Pharmaceutical compositions for the prevention or treatment of solid tumors, comprising epidithiodioxopiperazine derivatives or pharmaceutically acceptable salts thereof.

Abstract

The present invention relates to a pharmaceutical composition for preventing or treating solid cancers, which comprises, as an active ingredient, an epidithiodioxopiperazine derivative compound based on a core structure containing an intramolecular disulfide bond in the epidithiodioxopiperazine ring, or a pharmaceutically acceptable salt thereof.

Description

[Technical Field]

[0001] The present invention relates to a pharmaceutical composition for the prevention or treatment of solid cancer, comprising, as an active ingredient, an epidithiodioxopiperazine derivative compound or a pharmaceutically acceptable salt thereof, based on a parent structure containing an intramolecular disulfide bond in an epidithiodioxopiperazine ring. [Background technology]

[0002] Simply put, cancer is a general term for cells in the body that proliferate and grow faster than normal cells. Cancer is also called a tumor, but tumors are divided into benign tumors, in which normal cells simply grow and proliferate rapidly and increase in size to form a mass, and malignant tumors, in which abnormally deformed cells grow and / or proliferate and metastasize to other parts of the body. The term "cancer" in general refers to the aforementioned malignant tumor.

[0003] These types of cancers can be broadly divided into solid cancers and hematological cancers. Solid cancers, such as stomach cancer and lung cancer, originate in a specific organ, grow, and metastasize to other organs. In contrast, cancers that originate not in a specific organ but in hematopoietic organs that produce blood and immune cells are called hematological cancers because the cancer cells do not reside in a specific site but circulate in the blood and lymphatic system.

[0004] Solid tumors and hematological malignancies differ in their origin, location, and metastatic mechanisms, and therefore require different treatments. Solid tumors originate in specific organs, so surgery or radiation therapy is used when the tumor is limited to a particular organ, and chemotherapy is employed when these local treatments are ineffective. However, hematological malignancies are not limited to specific sites, so chemotherapy is the primary treatment regardless of the stage of disease progression. [Prior art documents] [Patent Documents]

[0005] [Patent Document 1] Korean Registered Patent Publication No. 10-1633975 [Non-patent literature]

[0006] [Non-Patent Document 1] Kirby and Robins, 1980, The Biosynthesis of Mycotoxins, New York: Academic Press [Non-Patent Document 2] Shah and Larsen, 1991, Mycopathologia, 116: 203-208 [Non-Patent Document 3] Sekita et al., 1981, Can. J. Microbiol., 27: 766-772 [Overview of the Initiative] [Problems that the invention aims to solve]

[0007] The inventors of this invention have diligently conducted research to find synthetic small molecule compounds derived from natural products and / or mimicking their functions for the treatment of solid tumors. As a result, they have confirmed that natural epidithiodioxopiperazine derivatives such as gliotoxin and ketomin, and synthetic small molecule derivatives that share a similar core structure, have activity to suppress the growth, proliferation, and / or metastasis of breast cancer, lung cancer, gastric cancer, skin cancer, colon cancer, prostate cancer, and pancreatic cancer, and have completed the present invention. [Means for solving the problem]

[0008] The present invention aims to provide a pharmaceutical composition for the prevention or treatment of solid cancers, comprising, as an active ingredient, an epidithiodioxopiperazine derivative compound or a pharmaceutically acceptable salt thereof, based on a parent structure containing an intramolecular disulfide bond in an epidithiodioxopiperazine ring. [Effects of the Invention]

[0009] The pharmaceutical compositions of the present invention, comprising natural and synthetic small molecule epidithiodioxopiperazine derivatives, not only efficiently kill breast cancer, lung cancer, gastric cancer, skin cancer, colon cancer, prostate cancer, and pancreatic cancer cells without affecting cell viability, but can also inhibit tumor growth and further reduce tumor volume in mouse models with transplanted tumors. [Brief explanation of the drawing]

