Allergic dermatitis prevention and treatment agents

Abstract

The objective is to provide novel preventive and therapeutic agents for allergic dermatitis. [Solution] The technical features of the present invention for solving the above problems are as follows. 1. An antipruritic agent for immediate-type allergies containing lycoperosides and / or tomato seed extract as active ingredients. 2. The above-mentioned lycoperoside is characterized in that the lycoperoside is lycoperoside H, as described in 1. above, as an antipruritic agent for itching caused by immediate-type allergies. 3. An immediate-type allergy reaction inhibitor containing lycoperoside H and / or tomato seed extract as active ingredients.

Description

Technical Field

[0001] The present invention relates to a preventive and therapeutic agent for allergic dermatitis. The present invention is widely used in foods, drugs, cosmetics, external skin preparations, and the like.

Background Art

[0002] Tomato (Solanum lycopersicum) is a plant that is eaten daily worldwide and is sold as juice or supplements because it contains abundant components such as vitamin C, lycopene, and GABA. As functions of tomatoes, a blood pressure-lowering effect and a cholesterol-lowering effect have been reported. In 2004, new saponins called esculeosides were identified from tomatoes (Non-Patent Document 1 and Non-Patent Document 2), and among them, esculeoside A has been found to have an improving effect on hyperlipidemia and arteriosclerosis in mice (Non-Patent Document 3). However, all of these are findings in tomato fruits, and there are few reports limited to tomato seeds.

[0003] In addition, since dryness of the skin is a typical symptom of allergic dermatitis, maintaining the water content of the skin leads to alleviation of allergic dermatitis symptoms. In the skin, multiple moisturizing factors work, and typical factors include filaggrin and ceramide. It has been found that these are significantly reduced in the skin of atopic dermatitis patients, and it is considered that the dryness of the skin, which is an allergic dermatitis symptom, can be alleviated by increasing the filaggrin and ceramide content in the skin.

[0004] The biosynthetic pathway of sphingolipids is well-studied. First, as the initial step in sphingolipid synthesis, the non-essential amino acid L-serine and palmitoyl-CoA are used as starting materials. The serine palmitoyltransferase enzyme (SPT) condenses the palmitoyl-CoA and the non-essential amino acid L-serine, and ceramide is synthesized in the stratum spinosum through a four-step process. Then, sphingomyelin synthase converts it to sphingomyelin, which accumulates in the stratum granulosum, and subsequently, acidic sphingomyelinase synthesizes ceramide again in the stratum corneum (the pathway on the right in Figure 6).

[0005] Furthermore, ceramide synthesized in the stratum spinosum is converted to glucosylceramide by glucosylceramide synthase and accumulates in the stratum granulosum, where it is then synthesized again in the stratum corneum by glucocerebrosidase (the pathway on the left in Figure 6).

[0006] Furthermore, there are 10 types of ceramides (EOS, NS, NP, EOH, AS, AP, AH, NH, EOP), and among these, a deficiency in ceramides (EOS), (NP), and (AP) is said to have a particularly strong impact on dry skin, sensitive skin, and atopic dermatitis.

[0007] Here, ceramides (EOS, NS, EOH, AS, AP) are produced via the left pathway shown in Figure 6, which involves obtaining glucosylceramide. In contrast, the pathway on the right, which obtains sphingomyelin and produces ceramide, only produces ceramide (NS) and (AS). In particular, ceramide NP, which is involved in alleviating skin dryness caused by allergic dermatitis, is produced only through the pathway shown on the left (Patent Document 1).

[0008] [Non-Patent Document 1] Fujiwara S. et al., Tetrahedron, 60, 4915-4920 (2004). [Non-Patent Document 2] Ono M. et al., Chem. Pharm. Bull., 54(2), 237-239 (2006). [Non-Patent Document 3] Nohara T., J. Trad. Med., 27, 217-224 (2010). [Patent Document 1] Japanese Patent Publication No. 2017-105741 [Disclosure of the Invention] [Problems that the invention aims to solve]

[0009] Against the above background, the inventors evaluated the effectiveness of tomato seed extract and lycoperosides against allergic dermatitis and found that they have an allergic skin improving effect. Furthermore, they found that tomato seed extract and lycoperosides have a filaggrin expression promoting effect and a moisturizing effect, thus completing the present invention. In other words, the present invention aims to provide a novel agent for the prevention and treatment of allergic dermatitis. [Means for solving the problem]

[0010] The technical features of the present invention for solving the above problems are as follows. 1. An antipruritic agent for immediate-type allergies containing lycoperosides and / or tomato seed extract as active ingredients. 2. The above-mentioned lycoperoside is characterized in that the lycoperoside is lycoperoside H, as described in 1. above, as an antipruritic agent for itching caused by immediate-type allergies. 3. An immediate-type allergy reaction inhibitor containing Lycoperoside H and / or tomato seed extract as active ingredients. 4. A humectant containing lycoperosides and / or tomato seed extract as active ingredients. 5. A filaggrin gene expression promoter containing lycoperosides and / or tomato seed extract as active ingredients. 6. A ceramide synthase-3 expression promoter containing lycoperosides and / or tomato seed extract as active ingredients. 7. The agent according to items 4 to 6 above, characterized in that the lycoperoside is at least one of lycoperoside A and lycoperoside H. 8. A GCS gene expression enhancer comprising at least one of tomato seed extract, lycoperoside A, and lycoperoside H as an active ingredient. 9. A GBA gene expression promoter comprising at least one of tomato seed extract, lycoperoside A, and lycoperoside H as an active ingredient. 10. An SMS gene expression inhibitor comprising tomato seed extract and at least one of lycoperoside H as active ingredients. 11. A ceramide NP production promoter containing lycoperoside H as the active ingredient. 12. An allergic dermatitis preventive and therapeutic agent containing one of the agents described in any one of items 1 to 11 above as an active ingredient. 13. A preventive and therapeutic agent for allergic dermatitis containing lycoperosides and / or tomato seed extract as active ingredients. [Effects of the Invention]

