Antibacterial composition

Abstract

According to an embodiment of the present invention, there is provided an antibacterial composition comprising Lemongrass Oil, Lavender Oil, and Ylang Ylang Oil.

Description

[Technical Field]

[0001] The present invention relates to an antibacterial composition. [Background technology]

[0002] Recently, there has been a great deal of interest in the development of antibacterial substances derived from natural products. These antibacterial substances are known to exhibit high antibacterial effects due to the synergistic action of various components derived from natural products, and often have low toxicity to humans or animals.

[0003] Antibacterial substances also play an important role in the fields of medicine and cosmetics, and are used in many cosmetics to solve problems related to skin diseases. For example, many antibacterial cosmetics have been developed to improve skin problems such as hair loss, dandruff, and acne, and these cosmetics can minimize the growth of harmful bacterial strains present on the skin using antibacterial substances. Summary of the Invention [Problem to be solved by the invention]

[0004] The present invention has been made to solve the above problems, and an object of the present invention is to provide an antibacterial composition.

[0005] The technical problems of the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art from the following description. [Means for solving the problem]

[0006] According to an embodiment of the present invention, there is provided an antibacterial composition comprising lemongrass oil, lavender oil, and ylang ylang oil.

[0007] The weight ratio of the lavender oil to the lemongrass oil may be 1:1.3 to 2.7.

[0008] The weight ratio of the ylang-ylang oil to the lemongrass oil may be 1:4-8.

[0009] The weight ratio of the ylang-ylang oil to the lavender oil may be 1:1.5 to 4.5.

[0010] The weight ratio of the ylang-ylang oil, the lavender oil, and the lemongrass oil may be 1:1.5-4.5:4-8.

[0011] In addition, the composition may have antibacterial activity against at least one of strains of the genus Staphylococcus, strains of the genus Streptococcus, strains of the genus Malassezia, strains of the genus Propionibacterium, and strains of the genus Candida.

[0012] According to an embodiment of the present invention, a cosmetic composition including an antibacterial composition is provided.

[0013] According to an embodiment of the present invention, an external skin preparation composition containing an antibacterial composition is provided.

[0014] According to an embodiment of the present invention, there is provided a pharmaceutical composition for preventing or treating hair loss, comprising an antibacterial composition. [Effects of the Invention]

[0015] The antibacterial composition according to the present invention contains lemongrass oil, lavender oil and ylang-ylang oil, and can provide excellent antibacterial or antifungal effects against harmful strains of bacteria that cause symptoms such as hair loss, acne or dandruff.

[0016] Furthermore, the antibacterial composition according to the present invention can alleviate symptoms such as hair loss, acne, or dandruff, and promote hair growth.

[0017] A brief description of each of the drawings referred to in the detailed description of the present invention is provided to provide a more complete understanding of the drawings. [Brief explanation of the drawings]

[0018] [Figure 1] 1 shows photographs showing the results of a Minimal Inhibitory Concentration (MIC) measurement experiment according to the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by a skilled artisan in the art to which the present invention belongs. Generally, the nomenclature used herein is well known and commonly used in the art. Furthermore, when describing embodiments of the present invention, if a detailed description of related known structures or functions is deemed to hinder understanding of the embodiments of the present invention, such detailed description will be omitted. Furthermore, although embodiments of the present invention will be described below, the technical concept of the present invention is not limited thereto and may be modified and implemented in various ways by those skilled in the art.

[0020] As used herein, when any part is said to include a certain element, this does not mean excluding other elements, but may further include other elements, unless specifically stated to the contrary. As used herein, the term "and / or" includes a combination of multiple associated items or any one item of multiple associated items.

[0021] In the present invention, "oil" refers to a natural vegetable oil extracted from a plant, and is used interchangeably with "essence," "distilled extract," or "essential oil." The plant used to produce the oil may be an intact plant that has not been crushed or ground, or may be cut into pieces that have been crushed or ground, and may include all parts of the plant, such as roots, leaves, stems, flowers, and fruits.