[0010] [Figure 1] This figure shows (A) survival rate and (B and C) growth inhibitory effects of natural epidithiodioxopiperazine (ETP) compounds, gliotoxin, and chetomin on breast cancer cells at each treatment concentration of the compounds. [Figure 2] This figure shows the cytotoxic effects of synthetic epidithiodioxopiperazine derivatives on various cancer cells and normal cells. Figures A-J show the cell viability (n=3) obtained by treating breast cancer cells, lung cancer cells, gastric cancer cells, skin cancer cells, colon cancer cells, prostate cancer cells, pancreatic cancer cells, normal mammary cells, normal fibroblasts, and normal epithelial cells with the indicated ETP compound for 24 hours and quantifying them using the Cell titer-Glo assay system. The IC50 value was determined using a linear regression program (GraphPad Prism), and is shown in K. [Figure 3] This figure shows the inhibitory effect of gliotoxin on cell cycle progression in triple-negative breast cancer (TNBC) cells. [Figure 4] This figure shows the inhibitory effect of the natural epidithiodioxopiperazine compound, gliotoxin, on the migratory activity of breast cancer cells. [Figure 5] This figure shows the inhibitory effect of the natural epidithiodioxopiperazine compound, ketomin, on the metastatic activity of breast cancer cells. [Figure 6] It is a figure showing the inhibitory effect of a synthetic episulfidodioxopiperazine derivative on colony formation in breast cancer cells. [Figure 7] It is a figure showing the inhibitory effect of a synthetic episulfidodioxopiperazine derivative on colony formation in lung cancer cells. [Figure 8] It is a figure showing the inhibitory effect of a synthetic episulfidodioxopiperazine derivative on colony formation in gastric cancer cells. [Figure 9] It is a figure showing the inhibitory effect of a synthetic episulfidodioxopiperazine derivative on colony formation in skin cancer cells. [Figure 10] It is a figure showing the inhibitory effect of a synthetic episulfidodioxopiperazine derivative on colony formation in colon cancer cells. [Figure 11] It is a figure showing the inhibitory effect of a synthetic episulfidodioxopiperazine derivative on colony formation in prostate cancer cells. [Figure 12] It is a figure showing the inhibitory effect of a synthetic episulfidodioxopiperazine derivative on colony formation in pancreatic cancer cells. [Figure 13] It is a figure showing the inhibitory effect of a synthetic episulfidodioxopiperazine derivative on tumor growth in A549, SK-MEL-28, MKN-28 and Luc-MDA-MB-231 xenografts. A 100 mL vehicle control group (0.1% DMSO) and the indicated ETP compound (300 μg / kg) were intraperitoneally injected every three days for 20 days, and after 4 weeks, the xenografts were excised from the mice in each group. A to D show the tumor xenograft volumes and masses measured in A549, SK-MEL-28, MKN-28 and Luc-MDA-MB-231 xenograft mice for the control group (black), A2 (red), A5 (yellow-green) and A10 (yellow) treatments, respectively. The data in the graph are shown as mean ± S.E.M (n = 4, **P < 0.05). Statistical analysis was performed by one-way ANOVA with Tukey HSD post hoc test. [Figure 14]This figure shows the inhibitory effect of synthetic epidithiodioxopiperazine derivatives on cancer cell proliferation in vivo. A and C, and B and D show the proliferation index and immunohistochemistry determined by Ki-67 immunostaining of MDA-MB231-derived tumors and MKN28-derived tumors. The data in the graphs are the mean ± SD of the number of Ki-67+ cells per field in immunofluorescence staining (n=4, **P<0.001). E and F show the ETP-induced apoptosis incidence determined by TUNEL staining in MDA-MB231 and MKN-28-induced tumor xenografts, respectively. G is a sample treated with DNase, which is used as a positive control group in TUNEL staining. Specifically, tissue sections were treated with DNase I (10 U / mL) for 2 minutes. [Figure 15] This figure shows the inhibitory effect of synthetic epidithiodioxopiperazine derivatives on patient-derived lung adenocarcinoma transplanted into humanized mice. [Modes for carrying out the invention]

[0011] The present invention provides a pharmaceutical composition for the prevention or treatment of solid cancer, comprising, as an active ingredient, an epidithiodioxopiperazine derivative compound or a pharmaceutically acceptable salt thereof, based on a parent structure containing an intramolecular disulfide bond in an epidithiodioxopiperazine ring represented by chemical formula (1).

[0012] JPEG0007843697000001.jpg54170

[0013] The present invention is based on the discovery that a series of natural or synthetic small molecule compounds having an intramolecular disulfide bond in the epidithiodioxopiperazine ring exert therapeutic effects against solid cancer diseases due to the intramolecular disulfide bond contained therein. Specifically, the inventors have identified gliotoxin and ketomin, which are representative natural product-derived epidithiodioxopiperazine derivatives, as well as 5,7-dimethyl-2,3-dithia-5,7-diazabicyclo[2.2.2]octane-6,8-dione(A2) and 5,7-diallyl-2,3-dithia-5,7-diazabicyclo[2.2.2]octane-6,8-dione(A2), which are small molecule epidithiodioxopiperazine derivatives synthesized to confirm the core mechanism of action. Since we have confirmed that all of A5) and 5a,10a-dithio-octahydrodipyrrolo[1,2-a:1',2'-d]pyrazine-5,10-dione (A10) exhibit excellent therapeutic effects against solid tumors such as breast cancer, lung cancer, gastric cancer, skin cancer, colon cancer, prostate cancer, and pancreatic cancer, it is clear that epidithiodioxopiperazine derivatives containing intramolecular disulfide bonds will exhibit therapeutic effects against solid tumors regardless of their size or the type of substituents.

[0014] For example, the epidithiodioxopiperazine derivative compound contained in the pharmaceutical composition of the present invention may be an epidithiodioxopiperazine compound derived from a natural product represented by chemical formula (2) or (3), respectively.

[0015] JPEG0007843697000002.jpg47170

[0016] JPEG0007843697000003.jpg68170

[0017] For example, the compound of chemical formula (2) is a representative ETP compound called gliotoxin (GT), which is isolated from fungi such as Aspergillus fumigatus, Trichoderma virens, Penicillium species, and Candida albicans, as well as their culture media, metabolites, or secondary metabolites [Non-patent documents 1, 2].

