Antibodies to TIE-2 and methods of use

Abstract

The present invention relates to anti-Tie2 antibodies and methods of using them.

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of priority to U.S. Provisional Application No. 62 / 938,816, filed November 21, 2019, the disclosure of which is incorporated herein by reference in its entirety.

[0002] FIELD OF THEINVENTION The present invention relates to anti-Tie2 antibodies and methods of using them. [Background technology]

[0003] 2. Background of the Invention Tie2 is a receptor tyrosine kinase expressed primarily on the surface of endothelial cells and plays a central role in vascular stability, survival and maturation (Suri, C., et al., 1996. Requisite Role of Angiopoietin-1, a Ligand for the TIE2 Receptor, during Embryonic Angiogenesis. Cell 87(7):1171-80 (Non-Patent Document 1)); Thurston, G., et al., 1999. Leakage-Resistant Blood Vessels in Mice Transgenically Overexpressing Angiopoietin-1. Science 286(5449):2511-14 (Non-Patent Document 2); Saharinen, et al., 2010. How Do Angiopoietins Tie with Vascular Endothelial Growth Factors? Current Opinion in Hematology (Non-Patent Document 3); Augustin, et al., 2009. Control of Vascular Morphogenesis and Homeostasis through the Angiopoietin-Tie System. Nature Reviews. Molecular Cell Biology 10(3):165-77 (Non-Patent Document 4); Milam, et al., 2015. The Angiopoietin-Tie2 Signaling Axis in the Vascular Leakage of Systemic Inflammation. Tissue Barriers 3(1-2) (Non-Patent Document 5). Tie2 activity is tightly regulated by at least four soluble protein factors known as angiopoietins 1 to 4. Angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) are thought to be the main Tie2 function regulators. Under normal physiological conditions, high Ang1 and low Ang2 levels maintain constitutive activation of the Tie2 signaling axis.Specifically, Ang1 agonist ligands directly bind to the Tie2 receptor, leading to Tie2 clustering, autophosphorylation, and downstream signaling events, including activation of the PI3 kinase / Akt and MAPK pathways.

[0004] Gene targeting experiments have shown that the Ang / Tie signaling system is required for physiological and pathological remodeling of lymphatic and vascular vessels in fetal, postnatal and adult mice (Eklund L, Kangas J, Saharinen P. Angiopoietin-Tie signaling in the cardiovascular and lymphatic systems. Clin Sci (Lond) 2017; 131: 87-103 (Non-Patent Document 6)). In humans, altered expression of angiopoietin is involved in many vascular diseases (Saharinen P, Eklund L, Alitalo K. Therapeutic targeting of the angiopoietin-TIE pathway. Nat Rev Drug Discov 2017; 16: 635-61 (Non-Patent Document 7)).

[0005] There remains a need for anti-Tie2 antibodies with improved properties, and for their therapeutic and diagnostic uses. [Prior art documents] [Non-patent literature]

[0006] [Non-Patent Document 1] Suri, C., et al., 1996. Requisite Role of Angiopoietin-1, a Ligand for the TIE2 Receptor, during Embryonic Angiogenesis. Cell 87(7):1171-80 [Non-Patent Document 2] Thurston, G., et al., 1999. Leakage-Resistant Blood Vessels in Mice Transgenically Overexpressing Angiopoietin-1. Science 286(5449):2511-14. [Non-Patent Document 3] Saharinen, et al., 2010. How Do Angiopoietins Tie with Vascular Endothelial Growth Factors? Current Opinion in Hematology [Non-Patent Document 4] Augustin, et al., 2009. Control of Vascular Morphogenesis and Homeostasis through the Angiopoietin-Tie System. Nature Reviews. Molecular Cell Biology 10(3):165-77 [Non-Patent Document 5] Milam, et al., 2015. The Angiopoietin-Tie2 Signaling Axis in the Vascular Leakage of Systemic Inflammation. Tissue Barriers 3(1-2) [Non-Patent Document 6] Eklund L, Kangas J, Saharinen P. Angiopoietin-Tie signaling in the cardiovascular and lymphatic systems. Clin Sci(Lond)2017;131:87-103 [Non-Patent Document 7] Saharinen P, Eklund L, Alitalo K. Therapeutic targeting of the angiopoietin-TIE pathway. Nat Rev Drug Discov 2017;16:635-61 [Summary of the Invention]

[0007] The present invention provides anti-Tie2 antibodies and methods of using them for therapeutic and diagnostic purposes. The anti-Tie2 antibodies of the present invention exhibit unique properties that make them particularly suitable for use in therapy.

[0008] The following are specifically contemplated as part of the disclosed invention: Embodiment 1. An isolated anti-Tie2 antibody or antigen-binding fragment thereof, comprising three heavy chain complementarity determining regions (CDR H1-3) and three light chain CDRs (CDR L1-3) as follows: TIFF0007638989000001.tif109166TIFF0007638989000002.tif179166. Embodiment 2. An isolated anti-Tie2 antibody or antigen-binding fragment thereof, comprising: A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 242, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 243; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 244, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 245; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 246, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 247; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 248, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 249; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 250, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 251; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 252, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 253; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 254, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 255; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 256, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 257; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 258, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 259; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 260, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 261; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 262, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 263; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 264, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 265; A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 266, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 267; a heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 268 and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 269; or A heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO: 270, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO: 271. An isolated anti-Tie2 antibody or an antigen-binding fragment thereof, comprising: Embodiment 3. An isolated anti-Tie2 antibody, comprising: The antibody specifically binds to an epitope in the extracellular domain of human Tie2, The epitope is Amino acid residues K312, S316, C332, H358, K387, and T391 according to EU numbering such as Kabat numbering, as determined by cross-linking mass spectrometry 1. An isolated anti-Tie2 antibody comprising: Embodiment 4. The antibody of embodiments 1-3, wherein the antibody is an allosteric activator of Tie2. Embodiment 5. The antibody of embodiments 1-4, wherein the antibody is a non-ligand competitive binder of Tie2. Embodiment 6. The antibody of embodiments 3-5, wherein the antibody is cross-reactive with human, mouse, rat, rabbit and monkey Tie2. Embodiment 7. The antibody of any one of embodiments 1 to 6, wherein the antibody is fully human, humanized, monoclonal, or chimeric. Embodiment 8. The antibody of any one of embodiments 1 to 7, wherein the antibody is monospecific. Embodiment 9. The antibody of any one of embodiments 1 to 7, wherein the antibody is multispecific. Embodiment 10. The antibody of embodiment 9, wherein the multispecific antibody is bispecific. Embodiment 11. The bispecific antibody comprises: one binding arm that specifically binds human Tie-2 according to embodiment 8; a second binding arm that specifically binds VEGF-A, VEGF-B, VEGF-C, a VEGF variant, Ang-1, Ang-2, Ang-3, Ang-4, PDGF-β, interleukin-1β, VE-PTP, complement factor C3, integrin α5β1, amyloid beta, PD-1, PD-L1, or CTLA-4; 11. The antibody of embodiment 10, comprising: Embodiment 12 The antibody of embodiment 9, wherein the multispecific antibody is a biparatopic antibody. Embodiment 13. A biparatopic antibody comprising: one binding arm that specifically binds to a first epitope on the ECD of human Tie2; and the other binding arm specifically binds to a second epitope on the ECD of human Tie2. 13. The antibody of embodiment 12, comprising: Embodiment 14. The multispecific antibody is a trivalent, tetravalent, pentavalent, or hexavalent antibody, Trivalent, tetravalent, pentavalent, or hexavalent antibodies, At least one binding arm that specifically binds to human Tie2 according to embodiment 8; and other remaining binding arms that specifically bind VEGF-A, VEGF-B, VEGF-C, VEGF variants, Ang-1, Ang-2, Ang-3, Ang-4, PDGF-β, interleukin-1β, VE-PTP, complement factor C3, integrin α5β1, amyloid beta, PD-1, PD-L1, or CTLA-4. 10. The antibody of embodiment 9, comprising: Embodiment 15. The antibody according to any one of embodiments 1 to 14, wherein the antibody is an antibody fragment that specifically binds to human Tie2. Embodiment 16. The antibody of embodiment 15, wherein the antibody fragment is a Fab, Fab'-SH, Fv, scFv, or (Fab')2 fragment. Embodiment 17. The antibody of embodiment 16, wherein the multispecific antibody is composed of scFv antibody fragments linked together by a polypeptide linker. Embodiment 18. An antibody according to any one of embodiments 1 to 17, wherein the antibody possesses reduced effector function. Embodiment 19. The antibody of embodiment 18, wherein the antibody comprises at least one substitution mutation at amino acid residues N297, L234, L235, P329, D265, and E430 according to EU numbering, such as Kabat numbering. Embodiment 20. The antibody of embodiment 19, wherein at least one substitution mutation is selected from the group consisting of amino acid residues N297G, N297A, L234A, L235A, P329G, D265A, and E430G according to EU numbering, such as Kabat numbering. Embodiment 21 The antibody of embodiment 20, wherein the antibody comprises a substitution mutation at residue N297A or N297G. Embodiment 22 The antibody of embodiment 20, wherein the antibody comprises substitution mutations at residues L234A, L235A, and P329G. Embodiment 23 The antibody of embodiment 20, wherein the antibody comprises substitution mutations at residues D265A and N297G. Embodiment 24 The antibody of embodiment 21, wherein the antibody further comprises a substitution mutation at residue E430G. Embodiment 25 The antibody of embodiment 22, wherein the antibody further comprises a substitution mutation at residue E430G. Embodiment 26 The antibody of embodiment 23, wherein the antibody further comprises a substitution mutation at residue E430G. Embodiment 27 The antibody of embodiment 25, wherein the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 174, and a light chain comprising the amino acid sequence of SEQ ID NO: 175. 28. a heavy chain comprising the amino acid sequence of SEQ ID NO: 276, and a light chain comprising the amino acid sequence of SEQ ID NO: 277; a heavy chain comprising the amino acid sequence of SEQ ID NO: 278, and a light chain comprising the amino acid sequence of SEQ ID NO: 279; a heavy chain comprising the amino acid sequence of SEQ ID NO: 280, and a light chain comprising the amino acid sequence of SEQ ID NO: 281; a heavy chain comprising the amino acid sequence of SEQ ID NO: 282, and a light chain comprising the amino acid sequence of SEQ ID NO: 283; a heavy chain comprising the amino acid sequence of SEQ ID NO: 286 and a light chain comprising the amino acid sequence of SEQ ID NO: 287; or A heavy chain comprising the amino acid sequence of SEQ ID NO: 288, and a light chain comprising the amino acid sequence of SEQ ID NO: 289. 23. The antibody of embodiment 22, comprising: 29. the amino acid sequence of SEQ ID NO: 284, or Amino acid sequence of SEQ ID NO: 285 11. The antibody of embodiment 10, comprising: Embodiment 30. An isolated nucleic acid encoding an antibody according to any one of embodiments 1 to 29. Embodiment 31. A vector comprising the isolated nucleic acid of embodiment 30. Embodiment 32. A host cell comprising the vector of embodiment 31. Embodiment 33. A method for producing an antibody according to any one of embodiments 1 to 29, comprising: Culturing a host cell according to embodiment 32 in a culture medium; Isolating the resulting antibody. A method comprising: Embodiment 34. An immunoconjugate comprising an antibody according to any one of embodiments 1 to 29. Embodiment 35. A fusion polypeptide comprising an antibody according to any one of embodiments 1 to 29. Embodiment 36. A pharmaceutical composition comprising an antibody according to any one of embodiments 1 to 29, an immunoconjugate according to embodiment 34, or a fusion polypeptide according to embodiment 35. Embodiment 37. The pharmaceutical composition of embodiment 36, wherein the antibody, immunoconjugate, or fusion polypeptide is co-formulated with an anti-VEGF antibody or a VEGF extracellular trap protein. Embodiment 38. A method of treating a Tie2 dysregulated disease in a subject in need thereof, comprising administering to the subject a pharmaceutical composition according to embodiment 36. Embodiment 39. A method of treating a Tie2 dysregulated disease in a subject in need thereof, comprising administering to the subject a pharmaceutical composition according to embodiment 37. Embodiment 40 The method of embodiment 38, further comprising co-administering to the subject a pharmaceutical composition comprising an anti-VEGF antibody or a VEGF extracellular trap protein. Embodiment 41. The method of embodiments 38-40, wherein the Tie2 dysregulated disease comprises infectious disease, acute respiratory distress syndrome (ARDS), ischemic injury, eye damage, radiation injury, cancer, systemic sclerosis, traumatic brain injury, neuroinflammation, radiation injury, wound healing, myocardial infarction, blood-brain barrier damage, cerebral cavernous malformation, Duchenne muscular dystrophy (DMD) or Clarkson's disease. Embodiment 42 The method of embodiments 38-40, wherein the Tie2 dysregulated infection comprises sepsis, dengue virus infection, tuberculosis, or influenza. Embodiment 43. The method of embodiments 38-40, wherein the Tie2 dysregulated ischemic injury comprises diabetic nephropathy, acute kidney injury, chronic kidney disease, organ transplantation, critical limb ischemia, traumatic brain injury or stroke. Embodiment 44. The method of embodiments 38-40, wherein the Tie2 dysregulated ocular disorder comprises diabetic retinopathy, diabetic macular edema (DME), proliferative diabetic retinopathy (PDR) age-related macular degeneration (AMD), retinopathy of prematurity (ROP), or glaucoma. Embodiment 45. An isolated anti-Tie2 antibody according to embodiments 1 to 29, or an immunoconjugate according to embodiment 34, or a fusion polypeptide according to embodiment 35, for use in treating a Tie2 dysregulation disease according to embodiments 41 to 44. Embodiment 46. Use of an isolated anti-Tie2 antibody according to embodiments 1 to 29, or an immunoconjugate according to embodiment 34, or a fusion polypeptide according to embodiment 35, for the manufacture of a medicament for treating a Tie2 dysregulation disease according to embodiments 41 to 44. [Brief description of the drawings]

