Broadly neutralizing anti-HIV antibodies

Abstract

To provide broad and potent antibodies against Human Immunodeficiency Virus (HIV), and therapeutic methods using the antibodies.SOLUTION: The present invention provides a pharmaceutical composition for use in preventing or treating an HIV infection or an HIV-related disease, comprising (i) a first anti-HIV antibody or an antigen binding portion thereof and (ii) a second anti-HIV antibody or an antigen binding portion thereof. The second anti-HIV antibody is an anti-CD4 binding site (CD4bs) antibody.SELECTED DRAWING: None

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority under 35 U.S.C. §119(e) to U.S. Provisional Application No. 61 / 715,642, filed October 18, 2012, which is hereby incorporated by reference in its entirety.

[0002] STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT The invention disclosed herein was made, at least in part, with Government support under Grant No. P01 AI081677 from the National Institutes of Health. Accordingly, the United States Government has certain rights in this invention.

[0003] The present invention relates to broad spectrum and potent antibodies against the Human Immunodeficiency Virus ("HIV"). [Background technology]

[0004] HIV is a condition in humans characterized by clinical features including a wasting syndrome, central nervous system degeneration and profound immunosuppression that leads to acquired immune deficiency syndrome (AIDS) and life-threatening opportunistic infections and malignancies. Since its discovery in 1981, HIV type 1 (HIV-1) has caused at least 25 million deaths worldwide. Even if HIV infections decline by 2.5% each year, it is projected that 20-60 million people will become infected over the next 20 years. There is a need for therapeutic agents and methods for the treatment or suppression of HIV infection.

[0005] Some HIV-infected individuals display broadly neutralizing IgG antibodies in their serum. However, little is known about the specificity and activity of these antibodies, despite their potential importance in designing effective vaccines. In animal models, passive transfer of neutralizing antibodies can contribute to protection against viral challenge. Neutralizing antibody responses can also be expressed in HIV-infected individuals, but the detailed composition of the serum response has not yet been fully characterized. Summary of the Invention

[0006] The present invention relates to a novel category of broadly neutralizing anti-HIV antibodies, the consensus heavy and light chain amino acid sequences of which are listed below and shown in Figures 3a and 3b: QVQLQESGPGLVKPSETLSLTCSVSGX 1 SX 2 X 3 DX 4 YWSWIRQSPGKGLEWIGYVHDSGDTNYNPSLKSRVX 5 X 6 SLDTSKNQVSLKLX 7 X 8 VTAADSAX 9 YYCARAX 10 HGX 11 RIYGIVAFGEX 12 FTYFYMDVWGKGTTVTVSS (SEQ ID NO: 1) SX 1 VRPQPPSLSVAPGETARIX 2 CGEX 3 SLGSRAVQWYQQRPGQAPSLIIYNNQDRPSGIPERFSGSPDX 4 X 5 FGTTATLTITX 6 VEAGDEADYYCHIWDSRX 7 PTX 8 WVFGGGTTLTVL (sequence number 2).

[0007] In the sequences of SEQ ID NO: 1 or 2, each "X" may be any amino acid residue or may be no amino acid. Preferably, each X may be the residue at the corresponding position in the clonal variants 10-259, 10-303, 10-410, 10-847, 10-996, 10-1074, 10-1121, 10-1130, 10-1146, 10-1341 and 10-1369 as shown in Figures 3a and 3b, and in the artificially modified version of the 10-1074 antibody, 10-1074GM.

[0008] Thus, one aspect of the invention features an isolated anti-HIV antibody, or antigen-binding portion thereof, having at least one complementarity determining region (CDR) having a sequence selected from the group consisting of SEQ ID NOs: 33-38, with the proviso that said antibody is not antibody PGT-121, 122 or 123. SEQ ID NOs: 33-38 refer to the sequences of heavy chain CDRs (CDRH) 1-3 and light chain CDRs (CDRL) 1-3 according to the Kabat system as shown in Figures 3a and 3b. In one embodiment, said CDRs can contain sequences selected from the group consisting of SEQ ID NOs: 39-104, i.e., CDR sequences according to the KABAT system as shown in Table 1 below. Alternatively, said CDRs can contain sequences selected from the CDR sequences of their corresponding antibodies according to the IMGT system as shown in Table 1 below.

[0009] In one embodiment, the isolated anti-HIV antibody, or antigen-binding portion thereof, comprises a heavy chain variable region comprising CDRH1, CDRH2 and CDRH3, wherein the CDRH1, CDRH2 and CDRH3 comprise the respective sequences of SEQ ID NOs: 33 to 35. The CDRH1, CDRH2 and CDRH3 can also comprise the respective sequences of a CDRH set selected from the group consisting of SEQ ID NOs: 39 to 41, 45 to 47, 51 to 53, 57 to 59, 63 to 65, 69 to 71, 75 to 77, 81 to 83, 87 to 89, 93 to 95, 99 to 101, and 131 to 133. Alternatively, the CDRHs can comprise the respective sequences selected from the CDR sequences of their corresponding antibodies according to the IMGT system as shown in Table 1 below.

[0010] In another embodiment, the isolated anti-HIV antibody, or antigen-binding portion thereof, comprises a light chain variable region comprising CDRL1, CDRL2 and CDRL3, wherein the CDRL1, CDRL2 and CDRL3 comprise the respective sequences of SEQ ID NOs: 36 to 38. For example, the CDRL1, CDRL2 and CDRL3 can comprise the respective sequences of a CDRL set selected from the group consisting of SEQ ID NOs: 42 to 44, SEQ ID NOs: 48 to 50, SEQ ID NOs: 54 to 56, SEQ ID NOs: 60 to 62, SEQ ID NOs: 66 to 68, SEQ ID NOs: 72 to 74, SEQ ID NOs: 78 to 80, SEQ ID NOs: 84 to 86, SEQ ID NOs: 90 to 92, SEQ ID NOs: 96 to 98, SEQ ID NOs: 102 to 104, and SEQ ID NOs: 134 to 136. Alternatively, the CDRLs can comprise the respective sequences selected from the CDR sequences of their corresponding antibodies according to the IMGT system as set forth in Table 1 below.

[0011] In yet another embodiment, the isolated anti-HIV antibody, or antigen-binding portion thereof, described above, comprises (i) a heavy chain variable region comprising CDRH1, CDRH2 and CDRH3, and (ii) a light chain variable region comprising CDRL1, CDRL2 and CDRL3. The CDRH1, CDRH2, CDRH3, CDRL1, CDRL2 and CDRL3 can comprise respective sequences of a CDR set selected from the group consisting of SEQ ID NOs: 39-44, 45-50, 51-56, 57-62, 63-68, 69-74, 75-79, 81-86, 87-92, 93-98, 99-104 and 131-136. Alternatively, the CDRHs and CDRLs can contain respective sequences selected from the CDR sequences of their corresponding antibodies according to the IMGT system as shown in Table 1 below.

[0012] In further embodiments, the isolated anti-HIV antibody, or antigen-binding portion thereof, contains one or both of (i) a heavy chain having the consensus amino acid sequence of SEQ ID NO: 1 and (ii) a light chain having the consensus amino acid sequence of SEQ ID NO: 2. The heavy chain can contain a sequence selected from the group consisting of SEQ ID NOs: 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, and 129, and the light chain can contain a sequence selected from the group consisting of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, and 130. For example, the heavy chain and the light chain can include the sequences of SEQ ID NOs: 3-4, SEQ ID NOs: 5-6, SEQ ID NOs: 7-8, SEQ ID NOs: 9-10, SEQ ID NOs: 11-12, SEQ ID NOs: 13-14, SEQ ID NOs: 15-16, SEQ ID NOs: 17-18, SEQ ID NOs: 19-20, SEQ ID NOs: 21-22, SEQ ID NOs: 23-24, and 129-130, respectively.

[0013] In a preferred embodiment, the isolated anti-HIV antibody is one selected from the group consisting of 10-259, 10-303, 10-410, 10-847, 10-996, 10-1074, 10-1074GM, 10-1121, 10-1130, 10-1146, 10-1341, and 10-1369. Their corresponding heavy chain variable regions, light chain variable regions, CDRH1-3, and CDRL1-3 are shown in Figures 3a and 3b. In a further preferred embodiment, the isolated anti-HIV antibody is one selected from the group consisting of 10-1074-like antibodies, i.e., 10-847, 10-996, 10-1074, 10-1074GM, 10-1146, and 10-1341. This group of antibodies is more potent than PGT121 in neutralizing current viruses. The antibodies discussed above can be human, humanized, or chimeric.

[0014] In a second aspect, the invention provides an isolated nucleic acid having a sequence encoding the CDRs, heavy chain variable region, or light chain variable region of an anti-HIV antibody, or antigen-binding portion thereof, discussed above. Also featured are vectors containing the nucleic acid, and cultured cells containing the vector.

[0015] The nucleic acid, vector, and cultured cells can be used in a method for making an anti-HIV antibody or a fragment thereof. This method includes, among others, the step of obtaining the above-described cultured cells, the step of culturing the cells in a medium under conditions that allow expression of the polypeptide encoded by the vector and construction of the antibody or a fragment thereof, and the step of purifying the antibody or fragment from the cultured cells or the medium of the cells.

[0016] In a third aspect, the invention features a pharmaceutical composition comprising (i) at least one of the above-described anti-HIV antibodies, or an antigen-binding portion thereof, and (ii) a pharmaceutically acceptable carrier.

[0017] In a fourth aspect, the invention provides a method for preventing or treating HIV infection or an HIV-related disease. This method includes, among others, the step of identifying a patient in need of such prevention or treatment; and the step of administering to the patient a first therapeutic agent containing a therapeutically effective amount of at least one of the above-described anti-HIV antibodies, or an antigen-binding portion thereof. The method can further include the step of administering a second therapeutic agent, such as an antiviral drug.

[0018] In a fifth aspect, the invention provides a kit having a pharmaceutically acceptable dosage unit of a pharmaceutically effective amount of at least one of the above-described isolated anti-HIV antibodies, or an antigen-binding portion thereof, and a pharmaceutically acceptable dosage unit of a pharmaceutically effective amount of an anti-HIV drug. The two pharmaceutically acceptable dosage units can optionally be in the form of a single pharmaceutically acceptable dosage unit. Exemplary anti-HIV drugs can be selected from the group consisting of non-nucleoside reverse transcriptase inhibitors, protease inhibitors, entry or fusion inhibitors, and integrase inhibitors.

[0019] In a sixth aspect, the present invention provides a kit for the diagnosis, prognosis or treatment monitoring of HIV infection in a subject. The kit contains one or more detection reagents that specifically bind to anti-HIV neutralizing antibodies in a biological sample from the subject. The kit may further include reagents for performing PCR or mass spectrometry.

[0020] The details of one or more embodiments of the invention are set forth in the description below. Other features, objects, and advantages of the invention will be apparent from the description and the claims. [Brief description of the drawings]

[0021] [Figure 1-1] Neutralizing activity of PGT121-like and 10-1074-like mutants. (A) Heatmap comparing neutralizing potency of PGT121-like and 10-1074-like antibodies in the TZM-bl assay. Darker colors = stronger neutralization; white = no neutralization. (B) Correlation between the average IC80 against 9 viruses (y-axis) and the apparent KD values ​​for binding to gp120 and gp140 (x-axis). [Figure 1-2] Neutralizing activity of PGT121-like and 10-1074-like mutants. (C) Graph comparing neutralization breadth and potency of PGT121, 10-996 and 10-1074 antibodies in the TZM-bl assay against an extended panel of 119 viruses. The y-axis shows the cumulative frequency of IC50 values ​​up to the concentration shown on the x-axis. The spider web graph (top left corner) shows the frequency distribution of neutralized viruses by HIV-1 clade. (D) Dot plot showing molar neutralization rate (MNR; IC50 concentration ratio of Fab to IgG). The horizontal bar represents the average IC50 across all viruses. [Figure 1-3]Figure showing the neutralizing activities of the PGT121-like and 10-1074-like variants. (E) Bar graph comparing the neutralizing potencies of PGT121 (dark gray) and 10-1074 (light gray) against viruses isolated from past (Hist.) and current (Cont.) antibody seroconverters. ns, not significant; **, p < 0.005. Fold difference between the median IC50s for neutralization of current viruses by PGT121 and 10-1074 is shown. [Diagram 2] Figure showing the binding and neutralizing activities of the PGT121GM and 10-1074GM mutant antibodies. (A) Bar graph comparing the apparent KD values for the binding of the 10-1074, PGT121, PGT121GM, and 10-1074GM antibodies to gp120 and gp140. Error bars indicate SEM of KD values obtained from three independent experiments. Fold difference between the KD values of the “wild-type” versus “glycomutant” antibodies is shown. (B) Bar graph comparing the binding of the glycan (Figure 7A) by PGT121 and 10-1074 with the binding by the mutant antibodies (PGT121GM and 10-1074GM). The numerical score for binding is measured as the fluorescence intensity (average of 2 replicate spots) for probes aligned at 5 fmol per spot. (C) Coverage graph comparing the neutralization breadth and potency of the PGT121, PGT121GM, 10-1074, and 10-1074GM antibodies in the TZM-bl assay against a panel of 40 viruses. [Figure 3-1]Figure 1 shows sequence alignment of PGT121 and 10-1074 clonal variants. (A) Amino acid alignment of the heavy chain (IgH) of PGT121-like and 10-1074-like antibodies and the predicted germline (GL) VH for all clonal variants. Amino acid numbering based on the crystal structure, framework (FWR) and complementarity determining regions (CDRs) as defined by Kabat (.J Exp Med 132(2):211-250) and IMGT (Nucleic Acids Res 37(Database issue):D1006-1012) is shown. Color shading indicates acidic (red), basic (blue) and tyrosine (green) amino acids. [Figure 3-2] Sequence alignment of PGT121 and 10-1074 clone variants (B) Same as A but for the light chain (IgL). [Figure 4] Figure 1 shows the binding affinity of PGT121 and 10-1074 clonal variants. (A) Binding affinity of PGT121 IgG antibody variants interacting with YU-2 gp140 and gp120 ligands as measured by surface plasmon resonance (SPR). M, mol / L; s, seconds; RU, response units; / , no binding detected. Chi2 values ​​(Χ2) < 10 indicate that the 1:1 binding model used to fit the curves adequately describes the experimental data. The equilibrium and rate constants shown are considered "apparent" constants to account for avidity effects resulting from bivalent binding of IgG. (B) Dot plots showing association (ka) and dissociation (kd) rate constants for PGT121-like (shaded in blue) and 10-1074-like (shaded in green). (C) Linear regression graph comparing the ka and kd values ​​for their binding to gp120 and gp140 (x-axis) of IgG antibodies against their neutralizing potency (average IC80 values) (y-axis) against the nine viruses shown in Table 4. [Figure 5-1]Figure 1. Binding of PGT121 mutants to gp120 "core" protein, gp120GD324-5AA mutant and linear gp120V3 peptide. (A) ELISA-based binding analysis of PGT121-like and 10-1074-like antibodies to HXB2 gp120 core and 2CC-core proteins compared to intact YU-2 gp120. The x-axis indicates the antibody concentration (M) required to obtain the ELISA value (OD405nm) shown on the y-axis. Anti-CD4bs antibody VRC01 (Science 329(5993):856-861), anti-V3 loop antibody 10-188 (PLoS One 6(9):e24078) and non-HIV-reactive antibody mGO53 (Science 301(5638):1374-1377) were used as controls. All experiments were performed in at least duplicates. Representative data are shown. [Figure 5-2] Figure 14. Binding of PGT121 mutants to gp120 "core" protein, gp120GD324-5AA mutant, and linear gp120V3 peptide. (B) Same as (A), but for binding to gp120GD324-5AA mutant protein. All experiments were performed at least twice. Representative data are shown. [Figure 5-3] Figure 1 shows binding of PGT121 mutants to gp120 "core" protein, gp120GD324-5AA mutant and linear gp120V3 peptide. (c) Bar graph comparing ELISA reactivity of PGT121-like and 10-1074-like antibodies and control antibodies (positive controls, 10-188, 1-79, 2-59 and 2-1261 (Nature 458(7238):636-640)) and negative control, mGO53) against gp120V3-C3 overlapping peptide. The y-axis shows ELISA values ​​(OD405nm) obtained by testing 2 μg / mL IgG antibodies. Amino acid sequences of individual peptides are shown at the bottom right. All experiments were performed at least twice. Representative data are shown. [Figure 6-1]Figure 1. Binding of PGT121 to gp120 glycosylation mutants and deglycosylated gp120. (A) ELISA-based binding analysis of PGT121 and 10-1074 antibody variants to gp120, gp120NNT301-303AAA, gp120N332A and gp120N332A / NNT301-303AAA. The x-axis indicates the antibody concentration (M) required to obtain the ELISA value (OD405nm) shown on the y-axis. The black dashed and continuous lines indicate the average reactivity against the four antigens of the positive (10-188) and negative (mGO53) antibody control groups. All experiments were performed at least twice. [Figure 6-2] Figure 1 shows binding of PGT121 to gp120 glycosylation mutants and deglycosylated gp120. (B) Silver stained SDS-PAGE gel comparing untreated gp120 (WT, wild type), PNGaseF digested gp120 and EndoH digested gp120. L, protein ladder. All experiments were performed at least twice. [Figure 6-3] Binding of PGT121 to gp120 glycosylation mutants and deglycosylated gp120. (C) Same as (A) but comparing untreated gp120 with PNGase F-treated gp120. All experiments were performed at least in duplicate. [Figure 6-4] Binding of PGT121 to gp120 glycosylation mutants and deglycosylated gp120. (D) Same as (A) but comparing untreated gp120 with EndoH-treated gp120. All experiments were performed at least in duplicate. [Figure 7-1]Figure 1 shows the binding of PGT121 and 10-1074 clonal variants to glycans. (A) Monosaccharide sequences of a set of 15 N-glycan probes used in glycan microarray analysis to investigate direct binding to N-glycans for PGT121-like and 10-1074-like antibodies. DH indicates the lipid tag 1,2-dihexadecyl-sn-glycero-3-phosphoethanolamine (DHPE) to which the N-glycans were attached by reductive amination. Important features to note are that (i) PGT121 group antibodies bound monoantennary N-glycan probe 10 (N2), which has a galactose-terminal antenna linked to the core mannose by a 1-3 linkage, but did not bind isomeric N-glycan probe 11 (designated N4), which has an antenna linked 1-6 to the core mannose; (ii) as in the case of biantennary probe 13 (NA2), the presence of this galactose-terminal 1-6 linked antenna permitted binding, as did the presence of α2-6 linked (rather than α2-3) sialic acid; and (iii) biantennary probe 12 (NGA2), which lacks galactose and terminates with N-acetylglucosamine, was not bound. [Figure 7-2] Figure 1 shows binding of PGT121 and 10-1074 clonal variants to glycans. (B) Bar graph comparing glycan binding by PGT121-like, 10-1074-like and germline (GL) antibodies. 10-188, an anti-V3 loop antibody, was used as a negative control. Numerical scores for binding were measured as fluorescence intensity (average of duplicate spots) for probes arrayed at 2 fmol (white) and 5 fmol (grey) per spot. [Figure 8-1]Figure 1. Antibody binding and neutralizing activity against high-mannose-only gp120 and virus. (A) Silver stained SDS-PAGE gel comparing YU-2 gp120 (gp120kif) produced in cells treated with kifunensine with gp120 (WT, wild type) produced in untreated cells. L, protein ladder. (B) ELISA comparison of binding of PGT121-like (blue label) and 10-1074-like (green label) antibodies to YU-2 gp120 (gp120WT) and gp120kif. The x-axis indicates the antibody concentration (M) required to obtain the ELISA value (OD405nm) shown on the y-axis. [Figure 8-2] Antibody binding and neutralizing activity against high-mannose-only gp120 and viruses. (C) Neutralization curves for PGT121 evaluated against selected PGT121-susceptible / 10-1074-resistant pseudoviruses produced in the presence (virus kif) or absence (virus WT) of kifunensine. The dashed horizontal line indicates 50% neutralization, and IC50 values ​​can be obtained from the antibody concentration on the x-axis. Experiments were performed in triplicate. Error bars indicate SD of triplicate determinations. (D) Bar graph comparing the neutralizing activity of selected antibodies against YU-2 and PVO.4 pseudoviruses produced in HEK293S GnTI- / - cells (virus GnT- / -) or in wild-type cells (virus WT). The y-axis indicates the mean IC50 values ​​(μg / mL) for neutralization of the viruses indicated on the x-axis. Error bars indicate SEM of IC50 values ​​obtained from two independent experiments. [Figure 9-1] Figure 1 shows the neutralizing activity of PGT121, 10-996 and 10-1074. (A) Graph comparing the neutralizing potency (determined using the TZM-bl assay and a panel of 119 pseudoviruses) of PGT121, 10-996 and 10-74 against viruses of the indicated HIV-1 clades. The x-axis shows the antibody concentration (μg / mL) required to achieve 50% neutralization (IC50). The y-axis shows the cumulative frequency of IC50 values ​​up to the concentration shown on the x-axis. [Figure 9-2](A) Neutralizing activity of PGT121, 10-996 and 10-1074. (B) Graph comparing neutralizing breadth and potency of PGT121, 10-996 and 10-1074 against an expanded panel of 119 viruses as determined by TZM-bl neutralization assay. The y-axis shows the cumulative frequency of IC80 values ​​up to the concentration shown on the x-axis. [Figure 9-3] Neutralizing activity of PGT121, 10-996 and 10-1074. (C) Graph shows neutralization curves of selected viruses by PGT121 and 10-1074. The dashed horizontal line indicates 50% neutralization and IC50 values ​​can be obtained from the antibody concentration on the x-axis. Experiments were performed in triplicate. Error bars indicate SD of triplicate determinations. [Figure 10] Figure 1. Neutralizing activity against clade B viruses, historical vs. current. Dot plots comparing neutralizing potency against clade B viruses isolated from historical (Hist.) and current (Cont.) seroconverters for select bNAbs. Horizontal bars represent median IC50 for all viruses per patient. Differences between groups were assessed using the Mann-Whitney test. ns, not significant. [Figure 11] Table showing neutralization of two R5-tropic SHIVs with a panel of 11 broadly active anti-HIV-1 mAbs. Calculated IC50 values ​​for neutralizing SHIVAD8EO (A) and SHIVDH12-V3AD8 (B). [Figure 12] FIG. 1 shows the relationship between plasma concentrations of passively administered neutralizing mAbs and viral acquisition following two different R5SHIV in macaques. Filled circles indicate protected (non-acquired) monkeys; open circles indicate infected animals. [Figure 13-1]1 is a table showing plasma concentrations of bNAbs. The concentrations of mAbs were determined by measuring neutralizing activity in plasma samples. (A) ID50 values ​​measured in the TZM.bl neutralization assay for 10-1074 and 3BNC117 against HIV-1 strains sensitive to one bNAb but not the other (i.e., HIV-1 strain X2088_9 (10-1074 sensitive); HIV-1 strain Q769_d22 (3BNC117 sensitive)). (B) Neutralizing activity of plasma before antibody administration (preP) but spiked with 0.01, 0.1, 1, 10 and 100 μg / mL of antibody 10-1074 (blue) or 3BNC117 (green). Neutralizing activity reported as plasma ID50 titers (left column) and converted to antibody concentrations (right column) based on the measured ID50 values ​​in (A). [Figure 13-2] 1 is a table showing plasma concentrations of bNAbs. The concentrations of mAbs were determined by measuring neutralizing activity in plasma samples. (C) ID50 titers (left column) and concentrations of bNAbs (right column) measured in the indicated macaque plasma samples before (pre-bleed) and after (days) bNAb administration. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0022] The present invention is based, at least in part, on the unexpected discovery of a novel category of broadly neutralizing antibodies (bNAbs) against HIV that can recognize carbohydrate-dependent epitopes, including complex-type N-glycans, on gp120.