[0018] The compound of chemical formula (3) is an ETP compound called chetomin, which is isolated from Chaetomium globosum [Non-patent Literature 3].

[0019] Furthermore, the epidithiodioxopiperazine derivative compound contained in the pharmaceutical composition of the present invention may be a synthetic small molecule compound represented by chemical formula (1-1).

[0020] JPEG0007843697000004.jpg50170

[0021] In chemical formula (1-1), R1 to R4 are each independently hydrogen and C 1-6 Linear or branched alkyl, alkenyl, alkynyl, C 1-6 Alkoxy-aryl-C 1-6 Alkyl or 5-10 member heteroaryl-C 1-6 Either they are alkyl groups, or R1 and R2, and R3 and R4 are independently linked to each other, forming a 4- to 10-membered heterocycle containing the bonded carbon and nitrogen atoms.

[0022] Specifically, the compound represented by chemical formula (1-1) is a synthetic small molecule compound represented by chemical formulas (4) to (14), but is not limited to these.

[0023] JPEG0007843697000005.jpg48170

[0024] JPEG0007843697000006.jpg52170

[0025] JPEG0007843697000007.jpg48170

[0026] JPEG0007843697000008.jpg48170

[0027] JPEG0007843697000009.jpg50170

[0028] JPEG0007843697000010.jpg54170

[0029] JPEG0007843697000011.jpg50170

[0030] JPEG0007843697000012.jpg50170

[0031] JPEG0007843697000013.jpg51170

[0032] JPEG0007843697000014.jpg53170

[0033] JPEG0007843697000015.jpg49170

[0034] For example, the epidithiodioxopiperazine derivative compound contained in the pharmaceutical composition of the present invention may be a compound of chemical formula (2), (3), (5), (7), or (14).

[0035] JPEG0007843697000016.jpg50170

[0036] JPEG0007843697000017.jpg70170

[0037] JPEG0007843697000018.jpg50170

[0038] JPEG0007843697000019.jpg51170

[0039] JPEG0007843697000020.jpg52170

[0040] The derivative of the epidithiodioxopiperazine compound refers to a compound containing an epidithiodioxopiperazine ring as the active core. The derivative may be a compound in which the NH group or CH group of the ring of the compound represented by chemical formula (4) is substituted with various substituents of a type known in the art, or it may be a compound having a structure formed by bonding various compounds that are obvious to the ordinary person in the art, but is not limited to these. Modification and substitution of the compound structure of chemical formula (4) can be easily carried out by a person skilled in the art, for example, by the following process.

[0041] JPEG0007843697000021.jpg51170

[0042] The compounds of chemical formulas (4) to (14) can be used by those skilled in the art by synthesis with reference to known methods. For specific synthesis methods, please refer to the method disclosed in Patent Document 1.

[0043] The compositions of the present invention can achieve preventive or therapeutic effects against solid tumors by mimicking the intracellular activity of PrxII, but the specific mechanism of action is not limited thereto. For example, epidithiodioxopiperazine derivative compounds included in the compositions of the present invention can mimic the activity of PrxII, and can therefore target cancer cells with low or no peroxiredoxin expression to exhibit anticancer activity, but are not limited thereto.

[0044] For example, solid cancers that can be prevented or treated using the composition of the present invention include, but are not limited to, breast cancer, lung cancer, gastric cancer, skin cancer, colon cancer, prostate cancer, or pancreatic cancer.

[0045] In this invention, "pharmaceutically acceptable salt" means any salt that has the desired biological and / or physiological activity of the compound or derivative and minimizes undesirable toxicological effects. In this invention, any type of salt is acceptable as long as it retains a diketopiperazine ring containing an intramolecular disulfide bond. As salts, acid addition salts formed with a pharmaceutically acceptable free acid are useful. Acid addition salts are prepared by conventional methods, for example, by dissolving the compound in an excess amount of aqueous acid solution and precipitating the salt using a water-miscible organic solvent, such as methanol, ethanol, acetone, or acetonitrile. Alternatively, equimolar amounts of the compound and an acid or alcohol in water (e.g., glycol monomethyl ether) may be heated, and then the mixture may be evaporated and dried, or the precipitated salt may be filtered by suction. Here, inorganic acids and organic acids can be used as free acids. Inorganic acids include hydrochloric acid, hydrobromic acid, phosphoric acid, nitric acid, and sulfuric acid. Organic acids include methanesulfonic acid, p-toluenesulfonic acid, acetic acid, trifluoroacetic acid, maleic acid, succinic acid, oxalic acid, benzoic acid, tartaric acid, fumaric acid, mandelic acid, propionic acid, citric acid, lactic acid, glycolic acid, gluconic acid, galacturonic acid, glutamic acid, glutaric acid, glucuronic acid, aspartic acid, ascorbic acid, carboxylic acids, vanillic acid, and hydroiodic acid, but are not limited to these.

