Collection kit and collection method

Abstract

To provide a collection kit and a collection method for efficient culture testing of blood product.SOLUTION: A collection kit 10 comprises: an inflow tube 12 to which a medical bag is connected; a storage section 14 having a first storage chamber 31 and a second storage chamber 32; an air vent 16 for discharging air from the storage section 14; and at least one adapter 21, 22 connectable to culture bottles B1, B2. A communication path 33 is formed in the upper part of the storage section 14 to communicate the first storage chamber 31 and the second storage chamber 32. A hydrophilic filter 24 is arranged in a transfer pipe 38 of the adapters 21, 22. In the collection method, an object to be collected is collected into two culture bottles B1 and B2 using the collection kit 10.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to a collection kit and a collection method for transferring a collection target to a culture bottle.

Background Art

[0002] Blood products include red blood cell products, plasma products, platelet products, and whole blood products. Blood products are preparations containing components required by patients and are used for transfusion. Blood products are stored and transported in a state of being contained in a medical bag. In order to ensure safety, a small amount of sample may be collected for a culture test. The culture test detects the presence or absence of pathogens by collecting the sample in a culture bottle and placing the culture bottle in an environment suitable for the growth of bacteria.

[0003] For example, the test of platelet products is performed according to the following procedure. A small-volume collection bag is connected to a medical bag (hereinafter referred to as a platelet bag) containing platelet products. Then, a part of the platelet products in the platelet bag is transferred to the collection bag. Next, the collection bag is detached from the platelet bag.

[0004] Next, the collection bag is carried to a clean bench. In the clean bench, the platelet products are injected from the collection bag into a blood culture bottle. Prior to the injection, the tube of the collection bag is connected to a tube extending from a sample collection tube. Based on the scale of the sample collection tube as a reference, a prescribed amount of platelet products is transferred from the collection bag to the sample collection tube.

[0005] After that, while visually observing the scale of the sample collection tube, the operator operates the valve of the sample collection tube to transfer a prescribed amount of platelet products from the sample collection tube to the blood culture bottle. In the culture test, anaerobic culture and aerobic culture are performed. Therefore, the platelet products are dispensed into a culture bottle used for anaerobic culture and a culture bottle used for aerobic culture. Then, each culture bottle is set in a culture device and subjected to a culture test for a predetermined period.

[0006] For example, Patent Document 1 discloses a sample collection kit for collecting samples from a medical bag. [Prior art documents] [Patent Documents]

[0007] [Patent Document 1] U.S. Patent No. 8777921 [Overview of the project] [Problems that the invention aims to solve]

[0008] When performing large-scale culture tests on blood products, improving the efficiency of the culture testing process is essential. Conventional testing procedures, as described above, require the removal and reattachment of collection bags and sample collection tubes during the process of dispensing blood products from the blood product bag into culture bottles, making the work cumbersome. Furthermore, with conventional collection kits, if the operator is unfamiliar with handling the kit, there is a risk of a large amount of air entering the anaerobic culture bottle through the air vent.

[0009] The present invention aims to solve the problems described above. [Means for solving the problem]

[0010] (1) A first aspect of the present invention is a sampling kit comprising: an inlet tube to which a medical bag containing a sample to be collected is connected; a storage section connected downstream of the inlet tube and having a first storage chamber and a second storage chamber capable of containing the sample to be collected; an air vent connected to the upper part of the first storage chamber for discharging air from the storage section; and at least one adapter connected to the first storage chamber or the second storage chamber and connectable to a culture bottle, wherein a connecting passage is formed at the upper part of the storage section to connect the first storage chamber and the second storage chamber, the adapter has a transfer tube for transferring the sample to be collected from the first storage chamber or the second storage chamber to the culture bottle, and a hydrophilic filter is disposed on the transfer tube.

[0011] This sampling kit minimizes the number of times tubes need to be joined and separated during the process of collecting samples into culture bottles, resulting in superior work efficiency. A hydrophilic filter, which becomes less permeable to air when wet, is placed in the adapter's transfer tube, reducing the risk of large amounts of air entering the culture bottle. Therefore, the culture bottle can be used for culture testing as a culture bottle for anaerobic bacteria.

