Measuring device and measuring method

Abstract

The present invention provides a measuring device and a measuring method that enable the maintenance of solution quality and facilitate simple operations. [Solution] The measuring device 1 includes a holder for supporting a first container C1 containing a first solution S1, a heater 25 for thawing the first solution S1, a first adding unit 41 for adding a second solution S2 to the first solution S1, a second adding unit 42 for adding a biological sample S3 to the first solution S1, a first adjusting unit 21 for adjusting the position of adding the second solution S2 relative to the liquid surface T of the first solution S1, and an optical unit 27 for irradiating the thawed mixture of the first solution S1, the second solution S2, and the biological sample S3 with excitation light L1 and detecting detection light L2.

Description

Technical Field

[0001] The present invention relates to a measuring device and a measuring method.

Background Art

[0002] Conventionally, techniques for measuring the characteristics of a biological sample have been known (see, for example, Patent Document 1). In such techniques, after a biological sample collected from a subject (for example, a human body) is added to a prepared solution prepared by introducing a plurality of reagents into a container, the light generated in the storage space of the container is measured, whereby the characteristics of the biological sample may be measured.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] In the above-described techniques, at least one of the plurality of reagents may be liable to deteriorate at room temperature. In that case, it is desirable to perform operations such as the preparation of the prepared solution and the introduction of the prepared solution into the container immediately before the measurement of light and in the vicinity of the subject. However, if these operations are performed in the vicinity of the subject, the operations may become complicated.

[0005] An object of the present invention is to provide a measuring device and a measuring method that enable simple operations.

Means for Solving the Problems

[0006] The measuring device according to the present invention is a measuring device used for measuring the properties of a biological sample, comprising: a support part for supporting a first container containing a first solution which is frozen and contains an indicator which reacts with components generated from the biological sample; a heater for heating the first solution in the first container when the support part is supporting the first container; a first addition part for adding a second solution which contains an stimulant which activates the function of the biological sample to the first solution in the first container when the support part is supporting the first container; a second addition part for adding the biological sample to the first solution in the first container when the support part is supporting the first container; and a heater for heating the liquid surface of the first solution in the first container when the support part is supporting the first container The measuring device comprises: a first adjustment unit for adjusting the addition position of the second solution; and an optical unit for irradiating the mixture of the thawed first solution, the second solution added to the first solution, and the biological sample with excitation light, and detecting detection light including fluorescence generated in the mixture as a result of the irradiation with excitation light, wherein the first addition unit is fitted with a second container containing the second solution and has a first nozzle for dispensing the second solution from the second container into the first solution, and the first adjustment unit adjusts the addition position of the second solution relative to the liquid surface in the height direction by moving the first nozzle along the height direction intersecting the liquid surface.

[0007] The measuring device according to the present invention is [2] "a measuring device used for measuring the properties of a biological sample, comprising: a support part for supporting a first container containing a frozen first solution for diluting the biological sample; a heater for heating the first solution in the first container in a supported state in which the support part supports the first container; a first additive part for adding a second solution containing a stimulant for activating the function of the biological sample to the first solution in the first container in the supported state; a second additive part for adding an indicator that reacts with the biological sample and components generated from the biological sample to the first solution in the first container in the supported state; and a heater for heating the liquid surface of the first solution in the first container in the supported state The measuring device comprises: a first adjustment unit for adjusting the addition position of the second solution; and an optical unit for irradiating a mixture of the thawed first solution, the second solution added to the first solution, the biological sample, and the indicator with excitation light, and detecting detection light including fluorescence generated in the mixture by the irradiation of the excitation light, wherein the first addition unit is fitted with a second container containing the second solution and has a first nozzle for dispensing the second solution from the second container into the first solution, and the first adjustment unit adjusts the addition position of the second solution relative to the liquid surface in the height direction by moving the first nozzle along the height direction intersecting the liquid surface.

[0008] The measurement method according to the present invention is described as follows: [9] "A measurement method for measuring the properties of a biological sample using a measuring device, comprising: a first step of supporting a first container containing a first solution which is frozen and contains an indicator that reacts with components generated from the biological sample, on a support part of the measuring device; a second step of thawing the first solution in the first container with a heater of the measuring device after the first step; a third step of attaching a second container which contains a second solution which is contained and contains a stimulant that activates the function of the biological sample, to a first nozzle of the measuring device; a fourth step of adjusting the position of adding the second solution relative to the liquid surface of the first solution in the first container after the second and third steps; and after the fourth step, The measurement method comprises: a fifth step of adding the second solution to the first solution; a sixth step of adding the biological sample to the first solution in the first container after the second step; and a seventh step of irradiating the mixture of the first solution, the biological sample added to the first solution, and the second solution with excitation light using the optical unit of the measuring device, and detecting detection light including fluorescence generated in the mixture as a result of the irradiation with the excitation light, wherein in the fourth step, the first adjustment unit of the measuring device adjusts the addition position of the second solution to the liquid surface in the height direction by moving the first nozzle along the height direction intersecting the liquid surface of the first solution.

[0009] The measurement method according to the present invention is described as follows:

[10] "A measurement method for measuring the properties of a biological sample using a measuring device, comprising: a first step of supporting a first container containing a frozen first solution for diluting the biological sample on a support part of the measuring device; a second step of thawing the first solution in the first container with a heater of the measuring device after the first step; a third step of attaching a second container containing a second solution containing a stimulant for activating the function of the biological sample to a first nozzle of the measuring device; a fourth step of adjusting the addition position of the second solution relative to the liquid surface of the first solution in the first container after the second and third steps; and a fifth step of adding the second solution to the first solution in the first container after the fourth step." The measurement method comprises the following steps: a second step, a sixth step after the second step, adding the biological sample and an indicator that reacts with components generated from the biological sample to the first solution in the first container; and a seventh step after the fifth and sixth steps, using the optical unit of the measuring device, irradiating the first solution and a mixture of the biological sample added to the first solution, the indicator, and the second solution with excitation light, and detecting detection light including fluorescence generated in the mixture as a result of the irradiation with the excitation light, wherein in the fourth step, the first adjustment unit of the measuring device adjusts the addition position of the second solution relative to the liquid surface in the height direction by moving the first nozzle along the height direction intersecting the liquid surface of the first solution.

[0010] In this measuring device and method, a first container containing a frozen first solution is supported by a support unit, and in this state, the first solution can be heated by a heater. This allows the first solution to be thawed near the subject (in the measuring device) and then the biological sample taken from the subject can be added to the first solution. Therefore, not only is the quality of the first solution maintained, but tasks such as preparing the first solution and introducing it into the first container become unnecessary near the subject. Thus, this measuring device and method maintains the quality of the first solution and simplifies the process of measuring the characteristics of the biological sample. Furthermore, in this measuring device and method, a first adjustment unit moves a first nozzle to which a second container is attached along a height direction intersecting the liquid surface of the first solution, thereby adjusting the addition position of the second solution relative to the liquid surface of the first solution in that height direction. This makes it possible to add the second solution to the first solution at an appropriate addition position relative to the liquid surface of the first solution.

[0011] Here, according to the inventor's findings, when adding the second solution to the first solution, if the outlet of the second solution in the second container is far from the liquid surface of the first solution, it may not be possible to completely discharge the second solution from the second container, and a large amount of the second solution discharged from the outlet of the second container will splash onto the inner wall surface of the first container. On the other hand, if the outlet of the second solution in the second container is located within the first solution, and the second solution is discharged from the second container, the liquid surface of the first solution will be greatly disturbed, resulting in greater disturbance of the detection signal of the detected light in the optical unit. Therefore, there is a need to suppress the residue of the second solution, which may be highly concentrated and in small quantities, in the second container, and to suppress disturbance of the detection signal.

[0012] Therefore, the measuring device according to the present invention may also be [3] "the measuring device according to [1] or [2] above, wherein the first adjustment unit moves the first nozzle along the height direction such that when the second solution is added to the first solution, the distance of the outlet of the second solution in the second container from the liquid surface in the height direction is 1 mm or less in the range in which the outlet does not come into contact with the liquid surface."

[0013] Furthermore, the measurement method according to the present invention may also be

[11] "the measurement method according to [9] or

[10] above, wherein in the fourth step, when the second solution is added to the first solution, the first adjustment unit of the measuring device moves the first nozzle along the height direction so that the distance from the liquid surface in the height direction of the outlet of the second solution in the second container is 1 mm or less in the range in which the outlet does not come into contact with the liquid surface."

[0014] In this case, the distance of the outlet for the second solution in the second container from the liquid surface of the first solution is adjusted to be 1 mm or less, within the range where the outlet does not come into contact with the liquid surface of the first solution. Discharging the second solution from the second container at a distance of 1 mm or less from the liquid surface of the first solution in this way suppresses the residue of the second solution remaining in the second container. It also suppresses the amount of second solution that splashes onto the inner wall surface of the first container after being discharged from the second container. Furthermore, by ensuring that the outlet for the second solution in the second container does not come into contact with the first solution, it is possible to suppress significant disturbance of the liquid surface of the first solution due to the addition of the second solution, thereby suppressing disturbance of the detection signal.

[0015] As described above, in this invention, the first solution is thawed within the measuring device, and the mixture containing the thawed first solution is subjected to optical measurement. In such a case, condensation may form on the surface of the first container during the thawing of the first solution, and this condensation may affect the optical measurement (for example, by causing scattering of excitation light and detection light). The effect of this condensation lasts for a long time, and if measurement is to be performed only after the condensation has disappeared, it will take a long time. Therefore, there is a need to quickly reduce the effect of condensation on the surface of the first container that occurs during the thawing of the first solution and shorten the measurement time.