[0046] Furthermore, pharmaceutically acceptable metal salts can be formed using a base. Alkali metal or alkaline earth metal salts can be obtained, for example, by dissolving a compound in an excess amount of alkali metal hydroxide or alkaline earth metal hydroxide solution, filtering out the undissolved salts of the compound, and evaporating and drying the filtrate. Here, sodium, potassium, or calcium salts are particularly preferred as metal salts from a pharmaceutical standpoint, but are not limited to these. Corresponding silver salts can be obtained by reacting an alkali metal or alkaline earth metal salt with a suitable silver salt (e.g., silver nitrate).

[0047] Unless otherwise specified, the pharmaceutically acceptable salts of epidithiodioxopiperazine compounds or derivatives thereof according to the present invention include all possible salts of acidic or basic groups. For example, pharmaceutically acceptable salts include sodium, calcium, and potassium salts of the hydroxyl group, and other pharmaceutically acceptable salts of the amino group include hydrobromide, sulfate, hydrogen sulfate, phosphate, hydrogen phosphate, dihydrogen phosphate, acetate, succinate, citrate, tartrate, lactate, mandelate, methanesulfonate (mesylate), and p-toluenesulfonate (tosylate), which can be produced by salt production methods known in the art.

[0048] The composition according to the present invention may further comprise suitable carriers, excipients, and diluents commonly used in the manufacture of pharmaceutical compositions. The composition is sterile or aseptic, and its solution is also sterile or aseptic, and may comprise water, buffers, isotonic agents, or other components known to those with ordinary skill in the art that do not cause allergic or other harmful reactions when applied to animals or humans.

[0049] In this invention, "pharmaceutically acceptable carriers" include any solvent, dispersion medium, coating agent, antimicrobial agent, antifungal agent, isotonic agent, etc. The use of the above media and formulations for pharmaceutically active substances is known in the art. In addition to ordinary media or formulations that are immiscible with the active ingredient, their use in therapeutic compositions is also conceivable. Furthermore, supplemental active ingredients may be mixed into such compositions.

[0050] The composition may be manufactured in dosage forms such as liquids, emulsions, suspensions, and creams, and may be administered parenterally. The dosage of the composition can be the usual dosage for preventing vascular restenosis, and it is preferable that different dosages be applied depending on the patient's age, sex, health condition, the degree of absorption and inactivation of the active ingredient in the body, and any drugs used in combination.

[0051] Furthermore, the present invention provides a method for preventing or treating solid cancer, comprising the step of administering the pharmaceutical composition to an individual requiring the pharmaceutical composition.

[0052] In the present invention, "prevention" means any action that suppresses or delays the onset of solid cancer by administering the pharmaceutical composition according to the present invention, and "treatment" means any action that improves or favorably alters the symptoms caused by solid cancer by administering the pharmaceutical composition.

[0053] In this invention, "individual" refers to any animal, including humans, monkeys, cattle, horses, sheep, pigs, chickens, turkeys, quail, cattle, dogs, mice, rats, rabbits, guinea pigs, etc., that has developed or is at risk of developing solid cancer. By administering the pharmaceutical composition of this invention to an individual, the aforementioned disease can be effectively prevented or treated. Furthermore, the pharmaceutical composition of this invention may be administered in parallel with known solid cancer treatment agents.

[0054] The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. The “pharmaceutically effective amount” means an amount sufficient to treat the disease with a reasonable benefit / risk ratio applicable to medical treatment, without causing side effects. The effective dose level can be easily determined by a person skilled in the art based on the patient’s sex, age, weight, health status, disease severity, drug activity, sensitivity to the drug, method of administration, time of administration, route of administration and elimination rate, duration of treatment, drugs used in combination or concurrently, and other factors known in the medical field.

[0055] In the present invention, "administration" means providing a predetermined substance to a patient by any suitable method, and the administration route of the composition of the present invention may be any common route that can deliver it to the target tissue. Examples include, but are not limited to, intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, local administration, intranasal administration, intrapulmonary administration, and rectal administration. Furthermore, the pharmaceutical composition of the present invention can be administered by any device capable of delivering the active substance to target cells. Preferred administration methods and formulations include intravenous injection, subcutaneous injection, intradermal injection, intramuscular injection, and intravenous infusion. The injectable preparation can be prepared using aqueous solvents such as physiological saline solution and Ringer's solution, or non-aqueous solvents such as vegetable oil, higher fatty acid esters (e.g., ethyl oleate), and alcohols (e.g., ethanol, benzyl alcohol, propylene glycol, glycerin). It may also contain pharmaceutical carriers such as stabilizers to prevent deterioration (e.g., ascorbic acid, sodium bisulfite, sodium pyrosulfite, BHA, tocopherol, EDTA), emulsifiers, buffers to adjust pH, and preservatives to inhibit microbial growth (e.g., phenylmercury nitrate, thimerosal, benzalkonium chloride, phenol, cresol, benzyl alcohol).

[0056] The pharmaceutical composition of the present invention may further contain, as an active ingredient, a known drug used for the prevention or treatment of known solid tumors, other than an epidithiodioxopiperazine derivative compound or a pharmaceutically acceptable salt thereof, such as a known anticancer agent, for the prevention or treatment of solid tumors. Furthermore, it may be used in combination with other known treatments for the treatment of these diseases. Other treatments include, but are not limited to, chemotherapy, radiotherapy, hormone therapy, bone marrow transplantation, stem cell therapy, other biological treatments, and immunotherapy.

