Collagen absorption enhancer

Abstract

To provide a collagen absorption accelerating agent which makes collagen be efficiently absorbed into a body and a method of accelerating absorption of collagen.SOLUTION: A collagen absorption accelerating agent includes at least one or more maltobionic acids selected from the group consisting of maltobionic acid expressed by a general formula of 4-O-α-D-glucopyranosyl-D-gluconate and salt thereof. The maltobionic acid salt may include one of maltobionic acid sodium, maltobionic acid calcium, maltobionic acid magnesium, maltobionic acid potassium, maltobionic acid copper, maltobionic acid iron, and maltobionic acid zinc.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a collagen absorption enhancer and a method for enhancing collagen absorption. [Background technology]

[0002] Collagen is an animal protein that accounts for approximately 30% of biological protein, and is abundant as the main component of the cell matrix that constitutes tendons, ligaments, joints, bones, blood vessels, skin, etc. Collagen plays important roles such as supporting cells and skin tissue, retaining water in intercellular spaces, and maintaining the lubricity and flexibility of skin. In bones, collagen accounts for the majority of the components in bone tissue, and is said to be an important component in maintaining bone quality.

[0003] However, collagen, which plays an important role in the body, decreases with the decline in metabolism due to aging, and it is known that in skin tissue, this can lead to dry skin, rough skin, and reduced elasticity and flexibility, and in bone tissue, this can lead to reduced bone strength due to osteoporosis and increased risk of arthritis. Therefore, in order to maintain healthy skin and bone functions, it is desirable to maintain or efficiently replenish insufficient collagen.

[0004] From these viewpoints, daily intake of foods containing collagen is recommended, and ingestion of collagen hydrolyzed peptides, collagen decomposition inhibitors in vivo, collagen synthesis promoters, etc. have been proposed (see Patent Documents 1 to 3). [Prior art documents] [Patent documents]

[0005] [Patent Document 1] JP 2004-244369 A [Patent Document 2] JP 2011-256136 A [Patent Document 3] JP 2016-056116 A Summary of the Invention [Problem to be solved by the invention]

[0006] However, collagens such as collagen and collagen hydrolyzed peptides are difficult to be absorbed in the body, so in order to exert the physiological effects of collagens in the body, it is necessary to ingest a large amount of collagens.In addition, the bioavailability of collagens is not good.In view of this situation, it is desirable to be able to more efficiently absorb ingested collagens in the body.

[0007] The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a collagen absorption promoter that can efficiently absorb collagens into the body, and a method for promoting collagen absorption. [Means for solving the problem]

[0008] The present inventors have surprisingly found that maltobionic acid and maltobionate salts promote the absorption of collagen into the body, and have thus completed the present invention. Specifically, the present invention provides the following.

[0009] (1) A collagen absorption promoter comprising at least one maltobionic acid selected from the group consisting of maltobionic acid represented by the general formula 4-O-α-D-glucopyranosyl-D-gluconic acid and its salts.

[0010] (2) The collagen absorption promoter according to (1), wherein the salt is at least one selected from calcium maltobionate, magnesium maltobionate, sodium maltobionate, potassium maltobionate, copper maltobionate, iron maltobionate and zinc maltobionate.

[0011] (3) The collagen absorption enhancer according to (1) or (2), which is a food or drink.

[0012] (4) The collagen absorption enhancer according to any one of (1) to (3), which is orally administered simultaneously with or successively to a collagen.

[0013] (5) A method for promoting collagen absorption, comprising blending a collagen absorption promoter according to any one of (1) to (4) to collagen. Effect of the Invention

[0014] According to the present invention, it is possible to provide a collagen absorption enhancer that can efficiently absorb collagen into the body and a method for promoting collagen absorption. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0015] <Collagen absorption enhancer> The collagen absorption enhancer of the present invention contains at least one maltobionic acid selected from the group consisting of maltobionic acid represented by the general formula 4-O-α-D-glucopyranosyl-D-gluconic acid and salts thereof.

