Composition containing amino acids for alleviating low-temperature stress

Abstract

The present application relates to a composition for alleviating low-temperature stress and / or a composition for promoting plant growth, which contains, as an active ingredient, one or more amino acids selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, threonine, arginine, proline, lysine, valine, and tryptophan, and uses thereof.

Description

[Technical Field]

[0001] [CROSS-REFERENCE TO RELATED APPLICATIONS] This application claims the benefit of priority based on Korean Patent Application No. 10-2023-0058742 dated May 4, 2023, and all contents disclosed in the documents of this Korean patent application are incorporated herein by reference.

[0002] The present application relates to amino acids having cold stress-reducing effects and / or uses thereof. [Background technology]

[0003] Climate change is becoming more volatile due to global warming, resulting in frequent occurrence of weather disasters such as extreme heat, droughts, cold waves, heavy rains, and typhoons. The resulting decline in crop productivity is a global issue, and preparations for countermeasures are urgently needed. Among unpredictable weather disasters, cold damage and sudden low temperatures not only inhibit crop growth but also prevent fertilization, which is closely related to reduced crop productivity. Rapid recovery of plants after cold damage is crucial to recovering from reduced productivity due to weather disasters, and for this reason, there is a high demand for products that can alleviate low-temperature stress.

[0004] In the current fertilizer market, there is a growing need for sustainable, environmentally friendly products that can replace chemical fertilizers and pesticides due to the deterioration of cultivation environments and the saturation of chemical fertilizer usage, and in response to this, products based on a variety of environmentally friendly substances have been released. Amino acids are essential for plant growth and are one of the main substances that can be used as raw materials for growth promoters that can replace chemical fertilizers. The present inventors have selected amino acids that have the effect of alleviating low-temperature stress, which can enable normal growth of crops in low-temperature stress environments and overcome yield reductions. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] US 2018-0014536 A1 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present application is to provide a composition for alleviating low-temperature stress, which contains an amino acid as an active ingredient.

[0007] Another object of the present application is to provide a composition for promoting plant growth, which contains the amino acid or the composition for alleviating low-temperature stress.

[0008] It is still another object of the present application to provide a fertilizer composition comprising the amino acid, the composition for alleviating low-temperature stress, and / or the composition for promoting plant growth.

[0009] It is still another object of the present application to provide an agricultural chemical composition comprising the amino acid, the composition for alleviating low-temperature stress, and / or the composition for promoting plant growth.

[0010] Another object of the present application is to provide a method for cultivating a plant, a method for promoting plant growth, a method for alleviating low-temperature stress in a plant, and / or a method for increasing low-temperature stress resistance in a plant, the method comprising the step of treating a plant with the amino acid, the composition for alleviating low-temperature stress, and / or the composition for promoting plant growth.

[0011] Another object of the present application is to provide a use of the amino acid for alleviating low-temperature stress and / or promoting plant growth.

[0012] Another object of the present application is to provide a use of the amino acid for producing a composition for alleviating low-temperature stress, a composition for promoting plant growth, a fertilizer composition, and / or an agrochemical composition. [Means for solving the problem]

[0013] This will be described in detail below. Meanwhile, each description and embodiment disclosed in this application may also be applied to each other description and embodiment. In other words, all combinations of the various elements disclosed in this application fall within the scope of this application. Furthermore, the specific descriptions set forth below should not be construed as limiting the scope of this application. Furthermore, those skilled in the art will recognize or ascertain, using no more than routine experimentation, numerous equivalents to the specific aspects of this application described herein. Furthermore, such equivalents are intended to be encompassed by this application.

[0014] The present application will now be described in more detail.

[0015] amino acid The amino acids provided in this application include arginine (Arg), histidine (His), lysine (Lys), aspartic acid (Asp), glutamic acid (Glu), serine (Ser), threonine (Thr), asparagine (Asn), glutamine (Gln), cysteine ​​(Cys), glycine (Gly), proline (Pro), alanine (Ala), valine (Val), isoleucine (Ile), leucine (Leu), methionine (Met), and phenylalanine (Phe). , tyrosine (Tyr), and tryptophan (Trp). The amino acid may be one or more (e.g., 1 or more, 2 or more, 3 or more, 4 or more, 5 or more, 6 or more, 7 or more, 8 or more, 9 or more, 10 or more, 11 or more, 12 or more, 13 or more, 14 or more, 15 or more, 16 or more, 17 or more, 18 or more, or 19 or more, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20) amino acids or salts thereof.

[0016] As used herein, the term "amino acid" refers to an amino acid, a salt thereof, or all of these, and may refer to an amino acid, a salt thereof, or all of these unless otherwise specified.

[0017] As used herein, "one or more" amino acids may include amino acids used alone or two or more amino acids used in combination.

[0018] As used herein, the term "single" amino acid may mean that the amino acid is not used in combination with any other type of amino acid.

[0019] As used herein, "two or more" amino acids can include the use of a combination of two or more, three or more, four or more, etc. amino acids.

[0020] In the present application, the term "salt of an amino acid" refers to a physiologically acceptable salt among salts in which a cation and an anion are bound by electrostatic attraction, e.g., a salt applicable to plant cultivation. For example, the salt may be one selected from the group consisting of metal salts, salts with organic bases, salts with inorganic acids, and salts with organic acids. For example, the metal salt may be one or more selected from the group consisting of alkali metal salts (such as sodium salts and potassium salts), alkaline earth metal salts (such as calcium salts, magnesium salts and barium salts), and aluminum salts; the salt with an organic base may be one or more selected from the group consisting of salts with triethylamine, pyridine, picoline, 2,6-lutidine, ethanolamine, diethanolamine, triethanolamine, cyclohexylamine, dicyclohexylamine, N,N-dibenzylethylenediamine, and the like; the salt with an inorganic acid may be one or more selected from the group consisting of salts with hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, phosphoric acid, and the like; and the salt with an organic acid may be one selected from the group consisting of salts with formic acid, acetic acid, trifluoroacetic acid, phthalic acid, fumaric acid, oxalic acid, tartaric acid, maleic acid, citric acid, succinic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, and the like.

[0021] In one example, the salt of histidine may be histidine hydrochloride (His-HCl), the salt of glutamic acid may be sodium glutamate (monosodium glutamate, MSG), and the salt of lysine may be lysine hydrochloride (Lys-HCl).

[0022] The present application has confirmed that the above amino acids, when used alone or in combination of two or more types, have the effect of alleviating low-temperature stress and / or promoting plant growth, and proposes the low-temperature stress alleviating activity and / or plant growth promoting activity of the following amino acids.

[0023] In one example, the amino acid may be any one selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, glutamic acid, phenylalanine, serine, and threonine.

[0024] In one example, the amino acid may be any one selected from the group consisting of alanine, glutamine, histidine, isoleucine, phenylalanine, serine, and threonine.

[0025] In one example, the amino acid may be any one selected from the group consisting of asparagine, isoleucine, leucine, serine, and threonine.

[0026] For example, the amino acid may be any one selected from the group consisting of alanine, histidine, glutamine, and glutamic acid.

[0027] When the amino acid is any one selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, glutamic acid, phenylalanine, serine, and threonine, the effect of reducing low temperature stress and / or the effect of promoting plant growth are more excellent.

[0028] In one example, the amino acid is (1) a combination of two or more selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, and threonine; or (2) (i) one or more selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, and threonine, and (ii) a combination of one or more selected from the group consisting of arginine and proline.

[0029] In one example, the amino acid is (1) a combination of two or more selected from the group consisting of alanine, glutamine, histidine, isoleucine, glutamic acid, phenylalanine, serine, and threonine; or (2) (i) one or more selected from the group consisting of alanine, glutamine, histidine, isoleucine, glutamic acid, phenylalanine, serine, and threonine, and (ii) a combination of one or more selected from the group consisting of arginine and proline.

[0030] In this case, for example, the combination of amino acids may not include threonine and histidine.

[0031] In one example, the amino acid is (1) a combination of two or more selected from the group consisting of alanine, glutamine, histidine, and glutamic acid; or (2) It may be a combination of (i) one or more selected from the group consisting of alanine, glutamine, histidine, and glutamic acid, and (ii) one or more selected from the group consisting of arginine and proline.

