Skin sensitization measurement reagent, compound, and method for measuring skin sensitization

Abstract

The present invention addresses the problem of providing: a reagent that is for measuring skin sensitization and by which sensitization to a test substance can be measured with high sensitivity using a single reagent; a compound; and a method for measuring skin sensitization. The present invention provides: a reagent that is for measuring skin sensitization and that contains, as a main measuring drug, an organic compound having a mercapto group and the hydrazide structure and having an absorption spectrum in the ultraviolet, visible, or infrared region; a compound that is used in the reagent for measuring skin sensitization; and a method for measuring skin sensitization using the reagent for measuring skin sensitization.

Description

[Technical Field]

[0001] The present invention relates to a skin sensitization measurement reagent, a compound, and a method for measuring skin sensitization. [Background technology]

[0002] Skin sensitization (allergy) not only causes localized symptoms such as blisters and erythema at the site of exposure to a substance, but can also be accompanied by a severe, life-threatening systemic allergic reaction called anaphylaxis. Furthermore, once skin sensitization has developed, it is necessary to manage exposure by avoiding it for a long period of time, and therefore it is considered to be one of the most important types of toxicity.

[0003] Test methods using guinea pigs have traditionally been well known for assessing the skin sensitization potential of chemicals, and test methods such as the Guinea Pig Maximization Test (GPMT), which uses an adjuvant, and the Buehler Test, which is a non-adjuvant test, have been widely used for many years. However, in recent years, research and development of alternative methods to animal testing has been progressing due to ethical and social demands such as animal welfare.

[0004] Development of in vitro tests is currently underway as a non-animal skin sensitization test method. TM Known methods include the ARE-NrF2 lusiferase test method (KeratinoSens is a registered trademark), LuSens (ARE-NrF2 lusiferase LuSens test method), h-CLAT (human Cell Line Activation Test), U-SENS (Myeloid U937 Skin Sensitization Test), and IL-8 Luc assay.

[0005] On the other hand, there is the in chemico test, a test method that does not use cultured cells. In chemico tests using chemical reactions have many advantages, such as not requiring special techniques, knowledge, or equipment because they do not use cultured cells. For example, Non-Patent Documents 1 and 2 describe a method that uses two types of peptides (cysteine ​​peptides and lysine peptides) as nucleophilic reagents. Patent Documents 1 and 2 also describe a skin sensitization measurement reagent and a skin sensitization measurement method that use a cysteine ​​derivative with an aryl ring and a lysine derivative with an aryl ring as nucleophilic reagents (also referred to as ADRA).

[0006] In the methods described in Non-Patent Documents 1 and 2 and Patent Documents 1 and 2, two types of reagents containing cysteine ​​and lysine are separately chemically reacted with the test substance, and then measured and quantified separately to calculate the depletion rate, resulting in a time-consuming evaluation process. Therefore, test methods have also been reported that use peptides containing these two amino acids to detect and evaluate skin sensitizers. However, both test methods use the synthetic heptapeptide Cor1C-420 (Ac-Asn-Lys-Lys-Cys-Asp-Leu-Phe) (derived from the sequence surrounding the 420th cysteine ​​residue from the N-terminus of human Coronin 1 protein, a site that exhibits high reactivity with electrophilic reagents (Non-Patent Document 3)) and perform measurement by LC-MS (liquid chromatography-mass spectrometry). Because both methods have low detection sensitivity, optical detection using UV or visible light is not possible. Furthermore, the inclusion of cysteine ​​and lysine does not address the shortened measurement time.

[0007] Non-Patent Document 4 describes five points: (1) peptide-analyte adducts (covalent conjugates) can be distinguished from peptide oxidation; (2) the test substance concentration in the reaction solution can be reduced, eliminating the problem of test substance precipitation; (3) the test substance preparation concentration can be reduced, reducing the problem of test substance solubility; (4) LC-MS measurement eliminates the problem of coelution; and (5) kinetic measurement allows for more accurate evaluation of highly reactive test substances. Non-Patent Document 5 also describes that high prediction accuracy can be achieved by evaluating three types of peptides: the cysteine ​​peptide and lysine peptide used in the Direct Peptide Reactivity Assay (DPRA) described in Non-Patent Documents 1 and 2, and the synthetic heptapeptide Cor1C-420 described in Non-Patent Document 3. Furthermore, Patent Document 3 describes a skin sensitization detection reagent in which a fluorescent dye is attached to the terminus of a peptide containing an amino group and a thiol group in the same molecule. [Prior art documents] [Non-patent literature]

[0008] [Non-Patent Document 1] Gerberick, GF, Vassallo, JD, Bailey, RE, Chaney, JG, Morrall, SW and Lepoittevin, JP (2004). Development of a peptide reactivity assay for screening contact allergens. Toxicological Sciences, 81(2), 332-43. [Non-patent document 2] Gerberick, G.F., Vassallo, J.D., Foertsch, L.M., Price, B.B., Chaney, J.G. and Lepoittevin, J.P. (2007). Quantification of chemical peptide reactivity for screening contact allergens: a classification tree model approach. Toxicological Sciences, 97(2), 417-27. [Non-Patent Document 3] Denny MK, Richards AM, Wemke GR, Shyr Y, Liebler DC. (2006) Cytosolic and nuclear protein targets of thiol-reactive electrophiles. Chemical Research in Toxicology, 19, 20-29. [Non-Patent Document 4] Natsch A, Gfeller H. (2008). LC-MS-based characterization of the peptide reactivity of chemicals to improve the in vitro prediction of the skin sensitization potential. Toxicological Sciences, 106(2), 464-78. [Non-Patent Document 5] Wong CL, Lam AL, Smith MT, Ghassabian S. (2016). Evaluation of a High-Throughput Peptide Reactivity Format Assay for Assessment of the Skin Sensitization Potential of Chemicals. Frontiers in Pharmacology, 14, 7(53), 1-14. [Patent Document]

[0009] [Patent Document 1] Japanese Patent Application Laid-Open No. 2011-59102 [Patent Document 2] Japanese Patent Application Laid-Open No. 2014-37995 [Patent Document 3] Japanese Patent Application Laid-Open No. 2009-222466 Summary of the Invention [Problem to be solved by the invention]

[0010] In the skin sensitization measurement methods described in Non-Patent Documents 1 and 2, the molar extinction coefficients of the two peptides used are low and only detectable at a short wavelength of 220 nm. Therefore, quantifying the residual rates of these peptides using the HPLC-UV method often presents problems, such as low quantitative sensitivity and the likelihood of co-elution of the peptides with the test substance, making quantification difficult. The method described in Non-Patent Document 3 is problematic in that optical detection using UV or visible light is difficult, requiring evaluation by mass spectrometry. Furthermore, this test method does not claim that the inclusion of cysteine ​​and lysine shortens the measurement time. Non-Patent Document 4 does not describe the efficiency improvement achieved by shortening the measurement time. The method described in Non-Patent Document 5 has the problem of low optical quantitative sensitivity. Furthermore, the peptide used in the method described in Patent Document 3 contains a cysteine-derived thiol group and an amino acid α-amino group, but this peptide has low reactivity to test substances with weak skin sensitization, which can lead to false-negative results, which has been a problem.

[0011] An object of the present invention is to provide a skin sensitization measurement reagent, a compound, and a method for measuring skin sensitization, which are capable of measuring the sensitization of a test substance with high sensitivity using a single reagent. [Means for solving the problem]

[0012] As a result of intensive research to solve the above problems, the present inventors have found that an organic compound having a mercapto group and a hydrazide structure and having an absorption spectrum in the ultraviolet, visible light, or near-infrared region can be used as a reagent for measuring skin sensitization, and have completed the present invention. According to the present invention, the following inventions are provided.

