Calyspongiolide, its analogs, and their uses

Abstract

Methods for producing calispongiolide derivatives are provided, particularly for treating various types of cancer. The compound of formula (I) is synthesized by Sonogashira coupling of the corresponding acetylene compound with a vinyl iodide. TIFF2022120170000087.tif37128

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Patent Application No. 62 / 342,601, filed May 27, 2016, the entirety of which is incorporated by reference as if fully set forth herein. [Background technology]

[0002] 2. Background of the Invention Marine sponges of the genus Callyspongia have been found to be a rich source of natural products that exhibit cytotoxic activity. Examples of such natural products include callyazepin, a nitrogen-containing macrocycle that exhibits moderate cytotoxicity against K562 and A549 cell lines, and a methanol extract of the marine sponge species Callyspongia spp., which showed complete inhibition of the murine lymphoma cell line L5178Y. In addition, testing of callyspongiolide against human Jurkat J16 T lymphocytes and Ramos B lymphocytes showed IC values ​​of 70 and 60 nM, respectively. 50 The values ​​were shown. Summary of the Invention

[0003] [The present invention 1001] Formula (IV): The compound of formula (V): contacting the compound of formula (I) with the compound of formula (I). Formula (III): 1. A method for making a compound of TIFF0007641933000003.tif37128 or a pharma- ceutically acceptable salt, polymorph, prodrug, solvate, or clathrate thereof, comprising: During the ceremony, each dashed bond independently represents a single bond or a double bond, and when a dashed bond represents a double bond, the double bond can have an E or Z configuration; R 1 is H, alkyl, R 2 -(CH2) q -X 3 -, R 2 -(CH2) q -C(O)NR 6 (CH2) q -X 3 -, OR 6 , or N(R 6 )2, where R 6 each independently represents H, alkyl, aryl, alkaryl, or arylalkyl, and each q is independently an integer from 0 to 9; X 1 , O, NR 6 , or S; X 2 , O, NR 6 , or S; R 2 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 )2, where p is an integer from 0 to 2 and m is an integer from 1 to 10; X 3 , O, NR 6 , S(O) p , or C(R 6 )2; X 4 O, NR 6 , or S, or X 4 and X 4 If there is a single bond between the carbon atom to which X is bonded, 4 is R 2 and; R 3 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 )2; R 4is H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , N(R 6 )2, or X 1 C(O)(CH) q R 6 and R 5 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 ) 2, and n is an integer from 0 to 4. [The present invention 1002] The method of claim 1001, wherein m is 3 and / or n is 2. [The present invention 1003] The compound of formula (III) has the formula: As in the compound of formula (IV) of (X): The method of the present invention 1001, which is a compound of TIFF0007641933000005.tif36128. [The present invention 1004] The compound of formula (III) has the formula: As the compound of formula (V) is a compound of formula (XI): The method of the present invention 1001, which is a compound of TIFF0007641933000007.tif30128. [The present invention 1005] The compound of formula (III) has the formula: The method of the present invention 1001, which is a compound of TIFF0007641933000008.tif33128. [The present invention 1006] The compound of formula (III) has the formula: The method of the present invention 1001, which is a compound of TIFF0007641933000009.tif33128. [The present invention 1007] The compound of formula (III) has the formula: The method of the present invention 1001, which is a compound of TIFF0007641933000010.tif33128. [The present invention 1008] X 1 , X 2 , X 3 , and X 4 The method according to any one of claims 1001 to 1007, wherein each of [The present invention 1009] The method of claim 1008, wherein each dashed bond represents a double bond in the configuration shown. [The present invention 1010] R 1 N(R 6 )2, and R 6 1001. The method of claim 1001, wherein each independently represents H, alkyl, aryl, alkaryl, or arylalkyl. [The present invention 1011] R 2 The process of claim 1001, wherein each is independently C1 to C6 alkyl. [The present invention 1012] R 5 each independently represents halo or OR 6 The method of the present invention 1001. [The present invention 1013] R 3 The process of claim 1001, wherein each is independently C1 to C6 alkyl. [The present invention 1014] R 4 OR 6 The method of the present invention 1001. [The present invention 1015] The compound of formula (III) has the formula (I): The method of the present invention 1001, which is a compound of TIFF0007641933000011.tif37128. [The present invention 1016] Formula (III): Compound of TIFF0007641933000012.tif37128 or a pharma- ceutically acceptable salt, polymorph, prodrug, solvate, or clathrate thereof: During the ceremony, each dashed bond independently represents a single bond or a double bond, and when a dashed bond represents a double bond, the double bond can have an E or Z configuration; R 1 is H, alkyl, R 2 -(CH2) q -X 3 -, R 2 -(CH2) q -C(O)NR 6 (CH2) q -X 3 -, OR 6 , or N(R 6 )2, where R 6 each independently represents H, alkyl, aryl, alkaryl, or arylalkyl, and each q is independently an integer from 0 to 9; X 1 , O, NR 6 , or S; X 2 , O, NR 6 , or S; R 2 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 )2, where p is an integer from 0 to 2 and m is an integer from 1 to 10; X 3 , O, NR 6 , S(O) p , or C(R 6 )2; X 4 O, NR 6 , or S, or X 4 and X 4 If there is a single bond between the carbon atom to which X is bonded, 4 is R 2 and; R 3 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR6 , S(O) p R 6 , or N(R 6 )2; R 4 is H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , N(R 6 )2, or X 1 C(O)(CH) q R 6 and R 5 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 ) 2, n is an integer of 0 to 4, and the compound of formula (III) is a compound of formulas (I) and (II): Not the compound TIFF0007641933000013.tif75128. [The present invention 1017] A pharmaceutical composition comprising a compound of the present invention and a pharma- ceutically acceptable carrier. [The present invention 1018] A method for treating cancer, comprising administering to a subject in need thereof a therapeutically effective amount of one or more compounds of the present invention 1016 or a pharmaceutical composition comprising one or more compounds of the present invention 1016. [The present invention 1019] A compound of the present invention for use as a medicament for the treatment of a patient in need of palliation of cancer. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0004] Detailed Description of the Invention In the continuing interest of synthesizing bioactive natural products and considering novel structures of calyspongiolide macrolides, we aimed to establish a convergent and enantioselective synthesis of calyspongiolide to aid in the structure determination and to further explore the biological studies of not only calyspongiolide but also analogues of this compound.

[0005] Reference will now be made in detail to certain examples of the disclosed subject matter, examples of which are illustrated in part in the accompanying drawings. While the disclosed subject matter will be described in conjunction with the enumerated claims, it will be understood that the exemplified subject matter is not intended to limit the scope of the claims to the disclosed subject matter.

[0006] Various examples described herein are represented by formulas (I) and (II): The present invention is directed to a compound of Formula (I) and / or (II) of the formula (I) or a pharma- ceutically acceptable salt, polymorph, prodrug, solvate, or clathrate thereof, and methods of making and using the compounds of Formula (I) and / or (II) for treating various types of cancer (e.g., chronic myeloid leukemia, lung cancer, lymphoma, T-acute lymphoblastic leukemia, and Burkitt's lymphoma), among others.

[0007] Various other examples described herein include compounds of formula (III): The present invention is directed to a compound of formula (III) or a pharma- ceutically acceptable salt, polymorph, prodrug, solvate, or clathrate thereof, and methods of making and using the compound of formula (III) or a pharma- ceutically acceptable salt, polymorph, prodrug, solvate, or clathrate thereof, inter alia, for treating various types of cancer, During the ceremony, each dashed bond independently represents a single bond or a double bond, and when a dashed bond represents a double bond, the double bond can have an E or Z configuration; R 1 is H, alkyl, R 2 -(CH2) q -X3 -, R 2 -(CH2) q -C(O)NR 6 (CH2) q -X 3 -, OR 6 , or N(R 6 )2, where R 6 each independently represents H, alkyl, aryl, alkaryl, or arylalkyl, and each q is independently an integer from 0 to 9; X 1 , O, NR 6 , or S; X 2 , O, NR 6 , or S; R 2 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 )2, where p is an integer from 0 to 2 and m is an integer from 1 to 10 (e.g., m is 1 to 5, 2 to 5, 3 to 5, or 3 to 7, and / or n is 2: in some examples, m is 3 and / or n is 2); X 3 , O, NR 6 , S(O) p , or C(R 6 )2; X 4 O, NR 6 , or S, or X 4 and X 4 If there is a single bond between the carbon atom to which X is bonded, 4 is R 2 and; R 3 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 )2; R 4is H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , N(R 6 )2, or X 1 C(O)(CH) q R 6 and; R 5 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 ) 2, where n is an integer from 0 to 4.

[0008] In some instances, the compound of formula (III) is not a compound of formula (I) and / or (II).Also contemplated herein is an antibody conjugated to a compound of formula (III) via any suitable point in the molecule (e.g., via an ether, ester, or amide bond).

[0009] In some examples, the compound of formula (III) has the formula: The compound is TIFF0007641933000016.tif180128.

