Compositions and methods for skin cell regeneration

Abstract

To provide deep skin cell regeneration without undesirable side effects such as desquamation, dry skin, itching, and erythema. [Solution] A composition is provided that combines oxygenated water with other components. This composition is generally intended to assist in the treatment of damaged skin tissue and to penetrate transdermally through the user's skin to the dermis. More specifically, the skin tissue regeneration composition may contain 80-95% by mass of oxygenated water and collagen in a concentration of more than 0% by mass and 5% by mass or less. The skin tissue regeneration composition may also contain 80-95% by mass of oxygenated water and elastin in a concentration of more than 0% by mass and 5% by mass or less. The use of the composition for the treatment of damaged skin tissue is also provided.

Description

Technical Field

[0001] [Cross - reference to Related Applications] There are no applications to be cross - referenced.

[0002] The present invention generally relates to a composition for regenerating skin cells and a method of using the composition. More specifically, the present invention relates to a composition for regenerating skin cells containing oxygenated water containing hydrolyzed collagen and / or elastin, and its use.

Background Art

[0003] Regarding skin care for regenerating skin tissue, particularly skin care in the treatment of scarred or aged skin, conventional treatment methods often cause many undesirable side effects. For example, silicone gel may be used for treating scars. Since silicone gel usually forms a silicone layer on the outer layer of the skin, it merely forms a protective layer on the outer layer of the skin and does not treat the damaged or aged tissue located under the outer layer of the skin.

[0004] Other formulations are also used for treating the skin. For example, there are formulations containing retinol as an active ingredient. Such formulations can bring beneficial effects such as cell regeneration, keratin removal, and synthesis of dermal collagen. However, such formulations may cause undesirable side effects such as flaking, dry skin, itching, and erythema.

[0005] Therefore, there is a need for a composition and a method of using it to overcome the drawbacks of conventional treatment regimens by providing regeneration of deep - layer skin cells without the above - mentioned side effects.

Summary of the Invention

[0006] The above object and other objects of the present invention are generally achieved by providing a composition for regenerating deep - layer skin cells. This composition contains oxygenated water at a concentration of 80 - 95% by mass and collagen at a concentration exceeding 0% by mass and not exceeding 5% by mass.

[0007] The collagen may be marine collagen powder, hydrolyzed collagen, hydrolyzed marine collagen, or a combination thereof. The composition may also contain organosilicon in a concentration greater than 0% by mass and less than or equal to 5% by mass. The organosilicon may be silanetriol. The composition may further contain propanediol, hyaluronic acid, organosilicon, soluble collagen peptide, acrylates / C10-30 alkyl acrylate crosspolymer, benzyl alcohol, ethylhexylglycerin, tocopherol, (caprylic / capric acid) coconut alkyl, sodium gluconate, and sodium hydroxide, or a combination thereof. This composition may contain 90.8% by mass of oxygenated water, 0.70% by mass of acrylates / C10-30 alkyl acrylate crosspolymer, 0.75% by mass of sodium hydroxide, 1.00% by mass of benzyl alcohol, ethylhexylglycerin and tocopherol, 1.50% by mass of organosilicon and hyaluronic acid, 0.20% by mass of sodium gluconate, 2.00% by mass of propanediol, 2.00% by mass of sodium hyaluronate, 1.00% by mass of water and hydrolyzed collagen, and 0.05% by mass of (caprylic / capric acid) coconut alkyl.

[0008] This composition may further contain elastin in a concentration exceeding 0% by mass and not exceeding 5% by mass. This composition may be in a form selected from gel, cream, liquid, spray, and mask. This composition may be in the form of a cream and may contain oxygenated water in a concentration of 20-25% by mass.

[0009] Another aspect of the present invention provides a composition for regenerating deep skin cells. This composition contains 80 to 95% by volume of oxygenated water and elastin in a concentration of more than 0% by mass and 5% by mass or less.