[0012] (2) In the sampling kit described in item (1) above, the containment section may have a partition wall separating the first containment chamber and the second containment chamber, and the connecting passage may be formed above the partition wall.

[0013] This makes it possible to construct a housing unit with a simple configuration in which the first housing chamber and the second housing chamber are connected by a connecting passage.

[0014] (3) In the sampling kit described in item (1) or (2) above, the air vent may have a lid that can be opened and closed.

[0015] This configuration allows for a more effective reduction in the risk of a large amount of air entering the culture bottle when transferring the sample to the culture bottle, by closing the air vent lid after collecting the sample in the containment section.

[0016] (4) In the sampling kit described in any one of the above items (1) to (3), the at least one adapter may have a first adapter connected to the first containment chamber and a second adapter connected to the second containment chamber.

[0017] Because a first adapter and a second adapter are provided, the target material can be collected into two culture bottles simultaneously. This improves the efficiency of culture testing.

[0018] (5) In the sampling kit described in any one of the above items (1) to (3), the at least one adapter may be a single adapter connected to only one of the first containment chamber and the second containment chamber.

[0019] With this configuration, there is little to no air introduced into the second culture bottle connected to the adapter. Therefore, the second culture bottle is particularly suitable as a culture bottle for anaerobic bacteria.

[0020] (6) In the collection kit described in any one of the above items (1) to (5), the first containment chamber and the second containment chamber may be integrated to form the containment section.

[0021] (7) A second aspect of the present invention is a collection method for collecting a sample into two culture bottles using a collection kit, the collection kit comprising: an inlet tube to which a medical bag containing the sample is connected; a storage section connected downstream of the inlet tube and having a first storage chamber and a second storage chamber capable of containing the sample; an air vent connected to the upper part of the first storage chamber for discharging air from the storage section; and at least one adapter connected to the first storage chamber or the second storage chamber and capable of being connected to a culture bottle, wherein the upper part of the storage section comprises the first storage chamber and the second storage chamber A connecting passage is formed, the adapter has a transfer tube for transferring the sample to be collected from the first or second containment chamber to the culture bottle, a hydrophilic filter is placed on the transfer tube, and the sampling method comprises a joining step of joining the medical bag to the inflow tube, an introduction step of introducing the sample to be collected into the first and second containment chambers via the inflow tube, and a transfer step of connecting the two culture bottles to at least one adapter simultaneously or sequentially to transfer the sample to be collected from the containment section to the two culture bottles.

[0022] (8) In the sampling method according to item (7) above, the at least one adapter has a first adapter connected to the first storage chamber and a second adapter connected to the second storage chamber, the two culture bottles are a first culture bottle and a second culture bottle, and in the transfer step, the first culture bottle may be connected to the first adapter and the second culture bottle may be connected to the second adapter.

[0023] (9) In the sampling method according to item (7) above, the at least one adapter is a single adapter connected to only one of the first storage chamber and the second storage chamber, and the transfer step includes a first transfer step of connecting a first culture bottle to the adapter and transferring the sampling target from the one storage chamber to the first culture bottle, a step of removing the first culture bottle from the adapter, a movement step of moving the sampling target from the other storage chamber of the first storage chamber and the second storage chamber to the one storage chamber, and a second transfer step of connecting a second culture bottle to the adapter and transferring the sampling target to the second culture bottle.

Advantages of the Invention

[0024] According to the present invention, in the operation of sampling a sampling target into a culture bottle using a sampling kit, the number of times of joining and separating the tubes can be minimized, so that the working efficiency is excellent. Since a hydrophilic filter having a property of making it difficult for air to pass through when wet is disposed in the transfer tube of the adapter, the risk of a large amount of air being mixed into the culture bottle can be reduced. Therefore, the culture bottle can be used for a culture test as a culture bottle for anaerobic bacteria.

