Culture medium, method for inducing differentiation of adipose-derived stem cells into myoblasts

Abstract

This invention provides a culture medium that promotes Pax7 expression from mesenchymal stem cells and effectively induces differentiation into myoblasts. [Solution] A culture medium comprising serum, an IMDM (Iscove's Modified Dulbecco's Medium) medium in an amount between 2 and 8 times the volume of serum, a basic medium in an amount between 2.5 and 10 times the volume of serum, dexamethasone in a concentration of 0.25 mM to 1 mM, and insulin in a concentration of 10 ng / mL to 50 ng / mL.

Claims

1. A basic medium comprising serum, IMDM (Iscove's Modified Dulbecco's Medium) medium in an amount of 2 to 8 times the volume of the serum, and Ham's F-12 (Ham's Nutrient Mixture F-12) medium in an amount of 2.5 to 10 times the volume of the serum, Dexamethasone with a concentration of 0.25 mM or more and 1 mM or less, A culture medium for inducing the differentiation of mesenchymal stem cells into myoblasts, containing insulin at a concentration of 10 ng / mL to 50 ng / mL.

2. A culture medium according to claim 1, The basic culture medium is a culture medium comprising IMDM medium in an amount of 3 to 6 times the volume of the serum and Ham's F-12 medium in an amount of 4 to 7 times the volume of the serum.

3. A method for producing myoblasts, which includes a differentiation induction step in which mesenchymal stem cells are differentiated into myoblasts, The differentiation induction step includes a resting step, which is a step of suspending mesenchymal stem cells in the medium described in claim 1 and culturing them in a static environment at 4°C or higher and 10°C or lower for 1 day or more and 4 days or less to obtain a culture.

4. The method according to claim 3, The differentiation induction step further comprises a post-culturing step, which, after the standing step, involves seeding the culture into a culture vessel and culturing it in serum medium or the medium described in claim 1 for 4 days to 2 weeks.

5. The method according to claim 4, A method comprising the above-mentioned standing step and the above-mentioned post-culturing step, wherein the expression of Pax7 and muscle-specific gene group MRFs is induced.

6. A method according to claim 5, wherein the muscle-specific gene group MRFs comprises Myf5, MyoD, Myogenin, and MRF4.

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