Muscle-building supplements

Abstract

A muscle enhancer containing a polyamine and a branched-chain amino acid as active ingredients.

Description

[Technical Field]

[0001] The present invention relates to a muscle-building agent. [Background technology]

[0002] It is believed that both proper nutritional intake and exercise are necessary for muscle building, that is, for increasing and maintaining muscle mass and strength, and for preventing the decline of muscle mass and strength. From the perspective of nutritional intake, it is believed that improving the absorption rate relative to the amount of nutrients ingested and improving the efficiency of the body's function of converting absorbed peptides and amino acids into muscle proteins are important for muscle building.

[0003] Steroids and growth hormones are commonly used as muscle-building agents, including anabolic steroids (protein-anabolic steroids) such as testosterone cypionate and methyltestosterone. However, these muscle-building agents may affect parts of the body other than the muscles, and may also cause side effects such as increased blood pressure and cholesterol levels and liver damage.

[0004] For this reason, research and development of muscle-building agents that focus particularly on safety has been conducted, and the use of olive extracts, for example, has been reported (e.g., Patent Document 1). If muscle-building agents that focus on safety could be provided, they would likely be used in a wide range of applications, such as improving athletic performance in sports, preventing or ameliorating obesity and metabolic syndrome, and slimming down. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Special Publication No. 2010-505784 Summary of the Invention [Problem to be solved by the invention]

[0006] The present invention has been made in consideration of the above problems, and aims to provide a novel muscle-building agent that is expected to improve the absorption rate relative to the amount of nutrient intake and to improve the efficiency of the biological function of converting absorbed peptides and amino acids into muscle proteins. [Means for solving the problem]

[0007] The present inventors have conducted extensive research to solve the above problems, and as a result have found that the above problems can be solved by using a polyamine and a branched-chain amino acid in combination, thereby completing the present invention.

[0008] That is, the present invention is as follows. [1] Contains polyamines and branched-chain amino acids as active ingredients. Muscle-enhancing agent. [2] The polyamine is one or more selected from the group consisting of putrescine, spermidine and spermine; The muscle-enhancing agent described in [1]. [3] Contains 1mg / g or more of spermidine per 1g of muscle-building supplement. The muscle-enhancing agent described in [2]. [4] The polyamines are derived from yeast. The muscle-building agent according to any one of [1] to [3]. [5] Polyamines include polyamine-containing yeasts. The muscle-enhancing agent described in [4]. [6] Contains 200-800mg of polyamine-containing yeast as dry mass per 1g of muscle-building supplement. The muscle-enhancing agent described in [5]. [7] Contains spermidine at a dry mass of 6.0% or less moisture at 1 mg / g or more. The muscle-enhancing agent according to [5] or [6]. [8] Further comprising a crystalline polysaccharide, The muscle-building agent according to any one of [5] to [7]. [9] The mass ratio of yeast to crystalline polysaccharide is 1:0.1 to 1; The muscle-enhancing agent described in [8].

[10] The yeast is a yeast belonging to the genus Saccharomyces, The muscle-building agent according to any one of [4] to [9].

[11] The branched chain amino acid is one or more selected from the group consisting of L-valine, L-leucine, and L-isoleucine. The muscle-building agent according to any one of [1] to

[10] .

[12] When the total of L-valine, L-leucine, and L-isoleucine is 100 parts by mass, Comprising 20 to 30 parts by mass of L-valine, 40 to 60 parts by mass of L-leucine, and 20 to 30 parts by mass of L-isoleucine, The muscle-enhancing agent according to

[11] .

[13] Contains 200-800mg of branched chain amino acids per 1g of muscle-building supplement, as a dry mass. The muscle-building agent according to any one of [1] to

[12] .

[14] [1] to

[13] , comprising the muscle-building agent according to any one of [1] to

[13] . composition.

[15] In the form of food and beverages, The composition described in

[14] . [Effects of the Invention]

[0009] According to the present invention, a novel muscle-building agent can be provided that is expected to improve the amount of absorption relative to the amount of nutrient intake and to improve the efficiency of the biological function of converting absorbed peptides and amino acids into muscle proteins. DETAILED DESCRIPTION OF THE INVENTION

[0010] Below, we will explain in detail the embodiment of the present invention (hereinafter referred to as the ``present embodiment''), but the present invention is not limited to this and various modifications are possible within the scope of the gist of the present invention.