[0022] In the present invention, the oil can be extracted using steam distillation, which is a method of extracting aromatic active ingredients from plants using steam and then condensing them by cooling with cooling water. The oil also includes the extract itself and all formulations that can be made using the extract, such as diluted or concentrated extracts obtained by steam distillation of plants, dried products obtained by drying the extract, crude or purified products of the extract, and mixtures thereof, but is not limited to these.

[0023] In the present invention, the oil may be in a liquid form, a solid form obtained by drying a liquid extract, or a powder form obtained by pulverizing a solid extract. In this case, various drying methods commonly used in the art, such as spray drying, freeze drying, vacuum drying, and hot air drying, may be applied, but are not limited to these.

[0024] According to an embodiment of the present invention, an antibacterial composition containing lemongrass oil, lavender oil, and ylang-ylang oil is provided. By using a combination of these three oils, the antibacterial composition according to the embodiment of the present invention can have a stronger antibacterial effect than when only one or two oils are used, due to the synergistic effect of each oil.

[0025] Lemongrass oil is an oil extracted from Cymbopogon Schoenanthus, also known as Cymbopogon Schoenanthus Oil, and is known to have antifungal, disinfectant, deodorizing, and bactericidal properties.

[0026] In an embodiment, the antibacterial composition may contain 30-85 wt. % lemongrass oil, specifically 43-75 wt. % lemongrass oil, more specifically 45-70 wt. % lemongrass oil, and even more specifically 50-70 wt. % lemongrass oil, based on the total weight of the antibacterial composition.

[0027] Lavender oil is an oil extracted from lavender (Lavandula angustifolia) and is known to have antibacterial, anti-inflammatory, and antioxidant properties, as well as to protect the skin from external harmful substances and to have excellent soothing and circulatory effects.

[0028] In an embodiment, the antibacterial composition may contain 10 to 50% by weight, more specifically 15 to 45% by weight, and even more specifically 20 to 40% by weight of lavender oil, based on the total weight of the antibacterial composition.

[0029] Ylang-ylang oil is an oil extracted from the ylang-ylang tree (Cananga odorata) and is known to help balance sebum secretion, relieve skin and scalp symptoms caused by excessive sebum secretion, and strengthen hair.

[0030] In an embodiment, the antibacterial composition may contain ylang-ylang oil in an amount of 1 to 30% by weight, specifically 3 to 25% by weight, or more specifically 5 to 20% by weight, based on the total weight of the antibacterial composition.

[0031] For example, by including lemongrass oil, lavender oil, and / or ylang-ylang oil in the above content ranges, excellent antibacterial properties, fragrance, usability, etc. can be realized, but the present invention is not limited to these.

[0032] In an embodiment, the weight ratio of lavender oil to lemongrass oil may be 1:1.3-2.7. Additionally / optionally, the weight ratio of ylang-ylang oil to lemongrass oil may be 1:4-8. Additionally / optionally, the weight ratio of ylang-ylang oil to lavender oil may be 1:1.5-4.5. When the weight ratio of lemongrass oil, lavender oil, and / or ylang-ylang oil is within the above range, the antibacterial activity can be improved. However, the present invention is not limited to these.

[0033] In an embodiment, the weight ratio of ylang-ylang oil, lavender oil, and lemongrass oil may be 1:1.5-4.5:4-8, specifically 1:2-4:4-8, and more specifically 1:2.5-3.5:4-8. When the weight ratio of lemongrass oil, lavender oil, and ylang-ylang oil is within the above range, antibacterial activity is maximized, hair loss is minimized, and excellent hair growth can be achieved. However, the range is not limited to these.