[0010] In some instances, X 1 , X 2 , X 3 , and X 4 is O. In other examples, each dashed bond represents a double bond in the configuration shown in the compound of formula (III). 1 is N(R 6 )2, where R 6 each independently represents H, alkyl, aryl, alkaryl, or arylalkyl. 1 is the group R 2 -(CH2) q -X 3 - or R 2 -(CH2) q-C(O)NR 6 (CH2) q -X 3 -, where each q is independently an integer from 0 to 9 (e.g., 1 to 9, 1 to 8, 0 to 5, and 0 to 8). 2 -(CH2) q -X 3 - For example, the wavy line is X 1 A carbon atom bearing a group R 1 The group that is the point of attachment of: TIFF0007641933000017.tif26128 etc. Group Heterocyclyl-(CH2) q -X 3 - (wherein each q is independently an integer of 0 to 9). 2 -(CH2) q -C(O)NR 6 (CH2) q -X 3 As an example, the group: TIFF0007641933000018.tif31128 etc. Group Heterocyclyl-(CH2) q -C(O)NR 6 (CH2) q -X 3 - (wherein each q is independently an integer of 0 to 9).

[0011] In some instances, R 2 are each independently C1-C6 alkyl (e.g., methyl, ethyl, or propyl). 5 are each independently halo or OR 6 In yet another example, R 3 are each independently C1 to C6 alkyl. In other examples, R 4 OR 6 In yet another example, R 4 isX 1 C(O)(CH) q R 6 The group X 1 C(O)(CH) q R 6 As an example, R 6 but TIFF0007641933000019.tif15128-NH-C(O)-(CH2) q -R 6 (wherein each q is independently an integer of 0 to 9).

[0012] In some examples, the compound of formula (III) is Formula (IV): The compound of formula (V): contacting (e.g., reacting) the compound of TIFF0007641933000021.tif29128 with In the formula, the substituent R 1 ~R 6 and X 1 ~X 4 is defined herein.

[0013] In some examples, the compounds of formula (IV) and (V) have formula (VI) and (VII), respectively: It can be synthesized from the compound TIFF0007641933000022.tif77128, In the formula, the substituent R 1 ~R 6 and X 1 ~X 4 is defined herein, and P 1 and P 2 are independently suitable oxygen protecting groups. For other commonly used protecting groups for hydroxyl groups, see Peter GM Wuts and Theodora W. Greene, Greene's Protective Groups in Organic Synthesis (4 th ed. 2007). For example, see OP 2 The group is NR 6 P 3 P is substituted with 3 By using a compound in which X is a suitable amino protecting group, 2It should be understood that the compound of formula (VI) can be modified to access compounds where is other than O.

[0014] In some examples, the compounds of formula (VI) and (VII) have formula (VIII) and (IX), respectively: It can be synthesized from the compound TIFF0007641933000023.tif55128, In the formula, the substituent R 1 ~R 6 and X 1 ~X 4 is defined herein, and P 1 and P 4 are independently suitable oxygen protecting groups. For other commonly used protecting groups for hydroxyl groups, see Peter GM Wuts and Theodora W. Greene, Greene's Protective Groups in Organic Synthesis (4 th ed. 2007). For example, see OP 4 The group is NR 6 P 5 P is substituted with 5 By using a compound in which X is a suitable amine protecting group, 3 It should be understood that the compound of formula (VIII) can be modified to obtain compounds in which is other than O. In addition, one or more groups R 2 X 2 It should be understood that the ring may also be on a ring containing:

[0015] In some examples, the compound of formula (III) has the formula: As shown in TIFF0007641933000024.tif34128, the compound has the formula (X): An example of the method is to prepare a compound of formula (III) using a compound of TIFF0007641933000025.tif36128.

[0016] In another embodiment, the compound of formula (III) has the formula: As the compound of formula (XI): An example of the method is to prepare a compound of formula (III) using a compound of TIFF0007641933000027.tif30128.

[0017] As yet another example, the formula: A method of preparing a compound of formula (III) using a compound of formula (X) and a compound of formula (XI) to give a compound of formula (TIFF0007641933000028.tif34128 is also included.

[0018] Those of ordinary skill in the art will recognize that the compounds described herein may contain chiral centers. All diastereomers and racemates of the compounds described herein are contemplated.

[0019] The various examples of the present invention also contemplate pharmaceutical compositions comprising one or more compounds of the various examples of the present invention (e.g., compounds of formulas (I)-(III)) and one or more pharmaceutical carriers, diluents, excipients, or mixtures thereof. A "pharmaceutical composition" refers to a chemical or biological composition suitable for administration to a subject (e.g., a mammal). Such compositions may be specifically formulated for administration via one or more routes, including, but not limited to, buccal, dermal, epidermal, epidural, injection, inhalation, intraarterial, intracardiac, intraventricular, intradermal, intramuscular, intranasal, intraocular, intraperitoneal, intraspinal, intrathecal, intravenous, oral, parenteral, pulmonary, rectal by enema or suppository, subcutaneous, subdermal, sublingual, transdermal, and mucosal. Additionally, administration may be by capsule, drop, foam, gel, gum, injection, liquid, patch, pill, porous pouch, powder, tablet, or other suitable means of administration.

[0020] A "pharmaceutical excipient" or "pharmaceutical acceptable excipient" includes a carrier, possibly a liquid, in which an active therapeutic agent is formulated. An excipient may provide chemical and / or biological stability and release characteristics, but typically does not provide any pharmacological activity to the formulation. Examples of suitable formulations can be found, for example, in Remington, The Science And Practice of Pharmacy, 20th Edition, (Gennaro, AR, Chief Editor), Philadelphia College of Pharmacy and Science, 2000, the entire contents of which are incorporated herein by reference.

[0021] As used herein, "pharmaceutically acceptable carrier" or "excipient" includes any and all physiologically compatible solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents. In one example, the carrier is suitable for parenteral administration. Alternatively, the carrier is suitable for intravenous, intraperitoneal, intramuscular, sublingual, or oral administration. Pharmaceutically acceptable carriers include sterile aqueous solutions or dispersions, and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, its use in the pharmaceutical compositions of the present invention is contemplated. Additional active compounds can also be incorporated into the compositions.

[0022] Pharmaceutical compositions can be sterile and stable under the conditions of manufacture and storage.The compositions can be formulated as solution, microemulsion, liposome, or other ordered structure suitable for high drug concentration.Carrier can be solvent or dispersion medium, for example, containing water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol), and suitable mixtures thereof.Proper fluidity of solution can be maintained, for example, by using coating such as lecithin, by maintaining required particle size in case of dispersion, and by using surfactant.

[0023] In many cases, it will be preferable to include an isotonic agent in the composition, for example, sugars, polyalcohols such as mannitol, sorbitol, or sodium chloride. Prolonged absorption of an injectable composition can be achieved by including in the composition an agent that delays absorption, for example, monostearate salts and gelatin. Furthermore, the compounds described herein can be formulated into sustained release formulations, for example, compositions containing slow-release polymers. The active compounds can be prepared with carriers that will protect the compound from rapid release, such as controlled release formulations, including implants and microencapsulated delivery devices. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, polylactic acid, and polylactic-polyglycolic acid copolymers (PLG). Many methods for preparing such formulations are known to those skilled in the art.

[0024] Oral forms of administration are also contemplated herein. The pharmaceutical compositions of the present invention may be orally administered as capsules (hard or soft capsules), tablets (film-coated, enteric-coated or plain), powders or granules (coated or uncoated), or liquids (solutions or suspensions). The formulations may be conveniently prepared by any of the methods well known in the art. The pharmaceutical compositions of the present invention may include one or more suitable processing aids or excipients, including fillers, binders, disintegrants, lubricants, diluents, flow agents, buffers, wetting agents, preservatives, colorants, sweeteners, flavoring agents, and pharma- ceutical compatible carriers.

[0025] For each of the examples described, the compound can be administered in various dosage forms known in the art.Any biologically acceptable dosage form known to those skilled in the art and combinations thereof are contemplated.Examples of such dosage forms include, but are not limited to, chewable tablets, fast-dissolving tablets, effervescent tablets, reconstitutable powders, elixirs, liquids, solutions, suspensions, emulsions, tablets, multi-layer tablets, bi-layer tablets, capsules, soft gelatin capsules, hard gelatin capsules, caplets, lozenges, chewable lozenges, beads, powders, gums, granules, particles, microparticles, dispersible granules, cachets, irrigation solutions, suppositories, creams, topicals, inhalants, aerosol inhalants, patches, particle inhalants, implants, depot implants, ingestibles, injections (including subcutaneous, intramuscular, intravenous, and intradermal injections), infusions, and combinations thereof.

[0026] Other compounds which may be included by mixing are, for example, medicamentarily inactive ingredients (e.g., solid and liquid diluents) such as lactose, dextrose, sucrose, cellulose, starch or calcium phosphate for tablets or capsules, olive oil or ethyl oleate for soft capsules, and water or vegetable oil for suspensions or emulsions; lubricants such as silica, talc, stearic acid, magnesium or calcium stearate, and / or polyethylene glycol; gelling agents such as colloidal clays; thickening agents such as gum tragacanth or sodium alginate, binding agents such as starch, gum arabic, gelatin, methylcellulose, carboxymethylcellulose or polyvinylpyrrolidone; disintegrating agents such as starch, alginic acid, alginates or sodium starch glycolate; saturants; dyes; sweeteners; wetting agents such as lecithin, polysorbates or lauryl sulfate; and other therapeutically acceptable minor ingredients which are known additives for such formulations, such as humectants, preservatives, buffers and antioxidants.