[0011] According to the present invention, lycoperosides, which are saponins found only in tomato seeds and not in the fruit pulp, and tomato seed extract have an effect of suppressing itching caused by immediate-type allergies. Therefore, these components are useful as preventive and therapeutic agents for allergic dermatitis. Furthermore, among the lycoperosides mentioned above, lycoperoside H is particularly advantageous in suppressing itching caused by immediate-type allergies. Furthermore, lycoperoside H and / or tomato seed extract have an effect of suppressing immediate-type allergies. In addition, lycoperosides and tomato seed extracts have a moisturizing effect, a promoting effect on filaggrin gene expression, and also a promoting effect on the expression of ceramide synthase-3. Thereby, they can be used as agents for preventing and treating allergic dermatitis. Furthermore, lycoperosides and / or tomato seed extracts not only suppress immediate-type allergy, but also have an improving effect on dermatitis caused by dry skin, and thus are also useful as agents for improving dermatitis caused by dry skin. Thereby, in order to promote the production of ceramide in the left pathway in FIG. 6, it promotes the production of ceramide EOS, NP, AP, particularly ceramide NP, which is said to have a particularly strong effect on preventing skin dryness, sensitive skin, atopic dermatitis, etc. As described above, in the present invention, lycoperosides and tomato seed extracts are useful as agents for preventing and treating allergic dermatitis.

Brief Description of the Drawings

[0012] [Figure 1] It is a graph showing the effects of tomato seed extract and lycoperoside H on a mouse itching model. [Figure 2] It is a graph showing the effects of tomato seed extract and lycoperoside H on the mouse ear pinna PCA reaction. [Figure 3] It is a graph showing the effects of tomato seed extract and lycoperosides on filaggrin gene expression. [Figure 4] It is a graph showing the effects of tomato seed extract and lycoperosides on the expression of ceramide synthase-3 (CerS3) gene. [Figure 5] It is a graph showing the effects of tomato seed extract and lycoperosides on TEWL. [Figure 6] It is a diagram showing the action of tomato seed extract in the stratum corneum ceramide synthesis pathway. White arrows indicate the effects of tomato seed extract. [Figure 7]A graph showing the effect of tomato seed extract and lycoperosides on GCS gene expression. [Figure 8] A graph showing the effect of tomato seed extract and lycoperosides on GBA gene expression. [Figure 9] A graph showing the effect of tomato seed extract and lycoperosides on SMS gene expression. [Figure 10] A graph showing the effect of tomato seed extract and lycoperosides on ASM gene expression. [Figure 11] A graph showing the effect of tomato seed extract and lycoperosides on the amount of stratum corneum ceramide.

Best Mode for Carrying Out the Invention

[0013] The present invention will be described in detail below. The prophylactic and therapeutic agent for allergic dermatitis of the present invention is characterized by containing lycoperosides as an active ingredient. Further, the above lycoperosides are not particularly limited, and examples include lycoperoside A, lycoperoside H, etc. In addition, only one kind may be used, or two or more kinds may be used in combination. Lycoperoside A is a saponin represented by the following chemical formula (1).

Chemical

Chemical

[0014] The method for obtaining lycoperoside A and lycoperoside H is not particularly limited, but a method of extracting and purifying from plants is particularly preferred. When extracting lycoperoside A and lycoperoside H from plants, the raw material is not particularly limited, but tomatoes are especially preferred because they contain high concentrations of lycoperoside A and lycoperoside H. Furthermore, when obtaining lycoperoside A and lycoperoside H from tomatoes, the tomato seeds are used as the source. This is because lycoperoside A and lycoperoside H are not found in the pulp or skin of the tomato, but only in the seeds.

[0015] Furthermore, when solvent extraction is performed as the extraction method, examples of extraction solvents for obtaining the extract include water, lower monohydric alcohols (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (glycerin, propylene glycol, 1,3-butylene glycol, etc.), lower esters (ethyl acetate, etc.), hydrocarbons (benzene, hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, dipropyl ether, etc.), acetonitrile, etc., and one or more of these can be used.

[0016] Examples of preferred extraction methods include using aqueous ethanol or aqueous methanol at a concentration of 0-100% (v / v), extracting at room temperature or under heating for 1-10 hours, and then filtering.

[0017] As a purification method, the tomato seed extract obtained by the above method can be sequentially partitioned with ethyl acetate and n-butanol, and then lycoperoside A and lycoperoside H can be obtained by silica gel chromatography, column chromatography, etc.

[0018] As a specific method for obtaining lycoperoside A and lycoperoside H from tomato seeds, the method described in the examples herein is preferred.

[0019] The allergic dermatitis preventive and therapeutic agent of the present invention can be used as an ingredient in various food and beverage compositions. Examples of food and beverages include general foods such as confectionery (gum, candy, caramel, chocolate, cookies, snacks, jelly, gummies, tablets, etc.), noodles (soba, udon, ramen, etc.), dairy products (milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, and beverages (juice, coffee, tea, carbonated drinks, sports drinks, etc.), as well as health foods (tablets, capsules, etc.), nutritional supplements (nutritional drinks, etc.), functional foods, and foods for specified health uses. The allergic dermatitis preventive and therapeutic agent of the present invention can be appropriately incorporated into these food and beverages.