[0016] Therefore, the measuring device according to the present invention may also be [4] "a measuring device according to any one of [1] to [3] above, comprising a blower for supplying air to the first container in the supported state, the first container including a light-transmitting section that transmits the excitation light and the detection light, and the blower supplying the air to the surface of the light-transmitting section in the supported state."

[0017] Furthermore, the measurement method according to the present invention may also be

[12] "the measurement method according to any one of [9] to

[11] above, wherein in the second step, when thawing the first solution, air is supplied to the surface of the light-transmitting portion in the first container that transmits the excitation light and the detection light using the blower of the measuring device."

[0018] In this case, by supplying air to the surface of the excitation light and detection light transmission area in the first container using a blower, it is possible to suppress condensation on the first container when the first solution is thawed, or to quickly remove any condensation that does occur on the first container. Therefore, the effect of condensation on the surface of the light transmission area in the first container can be quickly reduced, and the measurement time can be shortened.

[0019] The measuring apparatus according to the present invention may also be [5] "the measuring apparatus according to any one of [1] to [4] above, wherein the optical unit comprises an optical filter for selectively transmitting the detection light incident at an incident angle of a predetermined angle or less from the detection light from the mixture, a lens for focusing the detection light that has passed through the optical filter, and a detector for detecting the detection light focused by the lens." In this case, components affected by scattering in the detection light can be removed by the optical filter. Furthermore, even if the amount of light in the detection light decreases due to the removal of some components by the optical filter, the effect of this decrease in light amount on the detection result can be suppressed by focusing the detection light with a lens downstream of the optical filter.

[0020] The measuring device according to the present invention may be the one described in any of [1] to [5] below: "[6] In the support state, a second adjustment unit for adjusting the addition position of the biological sample to the first solution in the first container is provided. The second addition unit has a third container containing the biological sample attached thereto, and a second nozzle for导出 the biological sample from the third container to the first container. The second adjustment unit adjusts the addition position of the biological sample in the height direction by moving the second nozzle along the height direction."

[0021] Further, the measuring method according to the present invention may be the one described in any of [9] to

[12] below: "

[13] Before the sixth step, an eighth step of attaching a third container containing the biological sample to the second nozzle of the measuring device, and a ninth step of adjusting the addition position of the biological sample to the first solution in the first container after the eighth step and before the sixth step. In the ninth step, the second adjustment unit of the measuring device adjusts the addition position of the biological sample to the first solution in the height direction by moving the second nozzle along the height direction."

[0022] In this case, it becomes possible to add the biological sample to the first solution at an appropriate addition position with respect to the first solution.

[0023] The measuring device according to the present invention may be the one described in [6] below: "[7] The second adjustment unit moves the second nozzle along the height direction so that the outlet of the biological sample in the third container is located within the first solution."

[0024] The measuring method according to the present invention may be the one described in

[13] below: "

[14] In the ninth step, the second adjustment unit of the measuring device moves the second nozzle along the height direction so that the outlet of the biological sample in the third container is located within the first solution."

[0025] In this case, inside the first solution, it becomes possible to discharge the biological sample from the third container.

[0026] The measuring device according to the present invention may be the one described in [8] "when adding the biological sample to the first solution, the second adding part repeatedly performs the derivation of the biological sample from the third container by the second nozzle and the introduction of the first solution into the third container by the second nozzle, as described in the above [7]".

[0027] The measuring method according to the present invention may be the one described in

[15] "in the sixth step, when adding the biological sample to the first solution, the second nozzle repeatedly performs the derivation of the biological sample from the third container and the introduction of the first solution into the third container, as described in the above

[14] ".

[0028] In this case, by performing pipetting in the third container with the second nozzle, it becomes possible to surely add the biological sample in the third container to the first solution.

Effect of the Invention

[0029] According to the present invention, it is possible to provide a measuring device and a measuring method that enable simple operations.

Brief Description of the Drawings

[0030] [Figure 1] FIG. 1 is a schematic front view showing the measuring device according to the present embodiment. [Figure 2] FIG. 2 is a schematic side view of the measuring device shown in FIG. 1. [Figure 3] FIG. 3 is a schematic front view showing an enlarged view of the measurement unit shown in FIGS. 1 and 2. [Figure 4] FIG. 4 is a schematic diagram showing the configuration of the optical unit shown in FIG. 2. [Figure 5] FIG. 5 is a schematic front view showing a state of adjusting the addition position of the second solution. [Figure 6]Figure 6 is a flowchart showing each step corresponding to the preparation stage of the measurement method according to this embodiment. [Figure 7] Figure 7 is a flowchart showing each step of the measurement method according to this embodiment. [Modes for carrying out the invention]

[0031] Embodiments of the present invention will be described in detail below with reference to the drawings. In each drawing, the same or corresponding parts are denoted by the same reference numerals, and redundant explanations may be omitted.

[0032] Figure 1 is a schematic front view showing the measuring device according to this embodiment. Figure 2 is a schematic side view of the measuring device shown in Figure 1. Figure 3 is a schematic front view showing an enlarged view of the measuring unit shown in Figures 1 and 2. The measuring device 1 shown in Figures 1 to 3 is used to measure the properties of a biological sample. The biological sample is, for example, the blood (e.g., whole blood) of a subject (living organism). The subject is, for example, a human body. In this embodiment, the subject is a person undergoing a health checkup. The biological sample contains, for example, white blood cells. The properties of the biological sample are, for example, the activity of white blood cells. In this embodiment, the properties of the biological sample are the activity of neutrophil cells.

[0033] Neutrophils are a type of white blood cell. Their main role is to prevent infection by phagocytosing and killing bacteria and fungi that enter the body. Neutrophils engulf bacteria and other microorganisms within their neutrophil plasma membrane, forming a phagocystos. When the phagocystos fuses with granules, the granule contents are released into the phagocystos. The NADPH oxidase system formed on the cell membrane (phagocytic membrane) generates reactive oxygen species (superoxide, hydrogen peroxide), which kill bacteria and other microorganisms. Furthermore, the enzymatic reaction of myeloperoxidase (EC number 1.11.2.2) contained in the granule contents produces hydrogen peroxide (H2O2) and chloride ions (Cl). -Hypochlorous acid (HOCl) (or its halogen equivalent) is produced from ), and this hypochlorous acid kills bacteria and other microorganisms. Therefore, myeloperoxidase activity or superoxide production activity is used as an indicator for evaluating the activity of neutrophil cells. The measuring device 1 of this embodiment is used to measure myeloperoxidase activity or superoxide production activity.

[0034] The measuring device 1 comprises a measurement unit 10, a display unit 30, and an output unit 50. The measurement unit 10 measures the characteristics of a biological sample as described above. The display unit 30 is, for example, a display and can display the measurement results from the measurement unit 10. The display unit 30 is also, for example, a touch panel and can also function as an input receiving unit that receives input from the user. The output unit 50 is, for example, a printer and can output the measurement results from the measurement unit 10 by printing. In other words, in the measuring device 1, the display unit 30 and the output unit 50 constitute an input / output unit that receives input related to the measurement in the measurement unit 10 (for example, sample name and measurement conditions, etc.) and outputs the measurement results from the measurement unit 10.

[0035] The measurement unit 10 has a structure for supporting multiple containers that hold biological samples and various solutions. The containers supported by the measurement unit 10 are the first container C1, the second container C2, and the third container C3. The first container C1 contains the first solution S1 inside. The first solution S1 is a mixture of multiple reagents. The first solution S1 includes, for example, at least one of physiological saline and buffer solution, and a fluorescent indicator. The first solution S1 has the function of diluting the biological sample. The fluorescent indicator reacts with components (HOCl) generated from the biological sample. The fluorescent indicator is, for example, aminophenyl fluorescein (APF). A commercially available fluorescent indicator may be used. Note that the first solution S1 does not have to contain a fluorescent indicator. Hereinafter, the case in which the first solution S1 does not contain a fluorescent indicator will be referred to as the "first modified example". Furthermore, the fluorescent indicator may react with reactive oxygen species, such as superoxide and nitric oxide, which are components generated from the biological sample. Examples of fluorescent indicators include DCFH-DA, BES-So, and DAF-2.

[0036] The first solution S1 is contained in the first container C1 in a frozen state. The first solution S1 may be completely frozen or partially frozen. The temperature of the first solution S1 is below the freezing point of any of the reagents contained in the first solution S1. That is, the temperature of the first solution S1 may be the same as the freezing point of any of the reagents contained in the first solution S1, or it may be lower than the freezing point of any of the reagents contained in the first solution S1.

[0037] In this embodiment, the temperature of the first solution S1 is below the freezing point of the reagent with the largest volume among the multiple reagents contained in the first solution S1. The temperature of the first solution S1 may also be below the freezing point of the reagent with the lowest freezing point among the multiple reagents contained in the first solution S1. In this embodiment, the temperature of the first solution S1 is below the freezing point of the buffer solution contained in the first solution S1. The temperature of the first solution S1 may also be below the freezing point of the fluorescent indicator contained in the first solution S1. In this embodiment, the overall temperature of the first container C1 is below the freezing point of any of the reagents contained in the first solution S1. The temperature of the first solution S1 is, for example, about -20°C. In this embodiment, "temperature" refers to the temperature under atmospheric pressure.

