Terrestrial shellfish attractant composition

Abstract

This composition for attracting terrestrial mollusks contains at least one selected from the group consisting of fatty acids having 14-30 carbons in the fatty acid portion, fatty acid esters having 14-30 carbons in the fatty acid portion, and fatty acid amides having 14-30 carbons in the fatty acid portion.

Description

[Technical Field]

[0001] The present invention relates to a terrestrial snail attractant composition. [Background technology]

[0002] Land snails and slugs are causing severe damage to crops such as citrus fruits and leafy vegetables.

[0003] Common methods for controlling these land snails include spraying attractant insecticides containing metaldehyde or ferric phosphate, but the bait used as the attractant is not very effective, and there is room for improvement in the rate of shellfish deaths and the rate of climbing inhibition.

[0004] Other proposed slug repellents include a water-insoluble slug repellent containing banana fruit (Patent Document 1), a slug repellent containing essential ingredients such as edible slug powders (e.g., plant or animal powders), metaldehyde, a glue, and a pH adjuster (Patent Document 2), a gastropod bait containing an attractant bait component and a gastropod attractant / control compound with a viscosity of 100,000 to 2,000,000 cP (Patent Document 3), a slug / snail repellent containing metaldehyde and sake lees (Patent Document 4), and a granular attractant / repellent containing a pesticide active ingredient and sake lees (Patent Document 5). However, the attractants used in these repellents lack the specific attractant effect on terrestrial snails, and because they contain large amounts of protein, starch, sugars, etc., they have problems such as low long-term stability in external environments and rapid deterioration of their attractant effect. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Japanese Patent Application Publication No. 02-28103 [Patent Document 2] Japanese Patent Application Publication No. 08-175906 [Patent Document 3] Japanese Patent Application Publication No. 09-110603 [Patent Document 4] Japanese Patent Application Publication No. 10-25207 [Patent Document 5] Japanese Patent Application Laid-Open No. 2001-206810 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present invention is to provide a new composition for attracting land snails that has an attractive effect on land snails. [Means for solving the problem]

[0007] In order to solve the above problems, the inventors conducted extensive research and found that an attractive effect on terrestrial snails can be achieved by using at least one attractant selected from the group consisting of fatty acids having 14 to 30 carbon atoms in the fatty acid moiety, fatty acid esters having 14 to 30 carbon atoms in the fatty acid moiety, and fatty acid amides having 14 to 30 carbon atoms in the fatty acid moiety.

[0008] The present invention has been completed based on these findings and includes the following broad aspects. [Section 1] A composition for attracting terrestrial snails, comprising at least one member selected from the group consisting of fatty acids having 14 to 30 carbon atoms in the fatty acid moiety, fatty acid esters having 14 to 30 carbon atoms in the fatty acid moiety, and fatty acid amides having 14 to 30 carbon atoms in the fatty acid moiety. [Section 2] Item 1. A composition for attracting land snails according to Item 1, further comprising an insecticidal component effective against land snails. [Section 3] Item 3. The terrestrial snail attractant composition according to Item 1 or 2, comprising at least one selected from the group consisting of palmitic acid, linoleic acid, oleic acid, stearic acid, methyl palmitate, methyl linoleate, methyl oleate, methyl stearate, ethyl palmitate, ethyl linoleate, ethyl oleate, ethyl stearate, palmitic acid amide, linoleic acid amide, oleic acid amide, stearic acid amide, erucic acid amide, palmitoylethanolamide, and oleylethanolamide. [Section 4] Item 4. The composition for attracting terrestrial snails according to any one of Items 1 to 3, wherein the terrestrial snails are Styelidromata. [Section 5] Item 5. A composition for attracting terrestrial snails according to any one of Items 1 to 4, wherein the terrestrial snails are of the family Hemicentrotidae. [Section 6] Item 6. The composition for attracting terrestrial snails according to any one of Items 1 to 5, wherein the terrestrial snails are Hemicentrotus maximus or Hemicentrotus maximus. [Section 7] Item 7. A land snail trap comprising the land snail attracting composition according to any one of Items 1 to 6. [Section 8] Item 7. A method for attracting terrestrial snails, comprising the step of placing the terrestrial snail attracting composition according to any one of Items 1 to 6 or the terrestrial snail trap according to Item 7. [Section 9] Item 9. A method for attracting terrestrial snails according to Item 8, wherein the plants are placed on a predicted route that the terrestrial snails will take to come into contact with the plant, or inside or around the field where the plant is cultivated. [Effects of the Invention]

