topical skin preparations

Abstract

To provide an active ingredient which is derived from a natural product, has excellent biological safety, and promotes vitamin C uptake into cells. [Solution] A vitamin C uptake promoter containing as an active ingredient one or more of the following: a fermentation product of a plant of the genus Nelumbo in the family Nelumbo or an extract thereof with lactic acid bacteria, yeast, koji mold or Bacillus subtilis; a hydrolyzed plant of the genus Oryza in the family Poaceae or an extract thereof; an extract of a plant of the genus Salicornia in the family Chenopodiaceae; and a hydrolyzed soybean or an extract thereof, as well as a topical skin preparation or oral composition containing these agents.

Description

[Technical Field]

[0001] The present invention relates to a novel active ingredient derived from a natural product, which has excellent physiological activity and biological safety, and to an external preparation for the skin (including the scalp) and an oral composition for beauty or health promotion containing the same. [Background technology]

[0002] In recent years, research has been conducted on cell aging and cell damage caused by external factors (e.g., ultraviolet rays, chemical substances such as air pollutants and environmental hormones, allergens such as pollen, environmental stress, etc.), and the mechanisms behind various cell aging phenomena, cell damage, and its prevention and recovery have been elucidated.

[0003] Active ingredients for preventing and improving the above-mentioned cell damage and aging phenomena have been proposed, and cosmetics, foods, beverages, and pharmaceuticals containing these active ingredients are on the market. Examples of such active ingredients include antioxidants such as vitamin C, vitamin E, catalase, and derivatives thereof; anti-inflammatory agents such as glycyrrhizinic acid or its salts, allantoin, and tranexamic acid; various ultraviolet absorbers; cell activating components such as α-hydroxycarboxylic acids, placental extracts, and γ-amino-β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin, and hyaluronic acid or its salts; moisturizing agents such as urea; and protein glycation inhibitors such as aminoguanidine.

[0004] However, these conventional ingredients have problems in terms of stability, safety, and efficacy when actually applied to a living body. For example, ascorbic acid or its derivatives are easily oxidized by external factors such as ultraviolet light or enzymes in the body, and their uptake into cells decreases with age. DISCLOSURE OF THE INVENTION [Problem to be solved by the invention]

[0005] As a result of intensive research to solve the above problems, the inventors have also found that fermented products of plants of the genus Nelumbo in the family Nelumbonaceae, hydrolysates of plants of the genus Oryza in the family Poaceae, extracts of plants of the genus Salicornia in the family Chenopodiaceae, and hydrolysates of soybeans or their extracts have the effect of promoting the synthesis of the vitamin C transporter (SVCT), a vitamin C channel involved in the uptake of vitamin C into cells.

[0006] Conventionally, inventions using fermented products of plants of the genus Nelumbo in the family Nelumbo, hydrolysates of plants of the genus Oryza in the family Poaceae, extracts of plants of the genus Salicornia in the family Chenopodiaceae, and hydrolysates of soybeans or extracts thereof as topical skin preparations have been known, for example, from Patent Documents 1 to 5. However, it was not known that fermented products of plants of the genus Nelumbo in the family Nelumbo, extracts or hydrolysates of plants of the genus Oryza in the family Poaceae, extracts of plants of the genus Salicornia in the family Chenopodiaceae, and hydrolysates of soybeans or extracts thereof have the effect of promoting the synthesis of SVCT1. [Patent Document 1] Patent Publication No. 2005-298489 [Patent Document 2] JP 2002-128632 A [Patent Document 3] Patent Publication No. 2013-103906 [Patent Document 4] Patent Publication No. 2005-145878 [Patent Document 5] JP 2006-290742 A [Means for solving the problem]

[0007] The present invention is a vitamin C uptake enhancer containing a hydrolysate of soybean or its extract as an active ingredient. The present invention is an external skin preparation containing the vitamin C uptake promoter. The present invention is an oral composition containing the vitamin C uptake enhancer. [Effects of the Invention]

[0008] The extract, hydrolysate or fermented product of the present invention can enhance the expression of SVCT-1, which is the vitamin C uptake site in pigment cells. Therefore, by using it in combination with vitamin C as an active ingredient for whitening or antioxidant purposes, it is possible to increase its uptake into pigment cells and obtain a more effective whitening effect. BEST MODE FOR CARRYING OUT THE INVENTION

[0009] The present invention will be described in detail below. Examples of plants of the genus Nelumbo in the family Nelumbonaceae that can be used in the present invention include Nelumbo nucifera and Nelumbo lutea.