[0038] The first container C1 is a rectangular plate-shaped box with an opening C1h at one end C1a. At least a portion of the wall portion constituting the first container C1 has a light-transmitting portion Cp that transmits excitation light L1 and detection light L2, which will be described later. In this case, the light-transmitting portion Cp is provided for one wall portion of the first container C1 that forms the opening C1h. Inside the first container C1 (near the bottom), a rotor R1 is provided at a position where it is immersed in the first solution S1. The first container C1, the first solution S1, and the rotor R1 constitute a single measurement kit. Therefore, the first container C1, the first solution S1, and the rotor R1 are integrally supported by the measurement unit 10 as a measurement kit.

[0039] The second container C2 contains the second solution S2. The second solution S2 is a mixture of several reagents. The second solution S2 contains, for example, an stimulant and an organic solvent as reagents. The stimulant is a reagent used to activate the function of a biological sample. For example, the stimulant artificially stimulates neutrophil cells in the biological sample. When neutrophil cells are artificially stimulated, an innate immune response (biological defense response) of the neutrophil cells is triggered. Examples of stimulants include fMLP (N-formyl-L-methionyl-L-leucyl-phenylalanine) or PMA (4β-phorbol-12-myristate-13-acetate). The organic solvent is a reagent used to dissolve the stimulant in powder form. Examples of organic solvents include DMSO (Dimethyl sulfoxide).

[0040] The second solution S2 is frozen in the second container C2. The second solution S2 may be completely frozen or partially frozen. The temperature of the second solution S2 is below the freezing point of any of the reagents contained in the second solution S2. That is, the temperature of the second solution S2 may be the same as the freezing point of any of the reagents contained in the second solution S2, or it may be lower than the freezing point of any of the reagents contained in the second solution S2. In this embodiment, the temperature of the second solution S2 is below the freezing point of the reagent with the largest volume among the multiple reagents contained in the second solution S2. The temperature of the second solution S2 may also be below the freezing point of the reagent with the lowest freezing point among the multiple reagents contained in the second solution S2. In this embodiment, the temperature of the second solution S2 is below the freezing point of the organic solvent contained in the second solution S2.

[0041] In this embodiment, the overall temperature of the second container C2 is below the freezing point of any of the reagents contained in the second solution S2. The temperature of the second solution S2 is lower than the temperature of the first solution S1. For example, the temperature of the second solution S2 is -40°C or lower. In this embodiment, the temperature of the second solution S2 is -80°C. Alternatively, the temperature of the second solution S2 may be -20°C or lower.

[0042] In this embodiment, the second solution S2 does not contain a buffer. Therefore, degradation of the stimulant due to the buffer is suppressed. Specifically, stimulants diluted with a buffer tend to degrade the longer the period until the second solution S2 is frozen. If the second solution S2 does not contain a buffer, degradation of the stimulant is suppressed even if the period until the second solution S2 is frozen is relatively long.

[0043] Furthermore, the second solution S2 may also contain a buffer. The buffer is a reagent used to dilute the organic solvent. Since the organic solvent in the second solution S2 is diluted by the buffer, even if the second solution S2 is applied directly to a biological sample, the risk of the biological sample being harmed by the organic solvent is suppressed. Commercially available products may be used as the stimulant, organic solvent, and buffer. However, when the organic solvent is diluted by the buffer, the stimulant dissolved in the organic solvent may degrade easily. In contrast, by lowering the temperature of the second solution S2 to that of the first solution S1 as described above (by making it cryogenically cold), it becomes possible to store the second solution S2 while maintaining a state in which the degradation of the stimulant dissolved in the organic solvent is suppressed, even if the organic solvent is diluted by the buffer for direct application of the second solution S2 to a biological sample.

[0044] The second container C2 is formed in a conical shape, for example, having openings at one end C2a and the other end C2b. The one end C2a of the second container C2 corresponds to, for example, the apex of the cone and is the outlet for the second solution S2. The other end C2b of the second container C2 corresponds to, for example, the base of the cone and is attached to the first nozzle 13, which will be described later, and is the air inlet from the first nozzle 13. The second container C2 is, for example, a pipette tip. The second container C2 and the second solution S2 are integrally supported by the measurement unit 10 as a measurement kit.

[0045] The third container C3 contains a biological sample S3. The biological sample S3 is collected from a subject and placed inside the third container C3. The third container C3 is formed in a conical shape with openings at one end C3a and the other end C3b. The one end C3a of the third container C3 corresponds to, for example, the apex of the cone and is the outlet (and inlet) for the biological sample S3. The other end C3b of the third container C3 corresponds to, for example, the base of the cone and is attached to the second nozzle 15, which will be described later, and is the inlet (and outlet) for air from the second nozzle 15. In the first modified example, the third container C3 may further contain a fluorescent indicator in addition to the biological sample S3. Alternatively, in the first modified example, multiple third containers C3 may be prepared, with the biological sample S3 contained in one third container C3 and the fluorescent indicator in another third container C3. Furthermore, in the first modified example, the fluorescent indicator may be cooled or frozen while contained in the third container C3, as described later.

[0046] As will be described later, pipetting can be performed in the third container C3. In this case, liquid is repeatedly introduced into and out of the third container C3. When liquid is introduced into the third container C3, the other end C3b becomes an air inlet from the second nozzle 15, and the one end C3a becomes the outlet for the liquid. When liquid is introduced into the third container C3, the other end C3b becomes an air outlet to the second nozzle 15, and the one end C3a becomes the inlet for the liquid. The third container C3 is, for example, a pipette tip. In the first modified example, at least one of the two ends C3a and C3b may be an inlet for a liquid, such as a fluorescent indicator, into the third container C3.

[0047] In the measuring device 1, the measuring unit 10 supports (holds) the first container C1, the second container C2, and the third container C3. More specifically, the measuring unit 10 includes a holder 11 for the first container C1, a first nozzle 13, and a second nozzle 15. The holder 11 is provided with an insertion part 12 (support part) for the first container C1. The insertion part 12 supports the first container C1 in a fixed position within the measuring unit 10 by holding the first container C1. The insertion part 12 supports the first container C1 in a position where the opening C1h of one end C1a of the first container C1 faces vertically upward (opens), and the light-transmitting part Cp of the first container C1 faces the optical part 27, which will be described later.

[0048] The first nozzle 13 is located vertically above the insertion section 12. The first nozzle 13 is positioned so as to face the opening C1h of the first container C1 when the insertion section 12 is supporting the first container C1 (hereinafter referred to as the "supported state"). The second container C2 is attached to the first nozzle 13. The second container C2 is attached to the first nozzle 13 by inserting (for example, fitting) the tip of the first nozzle 13 into the other end C2b of the second container C2. In other words, the first nozzle 13 supports (holds) the second container C2 within the measuring section 10 such that one end C2a of the second container C2 faces the opening C1h side of the first container C1.

[0049] The second nozzle 15 is located above the insertion section 12. In the supported state, the second nozzle 15 is positioned to face the opening C1h of the first container C1. The first nozzle 13 and the second nozzle 15 are arranged, for example, along the horizontal direction. The third container C3 is attached to the second nozzle 15. The third container C3 is attached to the second nozzle 15 by inserting (for example fitting) the tip of the second nozzle 15 into the other end C3b of the second nozzle 15. In other words, the second nozzle 15 supports (holds) the third container C3 within the measuring section 10 such that one end C3a of the third container C3 faces the opening C1h side of the first container C1.

[0050] The measuring unit 10 is provided with a measuring unit cover (not shown). This allows the measuring unit 10 to switch between a state open to the outside (cover open) and a state shielded from the outside (cover closed) by opening and closing the measuring unit cover.

[0051] The measuring device 1 further comprises a first adjustment unit 21, a second adjustment unit 22, blowers 23, 24, a heater 25, a motor 26, an optical unit 27, and a circuit board 28. The first adjustment unit 21 is for adjusting the position at which the second solution S2 is added to the liquid surface T of the first solution S1 in the first container C1 while it is in a supported state. The first adjustment unit 21 is, for example, an electric slider to which the first nozzle 13 is attached. The first adjustment unit 21 adjusts the position in the height direction of one end C2a of the second container C2 (i.e., the outlet for the second solution S2) attached to the first nozzle 13 by moving the first nozzle 13 along the height direction (here, the vertical up and down direction) that intersects the liquid surface T of the first solution S1, thereby adjusting the position at which the second solution S2 is added to the liquid surface T in that height direction.

[0052] The second adjustment unit 22 is for adjusting the position of adding the biological sample S3 to the first solution S1 in the first container C1 while in a supported state. In the first modified example, the second adjustment unit 22 may be for adjusting the position of adding the biological sample S3 and a fluorescent indicator to the first solution S1 in the first container C1 while in a supported state. The second adjustment unit 22 is, for example, an electric slider to which a second nozzle 15 is attached. The second adjustment unit 22 adjusts the position in the height direction of one end C3a of the third container C3 attached to the second nozzle 15 (i.e., the outlet for the biological sample S3), thereby adjusting the position of adding the biological sample S3 in the height direction by moving the second nozzle 15 along the height direction. In the first modified example, the second adjustment unit 22 can adjust the position in the height direction of one end C3a of the third container C3 attached to the second nozzle 15 (i.e., the outlet for the biological sample S3 and fluorescent indicator) by moving the second nozzle 15 along the height direction, thereby adjusting the placement position of the biological sample S3 and fluorescent indicator in the height direction.

