Soy allergy antigen

Abstract

The present invention provides a novel antigen for soy allergy, a method and kit for diagnosing soy allergy, a pharmaceutical composition containing the antigen, soybeans or soybean products from which the antigen has been removed, and a tester for determining the presence or absence of soybean antigen in a target object. [Solution] The diagnostic kit of the present invention comprises at least one of the proteins identified as (1) to (8), or at least one of the proteins identified as (1α) to (55α).

Description

Technical Field

[0001] The present invention relates to a novel antigen for soybean allergy. The present invention also relates to a diagnostic kit, a diagnostic composition, and a diagnostic method for soybean allergy. The present invention also relates to a pharmaceutical composition containing the antigen, and soybeans or soybean processed products in which the antigen is removed or reduced. The present invention further relates to a tester for determining the presence or absence of soybean antigen in a subject.

Background Art

[0002] In the sera and tissues of allergic patients, IgE antibodies specific to certain antigens are produced. An allergic reaction is induced by the physiological results caused by the interaction between this IgE antibody and a specific antigen.

[0003] In conventional allergy test reagents, it is often the case that antigen reagents are prepared simply by grinding allergen candidate foods, materials, etc. (Patent Document 1). Thus, conventional antigen reagents do not necessarily contain a specific antigen protein (allergen component) that causes an allergic reaction alone, but contain a plurality of protein components. Therefore, in conventional antigen reagents, the content varies for each allergen component. For this reason, among the numerous proteins contained in conventional antigen reagents, only when the protein with a content exceeding the threshold value for which a positive reaction can be determined for binding with an IgE antibody is an allergen component, it is possible to detect a positive reaction in an allergy test. Conversely, for patients in whom an IgE antibody binds to an allergen component with a low content in an allergen such as food, a positive reaction cannot be determined even using conventional allergy test reagents, and the diagnostic efficiency is not sufficiently high.

[0004] Furthermore, the symptoms and severity of allergic reactions do not necessarily correlate with the amount of allergen component present. Even if a patient's IgE antibodies react to trace amounts of allergen components in a potential allergen food or material, allergic symptoms may appear or be related to the severity of the reaction.

[0005] Attempts are underway to improve diagnostic efficiency by examining IgE antibodies against each component that makes up the crude antigen, thereby distinguishing between sensitization directly related to diagnosis and sensitization based on cross-reactivity with pan-allergens, etc. Regarding soy allergens, eight are currently registered with the World Health Organization / International Union for Immunology (WHO / IUIS).

[0006] However, in order to improve the reliability of allergy tests, it is necessary to comprehensively identify allergen components in candidate foods and materials, but the patient detection rate by measuring the above allergen components is still insufficient. Identifying novel soy allergens is extremely important not only for improving the accuracy of diagnostic agents but also as a target for low-allergen foods and therapeutic drugs.

[0007] On the other hand, regarding the separation and purification of proteins, various methods have been investigated to separate and purify proteins and nucleic acids from cell extracts and other sources. Dialysis using salt concentration and centrifugation are examples of such methods.

[0008] Furthermore, numerous purification methods utilizing the differences in charge and molecular weight of protein and nucleic acid residues have been investigated. Examples of purification methods utilizing charge include column chromatography using ion exchange resins and isoelectric focusing. Examples of purification methods utilizing differences in molecular weight include centrifugation, column chromatography using molecular weight sieves, and SDS-PAGE (DED). An example is sodium sil sulfate-polyacrylamide gel electrophoresis.

[0009] In recent years, two-dimensional electrophoresis, which involves isoelectric focusing in the first dimension and SDS-PAGE in the second dimension, has been used as a method for separating and purifying diverse proteins from small sample sizes. The applicants have developed two-dimensional electrophoresis methods with high separation capabilities (Patent Documents 2-5). [Prior art documents] [Patent Documents]

[0010] [Patent Document 1] Japanese Patent Publication No. 2002-286716 [Patent Document 2] Japanese Patent Publication No. 2011-33544 [Patent Document 3] Japanese Patent Publication No. 2011-33546 [Patent Document 4] Japanese Patent Publication No. 2011-33547 [Patent Document 5] Japanese Patent Publication No. 2011-33548 [Overview of the project] [Problems that the invention aims to solve]

[0011] This invention provides a novel antigen for soy allergy. This invention also provides a diagnostic method and a diagnostic kit for soy allergy. Furthermore, this invention provides a pharmaceutical composition containing the antigen, and soy or soy products from which the antigen has been removed or reduced. This invention further provides a tester for determining the presence or absence of soy antigen in a subject. [Means for solving the problem]

[0012] To solve the above problems, the inventors diligently conducted research to identify the causative antigen of soy allergy. As a result, they succeeded in identifying a novel antigen to which IgE antibodies in the serum of patients with soy allergy specifically bind. Based on this finding, the present invention was completed.

[0013] In other words, in one embodiment, the present invention may be as follows.

[0014] [1] A diagnostic kit for soy allergy, containing at least one of the following proteins (1) to (8): (1)(1A) Bowman-Birk type proteinase inhibitor D-II or its variants, the following (1A-a)~ Any of the proteins (1A-e): (1A-a) In SEQ ID NO: 2, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; (1A-b) Amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 2 Contains protein; (1A-c) One or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 1 A protein containing an amino acid sequence encoded by a specified base sequence; (1A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 1 is classified A protein containing the amino acid sequence that is being targeted; or (1A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 1 and A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under liggent conditions; or (1B) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 17-20; (2)(2A) Ribulose bisphosphate carboxylase large chain (bisphosphate carboxylase large chain) or its variants, as described below (2A-a) ~(2A-e) proteins: (2A-a) In SEQ ID NO: 4, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; A protein comprising an amino acid sequence having 70% or more identity with the amino acid sequence represented by SEQ ID NO: 4 in (2A-b); ; a protein; (2A-c) A protein comprising an amino acid sequence encoded by a nucleotide sequence in which one or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO: 3; ; a protein; (2A-d) A protein comprising an amino acid sequence encoded by a nucleotide sequence having 70% or more identity with the nucleotide sequence represented by SEQ ID NO: 3; or ; a protein; (2A-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence represented by SEQ ID NO: 3; or ; a protein; (2B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 21 to 28; (3) (3A) Gamma-glutamyl hydrolase or a variant thereof, which is any of the following proteins (3A-a) to (3A-e): (3A-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 6; ; a protein; (3A-b) A protein comprising an amino acid sequence having 70% or more identity with the amino acid sequence represented by SEQ ID NO: 6; ; a protein; (3A-c) A protein comprising an amino acid sequence encoded by a nucleotide sequence in which one or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO: 5; ; a protein; (3A-d) A protein comprising an amino acid sequence encoded by a nucleotide sequence having 70% or more identity with the nucleotide sequence represented by SEQ ID NO: 5; or ; a protein; (3A-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence represented by SEQ ID NO: 5; or ; a protein; (3B) Proteins containing the amino acid sequence shown in SEQ ID NO: 29 or 30; (4)(4A) Guanine nucleotide-binding protein subunit beta-like protein or a variant thereof, which is any of the following proteins (4A-a) to (4A-e): (4A-a) In SEQ ID NO: 8, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; (4A-b) Amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 8 Contains protein; (4A-c) One or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 7 A protein containing an amino acid sequence encoded by a specified base sequence; (4A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 7 is classified as a coding scheme. A protein containing the amino acid sequence that is being targeted; or (4A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 7 and A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under liggent conditions; or (4B) Proteins containing the amino acid sequence shown in SEQ ID NO: 31 or 32; (5)(5A) Protein SLE3 or its variant, which is any of the following proteins (5A-a) to (5A-e): (5A-a) In SEQ ID NO: 10, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; (5A-b) Amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 10 Proteins containing; (5A-c) One or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 9 A protein containing an amino acid sequence encoded by a specified base sequence; (5A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 9 is classified as a coding scheme. A protein containing the amino acid sequence that is being targeted; or (5A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 9 and A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under liggent conditions; or (5B) Proteins containing the amino acid sequence shown in SEQ ID NO: 33 or 34; (6)(6A) Seed biotin-containing protein SBP65 or a variant thereof, which is any of the following proteins (6A-a) to (6A-e): (6A-a) In SEQ ID NO: 12, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; (6A-b) Amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 12 Proteins containing; (6A-c) One or more nucleotides are deleted, substituted, inserted, or attached in SEQ ID NO: 11 A protein containing an amino acid sequence encoded by the added base sequence; (6A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 11 A protein containing the amino acid sequence that is to be called; or (6A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 11 A protein containing an amino acid sequence encoded by nucleic acids that hybridizes under triggering conditions; or (6B) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 35-53; (7)(7A) Ferritin-2 or its variant, the following (7A- a) Any of the proteins from (7A-e): (7A-a) In SEQ ID NO: 14, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; (7A-b) Amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 14 Proteins containing; (7A-c) One or more nucleotides are deleted, substituted, inserted, or attached in SEQ ID NO: 13 A protein containing an amino acid sequence encoded by the added base sequence; (7A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 13 A protein containing the amino acid sequence that is to be called; or (7A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 13 A protein containing an amino acid sequence encoded by nucleic acids that hybridizes under triggering conditions; or (7B) Proteins containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 54-61. (8)(8A) Cell division cycle protein 48 homolog or a variant thereof, which is any of the following proteins (8A-a) to (8A-e): (8A-a) In SEQ ID NO: 16, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; (8A-b) Amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 16 Proteins containing; (8A-c) One or more nucleotides are deleted, substituted, inserted, or attached in SEQ ID NO: 15 A protein containing an amino acid sequence encoded by the added base sequence; (8A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 15 A protein containing the amino acid sequence that is to be called; or (8A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 15 A protein containing an amino acid sequence encoded by nucleic acids that hybridizes under triggering conditions; or (8B) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 62-68; The diagnostic kit comprising as an antigen.

[0015] [2] A diagnostic composition for soy allergy, comprising at least one of the proteins specified as (1) to (8) in [1] above as an antigen.

[0016] [3] A method for providing indicators for diagnosing a target soy allergy, comprising the following steps: (i) The sample obtained from the subject is brought into contact with the antigen, where the sample is a solution containing Ig antibodies; (ii) Detect the binding of IgE antibodies to the antigen in the sample obtained from the subject; (iii) If binding of the target IgE antibody to the antigen is detected, it provides an indicator that the subject has a soy allergy; The method comprising, wherein the antigen is at least one of the proteins identified as (1) to (8) in [1] above.

[0017] [4] A pharmaceutical composition comprising at least one of the proteins specified as (1) to (8) in [1] above.

[0018] [5] The pharmaceutical composition described in [4] above for the treatment of soy allergy.

[0019] [6] Soybeans or soybean products characterized in that an antigen is removed or reduced, wherein the antigen is at least one of the proteins specified as (1) to (8) in [1] above.

[0020] [7] A tester for determining the presence or absence of soybean antigen in a target object, characterized by containing an antibody that binds to at least one of the proteins identified as (1) to (8) in [1] above.

[0021] [8] An antigen derived from soybeans, which is at least one of the proteins specified as (1) to (8) in [1] above, and which causes an allergy to soybeans.

[0022] [9] A diagnostic kit for soy allergy, comprising at least one of the following proteins (1α) to (55α): (1α)(1αA1)endoplasmin homolog isoform X2 or a variant thereof, comprising any of the following proteins (1αA1-a) to (1αA1-e): (1αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 70; (1αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 70; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (1αA1-c)Sequence ID 69; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (1αA1-d)Sequence No. 69; or (1αA1-e) The amino acid encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 69. Proteins containing sequences; (1αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 71-87; (1αA2)endoplasmin homolog isoform X2 or its variant, which is any of the following proteins (1αA2-a) to (1αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (1αA2-a)Sequence No. 89; (1αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 89; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (1αA2-c)Sequence ID 88; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (1αA2-d)Sequence No. 88; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (1αA2-e)SEQ ID NO: 88; or (1αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 90-104; (2α)(2αA1)heat shock cognate protein 80 or its variants, the following One of the proteins (2αA1-a) to (2αA1-e): (2αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 106; (2αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 106; (2αA1-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 105; (2αA1-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in Sequence ID No. 105; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (2αA1-e)SEQ ID NO: 105; (2αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 107-120; (2αA2)heat shock protein 90-1 or its variant, wherein the following (2αA2- a) Any of the proteins from (2αA2-e): (2αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 122; (2αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 122; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (2αA2-c)Sequence ID 121; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (2αA2-d)Sequence No. 121; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (2αA2-e)Sequence ID 121; or (2αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 123-137; (2αA3)hypothetical protein GLYMA_02G302500 or its variant, which is any of the following proteins (2αA3-a) to (2αA3-e): (2αA3-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 139; (2αA3-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 139; (2αA3-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 138; (2αA3-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in Sequence ID No. 138; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (2αA3-e)Sequence ID 138; or (2αB3) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 140-153; (4α)(4αA)embryo-specific urease isoform X1 or its variant, the following One of the proteins (4αA-a) to (4αA-e): (4αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 155; (4αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 155; (4αA-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 154; (4αA-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in Sequence ID No. 154; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (4αA-e)SEQ ID NO: 154; or (4αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 156 and 157; (5α)(5αA)alpha-1,4 glucan phosphorylase L isozyme, chloroplastic / amyloplastic or its variants, which are any of the following proteins (5αA-a) to (5αA-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (5αA-a)Sequence ID 159; (5αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 159; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (5αA-c)Sequence ID 158; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (5αA-d)Sequence No. 158; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (5αA-e)SEQ ID NO: 158; or (5αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 160 and 161; (6α)(6αA1)aconitate hydratase 1 or its variant, wherein the following (6αA One of the proteins from 1-a) to (6αA1-e): (6αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 163; (6αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 163; (6αA1-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 162; (6αA1-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in Sequence ID No. 162; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (6αA1-e)SEQ ID NO: 162; (6αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 164-167; or (6αA2)aconitate hydratase 1 or its mutant, the following (6αA2-a) ~(6αA2-e) proteins: (6αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 169; (6αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 169; (6αA2-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 168; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (6αA2-d)Sequence No. 168; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (6αA2-e)Sequence ID 168; or (6αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 170-172; (7α)(7αA)methionine synthase or its mutant, wherein the following (7αA-a )~(7αA-e) proteins: (7αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 174; (7αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 174; (7αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 173; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (7αA-d)Sequence No. 173; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (7αA-e)SEQ ID NO: 173; or (7αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 175-187; (8α)(8αA)transketolase, chloroplastic, or a variant thereof, comprising any of the following proteins (8αA-a) to (8αA-e): (8αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 189; (8αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 189; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (8αA-c)Sequence ID 188; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (8αA-d)Sequence No. 188; or (8αA-e) Consists of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 188. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes with a nucleic acid under stringent conditions; or (8αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 190-199; (9α)(9αA)lysine-tRNA ligase, cytoplasmic-like isoform X2 or its variant The protein in question is one of the following (9αA-a) to (9αA-e): (9αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 201; (9αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 201; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (9αA-c)Sequence ID 200; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (9αA-d)Sequence No. 200; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (9αA-e)Sequence ID 200; or (9αB) A protein containing the amino acid sequence shown in Sequence ID No. 202; (10α)(10αA1)chaperonin CPN60-2, mitochondrial or its variant , one of the following proteins (10αA1-a) to (10αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (10αA1-a)Sequence No. 204; (10αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 204; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (10αA1-c)Sequence ID 203; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (10αA1-d)Sequence No. 203; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (10αA1-e)SEQ ID NO: 203; (10αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 205-218; (10αA2)chaperonin CPN60-2, mitochondrial-like isoform X1 or its variant The protein in question is one of the following (10αA2-a) to (10αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (10αA2-a)Sequence No. 220; (10αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 220; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (10αA2-c)SEQ ID NO: 219; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (10αA2-d)SEQ ID NO. 219; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (10αA2-e)SEQ ID NO: 219; or (10αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 221-231; (11α)(11αA)polyadenylate-binding protein 8 isoform X3 or its variant, which is any of the following proteins (11αA-a) to (11αA-e): (11αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 233; (11αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 233; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (11αA-c)Sequence ID 232; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (11αA-d)Sequence No. 232; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (11αA-e)SEQ ID NO: 232; or (11αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 234-237; (12α)(12αA)pyrophosphate-fructose 6-phosphate 1-phosphotransferase subunit alpha-like or a variant thereof, comprising any of the following proteins (12αA-a) to (12αA-e): (12αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 239; (12αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 239; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (12αA-c)Sequence ID 238; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (12αA-d)Sequence No. 238; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (12αA-e)SEQ ID NO: 238; or (12αB) A protein containing the amino acid sequence shown in Sequence ID No. 240; (13α)(13αA1)protein disulfide isomerase-like protein precursor or mutant thereof, which is any of the following proteins (13αA1-a) to (13αA1-e): (13αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 242; (13αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 242; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (13αA1-c)SEQ ID NO: 241; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (13αA1-d)Sequence No. 241; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (13αA1-e)SEQ ID NO: 241; (13αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 243-252; (13αA2)protein disulfide-isomerase or a variant thereof, wherein the following (13 One of the proteins αA2-a) to (13αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (13αA2-a)Sequence No. 254; (13αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 254; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (13αA2-c)SEQ ID NO: 253; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (13αA2-d)Sequence No. 253; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (13αA2-e)SEQ ID NO: 253; or (13αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 255-278; (14α)(14αA1)V-type proton ATPase subunit B 2 or its variant, which is any of the following proteins (14αA1-a) to (14αA1-e): (14αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 280; (14αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 280; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (14αA1-c)Sequence ID 279; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (14αA1-d)Sequence No. 279; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (14αA1-e)SEQ ID NO: 279; (14αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 281-284; (14αA2)V-type proton ATPase subunit B 2 or its variant, which is any of the following proteins (14αA2-a) to (14αA2-e): (14αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 286; (14αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 286; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (14αA2-c)SEQ ID NO: 285; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (14αA2-d)Sequence No. 285; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (14αA2-e)SEQ ID NO: 285; or (14αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 287-289; (15,16α)(15,16αA1)UTP-glucose-1-phosphate uridylyltransferase or a variant thereof, which is any of the following proteins (15,16αA1-a) to (15,16αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (15,16αA1-a)Sequence ID 291; (15,16αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 291; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (15,16αA1-c)Sequence ID 290; (15,16αA1-d) has a 70% or greater identity with the base sequence shown in SEQ ID NO. 290. A protein containing an amino acid sequence encoded by the base sequence; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (15,16αA1-e)SEQ ID NO: 290; (15,16αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 292-304; (15,16αA2)hypothetical protein GLYMA_02G241100 or its variant, which is any of the following proteins (15,16αA2-a) to (15,16αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (15,16αA2-a)Sequence No. 306; (15,16αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 306; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (15,16αA2-c)SEQ ID NO: 305; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (15,16αA2-d)Sequence No. 305; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (15,16αA2-e)SEQ ID NO: 305; or (15,16αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 307-318; (17α)(17αA1)guanosine nucleotide diphosphate dissociation inhibitor 2 or its variant, any of the following (17αA1-a) to (17αA1-e) Proteins: (17αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 320; (17αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 320; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (17αA1-c)Sequence ID 319; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (17αA1-d)Sequence No. 319; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (17αA1-e)SEQ ID NO: 319; (17αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 321-325; (17αA2)rab GDP dissociation inhibitor alpha-like or its variant, One of the following proteins (17αA2-a) to (17αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (17αA2-a)Sequence ID 327; (17αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 327; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (17αA2-c)Sequence ID 326; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (17αA2-d)Sequence No. 326; or (17αA2-e) The complementary nucleotide sequence to the nucleotide sequence shown in sequence number 326 A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes with a nucleic acid under stringent conditions; or (17αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 328-332; (18α)(18αA)hypothetical protein GLYMA_10G028300 or its variants, which are any of the following proteins (18αA-a) to (18αA-e): (18αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 334; (18αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 334; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (18αA-c)Sequence ID 333; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (18αA-d)Sequence No. 333; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (18αA-e)Sequence ID 333; or (18αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 335-339; (19α)(19αA)sucrose binding protein homolog S-64 or its variant, which is any of the following proteins (19αA-a) to (19αA-e): (19αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 341; (19αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 341; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (19αA-c)Sequence ID 340; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (19αA-d)SEQ ID NO: 340; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (19αA-e)SEQ ID NO: 340; or (19αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 342-346; (25α)(25αA1)succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial or its variant, of which (25αA1-a) to (25αA1-e) One of the proteins: Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (25αA1-a)Sequence ID 348; (25αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 348; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (25αA1-c)Sequence ID 347; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (25αA1-d)Sequence No. 347; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (25αA1-e)SEQ ID NO: 347; (25αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 349-356; (25αA2)succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial or This is a variant of the following protein (25αA2-a)~(25αA2-e): (25αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 358; (25αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 358; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (25αA2-c)Sequence ID 357; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (25αA2-d)Sequence No. 357; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (25αA2-e)SEQ ID NO: 357; or (25αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 359-365; (26α)(26αA1)phosphoglycerate kinase, cytosolic, or a variant thereof, comprising any of the following proteins (26αA1-a) to (26αA1-e): (26αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 367; (26αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 367; (26αA1-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 366; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (26αA1-d)Sequence No. 366; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (26αA1-e)SEQ ID NO: 366; (26αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 368-382; (26αA2)phosphoglycerate kinase, cytosolic, or a variant thereof, comprising any of the following proteins (26αA2-a) to (26αA2-e): (26αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 384; (26αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 384; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (26αA2-c)Sequence ID 383; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (26αA2-d)Sequence No. 383; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (26αA2-e)SEQ ID NO: 383; (26αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 385-392; (26αA3)phosphoglycerate kinase, cytosolic, or a variant thereof, comprising any of the following proteins (26αA3-a) to (26αA3-e): (26αA3-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 394; (26αA3-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 394; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (26αA3-c)SEQ ID NO: 393; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (26αA3-d)Sequence No. 393; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (26αA3-e)SEQ ID NO: 393; or (26αB3) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 395-409; (27α)(27αA1)alcohol dehydrogenase 1 or its mutants, the following ( One of the proteins 27αA1-a) to (27αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (27αA1-a)Sequence ID 411; (27αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 411; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (27αA1-c)SEQ ID NO: 410; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (27αA1-d)Sequence No. 410; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (27αA1-e)SEQ ID NO: 410; (27αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 412-416; (27αA2)alcohol dehydrogenase 1 or its mutant, wherein the following (27αA Proteins 2-a) to (27αA2-e): (27αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 418; (27αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 418; Proteins containing an amino acid sequence encoded by a nucleotide sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (27αA2-c)Sequence ID 417; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (27αA2-d)Sequence No. 417; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (27αA2-e)SEQ ID NO: 417; (27αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 419 and 420; (27αA3)alcohol dehydrogenase 1-like or its mutant, comprising any of the following proteins (27αA3-a) to (27αA3-e): (27αA3-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 422; (27αA3-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 422; Proteins containing an amino acid sequence encoded by a nucleotide sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (27αA3-c)SEQ ID NO: 421; (27αA3-d) A base that is 70% or more identical to the base sequence shown in Sequence ID No. 421 A protein containing an amino acid sequence encoded by the sequence; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (27αA3-e)SEQ ID NO: 421; or (27αB3) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 423-427; (28α)(28αA)35 kDa seed maturation protein isoform X1 or its mutants The protein present is one of the following (28αA-a) to (28αA-e): (28αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 429; (28αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 429; (28αA-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 428; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (28αA-d)Sequence No. 428; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (28αA-e)Sequence ID 428; or (28αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 430-433; (29-33α)(29-33αA1)glyceraldehyde-3-phosphate dehydrogenase isoform X1 or its variant, the following (29-33αA1-a)~(29-33αA 1-e) any of the proteins: (29-33αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 435; (29-33αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 435; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (29-33αA1-c)Sequence ID 434; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (29-33αA1-d)Sequence No. 434; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (29-33αA1-e)SEQ ID NO: 434; (29-33αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 436-439; (29-33αA2)glyceraldehyde-3-phosphate dehydrogenase, cytosolic or its A variant of the following protein (29-33αA2-a)~(29-33αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (29-33αA2-a)Sequence No. 441; (29-33αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 441; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (29-33αA2-c)Sequence ID 440; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (29-33αA2-d)Sequence No. 440; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (29-33αA2-e)SEQ ID NO: 440; (29-33αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 442-445; (29-33αA3)uncharacterized protein LOC100782924 or its variants, which are any of the following proteins (29-33αA3-a) to (29-33αA3-e): (29-33αA3-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 447; (29-33αA3-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 447; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (29-33αA3-c)SEQ ID NO: 446; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (29-33αA3-d)SEQ ID NO: 446; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (29-33αA3-e)SEQ ID NO: 446; or (29-33αB3) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 448-451; (34α)(34αA1)bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1-like or a variant thereof, comprising any of the following proteins (34αA1-a) to (34αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (34αA1-a)Sequence ID 453; (34αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 453; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (34αA1-c)Sequence ID 452; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (34αA1-d)Sequence No. 452; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (34αA1-e)SEQ ID NO: 452; (34αB1) A protein containing the amino acid sequence shown in SEQ ID NO: 454; (34αA2)uncharacterized protein LOC100803483 or its variant, which is any of the following proteins (34αA2-a) to (34αA2-e): (34αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 456; (34αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 456; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (34αA2-c)SEQ ID NO: 455; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (34αA2-d)Sequence No. 455; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (34αA2-e)SEQ ID NO: 455; or (34αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 457 and 458; (35α)(35αA1)elongation factor 1-alpha or its variants, the following One of the proteins (35αA1-a) to (35αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (35αA1-a)Sequence ID 460; (35αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 460; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (35αA1-c)Sequence ID 459; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (35αA1-d)Sequence No. 459; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (35αA1-e)SEQ ID NO: 459; (35αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 461-466; (35αA2)elongation factor 1-alpha or its variant, wherein the following (35α Proteins A2-a) to (35αA2-e): (35αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 468; (35αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 468; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (35αA2-c)Sequence ID 467; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (35αA2-d)Sequence No. 467; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (35αA2-e)SEQ ID NO: 467; (35αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 469-471; (35αA3)elongation factor-1A isoform X1 or its variant, the following (3 One of the proteins 5αA3-a) to (35αA3-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (35αA3-a)Sequence ID 473; (35αA3-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 473; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (35αA3-c)SEQ ID NO: 472; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (35αA3-d)Sequence No. 472; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (35αA3-e)SEQ ID NO: 472; or (35αB3) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 474-477; (36,37α)(36,37αA1) seed maturation protein PM34 or its variant, which is any of the following proteins (36,37αA1-a) to (36,37αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (36,37αA1-a)Sequence ID 479; (36,37αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 479; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (36,37αA1-c)Sequence ID 478; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (36,37αA1-d)Sequence No. 478; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (36,37αA1-e)SEQ ID NO: 478; (36,37αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 480-483; (36,37αA2)uncharacterized protein LOC100809384 or its variant, which is any of the following proteins (36,37αA2-a) to (36,37αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (36,37αA2-a)Sequence ID 485; (36,37αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 485; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (36,37αA2-c)Sequence ID 484; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (36,37αA2-d)Sequence No. 484; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (36,37αA2-e)SEQ ID NO: 484; (36,37αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 486 and 487; (36,37αA3)hypothetical protein GLYMA_10G155300 or its variant, which is any of the following proteins (36,37αA3-a) to (36,37αA3-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (36,37αA3-a)Sequence ID 489; (36,37αA3-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 489; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (36,37αA3-c)Sequence ID 488; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (36,37αA3-d)Sequence No. 488; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (36,37αA3-e)SEQ ID NO: 488; or (36,37αB3) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 490-493; (38α)(38αA)seed biotin-containing protein SBP65-like isoform X1 or its variant, which is any of the following proteins (38αA-a) to (38αA-e): (38αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 495; (38αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 495; (38αA-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 494; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (38αA-d)Sequence No. 494; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (38αA-e)SEQ ID NO: 494; or (38αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 496-513; (39α)(39αA)ATP synthase subunit O, mitochondrial-like, or a variant thereof, comprising any of the following proteins (39αA-a) to (39αA-e): (39αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 515; (39αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 515; (39αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 514; (39αA-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in Sequence ID No. 514; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (39αA-e)SEQ ID NO: 514; or (39αB) A protein containing the amino acid sequence shown in SEQ ID NO: 516; (40,56α)(40,56αA)P24 oleosin isoform A or its variant, One of the following proteins (40,56αA-a) to (40,56αA-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (40,56αA-a)Sequence ID 518; (40,56αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 518; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (40,56αA-c)SEQ ID NO: 517; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (40,56αA-d)Sequence No. 517; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (40,56αA-e)SEQ ID NO: 517; or (40,56αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 519 and 520; (41α)(41αA)uncharacterized protein At3g49720-like isoform X2 or its A mutant, which is one of the following proteins (41αA-a) to (41αA-e): (41αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 522; (41αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 522; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (41αA-c)Sequence ID 521; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (41αA-d)Sequence No. 521; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (41αA-e)SEQ ID NO: 521; or (41αB) A protein containing the amino acid sequence shown in Sequence ID No. 523; (42,43α)(42,43αA1)superoxide dismutase [Mn], mitochondrial or its mutant, comprising any of the following proteins (42,43αA1-a) to (42,43αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (42,43αA1-a)Sequence ID 525; (42,43αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 525; Proteins containing an amino acid sequence encoded by a nucleotide sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (42,43αA1-c)Sequence ID 524; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (42,43αA1-d)Sequence No. 524; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (42,43αA1-e)SEQ ID NO: 524; (42,43αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 526-531; (42,43αA2)hypothetical protein GLYMA_04G221300 or its variant, which is any of the following proteins (42,43αA2-a) to (42,43αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (42,43αA2-a)Sequence ID 533; (42,43αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 533; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (42,43αA2-c)Sequence ID 532; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (42,43αA2-d)Sequence No. 532; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (42,43αA2-e)SEQ ID NO: 532; or (42,43αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 534-539; (45α)(45αA1)uncharacterized protein LOC100499771 or its variant, which is any of the following proteins (45αA1-a) to (45αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (45αA1-a)Sequence ID 541; (45αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 541; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (45αA1-c)SEQ ID NO: 540; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (45αA1-d)SEQ ID NO: 540; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (45αA1-e)SEQ ID NO: 540; (45αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 542-545; (45αA2)hypothetical protein GLYMA_09G192800 or its variant, which is any of the following proteins (45αA2-a) to (45αA2-e): (45αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 547; (45αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 547; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (45αA2-c)SEQ ID NO: 546; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (45αA2-d)Sequence No. 546; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (45αA2-e)SEQ ID NO: 546; or (45αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 548-551; (46α)(46αA)seed maturation protein PM22 or its variants, which are any of the following proteins (46αA-a) to (46αA-e): (46αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 553; (46αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 553; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (46αA-c)Sequence ID 552; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (46αA-d)Sequence No. 552; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (46αA-e)SEQ ID NO: 552; or (46αB) A protein containing the amino acid sequence shown in SEQ ID NO: 554; (47α)(47αA1)eukaryotic translation initiation factor 5A-2 or its variant Isotopes that are any of the following proteins (47αA1-a) to (47αA1-e): (47αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 556; (47αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 556; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (47αA1-c)SEQ ID NO: 555; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (47αA1-d)Sequence No. 555; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (47αA1-e)SEQ ID NO: 555; (47αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 557 and 558; (47αA2)uncharacterized protein LOC100305510 or its variants, which are any of the following proteins (47αA2-a) to (47αA2-e): (47αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 560; (47αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 560; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (47αA2-c)SEQ ID NO: 559; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (47αA2-d)Sequence No. 559; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (47αA2-e)SEQ ID NO: 559; or (47αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 561-563; (48α)(48αA)uncharacterized protein LOC100306570 or its variant, which is any of the following proteins (48αA-a) to (48αA-e): (48αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 565; (48αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 565; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (48αA-c)Sequence ID 564; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (48αA-d)Sequence No. 564; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (48αA-e)SEQ ID NO: 564; or (48αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 566-570; (50α)(50αA)uncharacterized protein LOC100813859 or its variant, which is any of the following proteins (50αA-a) to (50αA-e): (50αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 572; (50αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 572; Proteins containing amino acid sequences encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (50αA-c)Sequence ID 571; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (50αA-d)Sequence No. 571; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (50αA-e)SEQ ID NO: 571; or (50αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 573-576; (51α)(51αA)14-3-3-like protein or its variant, wherein the following (51α Aa)~(51αA-e) proteins: (51αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 578; (51αA-b) The amino acid sequence shown in SEQ ID NO: 578 has more than 70% identity with the A Proteins containing amino acid sequences; (51αA-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 577; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (51αA-d)Sequence No. 577; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (51αA-e)Sequence ID 577; or (51αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 579-582; (52α)(52αA)probable fructokinase-4 or its mutant, the following (5 Proteins of any of the following types: 2αA-a) to (52αA-e): (52αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 584; (52αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 584; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (52αA-c)Sequence ID 583; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (52αA-d)Sequence No. 583; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (52αA-e)Sequence ID 583; or (52αB) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 585-588; (53α)(53αA1)triosephosphate isomerase isoform X1 or a variant thereof, which is any of the following proteins (53αA1-a) to (53αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (53αA1-a)Sequence ID 590; (53αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 590; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (53αA1-c)Sequence ID 589; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (53αA1-d)Sequence No. 589; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (53αA1-e)SEQ ID NO: 589; (53αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 591-597; (53αA2)triosephosphate isomerase, cytosolic, or a variant thereof, comprising any of the following proteins (53αA2-a) to (53αA2-e): (53αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 599; (53αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 599; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (53αA2-c)Sequence ID 598; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (53αA2-d)Sequence No. 598; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (53αA2-e)SEQ ID NO: 598; or (53αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 600-604; (54α)(54αA1)superoxide dismutase [Fe], chloroplastic isoform X1 or The mutant is one of the following proteins (54αA1-a) to (54αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (54αA1-a)Sequence ID 606; (54αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO. 606; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (53αA1-c)SEQ ID NO: 605; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (54αA1-d)SEQ ID NO. 605; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (54αA1-e)SEQ ID NO: 605; (54αB1) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 607-611; (54αA2)iron-superoxide dismutase or a mutant thereof, wherein the following (54αA Proteins 2-a) to (54αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (54αA2-a)Sequence ID 613; (54αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 613; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (54αA2-c)SEQ ID NO: 612; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (54αA2-d)Sequence No. 612; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (54αA2-e)SEQ ID NO: 612; or (54αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 614-616; or (55α)(55αA1) 51 kDa seed maturation protein precursor or its mutant, which is any of the following proteins (55αA1-a) to (55αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (55αA1-a)Sequence ID 618; (55αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 618; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (55αA1-c)SEQ ID NO: 617; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (55αA1-d)SEQ ID NO. 617; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (55αA1-e)SEQ ID NO: 617; (55αB1) A small number of amino acid sequences selected from the group consisting of sequence numbers 619-635 A protein that contains at least one protein; (55αA2)Lea protein precursor or mutant thereof, wherein the following (55αA2- a) Any of the proteins from (55αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (55αA2-a)Sequence ID 637; (55αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 637; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (55αA2-c)SEQ ID NO: 636; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (55αA2-d)SEQ ID NO. 636; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (55αA2-e)SEQ ID NO: 636; or (55αB2) A protein containing at least one amino acid sequence selected from the group consisting of sequence numbers 638-649; The diagnostic kit comprising as an antigen.