[0010] When using a plant of the genus Lotus to obtain a fermented product using a microorganism (yeast, lactic acid bacteria, koji mold, or Bacillus subtilis), there are no particular limitations on the part of the plant that can be fermented, and any appropriate part such as the whole plant, leaves, flowers, stamens, pistils, stems, rhizomes, or seeds (grains) can be used, but from the standpoint of the effectiveness of the fermented product obtained, it is preferable to use the whole plant or seeds.

[0011] Furthermore, any plant of the genus Oryza in the family Poaceae (Poaceae) used in the present invention can be used as long as it is taxonomically classified as Oryza sativa. Specific examples include, but are not limited to, Koshihikari, Sasanishiki, Nihonbare, Akitakomachi, Kinuhikari, and Hanaechizen. The present invention does not limit the parts of the Oryza plant used, and any suitable part, such as the whole plant, leaves, flowers, stems, roots, or seeds (grains), can be used. However, the whole plant and leaves are preferred. When leaves are used, it is more preferable to use leaves before heading. When rice leaves are used, they may be heat-treated before extraction. This can suppress denaturation and deterioration due to active ingredients (enzymes, etc.) contained in the rice leaves, preventing the activity of harvested rice leaves from decreasing during storage.

[0012] Examples of plants of the genus Salicornia in the family Chenopodiaceae include, but are not limited to, Salicornia, Salicornia europaea, Salicornia bigelowii, Salicornia dolichostachia ssp. strictissima, etc. Parts of these plants that can be used include the whole plant, flowers, leaves, stems, seeds, etc.

[0013] The soybean used as a material in the present invention is a plant that is classified as the same species as soybean (scientific name: Glycine max (L.) Merill) in terms of botanical taxonomy. Any of white soybeans (yellow soybeans), green soybeans, and black soybeans can be used.

[0014] Extracts from the above plants can be prepared, for example, as follows. First, the part of each plant to be extracted is washed with water if necessary to remove foreign matter, and then, either as is or dried, shredded or crushed as necessary, and then contacted with an extraction solvent for extraction. Extraction can be carried out by contacting with the extraction solvent using a conventional method such as immersion, but supercritical extraction or steam distillation can also be used.

[0015] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; polyhydric alcohols such as ethylene glycol, 1,3-propanediol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, methyl propionate, and glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether, isopropyl ether, and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these can be used alone or in combination.

[0016] When a mixed solvent is used, the mixing ratio is preferably in the range of 1:1 to 25:1 in volume ratio (same hereinafter) for a mixed solvent of water and ethyl alcohol, 1:1 to 15:1 for a mixed solvent of water and glycerin, or 1:1 to 15:1 for a mixed solvent of water and 1,3-propanediol or 1,3-butylene glycol.

[0017] When preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, the extraction solvent may be blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid to adjust the pH to the desired level.

[0018] The extraction conditions, such as the extraction temperature and extraction time, vary depending on the type and pH of the solvent used. For example, when water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol, is used as the solvent, the extraction temperature is preferably in the range of 0°C to 90°C, and the extraction time is preferably 0.5 hours to 7 days.

[0019] Here, prior to or in parallel with the extraction treatment of the present invention, the extracted area may be subjected to hydrolysis treatment as needed, which may improve the skin irritation, efficacy, storage stability, etc. of the extract, thereby enabling more effective use of the extract.

[0020] In particular, in the present invention, extracts of rice leaves and soybeans are preferably subjected to hydrolysis treatment, which may be carried out by decomposition treatment using an acid, alkali, or enzyme.

[0021] When the hydrolysis treatment is carried out using an enzyme, at least one enzyme selected from the group consisting of protease, amylolytic enzyme, pectic enzyme, cellulose decomposing enzyme and lipolytic enzyme can be used.

[0022] Examples of proteolytic enzymes that can be used include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase, and aminopeptidase, bromelain, and complex proteases derived from microorganisms (e.g., Neurase [manufactured by Amano Enzyme Inc.]).

[0023] Examples of starch-degrading enzymes that can be used include α-amylase, β-amylase, glucoamylase, and β-galactosidase.

[0024] Examples of pectic enzymes that can be used include pectinase, pectin depolymerase, pectin demethoxylase, pectin lyase, pectin esterase, and polygalacturonase.

[0025] Examples of cellulose-degrading enzymes that can be used include cellulase, hemicellulase, agarase, mannase, chitinase, chitosanase, carrageenase, alginase, fucoidanase, inulase, xylanase, and ligninase.

[0026] As the lipid-degrading enzyme, for example, lipase, phospholipase, etc. can be used.

[0027] Furthermore, in the present invention, fermentation treatment with microorganisms (lactic acid bacteria, yeast, koji mold, Bacillus subtilis, etc.) may be carried out for the purpose of improving the effectiveness of the extracted material, suppressing skin irritation, improving stability, etc. In particular, in the present invention, it is preferable to carry out microbial fermentation treatment for plants of the genus Lotus or extracts thereof.

