-13-benzyl-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-22-((6-methoxypyridin-2-yl)methyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aS,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-22-((6-methoxypyridin-2-yl)methyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-5-fluoro-13-isobutyl-22-((6-methoxypyridin-2-yl)methyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-22-((6-methoxypyridin-2-yl)methyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (3S,7S,10R,13R)-13-benzyl-10-(2,2-difluoroethyl)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecanoic acid Hydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10S,13R)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo -10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazole- 5-yl)ethyl)-12-methyl-7,11,14,20-tetraoxo-22-(pyridin-2-ylmethyl)-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-12-methyl-7,11,14,20-tetraoxo-22-(pyridin-2-ylmethyl)-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,2 3-Hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-12-methyl-7,11,14,20-tetraoxo-22-(pyridin-2-ylmethyl)-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-5-fluoro-13-isobutyl-12-methyl-7,11,14,20-tetraoxo-22-(pyridin-2-ylmethyl)-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-20-fluoro-7-isobutyl-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-13-(pyridin-2-ylmethyl)-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-N-(2-(4-fluoro-3-methoxyisoxazol-5-yl)ethyl)-7-isobutyl-6,9-dimethyl-1,5,8,11-tetraoxo-13-(pyridin-2-ylmethyl)-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9,10-trimethyl-1,5,8,11-tetraoxo-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro- [1] Oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9,10-trimethyl-1,5,8,11-tetraoxo-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3'S,7'S,13'R)-20'-fluoro-7'-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13'-((6-methoxypyridin-2-yl)methyl)-6',9'-dimethyl-1',5',8',11'-tetraoxo-2',3',4',5',6',7',8',9',11',12',13',14'-dodecahydro-1'H-spiro[cyclopropane-1,10'-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline]-3'-carboxamide; (3'S,7'S,13'R)-20'-fluoro-7'-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-13'-((6-methoxypyridin-2-yl)methyl)-6',9'-dimethyl-1',5',8',11'-tetraoxo-2',3',4',5',6',7',8',9',11',12',13',14'-dodecahydro-1'H-spiro[cyclopropane-1,10'-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline]-3'-carboxamide; (3'S,7'S,13'R)-20'-fluoro-N-(2-(4-fluoro-3-methoxyisoxazol-5-yl)ethyl)-7'-isobutyl-13'-((6-methoxypyridin-2-yl)methyl)-6',9'-dimethyl-1',5',8',11'-tetraoxo-2',3',4',5',6',7',8',9',11',12',13',14'-dodecahydro-1'H-spiro[cyclopropane-1,10'-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline]-3'-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-((3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)methyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-((3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)methyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-7-isobutyl-N-(2-(4-methoxy-2H-1,2,3-triazol-2-yl)ethyl)-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-((3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)methyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-20-fluoro-7-isobutyl-13-((6-methoxypyridin-2-yl)methyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-((3-(trifluoromethyl)-1,2,4-oxadiazol-5-yl)methyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; ((3S,7S,10R,13R)-20-fluoro-13-((5-fluoropyridin-2-yl)methyl)-7-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (3S,7S,10R,13R)-20-fluoro-13-((5-fluoropyridin-2-yl)methyl)-7-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-6,9-dimethyl-1,5,8,11-tetraoxo-10-(2,2,2-trifluoroethyl)-1,2,3,4,5,6,7,8,9,10,11,12,13,14-tetradecahydro-[1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-3-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-12-methyl-22-((6-methylpyridin-2-yl)methyl)-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-5-fluoro-13-isobutyl-N-(2-(3-methoxy-1,2,4-oxadiazol-5-yl)ethyl)-12-methyl-22-(oxazol-4-ylmethyl)-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; (9S,13S,19aR,22R)-5-fluoro-22-((5-fluoro-6-methylpyridin-2-yl)methyl)-13-isobutyl-N-(2-(3-methoxyisoxazol-5-yl)ethyl)-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; or (9S,13S,19aR,22R)-N-(2-(5-cyclopropyl-2H-tetrazol-2-yl)ethyl)-5-fluoro-22-((5-fluoro-6-methylpyridin-2-yl)methyl)-13-isobutyl-12-methyl-7,11,14,20-tetraoxo-7,8,9,10,11,12,13,14,17,18,19,19a,20,21,22,23-hexadecahydro-16H-pyrido[2',1':6,7][1]oxa[4,7,10,14]tetraazacycloheptadecyno[16,17-f]quinoline-9-carboxamide; 2. The compound of claim 1, wherein:

11. A compound according to any one of claims 1 to 10 or a pharmaceutically acceptable salt thereof, and at least and at least one therapeutically inactive excipient.

12. A compound according to any one of claims 1 to 10 or a pharmaceutically acceptable salt thereof for use as a pharmaceutical.

13. 11. A compound according to any one of claims 1 to 10, or a pharmaceutically acceptable salt thereof, for use in the treatment of CFTR-related diseases and disorders, including cystic fibrosis.

14. 11. Use of a compound according to any one of claims 1 to 10, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for the treatment of CFTR-related diseases and disorders, including cystic fibrosis.

15. 11. A method for treating CFTR-related diseases and disorders, including cystic fibrosis, comprising administering to a subject in need thereof an effective amount of a compound according to any one of claims 1 to 10, or a pharmaceutically acceptable salt thereof.

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