Methods for the stable supply of industrially used plants by the exchange (replacement) of cell nuclei (chromosomes) or the exchange (replacement) of differentiated functional cells such as embryonic cells, and methods for the conservation of endangered plant species.

Abstract

There have been challenges in realizing research themes in biology, such as ensuring a stable supply of agricultural products like grains and vegetables, as well as industrially used plants like garden trees and ornamental flowers, conserving endangered plant species, and restoring extinct plant species. [Solution] By applying genome editing technology, etc., the cell nuclei (chromosomes) of plants targeted for industrial use or academic research are exchanged (replaced) with the cell nuclei (chromosomes) of seeds, bulbs, and spores of closely related plant species, thereby causing the plants targeted for industrial use and academic research to germinate. This method will solve the problem.

Description

Technical Field

[0001] The present invention relates to applied technologies for cell-related technologies such as genome editing.

Background Art

[0002] Genome editing technology

[0003] Chromosome (DNA) extraction technology

Summary of the Invention

Problems to be Solved by the Invention

[0004] Plants germinate and grow from seeds, roots, spores, etc. Among plants used in industries such as for food and ornamental purposes, it has been difficult to stably secure seeds and spores with high added value, to conserve endangered plant species whose population numbers are decreasing in nature, and to restore extinct plant species which are important research themes in biology.

Means for Solving the Problems

[0005] By applying technologies such as genome editing, the cell nucleus (chromosome) of cells of plants targeted for industrial use or academic research is exchanged (replaced) with the cell nucleus (chromosome) of embryo cells of seeds, bulbs, and spores of plants of their related species. The cell nucleus (chromosome) of cells of plants targeted for industrial use or academic research is transplanted into embryo cells in seeds, bulbs, and spores of plants of their related species. (At this time, the cell nucleus (chromosome) of the embryo cells of the plant to be transplanted is removed in advance.) (Since the cell nucleus (chromosome) of cells of plants targeted for industrial use or academic research is the same regardless of the cell type, the cell nucleus of an easily sampled cell is used.) By this exchange (replacement) of the cell nucleus (chromosome), it is possible to germinate plants (plants targeted for industrial use or academic research) that are not the original plants of the seeds, bulbs, and spores. Furthermore, similar results can be obtained by exchanging (replacing) entire cells that have the ability to differentiate into various cell types, such as embryonic cells, rather than exchanging (replacing) the cell nucleus (chromosomes) within the cell using the same method (it is possible to germinate plants that are different from the original plant, such as plants used for industrial purposes or academic research, from seeds, bulbs, or spores of closely related species). This method will solve the problem.

Claims

1. By applying genome editing technology and other techniques, the cell nucleus (chromosome) inside the cells of seeds, bulbs, and spores is extracted, and the cell nucleus (chromosome) of another plant (a closely related species) is transplanted to germinate the plant into which the cell nucleus (chromosome) has been transplanted. This method involves mass-producing the germination and cultivation of transplanted plants, thereby contributing to the stable supply of agricultural crops, the conservation of endangered plant species, and the restoration of extinct plant species, ultimately serving industrial purposes and academic research. This method involves applying genome editing technology to exchange (replace) the cell nuclei (chromosomes) within the cells of seed bulb spores of plants that are "not of high added value for industrial use" or "not of academic research value" with the cell nuclei (chromosomes) of plants that are "of high added value for industrial use" or "of academic research value," and then germinating and cultivating them to benefit industrial use and academic research. (Since the cell nuclei (chromosomes) of "plants with high added value for industrial use" and "plants valuable for academic research" are the same in all cells, cell nuclei (chromosomes) of easily obtainable cells will be used.) (When transplanting cell nuclei (chromosomes) to roots or rhizomes, unlike seeds or spores, there are many cells, so instead of extracting the cell nuclei (chromosomes), the cell nuclei (chromosomes) of the plant to be germinated are directly transplanted.)

2. A plant germinated using the method described in claim 1.

3. Services and businesses using the plants described in claim 2.