[0023]

[10] A diagnostic composition for soy allergy, comprising at least one of the proteins specified as (1α) to (55α) in [9] above as an antigen.

[0024]

[11] A method for providing indicators for diagnosing a target soy allergy, comprising the following steps: (i) The sample obtained from the subject is brought into contact with the antigen, where the sample is a solution containing Ig antibodies; (ii) Detect the binding of IgE antibodies to the antigen in the sample obtained from the subject; (iii) If binding of the target IgE antibody to the antigen is detected, it provides an indicator that the subject has a soy allergy; The method comprising, wherein the antigen is at least one of the proteins identified as (1α) to (55α) in [9] above.

[0025]

[12] A pharmaceutical composition comprising at least one of the proteins specified as (1α) to (55α) in [9] above.

[13] The pharmaceutical composition described in

[12] above for the treatment of soy allergy.

[0026]

[14] Soybeans or soybean products characterized in that an antigen is removed or reduced, wherein the antigen is at least one of the proteins identified as (1α) to (55α) in [9] above.

[0027]

[15] A tester for determining the presence or absence of soybean antigen in a target object, characterized by comprising an antibody that binds to at least one of the proteins identified as (1α) to (55α) in [9] above.

[0028]

[16] An antigen derived from soybeans, which is at least one of the proteins identified as (1α) to (55α) in [9] above, and which causes an allergy to soybeans. [Effects of the Invention]

[0029] The present invention can provide a novel antigen for soy allergies. Since a novel antigen (allergen component) that causes soy allergy has been identified, it is possible to provide a highly sensitive diagnostic method and diagnostic kit for soy allergy, a pharmaceutical composition containing the antigen, and soy or soy products from which the antigen has been removed or reduced. [Brief explanation of the drawing]

[0030] [Figure 1] Figure 1 is a photograph of a gel showing the electrophoresis pattern of proteins contained in soybeans, obtained by two-dimensional electrophoresis. The band on the left side of the photograph is the molecular weight marker band. [Figure 2] Figure 2 shows an immunoblot image of the two-dimensional electrophoresis pattern of proteins contained in soybeans, using serum from healthy subjects. [Figure 3A] Figure 3A is a photograph of an immunoblot using the serum of soybean allergy patient 1 against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The arrows indicate spots where the serum of this soybean allergy patient specifically reacted. [Figure 3B] Figure 3B is a photograph of an immunoblot using serum from soybean allergy patient 2 against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The arrows indicate spots where the serum of the soybean allergy patient specifically reacted. [Figure 3C] Figure 3C is a photograph of an immunoblot using the serum of soybean allergy patient 3 against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The arrows indicate spots where the serum of the soybean allergy patient specifically reacted. [Figure 3D]Figure 3D is a photograph of an immunoblot using serum from four patients with soy allergies, compared to a two-dimensional electrophoresis pattern of proteins contained in soybeans. The arrows indicate spots where the serum of these soy allergy patients specifically reacted. [Figure 4] Figure 4 shows an immunoblot image of the two-dimensional electrophoresis pattern of proteins contained in soybeans, using serum from healthy subjects. [Figure 5A] Figure 5A is a photograph of an immunoblot using the serum of soybean allergy patient 1α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots indicated by white lines or enclosed in squares are the spots where the serum of the soybean allergy patient specifically reacted. The numbers (n) indicate the number of each spot, corresponding to the spot designated as "nα" in this specification. [Figure 5B] Figure 5B is a photograph of an immunoblot using the serum of soybean allergy patient 2α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots indicated by white lines or enclosed in squares are the spots where the serum of the soybean allergy patient specifically reacted. The numbers (n) indicate the number of each spot, corresponding to the spot identified as "nα" in this specification. [Figure 5C] Figure 5C is a photograph of an immunoblot using the serum of soybean allergy patient 3α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. Spots indicated by white lines or enclosed in squares represent spots where the serum of the soybean allergy patient specifically reacted. The numbers (n) indicate the number of each spot, corresponding to the spot designated as "nα" in this specification. [Figure 5D] Figure 5D is a photograph of an immunoblot using the serum of soybean allergy patient 4α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots indicated by the white lines are those where the serum of the soybean allergy patient specifically reacted. The numbers (n) indicate the number of each spot, corresponding to the spot identified as "nα" in this specification. [Figure 5E]Figure 5E is a photograph of an immunoblot using the serum of soybean allergy patient 5α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots indicated by the white lines are those where the serum of the soybean allergy patient specifically reacted. The numbers (n) indicate the number of each spot, corresponding to the spot identified as "nα" in this specification. [Figure 5F] Figure 5F is a photograph of an immunoblot using the serum of soybean allergy patient 6α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. Spots indicated by white lines or enclosed in squares represent spots where the serum of the soybean allergy patient specifically reacted. The numbers (n) indicate the number of each spot, corresponding to the spot designated as "nα" in this specification. [Figure 5G] Figure 5G is a photograph of an immunoblot using the serum of soybean allergy patient 7α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots enclosed in squares indicate spots where the serum of the soybean allergy patient specifically reacted. The numbers (n) indicate the number of each spot, corresponding to the spot identified as "nα" in this specification. [Figure 5H] Figure 5H is a photograph of an immunoblot using the serum of soy allergy patient 8α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots indicated by white lines or enclosed in squares are the spots where the serum of the soy allergy patient specifically reacted. The numbers (n) indicate the number of each spot, corresponding to the spot identified as "nα" in this specification. [Figure 5I] Figure 5I is a photograph of an immunoblot using the serum of soybean allergy patient 9α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. Spots indicated by white lines or enclosed in squares represent spots where the serum of the soybean allergy patient specifically reacted. The number (n) indicates the number of each spot, corresponding to the spot identified as "nα" in this specification. [Modes for carrying out the invention]

[0031] The present invention will be described in detail below, but the present invention is not limited to these descriptions.

[0032] Unless otherwise defined herein, scientific and technical terms used in connection with the present invention shall have meanings that are generally understood by those skilled in the art.

[0033] In this specification, when an amino acid sequence is shown, it shall be represented by the single-letter abbreviation of the amino acid, which is well known to those skilled in the art, with the leftmost terminology being the amino terminology and the rightmost terminology being the carboxyl terminology.

[0034] In this specification, allergy refers to a condition in which a body exhibits an adverse hypersensitivity reaction when an antigen that has been sensitized to a particular antigen is reintroduced to that antigen. In many food allergies, antigen-specific IgE antibodies are produced in the blood and tissues. These IgE antibodies bind to mast cells or basophils. When an antigen specific to these IgE antibodies reintroduces into the body of an allergic patient, the antigen combines with the IgE antibodies bound to mast cells or basophils, and these IgE antibodies cross-link on the cell surface, producing the physiological effects of IgE antibody-antigen interaction. These physiological effects include the release of histamine, serotonin, heparin, eosinophil migration factors, or various leukotrienes. These released substances trigger allergic reactions caused by the combination of IgE antibodies and specific antigens. Allergic reactions to specific antigens manifest through the above pathway.

[0035] In this specification, allergy to soybeans refers to a condition in which an allergic reaction occurs in response to proteins or other substances contained in soybeans as antigens. Allergic reactions to soybeans can occur upon contact with or ingestion of antigens contained in soybeans. Generally, allergic reactions that occur upon ingestion of food are specifically called food allergies. Allergy to soybeans may also be a type of food allergy.

[0036] In this specification, an antigen is a substance that elicits an allergic reaction, and is also referred to as an allergen component. Antigens are preferably proteins.

[0037] In this specification, a protein is a molecule having a structure in which natural amino acids are linked by peptide bonds. The number of amino acids contained in a protein is not particularly limited, but a protein in which 2 to 50 amino acids are linked by peptide bonds is sometimes called a peptide. Furthermore, if optical isomers of an amino acid are possible, the L-form is indicated unless otherwise specified. The notation of amino acid sequences of proteins or peptides used in this specification is based on standard usage and notation commonly used in this industry, and is represented by a single letter of the amino acid, with the left direction being the amino-terminus direction and the right direction being the carboxy-terminus direction.

[0038] Identification of antigens Using the methods described above, we identified antigens that cause soy allergies in proteins contained in soybeans. Specifically, soybean proteins were subjected to two-dimensional electrophoresis under the following conditions.

[0039] For the first dimension of electrophoresis, isoelectric focusing was performed using an IPG gel Immobiline Drystrip (pH3-10NL) manufactured by GE Healthcare Biosciences Corporation (hereinafter abbreviated as GE). The gel length was within the range of 5-10 cm, the pH range of 3-10, and the pH gradient of the gel relative to the electrophoresis direction was such that, with the total length of the gel being 1, the gel length up to pH 5 was denoted as a, the gel length from pH 5 to 7 as b, and the gel length above pH 7 as c, where a was within the range of 0.15-0.3, b was within the range of 0.4-0.7, and c was within the range of 0.15-0.3. Specifically, isoelectric focusing was performed using IPG gel Immobiline Drystrip (pH3-10NL) manufactured by GE Healthcare Biosciences Corporation (hereinafter abbreviated as GE). The electrophoresis equipment used was IPGphor manufactured by GE. The electrophoresis equipment's current limit was set to 75 μA per gel, and the voltage program was as follows: (1) a constant voltage process was performed at 300 V for up to 750 Vhr (the current change during the 30 minutes of electrophoresis before the end of this process was 5 μA), (2) the voltage was gradually increased to 1000 V over 300 Vhr, (3) the voltage was further gradually increased to 5000 V over 4500 Vhr, and (4) the first dimension of isoelectric focusing was performed at a constant voltage of 5000 V until the total Vhr reached 12000.

[0040] For the second dimension of electrophoresis, a polyacrylamide gel was used, with the gel concentration at the proximal end of the electrophoresis direction set to 3-6%, and the gel concentration at the leading end of the electrophoresis direction set to be higher than that at the proximal end. Specifically, SDS-PAGE was performed using a NuPAGE 4-12% Bris-Tris Gels IPG well mini 1mm from Life Technologies. The electrophoresis equipment used was from Life Technologies. XCell SureLock Mini-Cell was used. 50 mM MOPS and 50 mM electrophoresis buffers were used. Tris base, 0.1% (w / v) SDS, and 1m MEDTA were used for electrophoresis at a constant voltage of 200V for approximately 45 minutes.

[0041] As a result, we revealed that in the gel obtained by performing two-dimensional electrophoresis on soy protein under the above conditions, the following spots specifically bind to IgE antibodies of patients with soy allergies. Spot 1: Molecular weight 1-30 kDa, pI 3.0-7.0 Spot 2: Molecular weight 30-75 kDa, pI 4.0-8.5 Spot 3: Molecular weight 20-50 kDa, pI 7.0-12.0 Spot 4: Molecular weight 20-50 kDa, pI 6.0-10.0 Spot 5: Molecular weight 2-30 kDa, pI 4.0-9.0 Spot 6: Molecular weight 50-100 kDa, pI 5.0-10.0 Spot 7: Molecular weight 5-37 kDa, pI 3.0-7.0 Spot 8: Molecular weight 75-200 kDa, pI 4.0-6.0 Spot 1α: Molecular weight 70-120 kDa, pI 2.5-6.5 Spot 2α: Molecular weight 50-120 kDa, pI 2.5-6.5 Spot 4α: Molecular weight 70-120 kDa, pI 3.5-7.5 Spot 5α: Molecular weight 70-170 kDa, pI 3.5-7.5 Spot 6α: Molecular weight 70-120 kDa, pI 3.5-7.5 Spot 7α: Molecular weight 50-120 kDa, pI 3.5-7.5 Spot 8α: Molecular weight 50-120 kDa, pI 3.5-8.5 Spot 9α: Molecular weight 50-120 kDa, pI 3.5-8.5 Spot 10α: Molecular weight 30-90 kDa, pI 3.5-7.5 Spot 11α: Molecular weight 30-90 kDa, pI 3.5-8.5 Spot 12α: Molecular weight 30-90 kDa, pI 3.5-8.5 Spot 13α: Molecular weight 30-90 kDa, pI 3.5-7.5 Spot 14α: Molecular weight 30-90 kDa, pI 2.5-6.5 Spot 15α, 16α: Molecular weight 20-90 kDa, pI 3.5-7.5 Spot 17α: Molecular weight 20-90 kDa, pI 3.5-7.5 Spot 18α: Molecular weight 30-90 kDa, pI 3.5-8.5 Spot 19α: Molecular weight 30-90 kDa, pI 3.5-8.5 Spot 25α: Molecular weight 20-90 kDa, pI 3.5-7.5 Spot 26α: Molecular weight 20-90 kDa, pI 3.5-7.5 Spot 27α: Molecular weight 20-90 kDa, pI 3.5-7.5 Spot 28α: Molecular weight 10-70 kDa, pI 3.5-7.5 Spots 29α, 30α, 31α, 32α, 33α: Molecular weight 10-70 kDa, pI 3.5-8.5 Spot 34α: Molecular weight 10-70 kDa, pI 5.5-10.5 Spot 35α: Molecular weight 20-90 kDa, pI 5.5-10.5 Spot 36α, 37α: Molecular weight 10-70 kDa, pI 3.5-8.5 Spot 38α: Molecular weight 70-170 kDa, pI 5.5-10.5 Spot 39α: Molecular weight 5-60 kDa, pI 5.5-10.5 Spot 40α, 56α: Molecular weight 5-60 kDa, pI 5.5-10.5 Spot 41α: Molecular weight 5-60 kDa, pI 5.5-10.5 Spot 42α, 43α: Molecular weight 5-60 kDa, pI 5.5-10.5 Spot 45α: Molecular weight 2-50 kDa, pI 3.5-7.5 Spot 46α: Molecular weight 2-50 kDa, pI 3.5-7.5 Spot 47α: Molecular weight 5-50 kDa, pI 3.5-7.5 Spot 48α: Molecular weight 2-50 kDa, pI 3.5-8.5 Spot 50α: Molecular weight 2-50 kDa, pI 2.5-6.5 Spot 51α: Molecular weight 5-60 kDa, pI 2.5-6.5 Spot 52α: Molecular weight 10-70 kDa, pI 3.5-7.5 Spot 53α: Molecular weight 5-60 kDa, pI 3.5-8.5 Spot 54α: Molecular weight 5-60 kDa, pI 3.5-8.5 Spot 55α: Molecular weight 30-90 kDa, pI 5.5-10.5 antigen (1) Spot 1 antigen Mass spectrometry analysis of spot 1 revealed the following amino acid sequence. DQSSSYDDDEYSK (Sequence ID 17) KSDQSSSYDDDEYSK (Sequence ID 18) SDQSSSYDDDEYSK (Sequence ID 19) SYDDDEYSK (Sequence ID 20) Furthermore, analysis of spot 1 by comparing the mass data obtained from the mass spectrometer with Uniprot protein data revealed that it was Bowman-Birk type proteinase inhibitor D-II (amino acid sequence: SEQ ID NO: 2, encoding nucleotide sequence: SEQ ID NO: 1).