[0010] Elastin may be hydrolyzed. This composition may further contain brown algae extract in a concentration greater than 0% by mass and less than or equal to 5% by mass. The brown algae extract may be Saccharina longicluris extract. This composition may further contain glycerin, Saccharina longicluris extract, hydrolyzed elastin, sodium hyaluronate, acrylates / C10-30 alkyl acrylate crosspolymer, benzyl alcohol, ethylhexylglycerin, tocopherol, (caprylic / capric acid) coconut alkyl, sodium gluconate, and sodium hydroxide, or a combination thereof. This composition may contain 91.8% by mass of oxygenated water, 0.70% by mass of acrylates / C10-30 alkyl acrylate crosspolymer, 0.75% by mass of sodium hydroxide, 1.00% by mass of benzyl alcohol, ethylhexylglycerin, and tocopherol, 1.50% by mass of glycerin, water, and Laminaria saccharina extract, 0.20% by mass of sodium gluconate, 2.00% by mass of glycerin, 1.00% by mass of hydrolyzed elastin, 1.00% by mass of sodium hyaluronate, and 0.05% by mass of (caprylic / capric acid) coconut alkyl.

[0011] The composition may be in a form selected from gel, cream, liquid, spray, and mask. The composition may be in the form of a cream and may contain oxygenated water at a concentration of 20-25% by mass.

[0012] Another aspect of the present invention provides the use of any of the above compositions for regenerating deep skin tissue. This use includes applying the composition to the skin tissue to be treated. This use may further include applying the composition to the skin tissue after ablative laser, non-ablative laser, Coupereau laser, and rosacea treatment. This use may further include applying the composition to the skin tissue to be treated once a day for four consecutive days, and twice a day for five consecutive days.

[0013] The features considered novel in this invention are described in detail in the appended claims. [Brief explanation of the drawing]

[0014] The above-mentioned and other objectives, features, and advantages of the present invention will become more readily apparent by referring to the following description and accompanying drawings.

[0015] [Figure 1] Figure 1 shows the strength of collagen type I, comparing the effects of using a collagen-containing composition based on the principle of the present invention with the effects of using a placebo composition. [Figure 2] Figure 2 shows the strength of collagen type III, comparing the effects of using a collagen-containing composition based on the principle of the present invention with the effects of using a placebo composition. [Figure 3] Figure 3 is a graph comparing the intensity of hyaluronic acid present in skin tissue after use of a collagen-containing composition based on the principle of the present invention and after use of a placebo composition. [Figure 4] Figure 4 shows mature skin tissue in which collagen and elastin are damaged and / or deficient. [Figure 5] Figure 5 shows how the composition based on the principle of the present invention is used on the mature skin tissue shown in Figure 4. [Figure 6] Figure 6 shows the effects of using this composition on the mature skin tissue shown in Figure 5. [Figure 7] Figure 7 shows the effects of using a collagen-containing composition based on the principle of the present invention. [Figure 8] Figure 8 shows the effects of using a collagen-containing composition based on the principle of the present invention. [Figure 9] Figure 9 shows the effects of using a collagen-containing composition and an elastin-containing composition based on the principle of the present invention. [Figure 10] Figure 10 shows the effects of using a collagen-containing composition based on the principle of the present invention. [Modes for carrying out the invention]

[0016] The following describes novel compositions, methods, and uses for skin cell regeneration. While the present invention is described using specific exemplary embodiments, the embodiments described herein are merely illustrative and are not intended to limit the scope of the present invention.

[0017] Human skin generally functions as a protective barrier against external stimuli. For the skin to maintain its good barrier function, it must be supplied with nutrients necessary to maintain its health. More importantly, these nutrients must penetrate deep into the skin to maintain or rebuild the skin tissue layers, including the dermis, and to facilitate communication between these layers.