[0034] In embodiments, the antibacterial composition may have antibacterial activity against harmful strains of bacteria that cause symptoms such as hair loss, acne, dandruff, etc. For example, the antibacterial composition may have antibacterial activity against at least one of strains of the genus Staphylococcus, strains of the genus Streptococcus, strains of the genus Malassezia, strains of the genus Propionibacterium, and strains of the genus Candida, and specifically may have antibacterial activity against strains of the genus Staphylococcus.

[0035] In embodiments, the antibacterial composition may have antibacterial activity against at least one of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Malassezia furfur, Propionibacterium acnes, and Candida albicans, and specifically may have antibacterial activity against Staphylococcus aureus.

[0036] According to an embodiment of the present invention, a cosmetic composition is provided, which may include the antibacterial composition described above.

[0037] In the embodiment, the antibacterial composition has antibacterial activity against harmful strains of bacteria that cause symptoms such as hair loss, acne, or dandruff, and therefore the cosmetic composition according to the embodiment of the present invention can alleviate symptoms such as hair loss, acne, or dandruff and promote hair growth.

[0038] In the examples, the cosmetic composition may further contain additives commonly used in the art within the scope of the present invention. For example, the cosmetic composition may further contain thickeners, pH adjusters, isotonicity agents, surfactants, stabilizers, preservatives, antiseptics, bactericides, moisturizers, blocking agents, antioxidants, organic pigments, inorganic pigments, fragrances, vitamins, etc. The amounts of the above ingredients can be easily selected by those skilled in the art within the scope of the present invention, provided that the objectives and effects of the present invention are not impaired.

[0039] In embodiments, the cosmetic composition may be provided in a dosage form commonly produced in the art. For example, the cosmetic composition may be formulated as an oil dispersion, a water dispersion, an oil-in-water (O / W), a water-in-oil (W / O), a water-in-oil-in-water (W / O / W), or an oil-in-water-in-oil (O / W / O) emulsion, a solubilized dosage form, etc. However, the cosmetic composition may also be provided in a dosage form such as a solution, a suspension, an emulsion, a solid, a gel, an oil, a powder, a paste, or a foam aerosol.

[0040] In embodiments, the cosmetic composition may be formulated into a variety of products. For example, it may be formulated into hair cleansing products such as shampoo, rinse, hair conditioner, and hair gel, or scalp and / or hair care products such as scalp or hair toner, scalp or hair essence, scalp or hair serum, and scalp or hair tonic. It may also be formulated into soap, cleanser, cleansing cream, cleansing water, or color cosmetics such as lipstick, lip balm, mascara, blusher, shading, highlighter, makeup base, foundation, makeup powder, concealer, and skin cover. It may also be formulated into functional cosmetics such as nourishing creams, essences, and ampoules, or basic cosmetics such as toners and lotions. It may also be formulated into products that adhere to the skin, such as packs and hydrogel slimming patches, or products that are sprayed onto the skin, such as mists and aerosols.

[0041] An embodiment of the present invention provides a skin topical composition, which may include the antibacterial composition described above.

[0042] The topical skin composition refers to a topical preparation that acts on the skin, and may include, but is not limited to, pharmaceutical products, quasi-drugs, etc. For example, the topical skin composition may be in the form of a cream, gel, skin emulsion, patch, spray, etc.

[0043] In the examples, the topical skin preparation composition may contain, as needed, ingredients commonly used in topical skin preparations such as cosmetics and pharmaceuticals, such as aqueous ingredients, oily ingredients, powdery ingredients, alcohols, moisturizers, thickeners, UV protection agents, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, fatty substances, solubilizers, thickeners, gelling agents, softeners, foaming agents, fragrances, sequestering agents, chelating agents, vitamins, various skin nutrients, or combinations thereof. The amounts of the ingredients can be easily selected by those skilled in the art within ranges that do not impair the objectives and effects of the present invention.

[0044] According to an embodiment of the present invention, there is provided a pharmaceutical composition for preventing or treating hair loss. The pharmaceutical composition may comprise an antibacterial composition as described above. "Preventing hair loss" means preventing, suppressing, inhibiting, and reducing partial or complete hair loss.