[0020] These food and beverage compositions can contain various ingredients depending on their type. For example, they can use food ingredients such as glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, carrageenan, casein, gelatin, pectin, agar, B vitamins, nicotinamide, calcium pantothenate, amino acids, calcium salts, colorants, flavorings, and preservatives.

[0021] Specifically, the allergic dermatitis preventive and therapeutic agent can be spray-dried or freeze-dried together with powdered cellulose, and then easily incorporated into food and beverages (such as instant foods) by processing it into powder, granules, tablets, or a solution. Alternatively, the allergic dermatitis preventive and therapeutic agent can be dissolved in, for example, oil, ethanol, glycerin, or a mixture thereof to form a liquid, which can then be added to beverages or solid foods. If necessary, it can also be mixed with a binder such as gum arabic or dextrin to form a powder or granules, which can then be added to beverages or solid foods.

[0022] When applying the allergic dermatitis prevention and treatment agent of the present invention to food and beverages, the amount added is preferably such that the total content of the active ingredient relative to the food and beverage is 1 to 20 wt%, since the main purposes are health maintenance and beauty.

[0023] The allergic dermatitis preventive and therapeutic agent of the present invention may be used as a material for pharmaceutical compositions (including pharmaceuticals and quasi-drugs). It can be manufactured by appropriately blending the allergic dermatitis preventive and therapeutic agent of the present invention with raw materials for pharmaceutical formulations. Examples of formulation raw materials that can be incorporated into the allergic dermatitis preventive and therapeutic agent of the present invention include excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cocoa butter, hydrogenated vegetable oil, kaolin, talc, etc.), binders (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), and disintegrants (sodium alginate, agar). Examples of additives include sodium bicarbonate, calcium carbonate, sodium lauryl sulfate, monoglyceride stearate, starch, lactose, acacia powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers (quaternary ammonium bases, sodium lauryl sulfate, etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), and lubricants (refined talc, stearate, polyethylene glycol, etc.).

[0024] The method of administering the allergic dermatitis preventive and therapeutic agent of the present invention can generally be oral administration in the form of tablets, pills, soft or hard capsules, granules, powders, granules, or liquids, but parenteral administration is also possible. When administered parenterally, it can be administered in solution form, or with the addition of dispersants, suspensions, stabilizers, etc., by local tissue administration, intradermal, subcutaneous, intramuscular, or intravenous injection. It may also be administered in the form of suppositories or other preparations.

[0025] The dosage may vary depending on the method of administration, the patient's condition, and the patient's age, but typically, adults can be administered 0.5 to 5000 mg of the active ingredient per day, and children can usually be administered 0.5 to 3000 mg. The mixing ratio of allergy dermatitis preventive and therapeutic agents can be adjusted as appropriate depending on the dosage form, but generally, when administered orally or via mucosal absorption, it is appropriate to use approximately 0.3 to 15.0 wt%, and when administered parenterally, approximately 0.01 to 10 wt%. Note that the dosage varies depending on various conditions, so in some cases a smaller amount than the above may be sufficient, and in other cases it may be necessary to administer a larger amount.

[0026] The allergic dermatitis preventive and therapeutic agent of the present invention can also be expected to have an allergic dermatitis preventive and therapeutic effect when used as a topical skin preparation (including cosmetics, pharmaceuticals, and quasi-drugs). Examples of topical skin preparations that may contain the allergic dermatitis preventive and therapeutic agent of the present invention include lotions, soaps, facial cleansers, bath additives, creams, lotions, toners, colognes, shaving creams, shaving lotions, cosmetic oils, sunscreens, face powders, foundations, perfumes, face masks, nail creams, nail polish, nail polish removers, eyebrow pencils, blushes, eye creams, eyeshadows, mascaras, eyeliners, lipsticks, lip balms, shampoos, conditioners, hair dyes, dispersions, and cleansing agents. Examples of pharmaceuticals or quasi-drugs that may contain the allergic dermatitis preventive and therapeutic agent of the present invention include ointments, creams, and topical solutions.

[0027] In addition to the allergic dermatitis preventive and therapeutic agent according to the present invention, the above-described form of topical skin preparation may contain ingredients commonly used in cosmetics, quasi-drugs, and other topical skin preparations, such as oils, higher alcohols, fatty acids, UV absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, etc., to the extent that they do not impair the allergic dermatitis preventive and therapeutic effect. Examples are listed below, but the present invention is not limited to these examples.