[0013] <1> A skin sensitization measurement reagent comprising, as a measurement agent, an organic compound having a mercapto group and a hydrazide structure and having an absorption spectrum in the ultraviolet, visible light or near-infrared region. <2> The organic compound is represented by the following formula (1) or (2): <1> A skin sensitization measurement reagent according to claim 1. [ka] During the ceremony, A 1 represents a nitrogen atom or the following linking group: [ka] R 11 , R 12 , R 13 and R 14 each independently represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, an alkenyl group having 2 to 10 carbon atoms, an alkynyl group having 2 to 10 carbon atoms, a cycloalkyl group having 3 to 10 carbon atoms, or a cycloalkenyl group having 3 to 10 carbon atoms, and these do not include -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 1 -CO-, -CO-NJ 1 - or -NH-CO-NH-, J 1 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group, alkenyl group, alkynyl group, cycloalkyl group, or cycloalkenyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, a cycloalkenyl group having 5 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a mercaptomethyl group, a hydroxyl group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. * is X 1 , Y 1, Z 1 Indicates the connection position. X 1 and X 2 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, an alkenyl group having 2 to 10 carbon atoms and having one or more mercapto groups, an alkynyl group having 2 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, a cycloalkenyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- in the molecular chain. 2 -CO-, -CO-NJ 2 - or -NH-CO-NH-, J 2 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group, alkenyl group, alkynyl group, cycloalkyl group, or cycloalkenyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, a cycloalkenyl group having 5 to 6 carbon atoms, an amino group, a cyano group, a mercaptomethyl group, a hydroxyl group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 1 and Y 2 represents a group having 6 to 20 carbon atoms and containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region. Z 1 and Z 2 is -CO-NR 21 NR 22 R 23 indicates R 21 , R 22 , and R 23 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. <3> The organic compound is represented by the following formula (10): <1> A skin sensitization measurement reagent according to claim 1. [ka] During the ceremony, A10 represents a nitrogen atom or a trivalent linking group, X 10 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, an alkenyl group having 2 to 10 carbon atoms and having one or more mercapto groups, an alkynyl group having 2 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, a cycloalkenyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- in the molecular chain. 101 -CO-, -CO-NJ 101 - or -NH-CO-NH-, J 101 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group, alkenyl group, alkynyl group, cycloalkyl group, or cycloalkenyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, a cycloalkenyl group having 5 to 6 carbon atoms, an amino group, a cyano group, a mercaptomethyl group, a hydroxyl group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 10 represents a group having 6 to 20 carbon atoms containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region, L represents an amino group. <4> The organic compound is represented by the following formula (3), (4) or (5): <1> A skin sensitization measurement reagent according to claim 1. [ka] During the ceremony, A 3 represents a trivalent hydrocarbon group having 1 or 2 carbon atoms, R 3 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 31 -CO-, -CO-NJ 31- or -NH-CO-NH-, J 31 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 3 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 32 -CO-, -CO-NJ 32 - or -NH-CO-NH-, J 32 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 3 represents a group having 6 to 20 carbon atoms and containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region. Z 3 is -CO-NR 31 NR 32 R 33 indicates R 31 , R 32 , and R 33 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. [ka] During the ceremony, A 4 represents a trivalent hydrocarbon group having 1 or 2 carbon atoms, R 4represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 41 -CO-, -CO-NJ 41 - or -NH-CO-NH-, J 41 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 4 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 42 -CO-, -CO-NJ 42 - or -NH-CO-NH-, J 42 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 4 represents a group having 6 to 20 carbon atoms and containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region. Z 4 is -CO-NR 41 NR 42 R 43 indicates R 41 , R 42 , and R 43 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. [ka] During the ceremony, A 5 represents a trivalent hydrocarbon group having 1 or 2 carbon atoms, R 5 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 51 -CO-, -CO-NJ 51 - or -NH-CO-NH-, J 51 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 5 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 52 -CO-, -CO-NJ 52 - or -NH-CO-NH-, J 52 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 5 represents a group having 6 to 20 carbon atoms and containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region. Z 5 is -CO-NR 51 NR 52 R 53 indicates R 51 , R 52 , and R 53are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. Q represents a hydrogen atom, a carboxyl group, a hydroxyl group, or a primary amide structure, or -CO-NR 5a NR 5b R 5c indicates R 5a , R 5b , and R 5c are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. <5> The organic compound is represented by the following formula (6): <1> A skin sensitization measurement reagent according to claim 1. [ka] During the ceremony, R 6 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 61 -CO-, -CO-NJ 61 - or -NH-CO-NH-, J 61 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 6 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 62 -CO-, -CO-NJ 62 - or -NH-CO-NH-, J 62represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Z 6 is -CO-NR 61 NR 62 R 63 indicates R 61 , R 62 , and R 63 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. n represents 0 or 1, m represents 0 or 1. <6> The organic compound is represented by the following formula (7): <1> A skin sensitization measurement reagent according to claim 1. [ka] During the ceremony, R 7 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 71 -CO-, -CO-NJ 71 - or -NH-CO-NH-, J 71 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 7represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 72 -CO-, -CO-NJ 72 - or -NH-CO-NH-, J 72 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. W is NR 71 -NR 72 R 73 indicates R 71 , R 72 , and R 73 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, n indicates 0 or 1. <7> Y 1 , Y 2 , Y 3 , Y 4 , Y 5 , and Y 10 is a fluorescent group, <2> from <4> 10. The skin sensitization measurement reagent according to any one of the above. <8> A compound represented by the following formula (3), (4) or (5): [ka] During the ceremony, A 3 represents a trivalent hydrocarbon group having 1 or 2 carbon atoms, R 3 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 31 -CO-, -CO-NJ 31- or -NH-CO-NH-, J 31 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 3 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 32 -CO-, -CO-NJ 32 - or -NH-CO-NH-, J 32 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 3 represents a group having 6 to 20 carbon atoms and containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region. Z 3 is -CO-NR 31 NR 32 R 33 indicates R 31 , R 32 , and R 33 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. [ka] During the ceremony, A 4 represents a trivalent hydrocarbon group having 1 or 2 carbon atoms, R 4represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 41 -CO-, -CO-NJ 41 - or -NH-CO-NH-, J 41 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 4 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 42 -CO-, -CO-NJ 42 - or -NH-CO-NH-, J 42 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 4 represents a group having 6 to 20 carbon atoms and containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region. Z 4 is -CO-NR 41 NR 42 R 43 indicates R 41 , R 42 , and R 43 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. [ka] During the ceremony, A 5 represents a trivalent hydrocarbon group having 1 or 2 carbon atoms, R 5 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 51 -CO-, -CO-NJ 51 - or -NH-CO-NH-, J 51 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 5 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 52 -CO-, -CO-NJ 52 - or -NH-CO-NH-, J 52 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 5 represents a group having 6 to 20 carbon atoms and containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region. Z 5 is -CO-NR 51 NR 52 R 53 indicates R 51 , R 52 , and R 53are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. Q represents a hydrogen atom, a carboxyl group, a hydroxyl group, or a primary amide structure, or -CO-NR 5a NR 5b R 5c indicates R 5a , R 5b , and R 5c are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. <9> A compound represented by the following formula (6): [ka] During the ceremony, R 6 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 61 -CO-, -CO-NJ 61 - or -NH-CO-NH-, J 61 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 6 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 62 -CO-, -CO-NJ 62 - or -NH-CO-NH-, J 62represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Z 6 is -CO-NR 61 NR 62 R 63 indicates R 61 , R 62 , and R 63 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. n represents 0 or 1, m represents 0 or 1. <10> A compound represented by the following formula (7): [ka] During the ceremony, R 7 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 71 -CO-, -CO-NJ 71 - or -NH-CO-NH-, J 71 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 7 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 72-CO-, -CO-NJ 72 - or -NH-CO-NH-, J 72 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. W is NR 71 -NR 72 R 73 indicates R 71 , R 72 , and R 73 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, n indicates 0 or 1. <11> (1) <1> from <7> reacting a test substance with the skin sensitization measurement reagent described in any one of the above items; and (2) detecting the amount of the skin sensitization measurement reagent after the reaction or the amount of the reaction product by optical measurement; A method for measuring skin sensitization, including: <12> The test substance is at least one of fragrances, essential oils, polymer compounds, pharmaceuticals, pesticides, foods, chemical products, and plant extracts containing naturally occurring ingredients; <11> A method for measuring skin sensitization according to claim 1. <13> chromatographically treating the reaction product obtained in the step of reacting the skin sensitization measurement reagent with the test substance. <11> or <12> A method for measuring skin sensitization according to claim 1. <14> The optical measurement is a measurement using a fluorescence detector, and the excitation wavelength is 200 to 600 nm and the fluorescence wavelength is 200 to 800 nm. <11> from <13> 1. A method for measuring skin sensitization according to any one of the preceding claims. [Effects of the Invention]

[0014] According to the present invention, the sensitization potential of a test substance can be measured with high sensitivity using a single type of reagent. [Brief explanation of the drawings]

[0015] [Figure 1] FIG. 1 shows the results of calculating the residual rate of Compound 1 immediately after the solution preparation (0 hours) and after 24 hours. [Figure 2] FIG. 2 shows the results of measuring the fluorescence intensity (peak area in HPLC) of Compound 1 immediately after the solution preparation (0 hours). [Figure 3] FIG. 3 shows the results of comparing the depletion of each nucleophilic reagent in substances No. 1 to 8 listed in Table 2. [Figure 4] FIG. 4 shows the results of comparing the depletion of each nucleophilic reagent in substances No. 9 to 15 listed in Table 2. DETAILED DESCRIPTION OF THE INVENTION

[0016] In this specification, the symbol "to" is used to mean that the numerical values ​​before and after it are included as the lower limit and upper limit. In this specification, measurement of skin sensitization includes testing of skin sensitization, and also includes determining whether or not a substance has skin sensitization according to a certain standard, and quantitatively measuring skin sensitization.

[0017] It is important that chemicals contained in products such as pharmaceuticals, pesticides, and cosmetics do not cause skin sensitization, and a method for predicting the skin sensitization potential of chemicals is needed. Skin sensitization develops through a complex process consisting of many steps. The first event is the covalent binding of a test substance to proteins in the skin after penetration through the skin. Therefore, evaluating this covalent binding can be used to predict whether a test substance is a skin sensitizer. The reaction between skin proteins and a test substance is known to involve approximately five organic chemical reactions. The amino acids involved in these five reactions are the SH group of cysteine ​​and the NH2 group of lysine. Therefore, in the skin sensitization assay described in Patent Documents 1 and 2, two nucleophilic reagents are chemically synthesized, each containing a naphthalene ring with a high molar extinction coefficient in the UV range at the N-terminus of cysteine ​​and lysine. These two nucleophilic reagents are then reacted with the test substance, and the unreacted nucleophilic reagent is quantified to calculate the reactivity with the test substance and predict skin sensitization.

[0018] In the present invention, by using an organic compound having an absorption spectrum in the ultraviolet, visible light, or near-infrared region, it is possible to quantify the test substance at a dilute concentration of the evaluation reagent, thereby preventing precipitation due to poor solubility and improving quantitation. In the present invention, by using an organic compound having a mercapto group and a hydrazide structure in the same molecule, it has become possible to evaluate skin sensitization in a single operation. Since hydrazide groups are highly reactive to aldehyde-based test substances with low sensitizing properties, or the reaction products are highly stable, the present invention can reduce the false negative rate compared to conventional evaluation methods, which sometimes resulted in false negatives.

[0019] The skin sensitization measurement reagent of the present invention contains, as a measurement agent, an organic compound having a mercapto group and a hydrazide structure and having an absorption spectrum in the ultraviolet, visible light or near-infrared region.

[0020] The organic compound used in the present invention has an absorption spectrum in the ultraviolet, visible light, or near-infrared region, and is a compound that, in its pure state or in solution, preferably exhibits absorption in the wavelength region of 190 to 2500 nm, more preferably exhibits absorption in the wavelength region of 200 to 700 nm.

[0021] The organic compound used in the present invention is preferably a compound that emits light at 200 to 800 nm, more preferably a compound that emits light at 200 to 700 nm, and even more preferably a compound that emits light at 250 to 650 nm.

[0022] The organic compound used in the present invention is preferably a compound represented by the following formula (1) or (2), more preferably a compound represented by the following formula (1).

[0023] [ka] During the ceremony, A 1 represents a nitrogen atom or the following linking group: [ka] R 11 , R 12 , R 13 and R 14 each independently represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, an alkenyl group having 2 to 10 carbon atoms, an alkynyl group having 2 to 10 carbon atoms, a cycloalkyl group having 3 to 10 carbon atoms, or a cycloalkenyl group having 3 to 10 carbon atoms, and these do not include -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 1 -CO-, -CO-NJ 1 - or -NH-CO-NH-, J 1represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group, alkenyl group, alkynyl group, cycloalkyl group, or cycloalkenyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, a cycloalkenyl group having 5 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a mercaptomethyl group, a hydroxyl group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. * is X 1 , Y 1 , Z 1 Indicates the connection position. X 1 and X 2 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, an alkenyl group having 2 to 10 carbon atoms and having one or more mercapto groups, an alkynyl group having 2 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, a cycloalkenyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- in the molecular chain. 2 -CO-, -CO-NJ 2 - or -NH-CO-NH-, J 2 represents an alkyl group having 1 to 3 carbon atoms, and the alkyl group, alkenyl group, alkynyl group, cycloalkyl group, or cycloalkenyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, a cycloalkenyl group having 5 to 6 carbon atoms, an amino group, a cyano group, a mercaptomethyl group, a hydroxyl group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 1 and Y 2 represents a group having 6 to 20 carbon atoms and containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region. Z 1 and Z 2 is -CO-NR 21 NR 22 R23 indicates R 21 , R 22 , and R 23 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms.

[0024] R 11 , R 12 , R 13 and R 14 are preferably each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. R 11 , R 12 , R 13 and R 14 is more preferably a hydrogen atom or a methyl group. R 11 , R 12 , R 13 and R 14 is particularly preferably a hydrogen atom.