[0018] In some embodiments, the composition contains oxygenated water combined with other components. Oxygenated water generally helps the composition penetrate transdermally into the dermis of the user's skin. Therefore, oxygenated water enhances the transdermal penetration of the composition. To this end, the oxygenated water contains highly concentrated encapsulated oxygen nanobubbles. This results in a higher oxygen content in the oxygenated water than in the surrounding air, allowing the skin to absorb the oxygenated water better. For example, the composition may contain more than 50 mg / L of dissolved oxygen in addition to 42 million encapsulated oxygen nanobubbles. Transdermal penetration of oxygenated water generally aims to promote keratinocyte epithelialization, increase the angiogenesis process, stimulate connective tissue formation and / or increase the amount of collagen in the skin, and further stimulate a local immune response to promote skin healing. Transdermal penetration of compositions containing oxygenated water contributes to the regeneration of the dermis and epidermis.

[0019] In one embodiment, the composition may further contain collagen configured to penetrate the skin of the user. Collagen is the most abundant protein in the skin and may account for up to 70% of the skin's composition. Collagen present in the skin absorbs moisture and plays roles such as providing elasticity, cell regeneration, and reducing wrinkles. Therefore, the collagen contained in this composition generally aims to increase the amount of collagen in the skin, such as type I and type III collagen. Collagen can further stimulate hyaluronic acid and provide essential amino acids for skin tissue. Essential amino acids may have a low molecular weight such as 3 - 6 Da.

[0020] The composition may contain one or more types of collagen. As an example, the composition may contain marine collagen, and marine collagen includes, but is not limited to, marine collagen powder, hydrolyzed collagen, hydrolyzed marine collagen, or combinations thereof. Naturally, other types of collagen from various raw materials and in various forms can also be used. A composition containing hydrolyzed collagen can improve the interaction between collagen and skin tissue, whether it is of marine origin or not. Hydrolyzed collagen may have a low molecular weight and may contain free amino acids such as glycine, proline, hydroxyproline, etc. As an example, hydrolyzed collagen has a molecular weight (Mw) of 3 - 6 KDa, an isoelectric point (pI) of 3.68 - 5.7, and low viscosity and does not show film-forming properties. Therefore, hydrolyzed collagen is significantly different from natural collagen, which has a molecular weight (Mw) of 300 KDa, an isoelectric point (pI) of 7.0 - 8.3, and high viscosity and shows film-forming properties.

[0021] Figures 1 to 3 show the effects of the use of a composition containing hydrolyzed collagen based on the principles of the present invention. In the tests, hydrolyzed marine collagen is used, but similar results can be obtained with other types of collagen. During the tests, the composition containing collagen was applied twice a day for 72 hours. Figures 1 and 2 show the results after using the placebo composition on the left side and the results after using the composition containing collagen on the right side. Figure 1 shows the presence of type I collagen, and Figure 2 shows the presence of type II collagen. Since the color tone intensity of the test results of the composition containing collagen is higher compared to the test results of the placebo, it can be observed that the amounts of type I and type III collagen have increased significantly. Figure 3 shows the intensity of hyaluronic acid in the skin tissue treated with the composition containing collagen compared to the placebo composition. It can be observed that the composition containing collagen increased the hyaluronic acid intensity by 25%. Therefore, the tests conducted clearly show that the presence of hydrolyzed collagen such as hydrolyzed marine collagen improves the intensity of type I and type III collagen and hyaluronic acid in the skin.

[0022] This composition may further contain additional ingredients. The additional ingredients may include any one of propanediol, silanetriol, organosilicon, hyaluronic acid, or a combination thereof. The purpose of the additional ingredients is to improve the communication with the extracellular matrix, promote the synthesis of type I and type III collagen, promote the synthesis of hyaluronic acid, activate fibroblasts, and supply nutrients to the dermis. The presence of stem cells in a composition containing organosilicon, etc. is generally understood to reduce the impact of stress on the skin, usually in combination with hyaluronic acid, and it may further improve skin regeneration.