[0028] Fermentation can be carried out, for example, as follows. First, the plant itself (hereinafter sometimes referred to as the plant body) may be used as the assimilable source for fermentation, or an extract obtained by extracting the plant body with an appropriate medium may be used. Furthermore, when an extract is used, it is possible to carry out fermentation while still containing the plant body, without removing the plant body from the extract by solid-liquid separation. Here, the plant may be fresh, or may be dried or semi-dried in advance. Furthermore, the plant may be used as it is after harvesting.

[0029] In the present invention, lactic acid bacteria include, for example, lactic acid bacteria of the genus Lactobacillus such as Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, and Lactobacillus delbrueckii; lactic acid bacteria of the genus Carnobacterium such as Carnobacterium divergens and Carnobacterium piscicola; lactic acid bacteria of the genus Carnobacterium such as Leuconostoc mesenteroides, Leuconostoc lactis, and Leuconostoc citreum. Lactic acid bacteria of the genus Leuconostoc, such as Streptococcus citreum; lactic acid bacteria of the genus Streptococcus, such as Streptococcus faecalis and Streptococcus pyogenes; lactic acid bacteria of the genus Enterococcus, such as Enterococcus caseliflavus and Enterococcus sulfreus; lactic acid bacteria of the genus Lactococcus, such as Lactococcus plantarum and Lactococcus rafinolactis; lactic acid bacteria of the genus Veissella, such as Weissella confusa and Weissella kandleri; Atopobium Lactic acid bacteria of the genus Atopobium, such as Atopobium minutum and Atopobium parvulus;Examples of lactic acid bacteria include Vagococcus genus lactic acid bacteria such as Vagococcus fluvialis and Vagococcus salmoninarum; Pediococcus genus lactic acid bacteria such as Pediococcus damnosus and Pediococcus pentosaceus;

[0030] In the present invention, yeast includes, for example, yeasts of the genus Saccharomyces such as Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, and Saccharomyces bayonus; yeasts of the genus Galactomyces; yeasts of the genus Torulaspora such as Torulaspora delbruekii, Torulaspora fermentati, and Torulaspora rosei; yeasts of the genus Torulaspora such as Zygosaccharomyces rouxii and Zygosaccharomyces Yeasts of the genus Zygosaccharomyces, such as Zygosaccharomyces soya, Zygosaccharomyces sake, Zygosaccharomyces miso, and Zygosaccharomyces lactis; yeasts of the genus Candida, such as Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake, and Candida scottii; yeasts of the genus Aureobasidium pullulans, Aureobasidium mansonii, and Aureobasidium Examples of yeasts that can be used in the present invention include yeasts of the genus Aureobasidium, such as Aureobasideium microstictum. The yeasts of the present invention may be any of sake yeast, wine yeast, brewer's yeast, yeasts derived from plant flowers (rose, lily, cherry blossom, etc.), and yeasts derived from the sea.

[0031] In the present invention, examples of koji mold include yellow koji molds such as Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, and Aspergillus sojae; black koji molds such as Aspergillus awamori, Aspergillus kawauchii, Aspergillus usami, and Aspergillus niger; and red koji molds such as Monascus anka and Monascus pilosus.

[0032] In the present invention, examples of Bacillus subtilis include Bacillus natto, Bacillus subtilis, and Bacillus circulans.

[0033] When the above-mentioned suspension or extract is fermented with microorganisms, it is necessary to sterilize the material before the fermentation step to remove any unwanted bacteria that may hinder fermentation. This method of sterilizing and removing unwanted bacteria may involve washing the material for fermentation with sterilizing ethanol or the like beforehand and then suspending it in a sterile solvent such as sterile water. Alternatively, the material for fermentation may be suspended in a solvent and then sterilized by heat sterilization or the like. Commonly used heat sterilization methods include autoclave sterilization, in which the suspension is heated to 120-130°C for 10-20 minutes, and intermittent sterilization, in which the suspension is kept at 80-90°C for 60-120 minutes, repeated once a day for 2-3 days.

[0034] The sterilized suspension is placed in a fermentation tank, and the microorganisms are inoculated and fermented. The inoculation amount of the microorganisms is 10 7 ~10 8If the inoculum amount is greater than the above range, the fermentation time will not change significantly, while if it is less than the above range, it will take a long time to complete the fermentation, which is not desirable.