[0011] [Muscle-enhancing agent] The muscle-building agent of this embodiment contains polyamine and branched-chain amino acid as active ingredients, and may contain other additives such as crystalline polysaccharides as needed.

[0012] Polyamines, which are linear aliphatic hydrocarbons in vivo with two or more primary amino groups, are highly effective in activating cells, and foods containing them are available. Polyamines are known to have physiological effects such as cell proliferation, cell differentiation promotion, immune essential factor, anti-allergic effect, protein synthesis promotion, structural stabilization through interaction with nucleic acids, and enzyme activity regulation. It has now been discovered that the combined use of such polyamines with branched-chain amino acids can provide novel muscle-building agents. Each component is described in detail below.

[0013] (Polyamine) Polyamine is a general term for straight-chain aliphatic hydrocarbons with multiple amino groups bonded thereto. Examples include putrescine, spermidine, spermine, 1,3-diaminopropane, cadaverine, caldine, homospermidine, aminopropylcadaverine, thermine, thermospermine, canavalmine, aminopentylnorspermidine, N,N-bis(aminopropyl)cadaverine, homospermine, caldopentamine, homocaldopentamine, caldohexamine, and homocaldohexamine spermidine. Among these, putrescine, spermidine, and spermine are preferred. These polyamines have high cell activation potential, high bioavailability, and excellent functionality. Spermidine, in particular, has a higher acceptable daily intake than spermine, making it preferable for larger doses. Furthermore, the use of these polyamines tends to further enhance the muscle-building effects of combined use with branched-chain amino acids.

[0014] The polyamine content per 1 g of the muscle-building agent is preferably 1 mg / g or more, more preferably 1 to 10 mg / g, and even more preferably 1 to 5 mg / g. When the polyamine content is within the above range, the muscle-building effect tends to be further improved. The polyamine content is not limited to the above, and can be within the range of the acceptable daily intake of a specific compound such as spermidine.

[0015] Methods for producing polyamines are not particularly limited, and known examples include a method for preparing polyamines by treating yeast cells or a yeast culture solution under acidic conditions, a method for extracting polyamines by treating a plant material under acidic conditions, a method for producing a polyamine extract by adding a salt solution such as table salt, magnesium chloride, or calcium chloride to a plant material, and a method for obtaining polyamine-containing yeast as exemplified in WO 2018 / 168525.

[0016] Among these, the polyamines used in this embodiment are preferably derived from yeast, and it is more preferable to use polyamine-containing yeast as the polyamine. Furthermore, yeast belonging to the genus Saccharomyces, preferably Saccharomyces cerevisiae, can be used as the yeast. The use of such polyamines tends to further improve the muscle-building effect. In this case, the polyamine content can be 1.5 mg or more, preferably 2.0 mg or more, and more preferably 2.5 mg or more per gram (dry mass) of yeast.

[0017] In this embodiment, unless otherwise specified, the term "dry mass" refers to the mass when the moisture content is 6.0% or less.

[0018] Dry yeast containing polyamines can be obtained by removing the yeast cells from a culture medium containing the yeast using filtration or a centrifuge and drying them. Washing may be performed to remove medium components. Spray drying, vacuum drying, freeze drying, etc. can be used to dry the yeast, and those skilled in the art can select an appropriate drying method depending on the purpose.

[0019] The polyamine contained in the muscle-building agent may be added as a polyamine compound or as the above-mentioned polyamine-containing yeast. Among these, it is preferable to add it as a polyamine-containing yeast.

[0020] Furthermore, when polyamines are added to a muscle-building agent as polyamine-containing yeast, the dry mass thereof is preferably 200 to 800 mg, more preferably 300 to 700 mg, and even more preferably 400 to 600 mg per gram of muscle-building agent. When the dry mass of the polyamine-containing yeast is within the above range, the muscle-building effect tends to be further improved. The content of polyamines is not limited to the above, and can be within the range of the acceptable daily intake of specific compounds such as spermidine.