[0025] X 1 and X 2 represents preferably an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these may have -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- groups in the molecular chain. 2 -CO-, -CO-NJ 2 - or -NH-CO-NH-, J 2 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms. Y 1 and Y 2 preferably represents a group having 10 to 20 carbon atoms containing a structure having an absorption spectrum in the ultraviolet, visible light or near infrared region. R 21 , R 22 , and R 23 is preferably a hydrogen atom.

[0026] The organic compound used in the present invention is more preferably a compound represented by the following formula (10).

[0027] [ka] During the ceremony, A 10 represents a nitrogen atom or a trivalent linking group, X 10 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, an alkenyl group having 2 to 10 carbon atoms and having one or more mercapto groups, an alkynyl group having 2 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, a cycloalkenyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- in the molecular chain. 101 -CO-, -CO-NJ 101 - or -NH-CO-NH-, J 101 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group, alkenyl group, alkynyl group, cycloalkyl group, or cycloalkenyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, a cycloalkenyl group having 5 to 6 carbon atoms, an amino group, a cyano group, a mercaptomethyl group, a hydroxyl group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 10 represents a group having 6 to 20 carbon atoms containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region, L represents an amino group.

[0028] A 10 preferably represents a trivalent linking group, more preferably represents a trivalent linking group, [ka] Shows.

[0029] X 10represents preferably an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these may have -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- groups in the molecular chain. 101 -CO-, -CO-NJ 101 - or -NH-CO-NH-, J 101 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms. Y 10 preferably represents a group having 10 to 20 carbon atoms containing a structure having an absorption spectrum in the ultraviolet, visible light or near infrared region.

[0030] The structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region refers to the structure of a compound having absorption in the region of 200 nm to 2500 nm. Examples of compounds having absorption in the region of 200 nm to 2500 nm include naphthalene derivatives, anthracene derivatives, phenanthrene derivatives, tetracene derivatives, pentacene derivatives, benzopyrene derivatives, chrysene derivatives, pyrene derivatives, triphenylene derivatives, corannulene derivatives, coronene derivatives, ovalene derivatives, acridine derivatives, luciferin derivatives, pyranine derivatives, stilbene derivatives, benzofuran derivatives, dihydroquinoxalinone derivatives, phthalimidinyl derivatives, dansyl derivatives, merocyanine derivatives, perylene derivatives, acridine derivatives, perylene derivatives, luciferin derivatives, pyranine derivatives, stilbene derivatives, rhodamine derivatives, coumarin derivatives, and 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM). Examples of such derivatives include pyrromethene derivatives, fluorescein derivatives, umbelliferone derivatives, benzothiazole derivatives, benzoxadiazole derivatives, shikonin derivatives, fluoranthene derivatives, carbazole derivatives, tetraphene derivatives, chrysene derivatives, acenaphthene derivatives, and fluorene derivatives. Specifically, 2-naphthylacetyl chloride, 4-(5,6-dimethoxy-N-phthalimidinyl)benzenesulfonic acid chloride (DPS-CL), 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-CL), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RBITC), 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NDB-F), 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F), 4-(N-phthalimidinyl)benzenesulfonic acid chloride (PHISYL-CL), 4-aminosulfonyl-7-fluoro-2,1,3-Benzoxadiazole (ABD-F), N-[4-(6-dimethylamino-2-benzofuranyl)phenyl]maleimide (DBPM), 2-(4-maleimidophenyl)-6-methylbenzothiazole (MBPM), N-(9-acridinyl)maleimide (NAM), 4-chloro-7-sulfobenzofurazan ammonium salt (SBD-CL), 7-fluorobenzofurazan-4-sulfonic acid ammonium salt (SBD-F), 1,2-diamino-4,5-dimethoxybenzene (DDB), 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole (DBD-H), 4-hydrazino-7-nitro-2,1,3-benzoxadiazole hydrazine (DBD-H), 2,2′-dithiodi(1-naphthylamine) (DTAN), 4-amino-3-penten-2-one (FLUORAL-P), 1,2-amino-4,5-methylenedioxybenzene (MDB), 4-(5,6-dimethoxybenzothiazol-2-yl)benzoic acid hydrazide (BHBT), 4-(N,N-dimethylaminosulfonyl)-7-(N-hydrazinocarbonylmethyl-N-methyl)amino-2,1,3-benzoxadiazole (DBD-CO-HZ), 4-(N-hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole (NBD-CO-HZ), 3-bromomethyl-6,7-dimethoxy-1-methyl-1,2-dihydroquinoxalin-2-one (BR-DMEQ), 4-bromomethyl-7-methoxycoumarin (BR-MMC), 4-(N,N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ), 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ), 4-(N,N-dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole Examples include compounds derived from (DBD-ED), 3-chlorocarbonyl-6,7-dimethoxy-1-methyl-2(1H)-quinoxalinone (DMEQ-COCL), 2-(5-chlorocarbonyl-2-oxazolyl)-5,6-methylenedioxybenzofuran (OMB-COCL), etc.

[0031] The organic compound used in the present invention is more preferably a compound represented by the following formula (3), (4), or (5): According to the present invention, there is provided a compound represented by the following formula (3), (4), or (5):

[0032] [ka]

[0033] A 3 , A 4 , and A 5 represents a trivalent hydrocarbon group having 1 or 2 carbon atoms. R 3 , R 4 , and R 5 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 31 -CO-, -CO-NJ 31 - or -NH-CO-NH-, J 31 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 3 , X 4 and X 5 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 32 -CO-, -CO-NJ 32 - or -NH-CO-NH-, J 32represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Y 3 , Y 4 , and Y 5 represents a group having 6 to 20 carbon atoms containing a structure having an absorption spectrum in the ultraviolet, visible light, or near-infrared region. Z 3 is -CO-NR 31 NR 32 R 33 indicates R 31 , R 32 , and R 33 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. Z 4 is -CO-NR 41 NR 42 R 43 indicates R 41 , R 42 , and R 43 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. Z 5 is -CO-NR 51 NR 52 R 53 indicates R 51 , R 52 , and R 53 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. Q represents a hydrogen atom, a carboxyl group, a hydroxyl group, or a primary amide structure, or -CO-NR 5a NR 5b R 5c indicates R 5a , R 5b , and R 5c are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms.

[0034] A 3 , A 4 , and A 5 is preferably [ka] Shows. R 3 , R 4 , and R 5 preferably represents a hydrogen atom or an alkyl group having 1 to 10 carbon atoms, and more preferably represents a hydrogen atom or a methyl group. X 3 , X 4 and X 5 represents preferably an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these may have -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- groups in the molecular chain. 32 -CO-, -CO-NJ 32 - or -NH-CO-NH-, J 32 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms. Y 3 , Y 4 , and Y 5 preferably represents a group having 10 to 20 carbon atoms containing a structure having an absorption spectrum in the ultraviolet, visible light or near infrared region. R 21 , R 22 , and R 23 preferably represents a hydrogen atom. Q preferably represents a primary amide structure.

[0035] In the present invention, Y 1 , Y 2 , Y 3 , Y 4 , Y 5 , and Y 10 is preferably a fluorescent group.

[0036] The organic compound used in the present invention is more preferably a compound represented by the following formula (6): According to the present invention, there is provided a compound represented by the following formula (6).

[0037] [ka]

[0038] R 6 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 61 -CO-, -CO-NJ 61 - or -NH-CO-NH-, J 61 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 6 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 62 -CO-, -CO-NJ 62 - or -NH-CO-NH-, J 62 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Z 6 is -CO-NR 61 NR 62 R 63 indicates R 61 , R 62 , and R 63 are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. n represents 0 or 1, m represents 0 or 1.

[0039] R 6 preferably represents a hydrogen atom or a methyl group. X 6 represents preferably an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these may have -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- groups in the molecular chain. 62 -CO-, -CO-NJ 62 - or -NH-CO-NH-, J 62 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms. R 61 , R 62 , and R 63 preferably represents a hydrogen atom. n preferably represents 1. m preferably represents 0.

[0040] The organic compound used in the present invention is particularly preferably a compound represented by the following formula (7): According to the present invention, there is provided a compound represented by the following formula (7).

[0041] [ka]

[0042] R 7 represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 71 -CO-, -CO-NJ 71 - or -NH-CO-NH-, J 71represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X 7 represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. 72 -CO-, -CO-NJ 72 - or -NH-CO-NH-, J 72 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. W is NR 71 -NR 72 R 73 indicates R 71 , R 72 , and R 73 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, n indicates 0 or 1.

[0043] R 7 preferably represents a hydrogen atom or a methyl group. X 7 represents preferably an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these may have -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- groups in the molecular chain. 72 -CO-, -CO-NJ72 - or -NH-CO-NH-, J 72 represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms. R 71 , R 72 , and R 73 preferably represents a hydrogen atom. n preferably represents 1.

[0044] Examples of the alkyl group having 1 to 10 carbon atoms include a methyl group, an ethyl group, a propyl group, an isopropyl group, a butyl group, an isobutyl group, a sec-butyl group, a tert-butyl group, a pentyl group, an isopentyl group, a neopentyl group, a tert-pentyl group, a hexyl group, a heptyl group, an octyl group, a nonyl group, and a decyl group. Examples of the alkenyl group having 2 to 10 carbon atoms include a vinyl group, a 1-propenyl group, a 2-propenyl group, a 1-butenyl group, a 2-butenyl group, a 3-butenyl group, a 1,3-butenyl group, a 1-pentenyl group, a 1-hexenyl group, a 1-heptenyl group, a 1-octenyl group, a 1-nonenyl group, and a 1-decenyl group. Examples of the alkynyl group having 2 to 10 carbon atoms include an ethynyl group, a 1-propynyl group, a 1-butynyl group, a 1-pentynyl group, a 1-hexynyl group, a 1-heptynyl group, a 1-octynyl group, a 1-nonyl group, and a 1-decynyl group. Examples of the cycloalkyl group having 3 to 10 carbon atoms include a cyclopropyl group, a cyclobutyl group, a cyclopentyl group, a cyclohexyl group, a cycloheptyl group, a cyclooctyl group, a cyclononyl group, and a cyclodecyl group. Examples of the cycloalkenyl group having 3 to 10 carbon atoms include a cyclopropenyl group, a cyclobutenyl group, a cyclopentenyl group, a cyclohexenyl group, a cycloheptenyl group, a cyclooctenyl group, a cyclononenyl group, and a cyclodecenyl group. Examples of the arylalkyl group having 7 to 12 carbon atoms include a phenylmethyl group and a phenylethyl group. Examples of heteroalkylalkyl groups having 3 to 10 carbon atoms include the following structures. * indicates the point of attachment. When the structure has an asymmetric carbon, all possible stereoisomers are included. [ka]

[0045] <Method for synthesizing organic compounds> The organic compounds used in the present invention can be produced by chemical synthesis. For example, Compound 1 described in the Examples can be synthesized by reacting 1-naphthylacetic acid with S-trityl-L-cysteine ​​in the presence of 1,1'-carbonyldiimidazole to produce the intermediate N-(2-(naphthalen-1-yl)acetyl)-S-trityl-L-cysteine, and then reacting this intermediate with hydrazine monohydrate in the presence of 1,1'-carbonyldiimidazole.