[0032] In one example, the amino acid is (1) alanine; and a combination of one or more selected from the group consisting of arginine, glutamine, glutamic acid, and proline; (2) arginine; and a combination of one or more selected from the group consisting of histidine, glutamine, and glutamic acid; (3) histidine; and a combination of one or more selected from the group consisting of glutamine and glutamic acid; (4) a combination of glutamine and proline; and (5) It may be a combination of one or more selected from the group consisting of combinations of glutamic acid and proline.

[0033] In one example, the amino acid is (1) alanine and arginine, glutamine, glutamic acid, or proline; (2) a combination of arginine and histidine, glutamine, or glutamic acid; (3) a combination of histidine and glutamine or glutamic acid; (4) a combination of glutamine and proline; or (5) It may be a combination of glutamic acid and proline.

[0034] In one example, the amino acids may be one or more combinations selected from the group consisting of a combination of alanine and glutamic acid, a combination of alanine and proline, a combination of arginine and histidine, a combination of arginine and glutamine, a combination of histidine and glutamine, a combination of histidine and glutamic acid, and a combination of glutamic acid and proline.

[0035] In one example, the amino acids may be a combination of alanine and glutamic acid, a combination of alanine and proline, a combination of arginine and histidine, a combination of arginine and glutamine, a combination of histidine and glutamine, a combination of histidine and glutamic acid, or a combination of glutamic acid and proline.

[0036] The amino acids are (1) a combination of two or more selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, and threonine; or (2) When the combination of (i) one or more selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, and threonine, and (ii) one or more selected from the group consisting of arginine and proline is used, the effect of reducing low-temperature stress and / or the effect of promoting plant growth are more excellent.

[0037] In one example, the amino acids may be a combination of two or more selected from the group consisting of arginine, histidine, glutamic acid, lysine, valine, and tryptophan.

[0038] In one example, the amino acid is (1) arginine and histidine; and (2) It may be a combination of one or more selected from the group consisting of glutamic acid, lysine, valine, and tryptophan.

[0039] In one example, the amino acid is (1) arginine, histidine, and glutamic acid; and (2) It may be a combination of one or more selected from the group consisting of lysine, valine, and tryptophan.

[0040] In one example, the amino acid may be one selected from the group consisting of the following (1) to (5): (1) a combination of arginine, histidine, and glutamic acid; (2) a combination of arginine, histidine, and lysine; (3) a combination of arginine, histidine, glutamic acid, and lysine; (4) a combination of arginine, histidine, glutamic acid, and valine; and (5) A combination of arginine, histidine, glutamic acid, valine, and tryptophan.

[0041] In one example, the amino acids may be a combination of arginine, histidine, and glutamic acid.

[0042] In one example, the amino acids may be a combination of arginine, histidine, and lysine.

[0043] In one example, the amino acids may be a combination of arginine, histidine, glutamic acid, and lysine.

[0044] In one example, the amino acids may be a combination of arginine, histidine, glutamic acid, and valine.

[0045] In one example, the amino acids may be a combination of arginine, histidine, glutamic acid, valine, and tryptophan.

[0046] When the amino acids are a combination of two or more selected from the group consisting of arginine, histidine, glutamic acid, lysine, valine, and tryptophan, the effect of reducing low-temperature stress and / or the effect of promoting plant growth are more excellent.

[0047] When two of the amino acids are used in combination, the mixing ratio of each amino acid may be, by weight, 1:0.01 to 100, 1:0.1 to 10, 1:4 to 4:1, 1:4 to 3:1, 1:4 to 2:1, 1:4 to 1:1, 1:4 to 1:2, 1:4 to 1:3, 1:3 to 4:1, 1:3 to 3:1, 1:3 to 2:1, 1:3 to 1:1, 1:3 to 2:1, 1:2 to 4:1, 1:2 to 3:1, 1:2 to 2:1, 1:2 to 1:1, 1:1 to 4:1, 1:1 to 3:1, 1:1 to 2:1, or 1:1, but is not limited thereto.

[0048] When two or more kinds of the amino acids are used in combination, the mixing ratio of each amino acid is, based on 1 part by weight of arginine, histidine may be 0.1 to 5 parts by weight, 0.1 to 4 parts by weight, 0.1 to 3 parts by weight, 0.1 to 2 parts by weight, 0.1 to 1.5 parts by weight, 0.1 to 1.2 parts by weight, 0.1 to 1 part by weight, 0.5 to 5 parts by weight, 0.5 to 4 parts by weight, 0.5 to 3 parts by weight, 0.5 to 2 parts by weight, 0.5 to 1.5 parts by weight, 0.5 to 1.2 parts by weight, 0.5 to 1 part by weight, 1 to 5 parts by weight, 1 to 4 parts by weight, 1 to 3 parts by weight, 1 to 2 parts by weight, 1 to 1.5 parts by weight, or 1 to 1.2 parts by weight; Glutamic acid may be 0.1 to 5 parts by weight, 0.1 to 4 parts by weight, 0.1 to 3 parts by weight, 0.1 to 2.67 parts by weight, 0.1 to 2.5 parts by weight, 0.1 to 2.4 parts by weight, 0.1 to 2 parts by weight, 0.1 to 1 part by weight, 0.5 to 5 parts by weight, 0.5 to 4 parts by weight, 0.5 to 3 parts by weight, 0.5 to 2.67 parts by weight, 0.5 to 2.5 parts by weight, 0.5 to 2.4 parts by weight, 0.5 to 2 parts by weight, 0.5 to 1 part by weight, 1 to 5 parts by weight, 1 to 4 parts by weight, 1 to 3 parts by weight, 1 to 2.67 parts by weight, 1 to 2.5 parts by weight, 1 to 2.4 parts by weight, or 1 to 2 parts by weight, Lysine may be 0.1 to 5 parts by weight, 0.1 to 4 parts by weight, 0.1 to 3 parts by weight, 0.1 to 2.67 parts by weight, 0.1 to 2.5 parts by weight, 0.1 to 2.4 parts by weight, 0.1 to 2 parts by weight, 0.1 to 1 part by weight, 0.5 to 5 parts by weight, 0.5 to 4 parts by weight, 0.5 to 3 parts by weight, 0.5 to 2.67 parts by weight, 0.5 to 2.5 parts by weight, 0.5 to 2.4 parts by weight, 0.5 to 2 parts by weight, 0.5 to 1 part by weight, 1 to 5 parts by weight, 1 to 4 parts by weight, 1 to 3 parts by weight, 1 to 2.67 parts by weight, 1 to 2.5 parts by weight, 1 to 2.4 parts by weight, or 1 to 2 parts by weight. Valine may be 0.01 to 5 parts by weight, 0.01 to 4 parts by weight, 0.01 to 3 parts by weight, 0.01 to 2 parts by weight, 0.01 to 1 part by weight, 0.01 to 0.5 parts by weight, 0.01 to 0.4 parts by weight, 0.01 to 0.1 part by weight, 0.1 to 5 parts by weight, 0.1 to 4 parts by weight, 0.1 to 3 parts by weight, 0.1 to 2 parts by weight, 0.1 to 1 part by weight, 0.1 to 0.5 parts by weight, or 0.1 to 0.4 parts by weight, The amount of tryptophan may be, but is not limited to, 0.01 to 5 parts by weight, 0.01 to 4 parts by weight, 0.01 to 3 parts by weight, 0.01 to 2 parts by weight, 0.01 to 1 part by weight, 0.01 to 0.5 parts by weight, 0.01 to 0.4 parts by weight, 0.01 to 0.1 part by weight, 0.1 to 5 parts by weight, 0.1 to 4 parts by weight, 0.1 to 3 parts by weight, 0.1 to 2 parts by weight, 0.1 to 1 part by weight, 0.1 to 0.5 parts by weight, or 0.1 to 0.4 parts by weight.

[0049] Compositions and Methods In one aspect, after confirming that the amino acid has the effect of alleviating low-temperature stress and / or promoting plant growth, a composition for alleviating low-temperature stress and / or a composition for promoting plant growth containing the amino acid is provided.

[0050] Another aspect provides the use of said amino acids for the relief of cold stress and / or for the promotion of plant growth.

[0051] Another aspect provides a use of the amino acid for producing a composition for alleviating low-temperature stress and / or a composition for promoting plant growth.

[0052] The amino acids are as described above.

[0053] In one example, the composition can include one or more of the amino acids.

[0054] In one example, the composition may be an aqueous amino acid solution, which may consist of the amino acid and water.