[0027] The liquid dispersion for oral administration can be a syrup, an emulsion, a solution, or a suspension.Syrups can contain, as a carrier, for example, sucrose, or sucrose with glycerol and / or mannitol and / or sorbitol.Suspensions and emulsions can contain carriers, for example, natural gum, agar, sodium alginate, pectin, methylcellulose, carboxymethylcellulose, or polyvinyl alcohol.

[0028] The amount of active compound in the therapeutic composition according to various embodiments of the present invention may vary according to factors such as disease state, age, sex, body weight, patient history, risk factors, disease predisposition, administration route, previous treatment plan (e.g., possible interactions with other medications) and individual weight. The dosage plan may be adjusted to provide optimal therapeutic response. For example, a single bolus may be administered, or several divided doses may be administered over a period of time, or the dosage may be proportionally reduced or increased according to the requirements of the therapeutic situation.

[0029] As used herein, "dosage unit form" refers to physically discrete units suited as unitary dosages for the mammalian subject to be treated, each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect, together with the required pharmaceutical carrier. The specifications for the dosage unit forms of the present invention are dictated by and directly depend on the unique characteristics of the active compound and the particular therapeutic effect to be achieved, as well as the limitations inherent in the art of compounding such active compounds for the treatment of sensitivities in individuals. In therapeutic use for the treatment of conditions in mammals (e.g., humans) for which a compound of the present invention or a suitable pharmaceutical composition thereof is effective, the compound of the present invention may be administered in an effective amount. The dosage suitable for the present invention may be a composition, pharmaceutical composition or any other composition described herein.

[0030] For each of the described examples, dosages are typically administered once, twice, or three times a day, although more frequent dosing intervals are possible. Dosages may be administered daily, every second day, every third day, every fourth day, every fifth day, every sixth day, and / or every seventh day (once a week). In one example, dosages may be administered daily for up to and including 30 days, preferably for 7-10 days. In another example, dosages may be administered twice a day for 10 days. If a patient is in need of treatment for a chronic disease or condition, dosages may be administered as long as signs and / or symptoms persist. Patients may require "maintenance therapy," in which case the patient will receive daily dosages for months, years, or the remainder of the patient's life. In addition, the compositions of the present invention may be for the effective prevention of recurrent symptoms. For example, dosages may be administered once or twice a day to prevent the onset of symptoms in at-risk patients, particularly asymptomatic patients.

[0031] The compositions described herein can be administered by any of the following routes: oral, transdermal, epidural, injection, inhalation, intra-arterial, intracardiac, intraventricular, intradermal, intramuscular, intranasal, intraocular, intraperitoneal, intraspinal, intrathecal, intravenous, oral, parenteral, pulmonary, rectal by enema or suppository, subcutaneous, subdermal, sublingual, transdermal, and transmucosal. Preferred routes of administration are oral and oral. Administration can be local, where the composition is administered directly adjacent to, localized to, in the immediate vicinity of, at, near, or near the site of disease, e.g., inflammation, or administration can be systemic, where the composition is given to the patient and passes generally through the body, thereby reaching the site of disease. Local administration can be administered to cells, tissues, organs, and / or organ systems that contain and / or are affected by disease and / or where signs and / or symptoms of disease are active or may occur. Administration can be topical, with a local effect, where the composition is applied directly to the location where its action is desired; administration can be enteral, where the desired effect is systemic (non-local), where the composition is administered through the digestive tract; administration can be parenteral, where the desired effect is systemic, where the composition is administered by a route other than the digestive tract.

[0032] In some instances, the various examples of the present invention contemplate compositions comprising a therapeutically effective amount of one or more compounds of the various examples of the present invention. In some aspects, the various examples of the present invention contemplate compounds of Formulas (I)-(III) for use as medicaments for the treatment of patients in need of relief from a disease or condition, such as cancer.

[0033] The term "therapeutically effective amount" as used herein refers to an amount of one or more of the various examples of the compound of the present invention that elicits the biological or medical response in a tissue system, animal or human that is desired by a researcher, veterinarian, physician or other clinician, including the alleviation of symptoms of the disease or disorder being treated. In some examples, a therapeutically effective amount is an amount that can treat or alleviate a disease or symptoms of a disease with a reasonable benefit / risk ratio applicable to any medical treatment. However, it should be understood that the total daily usage of the compounds and compositions described herein can be determined by the attending physician within the scope of sound medical judgment. For any particular patient, the specific therapeutically effective dose level will depend on a variety of factors, including factors well known to a researcher, veterinarian, physician or other clinician, such as the condition being treated and the severity of that condition; the activity of the specific compound used; the specific composition used; the age, weight, general health, sex and diet of the patient; the time of administration, the route of administration, the rate of excretion of the specific compound used; the duration of treatment; drugs used in combination with or simultaneously with the specific compound used, etc. It will also be appreciated that a therapeutically effective amount can be selected based on any toxicity or other undesirable side effects that may arise during administration of one or more of the compounds described herein.

[0034] In some examples, the compounds of various examples of the present invention have a half-maximal inhibitory concentration (IC) of about 5 nM to about 500 nM (e.g., about 50 nM to about 100 nM, about 10 nM to about 75 nM, about 10 nM to about 60 nM, about 100 nM to about 500 nM, about 50 μM to about 250 nM, about 100 nM to about 300 nM, or about 10 nM to about 30 nM). 50 ).

[0035] Values ​​expressed in range format should be interpreted flexibly to include not only the numerical values ​​explicitly stated as the boundaries of the range, but also all individual numerical values ​​or subranges subsumed within the range as if each numerical value and subrange were explicitly stated. For example, the range "about 0.1% to about 5%" or "about 0.1% to 5%" should be interpreted to include not only about 0.1% to about 5%, but also individual values ​​(e.g., 1%, 2%, 3%, and 4%) and subranges (e.g., 0.1% to 0.5%, 1.1% to 2.2%, 3.3% to 4.4%) within the stated range. The term "about X to Y" has the same meaning as "about X to about Y" unless otherwise indicated. Similarly, the term "about X, Y, or about Z" has the same meaning as "about X, about Y, or about Z" unless otherwise indicated.

[0036] As used herein, the terms "a," "an," or "the" are used to include one or more, unless the context clearly dictates otherwise. The term "or" is used to refer to a non-exclusive "or," unless otherwise indicated. In addition, any phrase or term used herein, unless otherwise defined, should be understood to be for descriptive purposes only and not for limiting purposes. Any use of section headings is intended to aid in the interpretation of the specification and should not be construed as limiting. Furthermore, information associated with a section heading may be found within or outside that particular section. Furthermore, all publications, patents, and patent documents referred to herein are incorporated herein by reference in their entirety, as if each was incorporated by reference individually.

[0037] In the methods described herein, steps may be performed in any order without departing from the principles of the invention, unless a chronological or operational order is explicitly recited. Moreover, specified steps may also be performed simultaneously, unless express claim language recites that they are performed separately. For example, a step defined as doing X and a step defined as doing Y may be performed simultaneously in a single operation, and the resulting process would be included within the literal scope of the defined process.

[0038] As used herein, the term "about" can allow for a variation in value or range, for example, within 10%, within 5%, or within 1% of a stated value or of the limits of a stated range.

[0039] The term "substituted" as used herein refers to a group (e.g., alkyl, aryl, and heteroaryl) or molecule on which one or more hydrogen atoms contained therein are replaced by one or more substituents. The term "substituent" as used herein refers to a group that can be or is substituted on a molecule or group. Examples of substituents include, but are not limited to, halogens (e.g., F, Cl, Br, and I); oxygen atoms in groups such as hydroxy groups, alkoxy groups, aryloxy groups, aralkyloxy groups, oxo(carbonyl) groups, carboxy groups including carboxylic acids, carboxylates, and esters; sulfur atoms in groups such as thiol groups, alkyl and aryl sulfide groups, sulfoxide groups, sulfone groups, sulfonyl groups, and sulfonamide groups; nitrogen atoms in groups such as amines, hydroxylamines, nitriles, nitro groups, N-oxides, hydrazides, azides, and enamines; and other heteroatoms in various other groups. Non-limiting examples of substituents that may be attached to a substituted carbon (or other) atom include F, Cl, Br, I, OR, OC(O)N(R), CN, NO, NO, ONO, azido, CF, OCF, R, O(oxo), S(thiono), C(O), S(O), methylenedioxy, ethylenedioxy, N(R), SR, SOR, SO, SO, N(R), SO, R, C(O)R, C(O)C(O)R, C(O)CHC(O)R, C(S)R, C(O)OR, OC(O)R, C(O)N(R), OC(O)N(R), C(S)N(R), (CH) 0~2 N(R)C(O)R, (CH2) 0~2N(R)N(R), N(R)N(R)C(O)R, N(R)N(R)C(O)OR, N(R)N(R)CON(R), N(R)SOR, N(R)SON(R), N(R)C(O)OR, N(R)C(O)R, N(R)C(S)R, N(R)C(O)N(R), N(R)C(S)N(R), N(COR)COR, N(OR)R, C(=NH)N(R), C(O)N(OR)R, or C(=NOR)R, where R can be, for example, hydrogen, alkyl, acyl, cycloalkyl, aryl, aralkyl, heterocyclyl, heteroaryl, or heteroarylalkyl.