[0023] Antibodies are essential for the success of most vaccines, and antibodies against HIV appear to be the only correlate of protection in the recent RV144 anti-HIV vaccine trial. Some HIV-1-infected patients develop broadly neutralizing serum activity against the gp160 viral spike 2-4 years after infection, but these antibodies do not generally protect infected humans because of autologous viral escape by mutation. Nevertheless, broadly neutralizing activity exerts selective pressure against the virus, and passive transfer of broadly neutralizing antibodies (bNAbs) to macaques is protective against SHIV infection. It has therefore been proposed that a vaccine eliciting such antibodies could be protective against HIV infection in humans.

[0024] The development of single-cell antibody cloning methods has revealed that bNAbs target several different epitopes on the HIV-1 gp160 spike. Most potent HIV-1 bNAbs recognize the CD4 binding site (CD4bs) (Science 333(6049):1633-1637; Nature 477(7365):466-470; Science 334(6060):1289-1293) and carbohydrate-dependent epitopes involving variable loops (Nature 477(7365):466-470; Science 326(5950):285-289; Science 334(6059):1097-1103; Nature 477(7365):466-470; Science 326(5950):285-289; Science 334(6059):1097-1103; Nature 477(7365):466-470; Science 333(6049):1633-1637; Nature 477(7365):466-470; Science 334(6060):1289-1293). 480(7377):336-343). Because the antibodies studied to date are unique examples or members of small clonal families, little is known about carbohydrate-dependent epitopes.

[0025] To better understand neutralizing antibody responses against HIV-1 and epitopes targeted by PGT antibodies, we isolated members of a large clonal family that dominates gp160-specific IgG memory responses from a clade A-infected patient who produced PGT121. As disclosed herein, PGT121 antibodies are divided into two groups, the PGT121-like group and the 10-1074-like group, according to sequence, binding affinity, neutralizing activity, and carbohydrate and V3 loop recognition. 10-1074 and related family members exhibit unusually potent neutralization, including broad reactivity against novel transmissible viruses. Unlike previously characterized carbohydrate-dependent bNAbs, PGT121 binds complex N-glycans rather than high mannose in glycan microassay experiments. The crystal structures of PGT121 and 10-1074, compared to the structures of their germline precursors and the structure of PGT121 bound to complex N-glycans, rationalize their unique properties.

[0026] In one example, assays were performed to isolate B cell clones encoding PGT121, which is unique among glycan-dependent bNabs in that it recognizes complex N-glycans but not high mannose. PGT121 clones fall into PGT121-like and 10-1074-like groups, which are differentiated by sequence, binding affinity, carbohydrate recognition, and neutralizing activity. The 10-1074 group exhibits remarkable potency and breadth despite not detectably binding protein-free glycans. Crystal structures of unliganded PGT121, 10-1074, and their germline precursors revealed that carbohydrate recognition differences map to a cleft between CDRH2 and CDRH3, which was occupied by complex N-glycans in the isolated PGT121 structure. Exchange of glycan contact residues between PGT121 and 10-1074 confirmed the importance of these residues in neutralizing activity. The HIV envelope displays a variety of high mannose and complex type N-glycans, and therefore these results, including the first structural characterization of complex type N-glycan recognition by anti-HIV bNAbs, are important for understanding how antibodies and ultimately vaccines can achieve broadly neutralizing activity.

[0027] The term "antibody" (Ab) as used herein includes monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g., bispecific and polyreactive antibodies), and antibody fragments. Thus, the term "antibody" as used in any context herein is intended to include, but is not limited to, any specific binding member, immunoglobulin class and / or isotype (e.g., IgG1, IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, and IgM); and biologically relevant fragments or specific binding members thereof (including, but not limited to, Fab, F(ab')2, Fv, and scFv (single chain or related entities)). Antibodies are understood in the art to be glycoproteins having at least two heavy (H) chains and two light (L) chains interconnected by disulfide bonds, or antigen-binding portions thereof. The heavy chain is composed of a heavy chain variable region (VH) and a heavy chain constant region (CH1, CH2, and CH3). The light chain is composed of a light chain variable region (VL) and a light chain constant region (CL). Both the heavy and light chain variable regions contain framework regions (FWR) and complementarity determining regions (CDR). The four FWR regions are relatively conserved, while the CDR regions (CDR1, CDR2 and CDR3) correspond to hypervariable regions and are arranged from the NH2-terminus to the COOH-terminus as follows: FWR1, CDR1, FWR2, CDR2, FWR3, CDR3 and FWR4. The variable regions of the heavy and light chains contain binding domains that interact with antigens, while the constant region(s) can mediate the binding of the immunoglobulin to host tissues or factors, depending on the isotype.

[0028] The definition of "antibody" as used herein also includes chimeric, humanized and recombinant antibodies, human antibodies produced from transgenic non-human animals, and antibodies selected from libraries using enrichment techniques available to those of skill in the art.

[0029] The term "variable" refers to the fact that certain segments of the variable (V) domains differ significantly in sequence among antibodies. The V domains mediate antigen binding and define the specificity of a particular antibody for its particular antigen. However, the variability is not uniformly distributed throughout the 110 amino acid span of the variable regions. Instead, the V regions consist of relatively invariant stretches of 15-30 amino acids called framework regions (FRs) separated by shorter regions of hypervariability called "hypervariable regions", each of which is 9-12 amino acids long. Naturally occurring heavy and light chain variable regions each contain four FRs, primarily adopting a β-sheet structure, connected by three hypervariable regions that form loops that connect, and in some cases form part of, the β-sheet structure. The hypervariable regions in each chain are held in close proximity to each other by the FRs and, together with the hypervariable regions from the other chain, contribute to the formation of the antigen-binding site of antibodies (see, e.g., Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991)).

[0030] The term "hypervariable region" as used herein refers to the amino acid residues of an antibody which are involved in antigen binding. The hypervariable region generally comprises amino acid residues from the "complementarity determining regions" (CDRs).

[0031] The term "monoclonal antibody" as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present in minor amounts. The term "polyclonal antibody" refers to a preparation containing different antibodies directed against different determinants ("epitopes").

[0032] Monoclonal antibodies herein include "chimeric" antibodies in which a portion of the heavy and / or light chain is identical or homologous to a corresponding sequence in an antibody from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain(s) is identical or homologous to a corresponding sequence in an antibody from another species or belonging to another antibody class or subclass, as well as fragments of such antibodies, so long as the fragments exhibit the desired biological activity (see, e.g., U.S. Pat. No. 4,816,567; and Morrison et al., Proc. Natl. Acad. Sci. USA, 81:6851-6855 (1984)). The described invention provides variable region antigen-binding sequences derived from human antibodies. Thus, chimeric antibodies of primary interest herein include antibodies having one or more human antigen-binding sequences (e.g., CDRs) and containing one or more sequences, e.g., FR or C-region sequences, derived from a non-human antibody. In addition, chimeric antibodies included herein are those that contain human variable region antigen-binding sequences of one antibody class or subclass and other sequences, such as FR or C region sequences, derived from another antibody class or subclass.

[0033] A "humanized antibody" is generally considered to be a human antibody having one or more amino acid residues introduced from a source that is non-human. These non-human amino acid residues are often referred to as "import" residues, which typically come from an "import" variable region. Humanization may be performed according to the method of Winter and coworkers by substituting the imported hypervariable sequences for the corresponding sequences of a human antibody (see, e.g., Jones et al., Nature, 321:522-525 (1986); Reichmann et al., Nature, 332:323-327 (1988); Verhoeyen et al., Science, 239:1534-1536 (1988)). Such "humanized" antibodies are thus chimeric antibodies (see, e.g., U.S. Pat. No. 4,816,567) in which substantially less of the variable regions of an intact human variable region have been substituted by the corresponding sequences from a non-human species.

[0034] An "antibody fragment" includes a portion of an intact antibody, such as the antigen-binding or variable region of the intact antibody. Examples of antibody fragments include, but are not limited to, Fab, Fab', F(ab')2 and Fv fragments; diabodies; linear antibodies (see, e.g., U.S. Pat. No. 5,641,870; Zapata et al., Protein Eng. 8(10):1057-1062

[1995] ); single-chain antibody molecules; and multispecific antibodies formed from antibody fragments.

[0035] "Fv" is the minimum antibody fragment that contains a complete antigen recognition and binding site. This fragment contains a dimer of one heavy and one light chain variable domain in tight, non-covalent association. Folding of these two domains results in six hypervariable loops (three loops each from the H and L chains), which contribute amino acid residues for antigen binding and confer antigen-binding specificity to the antibody. However, even a single variable region (or half of an Fv, containing only three antigen-specific CDRs) has the ability to recognize and bind antigen, albeit with a lower affinity than the entire binding site.

[0036] A "single-chain Fv" ("sFv" or "scFv") is an antibody fragment comprising the VH and VL antibody domains connected into a single polypeptide chain. The sFv polypeptide can further comprise a polypeptide linker between the VH and VL domains which enables the sFv to form the desired structure for antigen binding. For a review of sFvs, see, e.g., Pluckthun in The Pharmacology of Monoclonal Antibodies, vol. 113, Rosenburg and Moore eds., Springer-Verlag, New York, pp. 269-315 (1994); Borrebaeck 1995, infra.

[0037] The term "diabody" refers to small antibody fragments prepared by constructing sFv fragments with a short linker (about 5-10 residues) between the VH and VL domains such that interchain rather than intrachain pairing of the V domains is achieved, resulting in a bivalent fragment, i.e., a fragment with two antigen binding sites. Bispecific diabodies are heterodimers of two "crossover" sFv fragments in which the VH and VL domains of the two antibodies are present on different polypeptide chains. Diabodies are more fully described, for example, in EP 404,097; WO 93 / 11161; and Hollinger et al., Proc. Natl. Acad. Sci. USA, 90:6444-6448 (1993).

[0038] Domain antibodies (dAbs) that can be produced in fully human form are the smallest known antigen-binding fragments of antibodies, ranging from about 11 kDa to about 15 kDa. dAbs are the robust variable regions of the heavy and light chains of immunoglobulins (VH and VL, respectively). They are highly expressed in microbial cell cultures, exhibit favorable biophysical properties, including but not limited to, solubility and temperature stability, and are well suited for selection and affinity maturation by in vitro selection systems, such as phage display. dAbs are biologically active as monomers, and due to their small size and inherent stability, can be formatted into larger molecules to create drugs with extended serum half-lives or other pharmacological activity. Examples of these techniques are described, for example, in WO 9425591 for antibodies derived from camelid heavy chain Ig, and in addition, US 20030130496 describes the isolation of single domain fully human antibodies from phage libraries.

[0039] Fv and sFv are the only species with intact binding sites that are completely devoid of constant regions. Therefore, they are suitable for reducing non-specific binding during in vivo use. sFv fusion proteins can be constructed to produce fusion of effector proteins at either the amino or carboxy terminus of sFv. See, for example, Antibody Engineering, ed. Borrebaeck, supra. The antibody fragment can also be, for example, a "linear antibody," as described, for example, in U.S. Pat. No. 5,641,870. Such linear antibody fragments can be monospecific or bispecific.

[0040] In some embodiments, the antibodies of the described invention are bispecific or multispecific. Bispecific antibodies are antibodies that have binding specificities for at least two different epitopes. As an example, bispecific antibodies can bind to two different epitopes of a single antigen. Other such antibodies can combine a first antigen binding site with a binding site for a second antigen. Alternatively, anti-HIV arms can be combined with arms that bind to trigger molecules on white blood cells, such as T cell receptor molecules (e.g., CD3), or Fc receptors for IgG (Fc gamma R), such as Fc gamma RI (CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16), to focus and localize cellular defense mechanisms to infected cells. Bispecific antibodies can also be used to localize cytocidal agents to infected cells. Bispecific antibodies can be prepared as full-length antibodies or antibody fragments (e.g., F(ab')2 bispecific antibodies). For example, WO 96 / 16673 describes a bispecific anti-ErbB2 / anti-Fc gamma RIII antibody, and U.S. Patent No. 5,837,234 discloses a bispecific anti-ErbB2 / anti-Fc gamma RI antibody. For example, a bispecific anti-ErbB2 / anti-Fc alpha antibody is reported in WO 98 / 02463; U.S. Patent No. 5,821,337 teaches a bispecific anti-ErbB2 / anti-CD3 antibody. See, e.g., Mouquet et al., Polyreactivity Increases The Apparent Affinity Of Anti-HIV Antibodies By Heteroligation. Nature. 467, 591-5 (2010); and also Mouquet et al., Enhanced HIV-1 neutralization by antibody heteroligation" Proc Natl Acad Sci US A. 2012 Jan 17; 109(3):875-80.

[0041] Methods for producing bispecific antibodies are known in the art. Classical production of full-length bispecific antibodies is based on the co-expression of two immunoglobulin heavy-light chain pairs, where the two chains have different specificities (see, for example, Millstein et al., Nature, 305:537-539 (1983)). Similar procedures are disclosed, for example, in WO 93 / 08829, Traunecker et al., EMBO J., 10:3655-3659 (1991). See also Mouquet et al., Enhanced HIV-1 neutralization by antibody heteroligation" Proc Natl Acad Sci US A. 2012 Jan 17; 109(3):875-80.