[0009] [Figure 1A] 1A-1C: Anti-Tie2 antibodies increase the levels of phospho-Tie2 (pTie2), as well as phospho-Akt (pAkt) and phospho-ERK (pERK), as determined by Western blotting. HUVEC cells were serum-starved and then treated with either PBS, Ang1 (12 nM), control hIgG1 (12 nM), or human anti-Tie2 antibodies #1, #2, and #3, or wild-type (WT) chicken anti-Tie2 antibody (12 nM) for 20 min, followed by cell lysis. FIG. 1A shows that Tie2 was immunoprecipitated from lysates using anti-Tie2 antibodies, and the levels of pTie2 were determined by Western blotting with an anti-phospho-tyrosine antibody. For pAkt and pERK analysis, Western blots of pAkt and total Akt (FIG. 1B), or pERK and total ERK (FIG. 1C), in whole cell lysates were performed. See Example 3. [Figure 1B] See legend to Figure 1A. [Figure 1C] See legend to Figure 1A. [Diagram 2]The functional potency of anti-Tie2 antibodies is comparable to Ang1 in vitro. HUVEC cells were serum-starved and treated with various concentrations (nM) of Ang1, human anti-Tie2 antibodies #1, #2 and #3, or WT chicken anti-Tie2 antibodies, followed by cell lysis. Western blots using pAkt-specific or total Akt-specific antibodies were performed. pAkt and total Akt signal intensity was measured using a LI-COR scanning fluorometer, and the level of pAkt normalized to total Akt (pAkt / Akt ratio) was plotted on the Y-axis using Graphpad™ Prism software, which was used to determine EC50 values. See Example 4. [Diagram 3] FIG. 1 shows a schematic diagram of a simplified in vitro model of the endothelial barrier, see Example 5. [Figure 4] Anti-Tie2 antibodies can reduce VEGF-induced cell permeability in an in vitro model of endothelial barrier leakage. Results are expressed as % of Ang1 activity (normalized to PBS-treated cells). For statistical analysis (using one-way ANOVA test), the effect of each antibody was compared to the effect of a non-specific IgG1 control antibody. Symbols: **P≦0.01, ****≦0.0001. See Example 5. [Figure 5A]Figure 5A-F: Anti-Tie2 antibody in combination with saturating amounts of Ang2 has additive effects on Tie2 downstream signaling in HUVEC cells. HUVEC cells were serum starved, treated with decreasing amounts of Ang2, lysed, and analyzed for levels of pERK and total ERK. Normalized values ​​are plotted on the Y-axis and concentrations of Ang2 are plotted on the X-axis (Figure 5A). Images were quantified using a LI-COR scanning fluorometer and pERK levels were normalized to total ERK. Serum starved HUVEC cells were treated with 43 nM Ang2 and 12 nM anti-Tie2 antibody for 20 min. Lysates were subjected to Western blotting and images were quantified for pERK and ERK (Figure 5C), and pAKT and AKT (Figure 5E), respectively. Levels of pERK (Figure 5D) and pAKT (Figure 5F) were normalized to total ERK and Akt levels, respectively, to adjust for equal protein loading. See Example 6. [Figure 5B] See legend to Figure 5A. [Figure 5C] See legend to Figure 5A. [Figure 5D] See legend to Figure 5A. [Figure 5E] See legend to Figure 5A. [Figure 5F] See legend to Figure 5A. [Figure 6] 6A and 6B: Anti-Tie2 antibodies do not compete with Ang1 (FIG. 6A) or Ang2 (FIG. 6B) for binding to the Tie2 extracellular domain (ECD). Representative competitive binding data using biolayer interferometry (BLI) are shown. The left panel shows loading of His-tagged Tie2 ECD protein (100 nM) onto a capture biosensor (Ni-NTA) with the start of exposure indicated by the arrow (1), followed by exposure to a solution containing individual anti-Tie2 antibodies (100 nM) as indicated by the arrow (2), followed by exposure to a solution containing Ang1 (FIG. 6A) or Ang2 ligand (FIG. 6B) (both at 100 nM) as indicated by the arrow (3). The y-axis indicates the magnitude of binding to the biosensor and the x-axis is time. See Example 7. [Figure 7]Anti-Tie2 antibodies show diversity of epitope recognition by BLI binding assessment. Recombinant human Tie2 ECD protein was loaded onto each biosensor and then exposed to a solution containing anti-Tie2 antibody (y-axis) followed by exposure to a second anti-Tie2 antibody (x-axis). The total wavelength shift (nm) after exposure to the second antibody is annotated in each box with a white number. Black boxes indicate a lack of binding of the second antibody (values ​​<0.2 nm). The lack of binding was only observed with the same antibody pair. This indicates that all antibodies tested in this panel bind to different epitopes found on the Tie2 ECD. See Example 9. [Figure 8] Figures 8A and 8B: Systemic administration of anti-Tie2 antibody did not appear to reduce neovascularization (NV) (Figure 8A), but reduced vaso-obliteration (VO) in an oxygen-induced retinopathy (OIR) model (Figure 8B). Each data point represents the mean of two eyes from one pup. Bars represent mean + / - SEM, N=7-8 1-way ANOVA, Tukey post-hoc |*p<0.05, **p<0.01, ***p<0.001. See Example 10. [Figure 9A] Figure 9A and Figure 9B: Anti-Tie2 antibody inhibits laser-induced choroidal neovascularization (CNV) in mouse model. Quantification of neovascularization in lesions and vascular density in retina was performed by Image J. P value was evaluated by Student's t-test (significant change, p<0.05). See Example 11. [Figure 9B] See legend to Figure 9A. [Figure 10A]Figures 10A and 10B: Schematic diagrams of Tie2 / VEGF bispecific constructs of the present invention. Figure 10A shows a schematic diagram of an anti-Tie2 antibody linked to two VEGF scFv antibody fragments via a polypeptide linker. Exemplary constructs are embodied in antibody clone #57 and antibody clone #58. Figure 10B shows a schematic diagram of two anti-Tie2 scFv antibody fragments linked to two VEGF-trap proteins composed of R1-D2 and R3-D3. Exemplary constructs are embodied in antibody clone #55 and #56. See Example 13. [Figure 10B] See legend to Figure 10A. [Figure 11] Tie2 agonism of Tie2 / VEGF bispecific constructs was evaluated by Western blot. Controls used were untreated and anti-Ang1 antibody (Ang1). Positive controls were anti-Tie2 antibody clone #3 or nonspecific antibody (NS). Test bispecific antibodies were anti-Tie2 / VEGF bispecific (antibody clone #54), or nonspecific antibody / VEGF bispecific (NS / VEGF), or VEGF-trap / aTie2 (antibody clone #55), or VEGF-trap alone. See Examples 3 and 14. [Figure 12-1] 12A-12C: Schematic diagrams of tetravalent and bi-paratopic anti-Tie2 antibody constructs of the invention for increasing valency to Tie2 or cross-linking multiple Tie2 epitopes. Exemplary tetravalent anti-Tie2 constructs with sequences of anti-Tie2 antibody heavy and light chains linked via a polypeptide linker to anti-Tie2 scFv sequences on the C-terminus of the IgG heavy chain (FIG. 12A) or the N-terminus of the heavy chain (FIG. 12B). Exemplary bi-paratopic anti-Tie2 antibody constructs with sequences of two anti-Tie2 scFvs from one antibody clone (aTie2 scFv B) linked via a polypeptide linker to the C-terminus of the IgG heavy chain of a second different anti-Tie2 antibody clone (aTie2 Fab A) (FIG. 12C). In all cases, the Tie2 binding sites are indicated by hatched boxes. See Example 15. [Figure 12-2]See description of Figure 12-1. [Figure 13] Schematic diagram of the hexamerized anti-Tie2 antibody construct of the present invention for enhancing oligomerization and thereby increasing Tie2 agonism. An exemplary hexamerized anti-Tie2 construct is shown having the heavy and light chain sequences of an anti-Tie2 antibody carrying the E430G Fc mutation. See Example 15. [Figure 14] The Tie2 agonism of the various anti-Tie2 antibody constructs of the present invention was all evaluated by pAKT / pERK Western blot. The controls used were untreated, anti-Ang1 antibody (Ang1), anti-Tie2 antibody clone #3 (aTie2), or non-specific antibody (NS). The antibody constructs tested were a hexamerized anti-Tie2 antibody with E430G Fc mutation (antibody clone #50) and its control, a non-specific hexamerized antibody (NS E430G), two tetravalent anti-Tie2 antibody constructs (antibody clones #51 and #52), and a bivalent anti-Tie2 antibody clone #2. See Examples 3, 14, and 15. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] Detailed Description of the Invention I. Definition "Tie2" is also known as angiopoietin-1 receptor, or TEK receptor tyrosine kinase, or CD202B (cluster of differentiation 202B), and is a protein encoded by the TEK gene in humans (Partanen Jet et al., (April 1992). A novel endothelial cell surface receptor tyrosine kinase with extracellular epidermal growth factor homology domains. Molecular and Cellular Biology. 12(4):1698-707). The receptor possesses a unique extracellular domain that contains three immunoglobulin-like loops, three epidermal growth factor-like repeats, and three fibronectin type III-like repeats (see Fiedler et al., 2006. Angiopoietins: A Link between Angiogenesis and Inflammation. Trends in Immunology 27(12):552-58; Barton et al., Crystal structures of the Tie2 receptor ectodomain and the angiopoietin-2-Tie2 complex. Nature Struc. & Mol. Biology, 13, pp524-532 (2006)). The contact residues of angiopoietin-1 and angiopoietin-2 are mostly overlapping on the Tie-2 receptor, as suggested by the analysis of the crystal structure of the Ang2 / Tie2 complex, and are located mainly within the second Ig-like loop (Barton et al., Nat Str Biol 2006).Other studies support the notion that the binding domains of Ang1 and Ang2 are similar or identical (Fiedler et al., Angiopoietin-1 and angiopoietin-2 share the same binding domains in the Tie-2 receptor involving the first Ig-like loop and the epidermal growth factor-like repeats. JBC. Vol. 278(3):1721-7(2003)). An exemplary amino acid sequence of human Tie2 can be found in UniProt Accession No. Q02763 (SEQ ID NO: 241).

[0011] As used herein, the term "about" refers to the normal error range for the respective value, which is readily known to one of ordinary skill in the art. Reference herein to a value or parameter with "about" includes (and describes) embodiments that are directed to the value or parameter itself.

[0012] An "acceptor human framework" for the purposes of this specification is a framework that comprises the amino acid sequence of a light chain variable domain (VL) framework or a heavy chain variable domain (VH) framework derived from a human immunoglobulin framework or a human consensus framework as defined below. An acceptor human framework "derived" from a human immunoglobulin framework or a human consensus framework may comprise the same amino acid sequence or may contain amino acid sequence changes. In some embodiments, the number of amino acid changes is 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, or 2 or less. In some embodiments, the VL acceptor human framework is identical in sequence to the VL human immunoglobulin framework sequence or the human consensus framework sequence.

[0013] "Active" or "activity" or "biological activity" in the context of the antibody of the present invention is the ability to agonize (partially or fully activate) the biological activity of its target, for example, in vitro and / or in vivo. One example of the biological activity of an antibody is the ability to achieve a measurable improvement in the state, e.g., pathology, of a disorder associated with its target. For example, with respect to an anti-Tie2 antibody, the disorder can be, for example, a Tie2-associated disorder, such as AMD (e.g., geographic atrophy). The activity of an anti-Tie2 antibody can be determined in in vitro or in vivo tests, including binding assays, activity assays (e.g., FRET-based activity assays (e.g., using H2-Opt substrate), or mass spectrometry-based activity or signaling assays), using relevant animal models or human clinical trials. The activity of an anti-Tie2 antibody of the present invention can be determined in in vitro or in vivo tests, including binding assays, alternative pathway hemolysis assays (e.g., assays that measure inhibition of alternative pathway complement activity or activation), using relevant animal models or human clinical trials.

[0014] The term "active site of Tie2" is defined as the Ang1 / Ang2 binding domain on Tie2, known to be located within the Ig2-like domain of the extracellular domain of Tie2.

[0015] "Affinity" refers to the strength of the sum of non-covalent interactions between a single binding site of a molecule (e.g., an antibody) and its binding partner (e.g., an antigen). Unless otherwise indicated, as used herein, "binding affinity" refers to the intrinsic binding affinity that reflects a 1:1 interaction between members of a binding pair (e.g., an antibody and an antigen). The affinity of a molecule X for its partner Y can generally be represented by a dissociation constant (KD). Affinity can be measured by common methods known in the art, including those described herein.

[0016] An "affinity matured" antibody refers to an antibody that has one or more modifications in one or more hypervariable regions (CDRs) and / or framework regions (FRs) compared to a parent antibody that does not possess such modifications, which improve the affinity of the antibody for antigen.

[0017] "Allosteric activation of Tie2" is the activation of Tie2 by an agonist anti-Tie2 antibody that specifically interacts with a region of Tie2 other than the described ligand binding or active site, such that binding alters Tie2 conformation or clustering and enhances receptor activity.

[0018] The term "antibody" herein is used in the broadest sense and encompasses a variety of antibody structures, including, but not limited to, monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g., bispecific antibodies), and antibody fragments, so long as they exhibit the desired antigen-binding activity.

[0019] "Antibody fragment" refers to a molecule other than an intact antibody that contains a portion of an intact antibody that binds to the antigen to which the intact antibody binds. Examples of antibody fragments include, but are not limited to, Fv, Fab, Fab', Fab'-SH, F(ab')2; diabodies; linear antibodies; single-chain antibody molecules (e.g., scFv); and multispecific antibodies formed from antibody fragments.

[0020] Papain digestion of antibodies produces two identical antigen-binding fragments called "Fab" fragments and a remaining "Fc" fragment, a designation that reflects the ability to crystallize readily. The Fab fragment consists of the entire light (L) chain along with the variable region domain (VH) of the heavy (H) chain and the first constant domain (CH1) of one heavy chain. Pepsin treatment of antibodies yields a single large F(ab')2 fragment that roughly corresponds to two disulfide-linked Fab fragments with bivalent antigen-binding activity and is still capable of cross-linking antigen. The Fab' fragment differs from the Fab fragment by having several additional residues at the carboxy terminus of the CH1 domain, including one or more cysteines from the antibody hinge region. Fab'-SH is the designation herein for Fab' in which the cysteine ​​residues of the constant domains bear a free thiol group. F(ab')2 antibody fragments were originally produced as pairs of Fab' fragments with hinge cysteines between them. Other chemical couplings of antibody fragments are also known.

[0021] The term "Fc region" herein is used to define a C-terminal region of an immunoglobulin heavy chain that includes at least a portion of the constant region. The term includes native sequence Fc regions and variant Fc regions. In one embodiment, a human IgG heavy chain Fc region extends from Cys226, or from Pro230, to the carboxyl terminus of the heavy chain. However, the C-terminal lysine (Lys447) of the Fc region may or may not be present. Unless otherwise specified herein, the numbering of amino acid residues in the Fc region or constant region is according to the EU numbering system, also referred to as the EU index, as described in Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991).

[0022] An "Fv" consists of a dimer of one heavy chain variable region domain and one light chain variable region domain in tight non-covalent association. The folding of these two domains results in six hypervariable loops (three loops from each of the H and L chains) that contribute amino acid residues for antigen binding and confer antigen-binding specificity to the antibody. However, even a single variable domain (or even half of an Fv containing only three CDRs specific for an antigen) has the ability to recognize and bind antigen, although often with a lower affinity than the entire binding site.

[0023] "Single-chain Fv", also abbreviated as "sFv" or "scFv", is an antibody fragment that contains VH and VL antibody domains connected to a single polypeptide chain. The sFv polypeptide may further contain a polypeptide linker between the VH and VL domains that allows the sFv to form the desired structure for antigen binding. For a general review of sFv, see Pluckthun in The Pharmacology of Monoclonal Antibodies, vol. 113, Rosenburg and Moore eds., Springer-Verlag, New York, pp. 269-315 (1994).

[0024] The term "diabody" refers to small antibody fragments prepared by constructing sFv fragments (see previous paragraph) with a short linker (about 5-10 residues) between the VH and VL domains such that interchain pairing is achieved rather than intrachain pairing of the V domains, resulting in a bivalent fragment, i.e., a fragment with two antigen binding sites. Bispecific diabodies are heterodimers of two "crossover" sFv fragments in which the VH and VL domains of the two antibodies are present on different polypeptide chains. Diabodies are described, for example, in EP 404,097, WO 93 / 11161, and Hollinger et al., Proc. Natl. Acad. Sci. USA, 90:6444-6448, 1993.

[0025] A "blocking" or "antagonist" antibody is one that inhibits or reduces the biological activity of the antigen to which it binds. Certain blocking or antagonist antibodies substantially or completely inhibit the biological activity of an antigen.

[0026] An "agonist" or "activating" antibody is an antibody that activates, stimulates, or increases the biological or signaling activity of the antigen to which it binds. In some situations, it is contemplated that an agonist antibody can act similarly to the way a ligand engages and activates its cognate receptor. In other situations, it is contemplated that the anti-Tie2 antibodies of the present invention are agonists if they induce Tie2 signaling, as determined by an increase in the levels of one or more of intracellular phosphorylated Tie2 (pTie2), and / or phosphorylated Akt (pAkt), and / or phosphorylated ERK (pERK), as described in Example 3. In addition, it is further contemplated that the agonist Tie2 antibodies of the present invention can also activate downstream signaling of their target antigen in the presence or absence of endogenous activating (i.e., Ang1) or inhibitory (i.e., Ang2) ligands, as described in Examples 6 and 7.

[0027] An "antibody that binds to the same epitope" as a reference antibody refers to an antibody that contacts an overlapping set of amino acid residues of an antigen, or blocks the binding of the reference antibody to the antigen by 50% or more in a competitive assay, compared to the reference antibody. The amino acid residues of an antibody that contact an antigen can be determined, for example, by determining the crystal structure of the antibody in complex with the antigen, or by performing hydrogen / deuterium exchange. In some embodiments, the residues of an antibody that are within 5 angstroms of the antigen are considered to contact the antigen. In some embodiments, an antibody that binds to the same epitope as a reference antibody blocks the binding of the reference antibody to the antigen by 50% or more in a competitive assay, and conversely, the reference antibody blocks the binding of the antibody to the antigen by 50% or more in a competitive assay. Exemplary competitive assays are provided herein.

[0028] As used herein, the term "biparatopic" refers to a bispecific antibody in which a first antigen-binding moiety and a second antigen-binding moiety bind to different epitopes on the same antigen.

[0029] The term "chimeric" antibody refers to an antibody in which a portion of the heavy and / or light chain is derived from a particular source or species, while the remainder of the heavy and / or light chain is derived from a different source or species.

[0030] The "class" of an antibody refers to the type of constant domain or constant region carried by its heavy chain. There are five major classes of antibodies: IgA, IgD, IgE, IgG, and IgM, and some of these can be further divided into subclasses (isotypes), e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2. The heavy chain constant domains corresponding to the different classes of immunoglobulins are called α, δ, ε, γ, and μ, respectively.

[0031] "Complement factors" refers to various proteins and glycoproteins that make up the complement cascade, which is part of the immune system, enhancing (complementing) the ability of antibodies and phagocytes to eliminate microorganisms and damaged cells from the organism, promote inflammation, and attack the cell membranes of pathogens. It is part of the innate immune system. Complement factors contemplated herein include, for example, C1, C2, C2a, C2b, C3, C3a, C3b, C4, C4a, C4b, C5, C5a, C5b, C6, C7, C8, and C9.

[0032] "Effector function" refers to the biological activities attributable to the Fc region of an antibody, which vary depending on the antibody isotype. Examples of antibody effector functions include C1q binding and complement-dependent cytotoxicity (CDC), Fc receptor binding, antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, down-regulation of cell surface receptors (e.g., B cell receptors), and B cell activation.

[0033] "Framework" or "Framework region" or "FR" refers to variable domain residues other than the hypervariable region (CDR) residues. The FR of a variable domain generally consists of four FR domains: FR1, FR2, FR3, and FR4.

[0034] The terms "full length antibody," "intact antibody," and "whole antibody" are used interchangeably herein and refer to an antibody having a structure substantially similar to a native antibody structure or having a heavy chain that includes an Fc region as defined herein.

[0035] The term "fusion polypeptide" encompasses, for example, an anti-Tie2 antibody of the present invention fused to an immunoglobulin Fc region. The Fc region may, for example, comprise an immunoglobulin CH3 domain, which may be naturally occurring or modified in some way. Such Fc fusion polypeptides may exhibit a higher half-life in vivo than their non-fused counterparts. The fusion to an Fc region also allows for dimerization / multimerization of the fusion polypeptide. As contemplated, the Fc region may be a naturally occurring Fc region or may be modified to improve certain qualities, such as, for example, therapeutic qualities, circulation time, reduced aggregation issues, etc. In another embodiment, the fusion polypeptide contemplates an anti-Tie2 antibody fragment fused to a ligand, such as, for example, Ang1, to enhance the agonist activity of the fusion polypeptide. In another embodiment, the fusion polypeptide contemplates an anti-Tie2 antibody fragment fused to, for example, a cytokine to induce other desired biology.

[0036] As used herein, a "hexamerizing antibody" is one in which the introduction of the E430G mutation in the Fc region promotes the natural process of antibody hexamer formation by increasing intermolecular Fc-Fc interactions upon binding to membrane-bound antigens on the cell surface (Diebolder et al., Science. 2014; de Jong et al., PLoS Biol. 2016).

[0037] A "human antibody" is one that possesses an amino acid sequence that corresponds to that of an antibody produced by a human or human cell, or derived from a non-human source that utilizes the human antibody repertoire or other human antibody-encoding sequences. This definition of a human antibody specifically excludes humanized antibodies, which contain non-human antigen-binding residues.

[0038] A "human consensus framework" is a framework that represents the most commonly occurring amino acid residues in a selection of human immunoglobulin VL or VH framework sequences. Generally, the selection of human immunoglobulin VL or VH sequences is from a subgroup of variable domain sequences. Generally, the subgroup of sequences is a subgroup as in Kabat et al., Sequences of Proteins of Immunological Interest, Fifth Edition, NIH Publication 91-3242, Bethesda Md. (1991), vols. 1-3. In one embodiment, for VL, the subgroup is subgroup kappa I as in Kabat et al., supra. In one embodiment, for VH, the subgroup is subgroup III as in Kabat et al., supra.