[0028] (1) Examples of oil content Ester-based oil phase components: Glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diethyl adipate Sopropyl, Isoarachil Neopentanoate, Caprylic / Capric Triglyceride, Trimethylolpropane Tri-2-ethylhexanoate, Trimethylolpropane Triisostearate, Pentaerythritol Tetra-2-ethylhexanoate, Cetyl Caprylate, Decyl Laurate, Hexyl Laurate, Decyl Myristate, Myristyl Myristate, Cetyl Myristate, Stearyl Stearate, Decyl Oleate, Cetyl Ricinoleate, Isostearyl Laurate, Isotridecyl Myristate, Isocetyl Myristate, Iso Tearyl, Isocetyl Palmitate, Isostearyl Palmitate, Octyl Stearate, Isocetyl Stearate, Isodecyl Oleate, Octyldodecyl Oleate, Octyldodecyl Linoleate, Isopropyl Isostearate, Cetostearyl 2-Ethylhexanoate, Stearyl 2-Ethylhexanoate, Hexyl Isostearate, Ethylene Glycol Dioctanoate, Ethylene Glycol Dioleate, Propylene Glycol Dicaprate, Di(Capryl / Capric Acid)Propylene Glycol, Propylene Glycol Dicaprylate, Dicaprin Neopentyl glycol acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, dipropyl carbonate,Dialkyl (C12-18) carbonate, triisocetyl citrate, triisoarachil citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate Examples include ropil, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, and isostearyl 12-stearoylhydroxystearate. Hydrocarbon-based oil phase components include squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum, etc. Animal and vegetable oils and their hydrogenated oils, and naturally derived waxes: animal oils and their hydrogenated oils such as beef tallow, hydrogenated beef tallow, pork tallow, hydrogenated pork tallow, horse oil, hydrogenated horse oil, mink oil, orange roughy oil, fish oil, hydrogenated fish oil, egg yolk oil, avocado oil, almond oil, olive oil, cocoa butter, kiwi seed oil, apricot kernel oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil, perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, Examples include hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil, sunflower oil, grape seed oil, jojoba oil, hydrogenated jojoba oil, macadamia nut oil, meadowfoam oil, cottonseed oil, hydrogenated cottonseed oil, coconut oil, hydrogenated coconut oil, and other vegetable oils and their hydrogenated oils, beeswax, high-acid value beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax, montan wax, and other waxes. Silicone-based oil phase components include dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstearoxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic silicone, trimethylsiloxysilicate, silicone RTV rubber, etc. Examples of fluorine-based oil phase components include perfluoropolyethers, fluorine-modified organopolysiloxanes, fluorinated pitch, fluorocarbons, fluoroalcohols, and fluoroalkyl / polyoxyalkylene copolymerized organopolysiloxanes.

[0029] (2) Examples of higher alcohols Examples include lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, and octyldodecanol.

[0030] (3) Examples of fatty acids Examples include caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid, erucic acid, and 2-ethylhexanoic acid.

[0031] (4) Examples of UV absorbers Para-aminobenzoic acid, amyl para-aminobenzoate, ethyl dihydroxypropyl para-aminobenzoate, glyceryl para-aminobenzoate, ethyl para-aminobenzoate, octyl para-aminobenzoate, octyl dimethyl para-aminobenzoate, ethylene glycol salicylate, octyl salicylate, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl para-methoxycinnamate, 2-ethylhexyl para-methoxycinnamate, glyceryl mono-2-ethylhexanoate dipara-methoxycinnamate, isopropyl para-methoxycinnamate, diethanolamine para-methoxyhydrocinnamate salt, diisopropyl / diisopropyl cinnamic acid ester mixture, urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, di Examples include hydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, butylmethoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2-ethylhexyl-1-oxy)-1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenyl acrylate, phenylbenzimidazole sulfate, 3-(4-methylbenzylidene)camphor, isopropyldibenzoylmethane, 4-(3,4-dimethoxyphenylmethylene)-2,5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and their polymer derivatives and silane derivatives.

[0032] (5) Examples of powders and pigments Dyes such as Red No. 104, Red No. 201, Yellow No. 4, Blue No. 1, Black No. 401, lake dyes such as Yellow No. 4 AL Lake, Yellow No. 203 BA Lake, nylon powder, silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polymers such as polyethylene powder, yellow iron oxide, red iron oxide, black iron oxide, chromium oxide, carbon black, ultramarine, Prussian blue, zinc oxide, titanium dioxide, acid Examples include white pigments such as cerium oxide, extender pigments such as talc, mica, sericite, kaolin, and plate-like barium sulfate, pearl pigments such as titanium mica, metal salts such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, and magnesium silicate, inorganic powders such as silica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, and zinc undecylenate, bentonite, smectite, and boron nitride. There are no particular restrictions on the shape (spherical, rod-shaped, needle-shaped, plate-shaped, irregular shape, flake-shaped, spindle-shaped, etc.) and particle size of these powders. These powders may or may not have been pre-treated by conventional surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil treatment, N-acylated lysine treatment, polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment, etc.

[0033] (6) Examples of surfactants Anionic surfactants include fatty acid soaps, α-acyl sulfonates, alkyl sulfonates, alkylallyl sulfonates, alkylnaphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphates, alkylamide phosphates, alkylylalkyl taurates, N-acyl amino acid salts, POE alkyl ether carboxylates, alkyl sulfosuccinates, alkyl sulfoacetate sodium, acylated hydrolyzed collagen peptides, perfluoroalkyl phosphates, and the like. Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride, beheninamidepropyldimethylhydroxypropylammonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like. Amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amidesulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, and amideamine type. Nonionic surfactants include propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE glycerin fatty acid esters, POE alkyl ethers, POE fatty acid esters, POE hydrogenated castor oil, POE castor oil, POE-POP copolymers, POE-POP alkyl ethers, polyether-modified silicone lauric acid alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like. Examples of natural surfactants include lecithin, saponin, and sugar-based surfactants.

[0034] (7) Examples of polyhydric alcohols and sugars Examples include ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1,3-butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugars, sulfated trehalose, pullulan, etc. Chemically modified versions of these can also be used.