[0045] " Hair loss " refers to the loss of hair growth and partial or total hair loss, and may include, for example, androgenic alopecia or androgenetic alopecia, toxic alopecia, androgenetic alopecia, alopecia areata, telogen effluvium, alopecia caused by endocrine disorder, metabolic disorder, nutritional disorder, drug-induced alopecia, mechanical alopecia, alopecia associated with skin disease, cicatricial alopecia, congenital alopecia, trichotillomania. For example, alopecia can have many etiologies, including hereditary factors, aging, local and systemic disease, fever symptoms, mental stress, hormonal problems and drug side effects, etc.

[0046] In some embodiments, the pharmaceutical composition can promote hair growth. "Hair growth" can include maintaining, inducing, stimulating, promoting, and restoring hair development; growing defective hair, extending the anagen phase of the hair growth cycle, or converting vellus hair to adult hair. "Promoting hair growth" also refers to promoting hair growth, extending the anagen phase of the hair growth cycle, or converting vellus hair, which is thin, smooth, short, and bleached hair whose roots are located on the surface of the dermis, to adult hair, which is thick, long, and pigmented hair whose roots are deep within the dermis.

[0047] In an embodiment, the pharmaceutical composition may further contain a pharmaceutically acceptable salt within a range that does not impair the effects of the present invention. Here, the "pharmaceutically acceptable salt" may be a salt that does not induce serious irritation to an organism to which the compound is administered and does not impair the biological activity and physical properties of the compound.

[0048] For example, pharmaceutically acceptable salts can be formed from inorganic acids, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, or organic acids, including acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, N-acetylcysteine, and the like.

[0049] For example, pharmaceutically acceptable salts can be prepared by adding an inorganic or organic base to the free acid. Salts derived from inorganic bases may include, but are not limited to, sodium, potassium, lithium, ammonium, calcium, magnesium salts, etc. Salts derived from organic bases include, but are not limited to, salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines, and basic ion exchange resins, such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, lysine, arginine, N-ethylpiperidine, piperidine, polyimine resins, and the like.

[0050] In an embodiment, the pharmaceutical composition may further contain a pharmaceutically acceptable carrier or additive within a range that does not impair the effects of the present invention. Here, the "pharmaceutically acceptable carrier" may be a compound that is commonly used in formulation, does not suppress the activity of the active ingredient, is not toxic beyond the range that is applicable to the application or formulation target, and facilitates the addition of the compound into cells or tissues.

[0051] In some embodiments, the pharmaceutical composition may be formulated as a solid or liquid formulation. For example, when formulated as a solid formulation, it may further contain carriers, flavoring agents, binders, preservatives, disintegrants, lubricants, fillers, etc., and when formulated as a liquid formulation, it may further contain solutions such as water and propylene glycol solutions, suspensions, emulsions, etc., including, but not limited to, colorants, flavoring agents, stabilizers, viscosity enhancers, etc.

[0052] In embodiments, solid formulations may include powders, granules, tablets, capsules, suppositories, etc. For example, powders can be prepared by simply mixing the mekabu extract of the present invention, which is the active ingredient, with a carrier such as lactose, starch, or microcrystalline cellulose. Granules can be prepared by mixing the mekabu extract of the present invention with a carrier and a binder such as polyvinylpyrrolidone or hydroxypropyl cellulose, followed by a wet granulation method using a solvent such as water, ethanol, or isopropanol, or a dry granulation method using compression force. Tablets can also be prepared by mixing the granules with a lubricant such as magnesium stearate, followed by tableting using a tablet press.