[0042] Therefore, in this application, the antigens of spot 1 are (1A-a) to (1A-e) and (1B) is acceptable. (1A-a) In SEQ ID NO: 2, one or more amino acids are deleted, substituted, inserted, or added. A protein containing a specific amino acid sequence. (1A-b) The amino acid sequence is 70% or more identical to the sequence shown in SEQ ID NO: 2, preferably 75%. Proteins containing amino acid sequences of % or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. (1A-c) One or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 1 A protein containing an amino acid sequence encoded by a specified base sequence. (1A-d) The base sequence shown in Sequence ID No. 1 has a 70% or greater identity, preferably 75% or less. Proteins containing amino acid sequences encoded by the above, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, and 99% or more of the base sequence. (1A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 1 and A protein containing an amino acid sequence encoded by nucleic acids that hybridize under liggent conditions. (1B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 17-20, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. More preferably a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 17-19, preferably a protein comprising at least two or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs: 17-20 may have one or more amino acids deleted, substituted, inserted, or added.

[0043] The proteins listed above (1A-a) to (1A-e) and (1B) also include proteins in which the amino acid residues of the protein have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0044] The proteins (1A-a) to (1A-e) and (1B) described above may appear as spots in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 1 kDa to 30 kDa, preferably 2 kDa to 25 kDa, more preferably around 5 to 20 kDa, and an isoelectric point of 3.0 to 7.0, preferably 4.0 to 6.0.

[0045] Preferably, the antigen protein at spot 1 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 2 (Bowman-Birk type proteinase inhibitor D-II), or is the cause of allergy to soy.

[0046] (2) Spot 2 antigen Mass spectrometry analysis of spot 2 revealed the following amino acid sequence. ALRALRLEDLRIPTAYIK (Sequence ID 21) ALRLSGGDHVHAGTVVGK (Sequence ID 22) LEGEREITLGFVDLLRDDFVEK (Sequence ID 23) LRLEDLRIPTAYIK (SEQ ID NO: 24) LTYYTPDYETK (SEQ ID NO: 25) TDGLTSLDRYK (SEQ ID NO: 26) TFQGPPHGIQVERD (SEQ ID NO: 27) TFQGPPHGIQVERDK (SEQ ID NO: 28) In addition, for spot 2, when the mass data obtained from the mass spectrometer was compared and analyzed with the protein data of Uniprot, it was identified as Ribulose bisphosphate carboxylase large chain (amino acid sequence: SEQ ID NO: 4, nucleotide sequence encoding the same: SEQ ID NO: 3). Column: SEQ ID NO: 4, nucleotide sequence encoding the same: SEQ ID NO: 3).

[0047] Therefore, the antigen of spot 2 in the present application may be any one of the following (2A-a) to (2A-e) and (2B). (2A-a) A protein containing an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 4 A protein containing an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 4 (2A-b) A protein containing an amino acid sequence having 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, 99% or more identity with the amino acid sequence represented by SEQ ID NO: 4 A protein containing an amino acid sequence having 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, 99% or more identity with the amino acid sequence represented by SEQ ID NO: 4 (2A-c) A protein containing an amino acid sequence encoded by a nucleotide sequence in which one or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO: 3 A protein containing an amino acid sequence encoded by a nucleotide sequence in which one or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO: 3 (2A-d) A protein containing an amino acid sequence encoded by a nucleotide sequence having 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, 99% or more identity with the nucleotide sequence represented by SEQ ID NO: 3 A protein containing an amino acid sequence encoded by a nucleotide sequence having 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, 99% or more identity with the nucleotide sequence represented by SEQ ID NO: 3 (2A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence represented by SEQ ID NO: 3 and a protein encoded by the nucleic acid A protein containing an amino acid sequence encoded by nucleic acids that hybridize under liggent conditions. (2B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 21 to 28, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 21 to 28 may have one or more amino acids deleted, substituted, inserted, or added.

[0048] The proteins listed above (2A-a) to (2A-e) and (2B) also include proteins in which the amino acid residues of the protein have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0049] The proteins described in (2A-a) to (2A-e) and (2B) above may appear as spots in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 30 kDa to 75 kDa, preferably 37 kDa to 70 kDa, more preferably around 40 to 60 kDa, and an isoelectric point of 4.0 to 8.5, preferably 5.5 to 7.5.

[0050] Preferably, the antigen protein at spot 2 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 4 (Ribulose bisphosphate carboxylase large chain). It can cause or be a cause of allergy to soybeans.

[0051] (3) Spot 3 antigen Mass spectrometry analysis of spot 3 revealed the following amino acid sequence. APHTEDAIRVTQSTANFFISEARK (Sequence ID 29) FVESGGARVIPLIYNESPENLNK (Sequence ID 30) Furthermore, regarding spot 3, analysis of the mass data obtained from the mass spectrometer, compared with Uniprot protein data, identified it as gamma-glutamyl hydrolase (amino acid sequence: SEQ ID NO: 6, encoding nucleotide sequence: SEQ ID NO: 5).

[0052] Therefore, in this application, the antigen of spot 3 may be any of the following (3A-a) to (3A-e) and (3B). (3A-a) In SEQ ID NO: 6, one or more amino acids are deleted, substituted, inserted, or added. A protein containing a specific amino acid sequence. (3A-b) The amino acid sequence is 70% or more identical to the sequence shown in SEQ ID NO: 6, preferably 75%. Proteins containing amino acid sequences of % or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. (3A-c) One or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 5 A protein containing an amino acid sequence encoded by a specified base sequence, or a glycosylated protein thereof. (3A-d) The base sequence shown in Sequence ID No. 5 has a 70% or greater identity, preferably 75% or less. Proteins containing amino acid sequences encoded by the above, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, and 99% or more of the base sequence. (3A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 5 and A protein containing an amino acid sequence encoded by nucleic acids that hybridize under liggent conditions. (3B) A protein comprising the amino acid sequence shown in SEQ ID NO: 29 or 30, preferably a protein comprising the amino acid sequences shown in SEQ ID NO: 29 and 30. Herein, the amino acid sequence shown in SEQ ID NO: 29 and / or 30 may have one or more amino acids deleted, substituted, inserted or added.

[0053] The proteins of (3A-a) to (3A-e) and (3B) above also include proteins in which the amino acid residues of the protein are modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0054] The proteins of (3A-a) to (3A-e) and (3B) above may be proteins that appear as spots with a molecular weight of 20 kDa to 50 kDa, preferably a molecular weight of 25 kDa to 40 kDa, more preferably around a molecular weight of 25 to 37 kDa, and an isoelectric point of 7.0 to 12.0, preferably 8.0 to 10.0, on the gel when two-dimensional electrophoresis is performed under the conditions described in the item of "Identification of Antigen" above.

[0055] Preferably, the protein that is the antigen of spot 3 has the same activity as the protein (Gamma-glutamyl hydrolase) having the amino acid sequence of SEQ ID NO: 6, or causes allergy to soybeans.

[0056] (4) Spot 4 antigen As a result of performing sequence identification by mass spectrometry on spot 4, the following amino acid sequences were detected. IWDLESK (SEQ ID NO: 31) SIVEDLK (SEQ ID NO: 32) In addition, when the mass data obtained from the mass spectrometer for spot 4 was compared and analyzed with the protein data of Uniprot, it was identified as a guanine nucleotide-binding protein subunit beta-like protein (amino acid sequence: SEQ ID NO: 8, nucleotide sequence encoding it: SEQ ID NO: 7).

[0057] Therefore, the antigen of spot 4 in the present application may be any of the following (4A-a) to (4A-e) and (4B). (4A-a) One or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 8 A protein containing a specific amino acid sequence. (4A-b) The amino acid sequence is 70% or more identical to the sequence shown in SEQ ID NO: 8, preferably 75%. Proteins containing amino acid sequences of % or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. (4A-c) One or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 7 A protein containing an amino acid sequence encoded by a specified base sequence. (4A-d) The base sequence shown in Sequence ID No. 7 has a 70% or greater identity, preferably 75% or less. Proteins containing amino acid sequences encoded by the above, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, and 99% or more of the base sequence. (4A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 7 and A protein containing an amino acid sequence encoded by nucleic acids that hybridize under liggent conditions. (4B) A protein comprising the amino acid sequence shown in SEQ ID NO: 31 or 32, preferably a protein comprising the amino acid sequences shown in SEQ ID NO: 31 and 32. Herein, the amino acid sequence shown in SEQ ID NO: 31 and / or 32 may have one or more amino acids deleted, substituted, inserted or added.

[0058] The proteins listed above (4A-a) to (4A-e) and (4B) also include proteins in which the amino acid residues of the protein have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0059] The proteins described above (4A-a) to (4A-e) and (4B) may appear as spots in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 20 kDa to 50 kDa, preferably 25 kDa to 40 kDa, more preferably around 25 to 37 kDa, and an isoelectric point of 6.0 to 10.0, preferably 7.0 to 9.0.

[0060] Preferably, the antigen protein at spot 4 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 8 (Guanine nucleotide-binding protein subunit beta-like protein), or is the cause of allergy to soy.

[0061] (5) Spot 5 antigen Mass spectrometry analysis of spot 5 revealed the following amino acid sequence. QELDERARQGETVVPGGTGGK(Sequence ID 33) SGEERAQEEAIDIDESK (Sequence ID 34) Furthermore, regarding spot 5, analysis of the mass data obtained from the mass spectrometer, compared with Uniprot protein data, identified it as protein SLE3 (amino acid sequence: SEQ ID NO: 10, encoding nucleotide sequence: SEQ ID NO: 9).

[0062] Therefore, in this application, the antigen for spot 5 may be any of the following (5A-a) to (5A-e) and (5B). (5A-a) In SEQ ID NO: 10, one or more amino acids are deleted, substituted, inserted, or added. A protein containing a specific amino acid sequence. (5A-b) The amino acid sequence is 70% or more identical to the sequence shown in SEQ ID NO: 10, preferably 7 Proteins containing 5% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (5A-c) One or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 9 A protein containing an amino acid sequence encoded by a specified base sequence. (5A-d) The base sequence shown in Sequence ID No. 9 has a 70% or greater identity, preferably 75% or less. Proteins containing amino acid sequences encoded by the above, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, and 99% or more of the base sequence. (5A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 9 and A protein containing an amino acid sequence encoded by nucleic acids that hybridize under liggent conditions. (5B) A protein comprising the amino acid sequence shown in SEQ ID NO: 33 or 34, preferably a protein comprising the amino acid sequences shown in SEQ ID NO: 33 and 34. Herein, the amino acid sequence shown in SEQ ID NO: 33 and / or 34 may have one or more amino acids deleted, substituted, inserted or added.

[0063] The proteins listed above (5A-a) to (5A-e) and (5B) also include proteins whose amino acid residues have been modified by processes such as phosphorylation, glycosylation, aminoacylation, ring opening, and deamination.

[0064] The proteins (5A-a) to (5A-e) and (5B) described above may appear as spots in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 2 kDa to 30 kDa, preferably 3 kDa to 25 kDa, more preferably around 5 to 20 kDa, and an isoelectric point of 4.0 to 9.0, preferably 5.0 to 7.0.

[0065] Preferably, the antigen protein at spot 5 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 10 (Protein SLE3), or is the cause of allergy to soy.

[0066] (6) Spot 6 antigen Mass spectrometry analysis of spot 6 revealed the following amino acid sequence. AAAHVVEGAAGYAGHK (Sequence ID 35) AKDYTLQAAEK (Sequence ID 36) ARETHELGAHFESLADK (Sequence ID 37) ATAVGWAAAHFSAEK (Sequence ID 38) DTPQGSIEALQAGERVK(Sequence ID 39) DYTLQAAEK (Sequence ID 40) EEAQRELEAK (Sequence ID 41) EGTGSIVFTAIGETVSSAGEK(Sequence ID 42) ERGRESGGQVVAEK (Sequence ID 43) GLAASAGETAK (Sequence ID 44) GQQGYAVTK (Sequence ID 45) KPSQPQEAEERPSEGIGETVRQYAQK (Sequence ID 46) PAEIVQER (Sequence ID 47) PQGSIEALQAGERVK (Sequence ID 48) QAASETLNTTTQTAQEK (Sequence ID 49) SAGGTTASYVGEK (SEQ ID NO: 50) SLTSIGEK (SEQ ID NO: 51) TPQGSIEALQAGERVK(Sequence ID 52) VTDHAAANVVGNK (Sequence ID 53) Furthermore, regarding spot 6, analysis of the mass data obtained from the mass spectrometer, compared with Uniprot protein data, identified it as seed biotin-containing protein SBP65 (amino acid sequence: SEQ ID NO: 12, encoding nucleotide sequence: SEQ ID NO: 11).

[0067] Therefore, in this application, the antigen of spot 6 may be any of the following (6A-a) to (6A-e) and (6B). (6A-a) In SEQ ID NO: 12, one or more amino acids are deleted, substituted, inserted, or added. A protein containing a specific amino acid sequence. (6A-b) The amino acid sequence is 70% or more identical to the sequence shown in SEQ ID NO: 12, preferably 7 Proteins containing 5% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (6A-c) One or more nucleotides are deleted, substituted, inserted, or attached in SEQ ID NO: 11 A protein containing an amino acid sequence encoded by the added base sequence. (6A-d) The base sequence shown in SEQ ID NO: 11 has 70% or more identity, preferably 75%. Proteins containing amino acid sequences encoded by the above nucleotide sequences: 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, and 99% or more. (6A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 11 A protein containing an amino acid sequence encoded by nucleic acids that hybridizes under trigger conditions. (6B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 35-53, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, or all of the sequences of said amino acid sequence. More preferably a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 35-39, 41-44, 46, 48-50, 52, and 53, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs: 35-53 may have one or more amino acids deleted, substituted, inserted, or added.

[0068] The proteins listed above (6A-a) to (6A-e) and (6B) also include proteins in which the amino acid residues of the protein have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0069] The proteins described above (6A-a) to (6A-e) and (6B) may appear as spots in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 50 kDa to 100 kDa, preferably 55 kDa to 90 kDa, more preferably around 60 to 75 kDa, and an isoelectric point of 5.0 to 10.0, preferably 6.0 to 8.0.

[0070] Preferably, the antigen protein of spot 6 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 12 (Seed biotin-containing protein SBP65), or is the cause of allergy to soy.

[0071] (7) Spot 7 antigen Mass spectrometry analysis of spot 7 revealed the following amino acid sequence. AASNAPAPLAGVIFEPFQELKK (Sequence ID 54) ASNAPAPLAGVIFEPFQELKK (Sequence ID 55) FFKESSEEEREHAEQLIK (Sequence ID 56) GHGVWHFDQK (Sequence ID 57) IAEYVAQLRLVGK (Sequence ID 58) KIAEYVAQLRLVGK (Sequence ID 59) LLHDEDHV (Sequence ID 60) SNAPAPLAGVIFEPFQELKK (Sequence ID 61) Furthermore, regarding spot 7, analysis of the mass data obtained from the mass spectrometer by comparing it with Uniprot protein data revealed that ferritin-2 (amino acid) The sequence was identified as: Sequence ID 14, and the nucleotide sequence encoding it: Sequence ID 13.

[0072] Therefore, in this application, the antigen of spot 7 may be any of the following (7A-a) to (7A-e) and (7B). (7A-a) In SEQ ID NO: 14, one or more amino acids are deleted, substituted, inserted, or added. A protein containing a specific amino acid sequence. (7A-b) The amino acid sequence is 70% or more identical to the sequence shown in SEQ ID NO: 14, preferably 7 Proteins containing 5% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (7A-c) One or more nucleotides are deleted, substituted, inserted, or attached in SEQ ID NO: 13 A protein containing an amino acid sequence encoded by the added base sequence. (7A-d) The base sequence shown in SEQ ID NO: 13 has 70% or more identity, preferably 75%. Proteins containing amino acid sequences encoded by the above nucleotide sequences: 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, and 99% or more. (7A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 13 A protein containing an amino acid sequence encoded by nucleic acids that hybridizes under trigger conditions. (7B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 54 to 61, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, or all of the sequences of said amino acid sequence. More preferably a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 54 to 59 and 61, preferably a protein comprising at least 2, 3, 4, 5, 6, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 54 to 61 may have one or more amino acids deleted, substituted, inserted, or added.

[0073] The proteins listed above (7A-a) to (7A-e) and (7B) also include proteins in which the amino acid residues of the protein have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0074] The proteins described in (7A-a) to (7A-e) and (7B) above may appear as spots in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 5 kDa to 37 kDa, preferably 10 kDa to 30 kDa, more preferably around 15 to 25 kDa, and an isoelectric point of 3.0 to 7.0, preferably 4.0 to 6.0.

[0075] Preferably, the antigen protein of spot 7 has activity equivalent to that of the protein (Ferritin-2) having the amino acid sequence of SEQ ID NO: 14, or is the cause of allergy to soy.

[0076] (8) Spot 8 antigen Mass spectrometry analysis of spot 8 revealed the following amino acid sequence. AGESESNLRK (Sequence ID 62) GESEANVREIFDK (Sequence ID 63) GILLYGPPGSGK (Sequence ID 64) LAGESESNLRK (Sequence ID 65) LSDDVDLERIAK (Sequence No. 66) RELQETVQYPVEHPEK (Sequence ID 67) TVFIIGATNRPDIID(Sequence ID 68) Furthermore, analysis of spot 8 by comparing the mass data obtained from the mass spectrometer with Uniprot protein data revealed that it is a Cell division cycle protein 48 homolog (amino acid sequence: SEQ ID NO: 16, encoding nucleotide sequence: SEQ ID NO: 15).

[0077] Therefore, in this application, the antigen of spot 8 may be any of the following (8A-a) to (8A-e) and (8B). (8A-a) In SEQ ID NO: 16, one or more amino acids are deleted, substituted, inserted, or added. A protein containing a specific amino acid sequence. (8A-b) The amino acid sequence is 70% or more identical to the sequence shown in SEQ ID NO: 16, preferably 7 Proteins containing 5% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (8A-c) One or more nucleotides are deleted, substituted, inserted, or attached in SEQ ID NO: 15 A protein containing an amino acid sequence encoded by the added base sequence. (8A-d) The base sequence shown in SEQ ID NO: 15 has 70% or more identity, preferably 75%. Proteins containing amino acid sequences encoded by the above nucleotide sequences: 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, and 99% or more. (8A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 15 A protein containing an amino acid sequence encoded by nucleic acids that hybridizes under trigger conditions. (8B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 62 to 68, preferably a protein comprising at least 2, 3, 4, 5, 6, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 62 to 68 may have one or more amino acids deleted, substituted, inserted, or added.

[0078] The proteins listed above (8A-a) to (8A-e) and (8B) also include proteins in which the amino acid residues of the protein have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0079] The proteins (8A-a) to (8A-e) and (8B) described above may appear as spots in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 75 kDa to 200 kDa, preferably 100 kDa to 170 kDa, more preferably around 100 to 150 kDa, and an isoelectric point of 3.0 to 7.0, preferably 4.0 to 6.0.

[0080] Preferably, the antigen protein at spot 8 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 16 (Cell division cycle protein 48 homolog), or is the cause of allergy to soy.

[0081] (1α) Spot 1α antigen Mass spectrometry analysis of spot 1α revealed the following amino acid sequence. APQDLYESYYNANK (Sequence ID 71) AVLFVPPK (SEQ ID NO: 72) DFASRIYDSVK (Sequence ID 73) DIFLRELISNASDALDK (Sequence ID 74) DIFYITGTSK(SEQ ID NO: 75) DNVDDVK (Sequence ID 76) EDSKPETDAVNDDNDVK(Sequence ID 77) EEAGEYLEESK (Sequence ID 78) EQLENSPFLERLK (Sequence ID 79) EVLGEGDNTK (Sequence ID 80) EVTEEEYTK (Sequence ID 81) FYHSLAK (Sequence ID 82) IGAVPHGLSTDSDVVK (Sequence ID 83) LGIIEDATNRNRLAK (Sequence ID 84) NPEDEGVK (Sequence ID 85) SGTSAFVEK (Sequence ID 86) TSLDISPEATVEEEDDTEVEAESDAK (Sequence ID 87) APQDLYESYYNANK (Sequence ID 90) AVLFVPPK (SEQ ID NO: 91) DFASRIYDSVK (Sequence ID 92) DIFLRELISNASDALDK (Sequence ID 93) DIFYITGTSK(SEQ ID NO: 94) DNVDDVK (SEQ ID NO: 95) DVLGEGDNTK (Sequence ID 96) EEAGEYLQESK (Sequence ID 97) EQLENSPFLERLK (Sequence ID 98) EVTEEEYTK (Sequence ID 99) FYHSLAK (Sequence ID 100) IGAVPHGLSTDSDVVK (Sequence ID 101) LGIIEDATNRNRLAK (Sequence ID 102) NPEDEGVK (Sequence ID 103) SGTSAFVEK (Sequence ID 104) Furthermore, when the mass data obtained from the mass spectrometer for spot 1α was compared with the protein data from NCBI, endoplasmin homolog isoform X2 (amino acid sequence: SEQ ID NO: 70, encoding nucleotide sequence: SEQ ID NO: 69) was identified as a protein containing one of SEQ ID NOs: 71-87, and endoplasmin homolog isoform X2 (amino acid sequence: SEQ ID NO: 89, encoding nucleotide sequence: SEQ ID NO: 88) was identified as a protein containing one of SEQ ID NOs: 90-104.

[0082] Therefore, in this application, the antigen of spot 1α may be any protein selected from the group consisting of (1αA1-a) to (1αA1-e), (1αB1), (1αA2-a) to (1αA2-e), and (1αB2). (1αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 70. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (1αA1-b)Sequence ID 70. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (1αA1-c)Sequence ID 69. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (1αA1-d)Sequence ID 69, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (1αA1-e)Sequence Number 69. (1αB1) At least one amino acid sequence selected from the group consisting of sequence numbers 71-87 A protein containing, preferably a protein containing at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 71 to 87 may have one or more amino acids deleted, substituted, inserted, or added. (1αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 89. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (1αA2-b)Sequence ID 89. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (1αA2-c)Sequence ID 88. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (1αA2-d)Sequence ID 88, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (1αA2-e)Sequence Number 88. (1αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 90-104, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs: 90-104 may have one or more amino acids deleted, substituted, inserted, or added.

[0083] The proteins selected from the group consisting of (1αA1-a) to (1αA1-e), (1αB1), (1αA2-a) to (1αA2-e), and (1αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0084] Proteins selected from the group consisting of (1αA1-a) to (1αA1-e), (1αB1), (1αA2-a) to (1αA2-e), and (1αB2) may appear as spots on a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 70 to 120 kDa, preferably around 80 to 110 kDa, and an isoelectric point of 2.5 to 6.5, preferably 3.0 to 6.0.