[0046] Alternatively, the organic compounds used in the present invention can be produced using known peptide synthesis methods. Specifically, they can be produced in accordance with the methods described in the synthesis of compounds 6 to 12 and 14 in the Examples below. That is, the organic compounds used in the present invention can be synthesized by solid-phase peptide synthesis using a commercially available automated peptide synthesizer.

[0047] The synthesis can be carried out by setting up a solid-phase synthesis resin, an N-methyl-2-pyrrolidone (NMP) solution of Fmoc amino acids, an NMP solution of cyano-hydroxyimino-acetic acid ethyl ester and diisopropylethylamine, an NMP solution of diisopropylcarbodiimide, an NMP solution of piperidine, and an NMP solution of acetic anhydride in the synthesis apparatus. One cycle consists of Fmoc deprotection, washing with NMP, condensation of the Fmoc amino acids, and washing with NMP, and by repeating this cycle, the peptide chain can be elongated.

[0048] <Skin sensitization measurement reagent> The skin sensitization measurement reagent of the present invention may consist solely of the organic compound, or may contain one or more additives in addition to the organic compound as the measurement agent. Examples of additives include a pH adjuster and a stabilizer. The skin sensitization measurement reagent of the present invention may also be prepared by dissolving the measurement agent and, if necessary, the additives in water, an aqueous buffer solution, an organic solvent, or a mixture of these solvents. The reagent for measuring skin sensitization of the present invention may be provided in any form such as a solution, liquid, or solid (powder, granules, lyophilized product, tablet, etc.).

[0049] <Skin sensitization measurement method> The method for measuring skin sensitization of the present invention comprises the steps of: (1) reacting the skin sensitization measurement reagent of the present invention with a test substance; and (2) detecting the amount of the skin sensitization measurement reagent after the reaction or the amount of the reaction product by optical measurement; Includes.

[0050] The skin sensitization measurement reagent of the present invention may be used in a form dissolved in, for example, an aqueous buffer solution such as phosphate buffer or an organic solvent such as dimethyl sulfoxide (DMSO), and further diluted with an aqueous buffer solution such as phosphate buffer or another organic solvent as necessary, at a concentration of the organic compound of, for example, about 0.01 μmol / L to about 1 mol / L, usually about 1 μmol / L to about 100 μmol / L.

[0051] The type of test substance is not particularly limited, and may be, for example, at least one of fragrances, essential oils, polymer compounds, pharmaceuticals, pesticides, foods, chemical products, and plant extracts containing naturally occurring ingredients. The test substance may be dissolved in, for example, water or an organic solvent such as methanol, ethanol, acetonitrile, acetone, or DMSO (dimethyl sulfoxide), or a mixture thereof, to a concentration of, for example, about 0.01 μmol / L to about 1 mol / L, typically about 0.1 mmol / L to about 500 mmol / L. To prevent precipitation of the test substance, the test substance may be dissolved to a concentration of preferably 0.1 mmol / L to 100 mmol / L, more preferably 0.1 mmol / L to 10 mmol / L.

[0052] The organic compound, which is the main measuring agent of the skin sensitization measuring reagent of the present invention, and a test substance solution may be mixed and reacted so that the molar concentration ratio of the organic compound to the test substance is, for example, 1:200 to 10:1. The reaction can be carried out by stirring or leaving the solution containing the organic compound and the test substance to stand, usually for about 1 minute to about 2 days, while keeping the solution at a temperature in the range of, for example, about 4°C to about 60°C.

[0053] The skin sensitization potential of a test substance can be measured by examining the reactivity of the organic compound with the test substance through the above reaction. To examine the reactivity, the amount of the organic compound remaining in a mixture of the skin sensitization measurement reagent solution and the test substance solution and / or the amount of the reaction product between the organic compound and the test substance can be analyzed. By performing this analysis over time, the reaction rate constant between the organic compound and the test substance can be determined, and the reaction rate constants of different test substances can be compared. Alternatively, the skin sensitization potential of a test substance can be evaluated by comparing the reaction rate constant of the test substance with that of a compound whose skin sensitization potential and intensity have been confirmed in animal experiments.

[0054] When analyzing the residual amount, if there is a possibility that the skin sensitization measurement reagent may undergo some kind of change in the reaction solution, a reaction solution containing no test substance alone (control group) may be prepared separately and analyzed, and correction may be made based on the value of the residual amount in this reaction solution.

[0055] The method of the present invention may include subjecting the reaction product obtained in the step of reacting the skin sensitization measurement reagent with the test substance to chromatography. That is, the method for analyzing the compound and the compound produced by the reaction is not particularly limited, and the compound produced by the reaction, the organic compound, and the test substance can be separated and analyzed by, for example, high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), etc.

[0056] Chromatographic techniques that can be used for the above-mentioned HPLC, GC, or TLC include reverse phase, normal phase, ion exchange, etc. Commercially available columns and TLCs that can be used for such chromatographic techniques include, for example, LC columns such as CAPCELL-PAK (manufactured by Osaka Soda), L-column ODS (manufactured by Chemicals Evaluation and Research Institute), and Shodex Asahipak (manufactured by Showa Denko), and TLC plates such as Silica Gel 60F254 (manufactured by Merck) and Silica Gel Plate (manufactured by Nacalai Tesque, Inc.).

[0057] The method for detecting the compound produced by the reaction or the remaining organic compound is not particularly limited, and examples of detectors that can be used in the HPLC analysis include ultraviolet-visible detectors, near-infrared detectors, fluorescence detectors, differential refractive index detectors, electrical conductivity detectors, and evaporative light scattering detectors. Examples of ultraviolet-visible detectors include single-wavelength ultraviolet-visible detectors, dual-wavelength ultraviolet-visible detectors, and photodiode array detectors. Commercially available detectors that can be used in such detection methods include ultraviolet-visible detectors, differential refractive index detectors, and electrical conductivity detectors manufactured by Shimadzu Corporation, Hitachi, Waters, Shiseido, and the like, and evaporative light scattering detectors manufactured by Shimadzu Corporation, and the like.

[0058] In one example of the present invention, the reduction rate of the skin sensitization reagent (also called the nucleophilic reagent) after the reaction between the test substance and the skin sensitization reagent may be detected by optical measurement using an ultraviolet detector. Commercially available ultraviolet detectors, such as those manufactured by Shimadzu Corporation, Waters Corporation, Hitachi, Ltd., and Agilent Technologies, can be used.

[0059] In optical measurements using an ultraviolet detector, the detection wavelength is preferably 200 to 700 nm, more preferably 200 to 600 nm, more preferably 220 to 550 nm, and even more preferably 280 to 480 nm.

[0060] In another example of the present invention, the reduction rate of the skin sensitization measurement reagent (also called the nucleophilic reagent) after the reaction between the test substance and the skin sensitization measurement reagent may be detected by optical measurement using a fluorescence detector. Molecules in the ground state absorb excitation light and transition to an excited state. Part of the absorbed excitation energy is deactivated by vibrational energy, etc., and undergoes a non-radiative transition to a lower vibrational level. The light emitted when the molecule returns to the ground state is fluorescence. Optical measurements using a fluorescence detector are generally 10 times faster than absorptiometry. 3It is said to be an analytical method with more than 100 times the sensitivity. Furthermore, because it measures fluorescent substances, it has excellent selectivity and is used as an analytical method for extremely small amounts. Since the fluorescence intensity is proportional to the concentration of the fluorescent substance, quantitative analysis is possible by creating a calibration curve. Commercially available fluorescence detectors can be used, including those manufactured by Shimadzu, Waters, Hitachi, Agilent Technologies, and Osaka Soda.

[0061] In optical measurements using a fluorescence detector, the excitation wavelength is preferably 200 to 800 nm, more preferably 200 to 600 nm, even more preferably 200 to 550 nm, still more preferably 200 to 500 nm, and particularly preferably 200 to 480 nm. The fluorescence wavelength is preferably 200 to 1000 nm, more preferably 200 to 800 nm, even more preferably 200 to 700 nm, and particularly preferably 200 to 650 nm.

[0062] The reduction rate of the skin sensitization test reagent (also called the nucleophilic reagent) can be calculated from the average peak area of ​​the skin sensitization test reagent (also called the nucleophilic reagent) in optical measurement using an ultraviolet detector or a fluorescence detector according to the following formula.

[0063] Percent depletion of nucleophile = [1 - (average peak area of ​​unreacted nucleophile after reaction / average peak area of ​​standard nucleophile)] x 100

[0064] Detection in the measurement method using the skin sensitization measurement reagent of the present invention is not limited to the above, and may be performed by detecting ions of a specific mass based on molecular weight, etc., with reference to the methods described in, for example, JP 2003-14761 A or JP 2008-139275 A. The present invention will be described in detail below with reference to examples, but the present invention is not limited to these examples. [Example]

[0065] <Terminology> EDTA: Ethylenediaminetetraacetic acid TFA: Trifluoroacetic acid DMSO: dimethyl sulfoxide NMP: N-methyl-2-pyrrolidone

[0066] [ka]

[0067] [ka]

[0068] [ka]

[0069] (Synthesis of Compound 1) 269 ​​mg of 1-naphthylacetic acid (Fujifilm Wako Pure Chemical Industries, Ltd.) and 10 mL of dimethylformamide (Fujifilm Wako Pure Chemical Industries, Ltd.) were dissolved in a recovery flask, and 234 mg of 1,1'-carbonyldiimidazole (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. 500 mg of S-trityl-L-cysteine ​​(Cys(Trt)-OH) (Tokyo Chemical Industry Co., Ltd.) and 250 μL of N,N-diisopropylethylamine (Fujifilm Wako Pure Chemical Industries, Ltd.) were then added and stirred for 2 hours. After the reaction was complete, water was added to the reaction solution, which was then extracted with ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.). The organic layer was washed with water and saturated brine and then dried over anhydrous sodium sulfate (Fujifilm Wako Pure Chemical Industries, Ltd.). After filtering off the anhydrous sodium sulfate, the filtrate was evaporated in vacuo to give 690 mg of an intermediate crude product, N-(2-(naphthalen-1-yl)acetyl)-S-trityl-L-cysteine.

[0070] Next, 690 mg of the intermediate crude product and 10 mL of dimethylformamide (Fujifilm Wako Pure Chemical Industries, Ltd.) were dissolved in a recovery flask, and 230 mg of 1,1'-carbonyldiimidazole (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Next, 72 mg of hydrazine monohydrate (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Water was then added to the reaction solution, which was then extracted with ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.). The organic layer was washed with water and saturated brine and then dried over anhydrous sodium sulfate (Fujifilm Wako Pure Chemical Industries, Ltd.). The anhydrous sodium sulfate was filtered off, and the filtrate was evaporated under vacuum.