[0055] As used herein, "low temperature stress" may refer to damage caused by low temperatures to plant growth, flowering time, seed production, and the like.

[0056] In the present application, the low temperature stress includes 20°C or less, 19°C or less, 18°C ​​or less, 17°C or less, 16°C or less, 15°C or less, 13°C or less, 12°C or less, 11°C or less, 10°C or less, -50 to 20°C, -50 to 17°C, -50 to 15°C, -50 to 13°C, -50 to 10°C, -50 to 7°C, -50 to 5°C, -50 to 3°C, -50 to 0°C, - 40~20℃, -40~17℃, -40~15℃, -40~13℃, -40~10℃, -40~7℃, -40~5℃, -40~3℃, -40~0℃, -30~20℃, -30~17℃, -30~15℃, -30~13℃, -30~10℃, -30~7℃, -30~5℃, -30~3℃, -30~0℃, -20~20℃, - 20~17℃, -20~15℃, -20~13℃, -20~10℃, -20~7℃, -20~5℃, -20~3℃, -20~0℃, -10~20℃, -10~17℃, -10~15℃, -10~13℃, -10~10℃, -10~7℃, -10~5℃, -10~3℃, -10~0℃, 0~20℃, 0~17℃, 0~15℃ , 0 to 13°C, 0 to 10°C, 0 to 7°C, 0 to 5°C, 0 to 3°C, 5 to 20°C, 5 to 17°C, 5 to 15°C, 5 to 13°C, 5 to 10°C, 5 to 7°C, 10 to 20°C, 10 to 17°C, 10 to 15°C, 10 to 13°C, 13 to 20°C, 13 to 17°C, or 13 to 15°C, but is not limited thereto.

[0057] In the present application, when a composition for alleviating low temperature stress containing the amino acid is applied to a plant in which low temperature stress has been induced, it can have the effect of alleviating low temperature stress.

[0058] For example, plants induced by low temperature stress may exhibit increased chlorosis, reduced plant growth, phenotypic changes such as inhibition of cell division, inhibition of plant growth, inhibition of photosynthesis, accelerated senescence, inhibition of fruit formation and / or development, changes in metabolic products leading to the accumulation of anthocyanins, and accumulation of reactive oxygen species (ROS).

[0059] In one example, the reduction in plant growth may be a reduction in the fresh weight of the above-ground part, the above-ground length, the fresh weight of the underground part, and / or the underground length.

[0060] In one example, the composition for alleviating low temperature stress may reduce chlorosis, increase plant growth, or reduce the accumulation of anthocyanins or reactive oxygen species when applied to a plant in which low temperature stress has been induced.

[0061] In one example, the increased growth of the plant can be an increase in the fresh weight of the above-ground part, the above-ground length, the fresh weight of the underground part, and / or the underground length.

[0062] In one example, the plant growth-promoting composition may promote plant growth by reducing and / or preventing plant chlorosis, increasing plant growth, and / or reducing and / or preventing the accumulation of anthocyanins or reactive oxygen species when applied to plants in which low temperature stress has been induced or to plants in which low temperature stress has not been induced.

[0063] The concentration of the amino acid contained in the composition for alleviating low temperature stress or the composition for promoting plant growth may be, by weight, 0.001 to 99.99% (w / w), 0.001 to 99.9% (w / w), 0.001 to 99% (w / w), 0.001 to 90% (w / w), 0.001 to 80% (w / w), 0.001 to 70% (w / w), 0.001 to 60% (w / w), 0.001 to 50% (w / w), 0.001 to 40% (w / w), 0.001 to 30% (w / w), 0.001 to 20% (w / w), 0.001 to 1 0%(w / w), 0.001~5%(w / w), 0.001~1%(w / w), 0.001~0.5%(w / w), 0.001~0.05%(w / w), 0.001~0.005%(w / w), 0.01~99.99%(w / w), 0.01~99.9% (w / w), 0.01~99%(w / w), 0.01~90%(w / w), 0.01~80%(w / w), 0.01~70%(w / w), 0.01~60%(w / w), 0.01~50%(w / w), 0.01~40%(w / w), 0.01~30%(w) / w), 0.01 to 20% (w / w), 0.01 to 10% (w / w), 0.01 to 5% (w / w), 0.01 to 1% (w / w), 0.01 to 0.5% (w / w), 0.01 to 0.05% (w / w), 0.1 to 99.99% (w / w), 0.1 to 99.9% (w / w), 0.1 to 99% (w / w), 0.1 to 90% (w / w), 0.1 to 80% (w / w), 0.1 to 70% (w / w), 0.1 to 60% (w / w), 0.1 to 50% (w / w), 0.1 to 40% (w / w), 0.1 to 30% (w / w), 0.1 to 20%(w / w), 0.1~10%(w / w), 0.1~5%(w / w), 0.1~1%(w / w), 0.1~0.5%(w / w), 1~99.99%(w / w), 1~99.9%(w / w), 1~99%(w / w), 1~90%(w / w), 1~80% (w / w), 1~70%(w / w), 1~60%(w / w), 1~50%(w / w), 1~40%(w / w), 1~30%(w / w), 1~20%(w / w), 1~23.5%(w / w), 1~5%(w / w), 5~99.99%(w / w), 5~99.9%(w / w), 5~99%(w / w), 5~90%(w / w), 5~80%(w / w), 5~70%(w / w), 5~60%(w / w), 5~50%(w / w), 5~4 0%(w / w), 5~30%(w / w), 5~25%(w / w), 5~23.5%(w / w), 5~20%(w / w), 10~99.99%(w / w), 10~99.9%( w / w), 10~99%(w / w), 10~90%(w / w), 10~80%(w / w), 10~70%(w / w), 10~60%(w / w), 10~50%(w / w), 10~40%(w / w), 10~30%(w / w), 10~25%(w / w), 10~23.5%(w / w), 10~20%(w / w), 20~99.99%(w / w), 2 0~99.9%(w / w), 20~99%(w / w), 20~90%(w / w), 20~80%(w / w), 20~70%(w / w), 20~60%(w / w), 20~5 0%(w / w), 20~40%(w / w), 20~30%(w / w), 20~25%(w / w), 20~23.5%(w / w), 22~99.99%(w / w), 22~99 The concentration may be, but is not limited to, 22-99% (w / w), 22-90% (w / w), 22-80% (w / w), 22-70% (w / w), 22-60% (w / w), 22-50% (w / w), 22-40% (w / w), 22-30% (w / w), 22-25% (w / w), or 22-23.5% (w / w).

[0064] The composition for alleviating low temperature stress or the composition for promoting plant growth may be used after being appropriately diluted, for example, 1 to 10,000 times, 1 to 7,500 times, 1 to 5,000 times, 1 to 2,500 times, 1 to 1,000 times, 1 to 900 times, 1 to 800 times, 1 to 700 times, 1 to 600 times, 1 to 500 times, 1 to 400 times, 1 to 300 times, 1 to 200 times, 1 to 100 times, 10 to 10,000 times, 10 to 7,500 times, 10 to 5,000 times, 10 to 2,500 times, 10 to 1,000 times, 10 to 900 times, 10 to 800 times, 10 to 700 times, 10 to 600 times, 10 to 500 times, 10 to 400 times, 10 to 300 times, 10 to 200 times, 10 to 300 times, 10 to 400 times, 10 to 500 times, 10 to 400 times, 10 to 500 times, 10 to 400 times, 10 to 500 times, 10 to 600 times, 10 to 200 times, 10 to 10 ... ~100x, 50~10000x, 50~7500x, 50~5000x, 50~2500x, 50~1000x, 50~900x, 50~800x times, 50~700 times, 50~600 times, 50~500 times, 50~400 times, 50~300 times, 50~200 times, 50~100 times, 100~1000 times The composition may be diluted 0-fold, 100-7500-fold, 100-5000-fold, 100-2500-fold, 100-1000-fold, 100-900-fold, 100-800-fold, 100-700-fold, 100-600-fold, 100-500-fold, 100-400-fold, 100-300-fold, or 100-200-fold before use. In a specific example, the composition may be diluted 500-fold before use. The composition may be diluted with water or the like, but is not limited thereto.