[0023] In one embodiment of the present invention, the composition may contain any one of the following, or a combination thereof: oxygenated water, propanediol, silanetriol, hyaluronic acid, hydrogenated collagen, soluble collagen peptide, acrylates / C10-30 alkyl acrylate crosspolymer, benzyl alcohol, ethylhexylglycerin, tocopherol, (caprylic / capric acid) coconut alkyl, sodium gluconate, and sodium hydroxide.

[0024] In another embodiment of the present invention, an exemplary composition contains the following components at the following concentrations. [Table 1]

[0025] In yet another embodiment, the collagen-containing composition described above can be applied as a gel, liquid, topical cream, or other type of application medium.

[0026] In further embodiments, the composition may contain the above components in a wider range of values. For example, the oxygenated water may be at a concentration of 80-95% by mass. In such embodiments, the composition may be a gel or the like. In another example, the oxygenated water may be at a concentration of 20-30% by mass. In such embodiments, the composition may be a cream. It is understood that other types of collagen can be further added to the composition by changing the weight values ​​of the components in the composition, i.e., by changing the weight value of the oxygenated water. In some examples, the weight of marine collagen may vary in the range of greater than 0% by mass and less than or equal to 5% by mass. Naturally, the composition containing silanetriol, organosilicon, and hyaluronic acid may further contain them in the range of greater than 0% by mass and less than or equal to 5% by mass.

[0027] In other embodiments, the composition may contain elastin. Elastin is a type of structural protein family. Therefore, elastin can be used to maintain the density level, hardness, and elasticity of the skin. The elastin in the composition generally plays a role in regenerating the extracellular matrix in the deeper layers of the skin. Elastin can be used to increase the density of elastin fibers in the skin, improve skin tone, and reconstruct the skin to improve density and firmness.

[0028] In some embodiments, the composition may further contain brown algae such as Saccharina longiculis, also known as Laminaria saccharina. Algae can improve the elasticity and density of elastin fibers in the skin, increase the concentration of hyaluronic acid in the skin, and enhance the protective barrier function of the skin.

[0029] In yet another embodiment of the present invention, an exemplary composition containing elastin contains the following components. [Table 2]

[0030] In some embodiments, the composition may contain one or a combination thereof of oxygenated water, glycerin, Laminaria saccharina extract, hydrolyzed elastin, sodium hyaluronate, acrylates / C10-30 alkyl acrylate crosspolymer, benzyl alcohol, ethylhexylglycerin, tocopherol, (caprylic / capric acid) coconut alkyl, sodium gluconate, and sodium hydroxide.

[0031] In further embodiments, the composition may contain the above components in a wider range of values. For example, oxygenated water may be contained at a concentration of 80-95% by mass. In such embodiments, the composition may be a gel or the like. In another example, oxygenated water may be contained at a concentration of 20-30% by mass. In such embodiments, the composition may be a cream.

[0032] A composition containing elastin may further contain other types of elastin or collagen, such as marine collagen, in a concentration of more than 0% by mass and up to 5% by mass. Naturally, a composition containing glycerin, water, and Laminaria saccharina extract may further contain them in a range of more than 0% by mass and up to 5% by mass.

[0033] This composition may be contained in gels, creams, liquids, sprays, masks, or any other application medium known in the art of topical skin treatment. In compositions contained in sprays, etc., this composition may contain both hydrolyzed elastin and hydrolyzed collagen. Hydrolyzed elastin and hydrolyzed collagen may be contained in the composition in any of the above-mentioned ratios, and the ratio of oxygenated water is adjusted accordingly.

[0034] The use of skin cell regeneration compositions is also disclosed. In some embodiments, the use involves applying an embodiment of the skin cell regeneration composition described above to a skin area to be treated once daily. The use may further include using the embodiment of the skin cell regeneration composition until the appearance of the treated skin stabilizes or improves.