[0009] According to the present invention, a new composition for attracting terrestrial snails that exhibits an attractive effect on terrestrial snails can be provided. DETAILED DESCRIPTION OF THE INVENTION

[0010] In one aspect, the present invention provides a composition for attracting terrestrial snails, which comprises at least one selected from the group consisting of fatty acids having a fatty acid moiety of 14 to 30 carbon atoms, fatty acid esters having a fatty acid moiety of 14 to 30 carbon atoms, and fatty acid amides having a fatty acid moiety of 14 to 30 carbon atoms.

[0011] The terrestrial snails in the present invention are preferably Stylommatidae. Examples of Stylommatidae include snails of the family Achatidae, such as the giant snail, the amber-headed snail, and the pale-headed snail; the family Achatidae, such as the giant African snail; the family Achatidae, such as the giant African snail; the family Achatidae, such as the giant mussel; the family Achatidae, such as the apple snail; slugs of the family Achatidae, such as the red slug, the brown slug, and the field slug; the family Achatidae, such as the slug and the mountain slug; and the family Achatidae, such as the garden slug. Among these, snails and snails of the family Achatidae are preferred, and Achatidae or the amber-headed snail are more preferred.

[0012] In the present invention, fatty acids having 14 to 30 carbon atoms in the fatty acid moiety, fatty acid esters having 14 to 30 carbon atoms in the fatty acid moiety, and fatty acid amides having 14 to 30 carbon atoms in the fatty acid moiety are volatile attractant compounds for terrestrial snails.

[0013] The fatty acid having 14 to 30 carbon atoms in the fatty acid moiety is preferably a saturated or unsaturated fatty acid having 14 to 30 carbon atoms in the fatty acid moiety.

[0014] The number of carbon atoms in the fatty acid moiety of the fatty acid is 14 to 30, preferably 14 to 28, more preferably 14 to 26, even more preferably 14 to 24, particularly preferably 16 to 22, and most preferably 16 to 20.

[0015] The fatty acid having 14 to 30 carbon atoms in the fatty acid moiety can be any of the compounds described above and is not particularly limited. Examples include myristic acid, pentadecylic acid, palmitic acid, palmitoleic acid, margaric acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid, erucic acid, lignoceric acid, nervonic acid, and cerotic acid.

[0016] Among these, the fatty acids having 14 to 30 carbon atoms in the fatty acid moiety are preferably palmitic acid, linoleic acid, oleic acid, and stearic acid.

[0017] The fatty acid ester having 14 to 30 carbon atoms in the fatty acid moiety is preferably a saturated or unsaturated fatty acid ester having 14 to 30 carbon atoms in the fatty acid moiety.

[0018] The number of carbon atoms in the fatty acid moiety of the fatty acid ester is 14 to 30, preferably 14 to 28, more preferably 14 to 26, even more preferably 14 to 24, particularly preferably 16 to 22, and most preferably 16 to 20.

[0019] The fatty acid ester is preferably one obtained by dehydration condensation of an alcohol having 1 to 10 carbon atoms with a fatty acid having 14 to 30 carbon atoms. The number of carbon atoms in the alcohol-derived portion of the fatty acid ester is preferably 1 to 10, more preferably 1 to 6, even more preferably 1 to 4, particularly preferably 1 to 3, and most preferably 1 or 2. Examples of the fatty acid ester include methyl ester, ethyl ester, propyl ester, isopropyl ester, n-butyl ester, sec-butyl ester, tert-butyl ester, pentyl ester, hexyl ester, heptyl ester, and decyl ester, with methyl ester, ethyl ester, and propyl ester being preferred, and methyl ester being more preferred.