[0042] Alternatively, antibody variable regions with the desired binding specificity (antibody-antigen binding site) are fused to immunoglobulin constant domain sequences. The fusion is with an Ig heavy chain constant region, including at least a portion of the hinge, CH2 and CH3 regions. According to some embodiments, the first heavy chain constant region (CH1), containing the site necessary for light chain binding, is present in at least one of the fusions. DNA encoding the immunoglobulin heavy chain fusions and, if desired, the immunoglobulin light chain, are inserted into separate expression vectors and co-transfected into a suitable host cell. This allows more freedom in adjusting the mutual ratio of the three polypeptide fragments in embodiments where unequal ratios of the three polypeptide chains used in the construction give the optimal yield of the desired bispecific antibody. However, it is possible to insert the coding sequences for two or all three polypeptide chains into a single expression vector when expression of equal ratios of at least two polypeptide chains results in high yields or when said ratios do not significantly affect the yield of the desired chain combination.

[0043] Techniques for generating bispecific antibodies from antibody fragments are also described in the literature. For example, bispecific antibodies can be prepared using chemical linkage. For example, Brennan et al., Science, 229:81 (1985) describes a procedure in which intact antibodies are proteolytically cleaved to generate F(ab')2 fragments. These fragments are reduced in the presence of a dithiol complexing agent, sodium arsenite, to stabilize vicinal dithiols and prevent intermolecular disulfide formation. The generated Fab' fragments are then converted to thionitrobenzoate (TNB) derivatives. One of the Fab'-TNB derivatives is then reconverted to a Fab'-thiol by reduction with mercaptoethylamine and mixed with an equimolar amount of the other Fab'-TNB derivative to form the bispecific antibody. The generated bispecific antibody can be used as an agent for the selective immobilization of enzymes.

[0044] Other modifications of the antibody are contemplated herein. For example, the antibody can be attached to one of a variety of non-proteinaceous polymers, such as polyethylene glycol, polypropylene glycol, polyoxyalkylene, or copolymers of polyethylene glycol and polypropylene glycol. The antibody can also be entrapped in microcapsules (e.g., hydroxymethylcellulose, or gelatin-microcapsules and poly(methyl methacrylate) microcapsules, respectively), prepared, for example, by coacervation or by interfacial polymerization, in colloidal drug delivery systems (e.g., liposomes, albumin microspheres, microemulsions, nanoparticles, and nanocapsules), or in macroemulsions. Such techniques are disclosed, for example, in Remington's Pharmaceutical Sciences, 16th edition, Oslo, A., Ed., (1980).

[0045] Typically, the antibodies of the described invention are recombinantly produced using vectors and methods available in the art. Human antibodies can also be produced by in vitro activated B cells (see, e.g., U.S. Patent Nos. 5,567,610 and 5,229,275). General methods of molecular genetics and genetic engineering useful in the present invention are described in Molecular Cloning: A Laboratory Manual (Sambrook, et al., 1989, Cold Spring Harbor Laboratory Press), Gene Expression Technology (Methods in Enzymology, Vol. 185, edited by D. Goeddel, 1991. Academic Press, San Diego, CA), "Guide to Protein Purification" in Methods in Enzymology (MP Deutscher, ed., (1990) Academic Press, Inc.); PCR Protocols: A Guide to Methods and Applications (Innis, et al. 1990. Academic Press, San Diego, CA), Culture of Animal Cells: A Manual of Basic Technique, 2nd Ed. (RI Freshney. 1987. Liss, Inc. New York, NY), and Gene Transfer and Expression Protocols, pp. 109-128, ed. EJ Murray, The Humana Press (Current editions of the American Society of Molecular Biology, Inc., Clifton, NJ). Reagents, cloning vectors and kits for genetic manipulation are available from commercial vendors, e.g., BioRad, Stratagene, Invitrogen, ClonTech and Sigma-Aldrich Co.

[0046] Human antibodies can also be produced in transgenic animals (e.g., mice) that are capable of producing the full repertoire of human antibodies in the absence of endogenous immunoglobulin production. For example, homozygous deletion of the antibody heavy chain joining region (JH) gene in chimeric and germline mutant mice has been described to result in complete inhibition of endogenous antibody production. Transfer of the human germline immunoglobulin gene array into such germline mutant mice results in the production of human antibodies upon antigen challenge. See, e.g., Jakobovits et al., Proc. Natl. Acad. Sci. USA, 90:2551 (1993); Jakobovits et al., Nature, 362:255-258 (1993); Bruggemann et al., Year in Immuno., 7:33 (1993); U.S. Patent Nos. 5,545,806, 5,569,825, and 5,591,669 (all GenPharm); U.S. Patent No. 5,545,807; and WO 97 / 17852. Such animals can be genetically engineered to produce human antibodies comprising the polypeptides of the described invention.

[0047] Various techniques have been developed for the production of antibody fragments. Traditionally, these fragments were obtained by proteolytic digestion of intact antibodies (see, e.g., Morimoto et al., Journal of Biochemical and Biophysical Methods 24:107-117 (1992); and Brennan et al., Science, 229:81 (1985)). However, these fragments can now be produced directly by recombinant host cells. Fab, Fv and ScFv antibody fragments can all be expressed in and secreted from E. coli, allowing the facile production of large amounts of these fragments. Fab'-SH fragments can be directly recovered from E. coli and chemically coupled to form F(ab')2 fragments (see, e.g., Carter et al., Bio / Technology 10:163-167 (1992)). According to another approach, F(ab')2 fragments can be directly isolated from recombinant host cell culture. Fab and F(ab')2 fragments with increased in vivo half-lives that contain salvage receptor binding epitope residues are described in U.S. Patent No. 5,869,046. Other methods for producing antibody fragments will be apparent to those skilled in the art.

[0048] Other techniques known in the art for selecting antibody fragments from libraries using enrichment methods, including, but not limited to, phage display, ribosome display (Hanes and Pluckthun, 1997, Proc. Nat. Acad. Sci. 94:4937-4942), bacterial display (Georgiou, et al., 1997, Nature Biotechnology 15:29-34) and / or yeast display (Kieke, et al., 1997, Protein Engineering 10: 1303-1310), may be used as alternatives to the techniques discussed above to select single chain antibodies. Single chain antibodies are selected from libraries of single chain antibodies produced directly using filamentous phage technology. Phage display technology is known in the art (e.g., U.S. Pat. Nos. 5,565,332, 5,733,743, 5,871,907, 5,872,215, 5,885,793, 5,962,255, 6,140,471, 6,225,447, 6,291650, 6,49 See US Patent Nos. 2,160, 6,521,404, 6,544,731, 6,555,313, 6,582,915, 6,593,081, and other members of the US patent family, or technology from Cambridge Antibody Technology (CAT) as disclosed in the priority application based on British Patent No. 9206318 filed May 24, 1992; see also Vaughn, et al. 1996, Nature Biotechnology 14:309-314). Single chain antibodies may be designed and constructed using available recombinant DNA technology, such as DNA amplification methods (e.g., PCR), or possibly by using the respective hybridoma cDNA as a template.

[0049] Mutant antibodies are also included within the scope of the present invention. Thus, variants of the sequences described in this application are also included within the scope of the present invention. Further variants of antibody sequences with improved affinity can be obtained using methods known in the art, and these variants are within the scope of the present invention. For example, amino acid substitutions can be used to obtain antibodies with further improved affinity. Alternatively, codon optimization of nucleotide sequences can be used to improve the translation efficiency in expression systems for antibody production.

[0050] Such variant antibody sequences will share 70% or more (i.e., 80%, 85%, 90%, 95%, 97%, 98%, 99% or more) sequence identity with the sequences described in this application. Such sequence identity is calculated relative to the full length of the reference sequence (i.e., the sequence described in this application). As referred to herein, percent identity is as determined using BLAST version 2.1.3 with the default parameters [Blosum 62 matrix; gap opening penalty=11 and gap extension penalty=1] specified by NCBI (National Center for Biotechnology Information; www.ncbi.nlm.nih.gov / ). For example, peptide sequences comprising at least about 5, 10, 15, 20, 30, 40, 50, 75, 100, 150 or more contiguous peptides of one or more of the sequences disclosed herein, and all intermediate length peptides therebetween, are provided by the present invention. As used herein, the term "intermediate length" is intended to describe any length between the cited values, e.g., 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, etc.; 21, 22, 23, etc.; 30, 31, 32, etc.; 50, 51, 52, 53, etc.; 100, 101, 102, 103, etc., 150, 151, 152, 153, etc.

[0051] The present invention provides antibodies with broad neutralizing activity in serum, alone or in combination with other antibodies, such as, but not limited to, VRC01, anti-V3 loop, CD4bs and CD4i antibodies, and PG9 / PG16-like antibodies.

[0052] According to another embodiment, the invention provides methods for preparing and administering HIV antibody compositions suitable for administration to a human or non-human primate patient infected with or at risk of HIV infection in an amount and on a schedule sufficient to induce a protective immune response against HIV or reduction of HIV viral load in humans.

[0053] According to another embodiment, the present invention provides a vaccine comprising at least one antibody of the present invention and a pharma- ceutically acceptable carrier. According to one embodiment, the vaccine is a vaccine comprising at least one antibody as described herein and a pharma- ceutically acceptable carrier. The vaccine may comprise any combination of antibodies having the properties described herein, and may further comprise an antibody that neutralizes HIV, as known in the art.

[0054] It is understood that the composition may be a single antibody disclosed herein or a combination of the same or different antibodies disclosed herein to prophylactically or therapeutically treat the progression of HIV infection of various subtypes after vaccination. Such combinations can be selected according to the desired immunity. When the antibody is administered to an animal or human, it can be combined with one or more pharma- ceutically acceptable carriers, excipients or adjuvants as known to those of ordinary skill in the art. The composition can further include broadly neutralizing antibodies known in the art, including but not limited to VRC01, b12, anti-V3 loop, CD4bs and CD4i antibodies, and PG9 / PG16-like antibodies.

[0055] Furthermore, particularly with regard to determining the efficacy level in patients for the treatment of HIV, suitable animal models are available and have been widely implemented to evaluate the in vivo efficacy of various gene therapy protocols against HIV (Sarver et al. (1993b), supra). These models include mice, monkeys and cats. Even though these animals are not naturally susceptible to HIV disease, chimeric mouse models (e.g., SCID, bg / nu / xid, NOD / SCID, SCID-hu, immunocompetent SCID-hu, myeloablated BALB / c) reconstituted with human peripheral blood mononuclear cells (PBMC), lymph nodes, fetal liver / thymus or other tissues can be infected with lentiviral vectors or HIV and used as models of HIV pathogenesis. Similarly, a simian immunodeficiency virus (SIV) / monkey model can be used, as can a feline immunodeficiency virus (FIV) / cat model. The pharmaceutical composition, when used for the therapeutic treatment of AIDS, can contain other medicaments together with the vector according to the present invention. These other medicaments can be used in their traditional manner (i.e., as agents for treating HIV infection).

[0056] According to another embodiment, the present invention provides an antibody-based pharmaceutical composition comprising an effective amount of an isolated HIV antibody, or an affinity matured version, which provides a prophylactic or therapeutic treatment option for reducing the infection of the HIV virus. The antibody-based pharmaceutical composition of the present invention can be formulated by any number of strategies known in the art (see, for example, McGoff and Scher, 2000, Solution Formulation of Proteins / Peptides: In McNally, EJ, ed. Protein Formulation and Delivery. New York, NY: Marcel Dekker; pp. 139-158; Akers and Defilippis, 2000, Peptides and Proteins as Parenteral Solutions. In: Pharmaceutical Formulation Development of Peptides and Proteins. Philadelphia, PA: Talyor and Francis; pp. 145-177; Akers, et al., 2002, Pharm. Biotechnol. 14: 47-127). A pharma- ceutically acceptable composition suitable for administration to a patient will contain an effective amount of the antibody in a formulation that retains biological activity during storage within an acceptable temperature range while also promoting maximum stability. The pharmaceutical composition may also include a pharma- ceutically acceptable diluent, a pharma-ceutically acceptable carrier and / or a pharma-ceutically acceptable excipient, or such vehicle that is commonly used in formulating pharmaceutical compositions for administration to animals or humans, depending on the formulation desired. The diluent is selected so as not to affect the biological activity of the combination. Examples of such diluents are distilled water, phosphate buffered saline, Ringer's solution, dextrose solution, and Hank's solution. The amount of excipient useful in the pharmaceutical composition or formulation of the present invention is an amount that serves to distribute the antibody evenly throughout the composition so that the composition can be evenly dispersed when it is to be administered to a subject in need thereof.It may be useful to dilute the antibody to a concentration that provides the desired beneficial palliative or curative result while minimizing any adverse side effects that may result from too high a concentration. It may also have a preservative effect. Thus, for antibodies with high biological activity, more excipients will be utilized. On the other hand, for any active ingredient(s) that exhibit lower biological activity, lesser amounts of excipients will be utilized.

[0057] The above antibodies, as well as antibody or vaccine compositions comprising at least one or a combination of the antibodies described herein, can be administered for the prophylactic and therapeutic treatment of HIV viral infection.

[0058] The present invention also relates to isolated polypeptides comprising the novel amino acid sequences of the light and heavy chains, as well as the consensus sequences for the heavy and light chains of SEQ ID NOs: 1 and 2, as listed in FIG.

[0059] In other related embodiments, the invention provides polypeptide variants that encode the amino acid sequences of the HIV antibodies listed in Figure 3; the consensus sequences for the heavy and light chains of SEQ ID NOs: 1 and 2. These polypeptide variants have at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% or more sequence identity compared to the polypeptide sequences of the invention as determined using the methods described herein (e.g., BLAST analysis using standard parameters). It will be understood by those of skill in the art that these values ​​can be appropriately adjusted to determine the corresponding identity of the encoded proteins, taking into account amino acid similarity and the like.

[0060] The term "polypeptide" is used in its conventional sense, i.e., as a sequence of amino acids. A polypeptide is not limited to a particular length of the product. Peptides, oligopeptides, and proteins are included in the definition of a polypeptide, and such terms may be used interchangeably herein, unless otherwise indicated. The term also includes post-expression modifications of the polypeptide, both naturally occurring and non-naturally occurring, such as glycosylation, acetylation, phosphorylation, and other modifications known in the art. A polypeptide may be an entire protein or a subsequence thereof. A particular polypeptide of interest in the context of the present invention is an amino acid subsequence that includes a CDR, a VH, and a VL, capable of binding to an antigen or an HIV-infected cell.

[0061] A polypeptide "variant," as that term is used herein, is a polypeptide that typically differs from a polypeptide specifically disclosed herein by one or more substitutions, deletions, additions, and / or insertions. Such variants may be of natural origin or may be synthetically produced, for example, by modifying one or more of the above polypeptide sequences of the invention and evaluating one or more biological activities of the polypeptides described herein, and / or by using any of a number of techniques well known in the art.

[0062] For example, certain amino acids can be substituted for other amino acids in the protein structure without significant loss of ability to bind to other polypeptides (e.g., antigens) or cells. Because the binding ability and properties of a protein define its biological functional activity, certain amino acid sequence substitutions can be made in the protein sequence, and thus in the underlying DNA coding sequence, to obtain a protein with similar properties. Thus, it is contemplated that various changes can be made to the peptide sequences of the disclosed compositions, or the corresponding DNA sequences encoding said peptides, without significant loss of their biological usefulness or activity.

[0063] In many cases, a polypeptide variant will contain one or more conservative substitutions, which are substitutions of one amino acid with another having similar properties that would be expected by one skilled in the art of peptide chemistry to leave the secondary structure and hydropathic properties of the polypeptide substantially unchanged.

[0064] Amino acid substitutions are generally based on the relative similarity of the amino acid side-chain substituents, e.g., their hydrophobicity, hydrophilicity, charge, size, etc. For example, substitutions that take into account the various aforementioned properties are well known to those of skill in the art and include arginine for lysine, glutamate for aspartate, serine for threonine, glutamine for asparagine, and valine for leucine and isoleucine.

[0065] "Homology" or "sequence identity" refers to the percentage of residues in a polynucleotide or polypeptide sequence variation that are identical to the non-variant sequence after aligning the sequences and, if necessary, inserting gaps to achieve maximum homology. In certain embodiments, polynucleotide and polypeptide variants have at least about 70%, at least about 75%, at least about 80%, at least about 90%, at least about 95%, at least about 98%, or at least about 99% polynucleotide or polypeptide homology with the polynucleotides or polypeptides described herein.

[0066] Such variant polypeptide sequences will share 70% or more (i.e., 80%, 85%, 90%, 95%, 97%, 98%, 99% or more) sequence identity with the sequences described in the present application. In further embodiments, the described invention provides polypeptide fragments comprising contiguous stretches of various lengths of the amino acid sequences disclosed herein. For example, peptide sequences comprising at least about 5, 10, 15, 20, 30, 40, 50, 75, 100, 150 or more contiguous peptides of one or more of the sequences disclosed herein, and all intermediate lengths therebetween, are provided by the present invention.

[0067] The present invention also includes nucleic acid sequences encoding part or all of the light and heavy chains of the antibodies of the present invention, and fragments thereof. Due to redundancy in the genetic code, there will be variants of these sequences that encode the same amino acid sequences.

[0068] The present invention also includes isolated nucleic acid sequences encoding peptides of the heavy and light chains of the HIV antibodies listed in FIG. 3, as well as the heavy and light chain consensus sequences of SEQ ID NOs:1 and 2.

[0069] In other related embodiments, the described invention provides polynucleotide variants that encode the polypeptide sequences of the heavy and light chains of the HIV antibodies listed in Figure 3; the consensus sequences of the heavy and light chains of SEQ ID NOs: 1 and 2. These polynucleotide variants have at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% or more sequence identity compared to the polynucleotide sequences of the invention as determined using the methods described herein (e.g., BLAST analysis using standard parameters). It will be understood by those skilled in the art that these values ​​can be appropriately adjusted to determine the corresponding identity of the proteins encoded by the two nucleotide sequences by taking into account codon degeneracy, amino acid similarity, reading frame alignment, and the like.

[0070] The terms "nucleic acid" and "polynucleotide" are used interchangeably herein to refer to single- or double-stranded RNA, DNA, or mixed polymers. Polynucleotides can include genomic sequences, additional genomic and plasmid sequences, and smaller engineered gene segments that express or can be adapted to express a polypeptide.