[0053] In embodiments, the pharmaceutical composition may be administered orally, by injection (e.g., intramuscular injection, intraperitoneal injection, intravenous injection, infusion, subcutaneous injection, implant), inhalant, nasal administration, vaginal administration, rectal administration, sublingual administration, transdermal administration, topical administration, etc., depending on the disease or individual condition to be treated, but is not limited thereto. Furthermore, the pharmaceutical composition of the present invention may be formulated into an appropriate dosage unit form containing non-toxic, pharmaceutically acceptable carriers, additives, and vehicles commonly used depending on the administration route.

[0054] In the examples, the dosage of the pharmaceutical composition varies depending on the patient's condition and weight, the severity of the disease, the drug form, the administration route, and the duration of administration, but can be appropriately selected by those skilled in the art. The dosage form, administration route, and administration mode of the pharmaceutical composition may be independent of each other and are not particularly limited. Any administration route and administration mode may be used as long as the pharmaceutical composition can reach the target site. For example, the dosage form of the pharmaceutical composition according to the present invention may be used in the form of a pharmaceutically acceptable salt thereof, and may be used alone or in combination with other pharmaceutically active compounds, or in an appropriate combination. For example, the administration mode of the pharmaceutical composition according to the present invention may be, but is not limited to, intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration, or subcutaneous administration, as well as applying the composition to the diseased site, spraying, or inhaling the composition.

[0055] [Experimental Example] The following examples are provided to more specifically explain the present invention, but the present invention is not limited to these examples.

[0056] Experimental example 1: Confirmation of synergistic effects by using mixed oils (1) Preparation of Examples 1 to 3 Examples of compositions according to the present invention were prepared using the components listed in Table 1 below, where the unit of component content is % by weight.

[0057] [Table 1]

[0058] The specific manufacturing method is as follows. After mixing the oil components, the mixture was stirred uniformly for 10 minutes to complete the antibacterial compositions of Examples 1 to 3.

[0059] (2) Preparation of Comparative Examples 1 to 6 Comparative examples of compositions according to the present invention were prepared using the components listed in Table 2 below, in which the unit of component content is % by weight.

[0060] [Table 2]

[0061] The specific manufacturing method is the same as in Examples 1 to 3.

[0062] (3) Measurement of Minimal Inhibitory Concentration (MIC) The antibacterial activity of Example 1 and Comparative Example 1 was evaluated using Minimal Inhibitory Concentration (MIC), and the results are shown in Table 3 and FIG.

[0063] The experimental method was as follows. 100 μL of 200% TSB was added to a 96-well plate, and a sample was prepared to twice the oil concentration to be treated, and 100 μL of each was dispensed into the 96-well plate. The samples of Example 1 and Comparative Example 1 were then adjusted to 4000 ppm, 800 ppm, 160 ppm, and 32 ppm, respectively, and cultured at 32.5°C for 24 hours. After a certain period of time, the presence or absence of bacterial growth was determined by a color change (yellow indicates bacterial growth; colorless indicates bacterial growth inhibition). Control group 1 (C1) was a medium without inoculation of the bacterial strain, control group 2 (C2) was a medium without the addition of the antibacterial composition after inoculation of the bacterial strain, positive control group (C3) was a medium with phenoxyethanol added instead of the antibacterial composition, and negative control group (C4) was a medium with DMSO added.

[0064] [Table 3]

[0065] As a result of the experiment, in Example 1, in which three types of oils were mixed, the color appeared yellow at 800 ppm, but it appeared colorless at 4000 ppm. Therefore, it was found that the minimum inhibitory concentration of Example 1 against Staphylococcus aureus was 800 to 4000 ppm.

[0066] In addition, in the case of Comparative Example 1 containing only lemongrass oil, the color appeared yellow even at 4000 ppm, and it was therefore found that the minimum inhibitory concentration of Comparative Example 1 against Staphylococcus aureus exceeded 4000 ppm.

[0067] That is, it was confirmed that Example 1 had a stronger antibacterial activity against Staphylococcus aureus than Comparative Example 1.