[0021] Furthermore, when added as polyamine-containing yeast, the spermidine content per 1 g of muscle-building agent is preferably 0.8 mg / g or more, more preferably 0.9 mg / g or more, and even more preferably 1 mg / g or more, in a dry mass with a moisture content of 6.0% or less. When the dry mass of the polyamine-containing yeast is within the above range, the muscle-building effect tends to be further improved. Furthermore, when added as polyamine-containing yeast, the upper limit of the spermidine content is not particularly limited, but is preferably 10 mg / g or less, more preferably 5 mg / g or less.

[0022] (branched-chain amino acids) Branched-chain amino acids (BCAAs) are a collective term for one or more amino acids selected from the group consisting of L-valine, L-leucine, and L-isoleucine, which are among the nine essential amino acids that cannot be synthesized in the human body. They are important nutrients that serve as an energy source for muscles. Branched-chain amino acids are found in large amounts in meat, fish, dairy products, eggs, etc., but because they are ingested as protein from food, it takes several hours for them to be broken down into branched-chain amino acids and absorbed. Therefore, to efficiently absorb branched-chain amino acids, it is effective to ingest them as they are.

[0023] The muscle-building agent of this embodiment preferably contains L-valine, L-leucine, and L-isoleucine as branched-chain amino acids. In this case, the mass ratio of L-valine, L-leucine, and L-isoleucine is preferably 20 to 30 parts by mass of L-valine, 40 to 60 parts by mass of L-leucine, and 20 to 30 parts by mass of L-isoleucine, relative to 100 parts by mass of the total of L-valine, L-leucine, and L-isoleucine. By containing branched-chain amino acids in such a mass ratio, the muscle-building effect tends to be further improved.

[0024] The content of branched-chain amino acids contained in the muscle-building agent of this embodiment is preferably 200 to 800 mg, more preferably 300 to 700 mg, and even more preferably 400 to 600 mg, in dry mass per gram of muscle-building agent. When the content of branched-chain amino acids is within the above range, the muscle-building effect tends to be further improved.

[0025] The method for producing branched-chain amino acids is not particularly limited, and they can be produced by fermentation, synthesis, purification, etc., and commercially available products can be used. The branched-chain amino acids used in the present invention can be any of these common branched-chain amino acids.

[0026] (crystalline polysaccharide) The muscle-building agent of this embodiment may contain crystalline polysaccharides. The inclusion of crystalline polysaccharides can impart fluidity and deodorizing properties to the muscle-building agent, as well as impart stability to polyamines and polyamine-containing yeast. It is also believed that the inclusion of crystalline polysaccharides can suppress fat absorption, contributing to muscle-building effects.

[0027] Crystalline polysaccharides include, but are not limited to, polysaccharides that exist naturally as crystalline solids, such as starch, dextrin, glycogen, and cellulose. Among these, cyclodextrin, a dextrin with a cyclic structure, can be preferably used as a crystalline polysaccharide. There are alpha-, beta-, and gamma-type cyclodextrins, all of which can be used as crystalline polysaccharides. Among these, alpha-cyclodextrin and gamma-cyclodextrin are preferred. Other dextrins, such as indigestible dextrin, can also be used in the composition of the present invention.

[0028] Combining polyamine-rich yeast with crystalline polysaccharides stabilizes the yeast and makes it easier to handle. The yeast and crystalline polysaccharides can be mixed by either dry mixing or wet mixing. When wet mixing is used, the sugars can be sterilized, mixed with the yeast, and then dried. Alternatively, the yeast can be mixed as wet yeast rather than as dried yeast, and then dried. Furthermore, the yeast can be cultured, collected and concentrated by centrifugation, and crystalline polysaccharides can be added thereto, and then spray-dried to mix the yeast and crystalline polysaccharides.

[0029] The content of the crystalline polysaccharide per gram of the muscle-building agent is preferably 30 to 500 mg, more preferably 40 to 400 mg, and even more preferably 50 to 300 mg. When the content of the crystalline polysaccharide is within the above range, the muscle-building effect tends to be further improved.