[0071] An "isolated nucleic acid" is a nucleic acid that is substantially separated from other genomic DNA as well as from proteins or complexes that naturally accompany the native sequence, such as ribosomes and polymerases. The term encompasses a nucleic acid sequence that has been removed from its naturally occurring environment, including recombinant or cloned DNA isolates, and chemically synthesized analogs, or analogs biosynthesized by heterologous systems. A substantially pure nucleic acid includes said nucleic acid in isolated form. Thus, it refers to a nucleic acid as originally isolated, and does not exclude genes or sequences that are later added to the isolated nucleic acid by the hand of man.

[0072] A polynucleotide "variant," as the term is used herein, refers to a polynucleotide that typically differs from the polynucleotides specifically disclosed herein by one or more substitutions, deletions, additions, and / or insertions. Such variants may be of natural origin or may be synthetically generated, for example, by one or more modifications of a polynucleotide sequence of the invention and evaluating one or more biological activities of the encoded polypeptides described herein, and / or by using any of a number of techniques well known in the art.

[0073] Modifications can be made to the structure of the polynucleotides of the present invention to obtain functional molecules that encode mutant or derivative polypeptides with desired properties. When it is desired to modify the amino acid sequence of a polypeptide to produce an equivalent or even improved mutant or portion of a polypeptide of the present invention, typically one skilled in the art will change one or more of the codons of the encoding DNA sequence.

[0074] Typically, the polynucleotide variants contain one or more substitutions, additions, deletions and / or insertions such that the immunogenic binding properties of the polypeptides encoded by the variant polynucleotides are not substantially diminished compared to the polypeptides encoded by the polynucleotide sequences specifically set forth herein.

[0075] In further embodiments, the described invention provides polynucleotide fragments comprising contiguous stretches of various lengths of sequences identical or complementary to one or more of the sequences disclosed herein. For example, polynucleotides comprising at least about 10, 15, 20, 30, 40, 50, 75, 100, 150, 200, 300, 400, 500, or 1000 or more contiguous nucleotides of one or more of the sequences disclosed herein and all intermediate lengths therebetween, including any length between the cited values, for example, 16, 17, 18, 19, etc., 21, 22, 23, etc., 30, 31, 32, etc., 50, 51, 52, 53, etc., 100, 101, 102, 103, etc., 150, 151, 152, 153, etc., and all integers between 200 and 500, 500 and 1000, are provided by the present invention.

[0076] In another embodiment of the present invention, a polynucleotide composition is provided that can hybridize to the polynucleotide sequences provided herein, or fragments thereof, or complementary sequences thereof, under medium to high stringency conditions. Hybridization methods are well known in the molecular biology arts. For illustrative purposes, suitable moderately stringent conditions for testing hybridization of the polynucleotides of the present invention with other polynucleotides include a prewash in a solution of 5xSSC, 0.5% SDS, 1.0 mM EDTA (pH 8.0); overnight hybridization at 50-60°C in 5xSSC; followed by two washes at 65°C for 20 minutes each in 2x, 0.5x and 0.2xSSC containing 0.1% SDS. Those skilled in the art will appreciate that the stringency of hybridization can be easily manipulated, for example, by varying the salt content of the hybridization solution and / or the temperature at which hybridization is performed. For example, in another embodiment, suitable highly stringent hybridization conditions include those described above, except that the temperature of hybridization is increased to, for example, 60-65°C or 65-70°C.

[0077] In some embodiments, the polypeptides encoded by the polynucleotide variants or fragments have the same binding specificity (i.e., specifically or preferentially bind to the same epitope or HIV strain) as the polypeptides encoded by the native polynucleotides. In some embodiments, the presently described polynucleotides, polynucleotide variants, fragments and hybridization sequences encode polypeptides having a level of binding activity at least about 50%, at least about 70%, and at least about 90% of that for the polypeptide sequences specifically set forth herein.

[0078] The polynucleotides of the described invention, or fragments thereof, regardless of the length of the coding sequence itself, can be combined with other DNA sequences, such as promoters, polyadenylation signals, additional restriction enzyme sites, multiple cloning sites, other coding segments, etc., and therefore their total length can vary considerably. Nucleic acid fragments of almost any length can be used. For example, exemplary polynucleotide segments having total lengths of about 10,000, about 5,000, about 3,000, about 2,000, about 1,000, about 500, about 200, about 100, about 50 base pairs, etc. (including all intermediate lengths) are included in many implementations of the present invention.

[0079] Vectors, such as expression vectors, comprising a nucleic acid sequence according to the invention are further included within the scope of the invention. Cells transformed with such vectors are also included within the scope of the invention.

[0080] The present invention also provides vectors and host cells comprising the nucleic acids of the present invention, as well as recombinant methods for producing the polypeptides of the present invention. Vectors of the present invention include those that can replicate in any type of cell or organism, including, for example, plasmids, phages, cosmids, and minichromosomes. In some embodiments, the vectors comprising the polynucleotides of the present invention are suitable for the growth or replication of the polynucleotides, or are suitable for the expression of the polypeptides of the present invention. Such vectors are known in the art and commercially available.

[0081] "Vector" includes shuttle vectors and expression vectors. Typically, a plasmid construct will also include an origin of replication (e.g., ColE1 origin of replication) and a selection marker (e.g., ampicillin or tetracycline resistance) for replication and selection, respectively, of bacterial plasmids. "Expression vector" refers to a vector that contains control sequences or regulatory elements necessary for expression of antibodies, including antibody fragments, of the invention in bacterial or eukaryotic cells.

[0082] As used herein, the term "cell" can be any cell, including, but not limited to, of a eukaryotic, multicellular species, such as, but not limited to, a mammalian cell or a human cell (as opposed to, e.g., a unicellular yeast cell). A cell may exist as a single entity or may be part of a larger collection of cells. Such a "larger collection of cells" can include, for example, a cell culture (mixed or pure), a tissue (e.g., endothelial, epithelial, mucosal or other tissue), an organ (e.g., lung, liver, muscle and other organs), an organ system (e.g., circulatory system, respiratory system, gastrointestinal system, urinary system, nervous system, integumentary system or other organ system), or an organism (e.g., bird, mammal, etc.).

[0083] The polynucleotides of the invention may be synthesized in whole or in portions and assembled and inserted into a vector using routine molecular and cell biology techniques, including, for example, subcloning the polynucleotide into a linear vector using appropriate restriction sites and restriction enzymes. The polynucleotides of the described invention are amplified by the polymerase chain reaction using oligonucleotide primers complementary to each strand of the polynucleotide. These primers also contain restriction enzyme cleavage sites to facilitate subcloning into a vector. Replicable vector components generally include, but are not limited to, one or more of the following: a signal sequence, an origin of replication, and one or more marker or selectable genes.

[0084] To express the polypeptide of the present invention, the nucleotide sequence encoding the polypeptide, or a functional equivalent, may be inserted into a suitable expression vector, i.e., a vector containing the necessary elements for the transcription and translation of the inserted coding sequence. Methods well known to those skilled in the art may be used to construct an expression vector containing a sequence encoding the polypeptide of interest and suitable transcription and translation control elements. These methods include in vitro recombinant DNA methods, synthetic methods, and in vivo genetic recombination. Such techniques are described, for example, in Sambrook, J., et al. (1989) Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press, Plainview, NY, and Ausubel, FM et al. (1989) Current Protocols in Molecular Biology, John Wiley & Sons, New York.NY.

[0085] The present invention also provides kits useful for carrying out diagnostic and prognostic assays using the antibodies, polypeptides and nucleic acids of the present invention. The kits of the present invention include a suitable container that contains the HIV antibodies, polypeptides or nucleic acids of the present invention, either in labeled or unlabeled form. In addition, when the antibodies, polypeptides or nucleic acids are provided in a labeled form suitable for indirect binding assays, the kits further include reagents for carrying out the appropriate indirect assays. For example, the kits may include one or more suitable containers that contain an enzyme substrate or a derivatizing agent, depending on the nature of the label. Control samples and / or instructions may also be included. The present invention also provides kits for detecting the presence of the HIV antibodies of the present invention or the nucleotide sequence of the HIV antibodies in biological samples by PCR or mass spectrometry.

[0086] As used herein, "label" refers to a detectable compound or composition that is directly or indirectly bound to an antibody to generate a "labeled" antibody. The label can also be bound to the polypeptides and / or nucleic acid sequences disclosed herein. The label can be detectable alone (e.g., a radioisotope label or a fluorescent label), or in the case of an enzyme label, can catalyze a chemical modification of a substrate compound or composition that is detectable. The antibodies and polypeptides of the described invention can also be modified to include an epitope tag or label, for example, for use in purification or diagnostic applications. Suitable means of detection include the use of labels such as, but not limited to, radioactive nucleotides, enzymes, coenzymes, fluorescent agents, chemiluminescent agents, chromogens, enzyme substrates or cofactors, enzyme inhibitors, cofactor complex, free radicals, particles, dyes, etc.

[0087] According to another embodiment, the present invention provides a diagnostic method. The diagnostic method generally includes contacting a biological sample collected from a patient, such as blood, serum, saliva, urine, sputum, cell swab sample or tissue biopsy material, with an HIV antibody, and determining whether the antibody selectively binds to the sample as compared to a control sample or a predetermined cut-off value, thereby indicating the presence of the HIV virus.

[0088] According to another embodiment, the present invention provides a method for detecting the presence of the HIV antibodies of the present invention in a biological sample from a patient. The detection method generally includes collecting a biological sample from a patient, such as blood, serum, saliva, urine, sputum, cell swab sample or tissue biopsy material, isolating the HIV antibodies or fragments thereof, or the nucleic acid encoding the HIV antibodies, and examining for the presence of the HIV antibodies in the biological sample. The present invention also provides a method for detecting the nucleotide sequence of the HIV antibodies in cells. The nucleotide sequence of the HIV antibodies may be detected using the primers disclosed herein. The presence of the HIV antibodies in a biological sample from a patient may be determined by known recombinant methods and / or the use of a mass spectrometer.

[0089] In another embodiment, the present invention provides a method for detecting an HIV antibody comprising a heavy chain comprising a highly conserved consensus sequence and a light chain comprising a highly conserved consensus sequence in a biological sample, the method comprising obtaining an immunoglobulin-containing biological sample from a mammalian subject, isolating an HIV antibody from the sample, and identifying the highly conserved consensus sequences of the heavy and light chains. The biological sample may be blood, serum, saliva, urine, sputum, a cell swab sample, or a tissue biopsy. The amino acid sequence may be determined by methods known in the art, including, for example, PCR and mass spectrometry.

[0090] The term "assessing" includes any form of measurement and includes determining whether an element is present or not. The terms "determining," "measuring," "evaluating," "assessing," and "assaying" are used interchangeably and include quantitative and qualitative determinations. Assessment can be relative or absolute. "Assessing the presence of" includes determining the amount of something present and / or determining whether it is present or absent. As used herein, the terms "determining," "measuring," and "assessing" and "assaying" are used interchangeably and include both quantitative and qualitative determinations.

[0091] II. Methods of Reducing Viral Replication Further provided are methods for reducing an increase in HIV viral titer, viral replication, viral growth, or the amount of HIV viral proteins in a subject. According to another embodiment, a method includes administering to a subject an HIV antibody in an amount effective to reduce an increase in HIV titer, viral replication, or the amount of HIV proteins of one or more HIV strains or isolates in the subject.

[0092] According to another embodiment, the present invention provides a method for reducing viral replication or reducing the spread of HIV infection to additional host cells or tissues, the method comprising contacting a mammalian cell with an antibody or portion thereof that binds to an antigenic epitope on gp120.

[0093] III. Treatment method According to another embodiment, the present invention provides a method for treating a mammal infected with a viral infection, such as HIV, comprising administering to said mammal a pharmaceutical composition comprising an HIV antibody as disclosed herein. According to one embodiment, said method for treating a mammal infected with HIV comprises administering to said mammal a pharmaceutical composition comprising an antibody or fragment thereof of the present invention. The composition of the present invention can comprise more than one antibody (e.g., a plurality of antibodies or a pool of antibodies) having the disclosed properties. The composition can also comprise other HIV neutralizing antibodies as known in the art, such as, but not limited to, VRC01, PG9 and b12.

[0094] Passive immunization has been proven to be an effective and safe strategy for the prevention and treatment of viral diseases. (See, e.g., Keller et al., Clin. Microbiol. Rev. 13:602-14 (2000); Casadevall, Nat. Biotechnol. 20:114 (2002); Shibata et al., Nat. Med. 5:204-10 (1999); and Igarashi et al., Nat. Med. 5:211-16 (1999)). Passive immunization using human monoclonal antibodies provides an emergency therapeutic strategy for the acute prevention and treatment of HIV.

[0095] A subject at risk for an HIV-related disease or disorder includes a patient who has been in contact with an infected person or who has been exposed to HIV in some other way. Administration of a prophylactic agent can occur prior to the manifestation of symptoms characteristic of an HIV-related disease or disorder in order to prevent or slow the progression of the disease or disorder.

[0096] For in vivo treatment of human and non-human patients, pharmaceutical formulations comprising the HIV antibodies of the invention are administered or provided to the patient. When used for in vivo therapy, the antibodies of the invention are administered to the patient in a therapeutically effective amount (i.e., an amount that eliminates or reduces the viral load of the patient). The antibodies are administered to human patients according to known methods, for example, intravenously, for example as a bolus or by continuous infusion over a period of time, intramuscularly, intraperitoneally, intracerebrospinal, subcutaneously, intra-articularly, intrasynovially, intrathecally, orally, topically or by inhalation routes. The antibodies can be administered parenterally, possibly at the site of target cells, or intravenously. In some embodiments, the antibodies are administered by intravenous or subcutaneous administration. The therapeutic compositions of the invention can be administered systemically, parenterally or locally to the patient or subject. The above parameters for assessing treatment success and disease improvement are easily measured by routine procedures well known to physicians.

[0097] For parenteral administration, the antibody may be formulated in a unit dosage form for injection (solution, suspension, emulsion) with a pharma- ceutically acceptable parenteral vehicle. Examples of such vehicles include, but are not limited to, water, saline, Ringer's solution, dextrose solution, and 5% human serum albumin. Non-aqueous vehicles include, but are not limited to, fixed oils and ethyl oleate. Liposomes can be used as carriers. The vehicle may also contain small amounts of additives, such as substances that improve isotonicity and chemical stability, such as buffers and preservatives. The antibody can be formulated in such vehicles at a concentration of about 1 mg / mL to 10 mg / mL.

[0098] The dosage and dosing schedule depend on various factors readily determined by a physician, such as the nature of the infection, e.g., its therapeutic index, the patient, and the patient's medical history. Generally, a therapeutically effective amount of the antibody is administered to the patient. In some embodiments, the amount of antibody administered ranges from about 0.1 mg / kg to about 50 mg / kg of patient body weight. Depending on the type and severity of the infection, an antibody of about 0.1 mg / kg to about 50 mg / kg (e.g., about 0.1 - 15 mg / kg / dose) of body weight is an initial candidate dosage for administration to the patient, e.g., by one or more separate administrations or by continuous infusion. The course of this treatment is readily monitored by conventional methods and assays and based on criteria known to physicians or other persons of ordinary skill in the art. The above parameters for assessing treatment success and disease improvement are readily measurable by routine procedures known to physicians.

[0099] Other therapies may be used in combination with the administration of the HIV antibodies of the invention. Combinations include co - administration using separate formulations or a single pharmaceutical formulation, and sequential administration in either order, where preferably there is a period during which both (or all) of the active agents exert their biological activity simultaneously. Such combination therapies can result in a synergistic therapeutic effect. The above parameters for assessing treatment success and disease improvement are readily measurable by routine procedures well - known to physicians.

[0100] The terms "treat" or "treatment" or "palliation" are used interchangeably and refer to both therapeutic treatment and prophylactic or preventative measures, where the purpose is to prevent or slow (alleviate) the targeted pathological condition or disorder. Those in need of treatment include those who already have the disorder, as well as those who are susceptible to the disorder or those who must be prevented from the disorder. A subject or mammal is successfully "treated" for an infection if, after receiving a therapeutic amount of the antibody according to the method of the present invention, the patient shows an observable and / or measurable reduction or absence of one or more of the following: a reduction in the number of infected cells or the absence of infected cells; a reduction in the percentage of total cells that are infected; and / or some relief of one or more of the symptoms associated with the particular infection; reduced morbidity and mortality, and improved quality of life issues. The above parameters for assessing successful treatment of a disease and improvement of a disease are easily measurable by routine procedures known to a physician.

[0101] The term "therapeutically effective amount" refers to an amount of an antibody or drug effective to treat a disease or disorder in a subject or mammal.

[0102] Administration "in combination with" one or more further therapeutic agents includes simultaneous (at the same time) and sequential administration in any order.

[0103] As used herein, a "carrier" includes a pharma- ceutically acceptable carrier, excipient, or stabilizer that is non-toxic to the cells or mammals exposed at the dosages and concentrations used. Often, the physiologically acceptable carrier is an aqueous pH buffered solution. Examples of physiologically acceptable carriers include buffers such as phosphate, citrate and other organic acids; antioxidants, including but not limited to ascorbic acid; low molecular weight (less than about 10 residues) polypeptides; proteins, such as but not limited to serum albumin, gelatin or immunoglobulins; hydrophilic polymers, such as but not limited to polyvinylpyrrolidone; amino acids, such as but not limited to glycine, glutamine, asparagine, arginine or lysine; monosaccharides, disaccharides and other carbohydrates, including but not limited to glucose, mannose or dextrins; chelating agents, such as but not limited to EDTA; sugar alcohols, such as but not limited to mannitol or sorbitol; salt-forming counterions, such as but not limited to sodium; and / or non-ionic surfactants, such as but not limited to TWEEN, polyethylene glycol (PEG) and PLURONICS.

[0104] Where a range of values ​​is given, it is understood that each intervening value between the upper and lower limits of that range, to one-tenth of the unit of the lower limit, and any other stated or intervening value in the stated range, is encompassed within the invention, unless the context clearly dictates otherwise. The upper and lower limits of these smaller ranges, which may be independently included in these smaller ranges, are also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where a stated range includes one or both limits, ranges excluding either of both of those included limits are also included in the invention. EXAMPLES

[0105] Example 1 This example describes the materials and methods used in Examples 2-5 below.