[0035] (8) Examples of polymers Acrylic acid ester / methacrylic acid ester copolymer (Plussize, manufactured by Go-O Chemical Co., Ltd.), vinyl acetate / crotonic acid copolymer (Resin 28-1310, manufactured by NSC Corporation), vinyl acetate / crotonic acid / vinyl neodecanate copolymer (28-2930, manufactured by NSC Corporation), methyl vinyl ether maleate half ester (Gantlets ES, manufactured by ISP Corporation), T-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (Rubymer, manufactured by BASF Corporation), vinylpyrrolidone / vinyl acetate / vinyl propionate copolymer (Rubiscol VAP, manufactured by BASF Corporation), vinyl acetate β / crotonic acid copolymer (Rubicet CA, BASF), vinyl acetate / crotonic acid / vinylpyrrolidone copolymer (Rubicet CAP, BASF), vinylpyrrolidone / acrylate copolymer (Rubiflex, BASF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxyvinyl polymer (Carbopol, BFGoodrich), acrylic acid / alkyl methacrylate copolymer (Pemulene, BF Examples include anionic polymer compounds such as those manufactured by Goodrich, amphoteric polymer compounds such as the amphoteric acetate of dialkylaminoethyl methacrylate polymer (Yukaformer, manufactured by Mitsubishi Chemical Corporation), octylacrylamide acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, manufactured by NSC), cationic polymer compounds such as the quaternary compound of vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, manufactured by ISP), methylvinylimidazolium chloride / vinylpyrrolidone copolymer (Rubicort, manufactured by BASF), and nonionic polymer compounds such as polyvinylpyrrolidone (Rubiscol K, manufactured by BASF), vinylpyrrolidone / vinyl acetate copolymer (Rubiscol VA, manufactured by BASF), vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (Copolymer 937, manufactured by ISP), and vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (Copolymer VC713, manufactured by ISP).Furthermore, naturally derived polymer compounds such as cellulose or its derivatives, keratin and collagen or their derivatives, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharides, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, acacia gum, crystalline cellulose, arabinogalactan, karaya gum, tragacanth gum, alginic acid, albumin, casein, curdlan, gellan gum, and dextran can also be suitably used.

[0036] (9) Examples of bioactive ingredients Bioactive ingredients include substances that provide some kind of bioactivity to the skin when applied to the skin. Examples include whitening ingredients, immune stimulants, anti-aging agents, UV protection agents, slimming agents, skin tightening agents, antioxidants, hair growth agents, hair tonics, moisturizers, blood circulation promoters, antibacterial agents, disinfectants, drying agents, cooling agents, warming agents, vitamins, amino acids, wound healing promoters, irritation relievers, analgesics, cell activators, enzyme components, etc. Examples of suitable ingredients include Angelica keiskei extract, avocado extract, Hydrangea macrophylla extract, Althaea officinalis extract, Arnica montana extract, Aloe vera extract, apricot extract, apricot kernel extract, Ginkgo biloba extract, Fennel extract, Turmeric extract, Oolong tea extract, Rosa multiflora extract, Echinacea japonica leaf extract, Scutellaria baicalensis extract, Phellodendron amurense extract, Coptis japonica extract, Barley extract, Hypericum perforatum extract, Lamium album extract, Nasturtium officinale extract, Orange extract, Dried seawater, seaweed extract, hydrolyzed elastin, hydrolyzed wheat powder, hydrolyzed silk, chamomile extract, carrot extract, Artemisia capillaris extract, licorice extract, carrot extract, Arctium moniliforme extract, cinchona extract, cucumber extract, guanosine, gardenia extract, bamboo grass extract, Sophora flavescens extract, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, black tea extract, yeast extract, burdock extract, corn Rice bran fermentation extract, rice germ oil, comfrey extract, collagen, lingonberry extract, asarum extract, bupleurum extract, umbilical cord extract, salvia extract, soapwort extract, bamboo extract, hawthorn extract, sansho extract, shiitake mushroom extract, rehmannia extract, lithospermum extract, perilla extract, linden extract, meadowsweet extract, peony extract, calamus root extract, birch extract, horsetail extract, ivy extract, hawthorn extract, ce Elderflower extract, yarrow extract, peppermint extract, sage extract, mallow extract, Cnidium officinale extract, Swertia japonica extract, soybean extract, jujube extract, thyme extract, tea extract, clove extract, cogongrass extract, citrus peel extract, angelica extract, calendula extract, peach kernel extract, spruce extract, Houttuynia cordata extract, tomato extract, natto extract, carrot extract, garlic extract, wild rose extract, hibiscus extract,Examples of extracts include Ophiopogon japonicus extract, parsley extract, honey, witch hazel extract, Parietaria trifolia extract, Isodon japonicus extract, bisabolol, loquat extract, coltsfoot extract, butterbur extract, Poria cocos extract, butcher's broom extract, grape extract, propolis, loofah extract, safflower extract, peppermint extract, linden extract, peony extract, hop extract, pine extract, horse chestnut extract, skunk cabbage extract, soapberry extract, lemon balm extract, peach extract, cornflower extract, eucalyptus extract, saxifrage extract, coix seed extract, mugwort extract, lavender extract, apple extract, lettuce extract, lemon extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract, etc. Furthermore, it contains biopolymers such as deoxyribonucleic acid, mucopolysaccharides, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, and hydrolyzed eggshell membrane; moisturizing ingredients such as amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidone carboxylate, betaine, whey, and trimethylglycine; oily ingredients such as sphingolipids, ceramides, phytosphingosine, cholesterol, cholesterol derivatives, and phospholipids; ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, and guaiazulene. Immunostimulants such as hydroxyl, hydrocholtisone, vitamins such as vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, and vitamin C ester, active ingredients such as allantoin, diisopropylamine dichloroacetate, and 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as tocopherol, carotenoids, flavonoids, tannins, lignans, and saponins, cell activators such as alpha-hydroxy acids and beta-hydroxy acids, and blood circulation promoters such as gamma-oryzanol and vitamin E derivatives. Wound-healing agents such as retinol and retinol derivatives, whitening agents such as arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, and glutathione, cepharanthine, licorice extract, capsicum tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivatives, calcium pantothenate, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol All, ethinylestradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, tacanal, camphor, salicylic acid, vanillyl nonyl acid, vanillyl nonanoate, piroctone olamine, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcinol, gamma-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormones, cantharis tincture, cyclosporine, zinc pyrithione, hydrocoltisone, minoxidil, polyoxyethylene sorbitan monostearate, peppermint oil,Examples include hair growth products containing Sasanishiki rice extract.