[0035] The fermentation temperature is generally in the range of 5 to 50°C, preferably in the range of 20 to 40°C, which is the optimum temperature for growth of each microorganism (for example, 30 to 40°C for lactic acid bacteria, and 25 to 30°C for yeast). The number of days for fermentation is generally in the range of 1 to 10 days, preferably 2 to 5 days, at the optimum temperature. If the number of days for fermentation is shorter than the above general range, the fermentation does not proceed sufficiently and the effectiveness of the fermented product tends to decrease. On the other hand, if the number of days is longer than 10 days, not only will no further increase in effectiveness be observed, but coloration and an increased fermentation odor will occur, both of which are undesirable.

[0036] When preparing a fermented product from a plant of the genus Lotus, the suspension or extract solution may be subjected to the above-mentioned hydrolysis treatment before or simultaneously with inoculation of the microorganism so that the components of the target use part can be more effectively utilized as a source of assimilation for the microorganism.

[0037] The extract, hydrolysate, or fermentation product prepared as described above may be used as is after generally adjusting the pH to 3 to 9, or may be used at a desired concentration by concentrating under reduced pressure, etc. Alternatively, it may be dried by a conventional method such as spray drying.

[0038] Furthermore, the extract, hydrolysate or fermented product prepared as described above may be stored in a refrigerator for a certain period of time in order to improve storage stability, and the supernatant may then be used.

[0039] The extract, hydrolysate, or fermented product according to the present invention can be incorporated into external skin preparations (cosmetics, quasi-drugs, external pharmaceuticals), or food and beverage products (oral compositions) for beauty or health promotion. Examples of external skin preparations include emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, blushers, face powders, facial cleansers, body shampoos, scalp and hair shampoos, hair conditioners, shampoos or tonics for hair growth and care, cleansing cosmetics such as soaps, and bath additives, but the present invention is not limited to these. In addition, as food and beverage products for beauty or health promotion, the compound can be blended into beverages such as beauty drinks, nutritional drinks, sports drinks, near water, vitamin drinks, mineral drinks, and alcoholic drinks; foods such as various soups (including powdered soups), dairy products, jellies, candies, candy tablets, and gum; and health foods and beverages in tablet, liquid, granular, or jelly form, but the present invention is not limited to these, and the compound can be blended into foods and beverages that can be taken orally.

[0040] The amount of the extract, hydrolysate, or fermented product according to the present invention can be adjusted appropriately depending on the formulation to be used. For example, the solid content ranges from 0.002 to 1.0 wt% (solid content weight %, the same applies hereinafter) for skin care cosmetics, 0.002 to 1.0 wt% for makeup cosmetics, and 0.002 to 10.0 wt% for cleansing cosmetics. Furthermore, for hair cosmetics, the solid content ranges from 0.0001 to 5.0 wt%. Furthermore, the amount of the extract, hydrolysate, or fermented product in oral compositions is preferably from 0.1 to 15 wt% in terms of solid content.

[0041] In addition to the extract, hydrolysate, or fermentation product of the present invention, preparations containing the extract, hydrolysate, or fermentation product may contain, as needed, ingredients typically used in topical skin preparations or oral compositions, such as oily ingredients, surfactants (synthetic or natural), moisturizers, thickeners, preservatives / bactericides, powder ingredients, antioxidants, chelating agents, pH adjusters, colorants, fragrances, etc. Furthermore, as long as the efficacy and characteristics are not impaired, there is no problem with using them in combination with other physiologically active ingredients.

[0042] Examples of oily components include plant-derived oils such as lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, coconut oil, chamomile oil, palm oil, cacao oil, meadowfoam oil, rosehip oil, lambender oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, and plant-derived squalane; animal-derived oils such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin oil. Examples of suitable glycerin include hydrocarbons such as ginseng wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, and stearyl alcohol; and synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0043] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of surfactants that can be used include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, and N-acylamidopropyl-N',N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0044] Examples of emulsifiers or emulsifying aids that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or derivatives thereof, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble polysaccharides, soybean-derived protein and polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), and the like.

[0045] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, Bletilla striata root (white extract), and various amino acids and their derivatives.

[0046] Examples of thickeners include ingredients derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; extracts of Bletilla striata (white algae); polysaccharides such as pectin, locust bean gum, aloe polysaccharides, and Alcaligenes-produced polysaccharides; gums such as xanthan gum, tragacanth gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymers, and acrylic acid / methacrylic acid copolymers; hyaluronic acid and its derivatives; and polyglutamic acid and its derivatives.

[0047] Examples of antiseptics and disinfectants include urea; parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazolidinyl urea), polyphosphoric acid, propanediol, 1,2-pentanediol, various essential oils, bark dry distillate, radish fermented liquid, and ethanol or 1,3-butylene glycol derived from plants such as sugarcane.

[0048] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybeans, adzuki beans, etc.), etc.