[0068] (4) Paper Disk Diffusion Assay The antibacterial activity of Examples 1 to 3 and Comparative Examples 2 to 6 was evaluated using a Paper Disk Diffusion Assay, and the results are shown in Table 4.

[0069] The experimental method is as follows: Staphylococcus aureus strains were inoculated onto tryptic soy agar using a 100% loop and cultured for 24 hours. After that, the cultured strains were added to 0.85% NaCl at 1.0 x 10 7 The bacterial suspension was prepared by dispensing 100 μl of the prepared bacterial suspension into TSA medium, which was then smeared with glass beads to reduce the bacterial count to 1.0 × 10 6 The samples of the Examples and Comparative Examples were dispensed onto paper discs at 40 μl / disc, and after thorough absorption by the paper discs, they were placed on the culture medium inoculated with the bacterial strain. The sample-treated plates were then inverted and incubated at 32.5°C for 24 hours. After incubation, the short and long diameters of the inhibition zone around the disc were measured and the average value was calculated to evaluate the antibacterial activity.

[0070] [Table 4]

[0071] As a result of the experiment, in the cases of Examples 1 to 3, in which lemongrass oil, lavender oil and ylang-ylang oil were used in combination, the diameter of the growth inhibition zone was measured to be 43 mm or more in all cases, demonstrating excellent antibacterial activity.

[0072] In addition, in the case of Comparative Examples 2 and 3, which contained only one of lemongrass oil, lavender oil, and ylang-ylang oil, the diameter of the growth inhibition zone was measured to be less than 26 mm, and in the case of Comparative Examples 4 to 6, which contained only two of the above oils, the diameter of the growth inhibition zone was measured to be less than 38 mm, indicating that the antibacterial activity was lower than in Examples 1 to 3.

[0073] Experimental Example 2: Confirmation of the effect of oil weight ratio (1) Preparation of Comparative Examples 7 to 13 Examples of compositions according to the present invention were prepared using the components listed in Table 5 below, where the unit of component content is % by weight.

[0074] [Table 5]

[0075] The specific manufacturing method is the same as in Examples 1 to 3.

[0076] (2) Paper Disk Diffusion Assay The antibacterial activity of Examples 1 to 3 and Comparative Examples 7 to 13 was evaluated using the Paper Disk Diffusion Assay, which was the same experimental method as in Experimental Example 1. The results are shown in Table 6.

[0077] [Table 6]

[0078] As a result of the experiment, in the case of Examples 1 to 3, which contained lemongrass oil, lavender oil, and ylang-ylang oil in weight ratios within the range of the examples, the diameter of the growth inhibition zone was measured to be 43 mm or more in all cases, indicating that the antibacterial activity was very excellent.

[0079] In addition, in the case of Comparative Examples 7 to 13, which contained lemongrass oil, lavender oil, and ylang-ylang oil in weight ratios outside the range of the Examples, the diameter of the growth inhibition zone was measured to be less than 37 mm in all cases, indicating that the antibacterial activity was lower than in Examples 1 to 3.

[0080] Although certain aspects of the present invention have been described in detail above, those skilled in the art will recognize that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. Therefore, the true scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An antibacterial composition comprising: Contains Lemongrass Oil, Lavender Oil, and Ylang Ylang Oil, the weight ratio of the ylang-ylang oil, the lavender oil, and the lemongrass oil is 1:1.5-4.5:4-8; The composition has antibacterial activity against Staphylococcus strains. An antibacterial composition characterized by:

2. An antibacterial composition comprising the antibacterial composition of claim 1, and having the antibacterial activity of the antibacterial composition. A cosmetic composition characterized by:

3. An antibacterial composition comprising the antibacterial composition of claim 1, and having the antibacterial activity of the antibacterial composition. A skin external preparation composition characterized by:

4. An antibacterial composition comprising the antibacterial composition of claim 1, and having the antibacterial activity of the antibacterial composition. A pharmaceutical composition for preventing or treating hair loss, comprising:

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