[0020] The fatty acid ester having 14 to 30 carbon atoms in the fatty acid moiety can be any of the compounds described above, and examples thereof include methyl esters of methyl myristate, methyl pentadecylate, methyl palmitate, methyl palmitoleate, methyl margarate, methyl stearate, methyl oleate, methyl linoleate, methyl linolenate, methyl arachidate, methyl arachidonate, methyl behenate, methyl erucate, methyl lignocerate, methyl nervate, and methyl cerotate; ethyl myristate, ethyl pentadecylate, methyl palmitate, methyl margarate, methyl stearate, methyl oleate, methyl linoleate, methyl arachidate, methyl arachidonate, methyl behenate, methyl erucate, methyl lignocerate, methyl nervate, and methyl cerotate; Ethyl esters of ethyl myristate, ethyl palmitoleate, ethyl margarate, ethyl stearate, ethyl oleate, ethyl linoleate, ethyl linolenate, ethyl arachidate, ethyl arachidonate, ethyl behenate, ethyl erucate, ethyl lignocerate, ethyl nervate, ethyl cerotate, etc.; propyl myristate, propyl pentadecylate, propyl palmitate, propyl palmitoleate, propyl margarate, propyl stearate, propyl oleate, propyl linoleate, propyl linolenate, arachidonic acid, ethyl arachidonic acid, ethyl arachidonic acid, ethyl erucate, ethyl lignocerate, ethyl nervate, ethyl cerotate, etc. Propyl esters such as propyl arachidonate, propyl behenate, propyl erucate, propyl lignocerate, propyl nervonate, and propyl cerotate; butyl esters such as butyl myristate, butyl pentadecylate, butyl palmitate, butyl palmitoleate, butyl margarate, butyl stearate, butyl oleate, butyl linoleate, butyl linolenate, butyl arachidate, butyl arachidonate, butyl behenate, and butyl erucate; pentyl myristate, pentyl pentadecylate, palmitate pentyl esters such as pentyl phosphate, pentyl palmitoleate, pentyl margarate, pentyl stearate, pentyl oleate, pentyl linoleate, pentyl linolenate, pentyl arachidate, and pentyl arachidonate; and hexyl esters such as hexyl myristate, hexyl pentadecylate, hexyl palmitate, hexyl palmitoleate, hexyl margarate, hexyl stearate, hexyl oleate, hexyl linoleate, hexyl linolenate, hexyl arachidate, and hexyl arachidonate.

[0021] Among these, fatty acid esters having 14 to 30 carbon atoms in the fatty acid moiety include methyl esters such as methyl myristate, methyl pentadecylate, methyl palmitate, methyl palmitoleate, methyl margarate, methyl stearate, methyl oleate, methyl linoleate, methyl linolenate, methyl arachidate, methyl arachidonate, methyl behenate, methyl lignocerate, methyl nervate, methyl cerotate, ethyl myristate, ethyl pentadecylate, and ethyl palmitate. Ethyl esters such as ethyl palmitoleate, ethyl margarate, ethyl stearate, ethyl oleate, ethyl linoleate, ethyl linolenate, ethyl arachidate, ethyl arachidonate, ethyl behenate, ethyl erucate, ethyl lignocerate, ethyl nervate, and ethyl cerotate are preferred, with methyl palmitate, methyl stearate, methyl oleate, methyl linoleate, ethyl palmitate, ethyl stearate, ethyl oleate, and ethyl linoleate being more preferred.

[0022] The fatty acid amide having a fatty acid moiety with 14 to 30 carbon atoms is preferably a saturated or unsaturated fatty acid amide having a fatty acid moiety with 14 to 30 carbon atoms. The fatty acid amide is preferably a primary fatty acid amide or an N-acylethanolamine.

[0023] The number of carbon atoms in the fatty acid moiety of the fatty acid amide is 14 to 30, preferably 14 to 28, more preferably 14 to 26, even more preferably 14 to 24, particularly preferably 16 to 22, and most preferably 16 to 20.