[0049] Examples of ultraviolet absorbers include ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0050] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, extract of Barberry, extract of Bletilla striata root, extract of peony, vitamin E and its derivatives (e.g., vitamin E nicotinate, vitamin E linoleate, etc.).

[0051] Examples of chelating agents include trisodium ethylenediaminehydroxyethyltriacetate, edetic acid or its salts, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, and tetrasodium hydroxyethanediphosphonate.

[0052] Examples of pH adjusters include citric acid or salts thereof, lactic acid or salts thereof, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide, and potassium hydroxide.

[0053] Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, and AMP (adenosine monophosphate), which may be blended alone or in combination.

[0054] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid esters such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate. Ascorbic acid sugar derivatives such as L-ascorbic acid esters, L-ascorbic acid 2-glucoside, L-ascorbic acid 5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, and the like; 6-acylated products of these ascorbic acid sugar derivatives (the acyl group is a hexanoyl group, an octanoyl group, a decanoyl group, etc.); L-ascorbic acid such as L-ascorbic acid tetraisopalmitate and L-ascorbic acid tetralaurate. Examples of hydroquinone derivatives include tetra-fatty acid esters, 3-O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-sodium palmitate, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, and isostearyl ascorbyl phosphate. Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside). amide), α-arbutin (hydroquinone-α-D-glucopyranoside), etc.; tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), tranexamic acid amides (e.g., tranexamic acid methylamide), etc.; resorcinol derivatives include, for example, 4-n-butylresorcinol, 4-isoamylresorcinol, etc.; 2,5-dihydroxybenzoic acid derivatives include, for example, 2,Examples of nicotinic acid derivatives include nicotinamide and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0055] Examples of physiologically active ingredients include placenta extract, mulberry bark extract, saxifrage extract, perilla extract, rice bran extract or hydrolysate thereof, white mustard extract or hydrolysate thereof, fermented white mustard, peony extract or hydrolysate thereof, lactic acid bacteria-fermented rice, barley extract, lotus flower extract, Job's tears hydrolysate, Job's tears seed fermented product, royal jelly fermented product, sake lees extract or ceramide contained therein, sake lees fermented product, Pandanus amaryllifolius (Pandanus amaryllifolius Roxb.) extract, Arcangelicia flava Merrilli extract, and chamomile extract. Also, eggplant (lotus, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or its hydrolysate, apricot fruit extract, extract of seaweed such as Katamen Euonymus rhododendron, extract of marine flowering plants such as Zostera marina, fermented soy milk, jellyfish water, rice extract or its hydrolysate, fermented rice extract, germinated rice extract or its hydrolysate, fermented germinated rice, black soybean extract or its hydrolysate, extract of damask rose flower, extract of bamboo shoot skin, linoleic acid and its derivatives or processed products (for example, liposomal linoleic acid, etc.), collagen derived from animals or fish and its derivatives Derivatives, elastin and its derivatives, glycyrrhizinic acid and its derivatives (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, gamma-amino-beta-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or its fermented product, red ginseng extract, Mitsuishi Kami extract, loofah extract, Ulva pertusa extract, peach extract, peach kernel extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract, pau d'arco bark extract, hibiscus flower extract or fermented product, wormwood extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract, Benifuki extract, Bletilla serrata extract, extract or hydrolysate of Japanese pepper peel or seed coat, safflower flower extract, Casablanca extract, sweet potato extract or its fermented product, guava leaf extract, Houttuynia cordata extract, Banpeiyu extract, aloe extract, fig flower extract, apple extract, white asparagus extract, etc.

[0056] The present invention will now be described in more detail with reference to Production Examples, Test Examples, and Formulation Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all % mean % by weight.

[0057] Production Example 1. Fermented lotus seed solution (1) 100 g of lotus seeds (with the astringent skin removed) were crushed and 1900 g of purified water was added to prepare a suspension, which was then heat-sterilized. 100 g of lactic acid bacteria (Lactobacillus plantarum) was added to the suspension. 8 The cells were inoculated at a concentration of 1 / mL and cultured statically for 3 days at 37°C under a nitrogen atmosphere. After the culture was completed, the culture was sterilized by heating and filtered to obtain 1415 g of a solution of lotus seeds fermented by lactic acid bacteria (solid concentration 2.53%).

[0058] Production Example 2. Fermented lotus seed solution (2) 1,430 g of a solution of lotus seeds fermented with yeast (solid concentration 2.24%) was obtained in the same manner as in Production Example 1, except that the yeast Saccharomyces cerevisiae was used as the microorganism instead of the lactic acid bacterium (Lactobacillus plantarum).

