NAD(P)H oxidase

A stable NADH and NADPH oxidase from Carnobacterium divergens addresses the limitations of existing enzymes by maintaining activity in organic solvents and facilitating efficient cofactor regeneration in industrial processes.

JP2026523098APending Publication Date: 2026-07-10ANNIKKI GMBH
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
ANNIKKI GMBH
Filing Date
2024-07-08
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing NAD(P)H oxidases exhibit low stability and turnover number under oxidative process conditions, particularly in the presence of organic solvents, and require additional substrates, complicating industrial biocatalytic processes.

Method used

A novel NADH and NADPH oxidase derived from Carnobacterium divergens, with specific amino acid sequences, demonstrates high stability and activity in the presence of organic solvents and does not require additional substrates, suitable for cofactor regeneration in industrial processes.

Benefits of technology

The enzyme maintains high stability and activity in organic solvents, enabling efficient cofactor regeneration in one-pot reactions and cascade reactions, reducing the need for additional stabilizing additives and enhancing yield.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure 2026523098000001_ABST
    Figure 2026523098000001_ABST
Patent Text Reader

Abstract

The present invention includes a step of incubating a protein having NADH and / or NADPH oxidase activity together with NADH and / or NADPH in an aqueous solution, wherein NADH is converted to NAD + to and / or NADPH to NADP + A method for oxidation, wherein the protein comprises an amino acid sequence selected from the group consisting of the following, relating to the method i) An amino acid sequence having at least 80% sequence identity with Sequence ID No. 1, ii) an amino acid sequence encoded by a nucleic acid sequence having at least 80% identity with SEQ ID NO: 2, and iii) an amino acid sequence encoded by a nucleic acid that binds to a nucleic acid molecule complementary to the nucleic acid sequence of SEQ ID NO: 2 under stringent conditions, wherein stringent conditions preferably include washing at 65°C and with a salt concentration of 0.1 to 2 × SSC.
Need to check novelty before this filing date? Find Prior Art