[0071] Next, 2 mL of a mixture of trifluoroacetic acid (TFA) (Fujifilm Wako Pure Chemical Industries, Ltd.): triisopropylsilane (Tokyo Chemical Industry Co., Ltd.): water (=95:2.5:2.5) was added to the distillate. After stirring for 2 hours, the solvent was distilled off under reduced pressure. The resulting residue was purified by liquid chromatography, and the solvent was distilled off under reduced pressure. The residue was then lyophilized to obtain 95 mg of a white solid (Compound 1). Observed MS(ESI m / z):304.3 (M+H), RT(min):1.03

[0072] (Synthesis of Compounds 2 to 5) It was synthesized according to the synthetic method of compound 1. Compound 2 was synthesized according to the same method as for compound 1, except that S-trityl-L-homocysteine ​​(synthesized according to the method described in the literature, Journal of Medicinal Chemistry, 1996, vol. 39, #7, p. 136) was used instead of S-trityl-L-cysteine ​​(Cys(Trt)-OH) used in the synthesis of compound 1. Compound 3 was synthesized according to the same method as for compound 1, except that S-trityl-isocysteine ​​(synthesized as described in Bioorganic and Medicinal Chemistry, 2008, vol. 16, #1, p. 65) was used instead of S-trityl-L-cysteine ​​(Cys(Trt)-OH) used in the synthesis of compound 1. Compound 4 was synthesized according to the same synthetic method as Compound 1, except that (2R)-2-(methylamino)-3-[(triphenylmethyl)sulfanyl]propanoic acid (ChemShuttle) was used instead of S-trityl-L-cysteine ​​(Cys(Trt)-OH) used in the synthesis of Compound 1. Compound 5 was synthesized according to the same synthetic method as that for compound 1, except that 4-mercaptophenylalanine (manufactured by Chemspace) was used instead of S-trityl-L-cysteine ​​(Cys(Trt)-OH) used in the synthesis of compound 1.

[0073] (Synthesis of Compound 6) Peptide solid-phase synthesis was performed using 2-chlorotrityl chloride resin (Watanabe Chemical Industry Co., Ltd.). The resin was used in an amount equivalent to 0.05 mmol. The resin was swollen with methylene chloride (Fujifilm Wako Pure Chemical Industries Co., Ltd.), and then 0.075 mmol of N-α-(9-Fluorenylmethoxycarbonyl)-L-aspartic acid β-allyl ester (Watanabe Chemical Industry Co., Ltd.) and 0.4 mL of diisopropylethylamine (Tokyo Chemical Industry Co., Ltd.) were added to a 0.5 mol / L methylene chloride solution. The mixture was shaken for 2 hours. After the reaction, the mixture was washed with methylene chloride and N-methyl-2-pyrrolidone (Fujifilm Wako Pure Chemical Industries Co., Ltd.). Next, 1-naphthylacetic acid (Fujifilm Wako Pure Chemical Industries, Ltd.) was condensed and the allyl group was deprotected, followed by condensation with 2-[(triphenylmethyl)sulfanyl]ethanamine (Combi-Blocks). After peptide synthesis, the resin was washed with dichloromethane (Fujifilm Wako Pure Chemical Industries, Ltd.) and the solvent was evaporated under reduced pressure. 2 mL of trifluoroacetic acid (TFA) (Fujifilm Wako Pure Chemical Industries, Ltd.):triisopropylsilane (Tokyo Chemical Industry Co., Ltd.):water (95:2.5:2.5) was added to cleave the peptide from the resin while simultaneously deprotecting it. After 2 hours, the resin was filtered off, and 12 mL of n-hexane (Fujifilm Wako Pure Chemical Industries, Ltd.):methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.) (1:1) was added to the filtrate to form a solid. The solid was precipitated by centrifugation, and the supernatant was removed. The solid was washed with methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was removed under reduced pressure. The intermediate crude product was then dissolved in 2 mL of dimethylformamide (Fujifilm Wako Pure Chemical Industries, Ltd.), and 32 mg (0.2 mmol) of 1,1'-carbonyldiimidazole (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Next, 25 mg (0.5 mmol) of hydrazine monohydrate (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Water was then added to the reaction solution, which was then extracted with ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.). The organic layer was washed with water and saturated brine, and then dried over anhydrous sodium sulfate (Fujifilm Wako Pure Chemical Industries, Ltd.). The anhydrous sodium sulfate was removed by filtration, and the filtrate was evaporated under vacuum.The resulting residue was purified by liquid chromatography, and the solvent was then evaporated under reduced pressure, followed by freeze-drying to obtain a white solid.

[0074] (Synthesis of Compound 7) Compound 7 was synthesized according to the same synthetic method as Compound 6, except that (R)-2-amino-3-(tritylthio)propan-1-ol (manufactured by AstaTech) was used instead of 2-[(Triphenylmethyl)sulfanyl]ethanamine used in the synthesis of Compound 6.

[0075] (Synthesis of Compound 8) Solid-phase peptide synthesis was performed using 2-chlorotrityl chloride resin (Watanabe Chemical Industry Co., Ltd.). The resin was used in an amount equivalent to 0.05 mmol. The resin was swollen with methylene chloride (Fujifilm Wako Pure Chemical Industries, Ltd.), and then 0.075 mmol of N-α-(9-Fluorenylmethoxycarbonyl)-L-aspartic acid β-allyl ester (synthesized as described in Organic Letters, 2013, Vol. 15, #19, p. 5076) and 0.4 mL of diisopropylethylamine (Tokyo Chemical Industry Co., Ltd.) were added to a 0.5 mol / L methylene chloride solution. The mixture was shaken for 2 hours. After the reaction, the mixture was washed with methylene chloride and N-methyl-2-pyrrolidone (Fujifilm Wako Pure Chemical Industries, Ltd.). Next, 1-naphthylacetic acid (Fujifilm Wako Pure Chemical Industries, Ltd.) was condensed and the allyl group was deprotected, followed by condensation of S-trityl-L-cysteine-allyl ester (synthesized by the method described in the literature, Organic Letters, 2013, vol. 15, #19, p. 5076) and deprotection of the allyl group, and then condensation of 3-aminopyridine (Fujifilm Wako Pure Chemical Industries, Ltd.) was carried out.

[0076] After peptide synthesis, the resin was washed with dichloromethane (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. Two mL of a mixture of trifluoroacetic acid (TFA) (Fujifilm Wako Pure Chemical Industries, Ltd.):triisopropylsilane (Tokyo Chemical Industry Co., Ltd.):water (95:2.5:2.5) was added to cleave the peptide from the resin while simultaneously deprotecting it. After 2 hours, the resin was filtered off, and 12 mL of a mixture of n-hexane (Fujifilm Wako Pure Chemical Industries, Ltd.):methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.) (1:1) was added to the filtrate to form a solid. The solid was precipitated by centrifugation, and the supernatant was removed. The solid was washed with methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. Next, the intermediate crude product was dissolved in 2 mL of dimethylformamide (Fujifilm Wako Pure Chemical Industries, Ltd.), and 32 mg (0.2 mmol) of 1,1'-carbonyldiimidazole (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Next, 25 mg (0.5 mmol) of hydrazine monohydrate (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Water was then added to the reaction solution, which was then extracted with ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.). The organic layer was washed with water and saturated brine and then dried over anhydrous sodium sulfate (Fujifilm Wako Pure Chemical Industries, Ltd.). The anhydrous sodium sulfate was removed by filtration, and the filtrate was evaporated under vacuum. The resulting residue was purified by liquid chromatography, the solvent was evaporated under reduced pressure, and the residue was lyophilized to obtain a white solid.

[0077] (Synthesis of Compound 9) Compound 9 was synthesized according to the same synthetic method as that for compound 8, except that cyclopropylamine (Tokyo Chemical Industry Co., Ltd.) was used instead of 3-aminopyridine used in the synthesis of compound 8.

[0078] (Synthesis of Compound 10) Solid-phase peptide synthesis was performed using 2-chlorotrityl chloride resin (Watanabe Chemical Industry Co., Ltd.). The resin was used in an amount equivalent to 0.05 mmol. The resin was swollen with methylene chloride (Fujifilm Wako Pure Chemical Industries, Ltd.), and then 0.075 mmol of N-α-Fmoc-N-β-alloc-L-diaminopropionic acid (Iris Biotech) and 0.4 mL of diisopropylethylamine (Tokyo Chemical Industry Co., Ltd.) were added to a 0.5 mol / L methylene chloride solution. The mixture was then shaken for 2 hours. After the reaction, the mixture was washed with methylene chloride and N-methyl-2-pyrrolidone (Fujifilm Wako Pure Chemical Industries, Ltd.). Next, 1-naphthylacetic acid (Fujifilm Wako Pure Chemical Industries, Ltd.) was condensed and the allyl group was deprotected, followed by N-α-(9-Fluorenylmethoxycarbonyl)-S-trityl-L-cysteine ​​(Watanabe Chemical Industry Co., Ltd.). Next, the Fmoc group was deprotected, followed by condensation with 2-thiophenecarboxylic acid (Fujifilm Wako Pure Chemical Industries, Ltd.) and washing with N-methyl-2-pyrrolidone.

[0079] After peptide synthesis, the resin was washed with dichloromethane (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. Two mL of a mixture of trifluoroacetic acid (TFA) (Fujifilm Wako Pure Chemical Industries, Ltd.):triisopropylsilane (Tokyo Chemical Industry Co., Ltd.):water (95:2.5:2.5) was added to cleave the peptide from the resin while simultaneously deprotecting it. After 2 hours, the resin was filtered off, and 12 mL of a mixture of n-hexane (Fujifilm Wako Pure Chemical Industries, Ltd.):methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.) (1:1) was added to the filtrate to form a solid. The solid was precipitated by centrifugation, and the supernatant was removed. The solid was washed with methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. Next, the intermediate crude product was dissolved in 2 mL of dimethylformamide (Fujifilm Wako Pure Chemical Industries, Ltd.), and 32 mg (0.2 mmol) of 1,1'-carbonyldiimidazole (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Next, 25 mg (0.5 mmol) of hydrazine monohydrate (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Water was then added to the reaction solution, which was then extracted with ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.). The organic layer was washed with water and saturated brine and then dried over anhydrous sodium sulfate (Fujifilm Wako Pure Chemical Industries, Ltd.). The anhydrous sodium sulfate was removed by filtration, and the filtrate was evaporated under vacuum. The resulting residue was purified by liquid chromatography, the solvent was evaporated under reduced pressure, and the residue was lyophilized to obtain a white solid.