[0057] For example, the aforementioned cancers include lung cancer, small cell lung cancer, non-small cell lung cancer, colorectal cancer, pancreatic cancer, stomach cancer, liver cancer, breast cancer, cervical cancer, thyroid cancer, parathyroid cancer, prostate cancer, gallbladder cancer, biliary tract cancer, non-Hodgkin lymphoma, Hodgkin lymphoma, bladder cancer, kidney cancer, ovarian cancer, melanoma, colon cancer, bone cancer, skin cancer, head cancer, uterine cancer, rectal cancer, brain tumor, perianal gland cancer, fallopian tube cancer, endometrial cancer, vaginal cancer, vulvar cancer, esophageal cancer, small intestine cancer, endocrine gland cancer, adrenal cancer, soft tissue sarcoma, urethral cancer, penile cancer, ureteral cancer, renal cell carcinoma, renal pelvis cancer, central nervous system tumors, primary CNS lymphoma, spinal cord tumors, brainstem glioma, pituitary adenoma, glioma, gliosarcoma, anaplastic astrocytoma, medulloblastoma, cervical This may include carcinoma, chordoma, pharyngeal carcinoma, Kaposi's sarcoma, lymphangiosarcoma, intralymphatic sarcoma, colorectal carcinoma, cholangiocarcinoma, choriocarcinoma, seminomas, testicular tumor, Wilms' tumor, Ewing's tumor, angiosarcoma, endosarcoma, adenocarcinoma, sweat gland carcinoma, sebaceous gland sarcoma, papillary sarcoma, papillary adenocarcinoma, cystic adenosarcoma, bronchial carcinoma, medullary carcinoma, mast cell tumor, mesothelioma, synoviomas, leiomyosarcoma, rhabdomyosarcoma, neuroblastoma, retinoblastoma, oligodendroglioma, acoustic neuroma, angioblastoma, meningioma, pineal cell tumor, ependymoma, craniopharyngioma, epithelial carcinoma, embryonic carcinoma, squamous cell carcinoma, basal cell carcinoma, fibrosarcoma, myxoma, myxosarcoma, liposarcoma, chondrosarcoma, osteosarcoma, or metastatic cancers thereof. [Examples]

[0058] The present invention will be described in more detail below with reference to examples. These examples are merely for the purpose of illustrating the present invention in more detail, and the present invention is not limited to these examples.

[0059] Experimental Example 1: Cell line and culture conditions From the American Type Culture Collection (ATCC), we obtained breast cancer cell lines MDA-MB-231 and Hs-578T, lung cancer cell lines A549 and H460, gastric cancer cell lines MKN28 and MKN74, skin cancer cell lines SK-MEL-5 and SK-MEL-28, colon cancer cell lines RKO and HCT116, prostate cancer cell line PC3, pancreatic cancer cell line Panc-1, normal breast cell line MCF-10A, normal lung fibrous cell line IMR90, and normal colon cell line CCD-841. Hs-578T, H460, MKN28, and MKN74 cells were cultured in high-glucose RPMI 1640 supplemented with 10% fetal bovine serum (FBS, Hyclone), 100 U / mL penicillin, and 100 U / mL streptomycin. MDA-MB-231, A549, PC3, and Panc-1 cells were cultured in high-glucose DMEM (Dulbecco's Modified Eagle Medium) supplemented with 10% FBS, 100 U / mL penicillin, and 100 U / mL streptomycin. SK-MEL-5 and SK-MEL-28 cells were cultured in high-concentration glucose EMEM (Eagle's Minimum Essential Medium) supplemented with 10% FBS, 100 U / mL penicillin, and 100 U / mL streptomycin. IMR90 and CCD-841 cells were cultured in high-concentration glucose MEM (Minimum Essential Medium) supplemented with 10% FBS, 100 U / mL penicillin, and 100 U / mL streptomycin. RKO cells were cultured in high-concentration glucose McCoy supplemented with 10% FBS, 100 U / mL penicillin, and 100 U / mL streptomycin. MCF-10A cells were cultured in DMEM F12 supplemented with 10% FBS, 100 U / mL penicillin, and 100 U / mL streptomycin. These cells were subcultured in a 5% CO2 atmosphere at 37°C.

[0060] Experimental Example 2: Cell Proliferation Assay Cells (2×10 3 cells / well) were cultured in a 96-well plate for 24 hours and then treated with the compound of the present invention for 2 hours. The cells were seeded in fresh complete medium and cultured for the indicated time. Cell proliferation was determined by treating the cells with WST-1 reagent for 1 hour and measuring the absorbance of the formazan product at 450 nm using a UV / VIS ELISA reader.