[0016] The collagen absorption promoter of the present invention contains maltobionic acids, and therefore can promote the absorption of collagens such as collagen and collagen hydrolyzed peptides, and can increase the amount of collagen absorbed into the body, as shown in the examples described below. This allows collagens to be efficiently absorbed into the body. The collagen absorption promoter of the present invention can sustain the retention of collagens in the body. For example, the collagens can be maintained in a high concentration in the body even after 4 hours or more have passed since the collagens and collagen absorption promoter were ingested. Therefore, by ingesting the collagen absorption enhancer of the present invention, it is possible to prevent or improve dry skin, rough skin, loss of elasticity and flexibility, osteoporosis, osteoarthritis, and the like.

[0017] The maltobionic acid contained in the collagen absorption enhancer is maltobionic acid represented by the general formula 4-O-α-D-glucopyranosyl-D-gluconic acid or its salts. The collagen absorption enhancer may contain both maltobionic acid and its salts. Examples of the salt form include calcium salt, magnesium salt, sodium salt, iron salt, potassium salt, zinc salt, and copper salt. There is no limitation on the form of the maltobionic acids contained in the collagen absorption enhancer, and it may be in liquid or powder form.

[0018] The content of maltobionic acids can be appropriately set depending on the type and form of the collagen absorption enhancer, and is, for example, 0.0001% by mass to 100% by mass, preferably 0.001% by mass to 50% by mass, more preferably 0.01% by mass to 30% by mass, and even more preferably 0.01% by mass to 10% by mass relative to the collagen absorption enhancer. Since maltobionic acids exert the effect of promoting the absorption of collagen even in small amounts, the content of maltobionic acids may be 1.0% by mass or less relative to the collagen absorption enhancer.

[0019] The collagen absorption enhancer of the present invention may contain only maltobionic acids, or may contain maltobionic acids and other components. Examples of other components include functional components, nutritional supplements, sugars, sweeteners, acidulants, colorants, thickeners, glossing agents, and manufacturing agents. In addition to these, various excipients, binders, lubricants, stabilizers, diluents such as water, bulking agents, thickeners, emulsifiers, colorants, flavorings, additives, and seasonings that are conventionally contained in foods and beverages can be used as other components. The content of other components can be appropriately selected depending on the form of the collagen absorption promoter of the present invention. In addition, when the collagen absorption promoter further contains other collagen absorption promoters different from maltobionic acids, the content of maltobionic acids may be reduced.

[0020] The form of the collagen absorption promoter is not particularly limited, but can be, for example, an oral collagen absorption promoter. The form of the oral collagen absorption promoter is not particularly limited, and can be any form. For example, forms suitable for oral intake, specifically, liquid, powder, granule, tablet, rod, plate, block, solid, round, candy, paste, cream, capsule such as hard capsule or soft capsule, caplet, tablet, gel, jelly, gummy, wafer, biscuit, cookie, chewable, syrup, stick, etc. can be mentioned. For example, in the case of a solid collagen absorption promoter, liquid maltobionic acid can be powdered together with an excipient to form a solid.

[0021] The method of using the collagen absorption enhancer is not particularly limited. For example, in the case of a liquid collagen absorption enhancer, it can be orally ingested by drinking it as is or after diluting it with water or the like.

[0022] The collagen absorption enhancer is used, for example, by orally administering it simultaneously with or successively to the collagen. The subjects for oral administration are, for example, mammals such as humans. When the collagen absorption promoter is orally administered simultaneously with collagens, the collagen absorption promoter may contain collagens, or the collagen absorption promoter and collagens as a separate agent from the collagen absorption promoter may be orally administered simultaneously. In addition, when the collagen absorption enhancer and collagens are orally administered consecutively, either the collagen absorption enhancer or the collagens may be orally administered first, or either one of the collagen absorption enhancer and the collagens may be orally administered and, after a predetermined period of time, the other may be orally administered.

[0023] The intake amount of the collagen absorption promoter is not particularly limited and can be set appropriately depending on the condition (e.g., degree of bone density) and intake manner of the recipient (e.g., mammal such as human), but the daily intake amount of the collagen absorption promoter for adults is, for example, 5 to 400 mg / kg, preferably 10 to 200 mg / kg, and more preferably 20 to 100 mg / kg, based on the recipient's body weight, converted into the mass of maltobionic acids.