[0030] Furthermore, when polyamine-containing yeast is used, the mass ratio of the dry mass of the polyamine-containing yeast to the crystalline polysaccharide is preferably 1:0.1 to 3, more preferably 1:0.1 to 2, and even more preferably 1:0.1 to 1. When the mass ratio of the dry mass of the polyamine-containing yeast to the crystalline polysaccharide is within the above range, the stability of the polyamine-containing yeast is further improved, and the content of polyamines, particularly spermidine, in the muscle-building agent can be ensured to a certain extent, so that the effects of the muscle-building agent can be obtained with an appropriate intake amount.

[0031] [Composition] The composition of the present embodiment contains the muscle-building agent. Its uses are not particularly limited, but it can be used, for example, in not only various general foods and beverages, but also foods and beverages, medicines, quasi-drugs, and cosmetics that are labeled with functional properties so that they can be taken by individuals who need nutritional reinforcement, particularly individuals who need cell activation.

[0032] Such foods and beverages are not particularly limited, but examples include foods and beverages for specified health uses, foods and beverages with nutritional functions, functional skin foods and beverages, foods and beverages with health functions, foods and beverages for special purposes, nutritional supplement foods and beverages, health supplement foods and beverages, supplements, beauty foods and beverages, and other health foods and beverages. [Example]

[0033] The present invention will be described in more detail below using examples and comparative examples, but the present invention is not limited to the following examples.

[0034] (Preparation of test food) (Capsule A) A BCAA preparation was prepared with branched-chain amino acids containing L-valine, L-leucine, and L-isoleucine in a mass ratio of 1:2:1. 123 mg of Elion SP (registered trademark), a spermidine-containing yeast food product manufactured by Mitsubishi Gas Chemical Company, Inc., was mixed with 112 mg of the prepared BCAA preparation and placed in a No. 2 hard capsule to prepare Capsule A. The spermidine-containing yeast in Elion SP (registered trademark) was adjusted to 100 mg in dry mass. The spermidine-containing yeast contained 3 mg of spermidine per 1 g of dry mass.

[0035] (Capsule B) 123 mg of corn starch and 112 mg of the prepared BCAA preparation were mixed and placed in a No. 2 hard capsule to prepare placebo capsule B.

[0036] (Capsule C) 235 mg of corn starch was placed in a No. 2 hard capsule to prepare capsule C for use as a placebo.

[0037] (Subject grouping and daily intake) Subjects who met the following conditions were selected as subjects, and the following tests were conducted. Healthy men aged 50 or older (in their 50s, 60s, or 70s) who are not receiving treatment for a disease - Those with a BMI of 18.5 or more and less than 30 -Those who have not taken supplements that may affect muscles (BCAA, protein, etc.) within one month prior to the start of the study -Those who do not consume natto, which is rich in polyamines, at least twice a week

[0038] The subjects were screened before ingestion to determine whether they were eligible for the study. Based on the daily intake of capsules, the subjects were divided into three groups: Group I, Group II, and Group III. Group I: A group taking 3 capsules of capsule A and 6 capsules of capsule B per day Yeast intake 300mg / day, BCAA intake 1008mg / day Group II: 9 capsules of Capsule B per day Yeast intake: 0 mg / day, BCAA intake: 1008 mg / day Group III: A group taking 9 capsules of Capsule C per day Yeast intake: 0 mg / day, BCAA intake: 0 mg / day

[0039] BCAA supplements are already available on the market from many manufacturers, and many of them recommend a daily intake of more than 2000 mg. However, in order to determine the effects of polyamines, the intake of BCAA supplements in this evaluation was set at 1008 mg (approximately half the usual recommended intake).

[0040] (Test Method) A double-blind human oral clinical trial was conducted on subjects in groups I, II, and III. Specifically, each subject took a total of nine capsules within 30 minutes of breakfast each day for eight weeks. During the trial, subjects were asked to keep a daily record of their daily steps, exercise, and diet, which serve as indicators of exercise indices. Afterwards, each subject was tested using the same items as in the pre-test.