[0039] "Humanized" forms of non-human (e.g., rodent) antibodies are chimeric antibodies that contain minimal sequences derived from the non-human antibody. In most cases, humanized antibodies are human immunoglobulins (recipient antibody) in which residues from a hypervariable region of the recipient are replaced by residues from a hypervariable region of a non-human species (donor antibody) such as mouse, rat, rabbit, or non-human primate having the desired antibody specificity, affinity, and capacity. In some cases, FR residues of the human immunoglobulin are replaced by corresponding non-human residues. Furthermore, humanized antibodies may contain residues that are not found in the recipient antibody or the donor antibody. These modifications are made to further improve antibody performance. In general, a humanized antibody will contain substantially all of at least one, and typically two, variable domains, in which all or substantially all of the hypervariable loops correspond to those of a non-human immunoglobulin and all or substantially all of the FRs are those of a human immunoglobulin sequence. The humanized antibody optionally also will comprise at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin. For details, see Jones et al., Nature 321:522-525 (1986), Riechmann et al., Nature 332:323-329 (1988), and Presta, Curr Op. Struct. Biol. 2:593-596 (1992).

[0040] The term "variable" refers to the fact that certain segments of the variable domains differ widely in sequence among antibodies. The variable or "V" domains mediate antigen binding and define the specificity of a particular antibody for its particular antigen. However, the variability is not uniformly distributed across the span of the variable domain. Instead, the V regions consist of relatively invariant stretches of 15-30 amino acids called framework regions (FRs) separated by shorter regions of extreme variability called "hypervariable regions", each 9-12 amino acids long. As used herein, the terms "hypervariable region" or "CDR" refer to the amino acid residues of an antibody that are responsible for antigen binding. The hypervariable regions generally comprise, for example, amino acid residues from about residues 24-34 (L1), 50-56 (L2), and 89-97 (L3) in the VL, and from about residues 26-35 (H1), 49-65 (H2), and 95-102 (H3) in the VH (in one embodiment, H1 is from about residues 31-35) (Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991)), and / or amino acid residues from the "hypervariable loops" (e.g., in VL, residues 26-32 (L1), 50-52 (L2), and 91-96 (L3), and in VH, residues 26-32 (H1), 53-55 (H2), and 96-101 (H3)) (Chothia and Lesk, J. Mol. Biol. 196:901-917 (1987)). Naturally occurring heavy and light chain variable domains each contain four FRs, which adopt a primarily beta-sheet configuration, connected by three hypervariable regions that form loops connecting, and in some cases forming part of, the beta-sheet structure.The hypervariable regions of each chain are held together in close proximity by the FRs and, together with the hypervariable regions from the other chain, contribute to the formation of the antigen-binding site of the antibody (see Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991)). Thus, the CDR and FR sequences are generally within the following sequence in VH (or VL): FR1-H1(L1)-FR2-H2(L2)-FR3-H3(L3)-FR4. The constant domains are not directly involved in binding the antibody to the antigen, but exhibit various effector functions, such as participating in antibody-dependent cellular cytotoxicity (ADCC).

[0041] The terms "residue numbering such as Kabat numbering", "Kabat amino acid residue", or "amino acid position numbering such as Kabat numbering", and variations thereof, refer to the numbering system used for the heavy or light chain variable domains of the antibody compilations in Kabat et al., supra. Using this numbering system, the actual linear amino acid sequence may contain fewer or additional amino acids corresponding to shortening or insertion into the FR or CDR of the variable domain. For example, the heavy chain variable domain may contain a single amino acid insertion after residue 52 of H2 (residue 52a according to Kabat) and inserted residues after heavy chain FR residue 82 (e.g., residues 82a, 82b, and 82c according to Kabat, etc.). The Kabat residue numbering may be determined for a given antibody by alignment of the antibody's sequence at the region of homology with the "standard" Kabat numbered sequence.

[0042] The Kabat numbering system is generally used when referring to residues in the variable domain (approximately residues 1-107 of the light chain and residues 1-113 of the heavy chain) (see, e.g., Kabat et al., Sequences of Immunological Interest. 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991)). The "EU numbering system" or "EU index" is generally used when referring to residues in the immunoglobulin heavy chain constant region (e.g., the EU index reported in Kabat et al., supra). The "EU index, such as Kabat numbering" refers to the residue numbering of a human IgG1 EU antibody. Unless otherwise stated herein, references to residue numbers in the variable domain of an antibody refer to the residue numbering according to the Kabat numbering system. Unless otherwise stated herein, references to residue numbers in the constant domain of an antibody refer to the residue numbering according to the EU numbering system. Unless otherwise indicated, CDR residues and other residues in the variable domain (eg, FR residues) are numbered herein according to Kabat et al., supra.

[0043] An "immunoconjugate" is an antibody conjugated to one or more heterologous molecules that deliver a cell-killing or cell-modifying activity, including, but not limited to, a small molecule drug (inhibitor or activator), or a cytotoxic agent, e.g., a chemotherapeutic agent or drug, a growth inhibitory agent, a toxin (e.g., a protein toxin, an enzymatically active toxin of bacterial, fungal, plant, or animal origin, or fragments thereof), or a radioisotope.

[0044] When used to describe various antibodies disclosed herein, the term "isolated antibody" refers to an antibody that has been identified and separated and / or recovered from the cell or cell culture in which it is expressed. Contaminant components of its natural environment are typically substances that would interfere with diagnostic or therapeutic uses of the polypeptide and may include enzymes, hormones, and other proteinaceous or non-proteinaceous solutes. In some embodiments, the antibody is purified to greater than 95% or 99% purity, as determined, for example, by electrophoretic (e.g., SDS-PAGE, isoelectric focusing (IEF), capillary electrophoresis) or chromatographic (e.g., ion exchange or reverse-phase HPLC) approaches. For a review of methods for assessing antibody purity, see, for example, Flatman et al., J. Chromatogr. B 848:79-87 (2007). In certain embodiments, the antibody will be purified (1) sufficiently to obtain at least 15 residues of N-terminal or internal amino acid sequence using a spinning cup sequencer, or (2) to homogeneity by SDS-PAGE under non-reducing or reducing conditions using Coomassie blue or silver staining. An isolated antibody includes an antibody in situ within a recombinant cell, since at least one component of the polypeptide's natural environment will not be present. Ordinarily, however, an isolated polypeptide will be prepared by at least one purification step.

[0045] As used herein, the term "monoclonal antibody" refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies constituting the population are identical and / or bind the same epitope, except for possible variant antibodies, e.g., including naturally occurring mutations or arising during the production of the monoclonal antibody preparation, and such variants are generally present in minor amounts. In contrast to polyclonal antibody preparations, which typically contain different antibodies directed against different determinants (epitopes), each monoclonal antibody of a monoclonal antibody preparation is directed against a single determinant on an antigen. Thus, the modifier "monoclonal" indicates the character of the antibody obtained from a substantially homogeneous antibody population and should not be interpreted as requiring the production of the antibody by any method. For example, the monoclonal antibodies used in accordance with the present invention may be produced by a variety of techniques, including, but not limited to, hybridoma methods, recombinant DNA methods, phage display methods, and methods utilizing transgenic animals containing all or part of the human immunoglobulin locus, and such methods and other exemplary methods for producing monoclonal antibodies are described herein.

[0046] The term "multispecific antibody" is used in the broadest sense and specifically encompasses antibodies or antibody fragments with polyepitopic specificity (i.e., capable of binding to two different epitopes on one biological molecule or to respective epitopes on different biological molecules). Such multispecific antibodies include, but are not limited to, full-length antibodies, antibodies with two or more VL and VH domains, antibody fragments, such as Fab, Fv, dsFv, scFv, diabodies, bispecific diabodies and triabodies, covalently or non-covalently linked antibody fragments. "Multiple epitopic specificity" refers to the ability to specifically bind to two or more different epitopes on the same or different targets. "Dual specificity" or "bispecificity" refers to the ability to specifically bind to two different epitopes on the same or different targets. However, in contrast to bispecific antibodies, dual-specific antibodies have two antigen-binding arms that are identical in amino acid sequence, and each Fab arm can recognize two antigens. Dual-specificity allows an antibody to interact with two different antigens with high affinity as a single Fab or IgG molecule. According to one embodiment, a multispecific antibody in IgG1 format binds to each epitope with an affinity of 5 μM-0.001 pM, 3 μM-0.001 pM, 1 μM-0.001 pM, 0.5 μM-0.001 pM, or 0.1 μM-0.001 pM. "Monospecific" refers to an antibody that has the ability to bind only one epitope on a particular antigen.

[0047] With respect to the binding of an antibody to a target molecule, the term "specific binding" to an epitope on a particular polypeptide or a particular polypeptide target, or "specifically binds to" or "is specific for" an epitope on a particular polypeptide or a particular polypeptide target, refers to binding that is measurably different from non-specific interactions. Specific binding can be measured, for example, by determining the binding of a molecule compared to the binding of a control molecule. For example, specific binding can be determined by competition with a control molecule similar to the target, for example, an excess of unlabeled target. In this case, specific binding is indicated when the binding of the labeled target to the probe is competitively inhibited by an excess of unlabeled target. As used herein, the term "specific binding" to an epitope on a particular polypeptide or a particular polypeptide target, or "specifically binds to" or "is specific for" an epitope on a particular polypeptide or a particular polypeptide target, refers to binding that is measurably different from non-specific interactions. -4 M or less, alternatively 10 -5 M or less, alternatively 10 -6 M or less, alternatively 10 -7 M or less, alternatively 10 -8 M or less, alternatively 10 -9 M or less, alternatively 10 -10 M or less, alternatively 10 -11 M or less, alternatively 10 -12 KD for targets below M, or 10 -4 M~10 -6 M or 10 -6 M~10 -10 M or 10 -7 M~10 -9 The affinity can be shown by a molecule having a KD in the range of M. As understood by those skilled in the art, affinity and KD value are inversely related. High affinity for an antigen is measured by a low KD value. In one embodiment, the term "specific binding" refers to the binding of a molecule such as the antibody of the present invention to a specific polypeptide or an epitope on a specific polypeptide without substantially binding to any other polypeptide or polypeptide epitope.

[0048] The term "non-ligand competitive binder" refers to an anti-Tie2 antibody of the present invention that does not compete with either Ang1 or Ang2 for the active site of Tie2, while still allowing either Ang1 or Ang2 to bind at the active site.

[0049] "Antibody-encoding nucleic acid" refers to one or more nucleic acid molecules encoding antibody heavy and light chains (or fragments thereof), including such nucleic acid molecules in a single vector or separate vectors, and which are present at one or more locations within a host cell. In some embodiments, the nucleic acid encodes an anti-Tie2 antibody.

[0050] The term "vector," as used herein, refers to a nucleic acid molecule capable of propagating another nucleic acid to which it is linked. The term includes vectors as self-replicating nucleic acid structures and vectors that integrate into the genome of a host cell into which they are introduced. Certain vectors are capable of directing the expression of nucleic acids to which they are operatively linked. Such vectors are referred to herein as "expression vectors."

[0051] The terms "host cell," "host cell line," and "host cell culture" are used interchangeably and refer to cells into which exogenous nucleic acid has been introduced, including the progeny of such cells. Host cells include "transformants" and "transformed cells," which include the primary transformed cell and progeny derived therefrom regardless of the number of passages. The progeny may not be completely identical to the parent cell in nucleic acid content, but may contain mutations. Mutant progeny that have the same function or biological activity as screened or selected for in the originally transformed cell are included herein.

[0052] "Percentage (%) of amino acid sequence identity" with respect to a reference polypeptide sequence is defined as the percentage of amino acid residues in a candidate sequence that are identical to the amino acid residues in the reference polypeptide sequence, without considering any conservative substitutions as part of sequence identity, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity. Alignment for the purpose of determining percent amino acid sequence identity can be achieved in a variety of ways that are within the skill of the art, for example, using publicly available computer software such as BLAST, BLAST-2, ALIGN or Megalign (DNASTAR) software. Those skilled in the art can determine the appropriate parameters for aligning sequences, including any algorithms required to achieve maximum alignment over the entire length of the sequences to be compared.

[0053] Proteins, including antibodies, are said to be "stable" when they retain essentially intact conformation and biological activity.Various analytical techniques for measuring protein stability are available in the art, and are reviewed in, for example, Peptide and Protein Drug Delivery, 247-301, Vincent Lee Ed., Marcel Dekker, Inc., New York, NY, Pubs. (1991) and Jones (1993) Adv. Drug Delivery Rev. 10: 29-90.Antibody variants with "improved stability" refer to antibody variants that are more stable compared to the starting reference antibody.Antibody variants with improved stability are variants of reference (wild type) antibodies in which certain amino acid residues are modified to improve the physical stability, and / or chemical stability, and / or biological activity of natural antibodies, and / or to reduce immunogenicity.

[0054] In certain embodiments, anti-Tie2 antibodies can be used as therapeutic agents to target and interfere with diseases or conditions in which Tie2 activity is involved. Antibodies can also be subjected to other biological activity assays, for example, to evaluate their effectiveness as therapeutic agents. Such assays are known in the art and depend on the target antigen and the intended use for the antibody. Examples include HUVEC inhibition assays, tumor cell growth inhibition assays (e.g., as described in WO89 / 06692), antibody-dependent cellular cytotoxicity (ADCC) and complement-mediated cytotoxicity (CDC) assays (US Pat. No. 5,500,362), and agonist activity or hematopoietic assays (see WO95 / 27062).

[0055] As used herein, the term "trispecific" refers to a type of antibody that possesses three antigen recognition and binding sites, some of which may bind to Tie2. In other cases, at least one binding arm specifically binds to Tie2, and the other binding site may bind either to Tie2 or another antigen of interest (listed under "bispecific antibodies"). In one embodiment, such a trispecific antibody comprises an antibody fragment (e.g., Fab, scFv, single domain antibody). As a non-limiting example, three antibody binding fragments of the present invention may be assembled into a trispecific antibody, such that at least one antibody binding fragment binds to Tie2 and the remaining antibody binding fragment binds to another antigen, such as, for example, VEGF. See Runcie et al., Bi-specific and Tri-specific antibodies - the next big thing in solid tumor therapeutics., Mol.Med., 24, (50) (2018).

[0056] As used herein, the term "tetraspecific" refers to a type of antibody that possesses four antigen recognition and binding sites, some of which may bind to Tie2. In other cases, at least one binding arm specifically binds to Tie2, and the other binding site may bind either to Tie2 or another antigen of interest (listed under "bispecific antibodies"). In one embodiment, such a tetraspecific antibody comprises an antibody fragment (e.g., Fab, scFv, single domain antibody). As a non-limiting example, four antibody binding fragments of the present invention may be assembled into a tetraspecific antibody, such that two antibody binding fragments bind to Tie2 and the other two antibody binding fragments bind to another antigen, such as, for example, VEGF.

[0057] As used herein, the term "pentaspecific" refers to a type of antibody that possesses five antigen recognition and binding sites, some of which may bind to Tie2. In other cases, at least one binding arm specifically binds to Tie2, and the other binding site may bind either to Tie2 or another antigen of interest (listed under "bispecific antibodies"). In one embodiment, such a pentaspecific antibody comprises an antibody fragment (e.g., Fab, scFv, single domain antibody). As a non-limiting example, five antibody binding fragments of the present invention may be assembled into a pentaspecific antibody, such that at least one antibody binding fragment binds to Tie2, and the other antibody binding fragment binds to another antigen, such as, for example, VEGF.

[0058] As used herein, the term "hexaspecific" refers to a type of antibody that possesses six antigen recognition and binding sites, some of which may bind to Tie2. In other cases, at least one binding arm specifically binds to Tie2, and the other binding site may bind either to Tie2 or another antigen of interest (listed under "bispecific antibodies"). In one embodiment, such a hexaspecific antibody comprises an antibody fragment (e.g., Fab, scFv, single domain antibody). As a non-limiting example, six antibody binding fragments of the present invention may be assembled into a hexaspecific antibody, such that at least one antibody binding fragment binds to Tie2 and the other antibody binding fragment binds to another antigen, such as, for example, VEGF.

[0059] A "polypeptide linker" as used herein is a polypeptide comprising two or more amino acid residues joined by peptide bonds that is used to link two polypeptides (e.g., a VH domain and a VL domain, two scFv antibody fragments, or a variable domain and an extracellular trap protein, or an scFv antibody fragment and an extracellular trap protein). The linker may be flexible or rigid / inflexible. Examples of such linker polypeptides are well known in the art (see, e.g., Hollinger P, et al., PNAS USA. 90:6444-6448 (1993); Poljak RJ. Structure 2:1121-1123 (1994)). Non-limiting examples of suitable non-immunogenic linker peptides are the (G4S)n, (SG4)n or G4(SG4)n flexible peptide linkers, or the rigid / inflexible linkers (EAAAK)n or (XP)n (in each case "n" is a number from 1 to 10, or 1 to 4), and oligomers of such linkers.

[0060] A "Tie2 dysregulated disease" is any condition that would benefit from treatment with the anti-Tie2 antibody of the present invention. Non-limiting examples of diseases treated herein include, but are not limited to, any disease or disorder resulting from an imbalance or disruption of the interaction between Ang-1, Ang-2, Ang-3, or Ang-4 and Tie2. Non-limiting examples may include, for example, infectious diseases, acute respiratory distress syndrome (ARDS), ischemic injury, eye damage, radiation injury, cancer, systemic sclerosis, traumatic brain injury, radiation injury, wound healing, myocardial infarction, blood-brain barrier damage (i.e., Alzheimer's disease or other neurodegenerative diseases), neuroinflammation, cerebral cavernous malformation, Duchenne muscular dystrophy (DMD) or Clarkson's disease. In one embodiment, the Tie2 dysregulated infectious disease includes sepsis, dengue virus infection, tuberculosis, or influenza. In another embodiment, the Tie2 dysregulated disease may include, for example, diabetic nephropathy, acute kidney injury, chronic kidney disease, kidney or other organ transplant, critical limb ischemia, traumatic brain injury, or ischemic injury such as stroke. In another embodiment, the Tie2 dysregulated eye disorder may include, for example, diabetic retinopathy, diabetic macular edema (DME), proliferative diabetic retinopathy (PDR), age-related macular degeneration (AMD), retinopathy of prematurity (ROP), or glaucoma.