[0053] In this embodiment, the measuring device 1 is equipped with a first adjustment unit 21 that moves the first nozzle 13 along the height direction and a second adjustment unit 22 that moves the second nozzle 15 along the height direction, and these units are capable of operating independently of each other. However, the measuring device 1 may also be equipped with a single adjustment unit that combines the first adjustment unit 21 and the second adjustment unit 22, and moves both the first nozzle 13 and the second nozzle 15 together along the height direction.

[0054] The blower 23 is connected to the first nozzle 13 and the second nozzle 15, and is used to discharge air to the first nozzle 13 and the second nozzle 15, and to draw air from the second nozzle 15. By discharging air to the first nozzle 13, the blower 23 introduces air into the second container C2 from the other end C2b of the second container C2 attached to the first nozzle 13, and causes the second solution S2 to be discharged from one end C2a of the second container C2. In other words, the first nozzle 13 and the blower 23, in their supported state, constitute a first adding unit 41 for adding the second solution S2 to the first solution S1 in the first container C1.

[0055] Furthermore, the blower 23 discharges air to the second nozzle 15, introducing air into the third container C3 from the other end C3b of the third container C3 attached to the second nozzle 15, and causing the biological sample S3 to be discharged from one end C3a of the third container C3. The blower 23 also sucks air from the second nozzle 15, discharging the air from inside the third container C3 from the other end C3b of the third container C3, and introducing the first solution S1 into the third container C3 from one end C3a of the third container C3. In this way, the second nozzle 15 and the blower 23 constitute a second adding unit 42 for adding the biological sample S3 to the first solution S1 in the first container C1 when in the supported state. Note that when pipetting is performed in the third container C3 as described later, the liquid initially discharged from the third container C3 is the biological sample S3, and the liquid introduced into the third container C3 is the liquid in which the biological sample S3 has been added to the first solution S1. Furthermore, the liquid dispensed from the third container C3 from the second time onward is a mixture of the biological sample S3 and the first solution S1. In the first modified example, the blower 23 discharges air to the second nozzle 15, introducing air into the third container C3 from the other end C3b attached to the second nozzle 15, and allowing the biological sample S3 and fluorescent indicator to be dispensed from one end C3a of the third container C3. In the first modified example, the second nozzle 15 and the blower 23 can, in a supported state, constitute a second adding section 42 for adding the biological sample S3 and fluorescent indicator to the first solution S1 in the first container C1. Furthermore, when pipetting is performed in the third container C3, the liquid initially dispensed from the third container C3 is the biological sample S3 and fluorescent indicator, and the liquid introduced into the third container C3 is the liquid in which the biological sample S3 and fluorescent indicator have been added to the first solution S1. Furthermore, the liquid dispensed from the third container C3 from the second time onward is a mixture of the biological sample S3, the fluorescent indicator, and the first solution S1. In the first modified example, if multiple third containers C3 are used, multiple second additive units 42 may be formed by each of the multiple second nozzles 15 to which each of the multiple third containers C3 is attached, and the blower 23.For example, if a biological sample S3 is contained in one third container C3 and a fluorescent indicator is contained in another third container C3, two second additive units 42 may be formed by a second nozzle 15 and blower 23 to which one third container C3 is attached, and a second nozzle 15 and blower 23 to which the other third container C3 is attached.

[0056] In this embodiment, one blower 23 is provided for the first nozzle 13 and the second nozzle 15, but separate blowers that operate independently of each other may be provided for the first nozzle 13 and the second nozzle 15, respectively.

[0057] Blower 24 is for supplying air to the first container C1 in the supported state. In the supported state, blower 24 supplies air A to at least the surface of the light-transmitting portion Cp of the first container C1. For example, blower 24 can form an airflow that flows vertically upward on the wall surface of the first container C1, including the surface of the light-transmitting portion Cp. This allows blower 24 to forcibly ventilate the space near the light-transmitting portion Cp. Note that blower 24 may be shared with blower 23.

[0058] The heater 25 is provided on the holder 11 of the measuring unit 10. In this embodiment, the heater 25 is positioned in the supported state so as to face the wall opposite to the wall on which the light-transmitting portion Cp is provided in the first container C1. The heater 25 is for heating the first solution S1 in the first container C1 in the supported state. The heater 25 can thaw the first solution S1 in the first container C1 by heating it. After the first solution S1 in the first container C1 has thawed, the heater 25 can heat the first solution S1 so that its temperature reaches a suitable temperature (optimal temperature) for the biological sample S3, or heat the first solution S1 to maintain that temperature.

[0059] The motor 26 is provided in the holder 11 of the measuring unit 10. In this embodiment, the motor 26 is positioned in the supported state to face the wall opposite to the wall of the first container C1 on which the light-transmitting portion Cp is provided. In the supported state, the motor 26 is positioned to face the rotor R1 inside the first container C1. The motor 26 constitutes, for example, a magnetic stirrer and stirs the first solution S1 by rotating the rotor R1.

[0060] Figure 4 is a schematic diagram showing the configuration of the optical unit shown in Figure 2. As shown in Figures 2 and 4, the optical unit 27 is positioned to face the light-transmitting section Cp of the first container C1 when in the supported state. The optical unit 27 is for irradiating a mixture of the thawed first solution S1, the second solution S2 added to the first solution S1, and the biological sample S3 with excitation light L1, and for detecting the detection light L2 containing fluorescence generated in the mixture as a result of the irradiation with excitation light L1 when in the supported state. In the first modified example, the optical unit 27 may be for irradiating a mixture of the thawed first solution S1, the second solution S2 added to the first solution S1, the biological sample S3, and a fluorescent indicator with excitation light L1, and for detecting the detection light L2 containing fluorescence generated in the mixture as a result of the irradiation with excitation light L1 when in the supported state.

[0061] The optical section 27 includes a light source 61, an optical filter 62, and a lens barrel section 63. The light source 61 includes, for example, a light-emitting element such as a laser diode or a light-emitting diode. In the supported state, the light source 61 emits excitation light L1 toward the light-transmitting section Cp. The optical filter 62 is provided on the optical path of the excitation light L1 emitted from the light source 61. The optical filter 62 transmits some of the wavelength components of the excitation light L1 emitted from the light source 61. The optical filter 62 is, for example, a short-pass filter that transmits wavelength components of 490 nm or less.

[0062] In this manner, the optical unit 27 irradiates the mixture in the first container C1 with excitation light L1 via the light-transmitting unit Cp. As a result, substances produced by the reaction between components generated from the biological sample S3 and the fluorescent indicator in the first solution S1 are excited within the first container C1, and fluorescence is generated. Consequently, detection light L2 containing this fluorescence is emitted from the light-transmitting unit Cp of the first container C1.

[0063] The lens barrel 63 includes a lens 64, optical filters 65-67, lens 68, and a detector 69, which are arranged in sequence along the optical path of the detection light L2 emitted through the light transmission section Cp. Lenses 64 and 68 are, for example, plano-convex lenses that are convex in opposite directions to each other, and they focus the detection light L2 emitted from the light transmission section Cp toward the detector 69. Lenses 64 and 68 constitute a relay lens system.

[0064] The optical filter 65 selectively transmits detection light L2 emitted from the lens 64 that is incident at an incident angle of a predetermined angle (e.g., 10°) or less. The optical filter 65 is, for example, a honeycomb filter. Optical filters 66 and 67 transmit some wavelength components of the detection light L2 from the optical filter 65. Optical filter 66 is, for example, a long-pass filter that transmits wavelength components of 530 nm or more. Optical filter 67 is, for example, a long-pass filter that transmits wavelength components of 515 nm or more. Lens 68 focuses the detection light L2 that has passed through optical filters 65 to 67. Detector 69 detects the detection light L2 focused by lens 68. Detector 69 includes, for example, a photodiode.

[0065] The circuit board 28 is electrically connected to each part of the measuring device 1. The circuit board 28 may also contain a control unit that controls each part of the measuring device 1. In this case, the control unit can control, for example, at least one of the following: movement of the first nozzle 13 by the first adjustment unit 21, movement of the second nozzle 15 by the second adjustment unit 22, discharge and suction of air by the blower 23, supply of air to the first container C1 by the blower 24, and emission of excitation light L1 by the light source 61.

[0066] Here, the adjustment of the addition position of the second solution S2 by the first adjustment unit 21 and the adjustment of the addition position of the biological sample S3 by the second adjustment unit 22 will be explained in more detail. Figure 5 is a schematic front view showing how the addition position of the second solution is adjusted. As shown in Figures 3 and 5, the first adjustment unit 21 moves the first nozzle 13 along the height direction so that one end C2a of the second container C2 attached to the first nozzle 13 (i.e., the outlet and addition position of the second solution S2) is at an appropriate distance from the liquid surface T of the first solution S1 contained in the first container C1.

[0067] According to the inventors' findings, if the distance D2 between one end C2a of the second container C2 and the liquid surface T of the first solution S1 is large, such as 3 mm or more, it may not be possible to completely discharge the second solution S2 from the second container C2 due to liquid backflow. In contrast, if the distance D2 between one end C2a of the second container C2 and the liquid surface T of the first solution S1 is 1 mm or less, it becomes possible to sufficiently discharge the second solution S2 from the second container C2. On the other hand, if one end C2a of the second container C2 is located inside the first solution S1, although it is possible to sufficiently discharge the second solution S2 from the second container C2, the turbulence of the liquid surface T when the second solution S2 is added becomes large.

[0068] Therefore, when the second solution S2 is added to the first solution S1, the first adjustment unit 21 moves the first nozzle 13 along the height direction so that the distance D2 from the liquid surface T in the height direction of the outlet (one end C2a) of the second solution S2 in the second container C2 is 1 mm or less in the range where the one end C2a does not come into contact with the liquid surface T. This process may also be carried out by a control unit configured on the circuit board 28 controlling the first adjustment unit 21.