[0024] The mass ratio of the intake of maltobionic acids to the intake of collagens (the intake mass of maltobionic acids / the intake mass of collagens) is, for example, 0.03 to 24.00, preferably 0.06 to 12.00, and more preferably 0.12 to 6.00. Since even a small amount of maltobionic acids exhibits the effect of promoting the absorption of collagens, the mass ratio of the intake of maltobionic acids to the intake of collagens may be 1.0 or less, or even 0.5 or less.

[0025] The collagen absorption enhancer of the present invention may be a food or drink (including health-conscious food or drink, for example, nutritional supplements, health supplements, nutritionally adjusted foods, health functional foods, foods for specified health uses, nutritionally functional foods, foods with functional claims, etc.), feed, or a pharmaceutical composition (drugs, quasi-drugs, etc.). That is, the collagen absorption enhancer of the present invention can be used as an oral composition such as a food or drink, feed, or pharmaceutical composition.

[0026] <Methods to promote collagen absorption> The method for promoting collagen absorption of the present invention includes blending the above-mentioned collagen absorption promoter with collagen. Since the above-mentioned collagen absorption promoter contains maltobionic acids, blending (adding) the collagen absorption promoter with collagen can promote the absorption of collagen into the body when the collagen is ingested. EXAMPLES

[0027] The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples.

[0028] <Example 1 and Comparative Example 1> (1) Verification of the collagen absorption-promoting effect of maltobionic acids The collagen absorption-promoting effect of maltobionic acid was verified by measuring blood hydroxyproline concentrations over time after collagen intake. Hydroxyproline accounts for approximately 11-14% of the amino acids that make up collagen, and is a substance that is used as an indicator of collagen absorption because it is characteristically present in collagen.

[0029] (2) Preparation of test samples Test foods of Comparative Example 1 (placebo sample) and Example 1 (test sample) were prepared by mixing mineral water (Suntory Foods Limited), collagen peptide (SCP-30NB, Nitta Gelatin Co., Ltd.), maltose syrup (Sanei Saccharification Co., Ltd.), and maltobionic acid (Sanei Saccharification Co., Ltd.) in the formulation (amount (g)) shown in Table 1.

[0030] [Table 1]

[0031] (3) Maltobionic acid intake test Eleven healthy adult males and females (aged 22 to 51 years) were used as subjects. From 10 p.m. on the day before the test until the end of the test, subjects were prohibited from eating or drinking anything other than water and the test food provided on the day of the test. On the day of the test, blood was collected, and the subjects were allowed to consume the test food without being informed whether it was a placebo sample or a test sample. Blood was collected 2 hours, 4 hours, and 6 hours after ingestion. The test was a crossover single-dose blinded test, and subjects who had consumed the placebo sample first were allowed to consume the test sample, and subjects who had consumed the test sample first were allowed to consume the placebo sample, and the test was conducted in the same manner.

[0032] (4) Evaluation items and evaluation methods The blood was left at room temperature for 30 minutes or more, then centrifuged at 3000 rpm for 5 minutes, the supernatant was collected, and serum was obtained. The amount of hydroxyproline (nmol / mL) in the serum was measured using a kit (Hydroxyproline Assay Kit, CBL).

[0033] (5) Statistical processing method All results were expressed as mean ± standard error, and the significance of differences between two groups was determined by Student's t-test at a risk level of 5%.

[0034] (6) Changes in serum hydroxyproline concentration The time course of serum hydroxyproline concentration after ingestion of the test food (test sample or placebo sample) is shown in Table 2. Compared with the placebo sample (Comparative Example 1), a significant increase in serum hydroxyproline concentration was confirmed in the test sample (Example 1) 2 and 4 hours after ingestion of the test food.