[0024] The fatty acid amide having 14 to 30 carbon atoms in the fatty acid moiety can be any of the compounds described above and can be used without any particular limitation. Examples of the fatty acid amide include primary fatty acid amides such as myristic acid amide, pentadecylic acid amide, palmitic acid amide, palmitoleic acid amide, margaric acid amide, stearic acid amide, oleic acid amide, linoleic acid amide, linolenic acid amide, arachidic acid amide, arachidonic acid amide, behenic acid amide, erucic acid amide (erucamide), lignoceric acid amide, nervonic acid amide, and cerotic acid amide; myristoylethanolamide; pentadecylic acid amide; palmitic acid amide; palmitoleic acid amide; margaric acid amide; stearic acid amide; oleic acid amide; linoleic acid amide; linolenic acid amide; arachidic acid amide; arachidonic acid amide; behenic acid amide; erucic acid amide (erucamide); lignoceric acid amide; nervonic acid amide; and cerotic acid amide. Examples of N-acylethanolamines include ethanolamides such as octanoylethanolamide, palmitoylethanolamide, palmitoleoylethanolamide, margalloylethanolamide, stearoylethanolamide, oleylethanolamide, linoleylethanolamide, linolenoylethanolamide, arachidoylethanolamide, arachidonoylethanolamide, behenoylethanolamide, erucoylethanolamide, lignoceroylethanolamide, nervonoylethanolamide, and cerotinoylethanolamide.

[0025] Among these, the number of carbon atoms in the fatty acid moiety is 14 The fatty acid amide having a molecular weight of 30 or less is preferably palmitic acid amide, stearic acid amide, oleic acid amide, linoleic acid amide, erucic acid amide, palmitoylethanolamide, or oleylethanolamide.

[0026] The fatty acids having 14 to 30 carbon atoms in the fatty acid moiety, the fatty acid esters having 14 to 30 carbon atoms in the fatty acid moiety, and the fatty acid amides having 14 to 30 carbon atoms in the fatty acid moiety may each be used alone or in combination of two or more.

[0027] The content of the attractant compound can be appropriately set depending on the formulation and attractant activity of the terrestrial snail attractant composition, but for example, when the compound is dissolved in a solvent to form a liquid formulation, the content is preferably 0.0001 to 1 part by mass, and more preferably 0.001 to 0.1 part by mass, per 100 parts by mass of the terrestrial snail attractant composition. By keeping the content within the above range, the attractant effect on terrestrial snails will be good.

[0028] The terrestrial snail attractant composition of the present invention preferably further contains an insecticidal component effective against terrestrial snails in addition to at least one selected from the group consisting of fatty acids having a fatty acid moiety with 14 to 30 carbon atoms, fatty acid esters having a fatty acid moiety with 14 to 30 carbon atoms, and fatty acid amides having a fatty acid moiety with 14 to 30 carbon atoms. By including an insecticidal component effective against terrestrial snails, it becomes possible to kill terrestrial snails attracted to the terrestrial snail attractant composition of the present invention.

[0029] The insecticidal component effective against terrestrial snails is not particularly limited as long as it has an insecticidal effect on terrestrial snails, and examples thereof include metaldehyde, ferric phosphate, azadirachtin, buprofezin, quinomethionate, chlorfenapyr, cyromazine, diafenthiuron, dicofol, dienochlor, endosulfan, flometoquin, flonicamid, flufenerim, flupyradifurone, hydramethylnon, hydroprene, indoxacarb, metaflumizone, methoprene, methoxychlor, Examples include pymetrozine, pyridalyl, pyrifluquinazone, pyriproxyfen, rotenone, spirotetramat, sulfoxaflor, tolfenpyrad, triflumezopyrim, tetraniliprole, broflanilide, acinonapyr, fluxamitamide, flupirim, triflumezopyrim, sodium oleate, diatomaceous earth, fatty acid glycerides, starch, rapeseed oil, adhesive (polybutene), propylene glycol mono-fatty acid ester, machine oil, nicotine sulfate, etc. These may be used alone or in combination of two or more.

[0030] The content of the insecticidal component effective against terrestrial snails is preferably 0.1 to 30 parts by mass, more preferably 0.5 to 10 parts by mass, per 100 parts by mass of the total amount of the terrestrial snail attractant composition.