[0069] Furthermore, if one end C2a of the second container C2 is positioned horizontally at a position P2 approximately 1 / 4 of the way from the edge of the liquid surface T, and the distance D1 from one end C1a of the first container C1 to the liquid surface T at that position P2 is approximately 5 mm, then the first adjustment unit 21 can move the first nozzle 13 in the height direction so that one end C2a of the second container C2 is approximately 4 mm (= distance D1 - distance D2). In addition, the distance D2 may be 0.5 mm or less (for example, approximately 0.5 mm). In this case, the first adjustment unit 21 can move the first nozzle 13 in the height direction so that one end C2a of the second container C2 is approximately 4.5 mm (= distance D1 - distance D2) from one end C1a of the first container C1.

[0070] On the other hand, when adding the biological sample S3 to the first solution S1, for example, in response to the requirement to perform pipetting, the second adjustment unit 22 moves the second nozzle 15 along the height direction so that one end C3a of the third container C3 (i.e., the outlet for the biological sample S3) is located inside the first solution S1. This allows the second addition unit 42 to perform suitable pipetting by repeatedly performing the process of discharging the biological sample S3 from the third container C3 using the second nozzle 15 and introducing the first solution S1 into the third container C3 using the second nozzle 15. Such processing may also be performed by a control unit configured on the circuit board 28 controlling the second adjustment unit 22 and the blower 23. In the first modified example, the second adjustment unit 22 can move the second nozzle 15 along the height direction so that one end C3a of the third container C3 (i.e., the outlet for the biological sample S3 and the fluorescent indicator) is located inside the first solution S1. As a result, the second additive unit 42 can suitably perform pipetting by repeatedly performing the following actions: drawing out the biological sample S3 and fluorescent indicator from the third container C3 using the second nozzle 15, and introducing the first solution S1 into the third container C3 using the second nozzle 15.

[0071] Next, an example of the preparation steps for a measurement method using the measuring device 1 according to the above embodiment will be described. Figure 6 is a flowchart showing each step corresponding to the preparation stage of the measurement method according to this embodiment. As shown in Figure 6, first, a first container C1 containing the rotor R1 is prepared (step S11). At the same time, a first solution S1 is prepared by mixing several reagents (at least one of physiological saline and buffer solution, and a fluorescent indicator, etc.) outside the first container C1 (step S12). In the first modified example, a first solution S1 without a fluorescent indicator can be prepared in step S12. Subsequently, the pre-prepared first solution S1 is introduced into the first container C1 (step S13). In the first modified example, a fluorescent indicator can be introduced into the third container C3 in step S13.

[0072] Next, the first solution S1 is frozen in the first container C1 (step S14). In the first modified example, in step S14, the fluorescent indicator can be cooled to, for example, -20°C in the third container C3. In step S14, the first solution S1 is frozen by placing the first container C1 in the cooling space of a cooling facility with the rotor R1 contained in the first container C1 immersed in the first solution S1. The temperature of the cooling space is, for example, about -20°C. The first container C1 is placed in the cooling space for, for example, about 2 hours. As a result, the first solution S1 is frozen, and a measurement kit consisting of the first container C1, the first solution S1, and the rotor R1 is manufactured. The opening C1h of the first container C1 may be sealed with a sealing member such as Parafilm or a rubber stopper. In the first modified example, in step S14, the fluorescent indicator in the third container C3 may be cooled to, for example, about -20°C, or the fluorescent indicator in the third container C3 may be frozen to produce a measurement kit including the third container C3.

[0073] Meanwhile, a second container C2 is prepared (step S15). At the same time, a second solution S2 is prepared by mixing several reagents (stimulant, organic solvent, etc.) outside the second container C2 (step S16). Subsequently, the pre-prepared second solution S2 is introduced into the second container C2 (step S17). After that, the second container C2 is placed in the cooling space of the cooling equipment, thereby freezing the second solution S2 while it is contained in the second container C2 (step S18). The temperature of the cooling space is, for example, about -80°C. The second container C2 is placed in the cooling space for, for example, about 1 hour. As a result, the second solution S2 is frozen, and a measurement kit consisting of the second container C2 and the second solution S2 is manufactured. As described above, a measurement unit is manufactured by two measurement kits: a measurement kit containing the first container C1 manufactured in step S14, and a measurement kit containing the second container C2 manufactured in step S18. On the other hand, in the first modified example, a measurement unit can be manufactured using three measurement kits: a measurement kit including a first container C1 manufactured in step S14, a measurement kit including a third container C3, and a measurement kit including a second container C2 manufactured in step S18.

[0074] Next, an example of a measurement method using the measuring device 1 according to the above embodiment will be described. Figure 7 is a flowchart of each step of the measurement method according to this embodiment. As shown in Figure 7, first, as preparation for the measuring device 1, after turning on the power to the measuring device 1, the measuring device 1 is warmed up (not shown) until the temperature of the insertion part 12 of the measuring unit 10 reaches an appropriate temperature (for example, 37°C). As an example, the temperature of the insertion part 12 may be displayed on the display unit 30.

[0075] Subsequently, after the warm-up operation of the measuring device 1 is completed, the first container C1, the second container C2, and the third container C3 are prepared and set in the measuring device 1 (step S21). In step S21, the first container C1 contains the frozen first solution S1 and the rotor R1. The second container C2 contains the frozen second solution S2. Furthermore, the third container C3 contains the biological sample S3 taken from the subject. In the first modified example, the third container C3 may contain a fluorescent indicator and the biological sample S3. Then, in step S21, the first container C1 is set in the measuring device 1 by holding it in the insertion part 12 of the measuring unit 10, the second container C2 is set in the measuring device 1 by attaching it to the first nozzle 13, and the third container C3 is set in the measuring device 1 by attaching it to the second nozzle 15.

[0076] Specifically, in step S21, a first container C1 containing a frozen first solution S1 that includes an indicator that reacts with components generated from the biological sample S3 is supported in the insertion section 12 of the measuring device 1 (first step). Also in step S21, a second container C2 containing a second solution S2 that includes a stimulant that activates the function of the biological sample S3 is attached to the first nozzle 13 of the measuring device 1 (third step). Furthermore, in step S21, a third container C3 containing the biological sample S3 is attached to the second nozzle 15 of the measuring device 1 (eighth step). After that, the user may input the sample name via the display unit 30. In the first modified example, in step S21, a first container C1 containing a frozen first solution S1 for diluting the biological sample S3 is supported in the insertion section 12 of the measuring device 1 (first step). Furthermore, in the first modified example, in step S21, the third container C3, which contains the biological sample S3 and the fluorescent indicator, is attached to the second nozzle 15 of the measuring device 1 (step 8). In the first modified example, if the fluorescent indicator in the third container C3 is cooled to, for example, about -20°C, the fluorescent indicator in the third container C3 may be raised by, for example, keeping the third container C3 in a room temperature environment for a certain period of time before introducing the biological sample S3 into the third container C3. Alternatively, in the first modified example, if the fluorescent indicator in the third container C3 is frozen, the fluorescent indicator in the third container C3 may be naturally thawed by, for example, keeping the third container C3 in a room temperature environment for a certain period of time before introducing the biological sample S3 into the third container C3.

[0077] Next, while in the supported state, the first solution S1 in the first container C1 is heated by the heater 25 to thaw the first solution S1 in the first container C1 (step S22, second step). In step S22, for example, the heating of the first solution S1 by the heater 25 is maintained so that the temperature of the first solution S1 reaches a suitable temperature (optimal temperature) for the biological sample S3 (for example, heating is maintained for about 15 minutes). The suitable temperature for the biological sample S3 is, for example, 37°C.

[0078] In step S22, the first solution S1 is heated while the rotor R1 is rotated by the motor 26. Specifically, in step S22, the rotor R1 is rotated after the first solution S1 has thawed and become fluid. When the rotor R1 rotates, the first solution S1 is stirred in the first container C1, which promotes heat transfer in the first solution S1 and improves the heating efficiency of the first solution S1. In step S22, the motor 26 may be started before the first solution S1 becomes fluid. In this case, the rotor R1 rotates at the same time that the first solution S1 becomes fluid. Also, in step S22, when the temperature of the first solution S1 reaches an appropriate temperature, information to inform the user that the heating of the first solution S1 is complete may be presented to the user (for example, by sounding a buzzer or by displaying on the display unit 30).

[0079] In the measurement method according to this embodiment, when the first solution S1 is thawed in step S22, air is supplied to the surface of the light-transmitting portion Cp of the first container C1 using the blower 24 of the measuring device 1. This forces ventilation between the light-transmitting portion Cp and the optical portion 27, thereby suppressing condensation on the surface of the light-transmitting portion Cp and removing any condensation that has formed on the surface of the light-transmitting portion Cp.

[0080] In the subsequent step, the addition position of the biological sample S3 to the first solution S1 in the first container C1 is adjusted (step S23, step 9). More specifically, in step S23, the second adjustment unit 22 of the measuring device 1 moves the second nozzle 15 to which the third container C3 is attached along the height direction, thereby adjusting the position of one end C3a of the third container C3, which is the addition position of the biological sample S3 to the first solution S1, in the height direction. In particular, in step S23, the second adjustment unit 22 moves the second nozzle 15 along the height direction so that one end C3a of the third container C3 is located inside the first solution S1, as shown in Figure 3. In the first modified example, in step S23, the addition positions of the biological sample S3 and the fluorescent indicator to the first solution S1 in the first container C1 can be adjusted. More specifically, in the first modified example, in step S23, the second adjustment unit 22 of the measuring device 1 can adjust the position of one end C3a of the third container C3, which is the addition position of the biological sample S3 and the fluorescent indicator to the first solution S1, in the height direction by moving the second nozzle 15 to which the third container C3 is attached along the height direction.