[0037] (10) Examples of antioxidants Examples include sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tol biguanide, nordihydroguaiaretinic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxytoluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoids, flavonoids, tannins, lignans, saponins, and plant extracts with antioxidant effects such as apple extract and clove extract.

[0038] (11) Example of Be Examples include purified water, ethanol, lower alcohols, ethers, LPG, fluorocarbons, N-methylpyrrolidone, fluoroalcohols, volatile linear silicones, and next-generation chlorofluorocarbons (CFCs).

[0039] The allergic dermatitis prevention and treatment agent of the present invention can be used as a raw material for food and beverage compositions, pharmaceutical compositions, and topical skin preparations. The raw materials that can be incorporated into these formulations are the same as those used in the aforementioned allergic dermatitis preventive and therapeutic agents, and the manufacturing and administration methods are also the same as those used for the allergic dermatitis preventive and therapeutic agents. [Examples]

[0040] The present invention will be described below based on examples. Example 1: Preparation of tomato seed extract, and isolation and identification of saponin components from tomato seeds. 367.7 g of dried tomato seeds were extracted with methanol (70°C, 2 hours) to obtain a methanol extract. The obtained methanol extract was dispersed in water and sequentially partitioned with ethyl acetate and n-butanol to obtain 0.63 g of ethyl acetate-soluble portion (ethyl acetate fraction), 2.41 g of n-butanol-soluble portion (butanol fraction), and 5.05 g of water-soluble portion (water fraction). The butanol fraction was used as the tomato seed extract for this example. The butanol fraction was subjected to silica gel chromatography (chloroform:methanol = 9:1 → 7:3 → chloroform:methanol:water = 6:4:1 → methanol) to obtain Fr. 1 to 6. Fr. 3 (1.1 g) was subjected to reverse-phase ODS column chromatography (methanol concentration 20% → 50% → 80% → 100%) to obtain Fr. 3-1 to Fr. 3-4. The saponin compounds lycoperoside A (chemical formula (1) above) (7.0 mg) and H (chemical formula (2) above) (7.5 mg) were isolated from Fr.3-3 (mg) by reverse-phase HPLC preparative sampling (Inertsil ODS-SP, 70% methanol). Each compound was identified by comparing its 1H-NMR (600 MHz) and 13C-NMR (150 MHz) spectra with literature values.

[0041] Test Example 1. Effects on a mouse pruritus model. ddY mice were orally administered tomato seed extract (500 mg / kg) or lycoperoside H (10 or 30 mg / kg). One hour later, 50 μL of 3% saline solution of compound 48 / 80 was subcutaneously administered to the dorsal neck to induce scratching behavior in response to itching caused by immediate-type allergies. The mice were observed for 30 minutes after the first scratching behavior following induction, and the number of scratches was measured. Diphenhydramine hydrochloride (30 mg / kg) was used as a positive control. The results are shown in Figure 1.

[0042] Results and Effects of the Example in Test Example 1 The experimental results, as shown in Figure 1, confirmed that the number of scratches decreased with the administration of tomato seed extract compared to the control group. Furthermore, a concentration-dependent reduction in the number of scratches was also observed with the administration of lycoperoside H. This confirms that lycoperoside H and tomato seed extract are effective as antipruritic agents for itching caused by allergies.

[0043] Test Example 2. Evaluation using mouse auricle PCA response. ddY mice were sensitized by intradermal administration of anti-DNP-IgE diluted 2000-fold with physiological saline into both auricles. Two days later, tomato seed extract (500 mg / kg) or lycoperoside H (10 or 30 mg / kg) was administered orally, and two hours later, 0.25 mL of a 0.25 mg solution of DNP-BSA and 0.5% Evans blue physiological saline solution was administered via tail vein to induce an antigen-antibody reaction. 3 The auricles of mice that were lethally killed by cervical dislocation at 0 minutes were removed, and the pigment leaked from the auricles was extracted. The absorbance at 620 nm was measured. The results are shown in Figure 2.

[0044] Results and Effects of the Examples in Test Example 2 The experimental results showed a significant suppression of pigment leakage in the groups administered to tomato seed extract (500 mg / kg) and lycoperoside H (10 mg / kg) (Figure 2). These results clearly demonstrate that tomato seed extract and lycoperoside H exhibit inhibitory effects against immediate-type allergic reactions.

[0045] Test Example 3. Moisturizing effect in a 3D model of human epidermis. Tomato seed extract, lycoperoside H, or lycoperoside A were added to the membrane side of human epidermal 3D cells. RNA was extracted from the tissue collected after 48 hours, and mRNA expression of the filaggrin gene and ceramide synthase-3 (CerS3), a gene involved in ceramide synthesis, was analyzed by real-time RT-PCR. The results are shown in Figure 3 (filaggrin gene) and Figure 4 (CerS3). TEWL was also measured over time from 24 to 72 hours of culture. The results are shown in Figure 5. In Figure 5, "TSE" represents tomato seed extract, "LycoA" represents lycoperoside A, and "LycoH" represents lycoperoside H.