[0106] HIV antibodies were cloned and generated according to gp140-specific single B cell capture as previously described (Mouquet, H. et al. PLoS One 6, e24078 (2011); Tiller, T. et al. J Immunol Methods 329, 112-24 (2008); and Scheid, JF et al. Nature 458, 636-40 (2009)). GM and 10-1074 GM "Glycomutant" antibodies were generated by substituting residues 10-1074 at HC positions 32, 53, 54, 58, 97, 1001 with PGT121 and vice versa. The binding properties of anti-gp140-antibodies to HIV Env protein were analyzed by ELISA, SPR and glycan microarray assays as previously described (Scheid, JF et al. Science 333, 1633-7 (2011); Walker, LM et al. Nature 477, 466-70 (2011); and Mouquet, H. et al. PLoS One 6, e24078 (2011)). Neutralization was assessed using (i) a luciferase-based assay in TZM.bl cells and (ii) a PBMC-based assay using infection with primary HIV-1 variants as previously described (Li, M. et al. J Virol 79, 10108-25 (2005); Euler, Z. et al. Journal of virology 85, 7236-45 (2011); and Bunnik, EM et al. Nature medicine 16, 995-7 (2010)). The structures of PGT121 ('unliganded' and 'liganded'), 10-1074 and GL Fab fragments were solved by molecular replacement to 2.8 Å, 2.3 Å, 1.8 Å and 2.4 Å resolution, respectively.

[0107] Single B cell RT-PCR and Ig gene analysis gp140 from PBMCs of patient 10 (pt10; referred to as patient 17 in Nature 477(7365):466-470)+ CD19 + IgG + Single-cell sorting of B cells, cDNA synthesis, and nested PCR amplification of Ig genes were performed in a previous study (PLoS One 6(9):e24078). The Igλ genes expressed by the PGT121 clonal variants were amplified using a forward primer (L-Vλ3-21) further upstream within the leader region to avoid regions that may be subject to mutation (31). * 02:5'CTGGACCGTTCTCCTCCTCG 3'). All PCR products were sequenced and analyzed for Ig gene usage, CDR3 analysis and VH / Vκ somatic hypermutation counts (IgBLAST; http: / / www.ncbi.nlm.nih.gov / igblast and IMGT®; http: / / www.imgt.org). Multiple sequence alignments were performed using the MacVector program (v.12.5.0) with the ClustalW analysis function (default parameters) and were used to generate phylogenetic trees by the neighbor-joining method (best-tree and outgroup-rooted methods). Alternatively, phylogenetic trees were generated using the UPGMA method (best-tree mode).

[0108] Germline (GL) precursor gene segments of PGT121-like and 10-1074-like antibodies were identified using IgBLAST (http: / / www.ncbi.nlm.nih.gov / igblast) and IMGT® / V-QUEST (http: / / www.imgt.org / IMGT_vquest / share / textes / ). H 4-59 * 01, J H 6 * 03. V L 3-21 * 02 and J L 3 *02. (These gene segments are among the most frequently used in the human antibody repertoire (PLoS One 6(8):e22365; Immunogenetics 64(5):337-350). To construct a representative GL ancestral sequence, we aligned the IgH and IgL sequences of 10-996 (the antibody containing the least somatic hypermutations) to the GL sequence using IgBLAST (http: / / www.ncbi.nlm.nih.gov / igblast). The GL IgH sequence was derived from the mature V H and J. H The gene segments are replaced with their GL counterparts, and the N region nucleotides and D H The GL IgL sequence was constructed by using the 10-996 sequence for the CDRH3 region containing the gene segment. L 3-21 * 02 and J L 3 * 02 gene segment sequences.

[0109] Antibody cloning and production The purified and digested PCR products were then cloned into human Igγ 1 The IgH and Igλ genes were then sequenced and compared with the original PCR product sequences. PGT121 and 10-303 shared the same Igλ gene and had one amino acid difference at position 2 of the IgH gene (Figure 4); therefore, to produce PGT121 IgG, we used the 10-303Igλ and PGT121 IgH genes, which were generated by introducing a single substitution (V2M) into the 10-303IgH gene by site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit; Stratagene). To generate His-tagged Fab, we used the IgG1 C H 1 domain, followed by a 6x-His tag. 1PGT121 and 10-1074 V were prepared by modifying the vector (Science 301(5638):1374-1377). H The gene was subcloned into the 6xHis-IgCγ1 expression vector. GM (S32Y, K53D, S54R, N58T, H97R, T100lY) and 10-1074 GM The IgH DNA fragment encoding the (Y32S, D53K, R54S, T58N, R97H, Y1001T) mutant antibody was obtained as a synthetic minigene (IDT) and 1 It was subcloned into an expression vector.

[0110] 10-1074 GM The heavy chain sequences for are listed below with the mutations underlined. GM The light chain sequence of is the same as 10-1074. QVQLQESGPGLVKPSETLSVTCSVSGDSMNN S YWTWIRQSPGKGLEWIGYIS K.S. ESA N YNPSLNSRVVISRDTSKNQLSLKLNSVTPADTAVYYCATAR H GQRIYGVVSFGEFF T YYSMDVWGKGTTVTVSS.

[0111] Antibodies and Fab fragments were produced by transient transfection of IgH and IgL expression plasmids into exponentially growing HEK293T cells (ATCC, CRL-11268) using the polyethyleneimine (PEI)-precipitation method (PLoS One 6(9):e24078). IgG antibodies were affinity purified using Protein G Sepharose beads (GE Healthcare) according to the manufacturer's instructions. Fab fragments were affinity purified using HisPur™ Cobalt resin (Thermo scientific) as described below.

[0112] HIV-1 Env Protein Alanine mutations were introduced into the pYU-2 gp120 vector (a gift from J. Sodroski, Harvard Medical School) at positions 301 to 303 (Asn-Asn-Thr), 324 to 325 (Gly-Asp), and 332 (Asn) (HXBc2 amino acid numbering) using the QuikChange Site-Directed Mutagenesis kit (Stratagene) according to the manufacturer's instructions. The same procedure was used to introduce alanine mutations into the pYU-2 gp120 vector (a gift from J. Sodroski, Harvard Medical School) at positions 301 to 303 (Asn-Asn-Thr), 324 to 325 (Gly-Asp), and 332 (Asn) (HXBc2 amino acid numbering). N332A Asn262 in the vector gp120 and Asn406 gp120 A "double glycan" mutant was generated by introducing a single alanine mutation into each PNGS located between the . Site-specific mutations were confirmed by DNA sequencing.

[0113] YU-2 gp140 (Journal of virology 74(12):5716-5725), YU-2 gp120, HXB2c gp120 コア HEK 293T cells were transfected with expression vectors encoding the high mannose only YU-2 gp120 protein (gp120 kif ), 25 μM kifunesine (Enzo Life Sciences) was added at the time of transfection. Culture supernatants were harvested and concentrated using a centrifugation-based filtration device (Vivacell 100, Sartorius Stedim Biotech Gmbh) that allowed buffer exchange of the sample into 10 mM imidazole, 50 mM sodium phosphate, 300 mM sodium chloride; pH 7.4. Proteins were purified by affinity chromatography using HisPur™ cobalt resin (Thermo scientific) according to the manufacturer's instructions.

[0114] For the deglycosylation reaction, 50 μg of HEK 293T cell-produced YU-2 gp120 in PBS was incubated overnight at 37 °C with 200 U of PNGase F (New England Biolabs) or 10,000 U of Endo H in the respective reaction buffer without denaturant. f After buffer exchange into PBS using a centrifugal filter (Amicon® Ultra, Millipore), glycosidase-treated gp120 (200 ng) was examined by SDS-PAGE using 4–12% NuPAGE gels (Invitrogen) followed by silver staining (Pierce Silver Stain Kit, Thermo Scientific).

[0115] ELISAs High-binding 96-well ELISA plates (Costar) were coated overnight with 100 ng / well purified gp120 in PBS. After washing, plates were blocked for 2 h with 2% BSA, 1 μM EDTA, 0.05% Tween-PBS (blocking buffer) and then incubated for 2 h with IgG at a concentration of 26.7 nM (or 427.2 nM for ELISA using the YU-2 gp120 double glycan mutant), serially diluted 1:4 seven times in PBS. After washing, plates were developed by incubation for 1 h with goat-HRP-conjugated anti-human IgG antibody (Jackson ImmunoReseach) (at 0.8 μg / mL in blocking buffer) and by addition of HRP chromogenic substrate (ABTS solution, Invitrogen) (PLoS One 6(9):e24078). Selected gp120 V3 Antibody binding to the overlapping peptides was tested using the peptide-ELISA method described previously.

[0116] For competitive ELISAs, gp120-coated plates were blocked with blocking buffer for 2 hours and then incubated for 2 hours with biotinylated antibodies (at concentrations of 26.6 nM for PGT121, 0.21 nM for 10-1074, 0.43 nM for 10-996, and 1.67 nM for 10-1369) in 1:2 serial dilutions of antibody competitors in PBS (IgG concentration range of 5.2 to 667 nM). Plates were run as described above using HRP-conjugated streptavidin (Jackson ImmunoResearch) (at 0.8 μg / mL in blocking buffer). All experiments were performed at least in duplicate.

[0117] Glycan microarray analysis Microarrays were generated by robotically printing (Methods Mol Biol 808:117-136) lipid-linked glycan probes (neoglycolipids) at two levels (2 and 5 fmol / spot) in replicates onto nitrocellulose-coated glass slides. Binding assays were performed using microarrays containing 15 neoglycolipids derived from high mannose and complex-type N-glycans. The sequences of the probes are shown in Figure 7A. Briefly, antibodies were tested at 50 μg / mL and binding was detected with biotinylated anti-human IgG (Vector) followed by AlexaFluor 647-labeled streptavidin (Molecular Probes).

[0118] Surface plasmon resonance Experiments were performed using a Biacore T100 (Biacore, Inc) (Nature 467(7315):591-595). Briefly, YU-2 gp140 and gp120 proteins were primary amine coupled onto a CM5 chip (Biacore, Inc) at a coupling density of 300 RU. Anti-gp120 IgG and germline precursors (GL) were injected over the flow cell at 1 μM and 10 μM, respectively, at a flow rate of 35 μL / min with an association phase of 3 min and a dissociation phase of 5 min. The sensor surface was regenerated by a 30 s injection of 10 mM glycine-HCl pH 2.5 at a flow rate of 50 μL / min. Dissociation (k d (seconds -1 )), meeting (k a (M -1 seconds -1 ) and the binding constant (K D (M) or K A (M -1 ) was calculated from kinetic analysis after background subtraction using a 1:1 binding model without bulk reflection coefficient (RI) correction (Biacore T100 Evaluation software). The binding constant for bivalent IgG calculated using the 1:1 binding model was K D To emphasize that the values ​​include potential avidity effects, they are referred to in this document as "apparent" affinities.

[0119] Neutralization assay Virus neutralization was assessed using a luciferase-based assay in TZM.bl cells (J Virol 79(16):10108-10125). HIV-1 pseudoviruses tested contained mainly tier-2 and tier-3 viruses (Journal of virology 84(3):1439-1452) (Tables 4 and 5). High-mannose only pseudoviruses were neutralized in wild-type cells (Enzo Life Sciences) treated with 25 μM kifunesine (Figure 8C) or HEK293S GnTI cells (Figure 8D). - / - Nonlinear regression analysis was used to determine the concentration at which half-maximal inhibition was observed (IC 50Neutralizing activity was also assessed using previously characterized PBMC-based assays (Journal of Virology 85(14):7236-7245; Nat Med 16(9):995-997) using infections with primary HIV-1 variants (n=95) isolated from clade B-infected donors with known seroconversion dates between 1985 and 1989 ("past seroconverters", n=14) or clade B-infected donors with known seroconversion dates between 2003 and 2006 ("current seroconverters", n=21). Neutralizing activity of each antibody was measured as the percentage of virus neutralized using IC values ​​ranging from 0.001 to 50 μg / mL. 50 The area under the best-fit curve was calculated using GraphPad Prism software (v5.0b) fitting the values. Relative area under the curve (RAUC) values ​​were derived by normalizing all AUC values ​​by the highest value (obtained at 10-1074).

[0120] statistical analysis Statistical analysis was performed with GraphPad Prism software (v5.0b). The apparent binding affinity of antibodies to gp120 and gp140 versus neutralizing potency in the TZM-bl assay against a selected panel of nine virus strains was analyzed using the Spearman correlation test. The Mann-Whitney test was used to compare (i) the affinity of antibodies belonging to the PGT121 or 10-1074 groups to gp120 / gp140 and (ii) the neutralizing activity against viruses isolated from past and current seroconverters.

[0121] Crystallization and structure determination We expressed 6x-His tagged PGT121, 10-1074 and 10-996GL Fabs for crystallization. The Fabs were extracted from the supernatant of transiently transfected HEK293-6E cells by sequential NiCl extraction. 2+Crystals of the ligand-free PGT121 Fab were prepared by protein A affinity chromatography (Pierce) and papain digestion of the IgG to obtain the Fab fragment, which was further purified by size-exclusion chromatography on Superdex200 10 / 300 (GE Healthcare).

[0122] Purified Fab was concentrated to 8-20 mg / mL in PBS buffer ('liganded' PGT121, 8 mg / mL; 10-1074 and GL, 20 mg / mL). 'Ligand' PGT121 Fab crystals were prepared from protein samples (final concentration: 15 mg / mL) mixed with a 3-fold molar excess of NA2 glycan and incubated for 2 h at 20 °C. Crystallization conditions were screened on a Mosquito® crystallization robot (TTP labs) in 400 nL droplets with a 1:1 protein to reservoir ratio at 20 °C. Crystals of 'liganded' PGT121 Fab (P2 1 2 1 2 1 ;a=56.8, b=74.7, c=114.9Å), 24%PEG 4,000, 0.1M Tris-HCl pH8.5, 10mM CuCl 2 The crystals of the “liganded” PGT121 Fab (P2 1 2 1 2 1 ;a=67.8, b=67.8, c=94.1Å), 17%PEG 10,000, 0.1M Bis-Tris pH5.5, 0.1M CH 3 COOHNH 4 The crystals of 10-1074 Fab (P2 1 ; a = 61.4, b = 40.3, c = 84.5 Å; β = 95.39°) were obtained in 25% PEG 3,350, 0.1 M Bis-Tris pH 5.5, 0.2 M NaCl, and crystals of GL Fab (P21 ; a = 54.9, b = 344.7, c = 55.2 Å; β = 91.95°) in 20% PEG 3,350, 0.24 M sodium malonate pH 7.0, 10 mM MnCl 2 Crystals were cryoprotected by immersion in mother liquor containing 20% ​​glycerol (unliganded and liganded PGT121 Fab) or 20% ethylene glycol (10-1074 Fab and GL Fab), followed by flash cooling in liquid nitrogen.

[0123] Diffraction data were collected at beamline 12-2 (wavelength = 1.029 Å) using a Pilatus 6 Mpixel detector (Dectris) at the Stanford Synchrotron Radiation Lightsource (SSRL). Data were indexed, integrated, and scaled using XDS. Using data from the "ligand-free" PGT121 Fab crystal, we used Phenix to find two search models after removing residues in the CDRH3 and CDRL3 loops: C1 of PGT128 Fab, C2 of PGT128 Fab, and C3 of PGT128 Fab. H -C L domain (PDB code 3PV3) and V of 2F5 H -V L We used the domain (PDB code 3IDJ) to find molecular replacement solutions for one Fab per asymmetric unit (chains H and L for heavy and light chains, respectively). We then used the "unliganded" PGT121 structure as a search model to find molecular replacement solutions for the "liganded" PGT121 Fabs (one Fab per asymmetric unit), 10-1074 Fab (one Fab per asymmetric unit) and GL (four Fabs per asymmetric unit).

[0124] Iterative refinement (including non-crystallographic symmetry restraints for GL) was performed using Phenix, and models were manually fitted to electron density maps using Coot. Atomic models were fitted to 3.0 Å resolution (R work=21.6%, R free = 26.4%), and for 10-1074 Fab, 1.9 Å resolution (R work =18.7%, R free = 22.3%) and 2.4 Å resolution (R work =19.4%, R free = 23.7%) and for the "liganded" PGT121 Fab at 2.4 Å resolution (R work =20.1%, R free = 24.9%). The atomic model of PGT121 Fab contains 95.2%, 4.9% and 0.0% of residues within the favorable, allowed and disallowed regions of the Ramachandran plot, respectively (10-1074 Fab: 98.8%, 0.9%, 0.2%; GL Fab: 96.0%, 3.8%, 0.23%; PGT121 Fab with ligand: 96.7%, 3.1%, 0.2%). PyMOL was used for molecular visualization and to generate diagrams of the Fab structure. Calculations of buried surface area were performed with Areaimol (CCP4 suite) using the 1.4 Å probe.

[0125] Fab structures were aligned using the Super script in PyMOL. Pairwise Cα alignments were performed using PDBeFold.

[0126] Example 2 Predominance and diversity of PGT121 clonotypes We isolated gp140-specific IgG memory B cells from clade A-infected African donors using YU-2 gp140 trimers as "bait". We identified 87 matched immunoglobulin heavy (IgH) and light (IgL) chain genes corresponding to 23 unique clonal families. One clonal family dominated the IgH anti-gp140 repertoire, representing approximately 28% of all expanded B cell clones. This B cell family corresponds to the same clone as PGT121-123 (Nature 477(7365):466-470) and contained 38 members, 29 of which were unique variants at the nucleotide level (Table 3). Based on their IgH nucleotide sequences, the PGT121 family is divided into two groups: a PGT121-like group containing PGT121-123 and nine closely related variants, and a second group, 10-1074-like, containing 20 members. Although our previous primers (J Immunol Methods 329(1-2):112-124; Science 301(5638):1374-1377) did not amplify IgL genes expressed by PGT121B cell clones due to nucleotide deletions in the regions encoding framework region 1, we obtained 24 of 38 Igλ genes using new Igλ-specific primers designed to amplify highly somatically mutated genes (Table 3). Consistent with the high hypermutation level of IgH genes (18.2% of VH genes on average), the amplified Igλ genes were highly mutated (18.2% of Vλ genes on average) and had nucleotide deletions (12 to 21 nucleotides) in framework region 1 (FWR1) and 9 nucleotide insertions in framework region 3 (FWR3) (Figure 3B and Table 3).

[0127] Sequence alignments of three PGT antibodies (PGT-121, -122, and -123), eleven PGT121 and 10-1074 clonal variants (10-259, 10-303, 10-410, 10-847, 10-996, 10-1074, 10-1121, 10-1130, 10-1146, 10-1341, 10-1369, and 10-1074GM,), putative germline (GL), and consensus sequences are shown in Figures 3(a) and 3(b). Sequences for the corresponding heavy chain variable regions, light chain variable regions, heavy chain CDRs, and light chain CDRs according to both the IMGT and KABT systems are listed in Table 1 below. Sequence identification numbers assigned to the sequences according to the KABT system are listed in Table 2 below.