[0061] Experimental Example 3: Cell Viability Assay To determine the IC 50 value capable of confirming cytotoxicity, cells (2×10 5 cells / well) were dispensed into a 96-well plate and treated with the indicated compound at various concentrations for 24 hours. Cell viability was measured using the CellTiter-Glo Luminescent Assay Reagent Kit (Promega, G7570), and luminescence was read using an Envision plate reader. The percentage of cell growth relative to the vehicle control group (cells treated with 0.1% DMSO) was calculated.

[0062] Experimental Example 4: Cell Cycle Assay Cells (2×10 5 cells / well) were cultured in a 6-well plate for 24 hours and then treated with the compound of the present invention for 2 hours. The cells were seeded in fresh complete medium and cultured for an additional 24 hours. The cells were fixed overnight at 4°C in 70% ethanol, washed with cold PBS, and then re-suspended in a PBS solution containing RNase A (10 μg / mL) at 37°C for 30 minutes. The above-mentioned suspended cells were stained with propidium iodide (PI, 50 μg / mL, Invitrogen) for 5 minutes, and flow cytometry (FACS Calibur, BD) was performed. The cell population was analyzed using ModiFit LT software.

[0063] Experimental Example 5: Metastasis Assay Cells (2×10 5Cells (5 × 10⁶ cells / well) were cultured in a 6-well plate for 24 hours, and then treated with the compound of the present invention for 2 hours. After treatment, the cells were cultured for a further 12 hours in fresh culture medium containing 0.5% FBS to make them serum-starved. Serum-starved cells (5 × 10⁶ cells / well) were placed in the upper chamber (8 μm pore size) of a 24-well transwell culture plate (Costar) pre-coated with 0.1% gelatin B (Sigma Aldrich) at the bottom. 4 Cells were transferred (per well). The lower chamber was filled with culture medium containing 20% ​​FBS. 24 hours after plating, non-migrated cells in the upper chamber were removed. After removing the non-migrated cells from the upper chamber, the upper chamber was fixed with methanol and stained with 0.6% hematoxylin (DAKO). Photographs of the stained cells were taken and counted. The number of migratory cells was averaged across the three wells.

[0064] Experimental Example 6: Colony Formation Assay cells (2×10 3 Cells were cultured in a 6-well plate for 24 hours, and then treated with the compound of the present invention for 2 hours. The cells were seeded in fresh complete medium and cultured for a further 10 days. The cells were washed with PBS and stained with crystal violet. The blue-stained colonies were manually counted.

[0065] Experimental Example 7: Mouse Xenograft Assay A549, SK-MEL-28, MKN-28, and Luc-MDA-MB-231 cells (2 × 10⁻¹⁰ 6Cells were suspended in 100 μL of Matrigel solution (BD Biosciences) and subcutaneously injected into 5-week-old male mice. Seven days later, the mice were intraperitoneally injected with 100 μL of vehicle control (0.1% DMSO) and the compound (300 μg / kg) every three days for 20 days. In tumors induced by A549, SK-MEL28, and MKN28 cells, the tumor volume was calculated using the following formula by caliper measurement of the width (W) and length (L) of the xenografted tumors.

[0066] (Math 1) Volume = [L × W] 2 ] / 2

[0067] In tumors induced by Luc-MDA-MB-231 cells, tumor volume was measured by 2D optical topography. Briefly, mice were intraperitoneally injected with D-luciferin solution (150 mg / kg, Caliper Life Sciences), anesthetized with 2% isoflurane 10 minutes later, and then a 10-second video was captured using the IVIS 200 system (Xenogen).

[0068] Experimental Example 8: Immunohistochemistry and Immunofluorescence Tumor tissue samples embedded in paraffin blocks were cut into 4 μm thick sections. The tissue sections were deparaffinized according to a standard protocol and rehydrated in a series of graded alcohols. Antigens were immersed in antigen unmasking solution (VECTOR, H-3300-250) at 95°C for 15 minutes, and washed with PBS after the temperature cooled to room temperature. Endogenous peroxidase activity was reduced with BLOXALL® Endogenous Blocking Solution and incubated for 30 minutes. The tissue sections were blocked for 1 hour with a blocking solution containing 0.1% Triton X-100 and normal goat serum, and then reacted with anti-Ki67 antibody (1:100, Invitrogen, PA1-38032) at room temperature for 2 hours. For immunohistochemistry (IHC), slides were washed three times with PBS and incubated with a biotinylated secondary antibody (1:200) using 0.1% Triton X-100 for 1 hour at room temperature. ImmPACT® DAB substrate (VECTOR, SK-4105) was added to the slides to represent the fluorescent color. For immunofluorescence (IF), the washed slides were incubated with a fluorescent secondary antibody (1:200) conjugated with 0.1% Triton X-100 for 1 hour. The fluorescence was visualized and imaged using a confocal microscope (Nikon A1R).

[0069] Experimental Example 9: TUNEL Assay Luc-MDA-MB-231 and MKN-28 xenograft tumor samples were surgically separated, then frozen-embedded in an OCT (optimal cutting temperature) compound solution on dry ice for 10 minutes, and subsequently cut into 10 μm thick sections. The tissue sections were washed with distilled water for 10 minutes and fixed in 4% formalin for 20 minutes. The tissue sections were then washed with PBS for 30 minutes and incubated in permeabilization solution (0.1% Triton X-100) for 5 minutes. Apoptotic cells were visualized using an In situ Cell Death Detection Kit (Roche, 11684795910) according to the manufacturer's instructions. Cell images were obtained using a microscope (Zeiss LSM880 Airyscan).