[0041] (Test items) Before and after the test, the subjects underwent blood tests, as well as physiological tests to measure muscle mass and SMI using a body composition analyzer. The definitions of each term are listed below (see the Tanita Corporation instruction manual). The body composition analyzer used was the MC-980A-N plus manufactured by Tanita Corporation. This body composition analyzer uses the bioelectrical impedance method. Muscle mass: The amount of tissue excluding fat mass and estimated bone mass, which helps maintain posture and The mass of muscle tissue (skeletal muscle, smooth muscle, and water content) that moves the heart SMI: Skeletal Muscle Mass Index. Total muscle mass of left arm, right arm, left leg, and right leg (kg) / height (m)^2

[0042] (analysis) The differences between each item measured before and after the test were evaluated using the Wilcoxon rank-sum test, a non-parametric test. In this evaluation, a p value of p<0.05(*) was considered statistically significant, and a p value of p<0.01(**) was considered highly significant.

[0043] In addition, the correlation coefficient (r) between the intake of Elion SP (registered trademark) and the difference between before and after the test was calculated for Groups I, II, and III. In this evaluation, an r value of 0.4≦|r|≦0.7 was considered to be "correlated (*)," and an r value of 0.7<|r|<1.0 was considered to be "highly correlated (**)."

[0044] (Result 1) Table 1 shows the results of the above analysis for subjects belonging to Groups I and II. Nonparametric testing showed no significant differences between Elion SP (registered trademark) intake and items related to muscle mass in Group I. However, correlation coefficients showed a correlation between Elion SP (registered trademark) intake and muscle mass.

[0045] [Table 1]

[0046] (Result 2) Table 2 shows the results of performing statistical calculations again based on Group III, with the difference before and after the test set to 0. As a result, nonparametric testing revealed significant differences between Elion SP (registered trademark) intake and muscle mass and SMI in Group I, but no significant differences were found between Elion SP (registered trademark) intake and items related to muscle mass in Group II.

[0047] [Table 2]

[0048] (Result 3) Table 3 shows the results of the above analysis when the subjects in Groups I and II were further divided into "exercisers" and "non-exercisers."

[0049] The definition of "exercisers" is one whose average number of steps per day is equal to or greater than the literature value for the average number of steps for each age group, and includes those on the border of the second quartile when divided into quartiles. The reference was taken from "National Nutrition Survey 2017, Table 61" posted on the Ministry of Health, Labor and Welfare website.

[0050] The results showed that there was a significant difference in muscle mass and SMI in Group I, and the correlation coefficient also showed a correlation between muscle mass and SMI.

[0051] [Table 3] [Industrial Applicability]

[0052] INDUSTRIAL APPLICABILITY The present invention has industrial applicability as it provides a new muscle-building agent.

Claims

1. It contains polyamines, branched chain amino acids, and crystalline polysaccharides as active ingredients, The muscle-building agent contains 1 mg / g or more of spermidine per 1 g, The polyamine includes a polyamine-containing yeast, The polyamine-containing yeast contains spermidine as the polyamine, The polyamine-containing yeast is contained in an amount of 200 to 800 mg per 1 g of the muscle-building agent in terms of dry mass, The branched chain amino acid is contained in an amount of 200 to 800 mg per 1 g of the muscle-building agent as a dry mass. Muscle-enhancing agent.

2. The polyamine further comprises one or more selected from the group consisting of putrescine and spermine; The muscle-building agent according to claim 1.

3. The spermidine is contained in an amount of 1 mg / g or more in a dry mass having a moisture content of 6.0% or less. The muscle-building agent according to claim 1 or 2.

4. The mass ratio of yeast to crystalline polysaccharide is 1:0.1 to 1; The muscle-building agent according to claim 1.

5. The yeast is a yeast belonging to the genus Saccharomyces; The muscle-building agent according to any one of claims 1 to 4.

6. The branched chain amino acid is one or more selected from the group consisting of L-valine, L-leucine, and L-isoleucine. The muscle-building agent according to any one of claims 1 to 5.

7. When the total amount of L-valine, L-leucine, and L-isoleucine is 100 parts by mass, comprising 20 to 30 parts by mass of L-valine, 40 to 60 parts by mass of L-leucine, and 20 to 30 parts by mass of L-isoleucine; The muscle-building agent according to claim 6.

8. The muscle-building agent according to any one of claims 1 to 7, composition.

9. In the form of food and beverages, The composition of claim 8.

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