[0061] As used herein, "administering" refers to a method of administering a dosage of a therapeutic agent (e.g., an anti-Tie2 antibody of the invention, a nucleic acid encoding an anti-Tie2 antibody of the invention) or composition (e.g., a pharmaceutical composition, e.g., a pharmaceutical composition comprising an anti-Tie2 antibody of the invention) to a subject in need thereof. The compositions utilized in the methods described herein can be administered, for example, intravitreally (e.g., by intravitreal injection), ocularly (e.g., by intraocular injection), intraocularly (e.g., by intraocular injection), subcutaneously, or intravenously. The compositions utilized in the methods described herein can also be administered systemically or locally. The method of administration can vary depending on various factors (e.g., the compound or composition being administered and the severity of the condition, disease, or disorder being treated).

[0062] As used herein, "co-administration" means administration of two or more separate therapeutic agents (e.g., an anti-Tie2 antibody of the invention and an anti-VEGF antibody therapeutic or recombinant VEGF fusion protein therapeutic) or compositions (e.g., an anti-Tie2 antibody pharmaceutical composition of the invention and an anti-VEGF antibody composition or recombinant VEGF fusion protein composition) concurrently or at the same time to a subject in need thereof.

[0063] As used herein, "co-formulation" means two or more separate therapeutic agents (e.g., an anti-Tie2 antibody of the invention and an anti-VEGF antibody therapeutic or a recombinant VEGF fusion protein therapeutic) or compositions (e.g., an anti-Tie2 antibody pharmaceutical composition of the invention and an anti-VEGF antibody composition or a recombinant VEGF fusion protein composition) that are combined into a single formulation to be administered to a subject in need.

[0064] An "individual" or "subject" is a mammal. Mammals include, but are not limited to, domestic animals (e.g., cows, sheep, cats, dogs, and horses), primates (e.g., humans and non-human primates such as monkeys), rabbits, and rodents (e.g., mice and rats). In certain embodiments, an individual or subject is a human. A "subject" may be a "patient."

[0065] As used herein, "treatment" (and "treat" or "treating") refers to clinical intervention in an attempt to modify the natural course of the individual being treated, and may be performed for prophylaxis or during the course of clinical pathology. Desirable effects of treatment include, but are not limited to, prevention of recurrence of the disease or disorder, alleviation of symptoms, reduction of any direct or indirect pathological consequences of the disease or disorder, reduction in the rate of disease progression, improvement or mitigation of the condition of the disease or disorder, and remission or improved prognosis. In some embodiments, the antibodies of the present invention are used to delay the onset of a disease or disorder or to slow the progression of a disease or disorder.

[0066] As used herein, the terms "cell", "cell line" and "cell culture" are used interchangeably, and all such designations include progeny. Thus, the terms "transformant" and "transformed cell" include the primary subject cell and culture derived, regardless of the number of transfers. It is also understood that all progeny may not be exactly identical in DNA content due to deliberate or accidental mutations. Mutant progeny that have the same function or biological activity as screened for in the originally transformed cell are included. Where separate designations are intended, it will be clear from the context.

[0067] A "mutation" is a deletion, insertion, or substitution of nucleotides relative to a reference nucleotide sequence, such as a wild-type sequence.

[0068] A "variant" or "mutant" of a starting or reference polypeptide (e.g., a reference antibody or its variable domain / CDR) is a polypeptide that (1) has an amino acid sequence different from that of the starting or reference polypeptide, and (2) is derived from the starting or reference polypeptide through either natural or artificial (man-made) mutagenesis. Such variants include, for example, deletion of residues from within the amino acid sequence of the polypeptide of interest, and / or insertion of residues, and / or substitution of residues, referred to herein as "amino acid residue modifications." Thus, a variant CDR refers to a CDR that comprises a variant sequence with respect to the starting or reference polypeptide sequence (such as that of a source antibody or antigen-binding fragment). Amino acid residue modifications, in this context, refer to amino acids that differ from the amino acids at the corresponding positions in the starting or reference polypeptide sequence (e.g., the sequence of a reference antibody or its fragment). Any combination of deletion, insertion, and substitution may be made to arrive at the final variant or mutant construct, provided that the final construct possesses the desired functional characteristics. Amino acid changes may also modify post-translational processes of the polypeptide, such as changing the number or position of glycosylation sites.

[0069] As used herein, "VEGF extracellular trap protein" or "VEGF-trap" is otherwise known as aflibercept (Eylea®, Regeneron-Bayer HealthCare, Tarrytown, NY, US). It consists of ligand-binding elements derived from the extracellular components of VEGF receptors 1 and 2 fused to the Fc portion of IgG1. It binds with high affinity to VEGF-A as well as all isoforms of VEGF-B and placenta growth factor (PlGF), essentially rendering the VEGF-A and PlGF ligands unable to bind and activate the cellular receptor.

[0070] A "wild type (WT)" or "reference" sequence, i.e., a "wild type" or "reference" protein / polypeptide sequence, such as a CDR or variable domain of a reference antibody, may be a reference sequence from which a variant polypeptide is derived through the introduction of mutations. In general, the "wild type" sequence for a given protein is the sequence that is most common in nature. Similarly, a "wild type" gene sequence is the sequence of that gene that is most commonly found in nature. Mutations can be introduced into a "wild type" gene (and thus the protein that it encodes) through natural processes or through human-induced means. The product of such a process is a "variant" or "mutant" form of the original "wild type" protein or gene.

[0071] "Reference antibody" as used herein refers to an antibody or fragment thereof whose antigen-binding sequence serves as a template sequence against which diversification is performed according to the criteria described herein. The antigen-binding sequence generally includes an antibody variable region, including framework regions, at least one CDR.

[0072] Compositions and methods The present invention provides novel antibodies that bind to Tie2, and methods for making and using them, for example, for therapeutic and diagnostic applications. The antibodies of the present invention are useful, for example, for the diagnosis or treatment of various disorders, including Tie2 dysregulation diseases described herein.

[0073] The techniques and procedures described or referenced herein are generally well understood and may be found, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual 3d edition (2001) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, Current Protocols in Molecular Biology (FMA Usubel, et al. eds., (2003)), the series Methods in Enzymology (Academic Press, Inc.): PCR 2: A Practical Approach (MJ MacPherson, BD Hames and GR Taylor eds. (1995)), Harlow and Lane, eds. (1988) Antibodies, A Laboratory Manual, and Animal Cell Culture (RI Freshney, ed. (1987)), Oligonucleotide Synthesis (MJ Gait, ed., 1984), Methods in Molecular Biology, Humana Press, Cell Biology: A Laboratory Notebook (JECellis, ed., 1998) Academic Press, Animal Cell Culture (RIFreshney), ed., 1987), Introduction to Cell and Tissue Culture (JP Mather and PE Roberts, 1998) Plenum Press, Cell and Tissue Culture: Laboratory Procedures (A. Doyle, JBGriffiths, and DG Newell, eds., 1993-8) J. Wiley and Sons, Handbook of Experimental Immunology (DM Weir and CC Blackwell, eds.), Gene Transfer Vectors for Mammalian Cells (JMMiller and Μ.P.Calos, eds., 1987), PCR: The Polymerase Chain Reaction, (Mullis et al., eds., 1994), Current Protocols in Immunology (JEColigan et al., eds., 1991), Short Protocols in Molecular Biology (Wiley and Sons, 1999), Immunobiology (CA Janeway and P. Travers, 1997), Antibodies (P. Finch, 1997), Antibodies: A Practical Approach (D. Catty., ed., IRL Press, 1988-1989), Monoclonal Antibodies: A Practical Approach (P. Shepherd and C. Dean, eds., Oxford University Press, 2000), Using Antibodies: A Laboratory Manual (E. Harlow and D.Lane(Cold Spring Harbor Laboratory These methods are commonly used using conventional methodologies by those skilled in the art, such as the widely used methodologies described in The Antibodies (M. Zanetti and JD Capra, eds,,Harwood Academic Publishers,1995).

[0074] The anti-Tie2 antibodies of the invention described herein, and any antibodies for use in the methods described herein, may have any of the characteristics described herein, either alone or in combination.

[0075] In certain embodiments, the anti-Tie2 antibodies provided herein have a concentration of about 1 μM, about 100 nM, about 10 nM, about 1 nM, about 0.1 nM, about 0.01 nM, or about 0.001 nM (e.g., 10 -8 M or less, e.g., 10 -8 M~10 -13 M, for example, 10-9 M~10 -13For example, in some cases, the antibodies provided herein bind to human Tie2 (huTie2) with a KD of about 10 nM or less. In some cases, the antibodies provided herein bind to huTie2 with a KD of about 5 nM or less. In some cases, the antibodies provided herein bind to huTie2 with a KD of about 2 nM or less. For example, in some instances, the antibody may be present at a concentration of about 25 pM to about 2 nM (e.g., about 25 pM, about 50 pM, about 75 pM, about 100 pM, about 125 pM, about 150 pM, about 175 pM, about 200 pM, about 225 pM, about 250 pM, about 275 pM, about 300 pM, about 325 pM, about 350 pM, about 375 pM, about 400 pM, about 425 pM, about 450 pM, about 475 pM, about 500 pM, about 525 pM, about 550 pM, about 575 pM, about 600 pM, , about 625 pM, about 650 pM, about 675 pM, about 700 pM, about 725 pM, about 750 pM, about 775 pM, about 800 pM, about 825 pM, about 850 pM, about 875 pM, about 900 pM, about 925 pM, about 950 pM, about 975 pM, about 1 nM, about 1.1 nM, about 1.2 nM, about 1.3 nM, about 1.4 nM, about 1.5 nM, about 1.6 nM, about 1.7 nM, about 1.8 nM, about 1.9 nM, or about 2 nM). In some cases, the antibody binds to huTie2 with a KD of about 75 pM to about 600 pM (e.g., about 75 pM, about 100 pM, about 125 pM, about 150 pM, about 175 pM, about 200 pM, about 225 pM, about 250 pM, about 275 pM, about 300 pM, about 325 pM, about 350 pM, about 375 pM, about 400 pM, about 425 pM, about 450 pM, about 475 pM, about 500 pM, about 525 pM, about 550 pM, about 575 pM, about 600 pM). In some cases, the antibody binds to huTie2 with a KD of about 75 pM to about 500 pM. In some cases, the antibody binds to huTie2 with a KD of about 75 pM to about 400 pM. In some cases, the antibody binds to huTie2 with a KD of about 75 pM to about 300 pM. In some cases, the antibody binds to huTie2 with a KD of about 75 pM to about 200 pM. In some cases, the antibody binds to huTie2 with a KD of about 75 pM to about 150 pM.In some cases, the antibody binds to huTie2 with a KD of about 75 pM to about 125 pM. In some cases, the antibody binds to huTie2 with a KD of about 75 pM to about 100 pM. In some cases, the antibody binds to huTie2 with a KD of about 80 pM. In some cases, the antibody binds to huTie2 with a KD of about 60 pM. In some cases, the antibody binds to huTie2 with a KD of about 40 pM.

[0076] In one embodiment, KD is measured by radiolabeled antigen binding assay (RIA). In one embodiment, an RIA is performed with a Fab version of the antibody of interest and its antigen. For example, the solution binding affinity of the Fab for the antigen is determined by binding the Fab to a minimal concentration of ( 125 I) equilibration with labeled antigen and then measurement by capturing bound antigen with a plate coated with anti-Fab antibody (e.g., Chen et al., J. Mol. Biol. 293:865-881 (1999)). To establish conditions for the assay, MICROTITER™ multiwell plates (Thermo Scientific) are coated overnight with 5 μg / ml of capture anti-Fab antibody (Cappel Labs) in 50 mM sodium carbonate (pH 9.6), followed by blocking with 2% (w / v) bovine serum albumin in PBS for 2-5 hours at room temperature (approximately 23°C). In non-adsorbent plates (Nunc #269620), 100 pM or 26 pM [ 125I]Antigen is mixed with serial dilutions of the Fab of interest (see Presta et al., Cancer Res. 57:4593-4599 (1997)). The Fab of interest is then incubated overnight, although incubation may continue for longer periods (e.g., about 65 hours) to ensure equilibrium is reached. The mixture is then transferred to a capture plate and incubated at room temperature (e.g., 1 hour). The solution is then removed and the plate is washed 8 times with 0.1% polysorbate 20 (TWEEN-20™) in PBS. Once the plate has dried, 150 μl / well of scintillant (MICROSCINT-20™, Packard) is added and the plate is counted for 10 minutes on a TOPCOUNT™ gamma counter (Packard). The concentration of each Fab that gives 20% or less of maximum binding is selected for use in competitive binding assays.

[0077] According to another embodiment, KD is measured using BIACORE™ surface plasmon resonance (SPR) assay. For example, the assay using BIACORE™-2000 or BIACORE™-3000 (BIAcore, Inc., Piscataway, NJ) is performed at 25° C. with the antigen CM5 chip immobilized at about 10 response units (RU). In one embodiment, a carboxymethylated dextran biosensor chip (CM5, BIAcore, Inc.) is activated with N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) according to the supplier's instructions. The antigen is diluted to 5 μg / ml (about 0.2 μM) with 10 mM sodium acetate, pH 4.8, and then injected at a flow rate of 5 μl / min to achieve about 10 response units (RU) of the coupling protein. After antigen injection, 1 M ethanolamine is injected to block unreacted groups. For kinetic measurements, two-fold serial dilutions of Fab (0.78 nM to 500 nM) are injected in PBS containing 0.05% polysorbate 20 (TWEEN™-20) surfactant (PBST) at a flow rate of approximately 25 μl / min at 25° C. The association rate (kon ) and dissociation rate (k off ) is calculated using a simple one-to-one Langmuir binding model (BIACORE™ Evaluation Software version 3.2) by simultaneously fitting the association and dissociation sensorgrams. The equilibrium dissociation constant (KD) is calculated as k off / k on The on-rate is calculated as the ratio of 10 to 10. See, for example, Chen et al., J. Mol. Biol. 293:865-881 (1999). 6 M -1 s -1 If the on-rate exceeds , the on-rate can be determined using a fluorescence quenching technique that measures the increase or decrease in fluorescence emission intensity (excitation = 295 nm, emission = 340 nm, 16 nm bandpass) of 20 nM anti-antigen antibody (Fab form) in PBS (pH 7.2) at 25 °C in the presence of increasing concentrations of antigen as measured with a spectrometer such as a stopped-flow equipped spectrometer with a stirred cuvette (Aviv Instruments) or an 8000 series SLM-AMINCO™ spectrophotometer (ThermoSpectronic). KD may also be measured using the BIACORE™ SPR assay known in the art.

[0078] In certain embodiments, the antibody provided herein is an antibody fragment. Antibody fragments include, but are not limited to, Fab, Fab', Fab'-SH, F(ab')2, Fv, and scFv fragments, as well as other fragments described below. For a review of certain antibody fragments, see Hudson et al., Nat.Med.9:129-134(2003). For a review of scFv fragments, see, for example, Pluckthun, in The Pharmacology of Monoclonal Antibodies, vol.113, Rosenburg and Moore eds., (Springer-Verlag, New York), pp.269-315(1994). See also WO93 / 16185, and U.S. Patent Nos. 5,571,894 and 5,587,458. See US Pat. No. 5,869,046 for a discussion of Fab and F(ab')2 fragments that contain salvage receptor binding epitope residues and have increased in vivo half-lives.

[0079] Diabodies are antibody fragments that have two antigen binding sites, and can be bivalent or bispecific.See, for example, EP404,097, WO1993 / 01161, Hudson et al.Nat Med.9:129-134(2003), and Hollinger et al.Proc.Natl.Acad.Sci.USA 90:6444-6448(1993).Triabodies and tetrabodies are also described in Hudson et al., Nat.Med.9:129-134(2003).

[0080] A single domain antibody is an antibody fragment that contains all or a portion of the heavy chain variable domain or all or a portion of the light chain variable domain of an antibody. In certain embodiments, a single domain antibody is a human single domain antibody (Domantis, Inc., Waltham, Mass.; see, e.g., U.S. Pat. No. 6,248,516 B1).

[0081] Antibody fragments can be produced by a variety of techniques, including, but not limited to, proteolytic digestion of intact antibodies and production by recombinant host cells (e.g., E. coli or phages), as described herein.

[0082] In some cases, the anti-Tie2 antibodies of the invention provided herein are Fabs.

[0083] In some cases, the Fab binds to Tie2 and may comprise at least one, two, three, four, five, or six CDRs selected from (a) a CDR-H1 comprising the amino acid sequence of any one of SEQ ID NOs: 1-40; (b) a CDR-H2 comprising the amino acid sequence of any one of SEQ ID NOs: 41-80; (c) a CDR-H3 comprising the amino acid sequence of any one of SEQ ID NOs: 81-120; (d) a CDR-L1 comprising the amino acid sequence of any one of SEQ ID NOs: 121-160; (e) a CDR-L2 comprising the amino acid sequence of any one of SEQ ID NOs: 161-200; and (f) a CDR-L3 comprising the amino acid sequence of any one of SEQ ID NOs: 201-240, or a combination of one or more of the above CDRs with one or more variants thereof having at least about 95% sequence identity (e.g., at least 95%, 96%, 97%, 98%, or 99% identity) to any one of SEQ ID NOs: 1-240.

[0084] In some cases, the Fab binds Tie2 and comprises HCDR1-3 and LCDR1-3 comprising the amino acid sequence of SEQ ID NO: as the following antibody clone: TIFF0007638989000003.tif41166TIFF0007638989000004.tif248166

[0085] In some cases, the Fab binds to Tie2 and (a) comprises an amino acid sequence that has at least about 95% sequence identity (e.g., at least 95%, 96%, 97%, 98%, or 99% sequence identity) to, or is 100% identical to, the amino acid sequence of any one of SEQ ID NOs: 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, or 272. and (b) a VL domain comprising an amino acid sequence having at least about 95% sequence identity (e.g., at least 95%, 96%, 97%, 98%, or 99% sequence identity) to, or 100% of, the amino acid sequence of any one of SEQ ID NOs: 242, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, or 273.