[0040] As used herein, the term "alkyl" refers to an alkyl group having 1 to 40 carbon atoms (C 40 ), 1 to about 20 carbon atoms (C1 to C 20 ), 1 to 12 carbon atoms (C1 to C 12 ), 1 to 8 carbon atoms (C1-C8), or in some examples, 1 to 6 carbon atoms (C1-C6), substituted or unsubstituted straight chain and branched alkyl and cycloalkyl groups. Examples of straight chain alkyl groups include those having 1 to 8 carbon atoms, such as methyl, ethyl, n-propyl, n-butyl, n-pentyl, n-hexyl, n-heptyl, and n-octyl groups. Examples of branched alkyl groups include, but are not limited to, isopropyl, isobutyl, sec-butyl, t-butyl, neopentyl, isopentyl, and 2,2-dimethylpropyl groups. Representative substituted alkyl groups may be substituted one or more times with any of the groups enumerated herein, such as amino, hydroxy, cyano, carboxy, nitro, thio, alkoxy, and halogen groups.

[0041] The term "cycloalkyl" as used herein refers to substituted or unsubstituted cyclic alkyl groups, such as, but not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl groups. In some examples, cycloalkyl groups can have from 3 to about 8-12 ring atoms, while in other examples, the number of ring carbon atoms ranges from 3 to 4, 5, 6, or 7. In some examples, cycloalkyl groups can have 3 to 6 carbon atoms (C3-C6). Cycloalkyl groups further include polycyclic cycloalkyl groups, such as, but not limited to, norbornyl, adamantyl, bornyl, camphenyl, isocamphenyl, and carenyl groups, and fused rings, such as, but not limited to, decalinyl.

[0042] The term "acyl" as used herein refers to a group containing a carbonyl moiety bonded through a carbonyl carbon atom. The carbonyl carbon atom is also bonded to another carbon atom, which may be part of a substituted or unsubstituted alkyl, aryl, aralkyl, cycloalkyl, cycloalkylalkyl, heterocyclyl, heterocyclylalkyl, heteroaryl, heteroarylalkyl group, etc. In a particular example where the carbonyl carbon atom is bonded to a hydrogen, the group is a "formyl" group, which is an acyl group as the term is defined herein. An acyl group may contain 0 to about 12-40, 6-10, 1-5, or 2-5 additional carbon atoms bonded to the carbonyl group. An acryloyl group is an example of an acyl group. An acyl group may also contain heteroatoms within the meaning herein. A nicotinoyl group (pyridyl-3-carbonyl) is an example of an acyl group within the meaning herein. Other examples include acetyl, benzoyl, phenylacetyl, pyridylacetyl, cinnamoyl, and acryloyl groups. When the group containing the carbon atom that is bonded to the carbonyl carbon atom contains a halogen, the group is referred to as a "haloacyl" group. An example is the trifluoroacetyl group.

[0043] The term "aryl" as used herein refers to a substituted or unsubstituted cyclic aromatic hydrocarbon that does not contain a heteroatom in the ring. Thus, aryl groups include, but are not limited to, phenyl, azulenyl, heptalenyl, biphenyl, indacenyl, fluorenyl, phenanthrenyl, triphenylenyl, pyrenyl, naphthacenyl, chrysenyl, biphenylenyl, anthracenyl, and naphthyl groups. In some examples, aryl groups have from about 6 to about 14 carbons (C6 to C8) in the ring portion of the group. 14 ) or 6 to 10 carbon atoms (C6 to C 10 ). Aryl groups can be unsubstituted or substituted as defined herein. Representative substituted aryl groups can be mono-substituted or substituted more than once, such as, for example, 2-, 3-, 4-, 5-, or 6-substituted phenyl or 2-8-substituted naphthyl groups, which can be substituted with carbon or non-carbon groups, such as, but not limited to, those enumerated herein.

[0044] As used herein, the terms "aralkyl" and "arylalkyl" refer to an alkyl group, as defined herein, in which a hydrogen or carbon bond of the alkyl group is replaced with a bond to an aryl group, as defined herein. Representative aralkyl groups include benzyl and phenylethyl groups.

[0045] The term "heterocyclyl" as used herein refers to substituted or unsubstituted aromatic and non-aromatic ring compounds containing three or more ring members, one or more of which are heteroatoms, such as, but not limited to, N, O, and S. Thus, a heterocyclyl can be a cycloheteroalkyl or heteroaryl, or any combination thereof if polycyclic. In some examples, heterocyclyl groups contain from 3 to about 20 ring members, while other such groups contain from 3 to about 15 ring members. In some examples, heterocyclyl groups include heterocyclyl groups containing 3 to 8 carbon atoms (C3-C8), 3 to 6 carbon atoms (C3-C6), 3 to 5 carbon atoms (C3-C5), 3 to 4 carbon atoms (C3-C4), or 6 to 8 carbon atoms (C6-C8). A heterocyclyl group designated as C2-heterocyclyl can be a 5-membered ring with 2 carbon atoms and 3 heteroatoms, a 6-membered ring with 2 carbon atoms and 4 heteroatoms, etc. Similarly, a C4-heterocyclyl can be a 5-membered ring with 1 heteroatom, a 6-membered ring with 2 heteroatoms, etc. The sum of the number of carbon atoms and the number of heteroatoms equals the total number of ring atoms. A heterocyclyl ring can also contain one or more double bonds. A heteroaryl ring is an example of a heterocyclyl group. The reference "heterocyclyl group" includes fused ring species, including fused aromatic and non-aromatic groups. Representative heterocyclyl groups include, but are not limited to, piperidinyl, piperazinyl, morpholinyl, furanyl, pyrrolidinyl, pyridinyl, pyrazinyl, pyrimidinyl, triazinyl, thiophenyl, tetrahydrofuranyl, pyrrolyl, oxazolyl, imidazolyl, triazyolyl, tetrazolyl, benzoxazolinyl, and benzimidazolinyl groups.

[0046] The term "alkoxy" as used herein refers to an oxygen atom linked to an alkyl group, including cycloalkyl groups as defined herein. Examples of linear alkoxy groups include, but are not limited to, methoxy, ethoxy, propoxy, butoxy, pentyloxy, hexyloxy, and the like. Examples of branched alkoxy groups include, but are not limited to, isopropoxy, sec-butoxy, tert-butoxy, isopentyloxy, isohexyloxy, and the like. Examples of cyclic alkoxy groups include, but are not limited to, cyclopropyloxy, cyclobutyloxy, cyclopentyloxy, cyclohexyloxy, and the like. An alkoxy group can contain from 1 to about 12-20 or about 12-40 carbon atoms bonded to an oxygen atom, and can further contain double or triple bonds and can also contain heteroatoms. For example, an allyloxy group is an alkoxy group within the meaning herein. A methoxyethoxy group is also an alkoxy group within the meaning herein, as is a methylenedioxy group when two adjacent atoms of the structure are replaced with said methylenedioxy group.

[0047] The term "amine" as used herein refers to primary, secondary, and tertiary amines, e.g., having the formula N(group)3, where each group can independently be H or other than H, e.g., alkyl, aryl, etc. Amines include, but are not limited to, alkyl amines, aryl amines, arylalkyl amines; dialkyl amines, diaryl amines, diaralkyl amines, heterocyclyl amines, etc., and ammonium ions.

[0048] The terms "halo," "halogen," or "halide" group as used herein, by themselves or as part of another substituent, mean, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom.

[0049] As used herein, the terms "salt" and "pharmaceutical acceptable salt" refer to derivatives of the disclosed compounds, in which the parent compound is modified by forming its acid or base salt. Examples of pharmaceutical acceptable salts include, but are not limited to, mineral or organic acid salts of basic groups such as amines; and alkali or organic salts of acidic groups such as carboxylic acids. Pharmaceutically acceptable salts include the conventional non-toxic salts or quaternary ammonium salts of the parent compound, for example, formed from non-toxic inorganic or organic acids. For example, such conventional non-toxic salts include those derived from inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, sulfamic acid, phosphoric acid, and nitric acid; and salts prepared from organic acids such as acetic acid, propionic acid, succinic acid, glycolic acid, stearic acid, lactic acid, malic acid, tartaric acid, citric acid, ascorbic acid, pamoic acid, maleic acid, hydroxymaleic acid, phenylacetic acid, glutamic acid, benzoic acid, salicylic acid, sulfanilic acid, 2-acetoxybenzoic acid, fumaric acid, toluenesulfonic acid, methanesulfonic acid, ethanedisulfonic acid, oxalic acid, and isethionic acid.

[0050] Pharmaceutically acceptable salts can be synthesized by conventional chemical methods from parent compounds that contain basic or acidic moieties.In some cases, such salts can be prepared by reacting the free acid or free base forms of these compounds with stoichiometric amounts of suitable base or acid in water or in an organic solvent or in the mixture of the two, generally preferred non-aqueous media such as ether, ethyl acetate, ethanol, isopropanol or acetonitrile.A list of suitable salts can be found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, the disclosure of which is incorporated herein by reference.