[0035] This composition may be used for the treatment of scars, such as postoperative scars. This composition may also be used on skin after treatments such as ablative lasers, high-intensity pulsed light (IPL), non-ablative lasers such as neodymium-doped yttrium aluminum garnet (ND:YAG) lasers, Coupereau lasers, and rosacea treatment. In yet another embodiment, this use may involve applying the composition to a treated or surgically altered skin area immediately after a medical procedure or surgery. This method further includes applying the composition to the treated or surgically altered skin area at least once a day for the first 0-4 days, and at least twice a day thereafter, until the skin is completely healed. Naturally, this composition may also be used on skin damaged for reasons not exemplified above.

[0036] Figures 4-6 show the effects of using the above-mentioned collagen and elastin-containing composition. Figure 4 shows mature skin tissue with damaged collagen and elastin. Figure 5 shows the deep penetration when this composition is used on mature skin tissue. Figure 6 shows the effect of using this composition on mature skin tissue. It can be seen that the damaged skin tissue shown in Figure 1 is substantially repaired.

[0037] Figure 7 illustrates the exemplary effects of using an embodiment of a collagen-containing composition based on the principle of the present invention. In this example, the user used the composition twice a day for 30 days. The left side of Figure 7 shows the user's skin appearance before using the composition. As shown, the user has noticeable wrinkles in at least three specific areas. The right side of Figure 7 shows the user's skin appearance after using the composition for 30 consecutive days. The same areas as on the left side are shown, and it can be observed that the appearance of wrinkles has been reduced.

[0038] Figure 8 illustrates the exemplary effects of using an embodiment of a collagen-containing composition based on the principle of the present invention. In this example, an embodiment of the composition was applied to the user's skin twice a day for 60 consecutive days. The left side of Figure 8 shows the appearance of the user's skin before using the composition. As shown in the figure, the user's skin has stretch marks due to childbirth. The right side of Figure 8 shows the appearance of the user's skin after using the composition. As shown in the figure, the appearance of the stretch marks that were present before use has improved. That is, the size and / or number of stretch marks have been reduced.

[0039] Figure 9 shows exemplary effects obtained by using embodiments of two compositions based on the principle of the present invention. The first composition contains collagen, and the second composition contains elastin. The first and second compositions were applied to the user's skin twice a day for 28 consecutive days. Comparing the left and right figures showing the skin before and after use of the composition, it can be observed that the skin tissue around the neck of the user to whom the composition was applied is significantly firmer and denser.

[0040] Figure 10 illustrates the exemplary effects of using an embodiment of a collagen-containing composition based on the principle of the present invention. The composition was applied to the user's skin twice a day for 14 consecutive days. The left side of Figure 10 shows the appearance of the user's skin before using the composition. As shown in the figure, the user suffered a skin injury. The skin injury is a clear cut. The right side of Figure 10 shows the appearance of the user's skin after applying the composition to the injured area. As shown in the figure, the skin cut is observed to have scarred and is almost invisible.

[0041] While exemplary and currently preferred embodiments of the present invention have been described in detail, the concepts of the present invention can be embodied and applied in a variety of other forms, and the appended claims also include variations thereof, unless limited by the prior art.

Claims

1. A composition for regenerating deep skin cells, 80-95% by mass of oxygenated water, Collagen is greater than 0% by mass and less than or equal to 5% by mass. A composition containing the following concentration.

2. The composition according to claim 1, wherein the collagen is one of marine collagen powder, hydrolyzed collagen, and hydrolyzed marine collagen, or a combination thereof.

3. Furthermore, the composition according to claim 1, further containing organosilicon in a concentration exceeding 0% by mass and not exceeding 5% by mass.

4. The composition according to claim 3, wherein the organosilicon is silanetriol.

5. The composition according to claim 1, further comprising propanediol, hyaluronic acid, organosilicon, soluble collagen peptide, acrylates / C10-30 alkyl acrylate crosspolymer, benzyl alcohol, ethylhexylglycerin, tocopherol, (caprylic / capric acid) coconut alkyl, sodium gluconate, and sodium hydroxide, or a combination thereof.