[0046] Results and Effects of the Example in Test Example 3 The experimental results showed that real-time RT-PCR significantly increased filaggrin expression with the addition of tomato seed extract (10 μg / mL), lycoperoside H (3 and 10 μM), and lycoperoside A (1 μM) (Figure 3). On the other hand, the expression of ceramide synthase-3 (CerS3), a ceramide synthesis-related enzyme, significantly increased with the addition of tomato seed extract (1 and 3 μg / mL) and lycoperoside H (1, 3, and 10 μM) (Figure 4). Furthermore, TEWL measurements showed that after 72 hours of culture, TEWL was significantly lower in the groups treated with tomato seed extract (10 μg / mL), lycoperoside H (10 μM), and lycoperoside A (10 μM) compared to the control group (Figure 5). These results indicate that tomato seed extract and lycoperosides increase the skin's moisture retention capacity by increasing the expression of skin moisturizing factors.

[0047] Test Example 4. Evaluation of ceramide-related gene expression in a 3D model of human epidermis. Tomato seed extract, lycoperoside A, or lycoperoside H were added to the membrane side of human epidermal 3D cells. RNA was extracted from the tissue collected after 48 hours, and mRNA expression of genes involved in ceramide synthesis (CerS, GCS, GBA, SMS, ASM) was analyzed by real-time RT-PCR. In addition, lipids were extracted from the stratum corneum isolated from the tissue after 7 days of culture, and stratum corneum ceramide was quantified by thin-layer chromatography (HPTLC). The results are shown in Figures 7 (GCS), 8 (GBA), 9 (SMS), 10 (ASM), and 11 (stratum corneum ceramide amount).

[0048] Results and Effects of the Examples in Test Example 4 The experimental results showed that real-time RT-PCR significantly increased the gene expression of glucosylceramide synthase (GCS) with the addition of tomato seed extract (3 μg / mL), lycoperoside A (3 μM), and lycoperoside H (3 and 10 μM) (Figure 7). Similarly, the gene expression of β-glucocerebrosidase (GBA) also significantly increased with the addition of tomato seed extract (3 and 10 μg / mL), lycoperoside A (3 and 10 μM), and lycoperoside H (10 μM) (Figure 8). On the other hand, the gene expression of tomato seed extract phingomyelin synthase (SMS) significantly decreased with the addition of tomato seed extract (10 μg / mL) and lycoperoside H (1, 3, and 10 μM) (Figure 9). No significant effect of tomato seed extract, lycoperoside A, and lycoperoside H on acid sphingomyelinase (ASM) gene expression was observed (Figure 10). This confirms that they promote ceramide production in the pathway shown on the left in Figure 6. Furthermore, quantification of stratum corneum ceramides by HPTLC revealed that ceramide NP significantly increased with the addition of lycoperoside H (1 and 10 μM) (Figure 11). Although no significant effect was observed for other ceramide species, they showed a tendency to increase with the addition of tomato seed extract, lycoperoside A, and lycoperoside H.

[0049] Effects of the Examples Tomato seed extract and lycoperoside compounds were found to have an inhibitory effect on immediate-type allergic reactions and to alleviate skin dryness. Therefore, their effectiveness as preventive and therapeutic agents for allergic dermatitis was confirmed.

[0050] The following are examples of formulations of the allergic dermatitis preventive and therapeutic agent (tomato seed extract or lycoperosides) of the present invention, but these examples are not intended to limit the present invention. Example formulation 1: Chewing gum Sugar 52.0 wt% Gum base 20.0 Glucose 10.0 Starch syrup 16.0 Fragrance 0.5 Cherry blossom extract 0.5 Glucosylceramide 0.5 Allergic dermatitis prevention and treatment agent 0.5 100.0 wt%

[0051] Formula example 2: Gummy Reduced starch syrup 38.0 wt% Granulated sugar 20.0 Glucose 20.0 Gelatin 4.7 Wednesday 9.68 Kiwi juice 4.0 Kiwi flavor 0.6 Dye 0.02 Cherry blossom extract 1.0 Glucosylceramide 1.0 Allergic dermatitis prevention and treatment agent 1.0 100.0 wt%

[0052] Formula example 3: Candy Sugar 50.0 wt% Starch syrup 33.0 water 14.2 organic acid 2.0 Fragrance 0.2 Cherry blossom extract 0.1 Glucosylceramide 0.1 Allergic dermatitis prevention and treatment agent 0.4 100.0 wt%

[0053] Example of formulation 4: Yogurt (hard / soft) Milk 41.5 wt% Skim milk powder 5.8 Sugar 8.0 Agar 0.15 Gelatin 0.1 Lactic acid bacteria 0.005 Cherry blossom extract 0.1 Glucosylceramide 0.1 Allergic dermatitis prevention and treatment agent 0.4 fragrance trace amount water residue 100.0 wt%

[0054] Formulation example 5: Soft drink Fructose-glucose liquid sugar 30.0 wt% Emulsifier 0.5 Strawberry seed extract 0.05 Glucosylceramide 0.05 Allergic dermatitis prevention and treatment agent 0.05 Fragrance (appropriate amount) Purified water remainder 100.0 wt%

[0055] Formulation Example 6: Soft Capsules Rice germ oil 86.0wt% Cherry blossom extract 0.5 Glucosylceramide 0.5 Emulsifier 12.0 Allergic dermatitis prevention and treatment agent 1.0 100.0 wt%