[0059] Preparation Example 3. Fermented lotus seed solution (3) The same procedure as in Production Example 1 was carried out except that the koji mold Aspergillus oryzae was used as the microorganism instead of the lactic acid bacterium Lactobacillus plantarum, to obtain 1,420 g of a solution of lotus seeds fermented with koji mold (solid concentration 2.41%).

[0060] Preparation Example 4. Fermented lotus seed solution (4) The same procedure as in Production Example 1 was carried out except that Bacillus natto, a Bacillus subtilis bacterium, was used as the microorganism instead of lactic acid bacteria (Lactobacillus plantarum), to obtain 1,385 g of a solution of lotus seeds fermented with Bacillus subtilis (solid concentration 2.60%).

[0061] Production Example 5. Fermented lotus whole plant solution (1) Except for using 100 g of finely chopped whole lotus plants instead of crushed lotus seeds as the fermentation material, the same procedure as in Production Example 1 was carried out to obtain 1,167 g of a lactic acid bacteria fermentation product solution of whole lotus plants (solid concentration 1.21%).

[0062] Preparation Example 6: Rice leaf hydrolysate solution (1) 1000 g of purified water was added to 200 g of dried, ground rice leaves immediately before heading (booting stage), and extraction was carried out at 80°C for 1 hour, followed by filtration, yielding 550 g of a pale yellow, transparent rice leaf extract solution (solid concentration 2.5%). 0.025 g of pectinase was added to 500 g of the resulting extract solution, and hydrolysis was carried out at 40°C for 4 hours. The solution was then heated at 90°C for 1 hour to inactivate the enzyme, and then filtered, yielding 455 g of a pale yellow, transparent enzymatic hydrolysate solution of rice leaf extract (solid concentration 2.72%).

[0063] Preparation Example 7. Rice leaf hydrolysate solution (2) The same procedure as in Production Example 1 was carried out except that cellulase was used instead of pectinase, to obtain 450 g of an enzymatic hydrolysate solution of rice leaf extract (solid concentration: 2.31%).

[0064] Preparation Example 8: Rice leaf hydrolysate solution (3) The same procedure as in Production Example 1 was carried out except that xylanase was used instead of pectinase, to obtain 452 g of an enzymatic hydrolysate solution of rice leaf extract (solid concentration: 2.43%).

[0065] Preparation Example 9. Preparation of Salicornia herb extract (1) 200 g of purified water was added to 20 g of dried, shredded Salicornia herbacea whole plant, and the mixture was extracted for 1 hour at 40° C. The resulting extract was filtered to obtain 155 g of a brown, transparent extract solution (solid content: 2.00%).

[0066] Preparation Example 10. Preparation of Salicornia herb extract (2) 200 g of purified water was added to 20 g of dried, shredded Salicornia stems, and the mixture was extracted for 3 hours at 40° C. The resulting extract was filtered to obtain 152 g of a brown, transparent extract solution (solid content: 1.99%).

[0067] Preparation Example 11. Preparation of Salicornia herb extract (3) 20 g of dried, shredded Salicornia herb was added to 20 g of a 50% aqueous solution of 1,3-butylene glycol, and extracted for 5 hours at 40° C. The resulting extract was filtered to obtain 160 g of a light brown, transparent extract solution (solid content: 1.95%).

[0068] Production example 12. Soybean hydrolyzate 200 g of purified water was added to 10 g of dried and ground black soybean seeds (black soybeans), and the mixture was extracted at 80°C for 1 hour. The resulting extract was roughly filtered, and Neurase (Amano Enzyme Inc.) was added to the mixture to a concentration of 0.01% and allowed to react at 40°C for 3 hours. The enzyme was then inactivated by treatment at 80°C for 1 hour, and the mixture was then filtered to obtain 160 g of a light brown, transparent black soybean extract hydrolysate solution (solids concentration 1.2%).

[0069] Prescription example 1. Emulsion [Ingredients] Part Liquid Paraffin 6.0 Hexalan 4.0 Jojoba oil 1.0 Lotus essential oil 0.025 Polyoxyethylene (20) Sorbitan Monostearate 1.0 Lipophilic glyceryl stearate 1.0 Hydrogenated soy lecithin 1.5 Fermented product of Production Example 1 3.0 L-Ascorbic Acid 2-Glucoside 2.0 Potassium hydroxide 0.5 Glycerin 3.0 1,3-butylene glycol 2.0 Carboxymethylcellulose 0.3 Sodium hyaluronate 0.01 Water-soluble collagen 0.1 Fragrance (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0070] Prescription example 2: Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 3.0 parts of the fermented product of Production Example 2 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

[0071] Prescription example 3. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 3.0 parts of the fermented product of Production Example 3 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

[0072] Prescription example 4: Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 3.0 parts of the fermented product of Production Example 4 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

[0073] Prescription example 5. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 3.0 parts of the fermented product of Production Example 5 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

[0074] Prescription example 6. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolysate of Production Example 6 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

[0075] Prescription example 7. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolysate of Production Example 7 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

[0076] Prescription example 8. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolysate of Production Example 8 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

[0077] Prescription example 9. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract from Production Example 9 was used in place of 3.0 parts of the fermented product from Production Example 1 contained in Formulation Example 1.