[0128] [Table 1-1]

[0129] [Table 1-2]

[0130] [Table 1-3]

[0131] [Table 1-4]

[0132] [Table 2]

[0133] Eleven novel unique mutants were expressed (Table 3) and demonstrated binding to YU-2 gp120 and gp140 by ELISA and surface plasmon resonance (SPR). Unless otherwise noted, gp120 and gp140 proteins for these and other experiments were expressed in mammalian cells capable of binding either complex or high mannose N-glycans to PNGS. The level of reactivity with gp120 differed between antibodies belonging to the PGT121 and 10-1074 groups, with the latter exhibiting a higher apparent affinity (Figure 3A), primarily due to slower dissociation from gp120 / gp140 for the 10-1074 analogue antibody (Figure 4B).

[0134] Example 3 PGT121 and 10-1074 epitopes Asn332 near the V3 loop stem gp120 It has been reported that V3 is crucial for binding and virus neutralization by PGT121 (Nature 477(7365):466-470), therefore, we investigated the role of V3 in antigen recognition by PGT121-like and 10-1074-like antibodies. コア ) or the HXB2 gp120 "core" protein, which retains a portion of V3 (2CC-core), and the YU-2 gp120 mutant protein with a double alanine substitution in the V3 stem (gp120 GD324-5AA ELISA was performed using the same antibody as in Example 1. The antibodies tested were those that bind to the mutant lacking the V3 loop compared to intact YU-2 gp120 and gp120 GD324-5AA Both groups of antibodies showed reduced reactivity to V3, with binding of the 10-1074 group antibodies being the most affected (Figure 5A and B). These results suggest that recognition by both groups of antibodies requires protein determinants in the vicinity of the V3 loop. None of the antibodies bound to overlapping peptides that span V3, suggesting that the target epitope is discontinuous and / or requires a specific conformation that is not achieved by the isolated peptides (Figure 5C).

[0135] Asn332 gp120(Previous numbering (J Proteome Res 7(4):1660-1674)) gp120 ) is the N-terminal residue of a potential N-glycosylation site (PNGS) defined as the sequence Asn-X-Ser / Thr. gp120 To determine whether YU-2gp120 and / or its N-linked glycans are required for gp120 reactivity of the novel PGT121 and 10-1074 group antibodies, we identified YU-2gp120 and its N-linked glycans by ELISA. N332A The N332A substitution reduced binding of PGT121 and all of the novel antibody variants, but not of a mutant gp120 lacking the adjacent glycosylation site (gp120 NNT301-3AAA Their reactivity to the Asn332 mutant was unchanged. gp120 To determine whether PNGS, in addition to PNGS, affects recognition by novel antibodies, we compared the N332A mutation in YU-2 gp120 with Asn262. gp120 and Asn406 gp120 A series of 11 double glycan mutants were constructed combining mutations in the intervening PNGS sites. All of the PGT121-like and 10-1074-like antibodies were N332A The glycan mutants bound to each of the dual glycan mutants with comparable affinity for .

[0136] To compare total glycan recognition by PGT121-like and 10-1074-like antibodies, we investigated their binding to YU-2 gp120 treated with PNGase F, which cleaves both complex and high-mannose N-glycans. Because gp120 cannot be completely deglycosylated enzymatically unless it is denatured, PNGase F treatment resulted in partial deglycosylation of natively folded gp120 (Figure 6). Nevertheless, the reactivity of the two groups of antibodies differed in that partial deglycosylation of gp120 by PNGase F reduced the binding activity of all PGT121-like antibodies, but not any of the 10-1074-like antibodies (Figure 6C). Similar experiments performed with YU-2 gp120 treated with Endo H, which cleaves high-mannose N-glycans but not complex-type N-glycans, affected binding of the 10-1074-like antibody more than the PGT121-like antibody (Fig. 6D ).

[0137] N-glycan microarrays revealed that six of seven tested PGT121-like antibodies showed detectable binding to complex mono- or biantennary N-glycans terminated with galactose or α2-6-linked sialic acid, but no detectable binding to high mannose type glycans. This is consistent with the lack of binding of PGT121-123 to high mannose N-glycans, as well as the lack of Mann binding for gp120 binding. 4 and Man 9 This confirms and extends previous reports of a lack of competition by the dendrons (Figure 7). In contrast, there was no detectable binding to protein-free glycans by the 10-1074-like antibody (Figure 7). PGT121-like antibodies bound to protein-free complex N-glycans and not to high mannose N-glycans, whereas PGT121-like antibodies bound to YU-2 gp120 (gp120) produced in cells treated with the mannosidase inhibitor kifunesin, which results in exclusive binding of high mannose glycans to PNGS. kif ) (Fig. 8B). The majority of PGT121-like antibodies bound to gp120 kifOn the other hand, the 10-1074-like antibody exhibited a small but reproducible decrease in binding to gp120. kif The full binding to PGT121 was maintained (Figure 8B). These results are consistent with the hypothesis that not only high-mannose N-glycans but also complex-type N-glycans may contribute to the epitope of PGT121-like antibodies.

[0138] Epitope mapping experiments were performed by competitive ELISA using two representative members of each group (PGT121 and 10-1369 for the PGT121-like group; 10-1074 and 10-996 for the 10-1074-like group). All four antibodies showed cross-competition, but PGT121 inhibited the binding of 10-996 and 10-1074 to gp120 more slowly than vice versa. To further map the target epitopes, we used anti-gp120 antibodies that recognize the crown of the V3 loop (Figure 5), the CD4bs, the coreceptor binding site (CD4-induced; CD4i), a group of high-mannose N-glycans (2G12) (Journal of Virology 76(14):7293-7305; Proc Natl Acad Sci USA 102(38):13372-13377)) or the V3 loop and N-linked glycans at positions 301 and 332 (PGT128). The anti-V3 crown antibodies inhibited the binding of PGT121 and 10-1369, but did not interfere with the binding of 10-996 and 10-1074. PGT128, and to a lesser extent 2G12, reduced the binding of all four antibodies to gp120, whereas the CD4bs and CD4i antibodies did not.

[0139] Taken together, these data suggest that the PGT121 clone members contain a sequence similar to that of the V3 loop and Asn332. gp120 This suggests that they recognize sites containing protein determinants in the vicinity of related glycans. However, the clones fall into two families, the PGT121-like and the 10-1074-like, that differ in their affinity for gp120 and the role of the glycans in epitope formation.

[0140] Example 4 Broad and potent HIV neutralization To evaluate the neutralizing activity of the novel PGT121 mutants, we measured their ability to inhibit HIV infection of TZM-bl cells using 10 virus strains, including R1166.c1, which lacks the PNGS at gp120 position 332. All PGT121 mutants, including the 10-1074-like antibody, neutralized nine pseudoviruses and none of them neutralized the R1166.c1 control (Figure 1A and Table 4). Neutralizing activity correlated with affinity for the HIV spike, with the 10-1074 group showing slightly greater potency than the PGT121 group (Figure 1B and Figure 4C). A representative germline version (GL) of the PGT121 / 10-1074 antibody clonotype was unable to bind gp120 / gp140 or neutralize any of the viruses in the panel, implying that somatic mutations are required for binding and neutralization. Pairing of the GL light chain with mutant 10-1074- or 10-996-group heavy chains was unable to rescue binding or neutralization, suggesting that both mutant chains contribute to the correct assembly of the antibody paratope.

[0141] The following assays were performed to compare the neutralizing activity of PGT121 with that of two 10-1074-like antibodies (10-996 and 10-1074) against an extended panel of 119 difficult-to-neutralize pseudoviruses (classified as tier-2 and tier-3) (Tables 4 and 5). 10-996 and 10-1074 showed similar neutralizing potency and breadth to PGT121 (Figure 1C, Figure 9 and Tables 5 and 6). As expected, the majority of viruses with amino acid changes at gp120 positions 332 and / or 334 (spanning Asn332-X-Ser334 / Thr334PNGS) were resistant to neutralization (83.8% resistant to PGT121 and 100% resistant to 10-1074 and 10-996). The majority of viruses resistant to neutralization (68.5% for 10-996, 72.5% for 10-1074, and 60.8% for PGT121) were due to mutations in this PNGS (Table 7). Comparable neutralizing activity was observed for the IgG and Fab forms of PGT121 and 10-1074, suggesting that bivalency is not important for their activity (Figure 1D).

[0142] To assess the potential role of complex-type N-glycans on the HIV envelope in neutralization by PGT121 and 10-1074, we produced high-mannose-only virions in two different ways: by assembly of pseudoviruses in cells treated with kifunesine, resulting in Man 9 GlcNAc 2 Methods for obtaining N-linked glycans or HEK293S GnTI - / - As a result of the construction of Man in cells 5 GlcNAc 2 How N-linked glycans are derived. We found that PGT121 neutralized two of the three kifunesine-derived PGT121-sensitive / 10-1074-resistant strains equivalently to their counterparts produced in wild-type cells (Figure 8C). - / -Two PGT121-sensitive / 10-1074-resistant virus strains produced in cells were sensitive to PGT121 and 10-1074 equivalently to their counterparts produced in wild-type cells. Consistent with previous reports that complex N-glycans protect to some extent the CD4-binding site from antibody binding, GnTI - / - Viruses produced in cells were more sensitive to CD4-binding site antibodies (NIH45-46 G54W and 3BNC60) (Figure 8D).

[0143] Example 5 Novel Transmitted HIV-1 Next, we investigated the activity of PGT121 and 10-1074 against transmitted founder viruses by evaluating neutralization in a peripheral blood mononuclear cell (PBMC)-based assay using 95 clade B viruses isolated from cohorts of individuals who seroconverted between 1985 and 1989 ("past seroconverters", n = 14) or between 2003 and 2006 ("current seroconverters", n = 25) (51, 52). We compared PGT121 and 10-1074 to anti-CD4bs bNAbs and other bNAbs (including VRC01, PG9 / PG16, b12, 2G12, 4E10, and 2F5). Cluster analysis of neutralization activity showed separation into two groups; the PGT121 / 10-1074 group contained the most potent HIV neutralizers, including anti-CD4bs and PG9 antibodies (Table 8). Surprisingly, 10-1074 showed very good neutralizing potency against this clade B virus panel, presenting the largest breadth (67% of 95 clade B viruses) at 0.1 μg / mL for all bNAbs tested (Table 8). 10-1074 showed higher potency (~20-fold difference) than PGT121 against current clade B viruses, but both antibodies were more effective against past viruses than current viruses (Figure 1E and Figure 10).

[0144] Example 6 Crystal Structures of PGT121, 10-1074, and GL To investigate the structural determinants of the differences between PGT121-like and 1074-like antibodies, we solved the crystal structures of the Fab fragments of PGT121, 10-1074, and a representative germline precursor (GL) at 3.0 Å, 1.9 Å, and 2.4 Å resolution, respectively (Table 9). H and V L ) revealed that differences relative to GL were limited to small displacements in the CDRH3 and CDRL3 loops of the affinity matured Fab, with conservation of the main-chain structure (Table 10).

[0145] An unusual feature shared by antibodies is their long (25 residues) CDRH3 loop, which is located at the V H It forms a two-stranded antiparallel β-sheet that extends domains F and G. In each Fab, the tip of the extended CDRH3 loop contains primarily non-polar residues. Similar structural features were observed for the CDRH3 of PGT145, a carbohydrate-sensitive antibody whose epitope contains the gp120 V1V2 loop. However, the extended two-stranded β-sheet of CDRH3 of PGT145 contains primarily negatively charged residues, including two sulfated tyrosines at the tip. The V of PGT121 and PGT145 H -V L The alignment of (Table 10) is PGT145 Previously, CDRH3 PGT121 Extending the tip and V H The domains are aligned, but CDRH3 of PGT121, 10-1074 and GL are V L V L CDRH3 for PGT121 / CDRH3 10-1074 / CDRH3 GL The slope of opens a cleft between CDRH2 and CDRH3, a feature not shared by related antibodies.

[0146] PGT121 and 10-1074 are highly divergent from GL and from each other (out of 132 residues, PGT121 VH is 10-1074VH and G.L. VH and 10-1074 differ by 36 and 45 residues, respectively. VH and G.L. VH The majority of these PGT121 / 10-1074 differences are due to the CDR VH Interestingly, six substitutions in CDRH3 (residues 100d, 100f, 100h, 100j, 100l, 100n) alternate such that every second residue is substituted, resulting in a V L The cleft between CDRH2 and CDRH3 resurfaces due to the tilt of CDRH3 toward the nucleus. This region is likely to contribute to the different fine specificities of PGT121 and 10-1074. HC Five other solvent-exposed substitutions in GL (residues 64, 78, 80-82; strands D and E) are potential antigen contact sites, given that the framework regions in HIV antibodies can contact gp120. Other differences that may contribute to subtle specificity differences include Asp56, which is absent in 10-1074 or GL. HC Negative patches on PGT121 near the HC ), as well as positive patches on CDRL1 and CDRL3 that are not found on the analogous surface of GL.

[0147] Somatic mutations common to PGT121 and 10-1074 may be responsible for the shared characteristics of their epitopes. The heavy chains of PGT121 and 10-1074 share only three common mutations (out of 36 PGT121-GL differences and 29 10-1074-GL differences). In contrast, PGT121 and 10-1074 share 18 common light chain mutations (out of 37 PGT121-GL differences and 36 10-1074-GL differences), including an insertion in light chain FWR3 that causes a bulge in the loop connecting chains D and E, and a CDRL2 mutation that results in a low negatively charged surface. GL Asp50 in LC -Asp51 LCAsn50 in both PGT121 and 10-1074 LC -Asn51 LC The substitution is LC PGT121 and L.C. 10-1074 The majority of common substitutions (approximately 50% of the LC substitutions) introduced into CDRL1, CDRL2 and FWR2 are potential contact regions for the epitope shared by PGT121 and 10-1074. LC points out.

[0148] Next, Asn332 gp120 Bond and Asn301 gp120 Comparison was made with the structure of PGT128, which recognizes the linked glycan as well as V3 and was solved in complex with the ectodomain / mini-V3 loop gp120 expressed in cells unable to produce complex-type N-glycan modified proteins. Unlike the CDRH3 loop of PGT121 and 10-1074, PGT128 CDRH3 is PGT128 VL It is not leaning towards CDRH3. PGT128 does not contain a two-stranded β-sheet. In addition, CDRH3 PGT128 (18 residues) is shorter than the CDRH3 of PGT121 and 10-1074 (24 residues), but the CDRH2 PGT128 contains a six-residue insertion that is not found in PGT121 or 10-1074. Because of these differences, CDRH2 is the most prominent feature in PGT128, whereas CDRH3 is the most prominent feature in PGT121 and 10-1074. PGT128 and CDRL3 PGT128 Together, Asn332 gp120 Man bound to 8 / 9 Recognizes CDRH3 PGT128 makes contacts with the base of the V3 loop. This mode of gp120 recognition is not possible for PGT121 and 10-1074 because the structural features of their CDRH2 and CDRH3 loops differ significantly from those of PGT128, consistent with the ability of PGT128, but not PGT121 and 10-1074, to recognize protein-free high-mannose glycans (Figure 7).

[0149] Example 7 Crystal structure of PGT121-glycan complex The 2.4 Å resolution structure of PGT121 with a complex sialylated biantennary glycan was solved (Table 9) using crystals obtained under conditions containing NA2, a complex asialyl biantennary glycan (Figure 7). Surprisingly, the glycan bound to PGT121 in our crystal structure is not NA2, but rather a complex N-glycan from the adjacent PGT121 Fab in the crystal lattice, specifically Asn105. HC The identity of this glycan was an N-glycan linked to Asn105. HC This is evident because there is electron density for the glycosidic linkage to PGT121 and for the terminal sialic acid on the Manα1-3Man antenna (the galactose and sialic acid moieties of the Manα1-6Man antenna were unresolved). The composition of the linked glycan corresponds to the portion of the α2-6 sialylated A2(2-6) glycan bound by PGT121 in the microarray experiment (Figure 7) and to the predicted sialyl linkages in the complex-type N-glycans bound to PNGS on proteins expressed in HEK293T cells. The V H -V L The domain is V of the PGT121 structure without the attached N-glycan ("unliganded" PGT121). H -V L The domains overlap without significant difference (Fig. 10), but the elbow bend angle (V H -V L and C. H 1-C L The angle between the pseudo-dyads differs between the structures, likely reflecting the flexibility that allows the Fab to adopt elbow bend angles that can vary depending on the crystal lattice forces.

[0150] Given that binding of complex-type N-glycans was observed in one crystal structure (the "liganded" PGT121 structure) but not in another (the "unliganded" PGT121 structure), we speculate that the affinity of PGT121 for complex-type N-glycans not bound to gp120 is within the range of the concentration of PGT121 in the crystals (~10 mM). The K for binding isolated glycans D But, Man 5 GlcNAc 2 1.6 mM K obtained for PG9 binding to -Asn D Assuming that the K for PGT121 binding of isolated glycans is in the range of 1–10 mM, which is comparable to D represents only a small contribution to the affinity of PGT121 for gp120, which is in the nM range (FIG. 4A).

[0151] The glycans in the "liganded" PGT121 structure are V H domain and makes extensive contacts with residues in all three CDRs (PGT121 HC Top buried surface area = 600 Å 2 The contacts include 10 direct hydrogen bonds and 18 water-mediated hydrogen bonds (Figure 11) with nine amino acids, anchoring the glycan between the N-acetylglucosamine moiety attached to the mannose at the branch site and the terminal sialic acid on the 1-3 antenna. Asp31 HC In addition to the water-mediated hydrogen bond with PGT121 residue Asp31 HC and His97 HCSeveral contacts with PGT121 are made by this sialic acid, including three direct hydrogen bonds with . Sialic acids also contribute to the water-mediated intraglycan hydrogen bond network. Direct contacts with sialic acids could explain the stronger binding of PGT121 to sialylated A2(2-6) glycans than to nonsialylated NA2 glycans in our glycan microarray analysis (Figure 7). Extensive water-mediated protein contacts established by the N-acetylglucosamine and galactose moieties of the 1-3 antenna could explain the observed binding to PGT121 for nonsialylated mono- and biantennary glycans (Figure 7).