[0086] In some instances, the Fab binds Tie2 and comprises a VH domain and a VL domain that comprise amino acid sequences in the following combination: TIFF0007638989000005.tif128166

[0087] In certain embodiments, the antibody provided herein is a chimeric antibody.For certain chimeric antibodies, see, for example, U.S. Patent No. 4,816,567, and Morrison et al., Proc.Natl.Acad.Sci.USA, 81:6851-6855 (1984).In one example, the chimeric antibody comprises a non-human variable region (e.g., a variable domain derived from a non-human primate, such as a mouse, a rat, a hamster, a rabbit, or a monkey) and a human constant domain.In a further example, the chimeric antibody is a "class-switched" antibody whose class or subclass has been changed from that of the parent antibody.The chimeric antibody comprises an antigen-binding fragment thereof.

[0088] In certain embodiments, the chimeric antibody is a humanized antibody. Typically, a non-human antibody is humanized to reduce immunogenicity to humans while retaining the specificity and affinity of the parent non-human antibody. Generally, a humanized antibody comprises one or more variable domains, in which the CDRs, e.g., CDRs (or portions thereof) are derived from a non-human antibody and the FRs (or portions thereof) are derived from a human antibody sequence. Optionally, the humanized antibody will also comprise at least a portion of a human constant region. In some embodiments, some FR residues in the humanized antibody are replaced with corresponding residues from a non-human antibody (e.g., the antibody from which the CDR residues are derived), e.g., to restore or improve antibody specificity or affinity.

[0089] Humanized antibodies and methods for their production are reviewed, for example, in Almagro and Fransson, Front. Biosci. 13:1619-1633 (2008), and further described, for example, in Riechmann et al., Nature 332:323-329 (1988), Queen et al., Proc. Nat'l Acad. Sci. USA 86:10029-10033 (1989), U.S. Patent Nos. 5,821,337, 7,527,791, 6,982,321, and 7,087,409, Kashmiri et al., Methods 36:25-34 (2005) (describing specificity determining region (SDR) grafting), Padlan, Mol. Immunol. 28:489-498 (1991) (describing "resurfacing"), Dall'Acqua et al., Methods 36:43-60 (2005) (describing "FR shuffling"), as well as Osbourn et al., Methods 36:61-68 (2005) and Klimka et al., Br. J. Cancer, 83:252-260 (2000) (describing a "guided selection" approach to FR shuffling).

[0090] Human framework regions that can be used for humanization include framework regions selected using the "best fit" method (see, e.g., Sims et. al., J. Immunol. 151:2296 (1993)), framework regions derived from consensus sequences of human antibodies of particular subgroups of light or heavy chain variable regions (see, e.g., Carter et. al., Proc. Natl. Acad. Sci. USA, 89:4285 (1992), and Presta et. al. J. Immunol., 151:2623 (1993)), human mature (somatically mutated) framework regions or human germline framework regions (see, e.g., Almagro and Fransson, Front. Biosci. 13:1619-1633 (2008)), and framework regions obtained from screening of FR libraries (see, e.g., Baca et al., J. Immunol. 151:2296 (1993)). al., J. Biol. Chem. 272:10678-10684 (1997); and Rosok et al., J. Biol. Chem. 271:22611-22618 (1996).

[0091] In certain embodiments, the antibody provided herein is a human antibody.Human antibodies can be produced using various techniques known in the art.Human antibodies are generally described in van Dijk et al., Curr.Opin.Pharmacol.5:368-74 (2001), and Lonberg, Curr.Opin.Immunol.20:450-459 (2008).

[0092] Human antibodies can be prepared by administering immunogens to transgenic animals that have been modified to produce intact human antibodies or intact antibodies with human variable regions in response to antigen challenge. Such animals typically contain all or part of the human immunoglobulin loci that replace endogenous immunoglobulin loci or are extrachromosomally or randomly integrated into the animal's chromosomes. In such transgenic animals, endogenous immunoglobulin loci are generally inactivated. For a review of methods for obtaining human antibodies from transgenic animals, see Lonberg, Nat.Biotech.23:1117-1125(2005). See, for example, U.S. Patent Nos. 6,075,181 and 6,150,584, which describe the XENOMOUSE™ technology, U.S. Patent No. 5,770,429, which describes the HUANTIBODIES™ technology, U.S. Patent No. 7,041,870, which describes the KM Mouse™ technology, and U.S. Patent Application Publication No. 2007 / 0061900, which describes the VELOCIMOUSE™ technology, and U.S. Patent Nos. 9,809,642 and 9,380,769, which describe the OmniChicken™ technology.) The human variable regions from intact antibodies produced by such animals may be further modified, for example, by combining with different human constant regions.

[0093] Human antibodies can also be produced by hybridoma-based methods. Human myeloma and mouse-human heteromyeloma cell lines for the production of human monoclonal antibodies have been described. (See, for example, Kozbor J.Immunol., 133:3001 (1984); Brodeur et al., Monoclonal Antibody Production Techniques and Applications, pp.51-63 (Marcel Dekker, Inc., New York, 1987); and Boerner et al., J.Immunol., 147:86 (1991).) Human antibodies generated via human B-cell hybridoma technology are also seen in Li et al., Proc.Natl.Acad.Sci.USA, 103:3557-3562 (2006). Additional methods include those described, for example, in U.S. Patent No. 7,189,826 (describing the production of monoclonal human IgM antibodies from hybridoma cell lines) and Ni, Xiandai Mianyixue, 26(4):265-268 (2006) (describing human-human hybridomas). Human hybridoma technology (Trioma technology) is also described in Vollmers and Brandlein, Histology and Histopathology, 20(3):927-937 (2005), and Vollmers and Brandlein, Methods and Findings in Experimental and Clinical Pharmacology, 27(3):185-91 (2005).

[0094] Human antibodies can also be generated by isolating Fv clone variable domain sequences selected from a human-derived phage display library. Such variable domain sequences may then be combined with the desired human constant domains. Techniques for selecting human antibodies from antibody libraries are described below.

[0095] Antibodies of the invention can be isolated by screening combinatorial libraries for antibodies with the desired activity. For example, various methods are known in the art for generating phage display libraries and screening such libraries for antibodies possessing the desired binding characteristics. Such methods are reviewed, for example, in Hoogenboom et al., in Methods in Molecular Biology 178:1-37 (O'Brien et al., ed., Human Press, Totowa, NJ, 2001), and further described, for example, in the McCafferty et al., Nature 348:552-554, Clackson et al., Nature 352:624-628 (1991), Marks et al., J. Mol. Biol. 222:581-597 (1992), Marks and Bradbury, in Methods in Molecular Biology 248:161-175 (Lo, ed., Human Press, Totowa, NJ, 2003), Sidhu et al., J. Mol. Biol. 338(2):299-310 (2004), Lee et. al., J. Mol. Biol. 340(5):1073-1093(2004); Fellouse, Proc. Natl. Acad. Sci. USA 101(34):12467-12472(2004); and Lee et al., J. Immunol. Methods 284(1-2):119-132(2004).

[0096] In a particular phage display method, repertoires of VH and VL genes are cloned separately by polymerase chain reaction (PCR), randomly recombined in a phage library, and then screened for antigen-binding phages as described in Winter et.al., Ann. Rev. Immunol., 12:433-455 (1994). Phages typically display antibody fragments as either single-chain Fv (scFv) fragments or Fab fragments. Libraries from immunized sources provide high affinity antibodies to immunogens without the need for hybridoma construction. Alternatively, natural repertoires can be cloned (e.g., from humans) to provide a single source of antibodies against a wide range of non-self and self antigens without immunization, as described in Griffiths et.al., EMBO J, 12:725-734 (1993). Finally, natural libraries can also be generated synthetically by cloning unrearranged V gene segments from stem cells and using PCR primers containing random sequences to encode highly variable CDR3 regions and achieve rearrangement in vitro, as described in Hoogenboom and Winter, J. Mol. Biol., 227:381-388 (1992). Patent publications describing human antibody phage libraries include, for example, U.S. Pat. No. 5,750,373, and U.S. Patent Publication Nos. 2005 / 0079574, 2005 / 0119455, 2005 / 0266000, 2007 / 0117126, 2007 / 0160598, 2007 / 0237764, 2007 / 0292936, and 2009 / 0002360.

[0097] Antibodies or antibody fragments isolated from a human antibody library are considered herein to be human antibodies or human antibody fragments.

[0098] In certain embodiments, the antibody provided herein is a multispecific antibody, such as, for example, a bispecific antibody, a biparatopic antibody, a trispecific antibody, a tetravalent antibody, a pentavalent antibody, a hexavalent antibody, to name a few. A multispecific antibody is a monoclonal antibody that has binding specificity for at least two different sites. In certain embodiments, a bispecific antibody can bind to two or more different epitopes of Tie2. In certain embodiments, one of the binding specificities is for Tie2, and the other is for any other antigen, such as, for example, VEGF. A bispecific antibody can be prepared as a full-length antibody or an antibody fragment. Any of the anti-Tie2 antibodies described herein can be used to engineer a multispecific antibody.

[0099] In some cases, the multispecific anti-Tie2 antibodies of the invention are bispecific antibodies in which one arm binds Tie2 and the other arm binds VEGF. In other embodiments, such bispecific anti-Tie2 antibodies of the invention also have Fc mutations that abrogate ADCC and / or CDC as described herein. Such bispecific antibodies of the invention comprise heavy and light chains that comprise amino acid sequences in the following combinations: TIFF0007638989000006.tif53166

[0100] In other cases, the multispecific anti-Tie2 antibodies of the present invention are fused to a VEGF extracellular trap protein. In yet other examples, such multispecific anti-Tie2 antibody fusion proteins also have Fc mutations that block ADCC and / or CDC as described herein. Non-limiting examples of such multispecific antibody fusion proteins include the following amino acid sequences: TIFF0007638989000007.tif23166

[0101] Techniques for making multispecific antibodies include, but are not limited to, recombinant coexpression of two immunoglobulin heavy-light chain pairs with different specificities (see Milstein and Cuello, Nature 305:537 (1983)), WO93 / 08829, and Traunecker et al. EMBO, J. 10:3655 (1991)), and "knob-in-hole" engineering (see, e.g., U.S. Pat. No. 5,731,168). Multispecific antibodies can also be produced by manipulating electrostatic steering effects to create antibody Fc-heterodimeric molecules (WO 2009 / 089004 A1), cross-linking two or more antibodies or fragments (see, e.g., U.S. Pat. No. 4,676,980, and Brennan et al., Science, 229:81 (1985)), using leucine zippers to produce bispecific antibodies (see, e.g., Kostelny et al., J. Immunol., 148(5):1547-1553 (1992)), using "diabody" technology to create bispecific antibody fragments (see, e.g., Hollinger et al., Proc. Natl. Acad. Sci. USA, 90:6444-6448 (1993)), using single-chain Fv (sFv) dimers (see, e.g., Gruber et al., J. Immunol., 1999, 11:1112-1114 (1999)), and / or using glycerol-containing glycerols (see, e.g., Gruber et al., J. Immunol., 1999, 11:1112-1114 (1999)). et. al. J. Immunol., 152:5368 (1994)), and by preparing trispecific antibodies, for example as described in Tutt et. al., J. Immunol. 147:60 (1991).

[0102] Engineered antibodies with three or more functional antigen-binding sites, including "octopus antibodies," are also included herein (see, eg, US 2006 / 0025576 A1).

[0103] The antibodies or fragments herein also include "dual acting FAbs" or "DAFs" that contain antigen binding sites that bind to Tie2 and another distinct antigen (see, e.g., US2008 / 0069820).

[0104] In certain embodiments, amino acid sequence variants of the antibodies provided herein (e.g., antibody variants containing one or more amino acid residue modifications) are contemplated. For example, it may be desirable to improve the binding affinity and / or other biological properties of the antibody. Amino acid sequence variants of the antibodies may be prepared by introducing appropriate modifications into the nucleotide sequence encoding the antibody or by peptide synthesis. Such modifications include, for example, deletion of, and / or insertion of, and / or substitution of, residues within the amino acid sequence of the antibody. Any combination of deletion, insertion, and substitution can be made to arrive at the final construct, provided that the final construct possesses the desired characteristics, e.g., antigen binding.

[0105] In certain embodiments, the antibody variant is provided with one or more amino acid substitutions.The target sites for substitution mutagenesis include CDR and FR.Conservative substitution is contemplated, and such substitutions are well known in the art.

[0106] Other amino acid substitutions are described below with reference to amino acid side chain classes. Amino acid substitutions may be introduced into an antibody of interest and the product screened for a desired activity, e.g., retained / improved antigen binding, reduced immunogenicity, or improved or reduced ADCC or CDC. Amino acids may be grouped according to common side chain properties: (1) Hydrophobic: Norleucine, Met, Ala, Val, Leu, lie; (2) Neutral hydrophilic: Cys, Ser, Thr, Asn, Gin; (3) Acidic: Asp, Glu; (4) Basic: His, Lys, Arg; (5) Residues influencing chain orientation: Gly, Pro; (6) Aromatic: Trp, Tyr, Phe.

[0107] Non-conservative substitutions involve exchanging a member of one of these classes for another class.

[0108] One type of substitution variant involves substituting one or more hypervariable region residues and / or FR residues of a parent antibody (e.g., a humanized or human antibody). Generally, the resulting variant selected for further study will have a modification (e.g., an improvement) in a certain biological property compared to the parent antibody (e.g., increased affinity, increased stability, increased expression, altered pI, and / or reduced immunogenicity) and / or will substantially retain a certain biological property of the parent antibody. An exemplary substitution variant is an affinity matured antibody, which can be conveniently generated using, for example, phage display-based affinity maturation techniques such as those described herein. Briefly, one or more CDR residues are mutated, and the mutant antibody is displayed on phage and screened for a certain biological activity (e.g., binding affinity).

[0109] Modifications (e.g., substitutions) can be made in the CDRs, for example, to improve antibody affinity. Such modifications can be made in CDR "hot spots", i.e., residues encoded by codons that undergo frequent mutation during the somatic maturation process (see, e.g., Chowdhury, Methods Mol Biol. 207:179-196 (2008)), and / or residues that contact the antigen, and the resulting variant VH or VL is tested for binding affinity. Affinity maturation by construction and reselection of secondary libraries is described, for example, in Hoogenboom et al., in Methods in Molecular Biology 178:1-37 (O'Brien et al., ed., Human Press, Totowa, NJ, (2001)). In some embodiments of affinity maturation, diversity is introduced into the variable genes selected for maturation by any of a variety of methods (e.g., error-prone PCR, chain shuffling, or oligonucleotide-directed mutagenesis). A secondary library is then generated. The library is then screened to identify any antibody variants with the desired affinity. Another method for introducing diversity involves a CDR-directed approach, in which several CDR residues (e.g., 4-6 residues at a time) are randomized. CDR residues involved in antigen binding can be specifically identified, for example, using alanine scanning mutagenesis or modeling. In particular, CDR-H3 and CDR-L3 are often targeted.

[0110] In certain embodiments, substitutions, insertions, or deletions may occur within one or more CDRs, as long as such modifications do not substantially reduce the antigen-binding ability of the antibody. For example, conservative modifications that do not substantially reduce binding affinity (e.g., conservative substitutions as provided herein) may be made in the CDRs. Such modifications may, for example, be made at residues other than antigen contact residues within the CDRs. In certain embodiments of the variant VH and VL sequences provided above, each CDR is either unmodified or contains no more than one, two, or three amino acid substitutions.

[0111] In certain embodiments, substitutions, insertions, or deletions may be made within one or more FRs, so long as such modifications do not substantially reduce the antigen-binding ability of the antibody. Such modifications may, for example, improve antibody affinity and / or stability (e.g., as assessed by an increase in melting temperature).

[0112] In certain embodiments, one or more amino acid modifications may be introduced into the Fc region of the antibodies provided herein, thereby generating Fc region variants. Fc region variants may include human Fc region sequences (e.g., human IgG1, IgG2, IgG3, or IgG4 Fc regions) that include amino acid residue modifications (e.g., substitutions) at one or more amino acid positions. In certain embodiments, the present invention contemplates antibody variants that retain some, but not all, effector functions, making them desirable candidates for applications in which in vivo antibody half-life is important, but certain effector functions (such as complement and ADCC) are unnecessary or detrimental. In vitro and / or in vivo cytotoxicity assays can be performed to confirm reduced / depleted CDC and / or ADCC activity. For example, Fc receptor (FcR) binding assays can be performed to ensure that the antibody lacks FcγR binding (and thus likely lacks ADCC activity) but retains FcRn binding ability. NK cells, the primary cells for mediating ADCC, express only FcγRIII, whereas monocytes express FcγRI, FcγRII, and FcγRIII. FcR expression on hematopoietic cells is summarized in Table 3 on page 464 of Ravetch and Kinet, Annu. Rev. Immunol. 9:457-492 (1991).

[0113] Non-limiting examples of in vitro assays to assess ADCC activity of a molecule of interest are described in U.S. Pat. No. 5,500,362 (see, e.g., Hellstrom et al., Proc. Nat'l Acad. Sci. USA 83:7059-7063 (1986), and Hellstrom et al., Proc. Nat'l Acad. Sci. USA 82:1499-1502 (1985); U.S. Pat. No. 5,821,337; and Bruggemann et al., J. Exp. Med. 166:1351-1361 (1987)). Alternatively, non-radioactive assay methods may be used (see, e.g., ACTI™ non-radioactive cytotoxicity assay for flow cytometry (CellTechnology, Inc., Mountain View, Calif., and CYTOTOX96™ non-radioactive cytotoxicity assay (Promega, Madison, Wis.)). Useful effector cells for such assays include peripheral blood mononuclear cells (PBMC) and natural killer (NK) cells. Alternatively, or additionally, ADCC activity of the molecule of interest may be measured in vivo, e.g., as described in Clynes et al., Proc. Nat'l Acad. Sci. USA 95:652-656(1998) may be evaluated in animal models such as those disclosed. C1q binding assay may be performed to confirm that the antibody cannot bind C1q and therefore lacks CDC activity. See, for example, the C1q and C3c binding ELISA in WO2006 / 029879 and WO2005 / 100402. To evaluate complement activation, CDC assay may be performed (see, for example, Gazzano-Santoro et al. J.Immunol Methods 202:163(1996), Cragg et al. Blood 101:1045-1052(2003), and Cragg et al. Blood, 103:2738-2743(2004)).FcRn binding and in vivo clearance / half-life determinations can also be performed using methods known in the art (see, e.g., Petkova et al., Int'l. Immunol. 18(12):1759-1769 (2006)).