[0035] [Table 2]

[0036] (7) Serum hydroxyproline time curve area Furthermore, the area under the curve (AUC) of serum hydroxyproline over time was determined for each subject. Specifically, the change in blood hydroxyproline was determined by subtracting the measured value before ingestion of the test food from the measured value at each time obtained in the above (6) Change in serum hydroxyproline concentration over time, and this change was plotted against the time elapsed since ingestion of the test food, and the area under the resulting broken line was determined as AUC. The obtained AUC values ​​are shown in Table 3. Compared with the placebo sample (Comparative Example 1), the test sample (Example 1) showed a significantly higher AUC for blood hydroxyproline amount.

[0037] [Table 3]

[0038] <Example 2 and Comparative Example 2> (1) Verification of the collagen absorption-promoting effect of maltobionic acids The collagen absorption promoting effect of calcium maltobionate was examined under the same test conditions as in Example 1.

[0039] (2) Preparation of test samples Test foods of Comparative Example 2 (placebo sample) and Example 2 (test sample) were prepared by mixing mineral water (Suntory Foods Limited), collagen peptide (SCP-30NB, Nitta Gelatin Co., Ltd.), maltose (Hayashibara Co., Ltd.), calcium carbonate (Sankyo Flour Milling Co., Ltd.), and calcium maltobionate (Sanei Sugar Chemical Co., Ltd.) in the formulation (amount (g)) shown in Table 4.

[0040] [Table 4]

[0041] (3) Calcium maltobionate intake test Twelve healthy adult males and females (aged 22 to 51 years) were used as subjects. From 10 p.m. on the day before the test until the end of the test, subjects were prohibited from eating or drinking anything other than water and the test food provided on the day of the test. On the day of the test, blood was collected, and the subjects were allowed to consume the test food without being informed whether it was a placebo sample or a test sample. Blood was collected 2 hours, 4 hours, and 6 hours after ingestion. The test was a crossover single-dose blinded test, and subjects who had consumed the placebo sample first were allowed to consume the test sample, and subjects who had consumed the test sample first were allowed to consume the placebo sample, and the test was conducted in the same manner.

[0042] (4) Evaluation items and evaluation methods The blood was left at room temperature for 30 minutes or more, then centrifuged at 3000 rpm for 5 minutes, the supernatant was collected, and serum was obtained. The amount of hydroxyproline (nmol / mL) in the serum was measured using a kit (Hydroxyproline Assay Kit, CBL).

[0043] (5) Statistical processing method All results were expressed as mean ± standard error, and the significance of differences between two groups was determined by Student's t-test at a risk level of 5%.

[0044] (6) Changes in serum hydroxyproline concentration The time course of the hydroxyproline concentration in serum after ingestion of the test food is shown in Table 5. Compared with Comparative Example 2, a significant increase in the hydroxyproline concentration in serum was confirmed in Example 2 2 and 4 hours after ingestion of the test food.

[0045] [Table 5]

[0046] (7) Serum hydroxyproline time curve area Furthermore, the area under the curve (AUC) of serum hydroxyproline over time was determined for each subject. The obtained AUC values ​​are shown in Table 6. Compared with Comparative Example 2, Example 2 showed a significantly higher AUC value for blood hydroxyproline amount.

[0047] [Table 6] [Industrial Applicability]

[0048] The collagen absorption enhancer of the present invention can enhance the absorption of collagens and increase the amount of collagen absorbed into the body, and is therefore extremely useful for maintaining beauty such as preventing dry or rough skin, and for maintaining the health of those who are concerned about bone strength.

Claims

1. A collagen absorption enhancer comprising at least one maltobionic acid selected from the group consisting of maltobionic acid represented by the general formula 4-O-α-D-glucopyranosyl-D-gluconic acid and salts thereof.

2. 2. The collagen absorption promoter according to claim 1, wherein the salt is at least one selected from sodium maltobionate, calcium maltobionate, magnesium maltobionate, potassium maltobionate, copper maltobionate, iron maltobionate and zinc maltobionate.

3. 3. The collagen absorption enhancer according to claim 1 or 2, which is a food or drink.

4. The collagen absorption enhancer according to any one of claims 1 to 3, which is orally administered simultaneously with or successively to collagen.

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