[0065] When the composition for alleviating low temperature stress or the composition for promoting plant growth is diluted before use, the concentration of the amino acid contained in the composition may be 0.0001 to 5% (w / w), 0.0001 to 4% (w / w), 0.0001 to 3% (w / w), 0.0001 to 1% (w / w), 0.0001 to 0.5% (w / w), 0.0001 to 0.01% (w / w), 0.0001 to 0.05% (w / w), 0.0001 to 0.047% (w / w), 0.0001 to 0.01% (w / w), 0.0001 to 0.009% (w / w), 0.0001 to 0.00 8%(w / w), 0.0001~0.007%(w / w), 0.0001~0.006%(w / w), 0.0001~0.005%(w / w), 0.0005~5%(w / w), 0.0005~4%(w / w), 0.0005~3%(w / w), 0.0005~1%(w / w) , 0.0005~0.5%(w / w), 0.0005~0.01%(w / w), 0.0005~0.05%(w / w), 0.0005~0 .047%(w / w), 0.0005~0.01%(w / w), 0.0005~0.009%(w / w), 0.0005~0.008%(w / w), 0.0005~0.007%(w / w), 0.0005~0.006%(w / w), 0.0005~0.005%(w / w), 0.001~5%(w / w), 0.001~4%(w / w), 0.001~3%(w / w), 0.001~1%(w / w), 0.001~0 .5%(w / w), 0.001~0.047%(w / w), 0.001~0.01%(w / w), 0.001~0.05%(w / w), 0 .001~0.01%(w / w), 0.001~0.009%(w / w), 0.001~0.008%(w / w), 0.001~0.00 7%(w / w), 0.001~0.006%(w / w), 0.001~0.005%(w / w), 0.005~5%(w / w), 0.00 5~4%(w / w), 0.005~3%(w / w), 0.005~1%(w / w), 0.005~0.5%(w / w), 0.005~0. 01%(w / w), 0.005~0.05%(w / w), 0.005~0.01%(w / w), 0.005~0.009%(w / w), 0 .005~0.008%(w / w), 0.005~0.007%(w / w), 0.01~5%(w / w), 0.01~4%(w / w), 0.01~3%(w / w), 0.01~1%(w / w), 0.01~0.5%(w / w), 0.01~0.047%(w / w), 0.01~0.05%(w / w), 0.02~5%(w / w), 0.02~4%(w / w), 0.02~3%(w / w), 0.02~1%(w / w), 0.02~0.5%(w / w) ), 0.02~0.047%(w / w), 0.02~0.01%(w / w), 0.02~0.05%(w / w), 0.03~5%(w / w), 0.03~ 4%(w / w), 0.03~3%(w / w), 0.03~1%(w / w), 0.03~0.5%(w / w), 0.03~0.01%(w / w), 0.03 ~0.05%(w / w), 0.03~0.047%(w / w), 0.04~5%(w / w), 0.04~4%(w / w), 0.04~3%(w / w), 0 .04~1%(w / w), 0.04~0.5%(w / w), 0.04~0.01%(w / w), 0.04~0.05%(w / w), 0.04~0.047 The concentration may be, but is not limited to, 0.044-5% (w / w), 0.044-4% (w / w), 0.044-3% (w / w), 0.044-1% (w / w), 0.044-0.5% (w / w), 0.044-0.01% (w / w), or 0.044-0.05% (w / w).

[0066] As used herein, the term "plant" refers to all physical parts of a plant, including one or more parts selected from the group consisting of seeds, seedlings, saplings, roots, tubers, stems, stalks, foliage, and fruits.

[0067] The plant may be a plant in which cold stress has been induced, or a normal plant in which cold stress has not been induced.

[0068] The plants to which the composition for alleviating low temperature stress or the composition for promoting plant growth can be applied are not particularly limited, and may be, for example, one or more plants selected from the group consisting of Cucurbitaceae (family of Cucurbitaceae), Asteraceae (family of Asteraceae), Brassicaceae (family of Cruciferae), Fabaceae (family of Fabaceae), Rosaceae (family of Rosaceae), and Solanaceae (family of Solanaceae), but are not limited thereto.

[0069] The Cucurbitaceae family plants include plants of the genus Cucumis (e.g., cucumber (Cucumis sativus), Japanese melon (Cucumis melo ssp. agrestis var. makuw), melon (Cucumis melo), and white melon (Cucumis melo var. conomon.)), plants of the genus Citrullus (e.g., watermelon (Citrullus lanatus)), plants of the genus Cucurbita (e.g., pumpkin (Cucurbita moschata, Cucurbita maxima, Cucurbita ficifolia)), plants of the genus Luffa (e.g., Luffa cylindrica (L.) M. Roem.)), and plants of the genus Wax gourd (e.g., Luffa cylindrica (L.) M. Roem.). The plant may be, but is not limited to, a plant of the genus Momordica (for example, Momordica charantia), or the like.

[0070] The Asteraceae plant may be, but is not limited to, a plant of the genus Raphanus (e.g., Raphanus sativus var. Longipinnatus, Raphanus raphanistrum, Raphanus raphanistrum subsp. sativus, etc.), a plant of the genus Lactuca (e.g., Lactuca indica, Lactuca raddeana, Lactuca sativa, Lactuca serriola, Lactuca triangulata, etc.), etc.

[0071] The Brassicaceae family plants include plants of the genus Arabidopsis (e.g., Arabidopsis thaliana), plants of the genus Brassica (e.g., Brassica oleracea, Chinese cabbage (Brassica rapa subsp. pekinensis), common cabbage (Brassica carinata), mustard greens (Brassica juncea var. juncea), turnips (Brassica rapa), bok choy (Brassica rapa subsp. chinensis), cabbage (Brassica oleracea var. capitata), rapeseed (Brassica napus), mustard (Brassica campestris L.), black mustard (Brassica nigra), and collard greens (Brassica oleracea var. viridis), Chinese kale (Brassica oleracea Alboglabra Group), cauliflower (Brassica oleracea var. botrytis), Brussels sprouts (Brassica oleracea var. gemmifera), kohlrabi (Brassica oleracea Gongylodes Group), broccoli (Brassica oleracea var. italica), savoy cabbage (Brassica oleracea var. sabauda), palm flowers (Brassica oleracea var. botrytis), kale (Brassica oleracea var. sabellica), etc., but are not limited thereto.

[0072] The legume family (family of Fabaceae) plants include plants of the genus Phaseolus (e.g., Phaseolus vulgaris, Phaseolus lunatus, Phaseolus coccineus, etc.), plants of the genus Glycine (e.g., bean (or soybean) (Glycine max), Glycine soja, etc.), plants of the genus Pisum (e.g., Pisum sativum, etc.), plants of the genus Arachis (e.g., Arachis hypogaea, etc.), plants of the genus Euchresta (e.g., Euchresta japonica Hook. f. ex Regel, etc.), plants of the genus Atractylodes macrocarpa (e.g., Atractylodes macrocarpa (Atractylodes macrocarpa)) The plant may be, but is not limited to, a Rhynchosia plant (e.g., Rhynchosia volubilis Lour, Rhynchosia acuminatifolia Makino, etc.), a plant of the genus Lens (e.g., Lens culinaris, or lentil bean, etc.), etc.

[0073] The Rosaceae family may include, but is not limited to, plants of the Fragaria genus (e.g., strawberry (Fragaria x ananassa)), plants of the Rubus genus (e.g., Rubus crataegifolius), etc.

[0074] The Solanaceae family includes plants of the genus Solanum (e.g., potato (Solanum tuberosum), eggplant (Solanum melongena), naranjilla (Solanum quitoense), tamarillo (Solanum betaceum), sweet nightshade (Solanum dulcamara), tomato (Solanum lycopersicum), pepino (Solanum muricatum), etc.), plants of the genus Nicotiana (e.g., tobacco (Nicotina tabacum)), plants of the genus Capsicum (e.g., peppers (Capsicum annuum), specifically chili peppers (Capsicum annuum L. var. acuminatum), sweet or bell peppers (Capsicum annuum L. var. grossum), and conger eel peppers (cone peppers). pepper (Capsicum annuum L. var. conoides), cherry pepper (Capsicum annuum L. var. cerasiforme), red cluster pepper (Capsicum annuum L. var. fasciculatum), long pepper (Capsicum annuum L. var. longum), etc., but are not limited thereto.

[0075] The part of a plant to which the composition for alleviating low temperature stress or the composition for promoting plant growth can be applied is not particularly limited, and may be, for example, one selected from the group consisting of the whole plant, seeds, roots, leaves, stems, flowers, etc.