[0152] Six of the residues that contribute direct or likely amino acid side chain contacts to the glycan (Ser32 HC-CDRH1 , Lys53 HC-CDRH2 , Ser54 HC-CDRH2 , Asn58 HC-CDRH2 , His97 HC-CDRH3 , Thr100l HC-CDRH3 ) is 10-1074 (Tyr32 HC-CDRH1 , Asp53 HC-CDRH2 , Arg54 HC-CDRH2 , Thr58 HC-CDRH2 , Arg97 HC-CDRH3 , Tyr100l HC-CDRH3 ), which is highly conserved among PGT121-like antibodies but not among 10-1074-like antibodies. The 10-1074 residues lack corresponding functional groups to make the observed glycan contacts or have bulky side chains that would cause steric clashes. Four of these residues are distinct from those in GL (Tyr32 HC-CDRH1 , Tyr53 HC-CDRH2 , Gln97 HC-CDRH3 , Tyr100l HC-CDRH3 ), which suggests that the lack of binding of 10-1074-like antibodies and GL to protein-free complex glycans in our glycan microarrays may be due to hydrogen bonding and / or steric clashes (e.g., Arg97 10-1074 On the other hand, His97 PGT121 ;Tyr100l 10-1074 In contrast, Thr100l PGT121Like most of the sequence differences between PGT121 and the 10-1074 group in the CDRH loops, and especially for the surface of the cleft between CDRH2 and CDRH3 where we observed bound complex-type N-glycans, differential recognition of complex-type glycans on gp120 may account for some or all of the observed differences in their fine specificity.

[0153] Example 8 Substitution of Glycan-Containing Antibody Residues Affects Neutralization To assess the contribution of complex-type N-glycan-containing residues identified from the “liganded” PGT121 structure, we generated two mutant antibodies designed to swap complex-type glycan-containing residues between PGT121 and 10-1074, i.e., 10-1074 IgG with six substitutions at PGT121 residues (IgH Y32S, D53K, R54S, T58N, R97H, Y100lT) and PGT121 IgG with reciprocal substitutions. GM and PGT121 GM ) displayed near wild-type apparent affinity for YU-2 gp120 / gp140 as measured by SPR (Figure 2A), demonstrating that the substitutions did not disrupt binding to envelope spikes from virus strains neutralized by both PGT121 and 10-1074 (Figure 1A). The ability to accommodate PGT121 complex-type N-glycan-containing residues into the 10-1074 background without disrupting binding to gp120 / gp140 bound by both wild-type antibodies implies that antigen binding is generally similar despite subtle specificity differences.

[0154] Unlike wild-type PGT121, PGT121 GMshowed no glycan binding in microarray experiments. This confirms that the substituted residues at position 10-1074 are not compatible with protein-free glycan binding (Figure 2B) and supports the suggestion that residues in contact with glycans in the "liganded" PGT121 structure are involved in the recognition of complex glycans in the microarray. GM also showed no binding to protein-free glycans (Fig. 2B), indicating that residues other than those substituted are involved in generating binding sites for protein-free complex-type N-glycans.

[0155] We next compared the neutralization of wild-type and "glycomutant" antibodies using a TZM-bl-based assay. We tested 40 virus strains, including strains with different resistance to PGT121 or 10-1074 as well as strains sensitive to both wild-type antibodies (Figure 2C and Table 12). GM and 10-1074 GM Consistent with binding of PGT121, both mutants neutralized YU-2 virus, but 64% of the PGT121-susceptible strains were neutralized by PGT121. GM (Figure 2C and Table 12). This suggests that the glycan-containing residues identified in the "liganded" PGT121 structure are relevant to the neutralizing activity of PGT121. Conversely, 10-1074 GM exhibited higher average potency against 10-1074-susceptible strains than wild-type 10-1074, including a greater than 3-fold increase in potency against four 10-1074-susceptible strains (WITO4160.33, ZM214M.PL15, Ce1172_H1, and 3817.v2.c59) (Figure 2C and Table 12). In general, substitution of PGT121 with 10-1074 resulted in a greater potency than 10-1074 in PGT121-susceptible / 10-1074-resistant strains. GM Although two of these strains (CNE19 and 62357_14_D3_4589) do not confer susceptibility to 10-1074 GM (IC 50= 0.19 μg / mL and 40.8 μg / mL). Interestingly, these were gp120 This is the only PGT121-susceptible / 10-1074-resistant strain that contains combined PNGS. The other PGT121-susceptible / 10-1074-resistant strains have Asn332 gp120 Lacking the linking glycan and PGT121 GM and 10-1074 GM resistant to 10-1074 for one strain (CNE19), implying that their susceptibility to wild-type PGT121 requires compensation by adjacent N-glycans and / or the protein portion of the epitope. GM This result was observed only for 10-1074 versus 10-1074 susceptible strains. GM Together with the general improvement observed for 10-1074 (Figure 2C), this is consistent with the interpretation that crystallographically identified glycan-containing residues may transfer PGT121-like recognition properties to 10-1074 in some circumstances and / or affect its potency in other circumstances. GM The loss of neutralizing activity of PGT121 provides evidence that the neutralizing activity of PGT121 requires residues identified in the "liganded" PGT121 structure as contacting complex-type N-glycans.

[0156] result PGT121 is a glycan-dependent bNAb originally identified in the serum of clade A infected donors in a functional screen that gave rise to only two clonally related members. Using gp140 trimers as "bait" for single cell sorting, 29 new clonal variants were isolated. The PGT121 clonal family contains distinct groups of closely related antibodies, the PGT121 group and the 10-1074 group. The results show that the epitopes of both groups are located at Asn332. gp120The PGT121-like and 10-1074-like antibody groups differ in amino acid sequence, gp120 / gp140 binding affinity, and neutralizing activity, with 10-1074-like antibodies showing a strong neutralizing effect at Asn332. gp120 Although PGT121-like antibodies are completely dependent on intact PNGS in gp120 It was able to neutralize some virus strains lacking PNGS.

[0157] A notable difference between the two antibody groups is that PGT121-like antibodies bound complex N-glycans in the carbohydrate array, whereas 10-1074-like antibodies showed no detectable binding to any of the protein-free N-glycans tested (Figure 7). Protein-free glycan binding by anti-HIV antibodies is not always detected; for example, PG9 recognizes gp120-bound high mannose glycans, but binding to any protein-free glycans was not detected in the microarray. Thus, a positive result in the glycan microarray implies engagement of a specific glycan with the antibody epitope, whereas a negative result does not exclude glycan recognition. For example, high mannose glycans can engage the PGT121 epitope, even if not detectable in the glycan microarray experiment, which is consistent with binding and neutralization of the high mannose-only forms of gp120 protein and virions (Figure 8).

[0158] The molecular basis for the differences between PGT121, 10-1074 and their GL precursors was partially revealed by their crystal structures. The finding that the majority of light chain somatic mutations are shared between PGT121 and 10-1074, while the heavy chain mutations are distinct, suggests that the light chains contact a shared portion of the gp120 epitope and the heavy chains recognize distinct features. All three of these antibodies display an extended CDRH3 with a non-polar tip that may allow access of cryptic epitopes. The differences in the antigen-binding sites of the two mature Fabs were mainly localized to the cleft between CDRH2 and the extended CDRH3. Interestingly, the putative antigen-binding cleft between CDRH2 and CDRH3 was also found in representative germline precursors of PGT121 and 10-1074.

[0159] V H Structural information on glycan recognition by PGT121-like antibodies was obtained from the crystal structure in which complex sialylated N-glycans attached to domain residues interact with the binding site of the adjacent PGT121Fab. Several features of the "liganded" PGT121 structure suggest that it is suitable for understanding the recognition of complex N-glycans on gp120 by PGT121-like antibodies. First, the glycan in the structure corresponding to the α2-6 sialylated glycan A2(2-6)PGT121 is bound in the microarray (Figure 7). Second, the glycan interacts with PGT121 using the cleft between CDRH3 and CDRH2, which structural analysis suggested is involved in epitope recognition, which is consistent with the V in the PGT121 and 10-1074 structures. L Third, the V identified as interacting with glycans may potentially explain the unusual tilt of CDRH3 toward H The majority of residues differ between PGT121 and 10-1074, which rationalizes the different binding profiles in glycan microarrays and potentially explains the different fine specificities revealed in protein binding experiments. Fourth, the exchange of crystallographically identified glycan contact residues between PGT121 and 10-1074 partially transferred their properties: PGT121GM Like 10-1074, it did not bind to protein-free glycans, but PGT121 GM and 10-1074 GM Both PGT121 and PGT122 conserved near-wild-type binding to purified YU-2 gp120 / gp140. GM retained the ability to neutralize some virus strains neutralized by wild-type PGT121 and 10-1074, but failed to neutralize strains that were PGT121-sensitive / 10-1074-resistant, providing evidence that the glycan-binding motif is essential for the neutralizing activity of PGT121 against 10-1074-resistant strains. Consistent with the transfer of crystallographically identified glycan motifs for reciprocal exchange, and consistent with the hypothesis that the epitopes of PGT121-like and 10-1074-like antibodies are related, 10-1074 GM The neutralizing potency of is increased or unaffected relative to 10-1074, and in some cases, GM potently neutralized PGT121-susceptible / 10-1074-resistant strains. In analyses of gp120 sequences from strains for which PGT121 neutralization data are available, other than interactions with PNGS at Asn332gp120 for viruses susceptible to PGT121-like and 10-1074-like antibodies, no clear patterns of PNGS utilization emerged for different categories of virus strains (PGT121-susceptible / 10-1074-susceptible, PGT121-susceptible / 10-1074-resistant, PGT121-resistant / 10-1074-susceptible), except that 10-1074-resistant strains generally lacked Asn332gp120-associated PNGS.

[0160] Example 9 Passive transfer of anti-HIV-1 neutralizing mAb in vivo Five isolated potent and broadly active anti-HIV neutralizing monoclonal antibodies were administered to rhesus macaques, who were challenged intrarectally 24 hours later with one of two different SHIVs. Combined results from 60 challenged animals demonstrated protective neutralizing titers in plasma of approximately 1:100 that prevented virus acquisition in 50% of exposed macaques.

[0161] Animal testing The macaques used in this study were of MHC class IMamu-A. * The results were negative for the 01 allele.

[0162] Construction of R5-tropic SHIVDH12-V3AD8 These V3 sequences were introduced into the genetic background of pSHIVDH12_CL7 molecular clone (J. of Virology 78, 5513-5519 (2004)) using PCR mutagenesis with primers corresponding to the 5' and 3' halves of the gp120 V3 coding region of SHIVAD8EO (PNAS 109, 19769-19774 (2012)) (forward primer: AGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCACATTGCAACATTAGTAAAGTAAAATGGC, and reverse primer: TCCTGGTCCTATATGTATACTTTTCCTTGTATTGTTGTTGGGTCTTGTACAATTAATTTCTACAGTTTCATTC) using Platinum PFX DNA polymerase (Invitrogen). After gel purification, the PCR product was treated with T4 polynucleotide kinase (GibcoBRL) and blunt-end ligated to generate pSHIVDH12_V3AD8, which was used to transform competent cells.

[0163] virus Virus stocks were prepared by transfecting 293T cells with SHIVAD8EO or SHIVDH12-V3AD8 molecular clones using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Culture supernatants were harvested after 48 h and aliquots were stored at -80°C until use. Concanavalin A-stimulated rhesus PBMCs (2 × 10 in 500 μL) were used for the transfection. 6Cells) were infected with transfected cell supernatants by spinoculation for 1 h (J. of Virology 74, 10074-10080 (2000)), mixed with an equal number / volume of activated PBMCs, and cultures were maintained for at least 12 days with daily replacement of culture medium. Samples of supernatant medium were pooled around the time of peak RT production to prepare individual virus stocks.

[0164] antibody Eleven monoclonal antibodies (VRC01, NIH45-46, 45-46G54W, 45-46m2, 3BNC117, 12A12, 1NC9, and 8ANC195, 10-1074, PGT121, and PGT126) were isolated and produced. DEN3, a dengue virus NS1-specific human IgG1 monoclonal antibody (PNAS 109, 18921-18925 (2012)), or control human IgG (NIH Nonhuman Primate Reagent Resource http: / / www.nhpreagents.org) were used in this study as negative control antibodies. The monoclonal antibodies selected for preexposure passive transfer were administered intravenously 24 hours before virus challenge infection.

[0165] Quantification of plasma viral RNA levels Viral RNA levels in plasma were determined by real-time reverse transcription-PCR (ABI Prism 7900HT sequence detection system; Applied Biosystems).

[0166] Plasma antibody concentration Concentrations of administered monoclonal antibodies in monkey plasma were measured by enzyme-linked immunosorbent assay (ELISA) using recombinant HIV-1 JRFL gp120 (Progenics Pharmaceuticals) or HIVIIIB (Advanced Biotechnology inc) (J. of Virology 75, 8340-8347 (2001)). Briefly, microtiter plates were coated with HIV-1 gp120 (2 μg / mL) and incubated overnight at 4°C. Plates were washed with PBS / 0.05% Tween-20 and blocked with 1% (vol / vol) BSA. After blocking, serial dilutions of antibody or plasma samples were added to the plates and incubated for 1 h at room temperature. Binding was detected with goat anti-human IgG F(ab)2 fragment conjugated to alkaline phosphatase (Pierce) and visualized with SIGMAFAST OPD (Sigma-Aldrich). The decay half-life of neutralizing monoclonal antibodies was calculated by a monoexponential decay equation based on plasma concentrations starting on the 5th or 7th day after antibody administration (J. of Virology 84, 1302-1313 (2010)).

[0167] Neutralization assay The in vitro potency and neutralizing activity of each mAb present in plasma samples collected from rhesus macaques was evaluated by two types of neutralization assays; 1) TZM-bl entry assay with pseudotyped challenge virus (AIDS Res Hum Retroviruses 26, 89-98 (2010)) or 2) 14-day PBMC replication assay with replication-competent virus (J. of Virology 76, 2123-2130 (2002)). For the TZM-bl assay, serially diluted mAbs or plasma samples were incubated with pseudotyped viruses expressing the env gene from SHIVAD8EO or SHIVDH12_V3AD8, which were prepared by cotransfecting 293T cells with pNLenv1 and pCMV vectors expressing the respective envelope proteins (J. of Virology 84, 4769-4781 (2010)). The 50% neutralizing dose (IC50) titer was calculated as the dilution that caused a 50% decrease in relative luminescence units (RLU) compared to the levels in virus control wells after subtraction of cell control RLU (J. of Virology 84, 1439-1452 (2010)). The neutralizing phenotype (titer level) of the SHIVDH12_V3AD8 molecular clone was determined by the TZM-bl cell assay using plasma samples from a cohort study that exhibits broad neutralizing activity against subtype B HIV-1 isolates (J. of General Virology 91, 2794-2803 (2010)).

[0168] Determination of animal protective titers and statistical analysis Calculations of plasma neutralizing titers for each R5 SHIV that would result in prevention of viral acquisition in 50 or 80% of virus-challenged infected animals were performed using the method of Reed and Muench (Am J Hyg 27, 493-497 (1938)). One significant outlier animal (DEW7) was omitted from the calculations. Probit regression was used to model the relationship between the plasma titers required to confer sterilizing immunity in vivo using all 60 passively immunized monkeys (Cambridge University Press, Cambridge, England, ed. 3rd, 2007), including p-values ​​from this model based on likelihood ratio tests. Plasma titers required for various in vivo protection levels (33%, 50%, 80%, 90% and 95%) were determined from the probit model estimates, and 90% confidence intervals were constructed using bootstrap methods.

[0169] result: SHIVDH12_V3AD8 had Tier 2 anti-HIV-1 neutralization susceptibility properties similar to SHIVAD8EO (Table 13). Rhesus macaques inoculated intravenously or intrarectally with SHIVDH12_V3AD8 exhibited peak viremia ranging from 105 to 107 viral RNA copies / mL of plasma at 2 to 3 weeks post-infection (PI). In most SHIVDH12_V3AD8-infected animals, plasma viral loads declined to background levels between 8 and 20 weeks PI.

[0170] The neutralization sensitivity of SHIVAD8EO to 11 recently reported broadly reactive anti-HIV-1 mAbs was initially determined in the TZM-bl assay system (Figures 11A and B). Eight of these antibodies, VRC01, NIH45-46(23), 45-46G54W, 45-46m2, 3BNC117, 12A12, 1NC9 and 8ANC195, targeted the gp120 CD4 bs (Science 333, 1633-1637 (2011)), and three, 10-1074, PGT121 and PGT126 (Nature 477, 466-470 (2011)), were dependent on the presence of the HIV-1 gp120 N332 glycan. When tested against SHIVAD8EO, all three glycan-dependent mAbs exhibited greater potency than the CD4 bs mAb (FIG. 11A). The IC50 values ​​for the three mAbs targeting gp120 N332 glycan ranged from 0.09 to 0.15 μg / mL. The CD4 bs mAbs exhibited a much broader range of IC50 neutralizing activity (0.14 to 6.34 μg / mL), with 3BNC117 being the most potent. A similar hierarchy of neutralizing mAb potency (glycan-dependent > CD4 bs-dependent) was observed for SHIVDH12-V3AD8, but its neutralizing activity was distributed over a much broader (>100-fold) range compared to the IC50 values ​​observed for SHIVAD8EO (FIG. 11B). SHIVDH12-V3AD8 was somewhat more sensitive to glycan-targeting mAbs and more resistant to CD4 bs neutralizing mAbs than SHIVAD8EO.

[0171] Based on the results shown in Figure 11, five neutralizing mAbs were selected for pre-exposure passive transfer studies: VRC01 (as this was the first CD4bs NAb of the newly isolated broadly acting NAbs to be characterized); CD4bs mAbs 45-46m2 and 3BNC117 (both of which exhibited strong neutralizing activity against SHIVAD8EO and SHIVDH12-V3AD8); and the gp120 N332 glycan-dependent mAbs, PGT121 and 10-1074.

[0172] The protocol for the passive transfer experiment was intravenous administration of decreasing doses of neutralizing mAbs and intrarectal challenge infection of the animals 24 hours later. The goal was to prevent viral acquisition, and we selected an SHIV challenge infection dose of sufficient size to establish in vivo infection after a single inoculation, with the knowledge that repeated administration of humanized anti-HIV mAbs to individual macaques may reduce their efficacy and / or possibly induce anaphylactic reactions. In this regard, we have previously performed intrarectal titration of SHIVAD8 in rhesus macaques and reported that inoculation of 1 x 103 TCID50 determined by end-point dilution in rhesus macaque PBMCs is equivalent to administering approximately 3 animal infectious doses50 (AID50) (J. of virology 86, 8516-8526 (2012)). Indeed, a single intrarectal inoculation of 3 AID50 resulted in successful establishment of infection with SHIVAD8EO and SHIVDH12-V3AD8 in 10 of 10 rhesus macaques.