[0114] The antibodies of the present invention may be engineered with reduced effector function (e.g., reduced complement-dependent cytotoxicity (CDC), antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP)) due to reduced affinity for human FcyRIIIA and / or FcγRIIA and / or FcγRI and / or Clq binding. In some cases, such reduced effector function is achieved by amino acid substitution of one or more of the following Fc region residues according to EU numbering, such as Kabat numbering: N297, L234, L235, D265 and P329. See US6,737,056, US7,332,581 and WO2012 / 130831. In some embodiments, the substitution mutation is one or more of N297G, N297A, L234A, L235A, D265A, and / or P329G. In some embodiments, the substitution mutation is an N297A or N297G substitution mutation. In some embodiments, the substitution mutation comprises the so-called "DANA" Fc mutant with residues D265 and N297 substituted with alanine (U.S. Patent No. 7,332,581). In some embodiments, the substitution mutation comprises the so-called "DANG" mutation with residues substituted as D265A and N297G. In some embodiments, the substitution mutation comprises the "LALA" Fc mutant with residues L234 and L235 substituted with alanine (see Lund, J., et al., (1992) Mol. Immunol., 29, 53-59, and Tamm, A. and Schmidt, RE (1997) Int. Rev. Immunol., 16, 57-85). In other embodiments, the substitution mutation comprises a "LALA-PG" Fc variant in which residues L234 and L235 are replaced with alanine and P329 is replaced with glycine (see Brunker, P., et al. (2016) Mol. Cancer Ther).

[0115] Certain antibody variants have been described with improved or diminished binding to FcRs (see, e.g., U.S. Pat. No. 6,737,056, WO 2004 / 056312, and Shields et.al., J. Biol. Chem. 9(2):6591-6604 (2001)).

[0116] In some embodiments, a substitution mutation is made in the Fc region resulting in a hexamerized antibody as described herein. In some embodiments, such a substitution mutation is E430G. In other embodiments, the E430G mutation may be combined with any of the reduced effector function mutations described above to block CDC and / or ADCC effector function. In one embodiment, the anti-Tie2 antibody of the present invention comprises a heavy chain and a light chain comprising an amino acid sequence having an E430G mutation and L234A, L235A, P329G mutations in the following combinations: TIFF0007638989000008.tif16166

[0117] Antibodies with extended half-lives and improved binding to the neonatal Fc receptor (FcRn), which is involved in the transfer of maternal IgG to the fetus (Guyer et al., J. Immunol. 117:587 (1976) and Kim et al., J. Immunol. 24:249 (1994)), have been described in US2005 / 0014934A1 (Hinton et al.). These antibodies comprise an Fc region with one or more substitutions that improve binding of the Fc region to FcRn. Such Fc variants include those having substitutions at one or more of the following Fc region residues: 238, 256, 265, 272, 286, 303, 305, 307, 311, 312, 317, 340, 356, 360, 362, 376, 378, 380, 382, ​​413, 424, or 434, such as a substitution at Fc region residue 434 (U.S. Patent No. 7,371,826). See also Duncan & Winter, Nature 322:738-40 (1988), U.S. Patent No. 5,648,260, U.S. Patent No. 5,624,821, and WO 94 / 29351 for other examples of Fc region variants.

[0118] The present invention also provides immunoconjugates comprising an anti-Tie2 antibody herein conjugated to one or more cytotoxic agents, such as a chemotherapeutic agent or drug, a growth inhibitor, a toxin (e.g., a protein toxin, an enzymatically active toxin of bacterial, fungal, plant, or animal origin, or a fragment thereof), or a radioactive isotope.

[0119] In one embodiment, the immunoconjugate is an immunoconjugate in which the antibody is coupled to a maytansinoid (see U.S. Pat. Nos. 5,208,020, 5,416,064, and European Patent No. 0425235B1), an auristatin such as the monomethyl auristatin drug moieties DE and DF (MMAE and MMAF) (see U.S. Pat. Nos. 5,635,483, 5,780,588, and 7,498,298), a dolastatin, a calicheamicin or a derivative thereof (see U.S. Pat. Nos. 5,712,374, 5,714,586, 5,739,116, 5,767,285, 5,770,701, 5,770,710, 5,773,001, and 5,877,296; Hinman et al., J. Med. Soc. 2002; 2003; 2004; 2005; 2006; 2007; 2009; 2010; et al., Cancer Res. 53:3336-3342 (1993), and Lode et al., Cancer Res. 58:2925-2928 (1998)), anthracyclines such as daunomycin or doxorubicin (Kratz et al., Current Med. Chem. 13:477-523 (2006), Jeffrey et al., Bioorganic & Med. Chem. Letters 16:358-362 (2006), Torgov et al., Bioconj. Chem. 16:717-721 (2005), Nagy et al. Proc. Natl. Acad. Sci. USA 97:829-834 (2000), Dubowchik et al., Bioorg. & Med. Chem. Letters 12:1529-1532 (2002), King et al., J. Med. Chem. 45:4336-4343 (2002), and U.S. Patent No. 6,630,579), methotrexate, vindesine, taxanes such as docetaxel, paclitaxel, larotaxel, tesetaxel, and ortataxel, trichothecenes, and CC1065.

[0120] In another embodiment, the immunoconjugate comprises an antibody as described herein conjugated to an enzymatically active toxin or fragment thereof, including, but not limited to, diphtheria A chain, a nonbinding active fragment of diphtheria toxin, exotoxin A chain (from Pseudomonas aeruginosa), ricin A chain, abrin A chain, modeccin A chain, alpha-sarcin, Aleurites fordii protein, dianthin protein, Phytolaca americana proteins (PAPI, PAPII, and PAP-S), Momordica charantia inhibitor, curcin, crotin, Sapaonaria officinalis inhibitor, gelonin, mitogenin, restrictocin, phenomycin, enomycin, and the trichothecenes.

[0121] In another embodiment, the immunoconjugate comprises an antibody as described herein conjugated to a radioactive atom to form a radioconjugate. A variety of radioisotopes are available for the production of radioconjugates. For example, At 211 , I 131 , I 125 , Y 90 , Re 186 , Re 188 , Sm 153 , Bi 212 , P 32 , Pb 212 , and radioisotopes of Lu. When used for detection, the radioconjugate may contain a radioactive atom (e.g., Tc99m or 1123) for scintigraphy or a spin label (again, iodine-123, iodine-131, indium-111, fluorine-19, carbon-13, nitrogen-15, oxygen-17, gadolinium, manganese or iron, etc.) for nuclear magnetic resonance (NMR) imaging (also known as magnetic resonance imaging, MRI).

[0122] In another embodiment, the immunoconjugate comprises an antibody described herein conjugated to a non-cytotoxic agent, e.g., an artemisinin such as artusenate, or a cannabinoid, or naltrexone, or aspirin, or a statin, or a metabolic agent such as metformin, doxycycline, and an antiparasitic agent.

[0123] Conjugates of antibodies and cytotoxic or non-cytotoxic agents may be made using a variety of bifunctional protein coupling agents, such as N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP), succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), iminothiolane (IT), bifunctional derivatives of imidoesters (such as dimethyl adipimidate, HCL), active esters (such as disuccinimidyl suberate), aldehydes (such as glutaraldehyde), bis-azido compounds (such as bis(p-azidobenzoyl)hexanediamine), bis-diazonium derivatives (bis-(p-diazoniumbenzoyl)-ethylenediamine), diisocyanates (such as toluene 2,6-isocyanate), and bis-active fluorine compounds (such as 1,5-difluoro-2,4-dinitrobenzene). For example, see Vitetta et.al. Science, 238:1098 (1987) for ricin immunotoxins. Carbon-14 labeled 1-isothiocyanatobenzyl-3-methyldiethylenetriaminepentaacetic acid (MX-DTPA) is an exemplary chelating agent for conjugating radionucleotides to antibodies. See WO94 / 11026. The linker can be a "cleavable linker" that facilitates the release of the cytotoxic drug inside the cell. For example, an acid-labile linker, a peptidase-sensitive linker, a photolabile linker, a dimethyl linker, or a disulfide-containing linker can be used (Chari et.al., Cancer Res. 52:127-131 (1992), U.S. Patent No. 5,208,020).

[0124] The immunoconjugates or ADCs herein expressly contemplate, but are not limited to, those conjugates prepared with cross-linking reagents, including, but not limited to, BMPS, EMCS, GMBS, HBVS, LC-SMCC, MBS, MPBH, SBAP, SIA, STAB, SMCC, SMPB, SMPH, sulfo-EMCS, sulfo-GMBS, sulfo-KMUS, sulfo-MBS, sulfo-SIAB, sulfo-SMCC, and sulfo-SMPB, and SVSB (succinimidyl-(4-vinylsulfone)benzoate), which is commercially available (e.g., from Pierce Biotechnology, Inc., Rockford, Ill., USA).

[0125] In one aspect, the anti-Tie2 antibody of the present invention is useful for detecting the presence of Tie2 in biological samples.As used herein, the term "detection" includes quantitative or qualitative detection.In certain embodiments, biological samples include cells or tissues.In certain embodiments, such tissues include normal tissues and / or cancer tissues that express Tie2 at higher levels compared to other tissues.

[0126] In one embodiment, an anti-Tie2 antibody is provided for use in a method of diagnosis or detection. In a further aspect, a method is provided for detecting the presence of Tie2 in a biological sample. In certain embodiments, the method comprises contacting a biological sample with an anti-Tie2 antibody described herein under conditions that allow the binding of the anti-Tie2 antibody to Tie2, and detecting whether a complex is formed between the anti-Tie2 antibody and Tie2. Such a method may be an in vitro or in vivo method. In one embodiment, the anti-Tie2 antibody is used to select a subject for therapy with an anti-Tie2 antibody, for example, Tie2 is a biomarker for patient selection.

[0127] Any of the antibodies (e.g., anti-Tie2 antibodies) described herein may be produced using recombinant methods and compositions, e.g., as described in U.S. Pat. No. 4,816,567. In one embodiment, an isolated nucleic acid is provided that encodes an anti-Tie2 antibody described herein. Such a nucleic acid may encode an amino acid sequence comprising the VL and / or an amino acid sequence comprising the VH of the antibody (e.g., the light and / or heavy chains of the antibody). In a further embodiment, one or more vectors (e.g., expression vectors) comprising such a nucleic acid are provided. In a further embodiment, a host cell comprising such a nucleic acid is provided. In one such embodiment, the host cell comprises (e.g., is transformed with) (1) a vector comprising a nucleic acid encoding an amino acid sequence comprising the VL of the antibody and an amino acid sequence comprising the VH of the antibody, or (2) a first vector comprising a nucleic acid encoding an amino acid sequence comprising the VL of the antibody and a second vector encoding an amino acid sequence comprising the VH of the antibody. In one embodiment, the host cell is a eukaryote, e.g., a Chinese hamster ovary (CHO) cell or a lymphoid cell (e.g., a YO, NSO, Sp20 cell). In one embodiment, a method of making an anti-Tie2 antibody is provided, the method comprising culturing a host cell comprising nucleic acid encoding the antibody as provided above under conditions suitable for expression of the antibody, and optionally recovering the antibody from the host cell (or host cell culture medium).

[0128] For recombinant production of an anti-Tie2 antibody, nucleic acid encoding the antibody is isolated and inserted into one or more vectors for further cloning and / or expression in a host cell, e.g., as described above. Such nucleic acid can be readily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes capable of specifically binding to genes encoding the heavy and light chains of the antibody).

[0129] Suitable host cells for cloning or expressing antibody-encoding vectors include prokaryotic or eukaryotic cells as described herein. For example, antibodies can be produced in bacteria, particularly when glycosylation and Fc effector functions are not required. For the expression of antibody fragments and polypeptides in bacteria, see, for example, U.S. Patent Nos. 5,648,237, 5,789,199, and 5,840,523. Also see Charlton, Methods in Molecular Biology, Vol. 248 (BKC Lo, ed., Humana Press, Totowa, NJ, 2003), pp. 245-254, which describes the expression of antibody fragments in E. coli. After expression, antibodies can be isolated from bacterial cell paste in a soluble fraction and can be further purified.

[0130] In addition to prokaryotes, eukaryotic microbes such as filamentous fungi or yeast are suitable cloning or expression hosts for antibody-encoding vectors, including fungal and yeast strains in which the glycosylation pathway has been "humanized," resulting in the production of antibodies with partial or fully human glycosylation patterns. See Gerngross, Nat. Biotech. 22:1409-1414 (2004), and Li et al., Nat. Biotech. 24:210-215 (2006).

[0131] Suitable host cells for the expression of glycosylated antibodies are also derived from multicellular organisms (invertebrates and vertebrates). Examples of invertebrate cells include plant cells and insect cells. Many baculovirus strains have been identified that can be used with insect cells, particularly for transfection into Spodoptera frugiperda cells.

[0132] Plant cell cultures can also be used as hosts. See, for example, U.S. Patent Nos. 5,959,177, 6,040,498, 6,420,548, 7,125,978, and 6,417,429 (which describe the PLANTIBODIES™ technology for producing antibodies in transgenic plants).

[0133] Vertebrate cells may also be used as hosts. For example, mammalian cell lines adapted to grow in suspension may be useful. Other examples of useful mammalian host cell lines are monkey kidney CV1 line transformed with SV40 (COS-7), human embryonic kidney lines (e.g., 293 or 293 cells as described in Graham et.al. J. Gen. Virol. 36:59 (1977)), baby hamster kidney cells (BHK), mouse Sertoli cells (e.g., T cells as described in Mather, Biol. Reprod. 23:243-251 (1980)), and the like. M4 cells), monkey kidney cells (CV1); African green monkey kidney cells (VERO-76), human cervical carcinoma cells (HELA), dog kidney cells (MDCK), buffalo rat liver cells (BRL 3A), human lung cells (W138), human liver cells (Hep G2); mouse mammary tumor (MMT 060562), e.g., TRI cells, MRC 5 cells, and FS4 cells as described in Mather et.al., Annals NYAcad.Sci.383:44-68(1982). Other useful mammalian host cell lines include Chinese hamster ovary (CHO) cells, including DHFR-CHO cells (Urlaub et.al., Proc. Natl. Acad.Sci.USA 77:4216(1980)), and myeloma cell lines such as YO, NSO, and Sp2 / 0. For a review of certain mammalian host cell lines suitable for antibody production, see, e.g., Yazaki and Wu, Methods in Molecular Biology, Vol. 248 (BKC Lo, ed. Humana Press, Totowa, NJ), pp. 255-268 (2003).

[0134] The anti-Tie2 antibodies provided herein can be identified, screened or characterized for their physical / chemical properties and / or biological activity by various assays that are known in the art and described in the Examples and throughout the specification.

[0135] In one embodiment, the antibodies of the invention are tested for their antigen binding activity by known methods, such as, for example, ELISA, Western blot, surface plasmon resonance assays (eg, BIACORE™).

[0136] In one aspect, antigen binding activity (e.g., as indicated by KD) is measured using a BIACORE™ surface plasmon resonance (SPR) assay. For example, an assay using a BIACORE™-2000 or BIACORE™-3000 (BIAcore, Inc., Piscataway, NJ) is performed at 25° C. with an antigen CM5 chip immobilized at about 10 response units (RU). In one embodiment, a carboxymethylated dextran biosensor chip (CM5, BIAcore, Inc.) is activated with N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) according to the supplier's instructions. The antigen is diluted to 5 μg / ml (about 0.2 μM) with 10 mM sodium acetate, pH 4.8, and then injected at a flow rate of 5 μl / min to achieve about 10 response units (RU) of the coupling protein. After antigen injection, 1 M ethanolamine is injected to block unreacted groups. For kinetic measurements, two-fold serial dilutions of Fab (0.78 nM to 500 nM) are injected in PBS containing 0.05% polysorbate 20 (TWEEN™-20) surfactant (PBST) at a flow rate of approximately 25 μl / min at 25° C. The association rate (k on ) and dissociation rate (k off) is calculated using a simple one-to-one Langmuir binding model (BIACORE™ Evaluation Software version 3.2) by simultaneously fitting the association and dissociation sensorgrams. The equilibrium dissociation constant (KD) is calculated as k off / k on The on-rate is calculated as the ratio of 10 to 10. See, for example, Chen et al., J. Mol. Biol. 293:865-881 (1999). 6 M -1 s -1 If the on-rate exceeds , the on-rate can be determined using a fluorescence quenching technique that measures the increase or decrease in fluorescence emission intensity (excitation = 295 nm, emission = 340 nm, 16 nm bandpass) of 20 nM antibody (Fab form) in PBS (pH 7.2) at 25 °C in the presence of increasing concentrations of antigen as measured with a spectrometer such as a stopped-flow equipped spectrometer with a stirred cuvette (Aviv Instruments) or an 8000 series SLM-AMINCO™ spectrophotometer (ThermoSpectronic). The KD may be measured using a BIACORE™ SPR assay.

[0137] In another aspect, a competition assay can be used to identify antibodies that compete with an antibody as described herein for binding to Tie2. In certain embodiments, such a competing antibody binds to the same epitope (e.g., a linear or conformational epitope) bound by an antibody as described herein. Detailed exemplary methods for mapping the epitope to which an antibody binds are provided in Morris (1996) "Epitope Mapping Protocols," in Methods in Molecular Biology vol. 66 (Humana Press, Totowa, NJ).