[0085] Preferably, the antigen protein of spot 1α has activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 70 or 89 (endoplasmin homolog isoform X2), or is the cause of allergy to soy.

[0086] (2α) Spot 2α antigen Mass spectrometry analysis of spot 2α revealed the following amino acid sequence. ADLVNNLGTIARSGTK(Sequence ID 107) AVENSPFLEK (SEQ ID NO: 108) EDQLEYLEERRLK (Sequence ID 109) EEYAAFYK (Sequence ID 110) EGQNDIYYITGESK (Sequence ID 111) EIFLRELISNASDALDK (Sequence ID 112) FYEAFSK (Sequence ID 113) HFSVEGQLEFK (Sequence ID 114) IRFESLTDK (Sequence ID 115) LAELLRYHSTK (Sequence ID 116) LDESEDEK (Sequence ID 117) LGIHEDSQNK (Sequence ID 118) RAPFDLFDTRK (Sequence ID 119) VEDVDEDK (Sequence ID 120) ADLVNNLGTIARSGTK(Sequence ID 123) AVENSPFLEK (Sequence ID 124) EDQLEYLEERRLK (Sequence ID 125) EEYSAFYK (Sequence ID 126) EGQSDIYYITGESK (Sequence ID 127) EIFLRELISNASDALDK (Sequence ID 128) FYEAFSK (Sequence ID 129) HFSVEGQLEFK (Sequence ID 130) IAELLRYHSTK (Sequence ID 131) IRFESLTDK (Sequence ID 132) LDESEDEK (Sequence ID 133) LGIHEDSQNK (Sequence ID 134) RAPFDLFDTRK (Sequence ID 135) SGDELTSLK (Sequence ID 136) VEDVDEEK (Sequence ID 137) ADLVNNLGTIARSGTK(Sequence ID 140) AVENSPFLEK (SEQ ID NO: 141) EDQLEYLEERRLK (Sequence ID 142) EEYAAFYK (Sequence ID 143) EGQNDIYYITGESK (Sequence ID 144) EIFLRELISNASDALDK (SEQ ID NO: 145) FYEAFSK (Sequence ID 146) HFSVEGQLEFK (Sequence ID 147) IRFESLTDK (Sequence ID 148) LAELLRYHSTK (Sequence ID 149) LDESEDEK (Sequence ID 150) LGIHEDSQNK (Sequence ID 151) RAPFDLFDTK (Sequence ID 152) VEDVDEDK (Sequence ID 153) Furthermore, regarding spot 2α, analysis of the mass data obtained from the mass spectrometer, compared with NCBI protein data, revealed that it contains one of sequence numbers 107-120, specifically heat shock cognate protein 80 (amino acid sequence: sequence number 106, encoding nucleotide sequence: sequence number 105). Heat shock protein 90-1 (amino acid sequence: SEQ ID NO: 122, which encodes it) is a protein containing one of the sequence numbers 123-137. The protein GLYMA_02G302500 was identified as a protein containing the base sequence (Sequence ID: 121) or one of Sequence IDs 140-153.

[0087] Therefore, in this application, the antigen of spot 2α may be any protein selected from the group consisting of (2αA1-a)~(2αA1-e), (2αB1), (2αA2-a)~(2αA2-e), (2αB2), (2αA3-a)~(2αA3-e), and (2αB3). (2αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 106. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (2αA1-b)Sequence ID 106. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (2αA1-c)Sequence ID 105. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (2αA1-d)Sequence ID 105, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (2αA1-e)Sequence ID 105. (2αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 107-120, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs: 107-120 may have one or more amino acids deleted, substituted, inserted, or added. (2αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 122. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (2αA2-b)Sequence ID 122. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (2αA2-c)Sequence ID 121. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (2αA2-d)Sequence ID 121, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (2αA2-e)Sequence ID 121. (2αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 123-137, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs: 123-137 may have one or more amino acids deleted, substituted, inserted, or added. (2αA3-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 139. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (2αA3-b)Sequence ID 139. (2αA3-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 138. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (2αA3-d)Sequence ID 138, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (2αA3-e)Sequence ID 138. (2αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 140-153, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or all of the sequences of said amino acid sequence. Here In this case, the amino acid sequence shown in any of sequence numbers 140 to 153 may have one or more amino acids deleted, substituted, inserted, or added.

[0088] The proteins selected from the group consisting of (2αA1-a)~(2αA1-e), (2αB1), (2αA2-a)~(2αA2-e), (2αB2), (2αA3-a)~(2αA3-e), and (2αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0089] Proteins selected from the group consisting of (2αA1-a)~(2αA1-e), (2αB1), (2αA2-a)~(2αA2-e), (2αB2), (2αA3-a)~(2αA3-e), and (2αB3) may appear as spots on a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 50~120 kDa, preferably around 60~110 kDa, and an isoelectric point of 2.5~6.5, preferably 3.0~6.0.

[0090] Preferably, the antigen protein of spot 2α is a protein having the amino acid sequence of SEQ ID NO: 106 (heat shock cognate protein 80), or the amino acid sequence of SEQ ID NO: 122 Proteins with rows (heat shock protein 90-1) or amino acids of SEQ ID NO: 139 It has activity equivalent to that of the hypothetical protein GLYMA_02G302500, or it is a cause of allergy to soy.

[0091] (4α) Spot 4α antigen Mass spectrometry analysis of spot 4α revealed the following amino acid sequence. AGGALSIAFVSK (SEQ ID NO: 156) DGLIVSIGK (SEQ ID NO: 157) Furthermore, regarding spot 4α, analysis of the mass data obtained from the mass spectrometer, compared with NCBI protein data, revealed that it contains either sequence number 156 or 157, and is identified as embryo-specific urease isoform X1 (amino acid sequence: sequence number 155, the base sequence encoding it). (Sequence ID 154) was identified.

[0092] Therefore, in this application, the antigen of spot 4α may be any protein selected from the group consisting of (4αA-a) to (4αA-e) and (4αB). (4αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 155. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (4αA-b)Sequence ID 155. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (4αA-c)Sequence ID 154. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (4αA-d)Sequence ID 154, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (4αA-e)Sequence ID 154. (4αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 156 and 157, preferably a protein comprising both of said amino acid sequences. In this case, the amino acid sequence shown in either SEQ ID NO: 156 or 157 may have one or more amino acids deleted, substituted, inserted, or added.

[0093] The proteins selected from the group consisting of (4αA-a) to (4αA-e) and (4αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0094] The protein selected from the group consisting of (4αA-a) to (4αA-e) and (4αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 70 to 120 kDa, preferably around 80 to 110 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0.

[0095] Preferably, the antigen protein of spot 4α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 155 (embryo-specific urease isoform X1), and This can cause allergies to soybeans.

[0096] (5α) Spot 5α antigen Mass spectrometry analysis of spot 5α revealed the following amino acid sequence. AFATYVQAK (Sequence ID 160) QQEATTSLSSFTPDASSIASSIK (Sequence ID 161) Furthermore, when the mass data obtained from the mass spectrometer for spot 5α was compared with NCBI's protein data and analyzed, the protein alpha-1,4 glucan phosphorylase L isozyme, chloroplastic / amyloplastic (amino acid sequence: sequence number 159, encoding sequence: sequence number 158) was identified as containing either sequence number 160 or 161.

[0097] Therefore, in this application, the spot 5α antigen may be any protein selected from the group consisting of (5αA-a) to (5αA-e) and (5αB). (5αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 159. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (5αA-b)Sequence ID 159. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (5αA-c)Sequence ID 158. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (5αA-d)Sequence ID 158, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (5αA-e)Sequence ID 158. (5αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 160 and 161, preferably a protein comprising both of said amino acid sequences. Herein, the amino acid sequence represented by either SEQ ID NO: 160 or 161 may have one or more amino acids deleted, substituted, inserted, or added.

[0098] Select from the group consisting of (5αA-a) to (5αA-e) and (5αB) above. Proteins also include proteins in which the amino acid residues of the protein have been modified by processes such as phosphorylation, glycosylation, aminoacylation, ring opening, and deamination.

[0099] The protein selected from the group consisting of (5αA-a) to (5αA-e) and (5αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 70 to 170 kDa, preferably around 80 to 160 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0.

[0100] Preferably, the antigen protein of spot 5α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 159 (alpha-1,4 glucan phosphorylase L isozyme, chloroplastic / amyloplastic), or is the cause of allergy to soy.

[0101] (6α) Spot 6α antigen Mass spectrometry analysis of spot 6α revealed the following amino acid sequence. FLNGEVGPK (Sequence ID 164) LSVFDAAEK (Sequence ID 165) LSVFDVAEK (Sequence ID 166) TSLAPGSGVVTK (Sequence ID 167) FLNGEVGPK (SEQ ID NO: 170) LSVFDVAEK (Sequence ID 171) TSLAPGSGVVTK (Sequence ID 172) Furthermore, regarding spot 6α, analysis of the mass data obtained from the mass spectrometer, compared with NCBI protein data, revealed that it contains one of sequence numbers 164-167, specifically aconitate hydratase 1 (amino acid sequence: Aconitate hydratase 1 (amino acid sequence: SEQ ID NO: 169, encoding sequence: SEQ ID NO: 168) was identified as a protein containing either SEQ ID NO: 163 (the nucleotide sequence encoding it: SEQ ID NO: 162) or one of SEQ ID NOs: 170-172.

[0102] Therefore, in this application, the antigen of spot 6α may be any protein selected from the group consisting of (6αA1-a) to (6αA1-e), (6αB1), (6αA2-a) to (6αA2-e), and (6αB2). (6αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 163. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (6αA1-b)Sequence ID 163. (6αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 162. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (6αA1-d)Sequence ID 162, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (6αA1-e)Sequence ID 162. (6αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 164-167, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs: 164-167 is one in which one or more amino acids are deleted, substituted, inserted, or added. That's fine. (6αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 169. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (6αA2-b)Sequence ID 169. (6αA2-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 168. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (6αA2-d)Sequence ID 168, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (6αA2-e)Sequence ID 168. (6αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 170-172, preferably a protein comprising at least two or all of said amino acid sequences. Herein, the amino acid sequence represented by any of SEQ ID NOs: 170-172 may have one or more amino acids deleted, substituted, inserted, or added.

[0103] The proteins selected from the group consisting of (6αA1-a) to (6αA1-e), (6αB1), (6αA2-a) to (6αA2-e), and (6αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0104] Proteins selected from the group consisting of (6αA1-a) to (6αA1-e), (6αB1), (6αA2-a) to (6αA2-e), and (6αB2) may appear as spots on a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 70 to 120 kDa, preferably around 80 to 110 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0.

[0105] Preferably, the antigen protein of spot 6α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 163 or 169 (aconitate hydratase 1), and This can cause allergies to soybeans.

[0106] (7α) Spot 7α antigen Mass spectrometry analysis of spot 7α revealed the following amino acid sequence. AGITVIQIDEAALREGLPLRK (Sequence ID 175) ALGVDTVPVLVGPVTYLLLSKPAK (Sequence ID 176) DEAFFSGNAAALASRK (Sequence ID 177) DEVEDLEK (Sequence ID 178) ISEEEYVK (Sequence ID 179) IVEVNALAK (SEQ ID NO: 180) LIRNELAK (Sequence ID 181) LLSVFREGVK (Sequence ID 182) LVVSTSSSLLHTAVDLVNETK(Sequence ID 183) SFSLLSLLPK (Sequence ID 184) SSPRVTNEAVQK (Sequence ID 185) TLTSLNGVTAYGFDLVRGTNTLDLIK (Sequence ID 186) YGAGIGPGVYDIHSPRIPPTEEIADRINK(Sequence ID 187) Furthermore, regarding spot 7α, analysis of the mass data obtained from the mass spectrometer, compared with NCBI protein data, revealed that it contains one of sequence numbers 175-187, specifically methionine synthase (amino acid sequence: sequence number). 174, the base sequence encoding it (SEQ ID NO: 173), was identified.

[0107] Therefore, in this application, the antigen of spot 7α may be any protein selected from the group consisting of (7αA-a) to (7αA-e) and (7αB). (7αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 174. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (7αA-b)Sequence ID 174. (7αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 173. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (7αA-d)Sequence ID 173, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleic acid sequence complementary to the base sequence shown in (7αA-e)Sequence ID 173. (7αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 175-187, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs: 175-187 may have one or more amino acids deleted, substituted, inserted, or added.

[0108] The proteins selected from the group consisting of (7αA-a) to (7αA-e) and (7αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0109] The protein selected from the group consisting of (7αA-a) to (7αA-e) and (7αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 50 to 120 kDa, preferably around 60 to 110 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0.

[0110] Preferably, the antigen protein of spot 7α has activity equivalent to that of the protein (methionine synthase) having the amino acid sequence of SEQ ID NO: 174, or is effective against soybeans. It can cause allergies.

[0111] (8α) Spot 8α antigen Mass spectrometry analysis of spot 8α revealed the following amino acid sequence. AIGIDRFGASAPAGRIYK (Sequence ID 190) ALPTYTPESPADATRNLSQTNLNALAK (Sequence ID 191) ANSYSVHGSALGAK ​​(Sequence ID 192) ATADAALVEK (Sequence ID 193) DKPTLIK (Sequence ID 194) GGYTISDNSTGNKPDVILIGTGSELEIAAK (Sequence ID 195) LPQLPGTSIEGVEK (Sequence ID 196) NGNNGYDDIRAAIK (Sequence ID 197) SVNTIRFLAIDAVEK (Sequence ID 198) VTTTIGYGSPNK (Sequence ID 199) Furthermore, when the mass data obtained from the mass spectrometer for spot 8α was compared with NCBI's protein data and analyzed, transketolase, chloroplastic (amino acid sequence: sequence number 189, encoding nucleotide sequence: sequence number 188) was identified as a protein containing one of sequence numbers 190-199.

[0112] Therefore, in this application, the antigen of spot 8α may be any protein selected from the group consisting of (8αA-a) to (8αA-e) and (8αB). (8αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 189. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (8αA-b)Sequence ID 189. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (8αA-c)Sequence ID 188. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (8αA-d)Sequence ID 188, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (8αA-e)Sequence ID 188. (8αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 190-199, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs: 190-199 may have one or more amino acids deleted, substituted, inserted, or added.

[0113] The proteins selected from the group consisting of (8αA-a) to (8αA-e) and (8αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0114] The protein selected from the group consisting of (8αA-a) to (8αA-e) and (8αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 50 to 120 kDa, preferably around 60 to 110 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0.

[0115] Preferably, the antigen protein of spot 8α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 189 (transketolase, chloroplastic), or is the cause of allergy to soy.

[0116] (9α) Spot 9α antigen Mass spectrometry analysis of spot 9α revealed the following amino acid sequence. DLSSEEANQYLK (Sequence ID 202) Furthermore, regarding Spot 9α, the mass data obtained from the mass spectrometer was sent to NCBI's TAN Analysis by comparing with protein data revealed that the protein containing SEQ ID NO: 202 is lysine-tRNA ligase, cytoplasmic-like isoform X2 (amino acid sequence: SEQ ID NO: 201, which is coded The nucleotide sequence (SEQ ID NO: 200) was identified.

[0117] Therefore, in this application, the antigen of spot 9α may be any protein selected from the group consisting of (9αA-a) to (9αA-e) and (9αB). (9αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 201. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (9αA-b)Sequence ID 201. (9αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 200. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (9αA-d)Sequence ID 200, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (9αA-e)Sequence ID 200. (9αB) A protein containing the amino acid sequence shown in SEQ ID NO: 202. Herein, the amino acid sequence shown in SEQ ID NO: 202 may have one or more amino acids deleted, substituted, inserted, or added.

[0118] The proteins selected from the group consisting of (9αA-a) to (9αA-e) and (9αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0119] The protein selected from the group consisting of (9αA-a) to (9αA-e) and (9αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 50 to 120 kDa, preferably around 60 to 110 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0.

[0120] Preferably, the protein that is the antigen of spot 9α is equivalent in activity to the protein having the amino acid sequence of SEQ ID NO: 201 (lysine-tRNA ligase, cytoplasmic-like isoform X2). It can cause sexual activity or be a cause of allergy to soybeans.

[0121] (10α) Spot 10α antigen Mass spectrometry analysis of spot 10α revealed the following amino acid sequence. AAVEEGIVPGGGVALLYASSELDK (Sequence ID 205) DRVTDALNATK (Sequence ID 206) EGVITISDGK (Sequence ID 207) GRNVVIEQSFGAPK (Sequence ID 208) GVEELADAVK (Sequence ID 209) IGGASEAEVGEK (Sequence ID 210) IGVQIIQNALK (Sequence ID 211) ISSINAIVK (Sequence ID 212) LLEQNDPDLGYDAAK (Sequence ID 213) LQTANFDQK (Sequence ID 214) LSGGVAVLK (Sequence ID 215) NIGASLVK (Sequence ID 216) TGIIDPLK (Sequence ID 217) TPVHTIASNAGVEGAVVVGK (Sequence ID 218) AGIIDPLK (Sequence ID 221) DRVTDALNATK (Sequence ID 222) EGVITISDGK (Sequence ID 223) GRNVVIEQSFGAPK (Sequence ID 224) GVEELADAVK (Sequence ID 225) IGGASEAEVGEK (Sequence ID 226) IGVQIIQNALK (Sequence ID 227) ISSINAIVK (Sequence ID 228) LLEQENHDLGYDAAK (Sequence ID 229) LQTANFDQK (Sequence ID 230) LSGGVAVLK (Sequence ID 231) Furthermore, when the mass data obtained from the mass spectrometer for spot 10α was compared with the protein data from NCBI, it was found that the protein containing one of sequence numbers 205-218 was chaperonin CPN60-2, mitochondrial (amino acid sequence: sequence number 204, encoding nucleotide sequence: sequence number 203), and the protein containing one of sequence numbers 221-231 was chaperonin CPN60-2, Mitochondrial-like isoform X1 (chaperonin CPN60-2, mitochondrial-like isoform X1) (amino acid sequence: SEQ ID NO: 220, encoding nucleotide sequence: sequence) Number 219) was identified.

[0122] Therefore, in this application, the antigen of spot 10α may be any protein selected from the group consisting of (10αA1-a) to (10αA1-e), (10αB1), (10αA2-a) to (10αA2-e), and (10αB2). (10αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 204. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (10αA1-b)Sequence ID 204. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (10αA1-c)Sequence ID 203. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (10αA1-d)Sequence ID 203, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (10αA1-e)Sequence Number 203. (10αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 205 to 218, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 205 to 218 may have one or more amino acids deleted, substituted, inserted, or added. (10αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 220. (10αA2-b) The amino acid sequence shown in SEQ ID NO: 220 has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 9 Proteins containing 8% or more, or 99% or more, of the amino acid sequence. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (10αA2-c)Sequence ID 219. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (10αA2-d)Sequence ID 219, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (10αA2-e)SEQ ID NO: 219. (10αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 221-231, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 221-231 may have one or more amino acids deleted, substituted, inserted, or added.

[0123] The proteins selected from the group consisting of (10αA1-a) to (10αA1-e), (10αB1), (10αA2-a) to (10αA2-e), and (10αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0124] Proteins selected from the group consisting of (10αA1-a) to (10αA1-e), (10αB1), (10αA2-a) to (10αA2-e), and (10αB2) may appear as spots with a molecular weight of 30 to 90 kDa, preferably around 40 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0125] Preferably, the antigen protein of spot 10α has activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 204 (haperonin CPN60-2, mitochondrial) or a protein having the amino acid sequence of SEQ ID NO: 220 (chaperonin CPN60-2, mitochondrial-like isoform X1), or is the cause of allergy to soy.

[0126] (11α) Spot 11α antigen Mass spectrometry analysis of spot 11α revealed the following amino acid sequence. AIDVLNFTPLNGK(Sequence ID 234) FNNVFVK (Sequence ID 235) GFGFVNFANVDDAAK (Sequence ID 236) SGAANVFIK (Sequence ID 237) Furthermore, when the mass data obtained from the mass spectrometer for spot 11α was compared with NCBI's protein data and analyzed, polyadenylate-binding protein 8 isoform X3 (amino acid sequence: SEQ ID NO: 233, encoding nucleotide sequence: SEQ ID NO: 232) was identified as a protein containing one of SEQ ID NOs: 234-237.

[0127] Therefore, in this application, the antigen of spot 11α may be any protein selected from the group consisting of (11αA-a) to (11αA-e) and (11αB). (11αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 233. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (11αA-b)Sequence ID 233. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (11αA-c)Sequence ID 232. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (11αA-d)Sequence ID 232, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (11αA-e)Sequence Number 232. (11αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 234 to 237, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 234 to 237 may have one or more amino acids deleted, substituted, inserted, or added.

[0128] The proteins selected from the group consisting of (11αA-a) to (11αA-e) and (11αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0129] The protein selected from the group consisting of (11αA-a) to (11αA-e) and (11αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 30 to 90 kDa, preferably around 40 to 80 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0.

[0130] Preferably, the antigen protein of spot 11α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 233 (polyadenylate-binding protein 8 isoform X3), or is the cause of allergy to soy.

[0131] (12α) Spot 12α antigen Mass spectrometry analysis of spot 12α revealed the following amino acid sequence. VVGVPVTLNGDLK (Sequence ID 240) Furthermore, when the mass data obtained from the mass spectrometer for spot 12α was compared with the protein data from NCBI, it was found that the protein containing SEQ ID NO: 240 is pyrophosphate-fructose 6-phosphate 1-phosphotransferase subunit alpha-like (amino acid sequence: SEQ ID NO: 239, the base sequence encoding it: Column number 238) was identified.

[0132] Therefore, in this application, the antigen of spot 12α may be any protein selected from the group consisting of (12αA-a) to (12αA-e) and (12αB). (12αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 239. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (12αA-b)Sequence ID 239. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (12αA-c)Sequence ID 238. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (12αA-d)Sequence ID 238, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (12αA-e)Sequence No. 238. (12αB) A protein containing the amino acid sequence shown in SEQ ID NO: 240. Herein, the amino acid sequence shown in SEQ ID NO: 240 may have one or more amino acids deleted, substituted, inserted, or added.

[0133] The proteins selected from the group consisting of (12αA-a) to (12αA-e) and (12αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0134] The protein selected from the group consisting of (12αA-a) to (12αA-e) and (12αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 30 to 90 kDa, preferably around 40 to 80 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0.

[0135] Preferably, the antigen protein of spot 12α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 239 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha-like), or is the cause of allergy to soy. ru.

[0136] (13α) Spot 13α antigen Mass spectrometry analysis of spot 13α revealed the following amino acid sequence. AASILSSHDPPVVLAK (Sequence ID 243) DGNVAPFVK (Sequence ID 244) DLASQYDVRGYPTIK (Sequence ID 245) EDIIEFIEK (Sequence ID 246) EEQVPLIIIQHNDGK (Sequence ID 247) EFVLTLDHSNFHDTVSK (Sequence ID 248) FFKPNLEADHIPTWLK (Sequence No. 249) FFNSPNAK (SEQ ID NO: 250) FSGEEFDNFSALAEK (Sequence ID 251) FVEESSTPVVTVFNNDPSNHPFVAK (Sequence ID 252) AASILSSHDPPIVLAK (Sequence ID 255) DGHVAPFVK (Sequence ID 256) DLASQYDVK (Sequence ID 257) EDIIEFIEK (Sequence ID 258) EEQVPLIIIQHNDGK (Sequence ID 259) EFVLTLDHSNFHDTVSK (Sequence ID 260) FFKPNLEADHIPTWLK (Sequence ID 261) FFNSPNAK (Sequence ID 262) FSGEEFDNFSALAEK (Sequence ID 263) FVEESSTPVVTVFNNEPSNHPFVVK(Sequence ID 264) GFPTINILRNGGK (SEQ ID NO: 265) GPREADGIVDYLK(Sequence ID 266) LAPEYEK (Sequence ID 267) LDATANDIPSETFDVQGYPTVYFRSASGK (Sequence ID 268) LRSDYDFGHTLNAK (Sequence ID 269) LSQYDGGRTK (Sequence ID 270) QLAPILDEVAISYQNEADVVIAK (Sequence ID 271) QQGVSFLVGDVESSQGAFQYFGLK (Sequence ID 272) QSGPASTEIK (Sequence ID 273) SADEATAFIGENK (Sequence ID 274) SEPIPETNDEPVK (Sequence ID 275) VAIVGVFPK (Sequence ID 276) VVVGASLEDIVFK (Sequence ID 277) YREAAEQYK (Sequence ID 278) Furthermore, when the mass data obtained from the mass spectrometer for spot 13α was compared with NCBI's protein data and analyzed, it was found that the protein containing one of sequence numbers 243-252 was a protein disulfide isomerase-like protein precursor (amino acid sequence: sequence number 242, encoding nucleotide sequence: sequence number 241), and the protein containing one of sequence numbers 255-278 was a protein disulfide isomerase (amino acid sequence: sequence number 254, encoding nucleotide sequence: sequence number 254) 3) was identified.