[0076] In one example, the composition can be applied directly to the roots of the plant, or to the soil or medium in which the plant grows.

[0077] Alternatively, in one example, the composition may be applied to the upper and / or lower surfaces of leaves by foliar fertilization or foliar spray, but is not limited thereto.

[0078] The composition for alleviating low temperature stress or the composition for promoting plant growth may be applied by coating on the surface of the soil around the plant, mixing into the soil, or irrigating, or by directly spraying or sprinkling on the stems, leaves, or branches of the plant, or by spraying or immersing on the seeds of the plant, but is not limited thereto.

[0079] The amount of the composition for alleviating low temperature stress or the composition for promoting plant growth to be applied to the plant at one time is about 0.1 to 1000 ml, 0.1 to 800 ml, 0.1 to 600 ml, 0.1 to 500 ml, 0.1 to 400 ml, 0.1 to 200 ml, 0.1 to 100 ml, 0.1 to 50 ml, 0.1 to 10 ml, 0.1 to 5 ml, 0.1 to 1 ml, 1 to 1000 ml, 1 to 800 ml, 1 to 600 ml, 1 to 500 ml, 1 to 400 ml, 1 to 200 ml, 1 to 100 ml, 1 to 50 ml, 1 to 10 ml, 1 to 5 ... ml, 10 to 1000 ml, 10 to 800 ml, 10 to 600 ml, 10 to 500 ml, 10 to 400 ml, 10 to 200 ml, 10 to 100 ml, 10 to 50 ml, 50 to 1000 ml, 50 to 800 ml, 50 to 600 ml, 50 to 500 ml, 50 to 400 ml, 50 to 200 ml, 50 to 100 ml, 100 to 1000 ml, 100 to 800 ml, 100 to 600 ml, 100 to 500 ml, 100 to 400 ml, or 100 to 200 ml, but is not limited thereto.

[0080] The composition for alleviating low temperature stress or the composition for promoting plant growth may be applied to a plant a total of 1 to 20 times, 1 to 15 times, 1 to 10 times, 1 to 9 times, 1 to 8 times, 1 to 7 times, 1 to 6 times, 1 to 5 times, 1 to 4 times, or 1 to 3 times, and may be applied at intervals of 1 day, 2 days, 3 days, 5 days, 7 days (or 1 week), 10 days, 14 days (or 2 weeks), 15 days, 20 days, 21 days (or 3 weeks), 25 days, 28 days (or 4 weeks), 30 days (or 1 month), 45 days, or 60 days (or 2 months), but is not limited thereto.

[0081] The composition for alleviating low temperature stress or the composition for promoting plant growth of the present application may be formulated in various dosage forms such as dustable powder, granule, wettable powder, water-soluble powder, water-dispersible granule, water-soluble granule, suspension emulsion, suspension concentrate, oil-in-water emulsion, flowable, or emulsifiable concentrate, and known additives, carriers, and the like can be used in the formulation.

[0082] Another aspect provides a fertilizer composition comprising the amino acid, the composition for alleviating low-temperature stress, and / or the composition for promoting plant growth.

[0083] Another aspect provides the use of said amino acids for the preparation of fertilizer compositions.

[0084] As used herein, the term "fertilizer" may refer to a substance that causes chemical changes in soil to aid in plant growth, a substance that provides nutrients to plants, etc. Fertilizers may include, but are not limited to, one or more elements selected from the group consisting of nitrogen, phosphorus, potassium, and calcium (e.g., one or more, two or more, three or more, or one, two, three, or four elements). Fertilizers may be classified as organic fertilizers or inorganic fertilizers. The organic fertilizers may be plant-based fertilizers or animal-based fertilizers. The plant-based fertilizers may be compost, manure, oil cakes such as sesame meal, rice bran, leaf mold, etc., and the animal-based fertilizers may be silkworm chrysalis meal, bone meal, meat meal, carcasses, manure, etc. The inorganic fertilizers may be simple fertilizers or complex fertilizers. The single fertilizer may be a nitrogenous fertilizer (containing urea, ammonium sulfate, ammonium nitrate, ammonium chloride, and / or calcium nitrogen, etc.), a phosphate fertilizer (containing superphosphate, triple superphosphate, fused phosphate fertilizer, and / or fused phosphorus, etc.), a potassium fertilizer (containing potassium chloride and / or potassium sulfate, etc.), a calcareous (calcium) fertilizer (containing lime, slaked lime, and / or calcium carbonate, etc.), a silicate fertilizer (containing calcium silicate, etc.), etc. The compound fertilizer may be a fertilizer containing two or more components selected from the group consisting of nitrogen, phosphorus, potassium, calcium, silica, etc.

[0085] The fertilizer may additionally contain inorganic elements such as sulfur, calcium, magnesia, boron, copper, zinc, manganese, iron, molybdenum, and the like.

[0086] The fertilizer may be used in the form of a solid fertilizer, a granular fertilizer, a powder fertilizer, a liquid fertilizer, a diluted liquid fertilizer, etc., and may be formulated into various dosage forms such as a powder form, a granular form, etc. In the formulation, known additives, carriers, etc. may be used.

[0087] In another aspect, there is provided an agricultural chemical composition comprising the amino acid, the composition for alleviating low-temperature stress, and / or the composition for promoting plant growth.

[0088] Another aspect provides the use of said amino acids for the preparation of pesticide compositions.

[0089] In this specification, pesticide means a fungicide, insecticide, herbicide, etc. used to control plants and animals such as bacteria, insects, mites, nematodes, viruses, and weeds that harm plants (including agricultural crops, trees, agricultural products, forest products, etc.), and can also mean a drug used to enhance or suppress the physiological functions of agricultural crops.

[0090] The pesticide may be any commonly known pesticide such as a fungicide, insecticide, acaricide, nematicide, herbicide, or plant growth regulator, without any limitation.

[0091] The fungicide may be, but is not limited to, inorganic copper agents such as Bordeaux mixture, copper oxychloride, copper hydroxide, etc.; organic copper agents such as oxine copper, prochloraz copper chloride, etc.; inorganic sulfur agents such as lime sulfur, sulfur, etc.; organic sulfur agents such as dithiocarbamate (mancozeb, thiram, etc.); quinones (dithianon), arylnitriles (chlorothalonil), etc.

[0092] The insecticide may be, but is not limited to, an organophosphate insecticide such as diazinon, acephate, chlorpyrifos, or fenthion; a carbamate insecticide such as methiocarb, thiodicarb, benfuracarb, or carbofuran; a Bt insecticide such as Bt aizawai or Bt kurstaki; a synthetic pyrethroid insecticide such as etofenprox; or a neonicotinoid insecticide such as dinotefuran, acetamiprid, or thiamethoxam.

[0093] The acaricide may be, but is not limited to, synthetic pyrethroids such as bifenthrin and acrinathrin, antibiotics such as milbemectin and abamectin, organotin compounds such as cytin, acytin and fenbutatin oxide, METI compounds such as acequinocyl, fenazaquin and pyridaben, and tetronic acid compounds such as spirodiclofen, spiromesifen and spirotetramate.

[0094] The nematicide may be, but is not limited to, imidiyaphos, cadusafos, fosthiazate, and the like.

[0095] The herbicide may be, but is not limited to, a glycine-based herbicide such as glyphosate, or a phosphonic acid-based herbicide such as glufosinate ammonium.

[0096] The plant growth regulator may be a plant hormone such as auxin, gibberellin, cytokinin, abscisic acid (ABA), ethylene, or brassinosteroid, or a non-hormonal plant growth regulator such as 1-methylcyclopropene, maleic hydrazide, mepiquat chloride, butralin, prohexadione calcium, nitrophenolate, chlorofenuron, daminozide, diniconazole, inabenfide, or iprobenfos, but is not limited thereto.

[0097] The additives used to formulate the pesticide composition are not particularly limited, and additives commonly used in pesticide formulations can be used. For example, in the case of a fluidizing agent, water, an antifoaming agent, etc. can be added; in the case of an emulsifiable concentrate, an organic solvent (oil-based solvent) can be added; and in the case of a water-dispersible granule or a water-soluble granule, water can be added during granule preparation and subsequently removed by drying. A carrier can be added to formulate dusts, granules, wettable powders, etc. The carrier is not particularly limited, and carriers commonly used in pesticide formulations can be used. Examples of carriers include calcium carbonate, calcium sulfate, ammonium sulfate, potassium sulfate, sodium sulfate, sodium benzoate, silicon dioxide, diatomaceous earth, apatite, talc, bentonite, pyrophyllite, clay, and joint soil.