[0056] Formulation example 7: Tablets Lactose 53.0wt% Crystalline cellulose 30.0 Starch decomposition product 10.0 Cherry blossom extract 0.5 Glucosylceramide 0.5 Glycerin fatty acid ester 5.0 Allergic dermatitis prevention and treatment agent 1.0 100.0 wt%

[0057] Example of formulation 8: Granule oral preparation (pharmaceutical) Allergic dermatitis prevention and treatment agent 1.0 wt% Strawberry seed extract 0.5 Glucosylceramide 0.5 Lactose 30.0 Cornstarch 60.0 Crystalline cellulose 7.0 Polyvinylpyrrolidone 1.0 100.0 wt%

[0058] Formulation example 9: Tablet confectionery Sugar 75.4 wt% Glucose 19.0 Sucrose fatty acid ester 0.2 Strawberry seed extract 0.5 Glucosylceramide 0.5 Allergic dermatitis prevention and treatment agent 0.5 Purified water 3.9 100.0 wt%

[0059] Formula Example 10: Cat Food Corn 33.0 wt% Wheat flour 35.0 Meat meal 15.0 Beef tallow 8.9 Salt 1.0 Bonito extract 4.0 Strawberry seed extract 0.5 Glucosylceramide 0.5 Allergic dermatitis prevention and treatment agent 1.0 Taurine 0.1 Vitamins 0.5 Minerals 0.5 100.0 wt%

[0060] Formula Example 11: Dog Food Corn 30.0 wt% Meat (Chicken) 15.0 Defatted soybeans 10.0 Wheat flour 24.0 Bran 5.0 Cherry blossom extract 0.5 Glucosylceramide 0.5 Allergic dermatitis prevention and treatment agent 5.0 Animal fats and oils 8.9 Oligosaccharide 0.1 Vitamin 0.5 Minerals 0.5 100.0 wt%

[0061] Formula Example 12: Cosmetic Cream Squalane 20.0 wt% Beeswax 5.0 Refined jojoba oil 5.0 Glycerin 5.0 Glycerin monostearate 2.0 Polyoxyethylene (20) sorbitan Monosterate 2.0 Allergic dermatitis prevention and treatment agent 2.0 Preservative (appropriate amount) Fragrance (appropriate amount) Purified water remainder 100.0 wt%

[0062] Formula example 13: Lotion Ethanol 5.0 wt% Glycerin 2.0 1,3-Butylene glycol 2.0 Polyethylene oleyl ether 0.5 Sodium citrate 0.1 Citric acid 0.1 Allergic dermatitis prevention and treatment agent 0.1 Purified water remainder 100.0 wt%

[0063] Formula Example 14: Body Gel Macadamia nut oil 2.0 wt% Octyldodecyl myristate 10.0 Methylphenylpolysiloxane 5.0 Behenyl alcohol 3.0 Stearic acid 3.0 Batyl alcohol 1.0 Glyceryl monostearate 1.0 Polyoxyethylene sorbitol tetraoleate 2.0 Hydrogenated soybean phospholipid 1.0 Ceramide 0.1 Retinyl palmitate 0.1 Preservative (appropriate amount) Centella asiatica extract 1.0 Allergic dermatitis prevention and treatment agent 1.0 1,3-Butylene glycol 5.0 Purified water remainder 100.0 wt%

[0064] Formulation example 15: Emulsion Squalane 4.0 wt% Vaseline 2.5 Cetanol 2.0 Glycerin 2.0 Lipophilic glyceryl monostearate 1.0 Stearic acid 1.0 L-arginine 1.0 Allergic dermatitis prevention and treatment agent 0.5 Potassium hydroxide 0.1 fragrance trace amount Purified water remainder 100.0 wt%

[0065] Formula Example 16: Bath additive (liquid) Propylene glycol 50.0 wt% Ethanol 20.0 Sodium sulfate 5.0 Allergic dermatitis prevention and treatment agent 0.5 Lanolin 0.5 Avocado oil 0.5 Dye 1.5 Fragrance 22.0 100.0 wt% [Industrial applicability]

[0066] As described above, the present invention can provide a novel agent for the prevention and treatment of allergic dermatitis.

Claims

1. A filaggrin gene expression promoter comprising at least one selected from lycoperoside A, lycoperoside H, and tomato seed extract as an active ingredient, characterized in that the filaggrin gene expression promoter has an effect of improving dry skin caused by allergic dermatitis symptoms.

2. A ceramide synthase-3 gene expression promoter comprising tomato seed extract as an active ingredient, characterized in that the ceramide synthase-3 gene expression promoter has an effect of improving dry skin caused by allergic dermatitis symptoms.

3. A GCS gene expression promoter comprising at least one of tomato seed extract, lycoperoside A, and lycoperoside H as an active ingredient, characterized in that the GCS gene expression promoter has an effect of improving dry skin caused by allergic dermatitis symptoms.

4. A GBA gene expression promoter comprising at least one of tomato seed extract, lycoperoside A, and lycoperoside H as an active ingredient, characterized in that the GBA gene expression promoter has an effect of improving dry skin caused by allergic dermatitis symptoms.

5. An SMS gene expression inhibitor comprising at least one of tomato seed extract and lycoperoside H as active ingredients, characterized in that the SMS gene expression inhibitor has an effect of improving dry skin caused by allergic dermatitis symptoms.

6. A ceramide NP production promoter comprising lycoperoside H as an active ingredient, characterized in that the ceramide NP production promoter has an improving effect on dry skin caused by allergic dermatitis symptoms.

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