[0031] The land snail attractant compositions of the present invention may contain additional ingredients such as solvents, surfactants, excipients, edible ingredients, preservatives, antioxidants, and / or anti-ingestion agents.

[0032] Examples of the solvent include alcohols such as methanol, ethanol, isopropanol, butanol, hexanol, cyclohexanol, phenoxyethanol, and benzyl alcohol; higher alcohols such as octyl alcohol, lauryl alcohol, and 3-methoxy-3-methyl-1-butanol; polyhydric alcohols such as ethylene glycol, propylene glycol, diethylene glycol, hexylene glycol, polyethylene glycol, and polypropylene glycol; glycol ethers such as methyl diglycol, methyl triglycol, ethylene glycol monobenzyl ether, ethylene glycol dimethyl ether, diethylene glycol monoethyl ether, propylene glycol monomethyl ether, and diethylene glycol monobenzyl ether; aromatic or aliphatic hydrocarbons such as xylene, toluene, phenylxylylethane, kerosene, light oil, hexane, cyclohexane, 1,2-dimethyl-4-ethylbenzene, methylnaphthalene, 1-phenyl-1-xylylethane, and 1-xylyl-1-(3-α-methylbenzylphenyl)ethane; diisobutyl adipate, dioleyl adipate, and the like. polybasic acid alcohol esters such as sorbitan monolaurate, sorbitan monooleate, and the like; polyhydric alcohol fatty acid esters such as sorbitan monolaurate, sorbitan monooleate, and the like; paraffinic hydrocarbons such as normal paraffin, isoparaffin, and liquid paraffin; ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone, and cyclohexanone; halogenated hydrocarbons such as chlorobenzene, dichloromethane, dichloroethane, and trichloroethane; Examples of suitable solvents include nitriles such as acetonitrile and isobutyronitrile; sulfoxides such as dimethyl sulfoxide; heterocyclic solvents such as sulfolane, γ-butyrolactone, N-methyl-2-pyrrolidone, N-ethyl-2-pyrrolidone, N-octyl-2-pyrrolidone, and 1,3-dimethyl-2-imidazolidinone; acid amides such as N,N-dimethylformamide and N,N-dimethylacetamide; acid alkylidenes such as propylene carbonate; vegetable oils such as soybean oil, coconut oil, rapeseed oil, tung oil, castor oil, and cottonseed oil; and vegetable essential oils such as orange oil, hyssop oil, and lemon oil.These may be used alone or in combination of two or more.

[0033] The surfactant may be any of nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants, without any particular limitation. Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene alkylaryl ethers, polyoxyethylene styrylphenyl ethers, polyoxyethylene alkyl esters, polyoxyethylene sorbitan alkylates, polyoxyethylene alkylene arylphenyl ethers, polyoxyethylene alkylene glycols, polyoxyethylene hydrogenated castor oil, polyoxyethylene alkylamines, polyoxyethylene fatty acid amides, and polyoxyethylene-type silicone surfactants; anionic surfactants such as polyoxyethylene alkyl ether phosphates, lignin sulfonates, alkylaryl sulfonates, polyoxyethylene alkylaryl ether sulfates, alkylnaphthalene sulfonates, dialkyl sulfosuccinates, polyoxyethylene styrylphenyl ether sulfates, alkylbenzene sulfonates, alkyl sulfonates, alkyl sulfates, and sodium salts of alkyl maleic acid co-condensates; amphoteric surfactants such as alkyl betaines and alkylamidopropyl betaines; and cationic surfactants such as alkylamine salts and quaternary ammonium salts. These may be used alone or in combination of two or more.

[0034] Examples of excipients include powders such as diatomaceous earth, silica, silica gel, calcium silicate, clay, dextrin, starch, carboxymethyl cellulose, methyl cellulose, wheat flour, etc. These may be used alone or in combination of two or more.

[0035] Examples of edible ingredients include cereal flours such as potato, bran, rice bran, and wheat flour, carbohydrates such as sugar, glucose, and starch syrup, animal powders such as pupa powder, krill, fish meal, and beef or pork powder, yeast, etc. These may be used alone or in combination of two or more.