[0098] In another aspect, there is provided a method for cultivating a plant, the method comprising the step of treating the plant with the amino acid, the composition for alleviating low-temperature stress, and / or the composition for promoting plant growth.

[0099] In another aspect, there is provided a method for promoting plant growth, comprising the step of treating a plant with the amino acid, the composition for alleviating low-temperature stress, and / or the composition for promoting plant growth.

[0100] In one example, the method can be characterized by alleviating cold stress in a plant.

[0101] In one example, the method can be characterized by increasing cold stress resistance in a plant.

[0102] In another aspect, there is provided a method for alleviating cold stress in a plant or a method for enhancing cold stress resistance in a plant, the method comprising treating the plant with the amino acid, the composition for alleviating cold stress, and / or the composition for promoting plant growth.

[0103] In the composition and / or method, the type and concentration of amino acids, applicable plants, treatment volume, treatment frequency, treatment interval, etc. are as described above. [Effects of the Invention]

[0104] By treating plants with a composition containing the amino acid of the present application, it has been confirmed that the composition has excellent effects of reducing cold stress in plants, increasing cold stress resistance in plants, and / or promoting plant growth. [Brief explanation of the drawings]

[0105] [Figure 1a] 1 is a graph showing the results of testing the efficacy of amino acids in reducing low-temperature stress, and showing the underground growth (root length) of Arabidopsis thaliana. [Figure 1b] 1 is a graph showing the results of testing the efficacy of amino acids in reducing low-temperature stress, and showing the underground growth (root length) of Arabidopsis thaliana. [Figure 2]This is a graph showing the results of testing the efficacy of amino acids in reducing cold stress, and the relative anthocyanin content (absorbance at 530 nm wavelength / gF W (gram Fresh Weight)). [Figure 3] This graph shows the results of testing the effectiveness of six amino acids (Ala, Arg, His, Gln, MSG, Pro) and combinations of two selected amino acids in mitigating cold stress, as well as the growth of Arabidopsis shoots (rosette diameter). Experimental groups that performed better than the cold stress-induced control group (DW) are shown with white bars, while experimental groups that demonstrated a synergistic effect of the two amino acids are shown with patterned bars. [Figure 4] This graph shows the relative anthocyanin content of six amino acids (Ala, Arg, His, Gln, MSG, and Pro) and combinations of two selected amino acids, as well as the results of testing their effectiveness in mitigating cold stress. Experimental groups that performed better than the cold-stressed control group (DW) are shown with white bars, while experimental groups that demonstrated a synergistic effect of the two amino acids are shown with patterned bars. [Figure 5] 1 is a graph showing the results of verifying the efficacy of the amino acid compositions (CJ1, CJ2, CJ3, CJ4, and CJ5) of the present application in alleviating low-temperature stress, illustrating the leaf length of cucumbers. [Figure 6] 1 is a graph showing the results of verifying the efficacy of the amino acid compositions (CJ1, CJ2, CJ3, CJ4, and CJ5) of the present application in alleviating low-temperature stress, illustrating the leaf width of cucumber. [Figure 7] 1 is a graph showing the results of verifying the efficacy of the amino acid compositions (CJ1, CJ2, CJ3, CJ4, and CJ5) of the present application in alleviating low-temperature stress, showing the fresh weight of cucumbers. DETAILED DESCRIPTION OF THE INVENTION

[0106] The present invention will be described in more detail below with reference to the following examples, which are merely for illustrative purposes and are not intended to limit the scope of the present invention.

[0107] Example 1. Selection of a single amino acid with excellent efficacy in alleviating low-temperature stress in Arabidopsis thaliana In order to select amino acids with excellent efficacy in alleviating low-temperature stress in Arabidopsis thaliana, we grew Arabidopsis thaliana and conducted the following experiment using growth inhibition and anthocyanin accumulation as indicators.

[0108] Arabidopsis seeds were disinfected in a 50% (v / v) H2O2 solution for 2 minutes, rinsed five times in distilled water (DW), and then dark-treated for 3 days at 4°C. Arabidopsis seeds were sown on plant growth medium (1 / 2 MS; Murashige and Skoog Medium) and grown in a plant growth chamber (temperature 23°C, humidity 60-65%, light 16h / dark 8h) for 7 days.

[0109] Arabidopsis seedlings grown for 7 days were transferred to low temperature stress conditions (temperature 13°C, humidity 60-65%, light 16h / dark 8h), and root length was measured every 3 days. After 7 days, the fresh weight of the aboveground parts was measured.

[0110] Experimental plants were subjected to low-temperature stress (13°C, 60-65% humidity, 16h light / 8h dark) and foliar treatments of 0.1% (w / w) amino acid solutions (asparagine, aspartic acid, glycine, histidine, isoleucine, monosodium glutamate, phenylalanine, serine, and threonine) were performed three times at 3-day intervals. Control plants were grown at normal temperatures (23°C, 60-65% humidity, 16h light / 8h dark) without low-temperature stress, and a control plant was treated with distilled water (DW). The results are shown in Figure 1a.

[0111] Meanwhile, the experiment was repeated using alanine, glutamine, histidine, and sodium glutamate in a manner substantially similar to that described above, and the fresh weight of the aboveground parts was measured. The results are shown in Figure 1b.

[0112] In addition, Arabidopsis seedlings grown for 7 days were transferred to low temperature stress conditions (temperature 13°C, humidity 60-65%, light conditions light 16h / dark 8h), and samples for anthocyanin measurement were obtained and stored in an ultra-low temperature freezer.

[0113] Experimental plants were subjected to low-temperature stress (13°C, 60-65% humidity, 16h light / 8h dark) and foliar treatments of 0.1% (w / w) aqueous solutions of amino acids (alanine, glutamine, histidine, isoleucine, leucine, methionine, phenylalanine, serine, and threonine) were performed three times at 3-day intervals. Control plants were grown at normal temperatures (23°C, 60-65% humidity, 16h light / 8h dark) without low-temperature stress, and a control plant was treated with distilled water (DW). The results are shown in Figure 2.

[0114] The anthocyanin extraction and measurement method is as follows:

[0115] Approximately 30-50 mg of frozen sample was transferred to an Eppendorf tube. Because the sample was stored in an ultra-low temperature freezer, liquid nitrogen was used for transportation, and care was taken to prevent melting during transfer. 1 ml of 99% methanol (MeOH) and 1% hydrochloric acid (HCl) solution was added to each tube and mixed thoroughly using a vortex mixer. The tube was then incubated in the dark for 24 hours. After 24 hours of incubation, the sample was centrifuged (4°C, max speed, 5 minutes), and approximately 200 μl of the supernatant was transferred to a 96-well microplate. Because the extracted anthocyanins are easily destroyed by light, the tube was kept in the dark until measurement.

[0116] The absorbance values ​​were measured at wavelengths of 530 nm and 657 nm using a spectrophotometer (BioTek SYNERGY H1 microplate reader), and the anthocyanin content of the samples was calculated using the following formula: Anthocyanin content = (A530-0.25)*A657 (A: Absorbace, A530: absorbance at 530 nm wavelength, A657: absorbance at 657 nm wavelength)

[0117] Example 1-1. Root growth The results of the Arabidopsis root growth experiment are shown in FIG. 1a and Table 1 below.

[0118] [Table 1]

[0119] As can be seen from Figure 1a and Table 1, the results of testing the efficacy of low-temperature stress mitigation showed that root growth in the control group, where low-temperature stress was induced, was significantly reduced by more than 50% compared to the normal control group. However, in the experimental groups in which low-temperature stress was induced and treated with asparagine (Asn), aspartic acid (Asp), glycine (Gly), histidine (His), isoleucine (Ile), monosodium glutamate (MSG), phenylalanine (Phe), serine (Ser), or threonine (Thr), root growth was restored compared to the low-temperature stress-induced control group.

[0120] Furthermore, the results of the Arabidopsis root growth experiment conducted again using alanine, glutamine, histidine, and sodium glutamate are shown in Figure 1b and Table 2 below.