Brief Description of the Drawings

[0025] [Figure 1] FIG. 1 is an overall schematic view of a sampling kit according to the first embodiment. [Figure 2] FIG.  2 is a first diagram for explaining a method of using the sampling kit according to the first embodiment. [Figure 3]Figure 3 is a second diagram illustrating the method of using the collection kit according to the first embodiment. [Figure 4] Figure 4 is a third diagram illustrating the method of using the collection kit according to the first embodiment. [Figure 5] Figure 5 is an overall schematic diagram of the sampling kit according to the second embodiment. [Figure 6] Figure 6 is the first diagram illustrating the method of using the collection kit according to the second embodiment. [Figure 7] Figure 7 is a second diagram illustrating the method of using the collection kit according to the second embodiment. [Figure 8] Figure 8 is a third diagram illustrating the method of using the collection kit according to the second embodiment. [Figure 9] Figure 9 is a fourth diagram illustrating the method of using the collection kit according to the second embodiment. [Figure 10] Figure 10 is a fifth diagram illustrating the method of using the collection kit according to the second embodiment. [Modes for carrying out the invention]

[0026] The collection kit 10 according to the first embodiment shown in Figure 1 is used to transfer the blood product, which is the sample to be collected, to culture bottles B1 and B2. The collection kit 10 is used, for example, in a blood center or other facility that manufactures blood products, for culture tests to confirm the safety of the blood product. In the culture test, tests are performed for the culture of anaerobic bacteria and aerobic bacteria. Therefore, a total of two culture bottles are used in the culture test: one for aerobic culture and one for anaerobic culture. Of the two culture bottles B1 and B2, one culture bottle is used for testing as an aerobic bacteria culture bottle, and the other culture bottle is used for testing as an anaerobic bacteria culture bottle. Hereinafter, culture bottles B1 and B2 will also be referred to as "first culture bottle B1" and "second culture bottle B2," respectively.

[0027] In the following description of the first embodiment (and the second embodiment described later), an example in which a platelet preparation is used as the blood product will be explained. However, the blood product collected by the collection kits 10 and 10A may also be a red blood cell preparation, a plasma preparation, or a whole blood preparation.

[0028] The sampling kit 10 comprises an inlet tube 12, a housing section 14, an air vent 16, a first adapter 21, and a second adapter 22. In the sampling kit 10, based on the arrangement during use, the direction in which the inlet tube 12 and air vent 16 are located is referred to as the upper side or upward, and the direction in which the first adapter 21 and second adapter 22 are located is referred to as the lower side or downward.

[0029] The inlet tube 12 is a translucent medical tube made of a thermoplastic resin such as polyvinyl chloride resin. The inlet tube 12 can be connected to or separated from other medical tubes without exposing its interior to the outside air, for example, by an aseptic bonding device or a tube sealer. The inlet tube 12 has an upstream first end 121 and a downstream second end 122. The first end 121 is initially welded and sealed. The second end 122 is connected to the upper end of the housing section 14.

[0030] The containment section 14 is connected to the downstream end (second end 122) of the inflow tube 12. The containment section 14 is made of a rigid material. Examples of materials used to make up the containment section 14 include rigid resin materials such as polypropylene, polyethylene, cyclic polyolefin, and polyacetal resin. The containment section 14 has a first containment chamber 31 and a second containment chamber 32 capable of containing the sample to be collected. The first containment chamber 31 and the second containment chamber 32 together form the containment section 14. The containment section 14 has a capacity of, for example, about 20 mL. The first containment chamber 31 has a volume that can contain a predetermined amount (for example, about 10 mL) of platelet preparation. The second containment chamber 32 has a volume that can contain a predetermined amount (for example, about 10 mL) of platelet preparation. The capacities of the first containment chamber 31 and the second containment chamber 32 are the same.

[0031] The storage section 14 has a partition wall 26 that separates the first storage chamber 31 and the second storage chamber 32. The partition wall 26 extends upward from the lower part (bottom) of the storage section 14. A connecting passage 33 is formed in the upper part of the storage section 14, connecting the first storage chamber 31 and the second storage chamber 32. The connecting passage 33 is formed at the upper end of the partition wall 26. An inlet tube 12 is connected to the storage section 14 at a position directly above the connecting passage 33.

