CYS-MAB Conjugation Method

Abstract

To provide robust ways to manufacture conjugates of cysteine-engineered antibodies that offer a high yield and consistent product quality.SOLUTION: A method comprises the steps of; a) obtaining a composition comprising an antibody or antibody fragment; b) exposing the antibody or antibody fragment to a cysteine blocking agent, where the cysteine blocking agent forms a stable mixed-disulfide with at least one cysteine residue of the antibody or antibody fragment; c) adding a reducing agent to the composition to form a reduction mix and allowing a reduction reaction to occur such that the reduction mix comprises a reduced antibody or reduced antibody fragment; d) adding an oxidizing agent to the reduction mix to form an oxidized mix and allowing an oxidizing reaction to occur such that the oxidizing mix comprises an oxidized antibody or oxidized antibody fragment; and e) adding an activated chemical moiety to the oxidized mix to form a conjugation mix and allowing a conjugation reaction to occur such that an antibody conjugate or antibody fragment conjugate is formed.SELECTED DRAWING: Figure 6

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