[0081] Next, air is introduced from the second nozzle 15 to the third container C3 by discharging air from the blower 23, and the biological sample S3 is discharged from one end C3a of the third container C3 to add the biological sample S3 to the first solution S1 in the first container C1 (step S24, step 6). As described above, the third container C3 containing the biological sample S3 is configured such that one end C3a, which is the outlet for the biological sample S3, is located in the first solution S1. Therefore, in step S24, the biological sample S3 is added in the first solution S1. In the first modified example, in step S24, air is introduced from the second nozzle 15 to the third container C3 by discharging air from the blower 23, and the biological sample S3 and fluorescent indicator are discharged from one end C3a of the third container C3 to add the biological sample S3 and fluorescent indicator to the first solution S1 in the first container C1. In the first modified example, the third container C3 containing the biological sample S3 and the fluorescent indicator may be configured such that one end C3a, which is the outlet for the biological sample S3 and the fluorescent indicator, is located in the first solution S1. Therefore, in the first modified example, in step S24, the biological sample S3 and the fluorescent indicator may be added in the first solution S1.

[0082] Furthermore, in step S24, when adding the biological sample S3 to the first solution S1, the discharge of air from the blower 23 to the second nozzle 15 and the suction of air from the second nozzle 15 are repeated alternately, thereby repeatedly performing the extraction of the biological sample S3 from the third container C3 by the second nozzle 15 and the introduction of the first solution S1 into the third container C3 by the second nozzle 15. In other words, pipetting is performed in step S24. At this time, as an example, a sequence of 400 ms discharge, 200 ms suction, 200 ms discharge, 200 ms suction, and 400 ms discharge can be performed. In the first modified example, in step S24, when adding the biological sample S3 and fluorescent indicator to the first solution S1, the discharge of air from the blower 23 to the second nozzle 15 and the suction of air from the second nozzle 15 are repeatedly performed alternately, thereby repeatedly releasing the biological sample S3 and fluorescent indicator from the third container C3 by the second nozzle 15 and introducing the first solution S1 into the third container C3 by the second nozzle 15.

[0083] After the addition of the biological sample S3 (or the biological sample S3 and fluorescent indicator in the first modified example) is completed in step S24, the second adjustment unit 22 can raise the second nozzle 15 to the standby position.

[0084] In the next step, the heater 25 is used to adjust the temperature of the first solution S1 (step S25). In step S25, the first solution S1 is heated by the heater 25 to maintain the temperature of the first solution S1 at the appropriate temperature for the biological sample. For example, in step S25, the first solution S1 can be heated so that its temperature is, for example, 36.8°C to 37.2°C. Furthermore, in step S25, the first solution S1 can be heated while the rotor R1 is rotated by the motor 26. Step S25 may be continued until the measurement of the characteristics of the biological sample S3 is completed.

[0085] Next, the second solution S2 is thawed (step S26). More specifically, in step S26, with the second container C2 attached to the first nozzle 13, the second solution S2 is allowed to thaw naturally by leaving the second container C2 for a predetermined time (for example, 1 to 2 minutes). Therefore, step S26 can be performed simultaneously with other steps after the second container C2 has been attached to the first nozzle 13.

[0086] Next, the addition position of the second solution S2 relative to the liquid surface T of the first solution S1 (here, a mixture of the first solution S1 and the biological sample S3) in the first container C1 is adjusted (step S27, fourth step). More specifically, in step S27, the first adjustment unit 21 moves the first nozzle 13 along the height direction to adjust the position of one end C2a of the second container C2, which is the addition position of the first solution S1 relative to the liquid surface T, in the height direction. In particular, in step S27, as shown in Figure 5, the first adjustment unit 21 moves the first nozzle 13 along the height direction so that the distance D2 from the liquid surface T of one end C2a (the outlet for the second solution S2) of the second container C2 is 1 mm or less in the range where one end C2a does not come into contact with the liquid surface T.

[0087] Next, the second solution S2 is added to the first solution S1 in the first container C1 (step S28, fifth step). More specifically, in step S28, with the addition position of the second solution S2 adjusted as described above, the blower 23 discharges air from the first nozzle 13 to introduce air from the first nozzle 13 into the second container C2, and the second solution S2 is drawn out from one end C2a of the second container C2, thereby adding the second solution S2 to the first solution S1 in the first container C1.

[0088] After the addition of the second solution S2 is completed in step S28, the first adjustment unit 21 can raise the first nozzle 13 to the standby position.

[0089] Next, the optical unit 27 of the measuring device 1 irradiates the mixture of the first solution S1, the second solution S2 added to the first solution S1, and the biological sample S3 with excitation light L1, and detects the detection light L2 containing fluorescence generated in the mixture as a result of the irradiation with excitation light L1, thereby measuring the properties of the biological sample S3 (step S29, seventh step). In the first modified example, in step S29, the optical unit 27 of the measuring device 1 irradiates the mixture of the first solution S1, the second solution S2 added to the first solution S1, the biological sample S3, and a fluorescent indicator with excitation light L1, and detects the detection light L2 containing fluorescence generated in the mixture as a result of the irradiation with excitation light L1, thereby measuring the properties of the biological sample S3. As described above, the excitation light L1 is emitted from the light source 61 of the optical unit 27, and the detection light L2 is detected by the detector 69 of the optical unit 27.

[0090] In step S29, the mixture in the first container C1 is continuously irradiated with excitation light L1, and detection light L2 from the first container C1 is continuously detected. Irradiation with excitation light L1 and detection of detection light L2 can be started before the addition of the second solution S2 in step S28. When excitation light L1 with a wavelength of approximately 480 nm is irradiated onto the first container C1, where the reaction between the fluorescent indicator and HOCl generated from the biological sample S3 is proceeding, fluorescence with a wavelength of approximately 515 nm is generated in the first container C1. In step S29, detection light L2 containing fluorescence with a wavelength of approximately 515 nm is detected. This allows for the measurement of myeloperoxidase activity.

[0091] Furthermore, after step S29, evaluation values ​​may be calculated based on the measurement results, or information indicating the measurement results and evaluation values ​​may be displayed on the display unit 30 or printed by the output unit 50. Also, after steps S24 (sixth step) and S28 (fifth step), and before step S29 (seventh step), a step may be performed in which the mixture in the first container C1 is stirred, for example, by a rotor R1.

[0092] As described above, in the measuring device 1 and measuring method according to this embodiment, the first container C1 containing the frozen first solution S1 is supported by a support part, and in this state, the first solution S1 can be thawed by the heater 25. This allows the first solution S1 to be thawed near the subject (in the measuring device 1), and then the biological sample S3 collected from the subject can be added to the first solution S1. Therefore, not only is the quality of the first solution S1 maintained, but tasks such as preparing the first solution S1 and introducing the first solution S1 into the first container C1 are not required near the subject.

[0093] Therefore, according to the measuring device 1 and measuring method of this embodiment, the quality of the first solution S1 is maintained and the work for measuring the characteristics of the biological sample S3 is simplified. Furthermore, in the measuring device 1 and measuring method of this embodiment, the first adjustment unit 21 adjusts the addition position of the second solution S2 relative to the liquid surface T of the first solution S1 by moving the first nozzle 13 to which the second container C2 is attached along the height direction intersecting the liquid surface T of the first solution S1. This makes it possible to add the second solution S2 to the first solution S1 at an appropriate addition position relative to the liquid surface T of the first solution S1.

[0094] Furthermore, in the measuring device 1 according to this embodiment, when the second solution S2 is added to the first solution S1, the first adjustment unit 21 moves the first nozzle 13 along the height direction so that the distance D2 from the liquid surface T in the height direction of the outlet of the second solution S2 in the second container C2 is 1 mm or less in the range in which the outlet does not come into contact with the liquid surface T.

[0095] Furthermore, in the measurement method according to this embodiment, in step S27, when the second solution S2 is added to the first solution S1, the first adjustment unit 21 of the measuring device 1 moves the first nozzle 13 along the height direction so that the distance from the liquid surface T in the height direction of the outlet of the second solution S2 in the second container C2 is 1 mm or less in the range in which the outlet does not come into contact with the liquid surface T.

[0096] By discharging the second solution S2 from the second container C2 at a distance D2 of 1 mm or less from the liquid surface T of the first solution S1, it is possible to suppress the residue of the second solution S2 remaining in the second container C2. Furthermore, it is possible to suppress the amount of second solution S2 that splashes onto the inner wall surface of the first container C1 after being discharged from the second container C2. In addition, by ensuring that the outlet for the second solution S2 in the second container C2 does not come into contact with the first solution S1, it is possible to suppress significant disturbance of the liquid surface of the first solution S1 due to the addition of the second solution S2, thereby suppressing disturbance of the detection signal.

[0097] In particular, if the irradiation of the mixture in the first container C1 with excitation light L1 in step S29 and the detection of detection light L2 from the first container C1 begin before the addition of the second solution S2 in step S28, then in step S27, the first adjustment unit 21 of the measuring device 1 moves the first nozzle 13 along the height direction so that the distance from the liquid surface T in the height direction of the outlet of the second solution S2 in the second container C2 is 1 mm or less in the range where the outlet does not come into contact with the liquid surface T. This makes it more effective to suppress splashing of the second solution S2 onto the inner wall surface of the first container C1 and to suppress disturbance of the liquid surface of the first solution S1.