[0036] Examples of preservatives include boric acid, sodium dehydroacetate, benzoic acid, sorbic acid and its salts, parahydroxybenzoic acid esters, etc. These may be used alone or in combination of two or more.

[0037] Examples of antioxidants include erythorbic acid, sodium erythorbate, dibutylhydroxytoluene, dl-α-tocopherol, nordihydroguaiaretic acid, methylhydroxyanisole, propyl gallate, guaiac butter, L-cysteine ​​hydrochloride, etc. These may be used alone or in combination of two or more.

[0038] Examples of the accidental ingestion prevention agent include chili pepper powder, amaranth, amaranth aluminum lake, erythrosine, erythrosine aluminum lake, new coccine, phloxine, rose bengal, acid red, tartrazine, tartrazine aluminum lake, sunset yellow FCF, sunset yellow FCF aluminum lake, fast green FCF, fast green FCF aluminum lake, brilliant blue FCF, brilliant blue FCF aluminum lake, indigo carmine, indigo carmine aluminum lake, β-carotene, copper chlorophyll, etc. These may be used alone or in combination of two or more.

[0039] In one embodiment, the present invention provides an attractant, attractant insecticide, attractant repellent, or repellent for terrestrial snails, comprising the terrestrial snail attractant composition.

[0040] These agents may be liquid or solid, but are preferably solid in order to provide sustained release and long-term action. Examples of solid dosage forms include pellets, powders, beads, and disks. Filter paper, absorbent cotton, sponges, and other porous substrates onto which the terrestrial snail attractant composition of the present invention has been applied or immersed can also be used as the agent.

[0041] In another embodiment, the present invention provides a terrestrial snail trap containing the terrestrial snail attractant composition. The trap is not particularly limited as long as it contains the terrestrial snail attractant composition of the present invention in a container. For example, the trap may have a trap structure that allows terrestrial snails to enter but not escape, or may have an adhesive mechanism such as an adhesive sheet provided inside the trap.

[0042] In another aspect, the present invention provides a method for attracting terrestrial snails, a method for preventing feeding damage by terrestrial snails, or a method for controlling terrestrial snails in plant cultivation. These methods use the terrestrial snail attracting composition of the present invention. In another aspect, the present invention provides a method for killing or exterminating terrestrial snails. These methods use the terrestrial snail attracting composition of the present invention, which contains an insecticidal component effective against terrestrial snails.

[0043] More specifically, these methods involve placing the terrestrial snail attractant composition, the agent, or a terrestrial snail trap of the present invention in a location where terrestrial snails are present or suspected to be present, such as a field, rice paddy, irrigation channel, ridge, orchard, garden, flowerpot, or around a house. The placement location is preferably on a predicted route that terrestrial snails will take to contact a plant (especially a plant where contact by terrestrial snails should be suppressed or eliminated), or inside or around the field where the plant is cultivated. As used herein, the term "periphery" refers to any area sufficient to suppress or eliminate contact by terrestrial snails, such as within 20 m of the periphery of the field. Furthermore, as used herein, the term "predicted route" broadly encompasses any predicted route from a location where terrestrial snails are present, a location where terrestrial snails are suspected to be present, or a location where traces of terrestrial snail presence have been confirmed, to the location of the plant or the location where the plant is cultivated.

[0044] Plants to which the present invention can be applied are not particularly limited as long as they are susceptible to feeding damage by terrestrial snails, and examples thereof include food crops such as wheat, rice, corn, potatoes, and beans; horticultural crops including vegetables, fruit trees, ornamental plants, and other useful trees; green manure crops; industrial crops; and fodder crops. Examples of horticultural crops include citrus fruits, peaches, figs, grapes, Japanese mustard spinach, cucumbers, tomatoes, lettuce, cabbage, pumpkins, watermelons, and melons. By applying the present invention to these plants, feeding damage by terrestrial snails can be prevented, and high yields can be achieved.