[0070] Experimental Example 10: Xenograft Experiment of Patient-Derived Tumors The animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of Seoul National University College of Medicine and followed the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines. Patient-derived lung tumors were treated with NSG (NOD-scid-IL2Rg) at 4 weeks and 17 weeks of age. null The tumors were subcutaneously implanted into the backs of ) and humanized NSG (HuNSG) mice, respectively. Compound (A5, 300 mg / kg) of chemical formula (7) dissolved in PBS and / or pembrolizumab (humanized anti-PD-1 antibody, 5 mg / kg) were intraperitoneally injected into the mice every 3 days for 3 weeks and every 5 days for 3 weeks, respectively. The tumors were allowed to grow for 28 days, then removed and weighed. The tumor volume was measured every 3 days using Vernier calipers and calculated using the following formula.

[0071] (Math 2) V = a × b 2 / 2

[0072] In the above formula, a and b represent the superficial diameters of the major and minor axes, respectively. [Examples]

[0073] Cytotoxic and cancer cell proliferation inhibitory effects of natural epidithiodioxopiperazine derivatives In Experimental Example 1, breast cancer cell lines MDA-MB-231 and Hs-578T were prepared and treated with DMSO and gliotoxin, a natural epidithiodioxopiperazine derivative, as control vehicles. As in Experimental Example 2, the cytotoxicity and proliferation rate at each concentration of the treatment compound were calculated. The results are shown in Figure 1.

[0074] As shown in Figure 1A, treatment with gliotoxin and ketomin resulted in a high cell viability rate of over 80% up to a concentration of approximately 200 nM. This suggests that these compounds are not cytotoxic at this concentration; therefore, in the experiments to confirm the following pharmacological activity, concentrations within this range were selectively used.

[0075] On the other hand, as shown in Figures 1B and 1C, the proliferation rate was significantly reduced in cells treated with gliotoxin (100 nM) or ketomin (50 nM) compared to the control group of DMSO-treated cells. This indicates that natural epidithiodioxopiperazine derivatives effectively suppress cancer cell proliferation. [Examples]

[0076] Inhibitory effect of natural epidithiodioxopiperazine derivatives on the cell cycle progression of cancer cells In Experimental Example 1, breast cancer cell lines MDA-MB-231 and Hs-578T were prepared, and either treated with or left untreated with gliotoxin, a natural epidithiodioxopiperazine derivative, or their cell cycle was analyzed as in Experimental Example 4, and the percentage of cells in the G1 phase was calculated. The results are shown in Figure. 3 This will be shown.

[0077] figure 3 As shown, it was confirmed that when gliotoxin was added to the culture, the percentage of cells in the G1 phase increased by more than 10% compared to the untreated group. [Examples]

[0078] Inhibitory effect of natural epidithiodioxopiperazine derivatives on the metastatic activity of cancer cells In Experimental Example 1, breast cancer cell lines MDA-MB-231 and Hs-578T were prepared and treated with DMSO and gliotoxin (a natural epidithiodioxopiperazine derivative) as control vehicles. As in Experimental Example 5, cell migration to FBS-containing medium was observed, and the number of migrating cells was counted. The results are shown in Figure. 4 and figure 5 This will be shown.

[0079] figure 4 and figure 5 As shown, treatment with gliotoxin and ketomin significantly reduced the migration of breast cancer cells into serum-containing media. [Examples]

[0080] Selective cancer cell death effect of synthetic epidithiodioxopiperazine derivatives The cytotoxic effects of the synthetic epidithiodioxopiperazine derivative of the present invention on various cancer cells and normal cells were confirmed. The results are shown in the figure. 2 As shown in the figure. 2 As shown, the synthetic epidithiodioxopiperazine derivative of the present invention exhibits significantly lower IC50 in various cancer cell lines compared to three types of normal cells (MCF-10A, IMR90, CCD-841). 50The value was shown. This indicates that it selectively kills cancer cells, which are not normal cells. [Examples]

[0081] Inhibitory effect of synthetic epidithiodioxopiperazine derivatives on cancer cell colony formation Of the cancer cells prepared in Experimental Example 1, the breast cancer cell lines MDA-MB-231 and Hs-578T, the lung cancer cell lines A549 and H460, the gastric cancer cell lines MKN28 and MKN74, the skin cancer cell lines SK-MEL-5 and SK-MEL-28, the colon cancer cell lines RKO and HCT116, the prostate cancer cell line PC3, and the pancreatic cancer cell line Panc-1 were treated with DMSO as a control vehicle and synthetic epidithiodioxopiperazine derivatives A2, A5, and A10. As in Experimental Example 6, the cell-killing activity of the epidithiodioxopiperazine derivatives against these cancer cells was observed visually, and the number of formed colonies was counted. The results are shown in Figures 6 to 12.