[0137] Therefore, in this application, the antigen of spot 13α may be any protein selected from the group consisting of (13αA1-a) to (13αA1-e), (13αB1), (13αA2-a) to (13αA2-e), and (13αB2). (13αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 242. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (13αA1-b)Sequence ID 242. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (13αA1-c)Sequence ID 241. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (13αA1-d)Sequence ID 241, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (13αA1-e)Sequence ID 241. (13αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 243-252, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 243-252 may have one or more amino acids deleted, substituted, inserted, or added. (13αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 254. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (13αA2-b)Sequence ID 254. In (13αA2-c)Sequence ID 253, one or more nucleotides are deleted, substituted, or inserted. A protein containing an amino acid sequence encoded by an added or added base sequence. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (13αA2-d)Sequence ID 253, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (13αA2-e)SEQ ID NO: 253. (13αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 255 to 278, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 255 to 278 may have one or more amino acids deleted, substituted, inserted, or added.

[0138] The proteins selected from the group consisting of (13αA1-a) to (13αA1-e), (13αB1), (13αA2-a) to (13αA2-e), and (13αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0139] Proteins selected from the group consisting of (13αA1-a) to (13αA1-e), (13αB1), (13αA2-a) to (13αA2-e), and (13αB2) may appear as spots with a molecular weight of 30 to 90 kDa, preferably around 40 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0140] Preferably, the antigen protein of spot 13α is a protein having the amino acid sequence of SEQ ID NO: 242 (protein disulfide isomerase-like protein precursor) or a protein having the amino acid sequence of SEQ ID NO: 254 (protein disulfide-isomerase). It has equivalent activity, or it can cause allergies to soybeans.

[0141] (14α) Spot 14α antigen Mass spectrometry analysis of spot 14α revealed the following amino acid sequence. DHSDVSNQLYANYAIGK (Sequence ID 281) FQEIVNIRLGDGTTRRGQVLEVDGEK (Sequence ID 282) FTTVQFTGEVLK (Sequence ID 283) TLDQFYSRDASN (Sequence ID 284) FQEIVNIRLGDGTTRRGQVLEVDGEK (Sequence ID 287) FTTVQFTGEVLK (Sequence ID 288) TLDQFYSRDAGN (Sequence ID 289) Furthermore, when the mass data obtained from the mass spectrometer for spot 14α was compared with NCBI's protein data and analyzed, V-type proton ATPase subunit B2 (amino acid sequence: SEQ ID NO: 280, encoding nucleotide sequence: SEQ ID NO: 279) was identified as a protein containing one of SEQ ID NOs. 281-284, and V-type proton ATPase subunit B2 (amino acid sequence: SEQ ID NO: 286, encoding nucleotide sequence: SEQ ID NO: 285) was identified as a protein containing one of SEQ ID NOs. 287-289.

[0142] Therefore, in this application, the antigens of spot 14α are as follows: (14αA1-a)~(14 It may be any protein selected from the group consisting of αA1-e), (14αB1), (14αA2-a)~(14αA2-e), and (14αB2). (14αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 280. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (14αA1-b)Sequence ID 280. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (14αA1-c)Sequence ID 279. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (14αA1-d)Sequence ID 279, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (14αA1-e)SEQ ID NO: 279. (14αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 281 to 284, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 281 to 284 may have one or more amino acids deleted, substituted, inserted, or added. (14αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 286. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (14αA2-b)Sequence ID 286. A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (14αA2-c)Sequence ID 285. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (14αA2-d)Sequence ID 285, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (14αA2-e)SEQ ID NO: 285. (14αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 287 to 289, preferably a protein comprising at least two or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 287 to 289 may have one or more amino acids deleted, substituted, inserted, or added.

[0143] The proteins selected from the group consisting of (14αA1-a) to (14αA1-e), (14αB1), (14αA2-a) to (14αA2-e), and (14αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0144] Proteins selected from the group consisting of (14αA1-a) to (14αA1-e), (14αB1), (14αA2-a) to (14αA2-e), and (14αB2) may appear as spots with a molecular weight of 30 to 90 kDa, preferably around 40 to 80 kDa, and an isoelectric point of 2.5 to 6.5, preferably 3.0 to 6.0, on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0145] Preferably, the antigen protein of spot 14α has activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 280 or 286 (V-type proton ATPase subunit B 2), or is the cause of allergy to soy.

[0146] (15α, 16α) Spot 15α, 16α antigens Mass spectrometry analysis of spots 15α and 16α revealed the following amino acid sequences. AIGINVPRSRFLPVK (Sequence ID 292) ARENPENPSIELGPEFK (Sequence ID 293) ATSDLLLVQSDLYTLEDGFVIRNK(Sequence ID 294) EINGPEDL (Sequence ID 295) EYVFVANSDNLGAIVDLK (Sequence ID 296) ILNHLIQNK (Sequence ID 297) IQTPTDEVVVPYDTLAPTPEGSSEVK (Sequence ID 298) LDALLSQGK (Sequence ID 299) RLVEADALK (Sequence ID 300) SAVAGLNEISENEK (SEQ ID NO: 301) SIPSIVELDSLK (Sequence ID 302) VLQLETAAGAAIRFFDK (Sequence ID 303) VSNFLGRFK (Sequence ID 304) AIGINVPRSRFLPVK (Sequence ID 307) ATSDLLLVQSDLYTLEDGFVIRNK(Sequence ID 308) EYVFVANSDNLGAIVDLK (Sequence ID 309) ILNHLIQNK (Sequence ID 310) IQTPTDEVVVPYDTLAPTPDGSSDVK (Sequence ID 311) LDALLSQGK (Sequence ID 312) LEVPDGAVISDK (Sequence ID 313) RLVEADALK (Sequence ID 314) SAVAGLNEISESEK (SEQ ID NO: 315) SIPSIVELDSLK (Sequence ID 316) VLQLETAAGAAIRFFDK (Sequence ID 317) VSNFLGRFK (Sequence ID 318) Furthermore, when the mass data obtained from the mass spectrometer for spots 15α and 16α were compared with the protein data from NCBI, it was found that the protein containing one of sequence numbers 292-304 was UTP-glucose-1-phosphate uridylyltransferase (amino acid sequence: sequence number 2). 91, the base sequence encoding it: SEQ ID NO: 290), the hypothetical protein GLYMA_02G241100 (amino acid sequence: SEQ ID NO: 306, the base sequence encoding it) is a protein containing any of SEQ ID NOs: 307-318. Column: Sequence ID 305) was identified.

[0147] Therefore, in this application, the antigens of spots 15α and 16α may be any protein selected from the group consisting of (15,16αA1-a) to (15,16αA1-e), (15,16αB1), (15,16αA2-a) to (15,16αA2-e), and (15,16αB2). (15,16αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 291. The amino acid sequence shown in (15,16αA1-b)SEQ ID NO: 291 has a 70% or greater identity, preferably 75% or greater, 80% or greater, 85% or greater, 90% or greater, 95% or greater, or 97% or less identity. Proteins containing over 98% and over 99% of the amino acid sequence. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (15,16αA1-c)Sequence ID 290. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (15,16αA1-d)Sequence ID 290, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (15,16αA1-e)Sequence ID 290. (15,16αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 292-304, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 292-304 may have one or more amino acids deleted, substituted, inserted, or added. (15,16αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 306. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (15,16αA2-b)Sequence ID 306. (15.16αA2-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 305. (15,16αA2-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in SEQ ID NO: 305, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. (15,16αA2-e) A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 305. (15,16αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 307 to 318, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 307 to 318 may have one or more amino acids deleted, substituted, inserted, or added.

[0148] The proteins selected from the group consisting of (15,16αA1-a) to (15,16αA1-e), (15,16αB1), (15,16αA2-a) to (15,16αA2-e), and (15,16αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0149] Proteins selected from the group consisting of (15,16αA1-a) to (15,16αA1-e), (15,16αB1), (15,16αA2-a) to (15,16αA2-e), and (15,16αB2) may appear as spots with a molecular weight of 20 to 90 kDa, preferably around 30 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0150] Preferably, the antigen protein of spots 15α and 16α is a protein having the amino acid sequence of SEQ ID NO: 291 (UTP-glucose-1-phosphate uridylyltransferase). It has activity equivalent to that of the protein with the amino acid sequence of SEQ ID NO: 306 (hypothetical protein GLYMA_02G241100), or it is a cause of soy allergy.

[0151] (17α) Spot 17α antigen Mass spectrometry analysis of spot 17α revealed the following amino acid sequence. AVDGSFVFNK (Sequence ID 321) FFIYVQNYNESDPK (Sequence ID 322) VEFDEEGK (Sequence ID 323) VIDLSVDLSAASAAEE (Sequence ID 324) VVGVTSEGETAK (Sequence ID 325) AVDGSYVFNK (Sequence ID 328) FFIYVQNYNESDPK (Sequence ID 329) VEFDEEGK (Sequence ID 330) VIDLSVDLSAASAAEE (Sequence ID 331) VVGVTSEGETAK (Sequence ID 332) Furthermore, regarding spot 17α, analysis of the mass data obtained from the mass spectrometer, compared with NCBI protein data, revealed that it contains one of sequence numbers 321-325 and is identified as guanosine nucleotide diphosphate dissociation inhibitor 2 (amino acid sequence: Rab GDP dissociation inhibitor alpha-like (RAB GDP dissociation inhibitor alpha-like) is a protein containing one of the following: SEQ ID NO: 320 (the base sequence encoding it: SEQ ID NO: 319), SEQ ID NOs: 328-332 (amino acid sequence: sequence) Number 327, the base sequence encoding it (SEQ ID NO: 326), was identified.

[0152] Therefore, in this application, the antigen of spot 17α may be any protein selected from the group consisting of (17αA1-a) to (17αA1-e), (17αB1), (17αA2-a) to (17αA2-e), and (17αB2). (17αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 320. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (17αA1-b)Sequence ID 320. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (17αA1-c)Sequence ID 319. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (17αA1-d)Sequence ID 319, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (17αA1-e)Sequence ID 319. (17αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 321 to 325, preferably a protein comprising at least two, three, four, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 321 to 325 may have one or more amino acids deleted, substituted, inserted, or added. (17αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 327. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (17αA2-b)Sequence ID 327. (17αA2-c)Sequence ID 326 contains one or more nucleotides that have been deleted, substituted, or inserted. A protein containing an amino acid sequence encoded by an added or added base sequence. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (17αA2-d)Sequence ID 326, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (17αA2-e)Sequence ID 326. (17αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 328 to 332, preferably a protein comprising at least two, three, four, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 328 to 332 may have one or more amino acids deleted, substituted, inserted, or added.

[0153] The proteins selected from the group consisting of (17αA1-a) to (17αA1-e), (17αB1), (17αA2-a) to (17αA2-e), and (17αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0154] The protein selected from the group consisting of (17αA1-a) to (17αA1-e), (17αB1), (17αA2-a) to (17αA2-e), and (17αB2) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 20 to 90 kDa, preferably around 30 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0.

[0155] Preferably, the antigen protein of spot 17α is a protein having the amino acid sequence of SEQ ID NO: 320 (guanosine nucleotide diphosphate dissociation inhibitor 2 ) or a protein having the amino acid sequence of SEQ ID NO: 327 (rab GDP dissociation inhibitor alpha-like) has equivalent activity, or is the cause of allergy to soybeans. Yes.

[0156] (18α) Spot 18α antigen Mass spectrometry analysis of spot 18α revealed the following amino acid sequence. DFETRVETEGGRIRVLK (Sequence ID 335) DNIVSSLDNVAK (Sequence ID 336) GRAVLGLVSESETEK (Sequence ID 337) LFDQQNEGSIFAISREQVRALAPTK (Sequence ID 338) RPTISNGYGRLTEVGPDDDEK (Sequence ID 339) Furthermore, when the mass data obtained from the mass spectrometer for spot 18α was compared with NCBI's protein data and analyzed, it was determined that the protein containing one of sequence numbers 335-339 was hypothetical protein GLYMA_10G028300 (amino acid sequence: sequence number 334, encoding nucleotide sequence: sequence number 334). Number 333) was identified.

[0157] Therefore, in this application, the antigen of spot 18α may be any protein selected from the group consisting of (18αA-a) to (18αA-e) and (18αB). (18αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 334. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (18αA-b)Sequence ID 334. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (18αA-c)Sequence ID 333. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (18αA-d)Sequence ID 333, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (18αA-e)Sequence ID 333. (18αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 335 to 339, preferably a protein comprising at least two, three, four, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 335 to 339 may have one or more amino acids deleted, substituted, inserted, or added.

[0158] The proteins selected from the group consisting of (18αA-a) to (18αA-e) and (18αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0159] The protein selected from the group consisting of (18αA-a) to (18αA-e) and (18αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 30 to 90 kDa, preferably around 40 to 80 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0.

[0160] Preferably, the antigen protein of spot 18α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 334 (hypothetical protein GLYMA_10G028300), or is the cause of allergy to soy.

[0161] (19α) Spot 19α antigen Mass spectrometry analysis of spot 19α revealed the following amino acid sequence. AQFNIFSK (Sequence ID 342) DFSTRVETEGGSIRVLK (Sequence ID 343) DNIVSSLDNVAK (Sequence ID 344) GRAVLGLVRESETEK (Sequence ID 345) RPTFSNGYGRLTEVGPDDEK (Sequence ID 346) Furthermore, when the mass data obtained from the mass spectrometer for spot 19α was compared with NCBI's protein data and analyzed, sucrose binding protein homolog S-64 (amino acid sequence: SEQ ID NO: 341, encoding nucleotide sequence: SEQ ID NO: 340) was identified as a protein containing one of SEQ ID NOs: 342-346.

[0162] Therefore, in this application, the antigen of spot 19α may be any protein selected from the group consisting of (19αA-a) to (19αA-e) and (19αB). (19αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 341. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (19αA-b)Sequence ID 341. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (19αA-c)Sequence ID 340. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (19αA-d)Sequence ID 340, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (19αA-e)Sequence ID 340. (19αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 342 to 346, preferably a protein comprising at least two, three, four, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 342 to 346 may have one or more amino acids deleted, substituted, inserted, or added.

[0163] The proteins selected from the group consisting of (19αA-a) to (19αA-e) and (19αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0164] The protein selected from the group consisting of (19αA-a) to (19αA-e) and (19αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 30 to 90 kDa, preferably around 40 to 80 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0.

[0165] Preferably, the antigen protein of spot 19α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 341 (sucrose binding protein homolog S-64), or is the cause of allergy to soy.

[0166] (25α) Spot 25α antigen Mass spectrometry analysis of spot 25α revealed the following amino acid sequence. DLFPNENELVVK (Sequence ID 349) EIFSLRDTTQEDPREVTAAK (Sequence ID 350) LHGGTPANFLDVGGNASENQVVEAFK (Sequence ID 351) LNFDDNAAYRQK (SEQ ID NO: 352) SQILAGGRGLGTFK (Sequence ID 353) TDQVEDIAGK (Sequence ID 354) VPVVVRLEGTNVDQGK (Sequence ID 355) VVDGLALK (Sequence ID 356) DLFPNENELVVK (Sequence ID 359) EIFALRDTTQEDPREVTAAK (Sequence ID 360) LHGGTPANFLDVGGNASEGQVVEAFK (Sequence ID 361) LNFDDNAAYRQK (SEQ ID NO: 362) SQILAGGRGLGTFK (Sequence ID 363) TDQVEDIAGK (Sequence ID 364) VVDGLALK (Sequence ID 365) Furthermore, regarding spot 25α, the mass data obtained from the mass spectrometer was sent to NCBI. Analysis of the protein data revealed that the protein containing one of sequence numbers 349-356 is succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial (amino acid sequence: sequence number 348, encoding sequence: sequence number 347). Among the proteins containing one of sequence numbers 359-365, succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial (amino acid sequence: sequence number 358, encoding sequence: sequence number 357) was identified.

[0167] Therefore, in this application, the antigen of spot 25α may be any protein selected from the group consisting of (25αA1-a) to (25αA1-e), (25αB1), (25αA2-a) to (25αA2-e), and (25αB2). (25αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 348. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (25αA1-b)Sequence ID 348. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (25αA1-c)Sequence ID 347. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (25αA1-d)Sequence ID 347, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (25αA1-e)SEQ ID NO: 347. (25αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 349 to 356, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 349 to 356 may have one or more amino acids deleted, substituted, inserted, or added. (25αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 358. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (25αA2-b)Sequence ID 358. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (25αA2-c)Sequence ID 357. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (25αA2-d)Sequence ID 357, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (25αA2-e)SEQ ID NO: 357. (25αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 359 to 365, preferably a protein comprising at least 2, 3, 4, 5, 6, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 359 to 365 may have one or more amino acids deleted, substituted, inserted, or added.

[0168] The proteins selected from the group consisting of (25αA1-a)~(25αA1-e), (25αB1), (25αA2-a)~(25αA2-e), and (25αB2) are: This also includes proteins in which the amino acid residues of the protein have been modified by processes such as phosphorylation, glycosylation, aminoacylation, ring opening, and deamination.

[0169] Proteins selected from the group consisting of (25αA1-a) to (25αA1-e), (25αB1), (25αA2-a) to (25αA2-e), and (25αB2) may appear as spots with a molecular weight of 20 to 90 kDa, preferably around 30 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0170] Preferably, the antigen protein of spot 25α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 348 or 358 (succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial), or is the cause of allergy to soy. .

[0171] (26α) Spot 26α antigen Mass spectrometry analysis of spot 26α revealed the following amino acid sequence. AQGYSVGSSLVEEDK (Sequence ID 368) ELDYLVGAVSNPK (Sequence ID 369) FAADANSK (Sequence ID 370) FATGTEAIAK (Sequence ID 371) GVSLLLPTDVVIADK (Sequence ID 372) GVTTIIGGGDSVAAVEK (Sequence ID 373) IGVIESLLEK (Sequence ID 374) LASLADLYVNDAFGTAHRAHASTEGVAK (Sequence ID 375) LSLATTLLEK (Sequence ID 376) LVTQLPEGGVLLLENVRFYK (Sequence ID 377) NDPEFAK (Sequence ID 378) QLPGVLALDDA (Sequence ID 379) RPFAAIVGGSK (Sequence ID 380) VILSSHLGRPK (Sequence ID 381) YLTGYGAK (Sequence ID 382) ELDYLVGAVSNPK (Sequence ID 385) ELPGVLALDEAVPVAV (Sequence ID 386) FAVGTEAIAK (Sequence ID 387) GVSLLLPSDVVIADK (Sequence ID 388) GVTTIIGGGDSVAAVEK (Sequence ID 389) IGVIESLLEK (SEQ ID NO: 390) RPFAAIVGGSK (Sequence ID 391) VILSSHLGRPK (Sequence ID 392) AQGYSVGSSLVEEDK (Sequence ID 395) ELDYLVGAVSNPK (Sequence ID 396) FAADANSK (Sequence ID 397) FATGTEAIAK (Sequence ID 398) GVSLLLPTDVVIADK (Sequence ID 399) GVTTIIGGGDSVAAVEK (Sequence ID 400) IGVIESLLEK (SEQ ID NO: 401) LASLADLYVNDAFGTAHRAHASTEGVAK (Sequence ID 402) LSLATTLLEK (Sequence ID 403) LVAQLPEGGVLLLENVRFYK (Sequence ID 404) NDPEFAK (Sequence ID 405) QLPGVLALDDA (Sequence ID 406) RPFAAIVGGSK (Sequence ID 407) TFGEALDK (SEQ ID NO: 408) VILSSHLGRPK (Sequence ID 409) Furthermore, when the mass data obtained from the mass spectrometer for spot 26α was compared with NCBI's protein data and analyzed, the following proteins were identified: phosphoglycerate kinase, cytosolic (amino acid sequence: SEQ ID NO: 367, encoding nucleotide sequence: SEQ ID NO: 366) containing one of SEQ ID NOs: 385-392 (phosphoglycerate kinase, cytosolic (amino acid sequence: SEQ ID NO: 384, encoding nucleotide sequence: SEQ ID NO: 383)) and phosphoglycerate kinase, cytosolic (amino acid sequence: SEQ ID NO: 394, encoding nucleotide sequence: SEQ ID NO: 393) containing one of SEQ ID NOs: 395-409.

[0172] Therefore, in this application, the antigen of spot 26α may be any protein selected from the group consisting of (26αA1-a) to (26αA1-e), (26αB1), (26αA2-a) to (26αA2-e), (26αB2), (26αA3-a) to (26αA3-e), and (26αB3). (26αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 367. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (26αA1-b)Sequence ID 367. (26αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 366. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (26αA1-d)Sequence ID 366, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (26αA1-e)Sequence ID 366. (26αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 368 to 382, ​​preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 368 to 382 may have one or more amino acids deleted, substituted, inserted, or added. (26αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 384. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (26αA2-b)Sequence ID 384. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (26αA2-c)Sequence ID 383. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (26αA2-d)Sequence ID 383, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. (26αA2-e) Amino acids encoded by nucleic acids that hybridize under stringent conditions with nucleic acids consisting of a nucleic acid with a complementary base sequence to the base sequence shown in Sequence ID No. 383. A protein containing a sequence. (26αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 385 to 392, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 385 to 392 may have one or more amino acids deleted, substituted, inserted, or added. (26αA3-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 394. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (26αA3-b)Sequence ID 394. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (26αA3-c)Sequence ID 393. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (26αA3-d)Sequence ID 393, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (26αA3-e)Sequence ID 393. (26αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 395 to 409, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 395 to 409 may have one or more amino acids deleted, substituted, inserted, or added.

[0173] The proteins selected from the group consisting of (26αA1-a)~(26αA1-e), (26αB1), (26αA2-a)~(26αA2-e), (26αB2), (26αA3-a)~(26αA3-e), and (26αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0174] Proteins selected from the group consisting of (26αA1-a) to (26αA1-e), (26αB1), (26αA2-a) to (26αA2-e), (26αB2), (26αA3-a) to (26αA3-e), and (26αB3) may appear as spots with a molecular weight of 20 to 90 kDa, preferably around 30 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, on a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0175] Preferably, the antigen protein of spot 25α has activity equivalent to that of a protein (phosphoglycerate kinase, cytosolic) having the amino acid sequence of SEQ ID NO: 367, 384, or 394, or is the cause of allergy to soy.

[0176] (27α) Spot 27α antigen Mass spectrometry analysis of spot 27α revealed the following amino acid sequence. FGVNEFVNPK (Sequence ID 412) FITHTVPFSEINK (Sequence ID 413) GTFYGNYKPRTDLPSVVEK (Sequence ID 414) THPVNFLNERTLK (Sequence ID 415) VNPAAPLDK (Sequence ID 416) GTFYGHYRPRTDIPGVVEK (Sequence ID 419) INPNAPLDK (SEQ ID NO: 420) FGVNEFVNPK (Sequence ID 423) FITHTVPFSEINK (Sequence ID 424) GTFYGNYKPRTDLPSVVEK (Sequence ID 425) INPAAPLDK (Sequence ID 426) TAPINFLNERTLK (Sequence ID 427) Furthermore, regarding spot 27α, analysis of the mass data obtained from the mass spectrometer, compared with NCBI protein data, revealed that it contains one of sequence numbers 412-416, specifically alcohol dehydrogenase 1 (amino acid). Alcohol dehydrogenase 1 (amino acid sequence: SEQ ID NO: 418, encoding nucleotide sequence: SEQ ID NO: 417) was identified as a protein containing one of the sequences: SEQ ID NO: 411, encoding nucleotide sequence: SEQ ID NO: 410, SEQ ID NO: 419, or 420, and alcohol dehydrogenase 1-like (amino acid sequence: SEQ ID NO: 422, encoding nucleotide sequence: SEQ ID NO: 421) was identified as a protein containing one of the sequences: SEQ ID NO: 423 to 427.