[0051] The term "solvate" refers to a compound or a salt thereof that further contains a stoichiometric or non-stoichiometric amount of solvent bound by non-covalent intermolecular forces. When the solvent is water, the solvate is a hydrate.

[0052] The term "prodrug" refers to a derivative of a compound that can be hydrolyzed, oxidized, or otherwise reacted under biological conditions (in vitro or in vivo) to provide an active compound, particularly a compound of the present invention. Examples of prodrugs include, but are not limited to, derivatives and metabolites of the compounds of the present invention that contain a biohydrolyzable moiety, such as biohydrolyzable amides, biohydrolyzable esters, biohydrolyzable carbamates, biohydrolyzable carbonates, biohydrolyzable ureides, and biohydrolyzable phosphate analogs. Specific prodrugs of a compound with a carboxyl functional group are the lower alkyl esters of the carboxylic acid. Carboxylic acid esters are also conveniently formed by esterification of any carboxylic acid moiety present on the molecule. Prodrugs can typically be prepared using well-known methods, such as those described in Burger's Medicinal Chemistry and Drug Discovery 6th ed. (Donald J. Abraham ed., 2001, Wiley) and Design and Application of Prodrugs (H. Bundgaard ed., 1985, Harwood Academic Publishers GmbH). EXAMPLES

[0053] The present invention may be better understood by reference to the following examples, which are provided by way of illustration only and are not intended to be limiting of the invention.

[0054] Synthesis Overview The retrosynthesis of calyspongiolide is outlined in Scheme 1. TIFF0007641933000029.tif149128

[0055] Late Sonogashira coupling of vinyl iodide 2 with both enantiomers of enyne 3 would provide access to both enantiomers of the proposed molecule. Macrolactones are constructed by macrolactonization of seco-acid 4, accessible by ring-opening of lactone 5. The "overcrowded" olefin of 5 would be formed via a modified Julia olefination using a sulfone derived from known diol 7 and an aldehyde obtained from known alcohol 6. Wittig olefination with an aldehyde derived from olefin 8 would provide enyne 3. Stereoselective Corey-Bakshi-Shibata (CBS) reduction of a hindered ketone derived from commercially available aldehyde 9 would provide both isomers.

[0056] As shown in Scheme 2, the synthesis of potash spongiolides begins with the known chiral alcohol 6, which is prepared in four steps from commercially available (S)-(+)-3-bromo-2-methyl-1-propanol. Treatment of the allylic alcohol 6 with acryloyl chloride and triethylamine afforded the diene 11, which was then subjected to ring-closing metathesis using Grubbs II catalyst to afford the α,β-unsaturated lactone 12. 1,4-addition using Me2CuLi2I stereoselectively delivered the methyl substituent to the bottom face of the molecule to afford the corresponding 4,6-anti-substituted lactone 13 as a single diastereomer ( 14 ) in 95% yield. 1 H NMR (determined). Treatment of the tert-butyldiphenylsilyl (TBDPS) ether with tetrabutylammonium fluoride (TBAF) smoothly afforded the corresponding alcohol, which upon oxidation with Dess-Martin periodinane DMP in the presence of NaHCO3 provided the aldehyde 14 in 90% yield. TIFF0007641933000030.tif97128

[0057] The known diol 7 was easily prepared in four steps from commercially available 2-butyne-1,4-diol as described by Crimmins et al. Selective Mitsunobu rearrangement with 1-phenyl-1H-tetrazole-5-thiol (PTSH, 16) followed by protection of the latent secondary alcohol provided the sulfide 17. See Scheme 3. Oxidation with m-chloroperbenzoic acid (m-CPBA) afforded the sulfone 18, which was ready for the improved Julia olefination reaction. Metallation with lithium hexamethyldisilazide (LHMDS) in dimethylformamide (DMF) at -60 °C, followed by addition of the aldehyde 14 afforded the sulfone 19 in 74% yield. 1 The desired trans-disubstituted olefin 5 was obtained with a trans / cis ratio of 34:1 as determined by 1 H NMR. TIFF0007641933000031.tif90128

[0058] The lactone 5 was reacted with N,O-dimethylhydroxylamine hydrochloride and iRing opening with PrMgCl in tetrahydrofuran (THF) at −20 °C provided the corresponding Weinreb amide (Scheme 4). Protection of the secondary alcohol as the tert-butyldimethylsilyl (TBS) ether with TBSCl and triethylamine in DMF or dichloromethane (DCM) afforded the TBS ether 19 in 85% yield over two steps. Reduction with diisobutylaluminum hydride (DIBAL-H) ​​followed by treatment with Ohira-Bestmann reagent and K2CO3 in MeOH afforded the carbon-enhanced alkyne 20. Alkylation of the terminal acetylene moiety by metallation with n-BuLi in THF at −78 °C, followed by treatment with ethyl chloroformate afforded the alkynyl ester 21. Selective removal of the triethylsilyl (TES) ether with pyridinium p-toluenesulfonate (PPTS) in methanol at 0 °C, followed by LiOH-mediated ester hydrolysis provided the corresponding seco acid. Yonemitsu's validation of the macrolactonization was highly successful, with no mixed anhydride formed first, but rather the seco acid added directly to 2,4,6-trichlorobenzoyl chloride, 4-dimethylaminopyridine (DMAP), and an amine base to provide the alkynyl lactone 22 in 67% yield over three steps. Reduction of the alkynyl moiety was achieved using quinoline-poisoned Lindlar catalyst under a hydrogen balloon. Removal of the TBS ether was ineffective even at elevated temperatures with tetra-n-butylammonium fluoride (TBAF). On the other hand, use of HF-pyridine was highly efficient, affording the corresponding alcohol. Chlorosulfonyl isocyanate was used to introduce the carbamate moiety in 80% yield over both steps. Removal of the para-methoxybenzyl (PMB) protecting group was achieved with 2,3-dichloro-5,6-dicyanobenzoquinone (DDQ) to provide the corresponding alcohol. This was oxidized using DMP and then subjected to Takai olefination to provide the vinyl iodide 2 in 42% yield over three steps. TIFF0007641933000032.tif158128

[0059] After protection of commercially available 2-bromo-3-hydroxybenzaldehyde (9) as the TBS phenol (Scheme 5), reverse prenylation with 3-methyl-2-butenylmagnesium chloride afforded alcohol 8 in 98% yield over two steps. Oxidation of the racemic alcohol with pyridinium chlorochromate (PCC) / SiO2 in DCM afforded the corresponding ketone. This was subjected to chiral reduction with Corey's (S)-Me-CBS catalyst to provide alcohol (S)-8 in 57% yield and 95% ee (as determined by chiral HPLC). Synthesis and X-ray crystallography of the 4-nitrobenzoate derivative confirmed the stereochemical outcome. The hindered alcohol was protected with triethylsilyl trifluoromethanesulfonate (TESOTf). Ozonolysis of the terminal olefin, including silyl deprotection, afforded a mixture of products. Nicolau's conditions for oxidative cleavage of the olefin provided the corresponding aldehyde, which was then subjected to Wittig olefination with commercially available phosphonium bromide 26 to give the corresponding TMS-protected enyne in a trans / cis ratio of 7:1. Global deprotection of all silyl protecting groups was achieved using TBAF to afford the enyne (S)-3 in 53% yield over three steps. Similarly, (R)-3 was prepared using the same chemistry, except that (R)-Me-CBS was used during the chiral reduction. TIFF0007641933000033.tif115128

[0060] Sonogashira coupling of vinyl iodide 2 with (S)-3 afforded the desired dienyne (S)-1 in 42% yield over three steps (Scheme 6). Similarly, coupling with the (R)-enantiomer afforded (R)-1 in 39% yield over three steps. TIFF0007641933000034.tif105128

[0061] The synthetic methods presented herein provide access to analogs of compounds of formula (I) and / or (II), such as compounds 28-39. TIFF0007641933000035.tif101128TIFF0007641933000036.tif188129

[0062] Synthesis of diene 11: To a clear solution of the alcohol (5.3783 g, 14.1 mmol) in DCM (100 mL) at 0 °C, EtN (9.8 mL, 70.3 mmol, 5 equiv.) and acryloyl chloride (2.3 mL, 28.3 mmol, 2 equiv.) were added sequentially. After 30 min, silica gel was added and the mixture was concentrated by rotary evaporation. The crude product was purified by column chromatography (3% EA / HX) to give 3.2532 g (53% yield) of the diene as a clear oil. R f = 0.4(5% EA / HX); TIFF0007641933000037.tif41128

[0063] Synthesis of a,b-unsaturated lactone 12: A deep purple solution of the diene (5.7564 g, 13.2 mmol) and Grubbs II catalyst (586.2 mg, 0.690 mmol, 5.2 mol %) in DCM (750 mL) was heated to reflux while covered with aluminum foil. After 14 h, the reaction was cleaved with ethyl vinyl ether (0.66 mL, 6.89 mmol, 0.5 equiv) and left stirring for 1 h. After concentration by rotary evaporation, column chromatographic purification (10% EA / HX) afforded 5.5139 g (96% yield) of the α,β-unsaturated lactone as a clear oil. R f = 0.2(10% EA / HX); TIFF0007641933000038.tif47128