[0098] Furthermore, the measuring device 1 according to this embodiment includes a blower 24 for supplying air to the first container C1 when it is in a supported state. The first container C1 includes a light-transmitting section Cp that transmits excitation light L1 and detection light L2, and the blower 24 supplies air to the surface of the light-transmitting section Cp when it is in a supported state.

[0099] Furthermore, in the measurement method according to this embodiment, when thawing the first solution S1 in step S22, air is supplied to the surface of the light-transmitting portion Cp in the first container C1 that transmits the excitation light L1 and detection light L2 using the blower 24 of the measuring device 1.

[0100] In this way, by supplying air to the surface of the light-transmitting portion Cp in the first container C1 using the blower 24, it is possible to suppress condensation on the first container C1 when the first solution S1 is thawed, and to quickly remove any condensation that has formed on the first container C1. Therefore, the effect of condensation on the surface of the light-transmitting portion Cp in the first container C1 can be quickly reduced, and the measurement time can be shortened. Note that the measurement time refers to the total time from step S21 to step S29, not just the time spent monitoring the detection light L2 in step S29.

[0101] Furthermore, in the measuring device 1 according to this embodiment, the optical unit 27 includes an optical filter 65 for selectively transmitting detection light L2 that is incident at an incident angle of a predetermined angle or less, a lens 68 for focusing the detection light L2 that has passed through the optical filter 65, and a detector 69 for detecting the detection light L2 focused by the lens 68. Therefore, components affected by scattering contained in the detection light L2 can be removed by the optical filter 65. In addition, even if the amount of light in the detection light L2 decreases due to the removal of some components by the optical filter 65, the effect of this decrease in light amount on the detection result can be suppressed by focusing the detection light L2 with the lens 68 downstream of the optical filter 65.

[0102] Furthermore, it is preferable that the optical filter 65 is placed before optical filters 66 and 67, which transmit some of the wavelength components of the detected light L2. In this case, only the straight-traveling light that has passed through the optical filter 65 is incident on the optical filters 66 and 67, making it possible to reliably reduce scattered light.

[0103] Furthermore, the measuring device 1 according to this embodiment includes a second adjustment unit 22 for adjusting the addition position of the biological sample S3 (in the first modified example, the biological sample S3 and a fluorescent indicator (hereinafter the same)) to the first solution S1 in the first container C1 while in a supported state. The second addition unit 42 has a second nozzle 15 to which a third container C3 containing the biological sample S3 is attached, for distributing the biological sample S3 from the third container C3 to the first container C1. The second adjustment unit 22 adjusts the addition position of the biological sample S3 in the height direction by moving the second nozzle 15 along the height direction.

[0104] Furthermore, the measurement method according to this embodiment includes, before the sixth step, step S21 of attaching a third container containing the biological sample to the second nozzle of the measuring device, and, after step S21 and before step S24, step S23 of adjusting the addition position of the biological sample S3 to the first solution S1 in the first container C1. In step S23, the second adjustment unit 22 of the measuring device 1 adjusts the addition position of the biological sample S3 to the first solution S1 in the height direction by moving the second nozzle 15 along the height direction.

[0105] Therefore, it becomes possible to add the biological sample S3 to the first solution S1 at an appropriate addition position relative to the first solution S1.

[0106] Furthermore, in the measuring device 1 according to this embodiment, the second adjustment unit 22 moves the second nozzle 15 along the height direction so that the outlet for the biological sample S3 in the third container C3 is located inside the first solution S1.

[0107] Furthermore, in the measurement method according to this embodiment, in step S23, the second adjustment unit 22 of the measuring device 1 moves the second nozzle 15 along the height direction so that the outlet of the biological sample S3 in the third container C3 is located in the first solution S1.

[0108] In this case, it becomes possible to discharge the biological sample S3 from the third container C3 into the first solution S1.

[0109] Furthermore, in the measuring device 1 according to this embodiment, when adding the biological sample S3 to the first solution S1, the second addition unit 42 repeatedly performs the following actions: discharging the biological sample S3 from the third container C3 using the second nozzle 15, and introducing the first solution S1 into the third container C3 using the second nozzle 15.

[0110] Furthermore, in the measurement method according to this embodiment, when the biological sample S3 is added to the first solution S1 in step S24, the biological sample S3 is repeatedly drawn out of the third container C3 by the second nozzle 15, and the first solution S1 is repeatedly introduced into the third container C3 by the second nozzle 15.

[0111] In this way, by performing pipetting in the third container C3 using the second nozzle 15, it becomes possible to reliably add the biological sample S3 in the third container C3 to the first solution S1. In particular, by increasing the amount of liquid dispensed from the third container C3 during the final discharge in pipetting, it is possible to reliably suppress any remaining biological sample S3 in the third container C3.

[0112] Furthermore, in this embodiment, the second solution S2 does not contain a buffer. As a result, the total volume of the second solution S2 in the second container C2 is reduced, and the residual liquid in the second container C2 has a greater impact on the measurement accuracy. Therefore, as described above, the effect of suppressing residual liquid by discharging the second solution S2 from the second container C2 at a distance D2 of 1 mm or less from the liquid surface T of the first solution S1 becomes greater.

[0113] The above embodiments describe one aspect of the present invention. Therefore, the present invention is not limited to the above embodiments and can be modified as needed.

[0114] For example, the subject is not limited to the human body, but may be an animal, for example. Also, in the above embodiment, an example was shown where the biological sample S3 is the subject's blood, but the biological sample S3 may be, for example, other bodily fluids of the subject. The biological sample S3 may be, for example, the subject's saliva, perfusion fluid, tears, sweat, or urine. An example of the activity of neutrophil cells was shown as a characteristic of the biological sample S3, but the characteristic of the biological sample S3 may be the activity of tissue cells such as monocytes, eosinophils, basophils, B cells, T cells, NK cells, or vascular endothelial cells, for example.

[0115] Furthermore, although the above embodiment shows an example in which the first solution S1 contains a fluorescent indicator, the first solution S1 may also contain a chemiluminescent indicator. The chemiluminescent indicator reacts with components generated from the biological sample (e.g., superoxide). An example of a chemiluminescent indicator is MCLA (2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one). A commercially available chemiluminescent indicator may be used. In the first container C1, where the reaction between superoxide generated from the biological sample S3 and the chemiluminescent indicator is progressing, chemiluminescence with a maximum emission wavelength of approximately 465 nm is generated. Therefore, in step S29, chemiluminescence with a maximum emission wavelength of approximately 465 nm may be detected. This allows for the measurement of superoxide production activity. In the first modified example, the first solution S1 may not contain a chemiluminescent indicator, and the chemiluminescent indicator may be contained in the third container C3.

[0116] Furthermore, although the above embodiment shows an example where the second container C2 and the third container C3 are pipette tips, at least one of the second container C2 and the third container C3 may be, for example, a capillary nozzle, dropper, volumetric pipette, graduated pipette, measuring pipette, hematocrit capillary tube, or syringe.

[0117] Furthermore, the first solution S1 may also contain indicators that react with hypochlorous acid (or its halogen equivalent) produced by myeloperoxidase, such as taurine / TNB (see J. Clin. Invest., Vol. 70, pp. 598-607, 1982) and 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4-(2H,3H)dione (L-012: see Anal Biochem., Vol. 271(1), pp. 53-58, 1999). These indicators may also be contained in the third container C3 instead of being included in the first solution S1, similar to the fluorescent and chemiluminescent indicators described above.

[0118] Furthermore, the first solution S1 may also contain indicators that react with superoxide, such as 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazine-3-one (CLA), 2-methyl-6-p-methoxyphenylethinylimidazopyrazinon (MPEC), indocyanine-type imidazopyranodine compounds (NIR-CLA), and 2-[2,4,5,7-tetrafluoro-6-(2-nitro-4,5-dimethoxyphenylsulfonyloxy)-3-oxo-3H-xanthene-9-yl]benzoic acid (BES-So). These indicators may also be contained in the third container C3 without being included in the first solution S1, similar to the fluorescent and chemiluminescent indicators described above.

[0119] Furthermore, the second solution S2 may contain, for example, opsonized zymon (OZ) as an irritant.

[0120] Furthermore, although the above embodiment shows an example in which the first solution S1, which is prepared in advance outside the first container C1, is placed in the first container C1, the first solution S1 may also be prepared inside the first container C1. For example, each of the multiple reagents may be introduced into the first container C1 while separated from each other. Moreover, each of the multiple reagents may be introduced into the first container C1 sequentially or simultaneously.

[0121] Here, if the total amount of buffer required for the preparation of the first solution S1 and the amount of buffer required for the preparation of the second solution S2 is defined as a specific specified amount, the specified amount of buffer may be prepared in step S12 for the preparation of the first solution S1. In this case, in step S12, the first solution S1 containing the buffer required for the preparation of the first solution S1 and the buffer required for the preparation of the second solution S2 (i.e., containing the specified amount of buffer) may be prepared. Then, in step S13, the first solution S1 containing the specified amount of buffer may be introduced into the first container C1. In other words, the amount of buffer required for the preparation of the second solution S2 may also be introduced into the first container C1 in advance in step S13. Furthermore, the amount of buffer required for the preparation of the second solution S2 may be added to the first container C1 in step S28 before the addition of the second solution S2 to the first container C1. In these cases, the second solution S2 in the second container C2 will not contain buffer. In step S12, only the amount of buffer necessary for preparing the first solution S1 may be prepared, and the first solution S1 containing only that amount of buffer may be prepared. Furthermore, the amount of buffer contained in the first solution S1 may be adjusted as appropriate depending on whether the second solution S2 contains buffer or not.

