A three-mode phytic acid detection method based on a double-enzyme cascade reaction and application thereof

By employing a dual-enzyme cascade reaction combining the CAU-10-NH2 metal-organic framework with ALP enzymatic reaction, a three-modal detection of phytic acid was achieved. This solves the problems of high cost, cumbersome process, and susceptibility to environmental interference in existing detection methods, improves the selectivity and sensitivity of the detection, and is suitable for rapid quantitative analysis of food and environmental water samples.

CN122409545APending Publication Date: 2026-07-17QUFU NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QUFU NORMAL UNIV
Filing Date
2026-04-24
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing methods for detecting phytic acid suffer from problems such as expensive instruments, cumbersome operation, insufficient sensitivity and selectivity, and susceptibility to environmental interference due to the single detection mode, making it difficult to cope with the various interference factors in complex food and environmental water samples.

Method used

A three-modal detection method based on a dual-enzyme cascade reaction was adopted, using CAU-10-NH2 metal-organic framework as a photoresponsive nanozyme, combined with ALP enzymatic reaction, to detect phytic acid through three modes: fluorescence, UV-Vis absorption spectroscopy and photothermal imaging, integrating fluorescence, colorimetric and photothermal signal output.

Benefits of technology

It improves the selectivity and sensitivity of detection, effectively avoids the influence of common interfering substances, is easy to operate, and is suitable for rapid quantitative analysis of food and environmental water samples, expanding its applicability in the detection of complex matrices.

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Abstract

The application belongs to the technical field of nano-enzyme sensing, and particularly relates to a three-mode phytic acid detection method based on a double-enzyme cascade reaction and application thereof. The detection method uses CAU-10-NH2 metal organic framework as a light-responsive nano-enzyme, combines the light catalytic oxidase activity of ALP and CAU-10-NH2, acquires signals through three modes of fluorescence spectrum, ultraviolet-visible absorption spectrum and photothermal imaging, and quantitatively analyzes the phytic acid content according to the signal change. Phytic acid inhibits the ALP activity, controls the generation amount of ascorbic acid, further influences the oxTMB content generated by CAU-10-NH2 catalyzing TMB oxidation, and realizes the synchronous change of fluorescence, colorimetric and photothermal signals. The application first discovers that CAU-10-NH2 has light catalytic oxidase activity, and only the -NH2 substituted derivative in the CAU-10 series (including CAU-10-H, -OH, -Br, -NO2, etc.) exhibits the activity, which expands the application of aluminum-based MOFs in the field of nano-enzyme sensing.
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