[0138] In an exemplary competitive assay, immobilized Tie2 is incubated in a solution containing a first labeled antibody that binds to Tie2 and a second unlabeled antibody that is being tested for its ability to compete with the first antibody for binding to Tie2. The second antibody may be present in a hybridoma supernatant. As a control, immobilized Tie2 is incubated in a solution containing the first labeled antibody but not the second unlabeled antibody. After incubation under conditions that allow the binding of the first antibody to Tie2, excess unbound antibody is removed and the amount of label associated with immobilized Tie2 is measured. If the amount of label associated with immobilized Tie2 is substantially reduced in the test sample compared to the control sample, it indicates that the second antibody competes with the first antibody for binding to Tie2. See Harlow and Lane (1988) Antibodies: A Laboratory Manual ch.14 (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY).

[0139] In one embodiment, an assay is provided for identifying the anti-Tie2 antibody having biological activity.See the Examples section.Biological activity can include, for example, activating, agonizing, increasing, enhancing one or more biological activities of Tie2.Antibodies having such biological activity in vivo and / or in vitro are also provided.

[0140] In certain embodiments, the antibodies of the present invention are tested for such biological activity. In certain embodiments, the anti-Tie2 antibodies bind to Tie2 and reduce or inhibit its serine protease activity against one or more Tie2 substrates, including, for example, H2-Opt substrate, α-casein, β-casein, or BODIPY™ FL casein substrate, or any other suitable Tie2 substrate known in the art. In certain embodiments, the anti-Tie2 antibodies inhibit Tie2 activity with an IC50 of 50nM, 30nM, 25nM, 20nM, 15nM, 10nM, 5nM, 3nM, 2.5nM, 2nM, 1nM, 800pM, 600pM, 500pM, 400pM, 300pM, 200pM, 100pM, 50pM, or less against one or more Tie2 substrates.

[0141] In certain embodiments, labeled anti-Tie2 antibodies are provided. Labels include, but are not limited to, labels or moieties that are directly detected (e.g., fluorescent labels, chromophore labels, electron-dense labels, chemiluminescent labels, and radioactive labels), as well as moieties, such as enzymes or ligands, that are indirectly detected, for example, through enzymatic reactions or molecular interactions. Exemplary labels include radioisotopes, 32 P, 14 C. 125 I, 3 H, and 131I, fluorophores such as rare earth chelates or fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, luceriferases such as firefly luciferase and bacterial luciferase (U.S. Pat. No. 4,737,456), luciferin, 2,3-dihydrophthalazinedione, horseradish peroxidase (HRP), alkaline phosphatase, β-galactosidase, glucoamylase, lysozyme, saccharide oxidases such as glucose oxidase, galactose oxidase and glucose-6-phosphate dehydrogenase, heterocyclic oxidases such as uricase and xanthine oxidase (coupled with an enzyme that uses hydrogen peroxide to oxidize a dye precursor, such as HRP, lactoperoxidase or microperoxidase), biotin / avidin, spin labels, bacteriophage labels, stable free radicals, and the like. In another embodiment of the invention, the antibody need not be labeled and the presence of the antibody can be detected using a labeled antibody that binds to the antibody, such as, for example, an anti-horseradish peroxidase antibody, which is well known in the art.

[0142] The antibodies of the present invention may be used in any known assay method, such as competitive binding assays, direct and indirect sandwich assays, and immunoprecipitation assays. See Zola et al., Monoclonal Antibodies: A Manual of Techniques, pp. 147-158 (CRC Press, Inc. 1987).

[0143] Competitive binding assays rely on the ability of a labeled standard to compete with the test sample analyte for binding to a limited amount of antibody. The amount of antigen in the test sample is inversely proportional to the amount of standard that becomes bound to the antibody. To facilitate determining the amount of standard that becomes bound, the antibody is generally insolubilized before or after the competition, so that the standard and analyte that are bound to the antibody can be conveniently separated from the standard and analyte that remain unbound.

[0144] Sandwich assays involve the use of two antibodies, each capable of binding to a different immunogenic portion or epitope of the protein to be detected. In a sandwich assay, the test sample analyte is bound by a first antibody immobilized on a solid support, and then a second antibody binds to the analyte, thus forming an insoluble ternary complex. See, for example, U.S. Pat. No. 4,376,110. The second antibody itself may be labeled with a detectable moiety (direct sandwich assay) or may be measured using an anti-immunoglobulin antibody that is labeled with a detectable moiety (indirect sandwich assay). For example, one type of sandwich assay is the ELISA assay, in which the detectable moiety is an enzyme.

[0145] For immunohistochemistry, samples may be fresh or frozen, or may be embedded in paraffin and fixed with a preservative such as formalin.

[0146] Pharmaceutical preparations Therapeutic formulations of the fusion polypeptides of the invention, including the anti-Tie2 antibodies or antibody fragments or variants thereof, or the immunoconjugates of the invention, or combinations thereof with anti-VEGF antibodies or recombinant VEGF fusion proteins as provided herein, can be prepared for storage as lyophilized formulations or aqueous solutions by mixing the polypeptides with the desired purity with optional "pharmaceutical acceptable" carriers, excipients, or stabilizers (all of which are referred to as "excipients"). For example, buffers, stabilizers, preservatives, isotonicity agents, non-ionic surfactants, antioxidants, and various other additives. See, for example, Remington's Pharmaceutical Sciences, 16 th edition, A. Osol, Ed. (1980). Such additives must be nontoxic to recipients at the dosages and concentrations employed.

[0147] Therapeutic Methods and Compositions The fusion polypeptides of the invention, including the anti-Tie2 antibodies and antibody fragments of the invention, or the immunoconjugates of the invention, or combinations thereof with anti-VEGF antibodies or recombinant VEGF fusion proteins as provided herein, may be used in therapeutic methods for treating, preventing, and / or alleviating a variety of diseases, including any of the Tie2 dysregulation diseases defined herein. EXAMPLES

[0148] The following are examples of methods and compositions of the present invention: Given the general description above, it will be understood that other various embodiments may be practiced.

[0149] Example 1: Immunization of transgenic chickens to isolate fully human anti-Tie2 antibodies Because the extracellular domains (ECDs) of human and chicken Tie2 orthologues are only 62% identical, in contrast to the 90% identity observed between mouse and human Tie2 orthologues, using chickens to generate Tie2 antibodies would greatly expand the epitope range that the antibodies can access.

[0150] The antibodies were generated by immunizing a proprietary transgenic chicken, OmniChicken® (Crystal Biosciences, Inc, Emeryville, Calif.), with recombinantly produced and purified ECD of human Tie2. Through deletion of endogenous immunoglobulin-encoding genes from the chicken genome and their replacement with human immunoglobulin-encoding genes, OmniChicken can be "humanized" to produce fully human antibodies (see U.S. Pat. Nos. 9,809,642 and 9,380,769).

[0151] Next, a high-throughput single B cell screening and cloning approach, Gel Encapsulated Microenvironment (GEM) technology (Crystal Bioscience, Inc, Emeryville, CA), was used to rapidly screen millions of B cells for antibodies that specifically bind to Tie2 (see US Patents 8,415,173 and 8,030,095). GEM involves colocalizing single antibody-secreting B lymphocytes from immunized animals within gel microdroplets containing one or more particulate reporters (Mettler Izquierdo et.al. 2016). The reporters used were polystyrene beads coated with Tie2 antigen (ECD of human or mouse Tie2 recombinant protein) and / or cells expressing human Tie2. When Tie2 antibody-producing B cells were incorporated into GEM, binding to antigen-coated beads or Tie2-expressing cells was detected using a red fluorescent secondary antibody. Through the use of single B cell cloning technology, many appropriately paired heavy and light chain variable domains were found. These sequences were cloned into antibody expression vectors. A total of 236 recombinant antibodies were expressed via transient transfection to confirm specificity and evaluate functional activity in downstream assays.

[0152] Example 2: Primary antibody screening All 236 antibodies generated as described above in Example 1 were tested for binding to recombinant human Tie2 ECD protein using an ELISA method. The majority of the screened antibodies were found to be strong binders by ELISA, many with EC50 values ​​in the low pM range.

[0153] Cross-reactivity to mouse Tie2 by binding to recombinant mouse Tie2 ECD protein was used as a primary screen. These binding studies demonstrated that approximately 90% of the anti-Tie2 antibodies generated were cross-reactive between mouse and human Tie2 proteins, indicating that they may be suitable for in vivo testing in established animal models.

[0154] Next, we performed a screen for recognition and binding to human full-length Tie2 expressed in its native conformation on the plasma membrane of live cells. For these experiments, human umbilical vein endothelial cells (HUVECs) were obtained from donors. Using flow cytometry, we also demonstrated that approximately 70% of the antibodies that bound to human Tie2 ECD protein by ELISA also bound to native Tie2 expressed on cells [data not shown].

[0155] Example 3: Agonistic antibodies identified by functional screening All Tie2 antibodies that bound to HUVEC cells as described in Example 2 above were then tested for agonistic properties using a homogenous immunoassay (AlphaLISA™) screening platform designed to detect antibody-induced intracellular levels of phosphorylated (activated) ERK (pERK or p42 / p44) and phosphorylated (active) Akt (pAkt), both of which are known downstream signaling effectors of Tie2.

[0156] In this screening method, HUVEC cells were plated in 96-well plates, serum-starved for 3 hours, and then treated with Ang1 (positive control), hIgG1 (nonspecific human IgG1 negative control antibody) or anti-Tie2 antibodies at a concentration of 180 nM. After 20 minutes of incubation, cells were lysed and lysates were analyzed by a specific AlphaLISA assay for the presence of pERK and total ERK, as well as pAkt and total Akt. To be designated as an "active agonist anti-Tie2 antibody," an antibody clone was required to induce Tie2 signaling, as determined by increased levels of intracellular pAkt and pERK (normalized to total Akt and total ERK, respectively). Antibodies that were able to induce increased levels of pERK and pAkt were ranked based on their activity compared to the levels of pERK and pAkt induced by Ang1 treatment. Antibodies capable of increasing pERK and pAkt levels by 75% or more over those seen after Ang1 treatment were considered comparable to Ang1 and were carried forward for further testing.

[0157] After identifying hits in the primary functional screen, the variable domains from the most active anti-Tie2 antibodies were reformatted onto a different human IgG backbone, more suitable for therapeutic use. Specifically, the variable domains of all lead candidates were cloned into an expression plasmid containing an Fc domain from human IgG1, including an asparagine to alanine mutation at amino acid position 297 (N297A). The Tie2 agonist activity of these reformatted antibodies was confirmed by Western blot analysis for antibody-induced increases in phospho-Tie2 (pTie2) (Figure 1A), and pERK and pAkt (Figures 1B and 1C) as previously described.

[0158] Thus, the anti-Tie2 antibodies of the present invention were found to activate both proximal and distal Tie2 signaling events and were pursued for further investigation.

[0159] Example 4: Determining antibody potency in vitro To enable ranking of the anti-Tie2 antibodies of the invention based on potency, a highly sensitive assay for determining antibody EC50 values ​​was developed.

[0160] In this assay, HUVEC cells were starved for 3 hours and then treated with increasing concentrations of Ang1, anti-Tie2 antibody, or negative control hIgG1 for 20 minutes before cell lysis. Cell lysates were then subjected to Western blotting and quantitative fluorescent imaging to determine the levels of pAkt relative to total Akt and analyzed as follows to determine the respective EC50 values: Ang1=0.54nM, Ab #1=0.91nM, Ab #2=0.48nM, Ab #3=0.45nM, and WT=1.33nM. See Figure 2.

[0161] Analysis of EC50 values ​​revealed that most antibodies exhibited potent Tie2 activation at subnanomolar concentrations comparable to the potency of Ang1 in the same assay. Taken together, the functional assay utilized identified anti-Tie2 antibodies with the ability to induce Tie2 signaling to levels comparable to those seen with Ang1.

[0162] Example 5: Anti-Tie2 antibodies reduce fluid leakage in an in vitro model of the endothelial barrier To investigate the potential physiological effects of anti-Tie2 antibodies on cellular ability to control permeability, a simplified in vitro model of the endothelial barrier was established. This model allowed for further characterization of antibodies based on their ability to enhance and / or protect physiological permeability induced by increased levels of VEGF in the microenvironment. See Figure 3 for a schematic of the model setup.

[0163] Intact confluent monolayers of HUVEC cells were cultured on semipermeable membranes to form adherent structures with tight junctions. Cells were treated with 100 ng / ml VEGF with or without Ang1 or anti-Tie2 antibodies. Permeability was assayed at different time points over 6 hours by measuring the amount of fluorescein-conjugated dextran that penetrated through the cell monolayer into the receiver well beneath the membrane. Leakage rate was measured over time as the number of fluorescein units accumulated in the receiving well. The difference in endothelial barrier leakage between PBS (negative control) and Ang1 (positive control) was determined. The ability of anti-Tie2 antibodies to reduce endothelial barrier leakage was normalized to the effect Ang1 treatment in the same assay (% Ang1 activity). See Figure 4.

[0164] Statistical analysis revealed that anti-Tie2 antibodies significantly reduced VEGF-induced fluid leakage through this matrix of primary endothelial cells, whereas the human IgG1 negative control did not. Thus, anti-Tie2 antibodies were both able to stimulate downstream Tie2 signaling in biochemical assays and were also able to reduce leakage in orthogonal in vitro physiological assays.

[0165] Example 6: Anti-Tie2 agonist antibodies activate Tie2 in the presence of high levels of Ang2 Patients suffering from diabetic macular edema (DME) show a marked increase in systemic and intravitreal levels of Ang2 (Loukovaara et.al.2013b, Regula et.al.2017). Considering the possibility that high levels of Ang2 may interfere with anti-Tie2 antibody activity by sharing a similar binding site on Tie2, or whether antibody binding is influenced by an allosteric effect of Ang2 on the Tie2 structure, we determined the functional properties of anti-Tie2 antibodies in the presence of saturating concentrations of Ang2. Ang2 functions as a weak Tie2 agonist capable of inducing signaling in in vitro assays in the absence of Ang1, but at lower levels than can be achieved by Ang1 (Yuan et al.2009). The in vitro experiments described below corroborated these findings.

[0166] To determine the saturation level of Ang2 during Tie2 signaling in an in vitro model system, HUVEC cells were starved for 3 hours and treated with increasing concentrations of Ang2, followed by lysis and analysis of pERK / Erk levels by Western blotting. See Figure 4A. Blots were analyzed using quantitative fluorescent imaging and normalized levels of pERK were plotted as a function of Ang2 concentration. The minimal concentration of Ang2 that saturated Tie2 signaling was determined to be 2.5ug / mL. See Figure 5B. In subsequent experiments, HUVEC cells were co-treated with Ang2 at a concentration of 2.5ug / mL (43nM) and anti-Tie2 antibody at a concentration of 12nM for 20 minutes prior to cell lysis and analysis of pErk / Erk (Figures 5C and 5D) and pAkt / Akt (Figures 5E and 5F) levels.

[0167] The results of these experiments demonstrated that anti-Tie2 antibodies were potent Tie2 pathway activators, even in the presence of high Ang2 concentrations. Surprisingly, Tie2 activity in cells exposed to both anti-Tie2 antibodies and Ang2 was higher than that seen with either treatment alone.

[0168] Example 7: Anti-Tie2 antibodies utilize a non-ligand competitive binding mechanism To further investigate the interaction of anti-Tie2 antibodies with angiopoietins at the Tie2 receptor level, a competitive binding assay was established using recombinant proteins and biolayer interferometry technology (BLI), a label-free technique for measuring biomolecular interactions.

[0169] In these experiments, His-tagged recombinant Tie2 ECD was captured on a Ni-NTA biosensor and subsequently exposed sequentially to a specific anti-Tie2 antibody, then Ang1 or Ang2 to assess potential binding competition. In these experiments, Ang1 and Ang2 were able to bind to Tie2 receptors precomplexed with anti-Tie2 antibodies. See Figures 6A-B. Thus, the anti-Tie2 agonist antibodies of the present invention were found to bind to Tie2 in a non-Ang1 and Ang2 ligand competitive manner and enhance Tie2 signaling in the presence of Ang2 to levels higher than the anti-Tie2 antibody alone.

[0170] Taken together with the Tie2 pathway signaling results described above, the data characterizing the binding mechanism of the anti-Tie2 antibodies of the invention indicate a mechanism of action that is completely independent of angiopoietin levels.

[0171] Example 8: Anti-Tie2 agonist antibodies are cross-reactive to Tie2 variants expressed by other animal species Genetically engineered cell lines overexpressing mouse, rat, rabbit, pig, and cynomolgus monkey Tie2 orthologues were used to investigate the cross-reactivity profile of anti-Tie2 antibodies to Tie2 orthologues expressed by preclinical animal model species.

[0172] Briefly, expression plasmids containing various full-length Tie2 coding sequences were used to transfect primary human endothelial cells (HUVEC) as well as human embryonic kidney (HEK293) cells. Stable cell populations were selected by treating transfectants with puromycin antibiotics for 2 weeks, and single cell clones were generated using fluorescence-activated cell sorting. Upon confirmation of cell surface expression of these Tie2 variants, the cell lines were used to determine the apparent Kd (EC50) values ​​of anti-Tie2 antibodies on various Tie2 proteins. To obtain this data, the cross-reactivity profile of the anti-Tie2 antibodies of the present invention was determined. Cells were labeled with decreasing concentrations of anti-Tie2 antibodies #1, #2, and #3 (10, 5, 2.5, 1.25, 0.63, 0.31, and 0.16ug / mL) or non-specific hIgG1 antibody (10ug / mL), followed by rabbit anti-human IgG antibody conjugated to Alexa Fluor 488 fluorophore (20ug / mL). EC50 values ​​were generated using ForeCyt™ software. See Tables 1 and 2.

[0173] [Table 1]

[0174] [Table 2]

[0175] These experiments demonstrate cross-reactive binding of anti-Tie2 antibodies across human and preclinical species.

[0176] Example 9: Anti-Tie2 antibodies recognize distinct epitopes on human Tie2 To understand the diversity of epitope coverage of the Tie2 antibody panel, anti-Tie2 antibodies were evaluated for cross-competition using BLI technology.