[0121] [Table 2]

[0122] As can be seen from Figure 1b and Table 2, the effectiveness of four amino acids in alleviating cold stress was re-examined. The results showed that the control group in which cold stress was induced showed a significant reduction in root growth of more than 50% compared to the normal control group. However, in the experimental groups in which cold stress was induced and treated with alanine (Ala), glutamine (Gln), histidine (His), or monosodium glutamate (MSG), root growth was restored compared to the control group in which cold stress was induced.

[0123] Example 1-2. Anthocyanin content A typical phenotypic change in plants subjected to low temperature stress is anthocyanin accumulation. The anthocyanin content (Abs530 / g FW) was measured from samples obtained from the aboveground tissues of Arabidopsis thaliana, and the results are shown in Figure 2 and Table 3 below.

[0124] [Table 3]

[0125] As can be seen from Figure 2 and Table 3, the results of testing the efficacy of low-temperature stress mitigation showed that the anthocyanin content in the low-temperature stress-induced control group was significantly higher than that in the normal-grown control group, but in the experimental groups that were treated with alanine (Ala), glutamine (Gln), histidine (His), isoleucine (Ile), leucine (Leu), phenylalanine (Phe), serine (Ser), or threonine (Thr), the anthocyanin content was significantly lower than that in the low-temperature stress-induced control group. This is due to a decrease in the activity of the anthocyanin synthesis process caused by low-temperature stress, indicating excellent efficacy in mitigating low-temperature stress.

[0126] Taking the experimental results of Examples 1-1 and 1-2 together, it was confirmed that alanine (Ala), asparagine (Asn), aspartic acid (Asp), glutamine (Gln), glycine (Gly), histidine (His), isoleucine (Ile), leucine (Leu), glutamic acid (Glu), phenylalanine (Phe), serine (Ser), and threonine (Thr) have excellent effects on reducing low-temperature stress.

[0127] Example 2: Selection of amino acid combinations with excellent efficacy in alleviating low-temperature stress in Arabidopsis thaliana Experiments were conducted on individual amino acids and amino acid combinations to verify the synergistic effects between amino acids when two or more of the six amino acids, alanine (Ala), histidine (His), glutamine (Gln), monosodium glutamate (MSG), arginine (Arg), and proline (Pro), were combined for treatment.

[0128] After sowing, the plants were filled with soil (Sungro, Sunshine mix #5) and then dark-treated at 4°C for three days. They were grown in a plant growth chamber (temperature 23°C, humidity 60-65%, light 16h / dark 8h). 2.5-3 weeks after sowing, they were transferred to a low-temperature growth chamber (cold stress conditions, temperature 13°C, light 16h / dark 8h). Six hours after transfer to the low-temperature growth chamber, 1 ml of amino acid was applied to the leaves as a primary fertilizer, followed three days later by a secondary fertilizer. Seven days later, final growth measurements (leaf diameter and weight) and anthocyanin measurements were performed, and samples were collected and stored in a deep-freezer.

[0129] The control group was a group grown at normal temperatures (23°C, 60-65% humidity, 16 hours light / 8 hours dark) without any stress treatment, as in Example 1. Another group was treated with low-temperature stress (13°C, 60-65% humidity, 16 hours light / 8 hours dark) and distilled water (DW). The experimental groups were treated with low-temperature stress and foliar treatment three times at 3-day intervals with either (1) a 0.1% (w / w) aqueous solution of each of the six amino acids or (2) a 0.1% (w / w) aqueous solution of two amino acids (a total of 15 combinations) (0.05% (w / w) of the first amino acid + 0.05% (w / w) of the second amino acid).

[0130] Example 2-1. Growth of aboveground parts The results of the experiment on the growth of the above-ground parts of Arabidopsis thaliana are shown in FIG. 3 and Table 4 below.

[0131] [Table 4]

[0132] As can be seen from Figure 3 and Table 4, the control group in which cold stress was induced showed a significant decrease in shoot growth compared to the control group in which plants grew normally. However, in the experimental group in which cold stress was induced and six single amino acids were individually treated (Ala, Arg, His, Gln, MSG, Pro), shoot growth increased compared to the control group in which cold stress was induced, re-examining the efficacy of the six amino acids in alleviating cold stress.

[0133] In the experimental groups treated with two combinations of amino acids, the combination of alanine and arginine (Ala-Arg), the combination of alanine and glutamine (Ala-Gln), the combination of alanine and monosodium glutamate (Ala-MSG), the combination of arginine and monosodium glutamate (Arg-MSG), the combination of histidine and monosodium glutamate (His-MSG), the combination of glutamine and proline (Gln-Pro), and the combination of monosodium glutamate and proline (MSG-Pro) showed increased shoot growth compared to the control group in which cold stress was induced, confirming their effectiveness in alleviating cold stress (shown by the white bars in Figure 3).

[0134] In particular, the experimental groups treated with the combination of alanine and proline (Ala-Pro), arginine and histidine (Arg-His), arginine and glutamine (Arg-Gln), and histidine-glutamine (His-Gln) not only showed a cold stress-alleviating effect compared to the cold stress-induced control group, but also showed a greater cold stress-alleviating effect than the experimental groups treated with each amino acid alone, confirming the existence of a synergistic effect between the amino acids (shown by the patterned bars in Figure 3).

[0135] Example 2-2. Anthocyanin content The anthocyanin content of the samples obtained from the above-ground tissues of Arabidopsis thaliana was measured, and the results are shown in FIG. 4 and Table 5 below.

[0136] [Table 5]

[0137] As can be seen from Figure 4 and Table 5, the anthocyanin content in the control group where cold stress was induced was significantly increased compared to the control group where plants were grown normally. However, in the experimental groups where cold stress was induced and six single amino acids were treated alone (Ala, Arg, His, Gln, MSG, Pro), the anthocyanin content decreased compared to the control group where cold stress was induced. This reaffirmed the efficacy of the six amino acids in alleviating cold stress.

[0138] In the experimental groups treated with two combinations of amino acids, the combination of alanine and arginine (Ala-Arg) and the combination of arginine and monosodium glutamate (Arg-MSG) showed reduced anthocyanin content compared to the control group in which cold stress was induced, confirming their effectiveness in alleviating cold stress (shown by the white bars in Figure 4).

[0139] In particular, experimental groups treated with a combination of alanine and monosodium glutamate (Ala-MSG), alanine and proline (Ala-Pro), arginine and histidine (Arg-His), arginine and glutamine (Arg-Gln), histidine and glutamine (His-Gln), histidine and monosodium glutamate (His-MSG), and monosodium glutamate and proline (MSG-Pro) not only showed a cold stress-alleviating effect compared to the cold stress-induced control group, but also showed a greater cold stress-alleviating effect than experimental groups treated with each amino acid alone, confirming the existence of a synergistic effect between the amino acids (represented by patterned bars in Figure 4).

[0140] Taking the experimental results of Examples 2-1 and 2-2 together, it was confirmed that the combination of alanine and arginine (Ala-Arg), the combination of alanine and glutamine (Ala-Gln), the combination of alanine and monosodium glutamate (Ala-MSG), the combination of alanine and proline (Ala-Pro), the combination of arginine and histidine (Arg-His), the combination of arginine and glutamine (Arg-Gln), arginine and monosodium glutamate (Arg-MSG), the combination of histidine and glutamine (His-Gln), the combination of histidine and monosodium glutamate (His-MSG), the combination of glutamine and proline (Gln-Pro), and the combination of monosodium glutamate and proline (MSG-Pro) had excellent cold stress alleviation effects.

[0141] Example 3. Selection of amino acid combinations with excellent efficacy in alleviating low-temperature stress in cucumber Taking into consideration the synergistic and antagonistic effects of the amino acids that exhibit the efficacy of alleviating low-temperature stress shown in Examples 1 and 2, five amino acid compositions that exhibit the efficacy of alleviating low-temperature stress were designed as shown in Table 6 below.

[0142] Cucumber (Cucumis sativus) seeds were sown, and approximately three weeks later, the cucumber seedlings were transferred to a low-temperature stress condition (temperature 25°C, humidity 60%, 12 hours of light / temperature 10°C, humidity 60%, 12 hours of darkness) at the two-leaf stage to induce low-temperature stress.

[0143] The experimental groups were subjected to low temperature stress, and an amino acid aqueous solution containing the composition shown in Table 6 below was diluted 500-fold and applied as a primary fertilizer at a volume of 3 ml / plant one day after low temperature stress induction, followed by a total of three foliar fertilization applications at four-day intervals. Control groups included a group that was not subjected to low temperature stress and was grown at normal temperatures (25°C, 60-65% humidity, 12 hours of light / 12 hours of darkness), and a group that was subjected to low temperature stress and treated with distilled water (DW).