[0082] As shown in Figures 6 to 12, colony formation of these cancer cells was efficiently inhibited at low concentrations (50 nM and 200 nM) that did not exhibit nonspecific cytotoxicity, although to varying degrees, for all of A2, A5, and A10. For example, compound A10 inhibited colony formation in most cancer cells even at a concentration of 50 nM, while A2 and A5 showed colony formation inhibition effects equivalent to or slightly better than the control vehicle-treated group when treated at a concentration of 50 nM, and when the treatment concentration was increased to 200 nM, they showed remarkably superior colony formation inhibition effects that were visible to the naked eye across all tested cells. [Examples]

[0083] Growth inhibitory effect of synthetic epidithiodioxopiperazine derivatives on xenografts In a mouse xenograft model prepared by injecting various cancer cell lines prepared in Experimental Example 7, the tumor growth inhibitory effect of treatment with the synthetic epidithiodioxopiperazine derivative of the present invention was confirmed. The results are shown in Figure 13. Specifically, the compound of the present invention was administered for 30 days, and the tumor size was measured. On the final day, the 30th day, the mice were sacrificed, and the tumors were excised and their volume and weight were measured. As a control group, mice administered DMSO as a vehicle instead of the compound of the present invention were used. As shown in Figure 13, treatment with the compound of the present invention significantly inhibited the growth of all xenograft tumors using lung cancer, skin cancer, gastric cancer, and breast cancer cell lines, and the size of the formed tumors was remarkably smaller than that of the control group. This indicates that the compound of the present invention effectively inhibits tumor growth. [Examples]

[0084] Inhibitory effect of synthetic epidithiodioxopiperazine derivatives on the proliferation of cancer cells in vivo. The inhibitory effect of synthetic epidithiodioxopiperazine derivatives on in vivo cancer cell proliferation was confirmed using immunohistochemistry, immunofluorescence, and TUNEL assays in Experimental Examples 8 and 9. The results are shown in Figure 1. 14 As shown in the figure. 14 As shown, treatment with the compound of the present invention significantly reduced the expression of Ki-67, which is involved in tumor proliferation, in both breast cancer and gastric cancer cell lines compared to the control group. In contrast, when using TUNEL staining to measure cancer cell death, no cell death was observed in either the vehicle control group or the groups treated with the compound of the present invention. The accuracy of these TUNEL assays was verified by artificially inducing DNA damage by treating tissue sections with DNase (Figure). 14 (G) These suggest that the compounds of the present invention exert preventive or therapeutic effects against cancer by effectively inhibiting the proliferation of cancer cells, rather than by killing them. [Examples]

[0085] Tumor growth inhibitory effect of synthetic epidithiodioxopiperazine derivatives Mouse models prepared by transplanting patient-derived lung adenocarcinoma tumors, as prepared in Experimental Example 10, were treated with A5, a representative synthetic epidithiodioxopiperazine derivative, either without treatment, alone, or in combination with pembrolizumab, a humanized antibody known to be used in cancer immunotherapy. The volume changes of the tumors were observed periodically. The results are shown in Figure 15.

[0086] As shown in Figure 15, in the untreated control group of NSG mice, tumor volume increased over time, whereas in the A5-treated group, tumor volume increase was suppressed. Administration of A5 to humanized NSG mice (HuNSG) not only reduced tumor size compared to the untreated control group, but also showed superior effects in suppressing tumor growth and further reducing tumor size in the A5 monotherapy group compared to the pembrolizumab-treated group and the experimental group treated with pembrolizumab in combination.

[0087] From the above description, those skilled in the art in which the present invention pertains will understand that the present invention can be implemented in other specific forms without altering its technical idea or essential features. It should be understood that the above embodiments are merely illustrative and not limiting. The present invention should be interpreted as including all modifications and variations derived from the meaning and scope of the claims and their equivalent concepts, rather than the specification.

Claims

1. A pharmaceutical composition for the prevention or treatment of solid cancer, comprising, as an active ingredient, an epidithiodioxopiperazine derivative compound or a pharmaceutically acceptable salt thereof, based on a parent structure containing an intramolecular disulfide bond in an epidithiodioxopiperazine ring represented by the following chemical formula (1-1), The composition wherein the solid tumor is breast cancer, lung cancer, gastric cancer, prostate cancer, or pancreatic cancer. 【Chemistry 1】 (In the formula, R1 to R4 are each independently hydrogen, methyl, or allyl, or R1 and R2, and R3 and R4 are each independently linked to one another, forming a five-membered heterocycle containing the carbon and nitrogen atoms they bond to.

2. The composition according to claim 1, wherein the compound represented by the chemical formula (1-1) is at least one selected from the compounds of chemical formulas (4) to (7), (10), and (14). 【Chemistry 2】 【Transformation 3】 【Chemistry 4】 【Transformation 5】 【Transformation 6】 【Transformation 7】

3. The composition according to claim 1, wherein the epidithiodioxopiperazine derivative compound is a compound of chemical formula (5), (7), or (14). 【Transformation 8】 【Chemistry 9】 【Chemistry 10】

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