[0045] The terrestrial snail attracting composition of the present invention uses a highly stable, volatile attractant compound, and is therefore expected to be stable for a long period of time in external environments. The terrestrial snail trap and terrestrial snail attracting method using the terrestrial snail attracting composition of the present invention can stably attract terrestrial snails with a high attracting effect. [Example]

[0046] The present invention will be explained in more detail using Production Examples and Examples, but the present invention is not limited to these Examples.

[0047] <Example 1: Identification of compounds with attractant activity against giant snails> (1) Hexane extraction of secretory substances Two freeze-dried giant snails (total 0.64 g) were rinsed with 1 ml of hexane, and the hexane extract was collected.

[0048] (2) Bioassay of attractant activity of hexane extract solution An assay to detect whether snails are attracted to the volatile gases of the hexane extract solution was carried out using the following behavioral experiment.

[0049] A 150 mm diameter glass petri dish was placed horizontally, with 10 mm square filter paper placed at each end of the dish. 5 μl of hexane extract solution was then dropped onto one filter paper, and an equal amount of hexane alone was dropped onto the other. After 10 minutes, a single E. maximus snail (subject) was placed in the center of the dish and the lid was then placed on top. After 20 minutes, the location of the subject was recorded. This procedure was repeated for all 32 subjects, and the percentage of subjects on the side of the filter paper with the hexane extract solution (attraction rate) was calculated. The results are shown in Table 1. These results demonstrate that E. maximus snails are attracted to the hexane extract solution.

[0050] The attraction rate was calculated based on the following formula.

[0051]

number

[0052] [Table 1]

[0053] (3) Gas chromatograph mass spectrometry (GC / MS analysis) The hexane extract solution, which was confirmed to have an attractant effect by the bioassay described above, was subjected to GC / MS analysis.

[0054] The fatty acids, fatty acid esters, and fatty acid amides detected in the chromatograph were chemically identified by mass spectrometry.

[0055] (Gas chromatograph conditions) Measurement equipment: Agilent 6890N, 5793N (Agilent Technologies) Column: HP-5MS (Agilent Technologies) Temperature program: Set the temperature rise rate from 50℃ to 250℃ at 10℃ / min Injection mode: Splitless mode ·Injection volume: 2μL Carrier gas: Helium Ionization method: Electron ionization Scan mass: m / z 50~1000

[0056] (4) Bioassay of the attractant activity of the identified compounds Each of the compounds identified above was used as a standard to conduct an assay to detect whether the volatile gases from the solvent solutions of the standard compounds would attract the snail. The concentrations of the compounds and the solvents are as shown in Table 2. The assay was carried out in the same manner as in Example 1(2), and the attraction rate was calculated. The results are shown in Table 2.

[0057] The results in Table 2 clearly show that the fatty acid esters have an excellent attracting effect on the giant snail.

[0058] [Table 2]

[0059] Example 2: Identification of compounds with attractant activity against the small-headed snail (1) Hexane extraction of secretory substances A hexane extract solution of the small white-tailed snail was collected in the same manner as in Example 1 (1).

[0060] (2) Bioassay of attractant activity of hexane extract solution A bioassay of the hexane extract solution of the S. punctata was carried out in the same manner as in Example 1 (2). The results are shown in Table 3. The results demonstrate that the S. punctata is attracted to the hexane extract solution.

[0061] [Table 3]

[0062] (3) Gas chromatograph mass spectrometry (GC / MS analysis) The hexane extract solution, which was confirmed to have an attractant effect by the bioassay, was subjected to GC / MS analysis in the same manner as in Example 1 (3).

[0063] (4) Bioassay of the attractant activity of the identified compounds Each of the compounds identified above was used as a standard to conduct an assay to determine whether the volatile gases from a solvent solution of the standard attracted the snail. The compound concentrations and solvents are as shown in Table 4. For mixtures tested, the compounds were prepared so that the concentrations shown in Table 4 were used. The assay was performed in the same manner as in Example 1(2), and the attraction rate was calculated. The results are shown in Table 4.

[0064] The results in Table 4 reveal that fatty acids, fatty acid esters, and fatty acid amides have excellent attractant effects against the small-headed snail.