[0064] Synthesis of lactone 13: To a tan suspension of CuI (3.1795 g, 16.7 mmol, 2.0 equiv) in Et2O (60 mL) was added MeLi (3.1 M DEM, 10.8 mL, 33.5 mmol, 4.0 equiv) slowly at 0 °C. After stirring for 30 min, the clear solution was cooled to -78 °C and a clear solution of the lactone (3.4087 g, 8.34 mmol) in Et2O (20 mL) was added slowly via cannula. The flask was warmed to -10 °C over 3 h and then quenched with saturated NH4Cl. The crude product was extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by flash chromatography (10% EA / HX) gave 3.3657 g (95% yield) of the lactone as a clear oil. R f = 0.3(15% EA / HX); TIFF0007641933000039.tif30133

[0065] Synthesis of aldehyde 14: To a clear solution of TBDPS ether (3.3779 g, 7.95 mmol) in THF (40 mL) was added TBAF (1 M THF, 16.7 mL, 16.7 mmol, 2 equiv.) slowly. After 45 min, the reaction was quenched with saturated NH4Cl. The crude product was extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by flash chromatography (50% EA / HX) gave 1.2741 g of the alcohol as a clear oil.

[0066] To a clear solution of the alcohol (1.2741 g, 6.84 mmol) in DCM (40 mL) at 0 °C, NaHCO3 (2.8733 g, 34.2 mmol, 5.0 equiv.) and DMP (5.8023 g, 13.7 mmol, 2.0 equiv.) were added in successive portions. After 10 min, the ice bath was removed and the reaction was stirred at room temperature for an additional 1.5 h. The reaction was quenched with a 1:1 solution of saturated NaHCO3 / Na2S2O3 solution and left to stir vigorously for 10 min. The crude product was extracted with DCM (x3), washed with brine, and dried over MgSO4. Purification by passing through a short silica plug (40% EA / HX) afforded 1.1286 mg (77% yield over two steps) of the aldehyde as a clear oil. R f = 0.4(50% EA / HX); TIFF0007641933000040.tif28128

[0067] Synthesis of sulfide 17: To a clear solution of diol (3.3165 g, 13.8 mmol), PPh3 (5.0855 g, 19.4 mmol, 1.4 equiv.) and PTSH (3.4498 g, 19.4 mmol, 1.4 equiv.) in THF (140 mL) was slowly added DIAD (3.8 mL, 19.3 mmol, 1.4 equiv.) at -20 °C. After 1 h, silica gel was added and the mixture was concentrated by rotary evaporation. The crude product was purified by flash chromatography (30% EA / HX) to give 4.9748 g of the sulfide as a clear oil.

[0068] To a clear solution of the alcohol (4.9748 g, 12.4 mmol) in DCM (100 mL) at 0 °C, Et3N (9.0 mL, 64.6 mmol, 5.2 equiv) and TESOTf (5.6 mL, 24.8 mmol, 2.0 equiv) were added successively. After 45 min, the reaction was quenched with saturated NaHCO3 and the flask was allowed to warm to room temperature. The crude product was extracted with DCM (x3), washed with brine, and dried over MgSO4. Purification by flash chromatography (5% EA / HX) afforded 5.6118 g (88% yield for two steps) of the sulfide as a clear oil. R f = 0.6(25% EA / HX); TIFF0007641933000041.tif47128

[0069] Synthesis of sulfone 18: To a clear solution of the sulfide (7.1321 g, 13.9 mmol) in DCM (70 mL) were added NaHCO3 (11.6666 g, 139 mmol, 10 equiv.) and m-CPBA (70 wt %, 17.1966 g, 69.8 mmol) in successive portions. After 13 h, the flask was cooled to 0 °C and carefully quenched with saturated NaHCO3. The crude product was extracted with DCM (×3), washed with saturated NaHCO3, brine, and dried over MgSO4. Purification by flash chromatography (5% EA / HX) afforded 7.5755 g (90% yield) of the sulfone as a clear oil. R f = 0.3(10% EA / HX); TIFF0007641933000042.tif47128

[0070] Synthesis of lactone 5: To a clear solution of the sulfone (2.3409 g, 4.28 mmol, 1.6 equiv) in DMF (20 mL) at -60 °C, LHMDS (1 M THF, 4.7 mL, 4.7 mmol, 1.8 equiv) was added slowly over 5 min. After 45 min, a clear solution of the aldehyde (481.7 mg, 2.62 mmol) in DMF (7 mL) was added slowly via cannula. After 1.5 h, the reaction was quenched with saturated NH4Cl and the flask was allowed to warm to room temperature. The crude product was diluted with H2O, extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by flash chromatography (10% EA / HX) afforded 976.6 mg (74% yield, 34:1 trans / cis) of the lactone as a clear oil. R f = 0.5(25% EA / HX); TIFF0007641933000043.tif66128

[0071] Synthesis of Weinreb amide 19: A cloudy mixture of lactone (939.0 mg, 1.86 mmol) and amine salt (371.5 mg, 3.81 mmol, 2.0 equiv) in THF (19 mL) was stirred at -20 °C for 1 h. i PrMgCl (2 M THF, 4.6 mL, 9.20 mmol, 5.0 equiv) was added slowly. After 45 min, the reaction was quenched with saturated NH4Cl and the flask was allowed to warm to room temperature. The crude product was diluted with H2O, extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by flash chromatography (30% EA / HX) gave 968.4 mg of the Weinreb amide as a clear oil.

[0072] To a clear solution of the alcohol (968.4 mg, 1.71 mmol) in DMF (17 mL) at 0 °C, Et3N (950 mL, 6.82 mmol, 4.0 equiv) and TBSCl (386.7 mg, 2.57 mmol, 1.5 equiv) were added successively. After 1.5 h, the reaction was extensively diluted with H2O. The crude product was extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by flash chromatography (10% EA / HX) gave 1.0709 g (92% yield) of the TBS ether as a clear oil. R f = 0.2(30% EA / HX); TIFF0007641933000044.tif60128

[0073] Synthesis of alkyne 20: To a clear solution of the aldehyde (378.6 mg, 0.557 mmol) in THF (5 mL) at -78 °C, DIBAL-H (1 M HX, 0.85 mL, 0.850 mmol, 1.5 equiv.) was added slowly. After 30 min, the reaction was quenched with several drops of MeOH, diluted with EA, quenched with sodium potassium tartrate, and the flask was allowed to warm to room temperature. After 1.5 h of vigorous stirring, the crude product was extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by flash chromatography (5% EA / HX) gave 344.9 mg (quantitative) of the aldehyde as a clear oil.

[0074] To a white mixture of the aldehyde (344.9 mg, 0.555 mmol) and K2CO3 (234.0 mg, 1.69 mmol, 3.0 equiv) in MeOH (6 mL) was added Ohira-Bestmann reagent (125 mL, 0.833 mmol, 1.5 equiv) slowly. After 2 h, the reaction was quenched with H2O. The crude product was extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by passing through a short silica plug (40% EA / HX) gave 322.9 mg (94% yield for two steps) of the aldehyde as a clear oil. R f= 0.5(5% EA / HX); TIFF0007641933000045.tif59128

[0075] Synthesis of alkynyl ester 21: To a clear solution of the alkyne (320.9 mg, 0.520 mmol) in THF (5 mL) was added n-BuLi (2.5 M HX, 250 mL, 0.625 mmol, 1.2 equiv) dropwise at -78 °C. After 20 min, a neat solution of ethyl chloroformate (100 mL, 1.05 mmol, 2.0 equiv) was added dropwise. The reaction was quenched with saturated NH4Cl after 1 h and the flask was allowed to warm to room temperature. The crude product was extracted with EA (x3), washed with brine and dried over Na2SO4. Purification by flash chromatography (3% EA / HX) afforded 347.7 mg (97% yield) of the alkyne as a clear oil. R f = 0.3(5% EA / HX); TIFF0007641933000046.tif72128

[0076] Synthesis of alkynyl lactone 22: To a clear solution of TES ether (346.6 mg, 0.503 mmol) in EtOH (5 mL) was added PPTS (25.4 mg, 0.101 mmol, 0.2 equiv) in one portion at 0 °C. After 17 h, the reaction was quenched with saturated NaHCO3 and the flask was allowed to warm to room temperature. The crude product was extracted with EA (x3), washed with brine and dried over Na2SO4.

[0077] To a clear solution of the alkynyl ester in THF / HO (1:1, 5 mL), LiOH . H2O (211.3 mg, 5.04 mmol, 10 equiv) was added in one portion. After 18 h, the reaction was quenched with 1N HCl and the crude product was extracted with EA (x3), washed with brine and dried over Na2SO4.