6. 90.8% by mass of oxygenated water, Acrylates / C10-30 alkyl acrylate crosspolymer, 0.70% by mass, 0.75% by mass of sodium hydroxide, Benzyl alcohol, ethylhexylglycerin, and tocopherol are present in a concentration of 1.00% by mass. Organosilicon and hyaluronic acid in an amount of 1.50% by mass, 0.20% by mass of sodium gluconate, 2.00% by mass of propanediol, Sodium hyaluronate, 2.00% by mass, Water and hydrolyzed collagen in a quantity of 1.00% by mass, (Caprylic / Capric Acid) coconut alkyl 0.05% by mass, The composition according to claim 5, containing at the concentration of [amount].

7. Furthermore, the composition according to claim 1, further containing elastin in a concentration greater than 0% by mass and less than or equal to 5% by mass.

8. The composition according to claim 1, wherein the composition is in a form selected from a gel, cream, liquid, spray, and mask.

9. The composition according to claim 1, wherein the composition is in the form of a cream and contains oxygenated water at a concentration of 20 to 25% by mass.

10. A composition for regenerating deep skin cells, 80-95% by volume of oxygenated water, Elastin in an amount greater than 0% by mass and less than or equal to 5% by mass, A composition containing the following concentration.

11. The composition according to claim 10, wherein the elastin is hydrolyzed.

12. Furthermore, the composition according to claim 10, further containing a brown algae extract in a concentration greater than 0% by mass and less than or equal to 5% by mass.

13. The composition according to claim 12, wherein the brown algae extract is Saccharina longiculis extract.

14. The composition according to claim 10, further comprising glycerin, Saccharina longiculis extract, hydrolyzed elastin, sodium hyaluronate, acrylates / C10-30 alkyl acrylate crosspolymer, benzyl alcohol, ethylhexylglycerin, tocopherol, (caprylic / capric acid) coconut alkyl, sodium gluconate, and sodium hydroxide, or a combination thereof.

15. Oxygenated water at 91.8% by mass, Acrylates / C10-30 alkyl acrylate crosspolymer, 0.70% by mass, 0.75% by mass of sodium hydroxide, Benzyl alcohol, ethylhexylglycerin, and tocopherol are present in a concentration of 1.00% by mass. Glycerin, water, and Laminaria saccharina extract in 1.50% by mass, 0.20% by mass of sodium gluconate, Glycerin at 2.00% by mass, Hydrolyzed elastin, 1.00% by mass, Sodium hyaluronate is 1.00% by mass, and (Caprylic / Capric Acid) coconut alkyl 0.05% by mass, The composition according to claim 14, containing at the concentration of [amount].

16. The composition according to claim 10, which is in a form selected from a gel, cream, liquid, spray, and mask.

17. The composition according to claim 10, wherein the composition is in the form of a cream and contains oxygenated water at a concentration of 20 to 25% by mass.

18. The use of the composition for regenerating deep skin tissue according to claim 1, comprising applying the composition to skin tissue to be treated.

19. Furthermore, the use according to claim 18 includes applying the composition to the skin tissue after ablative laser, non-ablative laser, Coupereau laser and rosacea treatment.

20. The use according to claim 18, comprising applying the composition to the skin tissue to be treated once a day for four consecutive days, and twice a day for five consecutive days.

21. The use of the composition for regenerating deep skin tissue according to claim 10, comprising applying the composition to skin tissue to be treated.

22. Furthermore, the use according to claim 21 comprises applying the composition to the skin tissue after ablative laser, non-ablative laser, Coupereau laser and rosacea treatment.

23. The use according to claim 22, comprising applying the composition to the skin tissue to be treated once a day for four consecutive days, and twice a day for five consecutive days.

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