[0065] [Table 4]

[0066] Example 3 In the test in Example 2, a sample was prepared by adding 0.1 microliters of compound to a 5% by mass dimethyl sulfoxide (DMSO) solvent, and an assay was performed to detect whether the volatile gas from the sample attracted E. maxima and E. maxima to the sample. The types of compounds are listed in Table 5. The assay was performed in the same manner as in Example 1(2), and the attraction rate was calculated. The results are shown in Table 5.

[0067] The results in Table 5 reveal that fatty acid esters and fatty acid amides have excellent attractant effects on the giant salamander and the small white salamander.

[0068] [Table 5]

[0069] Example 4: Changes in food intake Synthetic feed 1 (cat food, oyster shells, and oatmeal mixed in a 2:2:1 ratio) and synthetic feed 2 (synthetic feed 1 to which an attractant (methyl palmitate) was added at a weight ratio of 0.1%) were compared. One giant snail was placed in each of 15 90 mm diameter petri dishes to create a feeding environment, and the amount of food consumed over a four-day period was compared. Compared to synthetic feed 1 without the added attractant, synthetic feed 2 with the added attractant showed a 39% increase in the average amount of food consumed per gram of live weight of giant snail. This demonstrates the attractive effect of the attractant of the present invention.

[0070] <Example 5: Slaughter test in an orchard using an attractant composition containing an insecticidal component> Slug and snail repellent, Sluggo R Attractive and insecticidal composition 1 was prepared by mixing 400 g of the insecticide (manufactured by Mitsui Chemicals Agro, Inc., active ingredient: ferric phosphate hydrate 0.98% by mass) with 0.4 g of an attractant (methyl palmitate) in a mixer.

[0071] In the orchard test, potted Taguchi Wase (Satsuma mandarin) 3-year-old trees (without fruit) were used as test trees in a glass greenhouse, and the Attractant Insecticide Composition 1 or Slag R m2 The insecticide composition was administered at a dose of 5 g per pot (0.89 g per container). Thirty giant salamanders were released per pot, and after 7 days, all of the giant salamanders were collected and assessed for viability. The giant salamanders were placed in plastic cups filled with shallow water, the lid was placed on top, and viability was assessed by determining whether the soft body emerged from the shell. Mortality was calculated as the number of confirmed dead snails divided by the number of released snails minus the number of snails that could not be confirmed alive or dead, and expressed as a percentage. The mortality rate at the time of investigation after 7 days was 8.5% higher in the plots administered Attractive Insecticide Composition 1 than in the plots administered Slaggo, which means that the slaughter effect of Slaggo (mortality rate of 50%) had increased by 17.0%.

[0072] These results demonstrate that fatty acid esters also have an excellent attracting effect on the giant snail in insecticidal compositions.

Claims

1. A composition for attracting terrestrial snails, comprising at least one selected from the group consisting of a fatty acid having a carbon number of 16 to 30 in the fatty acid moiety, a fatty acid ester having a carbon number of 14 to 30 in the fatty acid moiety, and a fatty acid amide having a carbon number of 14 to 30 in the fatty acid moiety, the fatty acid ester is at least one selected from the group consisting of methyl esters and ethyl esters, A composition for attracting terrestrial snails (excluding those containing oleic acid), wherein the terrestrial snail is a giant land snail or a small white land snail.

2. A terrestrial snail attractant composition as described in claim 1, further containing an insecticidal component that is effective against said terrestrial snails.

3. 2. The terrestrial snail attractant composition of claim 1, comprising at least one selected from the group consisting of palmitic acid, linoleic acid, stearic acid, methyl palmitate, methyl linoleate, methyl oleate, methyl stearate, ethyl palmitate, ethyl linoleate, ethyl oleate, ethyl stearate, palmitamide, linoleamide, oleamide, stearamide, erucamide, palmitoylethanolamide, and oleylethanolamide.

4. A trap for land snails comprising the land snail attractant composition of claim 1.

5. 10. A method of attracting land snails, comprising the step of placing the land snail attracting composition of claim 1 or the land snail trap of claim 4.

6. 6. The method for attracting plants according to claim 5, wherein the plants are placed on a predicted route that the land snails will take to contact the plant, or inside or around a field where the plant is cultivated.

Images