[0080] (Synthesis of Compound 11) Solid-phase peptide synthesis was performed using 2-chlorotrityl chloride resin (Watanabe Chemical Industry Co., Ltd.). The resin was used in an amount equivalent to 0.05 mmol. The resin was swollen with methylene chloride (Fujifilm Wako Pure Chemical Industries, Ltd.), and then 0.075 mmol of (9H-fluoren-9-yl)methyl N-(2-sulfanylethyl)carbamate (synthesized as described in Tetrahedron Letters, 2005, Vol. 46, #43, p. 7443) and 0.4 mL of diisopropylethylamine (Tokyo Chemical Industry Co., Ltd.) were added to a 0.5 mol / L methylene chloride solution. The mixture was shaken for 2 hours. After the reaction, the mixture was washed with methylene chloride and N-methyl-2-pyrrolidone (Fujifilm Wako Pure Chemical Industries, Ltd.). Next, N-α-(9-Fluorenylmethoxycarbonyl)-L-aspartic acid β-allyl ester (Watanabe Chemical Industry Co., Ltd.) was condensed with 1-naphthylacetic acid (Fujifilm Wako Pure Chemical Industries, Ltd.). After deprotection of the allyl group, hydrazine monohydrate (Fujifilm Wako Pure Chemical Industries, Ltd.) was added.

[0081] After peptide synthesis, the resin was washed with dichloromethane (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. 2 mL of a mixture of trifluoroacetic acid (TFA) (Fujifilm Wako Pure Chemical Industries, Ltd.):triisopropylsilane (Tokyo Chemical Industry Co., Ltd.):water (95:2.5:2.5) was added to cleave the peptide from the resin while simultaneously deprotecting it. After 2 hours, the resin was filtered off, and 12 mL of a mixture of n-hexane (Fujifilm Wako Pure Chemical Industries, Ltd.):methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.) (1:1) was added to the filtrate to produce a solid. The solid was precipitated by centrifugation, and the supernatant was removed. The solid was washed with methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. The resulting residue was purified by liquid chromatography, the solvent was evaporated under reduced pressure, and the residue was lyophilized to yield a white solid.

[0082] (Synthesis of Compound 12) Compound 12 was synthesized according to the same synthetic method as compound 11, except that (9H-fluoren-9-yl)methyl (R)-2-(mercaptomethyl)pyrrolidine-1-carboxylate (synthesized as described in the literature, Synlett, 2010, #7, p. 1037) was used instead of (9H-fluoren-9-yl)methyl N-(2-sulfanylethyl)carbamate used in the synthesis of compound 11.

[0083] (Synthesis of Compound 13) 120 mg (0.6 mmol) of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Tokyo Chemical Industry Co., Ltd.) and 10 mL of dimethylformamide (Fujifilm Wako Pure Chemical Industries, Ltd.) were dissolved in a recovery flask, and 200 mg (0.55 mmol) of S-trityl-L-cysteine ​​(Cys(Trt)-OH) (Tokyo Chemical Industry Co., Ltd.) and 250 μL of N,N-diisopropylethylamine (Fujifilm Wako Pure Chemical Industries, Ltd.) were added and stirred for 2 hours. After the reaction was completed, water was added to the reaction solution, which was then extracted with ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.). The organic layer was washed with water and saturated brine and then dried over anhydrous sodium sulfate (Fujifilm Wako Pure Chemical Industries, Ltd.).

[0084] Next, the entire intermediate crude product and 5 mL of dimethylformamide (Fujifilm Wako Pure Chemical Industries, Ltd.) were placed in a recovery flask and dissolved. 113 mg (0.7 mmol) of 1,1'-carbonyldiimidazole (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Next, 50 mg (1.0 mmol) of hydrazine monohydrate (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Water was then added to the reaction solution, which was then extracted with ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.). The organic layer was washed with water and saturated brine and then dried over anhydrous sodium sulfate (Fujifilm Wako Pure Chemical Industries, Ltd.). The anhydrous sodium sulfate was removed by filtration, and the filtrate was evaporated under vacuum.

[0085] Next, 2 mL of a mixture of trifluoroacetic acid (TFA) (Fujifilm Wako Pure Chemical Industries, Ltd.): triisopropylsilane (Tokyo Chemical Industry Co., Ltd.): water (=95:2.5:2.5) was added to the distillate. After stirring for 2 hours, the solvent was distilled off under reduced pressure. The resulting residue was purified by liquid chromatography, and the solvent was distilled off under reduced pressure. The residue was then freeze-dried to obtain a white solid.

[0086] (Synthesis of Compound 14) Peptide solid-phase synthesis was performed using 2-chlorotrityl chloride resin (Watanabe Chemical Industry Co., Ltd.). The resin was used in an amount equivalent to 0.05 mmol. The resin was swollen with methylene chloride (Fujifilm Wako Pure Chemical Industries Co., Ltd.), and then 0.075 mmol of N-α-(9-Fluorenylmethoxycarbonyl)-L-aspartic acid β-allyl ester (Watanabe Chemical Industry Co., Ltd.) and 0.4 mL of diisopropylethylamine (Tokyo Chemical Industry Co., Ltd.) were added to a 0.5 mol / L methylene chloride solution. The mixture was shaken for 2 hours. After the reaction, the mixture was washed with methylene chloride and N-methyl-2-pyrrolidone (Fujifilm Wako Pure Chemical Industries Co., Ltd.). Next, 2-[(Triphenylmethyl)sulfanyl]ethanamine (Combi-Blocks) was condensed and the allyl group was deprotected, followed by mono-Fmoc ethylene diamine hydrochloride condensation. After the Fmoc group was deprotected, 2 mL of N-methyl-2-pyrrolidone solution of 12 mg (0.06 mmol) of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Tokyo Chemical Industry Co., Ltd.) was added, shaken for 1 hour, and washed with N-methyl-2-pyrrolidone.

[0087] After peptide synthesis, the resin was washed with dichloromethane (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. Two mL of a mixture of trifluoroacetic acid (TFA) (Fujifilm Wako Pure Chemical Industries, Ltd.):triisopropylsilane (Tokyo Chemical Industry Co., Ltd.):water (95:2.5:2.5) was added to cleave the peptide from the resin while simultaneously deprotecting it. After 2 hours, the resin was filtered off, and 12 mL of a mixture of n-hexane (Fujifilm Wako Pure Chemical Industries, Ltd.):methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.) (1:1) was added to the filtrate to form a solid. The solid was precipitated by centrifugation, and the supernatant was removed. The solid was washed with methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. Next, the intermediate crude product was dissolved in 2 mL of dimethylformamide (Fujifilm Wako Pure Chemical Industries, Ltd.), and 32 mg (0.2 mmol) of 1,1'-carbonyldiimidazole (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Next, 25 mg (0.5 mmol) of hydrazine monohydrate (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 2 hours. Water was then added to the reaction solution, which was then extracted with ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.). The organic layer was washed with water and saturated brine and then dried over anhydrous sodium sulfate (Fujifilm Wako Pure Chemical Industries, Ltd.). The anhydrous sodium sulfate was removed by filtration, and the filtrate was evaporated under vacuum. The resulting residue was purified by liquid chromatography, the solvent was evaporated under reduced pressure, and the residue was lyophilized to obtain a white solid.

[0088] (Synthesis of Compound 15) 300 mg of 2,6-Naphthalendiacetic acid (A1 Biochem Lab), 295 mg of N-hydroxysuccinimide (Tokyo Chemical Industry Co., Ltd.), and 10 mL of dimethylformamide (Fujifilm Wako Pure Chemical Industries, Ltd.) were placed in a recovery flask. Next, 671 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (Dojindo Laboratories, Inc.) was added and stirred for 2 hours. After the reaction was completed, water was added to the reaction solution, which was then extracted with ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.). The organic layer was washed with water and saturated brine and then dried over anhydrous sodium sulfate (Fujifilm Wako Pure Chemical Industries, Ltd.). The anhydrous sodium sulfate was filtered off, and the filtrate was evaporated under vacuum. The residue was then purified by silica gel chromatography (n-hexane (Fujifilm Wako Pure Chemical Industries, Ltd.): ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.) = 1:0 to 0.7:0.3) to obtain 480 mg of a white solid, bis(1,5-dioxopyrrolidin-1-yl) 2,2'-(naphthalene-2,6-diyl) diacetate. Observed MS(ESI m / z):439.2 (M+H), RT(min):1.21

[0089] Next, 56 mg (0.13 mmol) of bis(1,5-dioxopyrrolidin-1-yl) 2,2'-(naphthalene-2,6-diyl) diacetate, 23 mg (0.06 mmol) of S-trityl-L-cysteinamide (Combi-Blocks), and 1 mL of dimethylformamide (Fujifilm Wako Pure Chemical Industries, Ltd.) were placed in a recovery flask and immersed in a 65 °C oil bath and stirred for 3 hours. The reaction solution was then cooled to room temperature, and 25 mg (0.5 mmol) of hydrazine monohydrate (Fujifilm Wako Pure Chemical Industries, Ltd.) was added and stirred for 1 hour. After completion of the reaction, water was added to the reaction solution, which was then extracted with ethyl acetate (Fujifilm Wako Pure Chemical Industries, Ltd.). The organic layer was washed with water and saturated brine and then dried over anhydrous sodium sulfate (Fujifilm Wako Pure Chemical Industries, Ltd.). The anhydrous sodium sulfate was filtered off, and the filtrate was evaporated under vacuum. Next, 1 mL of trifluoroacetic acid (TFA) (Fujifilm Wako Pure Chemical Industries, Ltd.):triisopropylsilane (Tokyo Chemical Industry Co., Ltd.):water (=95:2.5:2.5) was added, and after 2 hours, 6 mL of n-hexane (Fujifilm Wako Pure Chemical Industries, Ltd.):methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.) (=1:1) was added to produce a solid. The solid was precipitated by centrifugation, and the supernatant was removed. The solid was washed with methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. The resulting residue was purified by liquid chromatography, the solvent was evaporated under reduced pressure, and the residue was lyophilized to obtain a white solid.