[0144] [Table 6]

[0145] The leaf length (cm), leaf width (cm) and fresh weight (g) of the cucumber were measured and are shown in FIGS. 5 to 7 and Tables 7 to 9.

[0146] [Table 7]

[0147] [Table 8]

[0148] [Table 9]

[0149] As can be seen from Figures 5 to 7 and Tables 7 to 9, the control group in which low temperature stress was induced showed significant decreases in leaf length, leaf width, and fresh weight compared to the control group that was grown normally. However, in the experimental groups in which low temperature stress was induced and treated with amino acid compositions (CJ1, CJ2, CJ3, CJ4, and CJ5), leaf length, leaf width, and fresh weight increased compared to the control group in which low temperature stress was induced, confirming that the five amino acid compositions have the effect of alleviating low temperature stress.

[0150] From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are illustrative in all respects and are not limiting. The scope of the present invention should be interpreted as including within the meaning and scope of the claims below, rather than the above detailed description, and all modifications and variations derived from the equivalent concepts thereof.

Claims

1. A composition for reducing low-temperature stress, comprising, as an active ingredient, any one amino acid selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, glutamic acid, phenylalanine, serine, and threonine.

2. 2. The composition for alleviating cold stress according to claim 1, wherein the amino acid is any one selected from the group consisting of alanine, glutamine, histidine, isoleucine, phenylalanine, serine, and threonine.

3. The composition for alleviating low temperature stress according to claim 1 , wherein the composition consists of the amino acid and water.

4. 2. The composition for alleviating low-temperature stress according to claim 1, wherein the concentration of the amino acid contained in the composition is 0.001 to 20% (w / w).

5. 2. The composition for alleviating low temperature stress according to claim 1, wherein the composition is applied to one or more plants selected from the group consisting of Cucurbitaceae, Asteraceae, Brassicaceae, Leguminosae, Rosaceae, and Solanaceae.

6. 2. The composition for reducing low temperature stress according to claim 1, which is applied to one or more plants selected from the group consisting of Cucumis, Melon, Cucurbita, Luffa, Wax gourd, Bitter gourd, Radish, Lactuca, Arabidopsis, Brassica, Phaseolus, Glycine, Pisum, Arachis, Pineapple, Scutellaria, Lentil, Fragaria, Rubus, Solanum, Nicotiana, and Capsicum.

7. A composition for alleviating cold stress, comprising the following amino acids as active ingredients: (1) A combination of two or more selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, and threonine; or (2) (i) one or more selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, and threonine, and (ii) a combination of one or more selected from the group consisting of arginine and proline.

8. The amino acid is (1) a combination of two or more selected from the group consisting of alanine, glutamine, histidine, isoleucine, glutamic acid, phenylalanine, serine, and threonine; or (2) (i) one or more selected from the group consisting of alanine, glutamine, histidine, isoleucine, glutamic acid, phenylalanine, serine, and threonine, and (ii) a combination of one or more selected from the group consisting of arginine and proline; The composition for alleviating low temperature stress according to claim 7.

9. The amino acid is (1) a combination of two or more selected from the group consisting of alanine, glutamine, histidine, and glutamic acid; or (2) (i) one or more selected from the group consisting of alanine, glutamine, histidine, and glutamic acid, and (ii) a combination of one or more selected from the group consisting of arginine and proline; The composition for alleviating low temperature stress according to claim 7.

10. The amino acid is (1) a combination of two selected from the group consisting of alanine, glutamine, histidine, and glutamic acid; or (2) (i) any one selected from the group consisting of alanine, glutamine, histidine, and glutamic acid, and (ii) any one combination selected from the group consisting of arginine and proline; The composition for alleviating low temperature stress according to claim 7.

11. The amino acid is (1) a combination of alanine and arginine, glutamine, glutamic acid, or proline; (2) a combination of arginine and histidine, glutamine, or glutamic acid; (3) a combination of histidine and glutamine or glutamic acid; (4) a combination of glutamine and proline; or (5) A combination of glutamic acid and proline. The composition for alleviating low temperature stress according to claim 7.

12. 8. The composition for alleviating low-temperature stress according to claim 7, wherein the concentration of the amino acid contained in the composition is 0.001 to 20% (w / w).

13. 8. The composition for alleviating low temperature stress according to claim 7, which is applied to one or more plants selected from the group consisting of Cucurbitaceae, Asteraceae, Brassicaceae, Leguminosae, Rosaceae, and Solanaceae.

14. 8. The composition for reducing low temperature stress according to claim 7, which is applied to one or more plants selected from the group consisting of Cucumis, Melon, Cucurbita, Luffa, Wax gourd, Bitter gourd, Radish, Lactuca, Arabidopsis, Brassica, Phaseolus, Glycine, Pisum, Arachis, Pineapple, Scutellaria, Lentil, Strawberry, Rubus, Solanum, Nicotiana, and Capsicum.

15. (1) arginine, histidine; and (2) A composition for reducing low-temperature stress, comprising, as an active ingredient, one or more amino acids selected from the group consisting of glutamic acid, lysine, valine, and tryptophan.

16. The composition for alleviating low-temperature stress according to claim 15, wherein the amino acid is one or more selected from the group consisting of the following (1) to (5): (1) a combination of arginine, histidine, and glutamic acid; (2) a combination of arginine, histidine, and lysine; (3) a combination of arginine, histidine, glutamic acid, and lysine; (4) a combination of arginine, histidine, glutamic acid, and valine; and (5) A combination of arginine, histidine, glutamic acid, valine, and tryptophan.

17. The composition contains, relative to 1 part by weight of arginine, 0.1 to 5 parts by weight of histidine; 0.1 to 5 parts by weight of glutamic acid; 0.1 to 5 parts by weight of lysine; 0.01 to 5 parts by weight of valine; or 16. The composition for alleviating cold stress according to claim 15, comprising 0.01 to 5 parts by weight of tryptophan.

18. The composition for alleviating low temperature stress according to claim 15, wherein the concentration of the amino acid contained in the composition is 5 to 50% (w / w).

19. 16. The composition for alleviating low temperature stress according to claim 15, wherein the composition is applied to one or more plants selected from the group consisting of Cucurbitaceae, Asteraceae, Brassicaceae, Leguminosae, Rosaceae, and Solanaceae.

20. 16. The composition for reducing low temperature stress according to claim 15, which is applied to one or more plants selected from the group consisting of Cucumis, Melon, Cucurbita, Luffa, Wax gourd, Bitter gourd, Radish, Lactuca, Arabidopsis, Brassica, Phaseolus, Glycine, Pisum, Arachis, Pineapple, Scutellaria, Lentil, Fragaria, Rubus, Solanum, Nicotiana, and Capsicum.

21. A fertilizer composition comprising the composition for alleviating low temperature stress according to any one of claims 1 to 20.

22. An agricultural chemical composition comprising the composition for alleviating low-temperature stress according to any one of claims 1 to 20.

23. A method for cultivating plants or promoting plant growth, comprising the step of treating plants with the composition for alleviating low-temperature stress according to any one of claims 1 to 20.

24. A plant growth-promoting composition containing, as an active ingredient, any one of the following amino acids 1) to 3): 1) any one amino acid selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, glutamic acid, phenylalanine, serine, and threonine; ２） (1) A combination of two or more selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, and threonine; or (2) (i) one or more selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, and threonine, and (ii) a combination of one or more selected from the group consisting of arginine and proline; or ３） (1) arginine, histidine; and (2) One or more amino acids selected from the group consisting of glutamic acid, lysine, valine, and tryptophan.

25. Use of any one of the following amino acids 1) to 3) for reducing low-temperature stress or promoting plant growth: 1) any one amino acid selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, glutamic acid, phenylalanine, serine, and threonine; 2) (1) A combination of two or more selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, and threonine; or (2) (i) one or more selected from the group consisting of alanine, asparagine, aspartic acid, glutamine, glycine, histidine, isoleucine, leucine, methionine, glutamic acid, phenylalanine, serine, and threonine, and (ii) a combination of one or more selected from the group consisting of arginine and proline; and 3) (1) arginine, histidine; and (2) One or more amino acids selected from the group consisting of glutamic acid, lysine, valine, and tryptophan.

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