[0177] Therefore, in this application, the antigen of spot 27α may be any protein selected from the group consisting of (27αA1-a)~(27αA1-e), (27αB1), (27αA2-a)~(27αA2-e), (27αB2), (27αA3-a)~(27αA3-e), and (27αB3). (27αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 411. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (27αA1-b)Sequence ID 411. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (27αA1-c)Sequence ID 410. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (27αA1-d)Sequence ID 410, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (27αA1-e)SEQ ID NO: 410. (27αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 412 to 416, preferably a protein comprising at least two, three, four, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 412 to 416 may have one or more amino acids deleted, substituted, inserted, or added. (27αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 418. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (27αA2-b)Sequence ID 418. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (27αA2-c)Sequence ID 417. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (27αA2-d)Sequence ID 417, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. (27αA2-e) Consists of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 417. A protein containing an amino acid sequence encoded by nucleic acids that hybridizes with nucleic acids under stringent conditions. (27αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 419 and 420, preferably a protein comprising both of said amino acid sequences. Herein, the amino acid sequence represented by either SEQ ID NOs. 419 or 420 may have one or more amino acids deleted, substituted, inserted, or added. (27αA3-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 422. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (27αA3-b)Sequence ID 422. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (27αA3-c)Sequence ID 421. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (27αA3-d)Sequence ID 421, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (27αA3-e)SEQ ID NO: 421. (27αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 423 to 427, preferably a protein comprising at least two, three, four, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 423 to 427 may have one or more amino acids deleted, substituted, inserted, or added.

[0178] The proteins selected from the group consisting of (27αA1-a)~(27αA1-e), (27αB1), (27αA2-a)~(27αA2-e), (27αB2), (27αA3-a)~(27αA3-e), and (27αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0179] Proteins selected from the group consisting of (27αA1-a) to (27αA1-e), (27αB1), (27αA2-a) to (27αA2-e), (27αB2), (27αA3-a) to (27αA3-e), and (27αB3) may appear as spots with a molecular weight of 20 to 90 kDa, preferably around 30 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0180] Preferably, the antigen protein of spot 27α is a protein having the amino acid sequence of SEQ ID NO: 411 or 418 (alcohol dehydrogenase 1) or SEQ ID NO: 42 It has activity equivalent to a protein with amino acid sequence 2 (alcohol dehydrogenase 1-like), or it is a cause of soy allergy.

[0181] (28α) Spot 28α antigen Mass spectrometry analysis of spot 28α revealed the following amino acid sequence. AGEYTDYAAQK (Sequence ID 430) DIGDINRTNERDEGYDLHDEANFK (Sequence ID 431) DLEDANRLRDNTFNTWK (Sequence ID 432) EAVVGKPHHEEVYAK (Sequence ID 433) Furthermore, regarding spot 28α, analysis of the mass data obtained from the mass spectrometer, compared with NCBI protein data, revealed that it contains one of sequence numbers 430-433 and is a 35 kDa seed mutation protein isoform X1 (amino acid sequence: sequence number 429). The nucleotide sequence that corresponds to this sequence (sequence number 428) was identified.

[0182] Therefore, in this application, the antigen of spot 28α may be any protein selected from the group consisting of (28αA-a) to (28αA-e) and (28αB). (28αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 429. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (28αA-b)Sequence ID 429. (28αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in sequence number 428. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (28αA-d)Sequence ID 428, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (28αA-e)Sequence ID 428. (28αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 430-433, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs: 430-433 may have one or more amino acids deleted, substituted, inserted, or added.

[0183] The proteins selected from the group consisting of (28αA-a) to (28αA-e) and (28αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0184] The protein selected from the group consisting of (28αA-a) to (28αA-e) and (28αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 10 to 70 kDa, preferably around 20 to 60 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0.

[0185] Preferably, the antigen protein of spot 28α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 422 (35 kDa seed maturation protein isoform X1). It can cause or lead to an allergy to soy.

[0186] (29α, 30α, 31α, 32α, 33α) Antigen spot 29α~33α Mass spectrometry analysis of spots 29α to 33α revealed the following amino acid sequences. EASYDEIK (Sequence ID 436) GILGYTEDDVVSTDFIGDSRSSIFDAK (Sequence ID 437) VIISAPSK (Sequence ID 438) VLPALNGK (Sequence ID 439) EASYDEIK (Sequence ID 442) GILGYTEDDVVSTDFVGDSRSSIFDAK (Sequence ID 443) VIISAPSK (Sequence ID 444) VLPALNGK (Sequence ID 445) AGISLNDNFVK (Sequence ID 448) GILGYTEDDVVSTDFVGDNRSSIFDAK (Sequence ID 449) VLPALNGK (Sequence ID 450) VVISAPSK (Sequence ID 451) Furthermore, when the mass data obtained from the mass spectrometer for spots 27α to 33α was compared with the protein data from NCBI, it was found that the protein containing one of sequence numbers 436 to 439 was glyceraldehyde-3-phosphate dehydrogenase isoform X1 (amino acid compound Glyceraldehyde-3-phosphate dehydrogenase, cytosolic protein containing any of the following: Sequence ID: 435, the base sequence encoding it: Sequence ID: 434), Sequence IDs 442-445. Uncharacterized protein LOC100782924 (amino acid sequence: SEQ ID NO: 447, encoding nucleotide sequence: SEQ ID NO: 446) was identified as a protein containing one of the following: ) (amino acid sequence: SEQ ID NO: 441, encoding nucleotide sequence: SEQ ID NO: 440), or SEQ ID NOs: 448-451.

[0187] Therefore, in this application, the antigens of spots 29α to 33α may be any protein selected from the group consisting of (29-33αA1-a) to (29-33αA1-e), (29-33αB1), (29-33αA2-a) to (29-33αA2-e), (29-33αB2), (29-33αA3-a) to (29-33αA3-e), and (29-33αB3). (29-33αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 435. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (29-33αA1-b)Sequence ID 435. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (29-33αA1-c)Sequence ID 434. (29-33αA1-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the nucleotide sequence shown in SEQ ID NO: 434. (29-33αA1-e) A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 434. (29-33αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 436-439, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 436-439 may have one or more amino acids deleted, substituted, inserted, or added. (29-33αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 441. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (29-33αA2-b)Sequence ID 441. (29-33αA2-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 440. (29-33αA2-d) The base sequence shown in sequence number 440 has a 70% or higher degree of identity, which is good. Proteins containing amino acid sequences encoded by nucleotide sequences of 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. Proteins containing amino acid sequences encoded by nucleic acids that hybridize under stringent conditions with nucleic acids consisting of nucleotide sequences complementary to the nucleotide sequence shown in (29-33αA2-e)SEQ ID NO: 440. (29-33αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 442 to 445, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 442 to 445 may have one or more amino acids deleted, substituted, inserted, or added. (29-33αA3-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 447. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (29-33αA3-b)Sequence ID 447. (29-33αA3-c) A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 446. (29-33αA3-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the nucleotide sequence shown in SEQ ID NO: 446. (29-33αA3-e) A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 446. (29-33αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 448-451, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 448-451 may have one or more amino acids deleted, substituted, inserted, or added.

[0188] The proteins selected from the group consisting of (29-33αA1-a)~(29-33αA1-e), (29-33αB1), (29-33αA2-a)~(29-33αA2-e), (29-33αB2), (29-33αA3-a)~(29-33αA3-e), and (29-33αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0189] Proteins selected from the group consisting of (29-33αA1-a)~(29-33αA1-e), (29-33αB1), (29-33αA2-a)~(29-33αA2-e), (29-33αB2), (29-33αA3-a)~(29-33αA3-e), and (29-33αB3) may appear as spots with a molecular weight of 10~70 kDa, preferably around 20~60 kDa, and an isoelectric point of 3.5~8.5, preferably 4.0~8.0, on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0190] Preferably, the antigen protein at spots 29α to 33α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 435 (glyceraldehyde-3-phosphate dehydrogenase isoform X1), the protein having the amino acid sequence of SEQ ID NO: 441 (glyceraldehyde-3-phosphate dehydrogenase, cytosolic), or the protein having the amino acid sequence of SEQ ID NO: 447 (uncharacterized protein LOC100782924), or is the cause of allergy to soy.

[0191] (34α) Spot 34α antigen Mass spectrometry analysis of spot 34α revealed the following amino acid sequence. LFLEEIARDIQK (Sequence ID 454) LFLEEIARDIQK (Sequence ID 457) TTFDAVIHFAGLK (SEQ ID NO: 458) Furthermore, when the mass data obtained from the mass spectrometer for spot 34α was compared with NCBI's protein data and analyzed, bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1-like (amino acid sequence: SEQ ID NO: 453, encoding nucleotide sequence: SEQ ID NO: 452) was identified as a protein containing SEQ ID NO: 454, and uncharacterized protein LOC100803483 (amino acid sequence: SEQ ID NO: 456, encoding nucleotide sequence: SEQ ID NO: 455) was identified as a protein containing either SEQ ID NO: 457 or 458.

[0192] Therefore, in this application, the antigen of spot 34α may be any protein selected from the group consisting of (34αA1-a) to (34αA1-e), (34αB1), (34αA2-a) to (34αA2-e), and (34αB2). (34αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 453. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (34αA1-b)Sequence ID 453. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (34αA1-c)Sequence ID 452. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (34αA1-d)Sequence ID 452, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (34αA1-e)SEQ ID NO: 452. (34αB1) A protein containing the amino acid sequence shown in SEQ ID NO: 454. Herein, the amino acid sequence shown in SEQ ID NO: 464 may have one or more amino acids deleted, substituted, inserted, or added. (34αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 456. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (34αA2-b)Sequence ID 456. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (34αA2-c)Sequence ID 455. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (34αA2-d)Sequence ID 455, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (34αA2-e)SEQ ID NO: 455. (34αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 457 and 458, preferably a protein comprising both of said amino acid sequences. In this context, the amino acid sequence shown in either SEQ ID NO: 457 or 458 may have one or more amino acids deleted, substituted, inserted, or added.

[0193] The proteins selected from the group consisting of (34αA1-a) to (34αA1-e), (34αB1), (34αA2-a) to (34αA2-e), and (34αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0194] Proteins selected from the group consisting of (34αA1-a) to (34αA1-e), (34αB1), (34αA2-a) to (34αA2-e), and (34αB2) may appear as spots with a molecular weight of 10 to 70 kDa, preferably around 20 to 60 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0, in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0195] Preferably, the antigen protein of spot 34α has activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 453 (bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1-like) or a protein having the amino acid sequence of SEQ ID NO: 456 (uncharacterized protein LOC100803483), or is the cause of allergy to soy.

[0196] (35α) Spot 35α antigen Mass spectrometry analysis of spot 35α revealed the following amino acid sequence. ARYDEIVK (Sequence ID 461) DGQTREHALLAFTLGVK (Sequence ID 462) EVSSYLK (Sequence ID 463) FSEILTK (Sequence ID 464) STTTGHLIYK (Sequence ID 465) THINIVVIGHVDSGK (Sequence ID 466) DGQTREHALLAFTLGVK (Sequence ID 469) EVSSYLK (Sequence ID 470) STTTGHLIYK (Sequence ID 471) ARYDEIVK (Sequence ID 474) DGQTREHALLSFTLGVK (Sequence ID 475) EVSSYLK (Sequence ID 476) STTTGHLIYK (Sequence ID 477) Furthermore, when the mass data obtained from the mass spectrometer for spot 35α was compared with the protein data from NCBI, it was found to be a protein containing one of sequence numbers 461-466, specifically elongation factor 1-alpha. ) (amino acid sequence: SEQ ID NO: 460, encoding nucleotide sequence: SEQ ID NO: 459), elongation factor 1-alpha (amino acid sequence: SEQ ID NO: 468, encoding nucleotide sequence: SEQ ID NO: 467) as a protein containing any of SEQ ID NOs: 469-471, elongation factor-1A isoform X1 (elongation factor-1A isoform X1) (amino acid sequence The sequence: SEQ ID NO: 473, the nucleotide sequence encoding it: SEQ ID NO: 472, was identified.

[0197] Therefore, in this application, the antigen for spot 35α is selected from the group consisting of (35αA1-a)~(35αA1-e), (35αB1), (35αA2-a)~(35αA2-e), (35αB2), (35αA3-a)~(35αA3-e), and (35αB3). It may be any of the selected proteins. (35αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 460. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (35αA1-b)Sequence ID 460. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (35αA1-c)Sequence ID 459. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (35αA1-d)Sequence ID 459, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (35αA1-e)Sequence ID 459. (35αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 461 to 466, preferably a protein comprising at least 2, 3, 4, 5, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 461 to 466 may have one or more amino acids deleted, substituted, inserted, or added. (35αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 468. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (35αA2-b)Sequence ID 468. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (35αA2-c)Sequence ID 467. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (35αA2-d)Sequence ID 467, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (35αA2-e)Sequence ID 467. (35αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 469 to 471, preferably a protein comprising at least two or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 469 to 471 may have one or more amino acids deleted, substituted, inserted, or added. (35αA3-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 473. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (35αA3-b)Sequence ID 473. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (35αA3-c)Sequence ID 472. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (35αA3-d)Sequence ID 472, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (35αA3-e)SEQ ID NO: 472. (35αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 474 to 477, preferably a protein comprising at least two, three, or all of said amino acid sequences. Herein, the amino acid sequence represented by any of SEQ ID NOs: 474 to 477 may have one or more amino acids deleted, substituted, inserted, or added.

[0198] The proteins selected from the group consisting of (35αA1-a)~(35αA1-e), (35αB1), (35αA2-a)~(35αA2-e), (35αB2), (35αA3-a)~(35αA3-e), and (35αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0199] Proteins selected from the group consisting of (35αA1-a) to (35αA1-e), (35αB1), (35αA2-a) to (35αA2-e), (35αB2), (35αA3-a) to (35αA3-e), and (35αB3) may appear as spots with a molecular weight of 20 to 90 kDa, preferably around 30 to 80 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0, on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0200] Preferably, the antigen protein of spot 35α is a protein having the amino acid sequence of SEQ ID NO: 460 or 468 (elongation factor 1-alpha) or SEQ ID NO: 4 A protein with a 73-amino acid sequence (elongation factor-1A isoform X1) equivalent to It is active or can cause allergies to soybeans.

[0201] (36α, 37α) Spot 36α, 37α antigens spot 36α、37α Mass spectrometry was used to identify the sequence, and the following amino acid sequence was detected. EGSSIINTTSVNAYK (Sequence ID 480) FPPQQQQTQPGK (Sequence ID 481) GAIVAYTRGLALQLVSK (Sequence ID 482) LLDYTSTK (Sequence ID 483) EGATVAFTYVK (Sequence ID 486) QVIDLVVK (Sequence ID 487) EGSSIINTTSVNAYK (Sequence ID 490) FPPQQQQTQPGK (Sequence ID 491) GAIVAYTRGLALQLVSK (Sequence ID 492) LLDYTSTK (Sequence ID 493) Furthermore, when the mass data obtained from the mass spectrometer for spots 36α and 37α were compared with NCBI's protein data and analyzed, the following proteins were identified: Seed maturation protein PM34 (amino acid sequence: SEQ ID NO: 479, encoding nucleotide sequence: SEQ ID NO: 478) as a protein containing one of SEQ ID NOs: 486 or 487 as an uncharacterized protein LOC100809384 (amino acid sequence: SEQ ID NO: 485, encoding nucleotide sequence: SEQ ID NO: 484) as a protein containing one of SEQ ID NOs: 449 to 493 as a hypocephalic protein GLYMA_10G155300 The hypothetical protein GLYMA_10G155300 (amino acid sequence: SEQ ID NO: 489, encoding sequence: SEQ ID NO: 488) was identified.

[0202] Therefore, in this application, the antigens of spots 36α and 37α are as follows: (36,37αA1-a) to (36,37αA1-e), (36,37αB1), and (36,37αA2-a). The protein may be any of the proteins selected from the group consisting of ~(36,37αA2-e), (36,37αB2), (36,37αA3-a)~(36,37αA3-e), and (36,37αB3). (36,37αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 479. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (36,37αA1-b)Sequence ID 479. A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (36,37αA1-c)Sequence ID 478. (36,37αA1-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the nucleotide sequence shown in SEQ ID NO: 478. (36,37αA1-e) A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 478. (36,37αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 480-483, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 480-483 may have one or more amino acids deleted, substituted, inserted, or added. (36,37αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 485. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (36,37αA2-b)Sequence ID 485. A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (36,37αA2-c)Sequence ID 484. (36,37αA2-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the nucleotide sequence shown in SEQ ID NO: 484. (36,37αA2-e) A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 484. (36,37αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 486 and 487, preferably a protein comprising both of said amino acid sequences. Herein, the amino acid sequence represented by either SEQ ID NOs. 486 or 487 may have one or more amino acids deleted, substituted, inserted, or added. (36,37αA3-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 489. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (36,37αA3-b)Sequence ID 489. A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (36,37αA3-c)Sequence ID 488. (36,37αA3-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the nucleotide sequence shown in SEQ ID NO: 488. (36,37αA3-e) A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 488. Proteins containing a amino acid sequence. (36,37αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 490-493, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 490-493 may have one or more amino acids deleted, substituted, inserted, or added.

[0203] The proteins selected from the group consisting of (36,37αA1-a)~(36,37αA1-e), (36,37αB1), (36,37αA2-a)~(36,37αA2-e), (36,37αB2), (36,37αA3-a)~(36,37αA3-e), and (36,37αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0204] Proteins selected from the group consisting of (36,37αA1-a)~(36,37αA1-e), (36,37αB1), (36,37αA2-a)~(36,37αA2-e), (36,37αB2), (36,37αA3-a)~(36,37αA3-e), and (36,37αB3) may appear as spots with a molecular weight of 10~70 kDa, preferably around 20~60 kDa, and an isoelectric point of 3.5~8.5, preferably 4.0~8.0, on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0205] Preferably, the antigen proteins of spots 36α and 37α have activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 479 (seed maturation protein PM34), a protein having the amino acid sequence of SEQ ID NO: 485 (uncharacterized protein LOC100809384), or a protein having the amino acid sequence of SEQ ID NO: 489 (hypothetical protein GLYMA_10G155300), or are the cause of allergy to soy.

[0206] (38α) Spot 38α antigen Mass spectrometry analysis of spot 38α revealed the following amino acid sequence. AAGSTTAQYVGEK (Sequence ID 496) ALATGWSAAHYSTEITVEGTK (Sequence ID 497) AQVAGSTTVQYAGEK (Sequence ID 498) DTIGRVDNITTPLAK (Sequence ID 499) DTIGSVDHITTPLAEK (Sequence ID 500) ERELASDRARSGNIVK (Sequence ID 501) ERYERANQATSEAAK (Sequence ID 502) GGGEEQGRRREEIGEENK (Sequence ID 503) GLAAAAAGENAK (Sequence ID 504) GRVVIESEK (Sequence ID 505) GVAEYAGQK (Sequence ID 506) LGSAAHTLVK (Sequence ID 507) QSSLEEIFK(Sequence ID 508) QSSLEEISK(Sequence ID 509) REQKPLGSIIGESFQGVQEK (Sequence ID 510) TGNETQDIGQK(Sequence ID 511) VIVAGEGEK (Sequence ID 512) VQQHQGEGGGGVLNAIGETIAEIAETTK(Sequence ID 513) Furthermore, regarding spot 38α, the mass data obtained from the mass spectrometer was sent to NCBI. Analysis by comparing with protein data revealed that seed biotin-containing protein SBP65-like isoform X1 (amino acid sequence: SEQ ID NO: 495, encoding nucleotide sequence: SEQ ID NO: 494) is a protein containing one of the sequence numbers 496-513.

[0207] Therefore, in this application, the antigen of spot 38α may be any protein selected from the group consisting of (38αA-a) to (38αA-e) and (38αB). (38αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 495. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (38αA-b)Sequence ID 495. (38αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 494. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (38αA-d)Sequence ID 494, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (38αA-e)Sequence ID 494. (38αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 496-513, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs: 496-513 may have one or more amino acids deleted, substituted, inserted, or added.

[0208] The proteins selected from the group consisting of (38αA-a) to (38αA-e) and (38αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0209] The protein selected from the group consisting of (38αA-a) to (38αA-e) and (38αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 70 to 170 kDa, preferably around 80 to 160 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0.

[0210] Preferably, the antigen protein of spot 38α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 495 (seed biotin-containing protein SBP65-like isoform X1), or is the cause of allergy to soy.

[0211] (39α) Spot 39α antigen Mass spectrometry analysis of spot 39α revealed the following amino acid sequence. ETLQEIIGSGAK (Sequence ID 516) Furthermore, regarding spot 39α, analysis of the mass data obtained from the mass spectrometer by comparing it with NCBI protein data revealed that the protein containing sequence number 516 is ATP synthase subunit O, mitochondrial-like (amino acid sequence: sequence number 515, the base sequence encoding it: sequence) Number 514) was identified.

[0212] Therefore, in this application, the antigen of spot 39α may be any protein selected from the group consisting of (39αA-a) to (39αA-e) and (39αB). (39αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 515. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (39αA-b)Sequence ID 515. (39αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 514. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (39αA-d)Sequence ID 514, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (39αA-e)Sequence ID 514. (39αB) A protein containing the amino acid sequence shown in SEQ ID NO: 516. Herein, the amino acid sequence shown in SEQ ID NO: 516 may have one or more amino acids deleted, substituted, inserted, or added.

[0213] The proteins selected from the group consisting of (39αA-a) to (39αA-e) and (39αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0214] The protein selected from the group consisting of (39αA-a) to (39αA-e) and (39αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 5 to 60 kDa, preferably around 10 to 50 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0.

[0215] Preferably, the antigen protein of spot 39α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 515 (ATP synthase subunit O, mitochondrial-like), or is the cause of allergy to soy.

[0216] (40α, 56α) Spot 40α, 56α antigens Mass spectrometry analysis of spots 40α and 56α revealed the following amino acid sequences. EVGQDIQSK (Sequence ID 519) RTTVTATTATA (Sequence ID 520) Furthermore, when the mass data obtained from the mass spectrometer for spots 40α and 56α were compared with NCBI protein data and analyzed, it was found that the protein containing either sequence number 519 or 520 was P24 oleosin isoform A. The amino acid sequence (sequence number 518, and the nucleotide sequence encoding it (sequence number 517)) was identified.

[0217] Therefore, in this application, the antigens of spots 40α and 56α may be any protein selected from the group consisting of (40,56αA-a) to (40,56αA-e) and (40,56αB). (40,56αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 518. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (40,56αA-b)Sequence ID 518. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (40,56αA-c)Sequence ID 517. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (40,56αA-d)Sequence ID 517, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (40,56αA-e)SEQ ID NO: 517. (40,56αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 519 and 520, preferably a protein comprising both of said amino acid sequences. Herein, the amino acid sequence represented by either SEQ ID NOs. 519 or 520 may have one or more amino acids deleted, substituted, inserted, or added.

[0218] The proteins selected from the group consisting of (40,56αA-a) to (40,56αA-e) and (40,56αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0219] Proteins selected from the group consisting of (40,56αA-a) to (40,56αA-e) and (40,56αB) above may appear as spots with a molecular weight of 5 to 60 kDa, preferably around 10 to 50 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0, in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0220] Preferably, the antigen proteins of spots 40α and 56α have activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 518 (P24 oleosin isoform A), or This can cause allergies to soybeans.

[0221] (41α) Spot 41α antigen Mass spectrometry analysis of spot 41α revealed the following amino acid sequence. NAYGGSMK (Sequence ID 523) Furthermore, when the mass data obtained from the mass spectrometer for spot 41α was compared with NCBI's protein data and analyzed, the protein containing sequence number 523, uncharacterized protein At3g49720-like isoform X2 (amino acid sequence: sequence number 522, nucleotide sequence encoding it: sequence number 521), was identified.