[0078] Prescription example 10. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 10 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

[0079] Prescription example 11. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 11 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

[0080] Prescription example 12. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolysate of Production Example 12 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

[0081] Prescription example 13. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 2.0 parts of L-ascorbic acid 2-phosphate magnesium ester were used in place of 2.0 parts of L-ascorbic acid 2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 1.

[0082] Prescription example 14. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 2.0 parts of sodium L-ascorbic acid 2-phosphate was used in place of 2.0 parts of L-ascorbic acid 2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 1.

[0083] Prescription example 15. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 1 were replaced with 1.0 part of 3-O-ethyl ascorbic acid.

[0084] Prescription example 16. Emulsion [Ingredients] Part Squalane 3.0 Behenyl Alcohol 3.0 Hexalan 4.0 Jojoba oil 1.0 Polyoxyethylene (20) Sorbitan Monostearate 1.0 Glycerin fatty acid ester 1.0 Soy lecithin 1.5 Fermented product of Production Example 1 5.0 L-Ascorbic Acid 2-Glucoside 2.0 Potassium hydroxide 0.5 Dipotassium glycyrrhizinate 0.1 Glycerin 3.0 1,3-butylene glycol 2.0 Water-soluble collagen 0.1 Sodium hyaluronate 0.01 Purified water (enough to make the total volume 100 parts)

[0085] Prescription example 17. Emulsion An emulsion was obtained in the same manner as in Formulation Example 16, except that 1.0 part of tranexamic acid was used in place of 1.0 part of dipotassium glycyrrhizinate contained in Formulation Example 16.

[0086] Prescription example 18. Lotion [Ingredients] Part Jojoba oil 1.0 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Butylparaben 0.1 Fermented product of Production Example 1 5.0 L-Ascorbic Acid 2-Glucoside 2.0 Potassium hydroxide 0.5 Glycerin 5.0 1,3-butylene glycol 5.0 Potassium hydroxide (appropriate amount) Fragrance (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0087] Prescription example 19. Lotion [Ingredients] Part Fermented product of Production Example 2 10.0 Ethanol 10.0 Glycerin 3.0 1,3-butylene glycol 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Carboxyvinyl polymer 0.1 Xanthan gum 0.1 Guar gum 0.1 Fragrance (appropriate amount) Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0088] Prescription example 20. Lotion A lotion was obtained in the same manner as in Formulation Example 19, except that 10.0 parts of the hydrolysate of Production Example 6 was used in place of the fermentation product of Production Example 2 contained in the ingredients of Formulation Example 19.

[0089] Prescription example 21. Essence [Ingredients] Part Ethanol 2.0 Glycerin 5.0 1,3-butylene glycol 5.0 Methylparaben 0.1 Hyaluronic acid 0.1 Hydrolyzed Hyaluronic Acid Liquid 0.1 Extract of Preparation Example 9 5.0 Citric acid 0.3 Sodium citrate 0.6 Purified water (enough to make the total volume 100 parts)

[0090] Example 22. Liquid foundation [Ingredients] Part Stearic acid 2.4 Propylene glycol monostearate 2.0 Cetostearyl alcohol 0.2 Liquid Lanolin 2.0 Liquid Paraffin 3.0 Isopropyl myristate 8.5 Propylparaben 0.05 Fermented product of Production Example 4 5.0 Sodium carboxymethylcellulose 0.2 Bentonite 0.5 Propylene Glycol 4.0 Triethanolamine 1.1 Methylparaben 0.1 Purified water (enough to make the total volume 100 parts) Titanium dioxide 8.0 Talc 4.0 Color pigment (appropriate amount)

[0091] Prescription example 23. Body shampoo [Ingredients] Part Sodium N-lauroylmethylalanine 25.0 Potassium coconut oil fatty acid solution (40%) 26.0 Coconut oil fatty acid diethanolamide 3.0 Methylparaben 0.1 Hydrolyzate of Production Example 7 5.0 1,3-butylene glycol 2.0 Purified water (enough to make the total volume 100 parts)