[0177] Recombinant human Tie2 ECD is loaded onto the biosensor and then exposed to anti-Tie2 antibodies (M1 = mouse anti-Tie2 mAb, Ab #1, Ab #3, M2 = mouse anti-Tie2, Ab #2, WT) to assess initial binding. The probe is then exposed to a second anti-Tie2 antibody to assess a second binding event. A positive wavelength shift indicates that the second antibody can bind to the previously formed Tie2-antibody complex, indicating that these antibodies bind different epitopes and do not compete for binding to the Tie2 protein. Positive wavelength shifts indicating co-binding of two anti-Tie2 antibodies are shown in gray and light gray, and the shift values ​​(nm) are embedded in each cell. As expected, antibodies that were previously bound to Tie2 blocked binding of the same antibodies to the complex (black shaded areas). See Figure 7.

[0178] These experiments demonstrated that across a panel of six anti-Tie2 agonist antibodies, there was no cross-competition for binding to Tie2, indicating that each candidate recognized a different epitope on the receptor.

[0179] Example 10: Oxygen-induced retinopathy (OIR) mouse model In this study, 40 C57BL / 6J pups were housed (n=10 per cage) on postnatal day 7 (P7) in a hyperoxic chamber (75% O2) for 5 days, resulting in vascular regression of the central retina. CD-1 foster mothers were replaced before and 2–3 days after entering the chamber. At P12, pups were returned to room air, where relative hypoxia induces abnormal angiogenesis. Endotoxin-free 1x PBS vehicle, 10 mg / kg, HuIgG isotype control, or 10 mg / kg anti-Tie2 clone #3 were then administered intraperitoneally. At P17, all groups, including naïve OIR mice, were euthanized. Eyes were removed and fixed in 4% paraformaldehyde for 1 h.

[0180] Retinas were dissected and incubated overnight in 1 mM CaCl2 in PBS with rhodamine-labeled lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) (1:100) to visualize areas of voided (VO) or neovascularized (NV). Stained retinas were flat-mounted onto slides and imaged on a Zeiss® AxioScan. Images were analyzed on a Visiopharm® to determine %VO or %NV of the whole retina.

[0181] As shown in Figure 8B, anti-Tie2 mAb, but not isotype control mAb or vehicle, was found to have a significant positive effect on areas of lost blood vessels (i.e., avascular), but did not appear to reduce NV (Figure 8A). Promotion of healthy vasculature regrowth may be beneficial to alleviate adverse outcomes of chronic anti-VEGF therapy, such as geographic atrophy or missing capillaries in patients with ocular disease. See Kim J, et al., Science Advances, Vol. 5 Feb 13 (2019); M. Young, et al., Retina 34, 1308-1315 (2014); T. Kurihara, et al., J. Clin. Invest. 122, 4213-4217 (2012).

[0182] Example 11: Laser-induced choroidal neovascularization (CNV) mouse model Male C57BL / 6J mice (6-8 weeks) were anesthetized with ketamine / xylazine cocktail before laser procedure. CNV lesions were induced by laser photocoagulation using a diode laser (IRIDEX®, Oculight® GL) and slit lamp (Zeiss®) with a spot size of 50um, power of 180mW, and exposure time of 100ms. Four laser burns were typically induced around the optic disc of each eye at the 3, 6, 9, and 12 o'clock positions. Tie2-specific antibody (clone #3) or isotype control antibody or anti-mouse VEGF control antibody (B20) was injected intraperitoneally (10mg / kg) 1 day before laser induction and every 3 days for a total of three injections. Nine days after laser induction, mice were perfused with FITC-lectin or TRITC-dextran via the tail vein. After 5 min of perfusion, the eyes were removed and fixed in 4% paraformaldehyde (PFA) for 15 min.

[0183] The choroid-sclera complex and retina were separated and anti-CD31 immunofluorescence (IF) was performed to reveal the vasculature by whole mount staining of both retina and choroid tissue. For CD31 IF, rat anti-mouse antibody BD550274 was diluted 1:100 and incubated overnight at 4°C. After 4 hours of incubation with secondary anti-rat antibody (Life Technologies, A11006), whole mounts were imaged at 488 nm. See Figure 9A. Quantification of neovascularization in the lesions and blood vessel density in the retina was performed by Image J. P values ​​were evaluated by Student's t-test (significant change, p<0.05). See Figure 9B.

[0184] As shown in Figures 9A and 9B, anti-Tie2 antibody, but not isotype control mAb, significantly inhibited the size of choroidal neovascular lesions after laser injury. This result indicates that activation of Tie2 after administration of anti-Tie2 mAb has the potential to provide significant clinical benefit to humans suffering from ophthalmic disorders such as AMD or diabetic retinopathy.

[0185] Example 12: Streptozotocin-induced (STZ) diabetic mouse model Administration of the agonist Tie2 antibodies of the invention may have utility for the treatment of diabetic retinopathy, as well as other pathological conditions associated with diabetes, such as nephropathy. To explore potential therapeutic benefits, the impact of the anti-Tie2 antibodies of the invention will be tested on disease-related endpoints, such as vascular leakage in the eye, visual function as assessed by electroretinogram, production of cytokines believed to be involved in human disease, such as IL-1b, and kidney function as measured by proteinuria in a streptozotocin-induced diabetic mouse model administered the mAb via the intraperitoneal route.

[0186] Six to seven week old C57BL / 6J mice can be weighed and their baseline blood glucose can be measured (Accu-Chek®, Roche). Mice can be injected intraperitoneally with STZ (Sigma-Aldrich, St. Louis, MO) at 55 mg / kg for five consecutive days. Age-matched controls can be injected with buffer alone. Blood glucose can be measured again one week after the last STZ injection, and mice are considered diabetic if non-fasting blood glucose is higher than 17 mM (300 mg / dL). STZ-treated diabetic C57BL / 6J mice can then be injected intravitreally (IVT) with an appropriate amount of an anti-Tie2 antibody of the invention, a control antibody, vehicle, or a comparison antibody, such as an anti-VEGF antibody or a recombinant VEGF fusion protein, at least eight weeks after STZ administration.

[0187] Electroretinograms (ERGs) evaluate the overall function of retinal cells using the UTAS-E visual electrodiagnostic testing system. After overnight dark adaptation, treated mice are anesthetized with a subcutaneous injection of BW of a cocktail containing ketamine and xylazine in sterile water. One eye is proptosed and the pupil is dilated with tropicamide and phenylephrine HCL. The eye is moistened with Genteal® eye drops and core body temperature is maintained using a heating pad. ERGs can be recorded using ultra-low impedance silver / nylon DTL plus electrodes. Needle electrodes can be placed at the center of the forehead and at the base of the tail. Gold contact lens electrodes are used to record ERG responses. Stimuli can consist of, for example, electronic 50 ms flashes in log steps. Responses are recorded from stimuli ranging from subthreshold to saturation. Analysis can include maximum amplitude and threshold of a-wave and b-wave.

[0188] Optokinetic tracking (OKT) can also be recorded as follows: Mice can be fixed on a turntable surrounded by a bank of synchronized monitors displaying a vertical black and white grating (14°). Sinusoidal vibration of the surrounding screen at 1 Hz and 10° / sec is applied to induce OKT. Right eye movements are monitored with an infrared-sensitive CCD camera under illumination by an ANA infrared LED47. Images are sampled at 200 Hz and the pupil center is calculated to estimate eye position using Morita's Geteye software program. OKT can be recorded three times for 30 s with approximately 30 s intervals.

[0189] Retinal vascular permeability can be measured as follows: Mice are anesthetized and Evans Blue dye dissolved in saline is injected via the tail vein. Two hours after tail vein injection, mice can be anesthetized with ketamine and xylazine and perfused through the left ventricle using saline. After perfusion, retinas are dissected, weighed, and placed in formamide for 18 hours at 70°C to extract the Evans Blue dye. The next day, retinas are centrifuged for 45 minutes and removed from the formamide. Evans Blue extravasation is measured using a plate reader at A620. A standard curve is used to convert to units of ng Evans Blue / wet tissue weight.

[0190] Tissue collection for targeted transcriptomics can be performed as follows: Mice are sacrificed under anesthesia and both eyes are removed. Retinas are then dissected, placed in RNAlater, processed, and analyzed by qRT-PCR.

[0191] Example 13: Bispecific biologic constructs Affecting both Tie2 and VEGFR-induced signaling pathways simultaneously may have enhanced benefits compared to regulating either pathway alone. To achieve this, bispecific constructs designed to affect both Tie2 and VEGFR were designed, expressed, and tested for activity. Figure 10A shows an exemplary schematic diagram of a bispecific antibody of the present invention with both Tie2-binding variable domain and VEGF-binding variable domain. Antibody clone #54 was designed and generated utilizing standard cloning techniques, and has the sequence of SEQ ID NO:282 and 283. A bispecific construct consisting of VEGFR R1D2 and R3D3 (VEGF trap protein) and Tie2-binding domain was also generated (antibody clone #55), and this construct has the sequence of SEQ ID NO:284. As described above in Example 3, Tie2 agonism was then assessed for two exemplary aTie2 / VEGF bispecific constructs (antibody clones #54 and #55) using 20 nM of each in HUVEC cells using the AlphaLISA™ screening platform designed to detect antibody-induced intracellular levels of pERK and pAkt.

[0192] The aTie2 / VEGF bispecific constructs tested were found to have the ability to induce Tie2 signaling in vitro (Figure 11). These bispecific molecules may allow for the administration of a single drug to patients with ocular disease and provide substantially greater efficacy than VEGF inhibition or Tie2 activation monotherapy. Promoting the regrowth of healthy vasculature provided by the Tie2 agonist component of the bispecific molecule may also be beneficial in mitigating potential adverse consequences of the anti-VEGF component of the molecule (i.e., geographic atrophy or missing capillaries in patients with ocular disease).

[0193] Example 14: Anti-Tie2 antibodies with increased valency and dual paratopic anti-Tie2 antibodies Antibodies with three or more Tie2 binding moieties, or antibodies capable of binding multiple epitopes on the Tie2 extracellular domain, may provide a means of enhancing activation of the Tie2 pathway in vivo to a greater extent than is possible with bivalent anti-Tie2 antibodies. Exemplary tetravalent anti-Tie2 constructs with the heavy and light chain sequences of anti-Tie2 antibody clone #3, with the addition of a polypeptide linker and B12 scFv sequence at the C-terminus of the heavy chain (antibody clone #51) or the N-terminus of the heavy chain (antibody clone #52), were generated using standard techniques. See Figures 12A-C for an exemplary schematic.

[0194] As described in Example 3, tetravalent antibody clones #51 and #52 were evaluated for their ability to induce signaling through the measurement of pAkt. As shown in FIG. 14, both clones #51 and #52 show improved ability to activate Tie2 signaling in HUVEC cells compared to bivalent anti-Tie2 constructs (aTie2) or compared to angiopoietin 1 (Ang1). Thus, anti-Tie2 variants with additional Tie2 binding moieties may provide a means to induce higher order oligomerization of Tie2 on the cell surface, potentially providing therapeutic benefit in situations where the level of agonism mediated by bivalent anti-Tie2 antibodies or the natural agonist ligand Ang1 is not adequate to restore vascular homeostasis in diseased tissues.

[0195] Example 15: Anti-Tie2 antibodies with Fc mutations that promote the ability to hexamerize on the cell surface Antibodies containing a specific mutation in the Fc domain of the heavy chain (i.e., E430G) have been demonstrated to enhance their ability to form hexamers on the cell surface (M. Overdijk, Mol Cancer Ther 2020;19:2126-38). An anti-Tie2 antibody containing an Fc domain with an E430G mutation was synthesized and compared to the same anti-Tie2 antibody with a comparable Fc containing a native glutamic acid (E) at position 430. See Figure 13 for a schematic diagram of such a hexamerized anti-Tie2 antibody.

[0196] Tie2 agonism was evaluated using pAKT / pERK Western blot as described in Example 3. As shown in Figure 14, the anti-Tie2 E430G construct (antibody clone #50) demonstrated improved ability to induce Tie2 signaling in HUVEC cells compared to the E430 construct. Thus, B12 variants with mutations that increase the likelihood of hexamerization at the cell surface may be a means to induce higher order oligomerization of Tie2 on the cell surface, potentially providing therapeutic benefit in situations where the level of agonism mediated by anti-Tie2 antibodies or the natural agonist ligand Ang1 is not adequate to restore vascular homeostasis in diseased tissues.

[0197] Example 16: Determination of Fab binding affinity of anti-Tie2 antibodies by SPR BIACORE™ (BIAcore, Inc., Piscataway, NJ) was performed at 25° C. with human, rat or mouse Tie2 ECD antigen immobilized on a carboxymethylated dextran biosensor chip (CM5) at approximately 10 response units (RU). CM5 chips were activated with N-ethyl-N′-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) according to the supplier's instructions. All Tie2 ECD antigens were diluted to 5 μg / ml (approximately 0.2 μM) in 10 mM sodium acetate, pH 4.8, and then injected at a flow rate of 5 μl / min to obtain approximately 10 RU of coupled protein. After each Tie2 ECD injection, 1 M ethanolamine was injected to block unreacted groups. For kinetic measurements, two-fold serial dilutions (0.78 nM to 500 nM) of the Fab of antibody clone #3 were injected in PBS containing 0.05% polysorbate 20 (TWEEN™-20) surfactant (PBST) at 25° C. at a flow rate of approximately 25 μl / min. Association rates (kon) and dissociation rates (koff) were calculated using a simple one-to-one Langmuir binding model (BIACORE™ Evaluation Software version 3.2) by simultaneously fitting the association and dissociation sensorgrams. Equilibrium dissociation constants (KD) were calculated as the ratio of koff / kon. See, e.g., Chen et al., J. Mol. Biol. 293:865-881 (1999).

[0198] The table below shows the Fab binding affinity of antibody clone #3 to each Tie2 ECD antigen tested. TIFF0007638989000011.tif26166

[0199] The foregoing invention has been described in some detail by way of illustration and example, for purposes of clarity of understanding, but the descriptions and examples should not be construed as limiting the scope of the invention. The disclosures of all patent and scientific literature cited herein are expressly incorporated by reference in their entireties.

Claims

1. An isolated anti-Tie2 antibody or antigen-binding fragment thereof comprising three heavy chain complementarity determining regions (CDRs H1-3) and three light chain CDRs (CDRs L1-3), wherein CDR H1 comprises the amino acid sequence of SEQ ID NO:1, CDR H2 comprises the amino acid sequence of SEQ ID NO:41, CDR H3 comprises the amino acid sequence of SEQ ID NO:81, CDR L1 comprises the amino acid sequence of SEQ ID NO:121, CDR L2 comprises the amino acid sequence of SEQ ID NO:161, and CDR L3 comprises the amino acid sequence of SEQ ID NO:

201.

2. An isolated anti-Tie2 antibody or antigen-binding fragment thereof, comprising a heavy chain variable (VH) domain comprising the amino acid sequence of SEQ ID NO:246, and a light chain variable (VL) domain comprising the amino acid sequence of SEQ ID NO:

247.

3. The antibody of claim 1 which is an allosteric activator of Tie2.

4. The antibody of claim 1 which is a non-ligand competitive binder of Tie2.

5. The antibody of claim 1 , which is cross-reactive with human, mouse, rat, rabbit and monkey Tie2.

6. The antibody of claim 1, which is a fully human antibody, a humanized antibody, a monoclonal antibody, or a chimeric antibody.

7. The antibody of claim 1, which is an antibody fragment that specifically binds to human Tie2.

8. The antibody fragment is Fab, Fab'-SH, Fv, scFv, or (Fab') ２ The antibody of claim 7 which is a fragment.

9. The antibody of claim 1, which possesses reduced effector function.

10. 10. The antibody of claim 9, comprising at least one substitution mutation at amino acid residues N297, L234, L235, P329, D265, and E430 according to EU numbering, such as Kabat numbering.

11. 11. The antibody of claim 10, wherein the at least one substitution mutation is selected from the group consisting of amino acid residues N297G, N297A, L234A, L235A, P329G, D265A, and E430G according to EU numbering, such as Kabat numbering.

12. 12. The antibody of claim 11, comprising the substitution mutation at residue N297A or N297G.

13. The antibody of claim 11, comprising the substitution mutations at residues L234A, L235A, and P329G.

14. The antibody of claim 11, comprising the substitution mutations at residues D265A and N297G.

15. An isolated nucleic acid encoding the antibody of claim 1.

16. A vector comprising the isolated nucleic acid of claim 15.

17. A host cell comprising the vector of claim 16.

18. A method for producing the antibody of claim 1, comprising: Culturing the host cell of claim 17 in a culture medium; Isolating the resulting antibody. A method comprising:

19. An immunoconjugate or fusion polypeptide comprising the antibody of claim 1.

20. 20. A pharmaceutical composition comprising the antibody of claim 1 or the immunoconjugate or fusion polypeptide of claim 19.

21. 21. The pharmaceutical composition of claim 20 for treating a Tie2 dysregulation disease.

22. 22. The pharmaceutical composition of claim 21, wherein the Tie2 dysregulated disease comprises infectious disease, acute respiratory distress syndrome (ARDS), ischemic injury, eye damage, radiation injury, cancer, systemic sclerosis, traumatic brain injury, neuroinflammation, radiation injury, wound healing, myocardial infarction, blood-brain barrier damage, cerebral cavernous malformation, Duchenne muscular dystrophy (DMD) or Clarkson's disease.

23. 23. The pharmaceutical composition of claim 22, wherein the Tie2 dysregulated infection comprises sepsis, dengue virus infection, tuberculosis, or influenza.

24. 23. The pharmaceutical composition of claim 22, wherein the Tie2 dysregulated ischemic injury comprises diabetic nephropathy, acute kidney injury, chronic kidney disease, organ transplantation, critical limb ischemia, traumatic brain injury or stroke.

25. 23. The pharmaceutical composition of claim 22, wherein the Tie2 dysregulated ocular disorder comprises diabetic retinopathy, diabetic macular edema (DME), proliferative diabetic retinopathy (PDR), age-related macular degeneration (AMD), retinopathy of prematurity (ROP), or glaucoma.

26. Use of an isolated anti-Tie2 antibody according to any one of claims 1 to 14, or an immunoconjugate or fusion polypeptide according to claim 19, for the manufacture of a medicament for treating a Tie2 dysregulation disease according to any one of claims 22 to 25.

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