[0173] As a control for the first passive transfer experiment, animals were administered an anti-dengue virus NS1 IgG1 mAb intravenously and challenged with SHIVAD8EO 24 hours later. Both monkeys (ML1 and MAA) became rapidly infected and developed peak levels of plasma viremia at 2 weeks PI. VRC01 was the first anti-HIV-1 neutralizing mAb tested for protection against viral acquisition and was administered to two macaques at a dose of 50 mg / kg. One of the two inoculated macaques (DEGF) was completely protected from SHIVAD8EO challenge infection with no evidence of plasma viremia or cell-associated viral DNA over the 45-week observation period. The other 50 mg / kg VRC01 recipient (DEH3) became infected but had a delayed peak plasma viremia until 5 weeks PI. Two additional macaques administered a lower dose of VRC01 (20 mg / kg) were not protected from SHIVAD8EO challenge infection. These results are summarized in Table 13.

[0174] The protective properties of PGT121 against SHIVAD8EO challenge infection were next investigated. PGT121 was one of the most potent glycan-targeting neutralizing mAbs measured in the TZM-bl assay (Figure 11). Based on the results obtained with VRC01, we chose to start with in vivo PGT121 mAb titration at 20 mg / kg. Two monkeys (KNX and MK4) that received challenge infection survived the SHIVAD8EO challenge infection. When lower doses of PGT121 were administered (i.e., 5 mg / kg, 1 mg / kg, or 0.2 mg / kg), 1 of 2 animals, 2 of 2 animals, and 0 of 2 animals were protected, respectively (Table 13).

[0175] The ability of VRC01 and PGT121 mAbs to prevent SHIVDH12-V3AD8 acquisition was similarly evaluated (Table 13). Results obtained with VRC01 were comparable to those observed with SHIVAD8EO challenge infection: one of two recipients at 30 mg / kg was protected from establishing SHIVDH12-V3AD8 infection. PGT121 mAb was significantly more effective than VRC01 in preventing SHIVDH12-V3AD8 acquisition: two of two recipients at 0.2 mg / kg PGT121 survived infection. PGT121 also appeared to be somewhat more effective in preventing SHIVDH12-V3AD8 against SHIVAD8EO in vivo infection (Table 13). This result is consistent with the eight-fold difference in IC50 values ​​of PGT121 for neutralization of the two SHIVs in in vitro assays (Figure 11).

[0176] The results of passive transfer of 10-1074, 3BNC117 or 45-46m2 neutralizing mAbs to rhesus macaques followed by challenge infection with either SHIVAD8EO or SHIVDH12-V3AD8 are summarized in Table 13. 10-1074 mAb potently blocked in vivo acquisition of both SHIVs. CD4bs 3BNC117 and 45-46m2 mAbs were selected for passive transfer to macaques based on their IC50 values ​​against both SHIVs in the in vitro neutralization experiments shown in Figure 11. 3BNC117 successfully blocked SHIVAD8EO infection in two of two monkeys at 5 mg / kg but not in the other two animals given a dose of 1 mg / kg (Table 13). This was similar to the results observed when the same amount of 3BNC117 was administered to macaques challenged with SHIVDH12-V3AD8 (1 of 2 infected at 5 mg / kg and 1 of 2 infected at 1 mg / kg).

[0177] Plasma samples taken from passively transferred macaques at various time points were analyzed by HIV-1 gp120 ELISA to determine neutralizing mAb concentrations. In general, the plasma concentration of each mAb at the time of challenge infection (24 hours after antibody administration) correlated with the dose of antibody administered (Table 13).

[0178] The relationship between plasma mAb concentration and in vivo protection is shown in Figure 12. Of the five neutralizing mAbs evaluated, PGT121 was clearly the most effective against both viruses, with SHIVDH12-V3AD8 exhibiting somewhat greater susceptibility to this mAb (two of two monkeys were protected at plasma concentrations of 0.2 μg / mL). In contrast, plasma concentrations of VRC01 of approximately 400 μg / mL were required to protect one of two animals against the same SHIVDH12-V3AD8 challenge virus (Table 13). The most potent CD4 bs mAb administered to macaques in this study, 3BNC117, was also approximately 6- to 10-fold more effective than VRC01 in preventing acquisition of either SHIV (Figure 12, Table 13).

[0179] The circulating half-lives of the PGT121, 10-1074, 3BNC117, and VRC01 mAbs were fairly similar: 3.5 days, 3.5 days, 3.3 days, and 3.1 days, respectively. In contrast, the half-life of 45-46m2 was extremely short and could not be determined. Based on plasma mAb concentrations in several macaques 24 hours after administration of 20 mg / kg of the humanized neutralizing mAb (i.e., approximately 250 μg / mL [Table 13]), two monkeys that received 20 mg / kg of 45-46m2 had plasma mAb concentrations of only 15.0 and 17.6 μg / mL, which was a greater than 95% decay compared to the other neutralizing mAbs at 24 hours.

[0180] When macaques were challenged with SHIVAD8EO and SHIVDH12-V3AD8, neutralization titers were measured using plasma samples collected 24 hours after mAb administration. As shown in Table 13, a good correlation was observed between antiviral plasma neutralization titers and protection from SHIV infection. Administration of the two glycan-dependent mAbs (PGT121 and 10-1074) resulted in clearly the highest titers of anti-HIV-1 neutralizing activity at the time of viral challenge. The titers measured in recipients of the 45-46m2 mAb were at or below the limit of detection because of its extremely short in vivo half-life.

[0181] Neutralizing titers, measured in plasma, required to prevent virus acquisition in 50% of challenged monkeys were calculated using the method described by Reed and Muench (Am J Hyg 27, 493-497 (1938)). These protective titers were derived separately for 28 monkeys challenged with SHIVAD8EO or 32 monkeys challenged with SHIVDH12-V3AD8 (Tables 15 and 16). Plasma neutralizing titers required to protect 50% of animals challenged with SHIVAD8EO or SHIVDH12-V3AD8 were calculated to be 1:115 and 1:96, respectively. Because these similar titers were obtained 1) after SHIV challenge infection by the same route and inoculum size and 2) after administration of the same panel of neutralizing mAbs, the neutralization data from all 60 animals were combined and subjected to probit regression to examine the relationship between plasma neutralizing titers and in vivo protection. As further confirmation, when a term for the SHIV virus was included in the probit regression model for all 60 macaques, there was no evidence of a difference between the two SHIV viruses (p=0.16). When applied to the entire group of 60 macaques, the probit regression estimated that a plasma neutralization titer of 1:104 would prevent virus acquisition in 50% of the animals. Probit analysis of the data also estimated that a 50% plasma neutralization titer of 1:57 or 1:329 would protect 33% or 80% of exposed animals, respectively.

[0182] Example 10. Administration of neutralizing mABs to an HIV in vivo model of chronic infection Summary of methods: The neutralizing activity of broadly active 3BNC11724 and 10-107423 neutralizing mAbs against SHIVAD8EO was initially determined in the TZM-bl cell system against SHIVAD8EO. Their ability to block viral acquisition or control plasma viremia in chronically infected animals challenged with R5-tropic SHIVAD8EO was assessed by monitoring plasma viral load and cell-associated viral nucleic acid; levels of CD4+ T cell subsets were measured by flow cytometry. SGA analysis of circulating viral variants and determination of antibody levels in plasma. Plasma concentrations of NAbs were determined by measuring neutralizing activity against HIV-1 pseudovirus preparations that were only sensitive to either 10-1074 or 3BNC117.

[0183] result: Two groups of chronically infected macaques were evaluated. The first group consisted of two clinically asymptomatic animals (DBZ3 and DC99A) that had been infected for 159 weeks and continued to show a similar and significant decline in circulating CD4+ T cells (Table 17). The treatment to treat ongoing SHIV infection was the combined administration of 101074 and 3BNC117 at a dose of 10 mg / kg. At the time of mAb administration, plasma viral loads in macaques DBZ3 and DC99A were 1.08×104 and 7.6×103 RNA copies / mL, respectively. Both monkeys responded to the combined anti-HIV-1 mAb treatment with immediate and rapid reduction in plasma viremia to undetectable levels within 7 to 10 days. Suppression of measurable SHIVAD8EO in the plasma of macaques DBZ3 and DC99A after a single dose of the two mAbs lasted for 27 and 41 days, respectively. In each case, plasma viremia reverted to pretreatment levels.

[0184] A second group of three animals (DBX3, DCF1 and DCM8), each of which had also been infected with SHIVAD8EO for more than 3 years and had clinical symptoms of intermittent diarrhea and / or anorexia, were treated with the two neutralizing antibodies (Table 17). At the time of mAb administration, the level of circulating CD4+ T cells in macaque DCM8 was only 43 cells / μL and was somewhat higher in animals DCF1 (105 cells / μL) and DBXE (158 cells / μL). Plasma viral load was over 105 RNA copies / mL in animals DBXE and DCF1 and was significantly lower in monkey DCM8 (1.59×103 RNA copies / mL). Administration of the two mAbs to monkey DBXE resulted in a biphasic decline from 2.0×105 RNA copies on day 0 to undetectable plasma levels on day 20. This was followed within a few days by a return of high circulating viral levels in DBXE. Macaque DCM8, which has a lower plasma viral load and very low numbers of circulating CD4+ T cells, experienced a rapid decline in viremia to undetectable levels between days 6 and 20 after initiation of mAb treatment. Finally, DCF1, an animal previously reported to produce broadly reactive anti-HIV-1 NAbs, exhibited a transient and relatively modest 27-fold decline in plasma viremia by day 6 in response to combination mAb therapy, after which viral load returned to high pretreatment levels.

[0185] PBMC-associated viral RNA and DNA levels were also determined before and after antibody administration (Table 18). For each animal, mAb treatment resulted in a reduction in cell-associated viral RNA levels that correlated well with plasma viral load measurements. No consistent pattern was observed for cell-associated viral DNA levels as a result of antibody treatment. Administration of neutralizing mAbs to chronically SHIVAD8EO-infected monkeys also had a beneficial effect on circulating CD4+ T cell levels, especially in animals with very high viral loads. CD4+ T cell numbers in macaques DBXE and DCF1 increased 2- to 3-fold during the period of mAb-mediated viral suppression but gradually declined to pretreatment levels as viremia became detectable again.

[0186] Plasma concentrations of each mAb were determined by measuring plasma neutralizing activity against selected HIV-1 pseudovirus strains sensitive to one or the other but not both antibodies (Figure 13A). Suppression of SHIVAD8EO viremia was maintained in all treated animals until a threshold plasma mAb concentration of approximately 1 to 3 μg / mL was reached (Figures 13B and 13C). This was also true for macaque DCF1, where a modest, transient decrease in plasma viral RNA levels was observed. Interestingly, mAbs administered to clinically symptomatic macaques DCM8 and DCF1 had shortened or undetectable half-lives. As mentioned before, macaque DCM8 had extremely low CD4+ T cell levels (43 cells / μL plasma) and macaque DCF1 had to be euthanized on day 56 after initiation of treatment due to its deteriorating clinical condition. Autopsy of DCF1 revealed severe enteropathy characterized by disseminated gastrointestinal cryptosporidiosis, pancreatitis and cholangitis.

[0187] SGA analysis was used to determine whether amino acid substitutions had occurred in the gp120 regions previously shown to affect susceptibility to 10-1074 or 3BNC117 mAbs. In each case, rebound virus present in plasma after immunotherapy was unchanged. To further test the susceptibility of re-emerging virus, a combination therapy of 10-1074 and 3BNC117 (10 mg / kg of each) was administered again to two clinically asymptomatic monkeys (DBZ3 and DC99A). The viral load of each animal again dropped rapidly and became undetectable at day 7 of the second immunotherapy cycle. Viremia was suppressed for 7 days in macaque DBZ3 and for more than 21 days in monkey DC99A. Taken together, these results suggest that the re-emergence of virus after the first treatment cycle in these two animals represented insufficient mAb levels in vivo rather than antibody-selected viral resistance.

[0188] [Table 3]

[0189]

Table 4

[0190]

Table 5

[0191]

Table 6

[0192]

Table 7

[0193]

Table 8

[0194]

Table 9

[0195]

Table 10

[0196]

Table 11

[0197]

Table 12

[0198]

Table 13

[0199]

Table 14

[0200] [Table 15]

[0201] [Table 16]

[0202] [Table 17]

[0203] [Table 18]

[0204] The above examples and descriptions of the preferred embodiments should be considered as illustrative, rather than limiting, of the present invention as defined by the claims. As will be readily understood, numerous variations and combinations of the above features can be used without departing from the invention as set forth in the claims. Such variations are not considered as a departure from the scope of the present invention, and all such variations are to be construed as falling within the scope of the following claims. All references cited herein are hereby incorporated by reference in their entirety.

Claims

1. (i)(1) a complementarity determining region (CDR) H1 comprising the amino acid sequence of SEQ ID NO:69, a CDRH2 comprising the amino acid sequence of SEQ ID NO:70, a CDRH3 comprising the amino acid sequence of SEQ ID NO:71, a CDRL1 comprising the amino acid sequence of SEQ ID NO:72, a CDRL2 comprising the amino acid sequence of SEQ ID NO:73, and a CDRL3 comprising the amino acid sequence of SEQ ID NO:74; (2) a CDRH1 comprising the amino acid sequence of SEQ ID NO:39, a CDRH2 comprising the amino acid sequence of SEQ ID NO:40, a CDRH3 comprising the amino acid sequence of SEQ ID NO:41, a CDRL1 comprising the amino acid sequence of SEQ ID NO:42, a CDRL2 comprising the amino acid sequence of SEQ ID NO:43, and a CDRL3 comprising the amino acid sequence of SEQ ID NO:44; (3) a CDRH1 comprising the amino acid sequence of SEQ ID NO:51, a CDRH2 comprising the amino acid sequence of SEQ ID NO:52, a CDRH3 comprising the amino acid sequence of SEQ ID NO:53, a CDRL1 comprising the amino acid sequence of SEQ ID NO:54, a CDRL2 comprising the amino acid sequence of SEQ ID NO:55, and a CDRL3 comprising the amino acid sequence of SEQ ID NO:56; (4) CDRH1 comprising the amino acid sequence of SEQ ID NO:57, CDRH2 comprising the amino acid sequence of SEQ ID NO:58, CDRH3 comprising the amino acid sequence of SEQ ID NO:59, CDRL1 comprising the amino acid sequence of SEQ ID NO:60, CDRL2 comprising the amino acid sequence of SEQ ID NO:61, and CDRL3 comprising the amino acid sequence of SEQ ID NO:62; (5) CDRH1 comprising the amino acid sequence of SEQ ID NO:63, CDRH2 comprising the amino acid sequence of SEQ ID NO:64, CDRH3 comprising the amino acid sequence of SEQ ID NO:65, CDRL1 comprising the amino acid sequence of SEQ ID NO:66, CDRL2 comprising the amino acid sequence of SEQ ID NO:67, and CDRL3 comprising the amino acid sequence of SEQ ID NO:68; (6) CDRH1 comprising the amino acid sequence of SEQ ID NO:75, CDRH2 comprising the amino acid sequence of SEQ ID NO:76, CDRH3 comprising the amino acid sequence of SEQ ID NO:77, CDRL1 comprising the amino acid sequence of SEQ ID NO:78, CDRL2 comprising the amino acid sequence of SEQ ID NO:79, and CDRL3 comprising the amino acid sequence of SEQ ID NO:80; (7) CDRH1 comprising the amino acid sequence of SEQ ID NO: 81, CDRH2 comprising the amino acid sequence of SEQ ID NO: 82, CDRH3 comprising the amino acid sequence of SEQ ID NO: 83, and CDRH4 comprising the amino acid sequence of SEQ ID NO:

84. CDRL1 comprising the amino acid sequence of SEQ ID NO:85, CDRL2 comprising the amino acid sequence of SEQ ID NO:86; (8) CDRH1 comprising the amino acid sequence of SEQ ID NO: 87, CDRH2 comprising the amino acid sequence of SEQ ID NO: 88, CDRH3 comprising the amino acid sequence of SEQ ID NO: 89, CDRL1 comprising the amino acid sequence of SEQ ID NO: 90, CDRL2 comprising the amino acid sequence of SEQ ID NO: 91, and CDRL3 comprising the amino acid sequence of SEQ ID NO: 92; (9) CDRH1 comprising the amino acid sequence of SEQ ID NO:93, CDRH2 comprising the amino acid sequence of SEQ ID NO:94, CDRH3 comprising the amino acid sequence of SEQ ID NO:95, CDRL1 comprising the amino acid sequence of SEQ ID NO:96, CDRL2 comprising the amino acid sequence of SEQ ID NO:97, and CDRL3 comprising the amino acid sequence of SEQ ID NO:98; (10) CDRH1 comprising the amino acid sequence of SEQ ID NO:99, CDRH2 comprising the amino acid sequence of SEQ ID NO:100, CDRH3 comprising the amino acid sequence of SEQ ID NO:101, CDRL1 comprising the amino acid sequence of SEQ ID NO:102, CDRL2 comprising the amino acid sequence of SEQ ID NO:103, and CDRL3 comprising the amino acid sequence of SEQ ID NO:104; or (11) CDRH1 comprising the amino acid sequence of SEQ ID NO: 131, CDRH2 comprising the amino acid sequence of SEQ ID NO: 132, CDRH3 comprising the amino acid sequence of SEQ ID NO: 133, CDRL1 comprising the amino acid sequence of SEQ ID NO: 134, CDRL2 comprising the amino acid sequence of SEQ ID NO: 135, and CDRL3 comprising the amino acid sequence of SEQ ID NO: 136; and (ii) a pharma- ceutically acceptable carrier.

2. The pharmaceutical composition described in claim 1, wherein the anti-HIV antibody or its antigen-binding portion comprises a heavy chain variable region comprising any one of the amino acid sequences of SEQ ID NOs: 3, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 129.

3. The pharmaceutical composition of claim 1, wherein the anti-HIV antibody or antigen-binding portion thereof comprises a light chain variable region comprising any one of the amino acid sequences of SEQ ID NOs: 4, 8, 10, 12, 14, 16, 18, 20, 22, 24 and 130.

4. The pharmaceutical composition of claim 1, wherein the anti-HIV antibody or antigen-binding portion thereof comprises a heavy chain variable region and a light chain variable region comprising each of the amino acid sequences of SEQ ID NOs:13-14, 3-4, 7-8, 9-10, 11-12, 15-16, 17-18, 19-20, 21-22, 23-24, and 129-130.

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