[0222] Therefore, in this application, the antigen of spot 41α may be any protein selected from the group consisting of (41αA-a) to (41αA-e) and (41αB). (41αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 522. (41αA-b) The amino acid sequence shown in SEQ ID NO: 522 has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, and 98% or more. A protein containing 99% or more of the amino acid sequence. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (41αA-c)Sequence ID 521. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (41αA-d)Sequence ID 521, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (41αA-e)Sequence ID 521. (41αB) A protein containing the amino acid sequence shown in Sequence ID No. 523. Herein, the amino acid sequence shown in Sequence ID No. 523 may have one or more amino acids deleted, substituted, inserted, or added.

[0223] The proteins selected from the group consisting of (41αA-a) to (41αA-e) and (41αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0224] The protein selected from the group consisting of (41αA-a) to (41αA-e) and (41αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 5 to 60 kDa, preferably around 10 to 50 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0.

[0225] Preferably, the antigen protein of spot 41α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 515 (ATP synthase subunit O, mitochondrial-like), or is the cause of allergy to soy.

[0226] (42α, 43α) Spot 42α, 43α antigens Mass spectrometry analysis of spots 42α and 43α revealed the following amino acid sequences. ALEQLQDAIAK (Sequence ID 526) HHQTYITNYNK (Sequence ID 527) NLAPVREGGGEPPK (Sequence ID 528) NVRPDYLK (Sequence ID 529) RLVVETTANQDPLVTK (Sequence ID 530) YASEVYEK (Sequence ID 531) ALEQLQDAVAK (SEQ ID NO: 534) HHQTYITNFNK (Sequence ID 535) NLAPVREGGGEPPK (Sequence ID 536) NVRPDYLK (Sequence ID 537) RLVVETTANQDPLVTK (Sequence ID 538) YASEVYEK (Sequence ID 539) Furthermore, when the mass data obtained from the mass spectrometer for spots 42α and 43α were compared with NCBI's protein data and analyzed, it was found that the protein containing one of sequence numbers 526-531 was superoxide dismutase [Mn], mitochondrial (amino acid sequence: sequence number 525, encoding nucleotide sequence: sequence number 524), and the protein containing one of sequence numbers 534-539 was hypothetical protein GLYMA_04G221300 (amino acid sequence: sequence number 533, encoding nucleotide sequence: sequence number 532) was identified.

[0227] Therefore, in this application, the antigens of spots 42α and 43α may be any protein selected from the group consisting of (42,43αA1-a) to (42,43αA1-e), (42,43αB1), (42,43αA2-a) to (42,43αA2-e), and (42,43αB2). (42,43αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 525. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (42,43αA1-b)Sequence ID 525. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (42,43αA1-c)Sequence ID 524. (42,43αA1-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the nucleotide sequence shown in SEQ ID NO: 524. (42,43αA1-e) A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 524. (42,43αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 526-531, preferably a protein comprising at least 2, 3, 4, 5, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 526-531 may have one or more amino acids deleted, substituted, inserted, or added. (42,43αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 533. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (42,43αA2-b)Sequence ID 533. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (42,43αA2-c)Sequence ID 532. (42,43αA2-d) A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the nucleotide sequence shown in SEQ ID NO: 532. (42,43αA2-e) A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 532. (42,43αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 534-539, preferably a protein comprising at least 2, 3, 4, 5, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 534-539 may have one or more amino acids deleted, substituted, inserted, or added.

[0228] The proteins selected from the group consisting of (42,43αA1-a)~(42,43αA1-e), (42,43αB1), (42,43αA2-a)~(42,43αA2-e), and (42,43αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0229] The above (42,43αA1-a)~(42,43αA1-e), (42,43αB1), (42,43αA2-a)~(42,43αA2-e), and (42,43αB2) The proteins selected from the above group may appear as spots in the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 5 to 60 kDa, preferably around 10 to 50 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0.

[0230] Preferably, the antigen proteins of spots 42α and 43α have activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 525 (superoxide dismutase [Mn], mitochondrial) or the protein having the amino acid sequence of SEQ ID NO: 533 (hypothetical protein GLYMA_04G221300), or are the cause of allergy to soy.

[0231] (45α) spot 45α antigen Mass spectrometry analysis of spot 45α revealed the following amino acid sequence. HVPGFIERAEELK (Sequence ID 542) RFALLVEDLK (Sequence ID 543) VANVESGGEFTISSAEEIIK (Sequence ID 544) YTNALGLELDLTDK (Sequence ID 545) HVPGFIERAEELK (SEQ ID NO: 548) RFALLVEDLK (Sequence ID 549) VANVESGGEFTISSAEEIIK (Sequence ID 550) YTNALGLELDLTDK (Sequence ID 551) Furthermore, when analyzing the mass data obtained from the mass spectrometer for Spot 45α by comparing it with NCBI's protein data, it was found that the protein containing one of sequence numbers 542-545 was uncharacterized protein LOC100499771 (amino acid sequence: sequence number 541, encoding nucleotide sequence: sequence number 540), and the protein containing one of sequence numbers 548-551 was hypothetical protein GLYMA_09G192800 (amino acid sequence: sequence number 540). The sequence (sequence number 547) and the nucleotide sequence encoding it (sequence number 546) were identified.

[0232] Therefore, in this application, the antigen of spot 45α may be any protein selected from the group consisting of (45αA1-a) to (45αA1-e), (45αB1), (45αA2-a) to (45αA2-e), and (45αB2). (45αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 541. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (45αA1-b)Sequence ID 541. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (45αA1-c)Sequence ID 540. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (45αA1-d)SEQ ID NO: 540, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (45αA1-e)SEQ ID NO: 540. (45αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 542 to 545, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 542 to 545 may have one or more amino acids deleted, substituted, inserted, or added. (45αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 547. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (45αA2-b)Sequence ID 547. A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (45αA2-c)Sequence ID 546. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (45αA2-d)Sequence ID 546, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (45αA2-e)SEQ ID NO: 546. (45αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 548 to 551, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 548 to 551 may have one or more amino acids deleted, substituted, inserted, or added.

[0233] The proteins selected from the group consisting of (45αA1-a) to (45αA1-e), (45αB1), (45αA2-a) to (45αA2-e), and (45αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0234] Proteins selected from the group consisting of (45αA1-a) to (45αA1-e), (45αB1), (45αA2-a) to (45αA2-e), and (45αB2) may appear as spots with a molecular weight of 2 to 50 kDa, preferably around 5 to 40 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, on a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0235] Preferably, the antigen protein of spot 45α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 541 (uncharacterized protein LOC100499771) or the protein having the amino acid sequence of SEQ ID NO: 547 (hypothetical protein GLYMA_09G192800), or is the cause of allergy to soy.

[0236] (46α) Spot 46α antigen Mass spectrometry analysis of spot 46α revealed the following amino acid sequence. DNVEYLAK (Sequence ID 554) Furthermore, when the mass data obtained from the mass spectrometer for spot 46α was compared with NCBI's protein data and analyzed, seed maturation protein PM22 (amino acid sequence: sequence number 553, encoding nucleotide sequence: sequence number 552) was identified as a protein containing sequence number 554.

[0237] Therefore, in this application, the antigen of spot 46α may be any protein selected from the group consisting of (46αA-a) to (46αA-e) and (46αB). (46αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 553. (46αA-b) The amino acid sequence shown in SEQ ID NO: 553 is more than 70% identical, which is preferable. This refers to proteins containing 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (46αA-c)Sequence ID 552. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (46αA-d)Sequence ID 552, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (46αA-e)Sequence ID 552. (46αB) A protein containing the amino acid sequence shown in SEQ ID NO: 554. Herein, the amino acid sequence shown in SEQ ID NO: 554 may have one or more amino acids deleted, substituted, inserted, or added.

[0238] The proteins selected from the group consisting of (46αA-a) to (46αA-e) and (46αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0239] The protein selected from the group consisting of (46αA-a) to (46αA-e) and (46αB) above may be a protein that appears as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 2 to 50 kDa, preferably around 5 to 40 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0.

[0240] Preferably, the antigen protein of spot 46α has activity equivalent to that of the protein having the amino acid sequence of sequence number 553 (seed maturation protein PM22), or is the cause of allergy to soy.

[0241] (47α) Spot 47α antigen Mass spectrometry analysis of spot 47α revealed the following amino acid sequence. LPTDESLLTQIK (Sequence ID 557) NGYIVIK (Sequence ID 558) NGYIVIK (Sequence ID 561) TYPQQAGTIRK (Sequence ID 562) VVEVSTSK(sequence number 563) Furthermore, regarding spot 47α, analysis of the mass data obtained from the mass spectrometer, compared with NCBI protein data, revealed that it contains either sequence number 557 or 558, and is identified as eukaryotic translation initiation factor 5A-2 (amino acid sequence: sequence number 557). 56. Uncharacterized protein LOC100305510 (amino acid sequence: SEQ ID NO: 560, encoding sequence: SEQ ID NO: 559) was identified as a protein containing one of the following: SEQ ID NOs: 561-563.

[0242] Therefore, in this application, the antigen of spot 47α may be any protein selected from the group consisting of (47αA1-a) to (47αA1-e), (47αB1), (47αA2-a) to (47αA2-e), and (47αB2). (47αA1-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 556. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown by (47αA1-b)Sequence ID 556. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (47αA1-c)Sequence ID 555. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (47αA1-d)SEQ ID NO: 555, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (47αA1-e)SEQ ID NO: 555. (47αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 557 and 558, preferably a protein comprising both of said amino acid sequences. Herein, the amino acid sequence represented by either SEQ ID NOs. 557 or 558 may have one or more amino acids deleted, substituted, inserted, or added. (47αA2-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 560. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (47αA2-b)Sequence ID 560. A protein containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (47αA2-c)Sequence ID 559. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (47αA2-d)Sequence ID 559, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (47αA2-e)SEQ ID NO: 559. (47αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 561 to 563, preferably a protein comprising at least two or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 561 to 563 may have one or more amino acids deleted, substituted, inserted, or added.

[0243] The proteins selected from the group consisting of (47αA1-a) to (47αA1-e), (47αB1), (47αA2-a) to (47αA2-e), and (47αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0244] Proteins selected from the group consisting of (47αA1-a) to (47αA1-e), (47αB1), (47αA2-a) to (47αA2-e), and (47αB2) may appear as spots with a molecular weight of 2 to 50 kDa, preferably around 5 to 40 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, on a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0245] Preferably, the antigen protein of spot 47α is a protein having the amino acid sequence of sequence number 556 (eukaryotic translation initiation factor 5A-2) or a compound It has activity equivalent to the protein with amino acid sequence number 560 (uncharacterized protein LOC100305510), or it is a cause of soy allergy.

[0246] (48α) Spot 48α antigen Mass spectrometry analysis of spot 48α revealed the following amino acid sequence. AEFPIFDK (sequence number 566) DARGNNVNLADYK (Sequence ID 567) DGNVVDRYAPTTSPLSIEK (Sequence ID 568) GGLFGDSIK (Sequence ID 569) VDVNGDNAAPLYK (SEQ ID NO: 570) Furthermore, when the mass data obtained from the mass spectrometer for spot 48α was compared with NCBI's protein data and analyzed, the uncharacterized protein LOC100306570 (amino acid sequence: SEQ ID NO: 565, encoding nucleotide sequence: SEQ ID NO: 564) was identified as a protein containing one of SEQ ID NOs: 566 to 570.

[0247] Therefore, in this application, the antigen of spot 48α may be any protein selected from the group consisting of (48αA-a) to (48αA-e) and (48αB). (48αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 565. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (48αA-b)Sequence ID 565. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (48αA-c)Sequence ID 564. A protein comprising an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (48αA-d)Sequence ID 564, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (48αA-e)Sequence ID 564. (48αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 566 to 570, preferably a protein comprising at least two, three, four, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 566 to 570 may have one or more amino acids deleted, substituted, inserted, or added.

[0248] The proteins selected from the group consisting of (48αA-a) to (48αA-e) and (48αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0249] The protein selected from the group consisting of (48αA-a) to (48αA-e) and (48αB) above may appear as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 2 to 50 kDa, preferably around 5 to 40 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0.

[0250] Preferably, the antigen protein of spot 48α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 565 (uncharacterized protein LOC100306570), or is the cause of allergy to soy.

[0251] (50α) Spot 50α antigen Mass spectrometry analysis of spot 50α revealed the following amino acid sequence. AADYLHNYQGDNTTAPSQPAESK(Sequence ID 573) LVAEAAQSALK (Sequence ID 574) QGIGQYVDK (Sequence ID 575) VADAAGDLLDAAGK (Sequence ID 576) Furthermore, when the mass data obtained from the mass spectrometer for spot 50α was compared with NCBI's protein data and analyzed, uncharacterized protein LOC100813859 (amino acid sequence: SEQ ID NO: 572, encoding nucleotide sequence: SEQ ID NO: 571) was identified as a protein containing one of SEQ ID NOs: 573 to 576.

[0252] Therefore, in this application, the antigen of spot 50α may be any protein selected from the group consisting of (50αA-a) to (50αA-e) and (50αB). (50αA-a) A protein containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in sequence number 572. A protein containing an amino acid sequence that is 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more identical to the amino acid sequence shown in (50αA-b)Sequence ID 572. A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (50αA-c)Sequence ID 571. A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (50αA-d)Sequence ID 571, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (50αA-e)Sequence ID 571. (50αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 573 to 576, preferably a protein comprising at least two, three, or all of the sequences of said amino acid sequence. Herein, the amino acid sequence represented by any of SEQ ID NOs. 573 to 576 may have one or more amino acids deleted, substituted, inserted, or added.

[0253] The proteins selected from the group consisting of (50αA-a) to (50αA-e) and (50αB) above also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring opening, deamination, etc.

[0254] The protein selected from the group consisting of (50αA-a) to (50αA-e) and (50αB) above may be a protein that appears as a spot on the gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above, with a molecular weight of 2 to 50 kDa, preferably around 5 to 40 kDa, and an isoelectric point of 2.5 to 6.5, preferably 3.0 to 6.0.

[0255] Preferably, the antigen protein of spot 50α has activity equivalent to that of the protein having the amino acid sequence of sequence number 572 (uncharacterized protein LOC100813859), or is the cause of allergy to soy.

[0256] (51α) Spot 51α antigen Mass spectrometry analysis of spot 51α revealed the following amino acid sequence. EAAESTLSAYK (Sequence ID 579) LLDSRLIPSASSGDSK (Sequence ID 580) QAFDEAIAELDTLGEESYK (Sequence ID 581) VSAAADNEELTVEERNLLSVAYK (Sequence ID 582) Furthermore, regarding spot 51α, analysis of the mass data obtained from the mass spectrometer, compared with NCBI protein data, revealed that it contains one of sequence numbers 579-582 and...

Claims

1. A diagnostic kit for soy allergy, containing at least one of the following proteins (1) to (8): (1) (1A) Bowman-Birk type proteinase inhibitor D-II or its variants, the following (1A-a) ~ Any of the proteins (1A-e): (1A-a) In SEQ ID NO: 2, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; (1A-b) Amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 2 Proteins containing; (1A-c) One or more nucleotides are deleted, substituted, inserted, or attached in SEQ ID NO: 1 A protein containing an amino acid sequence encoded by the added base sequence; (1A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 1 A protein containing the amino acid sequence that is to be used; or (1A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 1 and A protein comprising an amino acid sequence encoded by nucleic acids that hybridize under triggering conditions; or (1B) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 17-20; (2) (2A) Ribulose bisphosphate carboxylase large chain A bisphosphate carboxylase (large chain) or a variant thereof, which is any of the following proteins (2A-a) to (2A-e): (2A-a) In SEQ ID NO: 4, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; (2A-b) Amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 4 Proteins containing; (2A-c) One or more nucleotides are deleted, substituted, inserted, or attached in SEQ ID NO: 3 A protein containing an amino acid sequence encoded by the added base sequence; (2A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 3 A protein containing the amino acid sequence that is to be used; or (2A-e) A nucleic acid consisting of a base sequence complementary to the base sequence shown in Sequence ID No. 3 A protein comprising an amino acid sequence encoded by nucleic acids that hybridize under triggering conditions; or (2B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 21 to 28; (3) (3A) Gamma-glutamyl hydrolase or a variant thereof, which is any of the following proteins (3A-a) to (3A-e): (3A-a) In SEQ ID NO: 6, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; (3A-b) Amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 6 Proteins containing; (3A-c) In SEQ ID NO: 5, one or more nucleotides are deleted, substituted, inserted, or attached. A protein containing an amino acid sequence encoded by the added base sequence; (3A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 5 A protein containing the amino acid sequence that is to be used; or (3A-e) A nucleic acid consisting of a base sequence complementary to the base sequence shown in Sequence ID No. 5 A protein comprising an amino acid sequence encoded by nucleic acids that hybridize under triggering conditions; or (3B) Proteins containing the amino acid sequence shown in SEQ ID NO: 29 or 30; (4) (4A) Guanine nucleotide-binding protein subunit beta-like protein or a variant thereof, which is any of the following proteins (4A-a) to (4A-e): (4A-a) In SEQ ID NO: 8, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing a specific amino acid sequence; (4A-b) Amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 8 Proteins containing; (4A-c) In SEQ ID NO: 7, one or more nucleotides are deleted, substituted, inserted, or attached. A protein containing an amino acid sequence encoded by the added base sequence; (4A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 7 A protein containing the amino acid sequence that is to be used; or (4A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 7 A protein comprising an amino acid sequence encoded by nucleic acids that hybridize under triggering conditions; or (4B) Proteins containing the amino acid sequence shown in SEQ ID NO: 31 or 32; (5) (5A) Protein SLE3 or a variant thereof, which is any of the following proteins (5A-a) to (5A-e): (5A-a) In SEQ ID NO: 10, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing the specified amino acid sequence; (5A-b) Amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 10 Proteins containing rows; (5A-c) One or more nucleotides are deleted, substituted, inserted, or attached in SEQ ID NO: 9 A protein containing an amino acid sequence encoded by the added base sequence; (5A-d) A sequence with 70% or more identity with the sequence shown in Sequence ID No. 9 A protein containing the amino acid sequence that is to be used; or (5A-e) A nucleic acid consisting of a base sequence complementary to the base sequence shown in Sequence ID No. 9 A protein comprising an amino acid sequence encoded by nucleic acids that hybridize under triggering conditions; or (5B) Proteins containing the amino acid sequence shown in SEQ ID NO: 33 or 34; (6) (6A) Seed biotin-containing protein SBP65 or a variant thereof, which is any of the following proteins (6A-a) to (6A-e): (6A-a) In SEQ ID NO: 12, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing the specified amino acid sequence; (6A-b) Amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 12 Proteins containing rows; (6A-c) In SEQ ID NO: 11, one or more nucleotides are deleted, substituted, inserted or A protein containing an amino acid sequence encoded by an added base sequence; (6A-d) A base sequence that is 70% or more identical to the base sequence shown in Sequence ID No. 11 A protein containing the encoded amino acid sequence; or (6A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 11 A protein containing an amino acid sequence encoded by nucleic acids that hybridize under stringent conditions; or (6B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 35 to 53; (7) (7A) Ferritin-2 or its variant, the following (7A- a) Any of the proteins listed in (7A-e): (7A-a) In SEQ ID NO: 14, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing the specified amino acid sequence; (7A-b) Amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 14 Proteins containing rows; (7A-c) In SEQ ID NO: 13, one or more nucleotides are deleted, substituted, inserted or A protein containing an amino acid sequence encoded by an added base sequence; (7A-d) A base sequence that is 70% or more identical to the base sequence shown in Sequence ID No. 13 A protein containing the encoded amino acid sequence; or (7A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 13 A protein containing an amino acid sequence encoded by nucleic acids that hybridize under stringent conditions; or (7B) Proteins containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 54-61. (8) (8A) Cell division cycle protein 48 homolog or a variant thereof, which is any of the following proteins (8A-a) to (8A-e): (8A-a) In SEQ ID NO: 16, one or more amino acids are deleted, substituted, inserted, or added. Proteins containing the specified amino acid sequence; (8A-b) Amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 16 Proteins containing rows; (8A-c) In SEQ ID NO: 15, one or more nucleotides are deleted, substituted, inserted or A protein containing an amino acid sequence encoded by an added base sequence; (8A-d) A base sequence that is 70% or more identical to the base sequence shown in Sequence ID No. 15 A protein containing the encoded amino acid sequence; or (8A-e) A nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in Sequence ID No. 15 A protein containing an amino acid sequence encoded by nucleic acids that hybridize under stringent conditions; or (8B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 62-68; The diagnostic kit comprising as an antigen.

2. A diagnostic kit for soy allergy, containing at least one of the following proteins: (1α) to (55α): (1α)(1αA1)endoplasmin homolog isoform X2 or a variant thereof, comprising any of the following proteins (1αA1-a) to (1αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (1αA1-a)Sequence ID 70; (1αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 70; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (1αA1-c)Sequence ID 69; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (1αA1-d)Sequence No. 69; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (1αA1-e)SEQ ID NO: 69; (1αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 71-87; (1αA2)endoplasmin homolog isoform X2 or a variant thereof, comprising any of the following proteins (1αA2-a) to (1αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (1αA2-a)Sequence No. 89; (1αA2-b) The amino acid sequence is 70% or more identical to the amino acid sequence shown in SEQ ID NO:

89. Proteins containing anoacid sequences; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (1αA2-c)Sequence ID 88; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (1αA2-d)Sequence No. 88; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (1αA2-e)SEQ ID NO: 88; or (1αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 90-104; (2α)(2αA1)heat shock cognate protein 80 or its variants, the following One of the proteins (2αA1-a) to (2αA1-e): (2αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 106; (2αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 106; (2αA1-c) Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 105; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (2αA1-d)Sequence No. 105; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (2αA1-e)SEQ ID NO: 105; (2αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 107-120; (2αA2) heat shock protein 90-1 or its variant, the following (2αA2- a) Any of the proteins from (2αA2-e): (2αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 122; (2αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 122; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (2αA2-c)Sequence ID 121; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (2αA2-d)Sequence ID 121; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (2αA2-e)SEQ ID NO: 121; or (2αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 123-137; (2αA3) hypothetical protein GLYMA_02G302500 or its variant, which is any of the following proteins (2αA3-a) to (2αA3-e): (2αA3-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 139; (2αA3-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 139; (2αA3-c) Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in SEQ ID NO: 138; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (2αA3-d)Sequence ID 138; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in (2αA3-e)Sequence ID 138; or (2αB3) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 140-153; (4α)(4αA)embryo-specific urease isoform X1 or its variant, the following One of the proteins (4αA-a) to (4αA-e): (4αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 155; (4αA-b) Proteins containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 155; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (4αA-c)Sequence ID 154; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (4αA-d)Sequence ID 154; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (4αA-e)SEQ ID NO: 154; or (4αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 156 and 157; (5α)(5αA)alpha-1,4 glucan phosphorylase L isozyme, chloroplastic / amyloplastic or a variant thereof, which is any of the following proteins (5αA-a) to (5αA-e): (5αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 159; (5αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 159; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (5αA-c)Sequence ID 158; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (5αA-d) Sequence ID No. 158; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (5αA-e)SEQ ID NO: 158; or (5αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 160 and 161; (6α)(6αA1)aconitate hydratase 1 or its variant, wherein the following (6αA One of the proteins 1-a) to (6αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (6αA1-a)Sequence ID 163; (6αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 163; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (6αA1-c)Sequence ID 162; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (6αA1-d)Sequence ID 162; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (6αA1-e)SEQ ID NO: 162; (6αB1) A small number of amino acid sequences selected from the group consisting of SEQ ID NOs: 164-167 A protein containing one of the following; or (6αA2)aconitate hydratase 1 or its variant, the following (6αA2-a) ~ (6αA2-e) proteins: Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (6αA2-a)Sequence ID 169; (6αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 169; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (6αA2-c)Sequence ID 168; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (6αA2-d)Sequence ID 168; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (6αA2-e)SEQ ID NO: 168; or (6αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 170-172; (7α)(7αA)methionine synthase or its variant, wherein the following (7αA-a ) to (7αA-e) proteins: (7αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 174; (7αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 174; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (7αA-c)Sequence ID 173; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (7αA-d) Sequence ID No. 173; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (7αA-e)SEQ ID NO: 173; or (7αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 175-187; (8α)(8αA)transketolase, chloroplastic, or a variant thereof, comprising any of the following proteins (8αA-a) to (8αA-e): (8αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 189; (8αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 189; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (8αA-c)Sequence ID 188; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (8αA-d)Sequence ID 188; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (8αA-e)SEQ ID NO: 188; or (8αB) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 190-199; (9α)(9αA)lysine-tRNA ligase, cytoplasmic-like isoform X2 or its variant The protein is one of the following (9αA-a) to (9αA-e): (9αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 201; (9αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 201; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (9αA-c)Sequence ID 200; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (9αA-d) Sequence ID No. 200; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (9αA-e)Sequence ID 200; or (9αB) Protein containing the amino acid sequence shown in Sequence ID No. 202; (10α)(10αA1)chaperonin CPN60-2, mitochondrial or its variant , one of the following proteins (10αA1-a) to (10αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (10αA1-a)Sequence No. 204; (10αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 204; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (10αA1-c)Sequence ID 203; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (10αA1-d)Sequence No. 203; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (10αA1-e)SEQ ID NO: 203; (10αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 205-218; (10αA2) chaperonin CPN60-2, mitochondrial-like isoform X1 or its variant The protein in question is one of the following (10αA2-a) to (10αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (10αA2-a)Sequence ID 220; (10αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 220; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (10αA2-c)SEQ ID NO: 219; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (10αA2-d)SEQ ID NO: 219; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (10αA2-e)SEQ ID NO: 219; or (10αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 221-231; (11α)(11αA)polyadenylate-binding protein 8 isoform X3 or a variant thereof, which is any of the following proteins (11αA-a) to (11αA-e): (11αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 233; (11αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 233; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (11αA-c)Sequence ID 232; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (11αA-d)Sequence No. 232; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (11αA-e)SEQ ID NO: 232; or (11αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 234-237; (12α)(12αA)pyrophosphate-fructose 6-phosphate 1-phosphotransferase subunit alpha-like or a variant thereof, comprising any of the following proteins (12αA-a) to (12αA-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (12αA-a)Sequence ID 239; (12αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 239; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (12αA-c)Sequence ID 238; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (12αA-d)SEQ ID NO: 238; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (12αA-e)SEQ ID NO: 238; or (12αB) Protein containing the amino acid sequence shown in Sequence ID No. 240; (13α)(13αA1)protein disulfide isomerase-like protein precursor or mutant thereof, comprising any of the following proteins (13αA1-a) to (13αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (13αA1-a)Sequence ID 242; (13αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 242; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (13αA1-c)SEQ ID NO: 241; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (13αA1-d)SEQ ID NO: 241; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (13αA1-e)SEQ ID NO: 241; (13αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 243-252; (13αA2)protein disulfide-isomerase or a variant thereof, wherein the following (13 One of the proteins αA2-a) to (13αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (13αA2-a)Sequence No. 254; (13αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 254; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (13αA2-c)Sequence ID 253; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (13αA2-d)SEQ ID NO: 253; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (13αA2-e)SEQ ID NO: 253; or (13αB2) A small number of amino acid sequences selected from the group consisting of SEQ ID NOs: 255-278 A protein containing at least one of these proteins; (14α)(14αA1)V-type proton ATPase subunit B 2 or its variant, which is any of the following proteins (14αA1-a) to (14αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (14αA1-a)Sequence ID 280; (14αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 280; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (14αA1-c)Sequence ID 279; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (14αA1-d)Sequence No. 279; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (14αA1-e)SEQ ID NO: 279; (14αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 281-284; (14αA2)V-type proton ATPase subunit B 2 or its variant, which is any of the following proteins (14αA2-a) to (14αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (14αA2-a)Sequence No. 286; Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in (14αA2-b) Sequence ID No. 286; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (14αA2-c)SEQ ID NO: 285; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (14αA2-d)SEQ ID NO: 285; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (14αA2-e)SEQ ID NO: 285; or (14αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 287-289; (15,16α) (15,16αA1)UTP-glucose-1-phosphate uridylyltransferase or a variant thereof, which is any of the following proteins (15,16αA1-a) to (15,16αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (15,16αA1-a)Sequence ID 291; (15,16αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 291; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (15,16αA1-c)SEQ ID NO: 290; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (15,16αA1-d)Sequence No. 290; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (15,16αA1-e)SEQ ID NO: 290; (15,16αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 292-304; (15,16αA2) hypothetical protein GLYMA_02G241100 or its variant, which is any of the following proteins (15,16αA2-a) to (15,16αA2-e) ： Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (15,16αA2-a)Sequence ID 306; (15,16αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 306; Proteins comprising an amino acid sequence encoded by a nucleotide sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (15,16αA2-c)Sequence ID 305; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (15,16αA2-d)Sequence ID 305; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (15,16αA2-e)SEQ ID NO: 305; or (15,16αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 307-318; (17α)(17αA1)guanosine nucleotide diphosphate dissociation inhibitor 2 or a variant thereof, any of the following (17αA1-a) to (17αA1-e) Proteins: Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (17αA1-a)Sequence ID 320; (17αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 320; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (17αA1-c)Sequence ID 319; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (17αA1-d)Sequence No. 319; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (17αA1-e)Sequence ID 319; (17αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 321-325; (17αA2)rab GDP dissociation inhibitor alpha-like or its variant, One of the following proteins (17αA2-a) to (17αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (17αA2-a)Sequence ID 327; (17αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 327; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (17αA2-c)SEQ ID NO: 326; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (17αA2-d)SEQ ID NO: 326; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (17αA2-e)SEQ ID NO: 326; or (17αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 328-332; (18α)(18αA) hypothetical protein GLYMA_10G028300 or its variant, which is any of the following proteins (18αA-a) to (18αA-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (18αA-a)Sequence No. 334; Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in (18αA-b) Sequence ID No. 334; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (18αA-c)Sequence ID 333; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (18αA-d)Sequence No. 333; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (18αA-e)SEQ ID NO: 333; or (18αB) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 335-339; (19α)(19αA)sucrose binding protein homolog S-64 or a variant thereof, comprising any of the following proteins (19αA-a) to (19αA-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (19αA-a)Sequence ID 341; (19αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 341; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (19αA-c)Sequence ID 340; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (19αA-d)Sequence No. 340; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (19αA-e)Sequence ID 340; or (19αB) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 342-346; (25α)(25αA1)succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial or its variant, of which (25αA1-a) to (25αA1-e) below One of the proteins: Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (25αA1-a)Sequence ID 348; (25αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 348; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (25αA1-c)Sequence ID 347; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (25αA1-d)Sequence ID 347; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (25αA1-e)SEQ ID NO: 347; (25αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 349-356; (25αA2) succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial or This is a variant of the following proteins: (25αA2-a) to (25αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (25αA2-a)Sequence ID 358; (25αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 358; In (25αA2-c) Sequence ID No. 357, one or more nucleotides are deleted or substituted. , a protein containing an amino acid sequence encoded by an inserted or added nucleotide sequence; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (25αA2-d)Sequence ID 357; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in (25αA2-e)SEQ ID NO: 357; or (25αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 359-365; (26α)(26αA1)phosphoglycerate kinase, cytosolic, or a variant thereof, comprising any of the following proteins (26αA1-a) to (26αA1-e): (26αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 367; (26αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 367; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (26αA1-c)Sequence ID 366; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (26αA1-d)Sequence ID 366; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (26αA1-e)SEQ ID NO: 366; (26αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 368-382; (26αA2)phosphoglycerate kinase, cytosolic, or a variant thereof, comprising any of the following proteins (26αA2-a) to (26αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (26αA2-a)Sequence ID 384; Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in (26αA2-b) Sequence ID No. 384; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (26αA2-c)Sequence ID 383; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (26αA2-d)SEQ ID NO: 383; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (26αA2-e)SEQ ID NO: 383; (26αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 385-392; (26αA3)phosphoglycerate kinase, cytosolic, or a variant thereof, comprising any of the following proteins (26αA3-a) to (26αA3-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (26αA3-a)Sequence ID 394; (26αA3-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 394; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (26αA3-c)Sequence ID 393; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (26αA3-d)Sequence ID 393; or (26αA3-e) The nucleic acid encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a base sequence complementary to the base sequence shown in Sequence ID No.

393. Proteins containing anoacid sequences; or (26αB3) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 395-409; (27α)(27αA1)alcohol dehydrogenase 1 or its variant, the following ( One of the proteins 27αA1-a) to (27αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (27αA1-a)Sequence ID 411; (27αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 411; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (27αA1-c)Sequence ID 410; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (27αA1-d)Sequence No. 410; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (27αA1-e)SEQ ID NO: 410; (27αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 412-416; (27αA2)alcohol dehydrogenase 1 or its variant, wherein the following (27αA One of the proteins 2-a) to (27αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (27αA2-a)Sequence ID 418; (27αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 418; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (27αA2-c)Sequence ID 417; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (27αA2-d)SEQ ID NO: 417; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (27αA2-e)SEQ ID NO: 417; (27αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 419 and 420; (27αA3)alcohol dehydrogenase 1-like or a variant thereof, comprising any of the following proteins (27αA3-a) to (27αA3-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (27αA3-a)Sequence ID 422; (27αA3-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 422; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (27αA3-c)SEQ ID NO: 421; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (27αA3-d)SEQ ID NO: 421; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (27αA3-e)SEQ ID NO: 421; or (27αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 423-427; (28α)(28αA) 35 kDa seed maturation protein isoform X1 or its mutant The protein present is one of the following (28αA-a) to (28αA-e): (28αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 429; (28αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 429; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (28αA-c)Sequence ID 428; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (28αA-d)SEQ ID NO: 428; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (28αA-e)SEQ ID NO: 428; or (28αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 430-433; (29-33α)(29-33αA1)glyceraldehyde-3-phosphate dehydrogenase isoform X1 or its variants, the following (29-33αA1-a) to (29-33αA 1-e) Any of the proteins: Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (29-33αA1-a)Sequence ID 435; (29-33αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 435; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (29-33αA1-c)Sequence ID 434; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (29-33αA1-d)Sequence ID 434; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (29-33αA1-e)SEQ ID NO: 434; (29-33αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 436-439; (29-33αA2) glyceraldehyde-3-phosphate dehydrogenase, cytosolic or its A variant of the following proteins (29-33αA2-a) to (29-33αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (29-33αA2-a)Sequence ID 441; Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in (29-33αA2-b)Sequence ID 441; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (29-33αA2-c)SEQ ID NO: 440; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (29-33αA2-d)SEQ ID NO: 440; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (29-33αA2-e)SEQ ID NO: 440; (29-33αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 442-445; (29-33αA3)uncharacterized protein LOC100782924 or its variant, which is any of the following proteins (29-33αA3-a) to (29-33αA3-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (29-33αA3-a)Sequence ID 447; Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in (29-33αA3-b) Sequence ID No. 447; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (29-33αA3-c)SEQ ID NO: 446; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (29-33αA3-d)Sequence ID 446; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (29-33αA3-e)SEQ ID NO: 446; or (29-33αB3) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 448-451; (34α)(34αA1)bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1-like or a variant thereof, comprising any of the following proteins (34αA1-a) to (34αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (34αA1-a)Sequence ID 453; (34αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 453; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (34αA1-c)Sequence ID 452; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (34αA1-d)Sequence ID 452; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (34αA1-e)SEQ ID NO: 452; (34αB1) A protein containing the amino acid sequence shown in Sequence ID No. 454; (34αA2)uncharacterized protein LOC100803483 or its variant, which is any of the following proteins (34αA2-a) to (34αA2-e): (34αA2-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 456; (34αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 456; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (34αA2-c)Sequence ID 455; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (34αA2-d)Sequence ID 455; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (34αA2-e)SEQ ID NO: 455; or (34αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 457 and 458; (35α)(35αA1)elongation factor 1-alpha or its variants, the following One of the proteins (35αA1-a) to (35αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (35αA1-a)Sequence ID 460; (35αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 460; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (35αA1-c)Sequence ID 459; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (35αA1-d)Sequence ID 459; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (35αA1-e)SEQ ID NO: 459; (35αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 461-466; (35αA2) elongation factor 1-alpha or its variant, wherein the following (35α One of the proteins A2-a) to (35αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (35αA2-a)Sequence ID 468; (35αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 468; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (35αA2-c)Sequence ID 467; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (35αA2-d)Sequence ID 467; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (35αA2-e)SEQ ID NO: 467; (35αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 469-471; (35αA3)elongation factor-1A isoform X1 or its variant, the following (3 One of the proteins 5αA3-a) to (35αA3-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (35αA3-a)Sequence ID 473; (35αA3-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 473; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (35αA3-c)SEQ ID NO: 472; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (35αA3-d)Sequence ID 472; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in (35αA3-e)SEQ ID NO: 472; or (35αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 474-477; (36,37α)(36,37αA1) seed maturation protein PM34 or its variant, which is any of the following proteins (36,37αA1-a) to (36,37αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (36,37αA1-a)Sequence ID 479; (36,37αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in SEQ ID NO: 479; Proteins comprising an amino acid sequence encoded by a nucleotide sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (36,37αA1-c)Sequence ID 478; (36,37αA1-d) The base sequence shown in SEQ ID NO: 478 has a 70% or greater identity. A protein containing an amino acid sequence encoded by the base sequence; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (36,37αA1-e)SEQ ID NO: 478; (36,37αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 480-483; (36,37αA2)uncharacterized protein LOC100809384 or its variant, which is any of the following proteins (36,37αA2-a) to (36,37αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (36,37αA2-a)Sequence ID 485; (36,37αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 485; Proteins containing amino acid sequences encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (36,37αA2-c)Sequence ID 484; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (36,37αA2-d)Sequence ID 484; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (36,37αA2-e)Sequence ID 484; (36,37αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 486 and 487; (36,37αA3) hypothetical protein GLYMA_10G155300 or its variant, which is any of the following proteins (36,37αA3-a) to (36,37αA3-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (36,37αA3-a)Sequence ID 489; Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in (36,37αA3-b) Sequence ID No. 489; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (36,37αA3-c)Sequence ID 488; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (36,37αA3-d)Sequence ID 488; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (36,37αA3-e)SEQ ID NO: 488; or (36,37αB3) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 490-493; (38α)(38αA) seed biotin-containing protein SBP65-like isoform X1 or its variant, which is any of the following proteins (38αA-a) to (38αA-e): (38αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 495; (38αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 495; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (38αA-c)Sequence ID 494; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (38αA-d)Sequence ID 494; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in (38αA-e)Sequence ID 494; or (38αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 496-513; (39α)(39αA)ATP synthase subunit O, mitochondrial-like or a variant thereof, comprising any of the following proteins (39αA-a) to (39αA-e): (39αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 515; (39αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 515; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (39αA-c)Sequence ID 514; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (39αA-d)Sequence ID 514; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (39αA-e)Sequence ID 514; or (39αB) Protein containing the amino acid sequence shown in Sequence ID No. 516; (40,56α)(40,56αA)P24 oleosin isoform A or its variant, One of the following proteins (40,56αA-a) to (40,56αA-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (40,56αA-a)Sequence ID 518; (40,56αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 518; Proteins comprising an amino acid sequence encoded by a nucleotide sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (40,56αA-c)SEQ ID NO: 517; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (40,56αA-d)Sequence ID 517; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (40,56αA-e)SEQ ID NO: 517; or (40,56αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 519 and 520; (41α)(41αA)uncharacterized protein At3g49720-like isoform X2 or the A mutant, which is one of the following proteins (41αA-a) to (41αA-e): (41αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 522; (41αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 522; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (41αA-c)Sequence ID 521; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (41αA-d)Sequence ID 521; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (41αA-e)SEQ ID NO: 521; or (41αB) Protein containing the amino acid sequence shown in Sequence ID No. 523; (42,43α)(42,43αA1)superoxide dismutase [Mn], mitochondrial or its mutant, the following (42,43αA1-a) to (42,43αA1-e) One of the proteins: Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (42,43αA1-a)Sequence ID 525; (42,43αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 525; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (42,43αA1-c)Sequence ID 524; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (42,43αA1-d)Sequence ID 524; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (42,43αA1-e)SEQ ID NO: 524; (42,43αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 526-531; (42,43αA2) hypothetical protein GLYMA_04G221300 or its variant, which is any of the following proteins (42,43αA2-a) to (42,43αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (42,43αA2-a)Sequence ID 533; Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in (42,43αA2-b) Sequence ID No. 533; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (42,43αA2-c)Sequence ID 532; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (42,43αA2-d)Sequence ID 532; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (42,43αA2-e)Sequence ID 532; or (42,43αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 534-539; (45α)(45αA1)uncharacterized protein LOC100499771 or its variant, which is any of the following proteins (45αA1-a) to (45αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (45αA1-a)Sequence ID 541; (45αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 541; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (45αA1-c)SEQ ID NO: 540; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (45αA1-d)Sequence ID 540; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (45αA1-e)SEQ ID NO: 540; (45αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 542-545; (45αA2) hypothetical protein GLYMA_09G192800 or its variant, which is any of the following proteins (45αA2-a) to (45αA2-e): (45αA2-a) In sequence number 547, one or more amino acids are deleted, substituted, or inserted. Proteins containing added or modified amino acid sequences; (45αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 547; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (45αA2-c)SEQ ID NO: 546; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (45αA2-d)SEQ ID NO: 546; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (45αA2-e)SEQ ID NO: 546; or (45αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 548-551; (46α)(46αA) seed maturation protein PM22 or its variant, which is any of the following proteins (46αA-a) to (46αA-e): (46αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 553; (46αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 553; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (46αA-c)Sequence ID 552; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (46αA-d)SEQ ID NO: 552; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (46αA-e)SEQ ID NO: 552; or (46αB) Protein containing the amino acid sequence shown in Sequence ID No. 554; (47α)(47αA1) eukaryotic translation initiation factor 5A-2 or its variant A variant of any of the following proteins (47αA1-a) to (47αA1-e): (47αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 556; (47αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 556; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (47αA1-c)SEQ ID NO: 555; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (47αA1-d)SEQ ID NO: 555; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (47αA1-e)SEQ ID NO: 555; (47αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 557 and 558; (47αA2)uncharacterized protein LOC100305510 or its variant, which is any of the following proteins (47αA2-a) to (47αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (47αA2-a)Sequence ID 560; (47αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in SEQ ID NO: 560; Proteins containing an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (47αA2-c)SEQ ID NO: 559; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (47αA2-d)Sequence ID 559; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (47αA2-e)SEQ ID NO: 559; or (47αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 561-563; (48α)(48αA)uncharacterized protein LOC100306570 or its variant, which is any of the following proteins (48αA-a) to (48αA-e): (48αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 565; (48αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 565; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (48αA-c)Sequence ID 564; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (48αA-d)Sequence ID 564; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (48αA-e)SEQ ID NO: 564; or (48αB) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 566-570; (50α)(50αA)uncharacterized protein LOC100813859 or its variant, which is any of the following proteins (50αA-a) to (50αA-e): (50αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 572; (50αA-b) Proteins containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 572; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (50αA-c)Sequence ID 571; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (50αA-d)Sequence ID 571; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (50αA-e)Sequence ID 571; or (50αB) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 573 to 576; (51α)(51αA)14-3-3-like protein or a variant thereof, wherein the following (51α Protein A-a) to (51αA-e): (51αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 578; (51αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 578; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (51αA-c)Sequence ID 577; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (51αA-d)Sequence ID 577; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (51αA-e)Sequence ID 577; or (51αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 579 to 582; (52α)(52αA)probable fructokinase-4 or its variant, the following (5 One of the proteins 2αA-a) to (52αA-e): (52αA-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 584; (52αA-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 584; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (52αA-c)Sequence ID 583; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (52αA-d)Sequence ID 583; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (52αA-e)SEQ ID NO: 583; or (52αB) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 585-588; (53α)(53αA1)triosephosphate isomerase isoform X1 or a variant thereof, comprising any of the following proteins (53αA1-a) to (53αA1-e): (53αA1-a) Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in SEQ ID NO: 590; (53αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 590; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (53αA1-c)Sequence ID 589; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (53αA1-d)Sequence ID 589; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (53αA1-e)SEQ ID NO: 589; (53αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 591-597; (53αA2)triosephosphate isomerase, cytosolic, or a variant thereof, comprising any of the following proteins (53αA2-a) to (53αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (53αA2-a)Sequence ID 599; (53αA2-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 599; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (53αA2-c)Sequence ID 598; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (53αA2-d)Sequence ID 598; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (53αA2-e)SEQ ID NO: 598; or (53αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 600-604; (54α) (54αA1) superoxide dismutase [Fe], chloroplastic isoform X1 or The mutant is one of the following proteins (54αA1-a) to (54αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (54αA1-a)Sequence ID 606; (54αA1-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 606; Proteins comprising an amino acid sequence encoded by a nucleotide sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (53αA1-c)Sequence ID 605; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (54αA1-d)Sequence ID 605; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (54αA1-e)SEQ ID NO: 605; (54αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 607-611; (54αA2)iron-superoxide dismutase or a variant thereof, wherein the following (54αA 2-a) to (54αA2-e) any of the proteins: Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (54αA2-a)Sequence ID 613; (54αA2-b) Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in Sequence ID No. 613; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (54αA2-c)SEQ ID NO: 612; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (54αA2-d)SEQ ID NO: 612; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (54αA2-e)SEQ ID NO: 612; or (54αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 614-616; or (55α)(55αA1) 51 kDa seed maturation protein precursor or its variant, which is any of the following proteins (55αA1-a) to (55αA1-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (55αA1-a)Sequence ID 618; (55αA1-b) A protein containing an amino acid sequence that is 70% or more identical to the amino acid sequence shown in Sequence ID No. 618; Proteins comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides are deleted, substituted, inserted, or added in (55αA1-c)SEQ ID NO: 617; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (55αA1-d)SEQ ID NO: 617; or A protein containing an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (55αA1-e)SEQ ID NO: 617; (55αB1) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 619-635; (55αA2) Lea protein precursor or its variant, the following (55αA2- a) Any of the proteins from (55αA2-e): Proteins containing an amino acid sequence in which one or more amino acids are deleted, substituted, inserted, or added in (55αA2-a)Sequence ID 637; Proteins containing amino acid sequences that are 70% or more identical to the amino acid sequence shown in (55αA2-b) Sequence ID No. 637; (55αA2-c) Sequence ID 636 has one or more nucleotides deleted or substituted. , a protein containing an amino acid sequence encoded by an inserted or added nucleotide sequence; A protein containing an amino acid sequence encoded by a nucleotide sequence that is 70% or more identical to the nucleotide sequence shown in (55αA2-d)SEQ ID NO: 636; or A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in (55αA2-e)SEQ ID NO: 636; or (55αB2) A protein containing at least one amino acid sequence selected from the group consisting of SEQ ID NOs. 638-649; The diagnostic kit comprising as an antigen.

3. A diagnostic composition for soy allergy, comprising at least one of the proteins specified as (1) to (8) in claim 1, or at least one of the proteins specified as (1α) to (55α) in claim 2, as an antigen.

4. A method for providing indicators for diagnosing a target soy allergy, comprising the following steps: (i) The sample obtained from the subject is brought into contact with the antigen, where the sample is a solution containing Ig antibody; (ii) Detect the binding of IgE antibodies to the antigen in the sample obtained from the subject; (iii) If binding of the target IgE antibody to the antigen is detected, it provides an indicator that the subject has a soy allergy; The method comprising, wherein the antigen is at least one of the proteins specified as (1) to (8) in claim 1, or at least one of the proteins specified as (1α) to (55α) in claim 2.

5. A pharmaceutical composition comprising at least one of the proteins specified as (1) to (8) in claim 1, or at least one of the proteins specified as (1α) to (55α) in claim 2.

6. A pharmaceutical composition according to claim 5 for treating soy allergy.

7. Soybeans or soybean products characterized in that an antigen is removed or reduced, wherein the antigen is at least one of the proteins specified as (1) to (8) in claim 1, or at least one of the proteins specified as (1α) to (55α) in claim 2.

8. A tester for determining the presence or absence of soybean antigen in a target object, characterized by comprising an antibody that binds to at least one of the proteins specified as (1) to (8) in claim 1, or at least one of the proteins specified as (1α) to (55α) in claim 2.

9. An antigen derived from soybeans, wherein the antigen is at least one of the proteins specified as (1) to (8) in claim 1 or at least one of the proteins specified as (1α) to (55α) in claim 2, and is the cause of an allergy to soybeans.

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