[0078] To a cloudy mixture of 2,4,6-trichlorobenzoyl chloride (105 mL, 0.672 mmol, 1.3 equiv), DIPEA (440 mL, 2.53 mmol, 5.0 equiv) and DMAP (30.9 mg, 0.253 mmol, 0.5 equiv) in PhMe (90 mL) was slowly added a clear solution of seco acid in PhMe (10 mL) via cannula. After 2 h, the reaction was quenched with saturated NaHCO3. The crude product was extracted with EA (x3), washed with brine and dried over Na2SO4. Purification by flash chromatography (3% EA / HX) afforded 178.4 mg (67% yield for 3 steps) of the alkynyl lactone as a clear oil. R f = 0.2(5% EA / HX); TIFF0007641933000047.tif66128

[0079] Synthesis of α,β-unsaturated lactone 23: A black mixture of alkynyl lactone (68.0 mg, 0.129 mmol), quinoline (18 mL, 0.152 mmol, 1.2 equiv.) and Lindlar's catalyst (14.1 mg) in EA / 1-hexene (1:1, 3 mL) was flushed with Ar (×5), H2 (×5) and left stirring vigorously under a H2 balloon. After 3 h, the reaction mixture was passed through Celite and washed thoroughly with EA. Purification by flash chromatography (3% EA / HX) afforded 67.4 mg (99% yield) of the a,b-unsaturated lactone as a clear oil. R f = 0.2(5% EA / HX); TIFF0007641933000048.tif71128

[0080] Synthesis of carbamate 24: HF .py (70%, 200 mL) was added dropwise to a clear solution of TBS ether (56.5 mg, 0.106 mmol) in MeOH (2 mL) in a plastic vial at 0 °C. After 4 h, the reaction was carefully quenched with saturated NaHCO3 and the vial was allowed to warm to room temperature. The crude product was extracted with EA (x3), washed with saturated NaHCO3, brine and dried over Na2SO4. Purification by flash chromatography (20% EA / HX) gave 41.8 mg of the alcohol as a clear oil.

[0081] To a clear solution of the alcohol (41.8 mg, 0.100 mmol) in DCM (2 mL) was added neat chlorosulfonyl isocyanate (11 mL, 0.126 mmol, 1.3 equiv) dropwise at 0 °C. After 20 min, the reaction was slowly quenched with THF / H2O (4:1, 1 mL) and the flask was allowed to warm to room temperature. After 2 h of vigorous stirring, the reaction was quenched with saturated NaHCO3. The crude product was extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by flash chromatography (35% EA / HX) afforded 39.2 mg (80% yield for two steps) of the carbamate as a clear oil. R f = 0.1(35% EA / HX); TIFF0007641933000049.tif60128

[0082] Synthesis of vinyl iodide 2: To a clear / yellow biphasic mixture of PMB ether (36.0 mg, 0.078 mmol) in DCM / pH 7.0 phosphate buffer (1:1, 1.5 mL) was added DDQ (39.0 mg, 0.172 mmol, 2.2 equiv). After 1 h, additional DDQ was added (17.0 mg, 0.075 mmol, 1.0 equiv). After 3 h, the reaction was quenched and the crude product was extracted with EA (x3), washed with saturated NaHCO3, brine, and dried over Na2SO4. Purification by flash chromatography (75% EA / HX) gave 20.8 mg of the alcohol as a clear oil.

[0083] To a clear solution of the alcohol (20.8 mg, 0.061 mmol) in DCM (2 mL) at 0 °C were added NaHCO3 (25.7 mg, 0.306 mmol, 5.0 equiv.) and DMP (51.7 mg, 0.122 mmol, 2.0 equiv.) successively. After addition, the ice bath was removed and the reaction was left stirring for 1.5 h and then quenched with 3 mL of Na2S2O3 / sat. NaHCO3 (1:1). The crude product was extracted with EA (x3), washed with brine and dried over Na2SO4. Purification by passing through a short silica plug (50% EA / HX) gave 13.6 mg of the aldehyde as a white solid.

[0084] A yellow solution of the aldehyde (13.6 mg, 0.040 mmol) and CHI3 (47.2 mg, 0.120 mmol, 3.0 equiv) in THF (1 mL) was added via cannula to a green suspension of CrCl2 (49.3 mg, 0.401 mmol, 10.0 equiv) in THF (1 mL). After 2 h, the reaction was quenched with H2O and the crude product was extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by passing through a short silica plug (40% EA / HX) afforded 15.2 mg (42% yield over three steps) of the vinyl iodide as a white solid. R f = 0.2(25% EA / HX); TIFF0007641933000050.tif41128; further spectroscopic data was not collected due to concerns about decomposition.

[0085] Synthesis of alcohol 8: To a grey mixture of phenol (2.0692 g, 10.3 mmol) in DCM (50 mL) at 0 °C, DIPEA (3.5 mL, 20.1 mmol, 2.0 equiv) and TBSCl (1.9755 g, 13.1 mmol, 1.3 equiv) were added successively. After 4 h, the yellow solution was quenched with saturated NH4Cl. The crude product was extracted with EA (x3), washed with brine and dried over Na2SO4. Purification by flash chromatography (3% EA / HX) gave 3.1808 g of TBS phenol as a clear oil.

[0086] To a clear solution of the aldehyde (2.0692 g, 6.6 mmol) in THF (35 mL) was slowly added a grey solution of 3-methyl-2-butenylmagnesium chloride (1 M THF, 8.0 mL, 8.0 mmol, 1.2 equiv) at 0 °C. After 30 min, the reaction was quenched with saturated NH4Cl. The crude product was extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by flash chromatography (3% EA / HX) afforded 3.8880 g (98% yield for two steps) of the alcohol as a clear oil. R f = 0.3(5% EA / HX); TIFF0007641933000051.tif35128

[0087] Synthesis of alcohol (S)-8: To a cloudy suspension of the alcohol (2.4435 g, 6.34 mmol) and SiO2 (2.8888 g) in DCM (32 mL) was added PCC (2.0503 g, 9.51 mmol, 1.5 equiv) in small portions. After 12 h, the reaction was filtered through Celite and the filter cake was washed with DCM. Purification by flash chromatography (3% EA / HX) gave 2.2594 g of the ketone as a clear oil.

[0088] To a pale yellow solution of the ketone (2.2594 g, 5.89 mmol) and (S)-Me-CBS (1.6325 g, 5.89 mmol, 1.0 equiv) in PhMe (60 mL) was added BMS (1.1 mL, 11.6 mmol, 2.0 equiv) dropwise at 0 °C. After 2 h, the reaction was quenched with MeOH dropwise, the flask was allowed to warm to room temperature, and concentrated by rotary evaporation. Purification by flash chromatography (3% EA / HX) afforded 1.2955 g (53% yield over two steps) of the alcohol as a clear oil. TIFF0007641933000052.tif5128 Chiral HPLC: Chiralpak IC3 (250×4.6 mm), 5% IPA / HX, flow rate = 0.5 mL / min, T = 20°C, UV = 254 nm, R t Major = 7.9 minutes, R t Vice = 9.2 minutes.

[0089] Synthesis of alcohol (R)-8: Prepared in the same manner as alcohol (S)-8 above using (R)-Me-CBS catalyst. TIFF0007641933000053.tif5128

[0090] Synthesis of benzoate (S)-27: To a clear solution of the alcohol (32.0 mg, 0.083 mmol) in DCM (2 mL) were added Et3N (35 mL, 0.251 mmol, 3.0 equiv), 4-nitrobenzoyl chloride (24.5 mg, 0.132 mmol, 1.6 equiv) and DMAP (2.0 mg, 0.016 mmol, 0.2 equiv) successively. After 3 h, the reaction was quenched with saturated NaHCO3. The crude product was extracted with DCM (x3), washed with brine and dried over MgSO4. Purification by flash chromatography (3% EA / HX) afforded 42.6 mg (96% yield) of the benzoate ester as an opaque solid. R f = 0.4(5% EA / HX); TIFF0007641933000054.tif47128

[0091] Synthesis of TES ether (S)-25: To a clear solution of the alcohol (153.7 mg, 0.399 mmol) in DCM (3 mL) at 0 °C, Et3N (170 mL, 1.22 mmol, 3.1 equiv) and TESOTf (135 mL, 0.597 mmol, 1.5 equiv) were added successively. After 30 min, the reaction was quenched with saturated NaHCO3 and the flask was allowed to warm to room temperature. The crude product was extracted with DCM (x3), washed with brine, and dried over MgSO4. Purification by flash chromatography (2% EA / HX) afforded 191.3 mg (96% yield) of the TES ether as a clear oil. R f = 0.7(5% EA / HX); TIFF0007641933000055.tif41129

[0092] Synthesis of TES ether (R)-25: Prepared in the same manner as TES ether (S)-25 above. TIFF0007641933000056.tif5128

[0093] Synthesis of enyne (S)-3: To a clear solution of olefin (238.9 mg, 0.478 mmol) in acetone / HO (5 mL) were added 2,6-lutidine (110 mL, 0.944 mmol, 2.0 equiv), NMO (115.2 mg, 0.983 mmol, 2.1 equiv) and OsO4 (4% in HO, 0.6 mL, 0.094 mmol, 0.2 equiv) successively. The yellow solution was left stirring overnight covered with aluminum foil. After 12 h, PhI(OAc)2 (240.2 mg, 0.746 mmol, 1.6 equiv) was added and the reaction was left stirring for 30 min. The reaction was quenched with Na2S2O3 and left stirring vigorously for 15 min. The crude product was extracted with EA (x3), washed with brine and dried over Na2SO4. Purification by flash chromatography (3% EA / HX) gave 224.0 mg of the aldehyde as a clear oil.