[0090] (Synthesis of Comparative Example 1) Solid-phase peptide synthesis was carried out using Rink Amide-ChemMatrix (Biotage) (0.45 mmol / g) as the resin for solid-phase synthesis. 111.1 mg (0.05 mmol) of resin was used. Condensation was carried out in this order using (S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-S-trityl-L-cysteine ​​(Fmoc-Cys(Trt)-OH) (Watanabe Chemical Co., Ltd.) and 1-naphthylacetic acid (Fujifilm Wako Pure Chemical Industries, Ltd.). After elongation, the resin was washed with dichloromethane (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. 2 mL of a mixture of trifluoroacetic acid (TFA) (Fujifilm Wako Pure Chemical Industries, Ltd.):triisopropylsilane (Tokyo Chemical Industry Co., Ltd.):water (=95:2.5:2.5) was added, and the peptide was cleaved from the resin while simultaneously being deprotected. After 2 hours, the resin was filtered off, and 12 mL of n-hexane (Fujifilm Wako Pure Chemical Industries, Ltd.):methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.) (1:1) was added to the filtrate to produce a solid. The solid was precipitated by centrifugation, and the supernatant was removed. The solid was washed with methyl t-butyl ether (Fujifilm Wako Pure Chemical Industries, Ltd.), and the solvent was evaporated under reduced pressure. The resulting residue was purified by liquid chromatography, and the solvent was evaporated under reduced pressure. The residue was then lyophilized to yield 6.4 mg of a white solid. Observed MS(ESI m / z):289.2(M+H), RT(min):1.09

[0091] Solid-phase peptide synthesis using an automated peptide synthesizer Solid-phase peptide synthesis was performed using an automated peptide synthesizer (Biotage SyroI). The synthesizer was loaded with a solid-phase synthesis resin, 4 equivalents of Fmoc amino acid (0.5 mol / L) in N-methyl-2-pyrrolidone (NMP), 4 equivalents of cyanohydroxyiminoacetic acid ethyl ester (1 mol / L) in NMP, 4 equivalents of diisopropylcarbodiimide (1 mol / L) in NMP, and piperidine (20% v / v) in NMP. One cycle consisted of Fmoc deprotection (20 min), washing with NMP, Fmoc amino acid condensation (1 h), and washing with NMP. The peptide chain was elongated by repeating this cycle.

[0092] For deprotection of the allyl group, 58 mg (0.05 mmol) of tetrakis(triphenylphosphine)palladium(0) (Tokyo Chemical Industry Co., Ltd.), 1.85 mL of chloroform (Fujifilm Wako Pure Chemical Industries, Ltd.), 0.1 mL of acetic acid (Fujifilm Wako Pure Chemical Industries, Ltd.), and 0.05 mL of N-methylmorpholine (Fujifilm Wako Pure Chemical Industries, Ltd.) were added and shaken for 2 hours. After the reaction was completed, the mixture was washed with NMP.

[0093] The crude product obtained was purified by liquid chromatography. Column: Waters X Select CSH Prep C18 5 μm OBD (19 x 250 mm) Column temperature: 40 degrees Flow rate: 20ml / min Detection wavelength: 220nm, 254nm Solvent: Solution A: 0.1% formic acid-water Solution B: 0.1% formic acid-acetonitrile Fmoc amino acids were obtained from Watanabe Chemical Industry Co., Ltd. N-methyl-2-pyrrolidone, diisopropylethylamine, diisopropylcarbodiimide, piperidine, and acetic anhydride were obtained from Fujifilm Wako Pure Chemical Industries, Ltd. Cyano-hydroxyimino-acetic acid ethyl ester was obtained from Tokyo Chemical Industry Co., Ltd.

[0094] Mass spectra (MS) were measured using an ACQUITY SQD LC / MS System (Waters, ionization method: ESI (ElectroSpray Ionization)). Retention times (RT) were measured using an ACQUITY SQD LC / MS System (Waters) and are shown in minutes (min). Column: Waters BEHC 18 1.7 μm, 2.1 x 30 mm Solvent: Solution A: 0.1% formic acid-water Solution B: 0.1% formic acid-acetonitrile Gradient cycle: 0.00 min (solution A / solution B = 95 / 5), 2.00 min (solution A / solution B = 5 / 95), 3.00 min (solution A / solution B = 95 / 5) Flow rate: 0.5mL / min Column temperature: room temperature Detection wavelength: 254 nm

[0095] (Synthesis of Comparative Example 2) The synthesis was carried out with reference to Examples 1 to 5 of JP 2009-222466 A.

[0096] <Test Method> (1) Preparation of various solutions (1-1)0.1mmol / L EDTA aqueous solution 1) Weigh out 37.2 mg of EDTA·2Na·2H2O (Dojindo Laboratories) into a 15 mL conical tube, and add 10 mL of distilled water (Hikari Pharmaceutical, Japanese Pharmacopoeia Water for Injection) using a 25 mL measuring pipette to dissolve (10 mmol / L EDTA aqueous solution). 2) Add 49.5 mL of distilled water (Hikari Pharmaceutical, Japanese Pharmacopoeia Water for Injection) to a 100 mL container using a 50 mL measuring pipette, add 0.5 mL of the 10 mmol / L EDTA aqueous solution from 1) above, mix, and dilute 100 times (0.1 mmol / L EDTA aqueous solution).

[0097] (1-2) 100 mmol / L phosphate buffer (pH 7.4 and pH 8.0) 1) Weigh 0.6 g of anhydrous sodium dihydrogen phosphate (special grade, manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) into a 100 mL container, and add 50 mL of distilled water (Japanese Pharmacopoeia Water for Injection, manufactured by Hikari Pharmaceutical Co., Ltd.) using a 50 mL measuring pipette to dissolve. 2) Add 300 mL of distilled water (Hikari Pharmaceutical, Japanese Pharmacopoeia Water for Injection) to a 500 mL container using a 50 mL (or 100 mL) measuring pipette. 3) Weigh out 4.26 g of anhydrous disodium hydrogen phosphate (special grade, manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) and add it to the distilled water (Japanese Pharmacopoeia Water for Injection, manufactured by Hikari Pharmaceutical Co., Ltd.) from 2) and dissolve it. 4) While measuring the pH with a pH meter, add an appropriate amount of the anhydrous sodium dihydrogen phosphate solution from 1) to the anhydrous disodium hydrogen phosphate solution from 3) using a 25 mL measuring pipette to adjust the pH to 7.4 or 8.0. 5) Using a 50 mL measuring pipette, transfer 299 mL of the solution from 4) to a new 500 mL container and add 1 mL of 0.1 mmol / L EDTA aqueous solution to make 300 mL. The concentration of EDTA in this solution is 0.33 μmol / L, and the concentration in the reaction solution is 0.25 μmol / L. 6) Sterilize the above solution by filtration through a 0.22 μm filter.

[0098] (1-3) Reaction stop solution 1) Reaction stop solution for UV detection (2.5% (v / v) TFA aqueous solution) Add 2.5 mL of TFA (Fujifilm Wako Pure Chemical Industries, special grade) to 100 mL of distilled water (Fujifilm Wako Pure Chemical Industries, Ltd.). 2) Fluorescence detection reaction stop solution (0.5% (v / v) TFA aqueous solution) Add 0.5 mL of TFA (Fujifilm Wako Pure Chemical Industries, special grade) to 100 mL of distilled water (Fujifilm Wako Pure Chemical Industries, Ltd.).

[0099] (1-4) HPLC mobile phase A: 0.1% (v / v) TFA aqueous solution Add 1.0 mL of TFA to 1 L of distilled water (Fujifilm Wako Pure Chemical Industries, Ltd.).

[0100] (1-5) HPLC mobile phase B: 0.1% (v / v) TFA in acetonitrile Add 1.0 mL of TFA to 1 L of HPLC-grade acetonitrile (Fujifilm Wako Pure Chemical Industries, Ltd., for HPLC).

[0101] (2) Preparation of nucleophilic reagent stock solution The same stock solution is used for each test, and is stored in aliquots that can be used up for each test. A specific preparation example is shown below. 1) Nucleophilic reagents are dissolved in DMSO (Fujifilm Wako Pure Chemical Industries, Ltd.) according to their molecular weights to prepare 2 mmol / L nucleophilic reagent solutions. 2) Add 149.5 mL of the same buffer solution to a 500 mL container using a 50 mL measuring pipette, add 0.5 mL of the 2 mmol / L nucleophile solution described above, and mix by inversion to dilute 300 times (6.667 μmol / L). Store this solution frozen at -70°C or below.

[0102] (3) Preparation of test substance solution Select one solvent capable of preparing a 1 mmol / L test substance solution from the following priority order: water, acetonitrile, acetone, or 5% DMSO acetonitrile solution. If water, acetonitrile, or acetone is selected, first prepare a 20 mmol / L test substance solution. To an appropriate amount of test substance, add solvent to make a 20 mmol / L solution and dissolve completely. Then, take a portion of the 20 mmol / L solution and dilute it 20-fold with the same solvent to prepare a 1 mmol / L test substance solution. If 5% DMSO acetonitrile solution is selected, prepare a 20 mmol / L DMSO solution as described above. Then, take a portion of this solution and dilute it 20-fold with acetonitrile to prepare a 1 mmol / L test substance solution.

[0103] (4) Reaction (4-1) Addition The test substance solution is prepared in a 96-well plate (U96 PP-0.5 ML NATURAL, Thermo (NUNC)) mainly using a 12-channel pipette, and the reagent is added according to the following doses. Nucleophile: 150 μL Test substance solution: 50 μL

[0104] (4-2) Reaction Plate seal (TORAST TM The plate is tightly sealed with a 96-well Seal E Type (Shimadzu GLC) and agitated using a plate shaker (Titramax 100, Heidolph). After spinning down in a centrifuge, the plate is incubated at 25°C for 24 hours in the dark.

[0105] (4-3) Stopping the reaction After 24 hours of incubation, remove the plate seal. If UV detection is used for HPLC measurement (described below), add 50 μL of UV detection reaction stop solution (2.5% (v / v) TFA aqueous solution) to each sample to stop the reaction. If fluorescence detection is used, dispense 180 μL of fluorescence detection reaction stop solution (0.5% (v / v) TFA aqueous solution) into a new plate and add 20 μL of the incubated reaction mixture to stop the reaction.

[0106] (5) HPLC measurement The HPLC measurement conditions for the nucleophilic reagent are shown below. Note that the elution conditions were selected from Condition 1, Condition 2, and Condition 3 depending on the nucleophilic reagent.

[0107] [Table 1] JPEG0007750853000027.jpg133170

[0108] (6) Data analysis (6-1) Calculation of the reduction rate From the average value of the peak area of ​​the nucleophilic reagent, the reduction rate of the nucleophilic reagent is calculated according to the following formula. Percent depletion of nucleophile (%) = [1 - (peak area of ​​unreacted nucleophile after reaction / average peak area of ​​standard nucleophile)] x 100

[0109] (7) Evaluation items (7-1) Stability of nucleophiles (especially the degree of oxidation of cysteine) The reaction mixture is measured by HPLC-UV immediately after preparation (0 hours) and after 24 hours of incubation at 25°C (24 hours). At this time, the nucleophilic reagent and its oxidized and modified forms can be confirmed by HPLC, so the remaining rate of the nucleophilic reagent is calculated based on the following formula.

[0110] Residual rate of nucleophilic reagent (%) = area value of nucleophilic reagent / (area value of nucleophilic reagent + area value of oxidized form of nucleophilic reagent + area value of transformed form of nucleophilic reagent) x 100

[0111] (7-2) Fluorescence detection sensitivity of nucleophiles Fluorescence detection is performed by determining the fluorescence of the naphthalene ring (peak area detected at an excitation wavelength of 284 nm and a fluorescence wavelength of 333 nm).

[0112] (7-3) Evaluation of reactivity with sensitizing substances The 15 substances shown in the table below were used in the reactivity evaluation, and were selected to include substances for which it is difficult to distinguish between sensitizing and non-sensitizing properties using the prior art DPRA and ADRA.