[0092] Prescription example 24. Hair shampoo [Ingredients] Part Sodium N-cocoyl methyl taurate 10.0 Sodium polyoxyethylene (3) alkyl ether sulfate 20.0 Lauryl dimethylaminoacetic acid betaine 10.0 Coconut oil fatty acid diethanolamide 4.0 Methylparaben 0.1 Citric acid 0.1 Extract of Preparation Example 10 2.0 1,3-butylene glycol 2.0 Purified water (enough to make the total volume 100 parts)

[0093] Prescription example 25. Hair conditioner [Ingredients] Part Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Cetyl alcohol 3.2 Stearyl Alcohol 1.0 Methylparaben 0.1 Hydrolyzate of Production Example 8 2.0 1,3-butylene glycol 5.0 Purified water (enough to make the total volume 100 parts)

[0094] Prescription Example 26. Beverages [Ingredients] Part Hydrolyzate of Production Example 6 5.0 Citric acid 0.1 Sweetener (sucrose) 0.01 Vitamin C 0.1 Purified water (enough to make the total volume 100 parts)

[0095] Prescription Example 27. Beverages A beverage was obtained in the same manner as in Formulation Example 26, except that the hydrolysate of Production Example 9 was used instead of the fermented product of Production Example 1 contained in Formulation Example 26.

[0096] Prescription Example 28. Beverages A beverage was obtained in the same manner as in Formulation Example 26, except that the hydrolysate of Production Example 12 was used instead of the fermented product of Production Example 1 contained in Formulation Example 26.

[0097] Test Example 1. Sodium-dependent vitamin C transporter (SVCT) synthesis promotion effect Normal human epidermal melanocytes were cultured at 1 × 10 in DermaLife® (Kurabo) containing a proliferation additive. 5The cells were prepared at a concentration of 100 μL / mL, and 100 μL of each was seeded into a 96-well microplate. The cells were cultured at 37°C under 5% carbon dioxide and saturated steam. After 24 hours, culture solutions containing the fermented products of Production Examples 1 and 2, the hydrolysates of Production Examples 6 and 7, and the extracts of Production Examples 9 and 10, respectively, were added as sample solutions 1, and the cells were further cultured. Sample solutions 1 were prepared so that their final concentrations in the culture solution were 0.25%, 0.5%, and 1.0%. Sample solution 2 was prepared so that the final concentration in the culture solution was 2.0%. Controls included Control 1, where a culture solution containing PBS(-) (1.0%) was added instead of Sample Solution 1, and Control 2, where a culture solution containing PBS(-) (2.0%) was added instead of Sample Solution 2. After 48 hours, the culture supernatant was removed, and 200 μL of PBS(-) was added and removed. 50 μL of 10% trichloroacetic acid (Wako Pure Chemical Industries, Ltd.) was added and incubated in the cold for 30 minutes, after which the supernatant was removed. The cells were washed with 100 μL of PBS(-), and 50 μL of 0.2% Triton-X-containing PBS(-) was added and incubated at room temperature for 1 hour. The supernatant was removed, and 50 μL of 8% bovine serum albumin (Sigma)-containing PBS(-) was added and incubated at room temperature for 2 hours. The supernatant was removed, and the cells were washed with 100 μL of 0.2% Triton-X-containing PBS(-). 50 μL of anti-SVCT mouse monoclonal antibody (Santa Cruz) was added and incubated in the cold for 24 hours. The supernatant was removed, and the cells were washed three times with 100 μL of 0.2% Triton-X-containing PBS(-). 50 μL of Alexa Fluor 488 anti-mouse secondary antibody (Life Technologies) was added and incubated at room temperature in the dark for 2 hours. The supernatant was removed and the cells were washed three times with 100 μL of 0.2% Triton-X-containing PBS(-). 100 μL of PBS(-) was added to each well and the fluorescence intensity of Ex485 / Em520 was measured using a fluorescence plate reader (Dainippon Pharmaceutical). The relative fluorescence intensity to the control value was used as the SVCT synthesis rate (%).

[0098] The results of Test Example 1 for Sample Solution 1 are shown in Table 1. [Table 1] JPEG0007770019000001.jpg192130

[0099] As shown in Table 1, it was confirmed that the extract, hydrolysate or fermented product according to the present invention has a significantly superior effect of promoting SVCT synthesis in a concentration-dependent manner.

[0100] The results of Test Example 1 for Sample Solution 2 are shown in Table 2. [Table 2] JPEG0007770019000002.jpg38130

[0101] As shown in Table 2, it was confirmed that the hydrolysate according to the present invention has a significantly superior effect of promoting the synthesis of SVCT.

Claims

[Claim 1] A vitamin C uptake enhancer for topical use on the skin, containing as an active ingredient an enzymatic hydrolysate of the protein content of black soybean seed extract.

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