[0094] To a pale yellow suspension of phosphonium bromide (822.0 mg, 1.81 mmol, 4.0 equiv) in THF (10 mL) at -78 °C was slowly added LHMDS (1 M THF, 1.8 mL, 1.80 mmol, 4.0 equiv). After 10 min, the flask was warmed to -40 °C for an additional 30 min and then cooled back to -78 °C. A clear solution of aldehyde (224.0 mg, 0.447 mmol) in THF (5 mL) was then slowly added via cannula. The flask was then slowly warmed to room temperature and left stirring overnight. After 12 h, the reaction was quenched with saturated NH4Cl. The crude product was extracted with EA (x3), washed with brine and dried over Na2SO4. Purification by flash chromatography (3% EA / HX) gave 1 272.2 mg of TMS-enyne was obtained as a clear oil with a trans / cis ratio of 7:1 as determined by 1 H NMR.

[0095] To a clear solution of the tri-silyl ether (272.2 mg, 0.478 mmol) in THF (5 mL) was slowly added TBAF (1 M THF, 2.4 mL, 2.40 mmol, 5.0 equiv). After 20 h, the reaction was quenched with saturated NH4Cl. The crude product was extracted with EA (x3), washed with brine, and dried over Na2SO4. Purification by flash chromatography (15% EA / HX) afforded 72.0 mg (51% yield over three steps) of the enyne as a clear oil. R f = 0.2(15% EA / HX); TIFF0007641933000057.tif35128

[0096] Synthesis of enyne (R)-3: Prepared in the same manner as TES ether (S)-3 above. TIFF0007641933000058.tif5128

[0097] Synthesis of dienyne (S)-1: To a clear solution of vinyl iodide (8.6 mg, 0.019 mmol) and enyne (8.4 mg, 0.028 mmol, 1.5 equiv) in degassed PhMe (3 mL), pyrrolidine (3 mL, 0.036 mmol, 1.9 equiv) and CuI (0.7 mg, 0.004 mmol, 0.2 equiv) were added sequentially to give a dark red solution. After stirring for 5 min, Pd(PPh3)4 (2.2 mg, 0.002 mmol, 0.1 equiv) was added to give a pale yellow solution. After 45 min, the reaction was passed through a short plug of silica and eluted with Et2O. Purification by flash chromatography (25% EA / HX) afforded 9.2 mg (79% yield for three steps) of the potash spongiolide, (S)-1, as a clear oil. R f = 0.14(40% EA / HX); TIFF0007641933000059.tif72128

[0098] Synthesis of dienyne (R)-1: To a clear solution of vinyl iodide (3.2 mg, 0.007 mmol) and enyne (3.1 mg, 0.011 mmol, 1.6 equiv) in degassed PhMe (2 mL), pyrrolidine (1.5 mL, 0.018 mmol, 2.6 equiv) and CuI (0.7 mg, 0.004 mmol, 0.6 equiv) were added sequentially to give a dark red solution. After stirring for 5 min, Pd(PPh3)4 (0.9 mg, 0.001 mmol, 0.1 equiv) was added to give a pale yellow solution. After 45 min, the reaction was passed through a short plug of silica and eluted with Et2O. Purification by flash chromatography (25% EA / HX) gave 3.6 mg (82% yield) of potash spongiolide, (R)-1, as a clear oil. R f = 0.14(40% EA / HX); TIFF0007641933000060.tif71128

[0099] The present invention provides the following examples, the numbering of which should not be construed as indicating any level of importance.

[0100] Example 1 is a compound of formula (IV): The compound of formula (V): contacting the compound of formula (I) with the compound of formula (I). Formula (III): 1. A method for making a compound of TIFF0007641933000063.tif37128 or a pharma- ceutically acceptable salt, polymorph, prodrug, solvate, or clathrate thereof, comprising: During the ceremony, each dashed bond independently represents a single bond or a double bond, and when the dashed bond represents a double bond, the double bond can have an E or Z configuration; 1 is H, alkyl, R 2 -(CH2) q -X 3 -, R 2 -(CH2) q -C(O)NR 6 (CH2) q -X 3 -, OR 6 , or N(R 6 )2, where R 6 each independently represents H, alkyl, aryl, alkaryl, or arylalkyl, and each q independently represents an integer of 0 to 9; X 1 , O, NR 6 , or S;X 2 , O, NR 6 , or S;R 2 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 )2, where p is an integer from 0 to 2 and m is an integer from 1 to 10; X 3 , O, NR 6 , S(O) p , or C(R 6 )2;X 4O, NR 6 , or S, or X 4 and X 4 If there is a single bond between the carbon atom to which X is bonded, 4 is R 2 R 3 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 )2;R 4 is H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , N(R 6 )2, or X 1 C(O)(CH) q R 6 and R 5 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 ) 2, and n is an integer from 0 to 4.

[0101] Example 2 relates to the process of Example 1, wherein m is 3 and / or n is 2.

[0102] Example 3 illustrates a compound of formula (III) having the formula: The compound of formula (IV) is a compound of formula (X): This relates to the methods of Examples 1 and 2, which are compounds of TIFF0007641933000065.tif36128.

[0103] Example 4 illustrates a compound of formula (III) having the formula: TIFF0007641933000066.tif31128, the compound of formula (V) is a compound of formula (XI): This relates to the method of Example 1, which is the compound of TIFF0007641933000067.tif30128.

[0104] Example 5 illustrates a compound of formula (III) having the formula: This relates to the method of Example 1, which is the compound of TIFF0007641933000068.tif33128.

[0105] Example 6 illustrates a compound of formula (III) having the formula: This relates to the method of Example 1, which is the compound of TIFF0007641933000069.tif33128.

[0106] Example 7 illustrates a compound of formula (III) having the formula: This relates to the method described in Example 1, which is the compound of TIFF0007641933000070.tif33128.

[0107] Example 8 is X 1 , X 2 , X 3 , and X 4 and each is O.

[0108] Example 9 relates to the processes of Examples 1-8, where each dashed bond represents a double bond in the configuration shown.

[0109] Example 10 is R 1 N(R 6 )2, and R 6 each independently represents H, alkyl, aryl, alkaryl, or arylalkyl.

[0110] Example 11 is R 2 is each independently C1 to C6 alkyl.

[0111] Example 12 is R 5 each independently represents halo or OR 6 This relates to the methods of Examples 1 to 11,

[0112] Example 13 is R 3 is each independently C1 to C6 alkyl.

[0113] Example 14 is R 4 OR 6 The present invention relates to the methods described in Examples 1 to 13, wherein

[0114] Example 15 illustrates a compound of formula (III) having formula (I): This relates to the methods of Examples 1 to 14, which are compounds of TIFF0007641933000071.tif37128.

[0115] Example 16 shows a compound of formula (III): or a pharma- ceutically acceptable salt, polymorph, prodrug, solvate, or clathrate thereof, wherein each dashed bond independently represents a single bond or a double bond, and when the dashed bond represents a double bond, the double bond can have an E or Z configuration; 1 is H, alkyl, R 2 -(CH2) q -X 3 -, R 2 -(CH2) q -C(O)NR 6 (CH2) q -X 3 -, OR 6 , or N(R 6 )2, where R 6 each independently represents H, alkyl, aryl, alkaryl, or arylalkyl, and each q independently represents an integer of 0 to 9; X 1 , O, NR 6 , or S;X 2 , O, NR 6 , or S;R 2 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6)2, where p is an integer from 0 to 2 and m is an integer from 1 to 10; X 3 , O, NR 6 , S(O) p , or C(R 6 )2;X 4 O, NR 6 , or S, or X 4 and X 4 If there is a single bond between the carbon atom to which X is bonded, 4 is R 2 R 3 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 )2;R 4 is H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , N(R 6 )2, or X 1 C(O)(CH) q R 6 and R 5 each independently represents H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR 6 , S(O) p R 6 , or N(R 6 ) 2, n is an integer from 0 to 4, and the compound of formula (III) is a compound of formulas (I) and (II): Not the compound TIFF0007641933000073.tif82128.

[0116] Example 17 relates to a pharmaceutical composition comprising the compound of Example 16 and a pharma- ceutically acceptable carrier.

[0117] Example 18 relates to a method for treating cancer, comprising administering to a subject in need thereof a therapeutically effective amount of one or more compounds of Example 16 or a pharmaceutical composition comprising one or more compounds of Example 16.

[0118] Example 19 relates to the compound of Example 16 for use as a medicament for the treatment of a patient in need of palliation from cancer.

Claims

[Claim 1] Formula (IV): with a compound of formula (V): with a compound of Formula (III): or a pharma- ceutically acceptable salt thereof, comprising the steps of: During the ceremony, R 2 are each independently H or alkyl; R 3 are each independently alkyl; R 4 is H, alkyl, or OR 6 and R 5 each independently represents H, halo, alkyl, or OR 6 and The method wherein each R 6 is independently H, or alkyl.

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