[0122] Furthermore, when adding the second solution S2 to the first solution S1, it is not essential to move the first nozzle 13 along the height direction so that the distance D2 from the liquid surface T in the height direction of the outlet of the second solution S2 in the second container C2 is 1 mm or less in the range where the outlet does not come into contact with the liquid surface T. In other words, the first adjustment unit 21 may adjust the distance D2 in a range other than that range.

[0123] Furthermore, the measuring device 1 does not necessarily have a blower 24, and therefore does not need to supply air to the light-transmitting section Cp of the first container C1 by the blower 24. Also, the optical section 27 does not necessarily have an optical filter 65 for selectively transmitting detection light L2 that is incident at an incident angle of less than or equal to a predetermined angle.

[0124] Furthermore, the measuring device 1 does not necessarily have to be equipped with a second adjustment unit 22, and even if the measuring device 1 is equipped with a second adjustment unit 22, it is not essential that the second adjustment unit 22 move the second nozzle 15 along the height direction so that the outlet for the biological sample S3 in the third container C3 is located in the first solution S1. Moreover, the second adjustment unit 22 does not need to perform pipetting in the third container C3. [Explanation of Symbols]

[0125] 1... Measuring device, 12... Insertion part (support part), 13... First nozzle (first addition part 41), 15... Second nozzle (second addition part 42), 21... First adjustment part, 22... Second adjustment part, 23... Blower (first addition part, second addition part), 24... Blower, 25... Heater, 27... Optical part, 65... Optical filter, 68... Lens, 69... Detector, L1... Excitation light, L2... Detection light, C1... First container, C2... Second container, C3... Third container, S1... First solution, S2... Second solution, S3... Biological sample, T... Liquid level.

Claims

1. A measuring device used for measuring the properties of biological samples, A support for supporting a first container which contains a first solution that is frozen and contains an indicator that reacts with components generated from a biological sample, In the support state in which the support portion supports the first container, a heater for heating the first solution in the first container, In the aforementioned support state, the first addition section is for adding a second solution containing a stimulant that activates the function of the biological sample to the first solution in the first container, In the aforementioned support state, a second addition section for adding the biological sample to the first solution in the first container, In the aforementioned support state, a first adjustment unit for adjusting the position at which the second solution is added relative to the liquid surface of the first solution in the first container, In the aforementioned support state, the optical unit is for irradiating the mixture of the thawed first solution, the second solution added to the first solution, and the biological sample with excitation light, and for detecting detection light including fluorescence generated in the mixture as a result of the irradiation with the excitation light. Equipped with, The first additive unit is fitted with a second container containing the second solution and has a first nozzle for dispensing the second solution from the second container into the first solution. The first adjustment unit adjusts the position of the second solution relative to the liquid surface in the height direction by moving the first nozzle along the height direction intersecting the liquid surface. Measuring device.

2. A measuring device used for measuring the properties of biological samples, A support portion for supporting a first container that contains a frozen first solution for diluting the biological sample, In the support state in which the support portion supports the first container, a heater for heating the first solution in the first container, In the aforementioned support state, the first addition section is for adding a second solution containing a stimulant that activates the function of the biological sample to the first solution in the first container, In the aforementioned support state, a second additive unit is provided for adding an indicator that reacts with the biological sample and components generated from the biological sample to the first solution in the first container, In the aforementioned support state, a first adjustment unit for adjusting the position at which the second solution is added relative to the liquid surface of the first solution in the first container, In the aforementioned support state, the optical unit is for irradiating a mixture of the thawed first solution, the second solution added to the first solution, the biological sample, and the indicator with excitation light, and for detecting detection light including fluorescence generated in the mixture as a result of the irradiation with the excitation light. Equipped with, The first additive unit is fitted with a second container containing the second solution and has a first nozzle for dispensing the second solution from the second container into the first solution. The first adjustment unit adjusts the position of the second solution relative to the liquid surface in the height direction by moving the first nozzle along the height direction intersecting the liquid surface. Measuring device.

3. The first adjustment unit moves the first nozzle along the height direction such that, when adding the second solution to the first solution, the distance of the outlet of the second solution in the second container from the liquid surface in the height direction is 1 mm or less in the range where the outlet does not come into contact with the liquid surface. The measuring device according to claim 1 or 2.

4. In the aforementioned support state, a blower is provided for supplying air to the first container, The first container includes a light-transmitting section that transmits the excitation light and the detection light, The blower supplies the air to the surface of the light-transmitting portion in the supported state. The measuring device according to claim 1 or 2.

5. The aforementioned optical unit is An optical filter for selectively transmitting the detection light from the mixture that is incident at an incident angle of a predetermined angle or less, A lens for focusing the detection light that has passed through the optical filter, A detector that detects the detected light focused by the lens, Having, The measuring device according to claim 1 or 2.

6. In the aforementioned support state, a second adjustment unit is provided for adjusting the position of adding the biological sample to the first solution in the first container, The second additive unit is fitted with a third container containing the biological sample and has a second nozzle for discharging the biological sample from the third container to the first container. The second adjustment unit adjusts the placement position of the biological sample in the height direction by moving the second nozzle along the height direction. The measuring device according to claim 1 or 2.

7. The second adjustment unit moves the second nozzle along the height direction so that the outlet for the biological sample in the third container is located in the first solution. The measuring device according to claim 6.

8. The second additive unit repeatedly performs the following actions when adding the biological sample to the first solution: discharging the biological sample from the third container using the second nozzle and introducing the first solution into the third container using the second nozzle. The measuring device according to claim 7.

9. A measurement method for measuring the properties of a biological sample using a measuring device, A first step involves supporting a first container, which contains a first solution that is frozen and contains an indicator that reacts with components generated from a biological sample, on the support part of the measuring device. A second step is performed after the first step, in which the first solution in the first container is thawed using the heater of the measuring device, A third step involves attaching a second container, which contains a second solution containing a stimulant that activates the function of the biological sample, to the first nozzle of the measuring device. A fourth step is performed after the second and third steps, in which the position of adding the second solution relative to the liquid surface of the first solution in the first container is adjusted. A fifth step is to add the second solution to the first solution in the first container after the fourth step, A sixth step is to add the biological sample to the first solution in the first container after the second step, A seventh step is performed, following the fifth and sixth steps, by the optical unit of the measuring device, in which excitation light is irradiated onto the first solution and the mixture of the biological sample added to the first solution and the second solution, and detection light including fluorescence generated in the mixture as a result of the irradiation with the excitation light is performed. In the fourth step, the first adjustment unit of the measuring device adjusts the addition position of the second solution relative to the liquid surface in the height direction by moving the first nozzle along the height direction intersecting the liquid surface of the first solution. Measurement method.

10. A measurement method for measuring the properties of a biological sample using a measuring device, A first step involves supporting a first container, which contains a frozen first solution for diluting the biological sample, on the support of the measuring device. A second step is performed after the first step, in which the first solution in the first container is thawed using the heater of the measuring device, A third step involves attaching a second container, which contains a second solution containing a stimulant that activates the function of the biological sample, to the first nozzle of the measuring device. A fourth step is performed after the second and third steps, in which the position of adding the second solution relative to the liquid surface of the first solution in the first container is adjusted. A fifth step is to add the second solution to the first solution in the first container after the fourth step, A sixth step is performed after the second step, in which an indicator that reacts with the biological sample and components generated from the biological sample is added to the first solution in the first container, A seventh step is performed, following the fifth and sixth steps, by the optical unit of the measuring device, irradiating the mixture of the first solution, the biological sample added to the first solution, the indicator, and the second solution with excitation light, and detecting the detection light including fluorescence generated in the mixture as a result of the irradiation with the excitation light. In the fourth step, the first adjustment unit of the measuring device adjusts the addition position of the second solution relative to the liquid surface in the height direction by moving the first nozzle along the height direction intersecting the liquid surface of the first solution. Measurement method.

11. In the fourth step, when the second solution is added to the first solution, the first adjustment unit of the measuring device moves the first nozzle along the height direction so that the distance from the liquid surface in the height direction of the outlet of the second solution in the second container is 1 mm or less in the range in which the outlet does not come into contact with the liquid surface. The measurement method according to claim 9 or 10.

12. In the second step, when thawing the first solution, the blower of the measuring device is used to supply air to the surface of the light-transmitting portion in the first container that transmits the excitation light and the detection light. The measurement method according to claim 9 or 10.

13. Prior to the sixth step, an eighth step is performed in which a third container containing the biological sample is attached to the second nozzle of the measuring device, A ninth step, performed after the eighth step and before the sixth step, involves adjusting the position in which the biological sample is added to the first solution in the first container, Equipped with, In the ninth step, the second adjustment unit of the measuring device adjusts the position in which the biological sample is added to the first solution in the height direction by moving the second nozzle along the height direction. The measurement method according to claim 9 or 10.

14. In the ninth step, the second adjustment unit of the measuring device moves the second nozzle along the height direction so that the outlet for the biological sample in the third container is located in the first solution. The measurement method according to claim 13.

15. In the sixth step, when adding the biological sample to the first solution, the process of discharging the biological sample from the third container using the second nozzle and introducing the first solution into the third container using the second nozzle is repeated. The measurement method according to claim 14.

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