[0113] [Table 2]

[0114] The reactivity was compared with the depletion of cysteine ​​and lysine peptides in the prior art DPRA, the depletion of NAC and NAL in ADRA, NAC with an amide N-terminus (NAC-amide), and the depletion of the reagent (GSH-NBD) described in JP 2009-222466 A.

[0115] Example 1 One nucleophilic reagent (compound 1) was evaluated. The following compounds were also evaluated in the same manner as controls for comparison of reactivity with the 15 evaluation substances. Cys peptide: Lys peptide [ka]

[0116] NAC: N-[2-(naphthalen-1-yl)acetyl]cysteine NAL: α-N-[2-(naphthalen-1-yl)acetyl]lysine NAC-amide: (R)-3-mercapto-2-(2-(naphthalen-1-yl)acetamido)propanamide GSH-NBD: the compound described in paragraph 0043 of JP 2009-222466 A

[0117] (Test substance and solution preparation) For the 15 substances listed in "(7-3) Evaluation of reactivity with sensitizing substances," 1 mmol / L solutions were prepared and used in the test. For compound 1, a stock solution prepared using a pH 7.4 or pH 8.0 buffer solution when preparing the 6.667 μmol / L solution in "(2) Preparation of nucleophilic reagent stock solution" above was used. For NAC-amide and GSH-NBD, only stock solutions prepared using a pH 8.0 buffer solution were used.

[0118] (Measurement conditions) Nucleophilic reagent depletion (%) was determined under the HPLC measurement conditions described above in "(5) HPLC Measurement." However, for GSH-NBD, HPLC detection was performed using UV detection at a detection wavelength of 338 nm.

[0119] (result) (1) Stability of nucleophilic reagents The residual rate of Compound 1 was calculated immediately after the solution preparation (0 hours) and after 24 hours. The results are shown in Figure 1. More than 90% of Compound 1 remained at 0 hours, and more than 85% remained even after 24 hours, showing no significant decrease in the residual rate.

[0120] (2) Fluorescence detection sensitivity of nucleophilic reagents The fluorescence intensity (peak area in HPLC) of a stock solution of a nucleophilic reagent prepared using a pH 8.0 buffer solution was measured immediately after preparation (0 hours). The results are shown in Figure 2. A peak area sufficient for quantifying the nucleophilic reagent was detected.

[0121] (3) Reactivity of nucleophiles The reactivity of Compound 1 with 15 types of evaluation substances was calculated. Figure 3 shows the results of a comparison with the depletion of each nucleophilic reagent for substances No. 1 to No. 8 listed in Table 2 of "(7-3) Evaluation of reactivity with sensitizing substances," and Figure 4 shows similar results for substances No. 9 to No. 15.

[0122] The results showed that compound 1 showed higher reactivity than cysteine ​​peptides and lysine peptides in the DPRA with five sensitizers: nonanoyl chloride, methyl pyruvate, 10-undecenal, α-pentylcinnamaldehyde, and cyclamen aldehyde. In contrast, compound 1 showed lower reactivity than cysteine ​​peptides or lysine peptides with four sensitizers: diethyl sulfate, 3-propylidenephthalide, tropolone, and phenyl benzoate. However, all of these showed depletion rates of 5% or higher, confirming some degree of reactivity. Furthermore, compound 1 showed no reactivity with 1-bromobutane and 1-iodohexane, which are nonsensitizers but show reactivity with cysteine ​​peptides in the DPRA. This result is consistent with the actual sensitization information (non-sensitizing). The other four substances (diphenylcyclopropenone, trimellitic anhydride, 4'-methoxyacetophenone, and ethyl benzoylacetate) showed reactivity comparable to that of cysteine ​​peptides or lysine peptides.

[0123] Next, when compared with NAC and NAL in ADRA, the reactivity of Compound 1 was higher with nine sensitizing substances: diphenylcyclopropenone, nonanoyl chloride, methyl pyruvate, diethyl sulfate, tropolone, 10-undecenal, α-pentylcinnamaldehyde, phenyl benzoate, and cyclamen aldehyde. On the other hand, Compound 1 showed higher reactivity than trimellitic anhydride, but the depletion of Compound 1 was 41.0% (pH 7.4) and 37.2% (pH 8.0), confirming sufficient reactivity. The other five substances (3-propylidenephthalide, 1-bromobutane, 1-iodohexane, 4'-methoxyacetophenone, and ethyl benzoylacetate) showed reactivity comparable to that of NAC or NAL.

[0124] Compared to NAC-amide, compound 1 showed higher reactivity with five sensitizing substances: trimellitic anhydride, methyl pyruvate, 10-undecenal, α-pentylcinnamaldehyde, and cyclamen aldehyde. It showed similar reactivity to NAC-amide with the other 10 substances (diphenylcyclopropenone, nonanoyl chloride, diethyl sulfate, 3-propylidenephthalide, tropolone, phenyl benzoate, 1-bromobutane, 1-iodohexane, 4'-methoxyacetophenone, and ethyl benzoylacetate).

[0125] Compared with GSH-NBD, compound 1 showed higher reactivity with five sensitizing substances: methyl pyruvate, diethyl sulfate, 10-undecenal, α-pentylcinnamaldehyde, and cyclamen aldehyde. In contrast, GSH-NBD showed higher reactivity with trimellitic anhydride and nonanoyl chloride, but compound 1 showed sufficient reactivity, with depletion rates of 35% or higher for all substances. Compound 1 and GSH-NBD showed comparable reactivity with the remaining eight substances (diphenylcyclopropenone, 3-propylidenephthalide, tropolone, phenyl benzoate, 1-bromobutane, 1-iodohexane, 4'-methoxyacetophenone, and ethyl benzoylacetate).

[0126] The reactivity (depletion) of compound 1, NAC-amide, and GSH-NBD to 15 types of evaluation substances was predicted for skin sensitization using the 5.6% depletion criterion, which is the criterion for the prior art ADRA method of predicting skin sensitization using NAC alone. Table 3 shows a comparison of the results for compound 1 with the prediction results for NAC-amide and GSH-NBD performed above and the prediction results for DPRA and ADRA published in the literature.

[0127] [Table 3]

[0128] As a result of the above, four sensitizers (nonanoyl chloride, methyl pyruvate, 10-undecenal, and α-pentylcinnamaldehyde) that were incorrectly judged as negative by the DPRA were correctly judged as positive by Compound 1. Similarly, eight sensitizers (methyl pyruvate, diethyl sulfate, 3-propylidenephthalide, tropolone, 10-undecenal, α-pentylcinnamaldehyde, phenyl benzoate, and cyclamen aldehyde) that were incorrectly judged as negative by the ADRA were correctly judged as positive by Compound 1. For NAC-amide, five sensitizers (trimellitic anhydride, methyl pyruvate, 3-propylidenephthalide, 10-undecenal, and α-pentylcinnamaldehyde) that were incorrectly judged as negative were correctly judged as positive by Compound 1. For GSH-NBD, four sensitizing substances (methyl pyruvate, diethyl sulfate, 3-propylidenephthalide, and α-pentylcinnamaldehyde) that were incorrectly judged as negative were correctly judged as positive by Compound 1. In addition, two non-sensitizing substances (1-bromobutane and 1-iodohexane) that were incorrectly judged as positive by DPRA were correctly judged as negative by Compound 1.

[0129] From the above, it is believed that Compound 1 may be able to predict sensitizers with higher sensitivity than the prior art DPRA and ADRA, and that it is highly likely to be able to correctly evaluate sensitizers that were difficult to predict using conventional skin sensitization measurement methods.

Claims

1. A skin sensitization measurement reagent containing, as a measurement agent, an organic compound having a mercapto group and a hydrazide structure and having an absorption spectrum in the ultraviolet, visible light, or near-infrared region, wherein the organic compound is represented by the following formula (6): 【Chemical 1】 During the ceremony, R ６ represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these groups do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. ６１ -CO-, -CO-NJ ６１ - or -NH-CO-NH-, J ６１ represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X ６ represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these may be substituted with -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- in the molecular chain. ６２ -CO-, -CO-NJ ６２ - or -NH-CO-NH-, J ６２ represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. Z ６ is -CO-NR ６１ NR ６２ R ６３ indicates R ６１ , R ６２ , and R ６３ are each independently a hydrogen atom or an alkyl group having 1 to 10 carbon atoms. n represents 0 or 1, m represents 0 or 1.

2. A skin sensitization measurement reagent comprising, as a measurement agent, an organic compound having a mercapto group and a hydrazide structure and having an absorption spectrum in the ultraviolet, visible light or near-infrared region, wherein the organic compound is represented by the following formula (7): 【Chemistry 2】 During the ceremony, R ７ represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, or a cycloalkyl group having 3 to 10 carbon atoms, and these groups do not contain -O-, -C(O)-, -OC(O)-, -NJ- in the molecular chain. ７１ -CO-, -CO-NJ ７１ - or -NH-CO-NH-, J ７１ represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a mercapto group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. X ７ represents an alkyl group having 1 to 10 carbon atoms and having one or more mercapto groups, a cycloalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, an arylalkyl group having 7 to 12 carbon atoms and having one or more mercapto groups, a heteroalkylalkyl group having 3 to 10 carbon atoms and having one or more mercapto groups, or a mercapto group, and these may be substituted with -O-, -C(O)-, -OC(O)-, -NJ- or -NJ- in the molecular chain. ７２ -CO-, -CO-NJ ７２ - or -NH-CO-NH-, J ７２ represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, and the alkyl group or cycloalkyl group may have a substituent selected from a cycloalkyl group having 3 to 6 carbon atoms, an amino group, a cyano group, a hydroxyl group, a carboxy group, a phenyl group, a hydroxyphenyl group, a pyridyl group, a naphthyl group, a thienyl group, or a furyl group. W is NR ７１ -NR ７２ R ７３ indicates R ７１ , R ７２ , and R ７３ represents a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, n represents 0 or 1.

3. (1) reacting the skin sensitization measurement reagent according to claim 1 or 2 with a test substance; and (2) detecting the amount of the skin sensitization measurement reagent after the reaction or the amount of the reaction product by optical measurement; A method for measuring skin sensitization, including:

4. 4. The method for measuring skin sensitization according to claim 3, wherein the test substance is at least one of a fragrance, an essential oil, a polymer compound, a pharmaceutical, an agricultural chemical, a food, a chemical product, and a plant extract containing a component derived from a natural product.

5. The method for measuring skin sensitization according to claim 3 or 4, further comprising subjecting a reaction product obtained in the step of reacting the skin sensitization measurement reagent with the test substance to chromatography.

6. The method for measuring skin sensitization according to any one of claims 3 to 5, wherein the optical measurement is a measurement using a fluorescence detector, and the excitation wavelength is 200 to 600 nm and the fluorescence wavelength is 200 to 800 nm.

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