[0032] The inflow tube 12 may be connected to the housing section 14 at a point offset from the communication passage 33 in the left-right direction of the housing section 14 in Figure 1. That is, the inflow tube 12 may be connected to the first housing chamber 31, or to the second housing chamber 32.

[0033] The air vent 16 is connected to the top of the housing section 14 and discharges air from the housing section 14. The air vent 16 has a vent pipe 161 that constitutes the vent body and a lid 162 that can open and close the opening of the vent pipe 161. A sterile filter 163 is placed inside the vent pipe 161. The lid 162 can airtightly close the opening of the vent pipe 161. When the lid 162 is opened, the inside of the housing section 14 (first housing chamber 31 and second housing chamber 32) is opened to the atmosphere. When the lid 162 is closed, the inflow of air from the outside to the inside of the housing section 14 is prevented. In Figure 1, the air vent 16 is connected to the top of the first housing chamber 31, but the air vent 16 may also be connected to the top of the second housing chamber 32.

[0034] The first adapter 21 is connected to the lower part of the housing section 14. The first adapter 21 communicates with the first housing chamber 31 and can be connected to the first culture bottle B1. The first adapter 21 comprises a housing cylinder 34 capable of housing the top of the first culture bottle B1, a transfer tube 38 having a tubular needle portion 36 positioned inside the housing cylinder 34, and a rubber cover 40 covering the needle portion 36. The housing cylinder 34 has an openable and closable lid 341. However, the lid 341 is not required. If the lid 341 is not provided, the opening of the housing cylinder 34 may be sealed with a sealant such as a thin film. The housing cylinder 34 can be opened by peeling off the sealant when in use. The needle portion 36 can puncture the rubber stopper attached to the top of the first culture bottle B1. When the needle portion 36 of the first adapter 21 penetrates the rubber stopper of the first culture bottle B1, the first culture bottle B1 and the first housing chamber 31 communicate. When the needle portion 36 punctures the rubber stopper of the first culture bottle B1, the rubber cover 40 is pushed inward towards the proximal end, causing the needle portion 36 to penetrate the rubber cover 40. When the needle portion 36 is withdrawn from the rubber stopper of the first culture bottle B1, the rubber cover 40 stretches due to its elastic restorative force and covers the needle portion 36 again.

[0035] The second adapter 22 is connected to the lower part of the housing section 14. The second adapter 22 communicates with the second housing chamber 32 and can be connected to the second culture bottle B2. The second adapter 22 has the same configuration as the first adapter 21. When the needle portion 36 of the second adapter 22 penetrates the rubber stopper of the second culture bottle B2, the second culture bottle B2 and the second housing chamber 32 communicate with each other.

[0036] A hydrophilic filter 24 is located in the transfer pipe 38 of the first adapter 21. A hydrophilic filter 24 is located in the transfer pipe 38 of the second adapter 22. Specifically, a hydrophilic filter 24 is fixed to the upper end (upstream end) of each transfer pipe 38. The hydrophilic filters 24 are located at the bottom of the first containment chamber 31 and the bottom of the second containment chamber 32, respectively. The hydrophilic filter 24 has the property of making it difficult for gas to pass through when wet. Hereinafter, the hydrophilic filter 24 located in the transfer pipe 38 of the first adapter 21 may be referred to as "hydrophilic filter 24a". The hydrophilic filter 24 located in the transfer pipe 38 of the second adapter 22 may be referred to as "hydrophilic filter 24b".

[0037] The collection kit 10 configured as described above is used as follows. In this embodiment, a collection method is described in which platelet preparations are dispensed into two culture bottles B1 and B2 using the collection kit 10.

[0038] As shown in Figure 2, the platelet bag 50 is attached to the collection kit 10. The platelet bag 50 is a medical bag containing a platelet preparation. The platelet bag 50 has a connecting tube 52. The tube 52 is attached to the inflow tube 12. The tube 52 is attached to the inflow tube 12 using a sterile joining device without coming into contact with the outside air.

[0039] Next, as shown in Figure 3, the platelet preparation is introduced into the containment section 14 (introduction step). In the introduction step, the platelet bag 50 is positioned above the containment section 14, and the lid 162 of the air vent 16 is opened. The platelet preparation flows out of the platelet bag 50 by gravity and is stored in the containment section 14 via the tube 52 and the inflow tube 12.

[0040] The platelet preparation is stored in the first storage chamber 31 and the second storage chamber 32. In this case, the platelet preparation is stored from the bottom of the first storage chamber 31 and the second storage chamber 32, and the liquid level of the platelet preparation rises in the first storage chamber 31 and the second storage chamber 32, respectively. As the liquid level of the platelet preparation rises in the first storage chamber 31 and the second storage chamber 32, the air inside the first storage chamber 31 and the second storage chamber 32 is pushed out by the platelet preparation and discharged to the outside of the storage section 14 through the air vent 16.

[0041] Eventually, the first containment chamber 31 and the second containment chamber 32 are filled with platelet preparations. This completes the introduction of the platelet preparations into the containment section 14. Each of the first containment chamber 31 and the second containment chamber 32 contains a predetermined amount (for example, about 10 mL) of platelet preparation. After the introduction process is complete, the lid 162 of the air vent 16 is closed.

[0042] Subsequently, the platelet bag 50 is separated from the inflow tube 12 using a tube sealer (high-frequency sealer or ultrasonic sealer). The tube sealer separates the inflow tube 12 from the tube 52 of the platelet bag 50 and simultaneously seals the first end 121 of the inflow tube 12 by welding. The separation of the inflow tube 12 and tube 52 is performed without exposing the internal flow path to the outside air.

[0043] The removed platelet bag 50 is stored until the culture test is complete before being made available for use. Meanwhile, the collection kit 10 filled with platelet preparation is brought into the clean bench. Next, the user performs the operation to transfer the platelet preparation from the collection kit 10 to the first culture bottle B1 and the second culture bottle B2.

[0044] As shown in Figure 4, the sample to be collected is transferred from the containment section 14 to two culture bottles B1 and B2 (transfer process). In the transfer process, the first culture bottle B1 is connected to the first adapter 21, and the second culture bottle B2 is connected to the second adapter 22. As the first culture bottle B1 is connected to the first adapter 21, the needle portion 36 of the first adapter 21 penetrates the rubber stopper of the first culture bottle B1. The platelet preparation in the first containment chamber 31 is drawn out by the negative pressure of the first culture bottle B1, and a predetermined amount (for example, about 10 mL) of platelet preparation is introduced into the first culture bottle B1 via the hydrophilic filter 24a and the transfer tube 38. Similarly, as the second culture bottle B2 is connected to the second adapter 22, a predetermined amount (for example, about 10 mL) of platelet preparation is introduced into the second culture bottle B2 via the hydrophilic filter 24b and the transfer tube 38. Next, the first adapter 21 is removed from the first culture bottle B1, and the second adapter 22 is removed from the second culture bottle B2.

[0045] When platelet preparations are collected into the first culture bottle B1 and the second culture bottle B2, the two hydrophilic filters 24a and 24b are wet with the platelet preparations. The hydrophilic filters 24 have the property of making it difficult for gas to pass through when wet. Therefore, when all of the platelet preparation is discharged from the first containment chamber 31 (when the outer surface of the hydrophilic filter 24a is exposed to the space inside the first containment chamber 31), suction by the first culture bottle B1 stops. Similarly, when all of the platelet preparation is discharged from the second containment chamber 32 (when the outer surface of the hydrophilic filter 24b is exposed to the space inside the second containment chamber 32), suction by the first culture bottle B1 stops. The only air that flows into the first culture bottle B1 is the air that was present in the flow path within the transfer tube 38 (including the needle portion 36) of the first adapter 21. Therefore, there is little air contamination in the first culture bottle B1. The air flowing into the second culture bottle B2 is only the air that was present in the flow path within the transfer tube 38 (including the needle portion 36) of the second adapter 22. Therefore, there is minimal air contamination of the second culture bottle B2.

[0046] When collecting platelet preparations into culture bottles B1 and B2, the lid 162 of the air vent 16 may be open, but it is preferable that it be closed, as shown in Figure 4. By closing the lid 162 of the air vent 16 when collecting platelet preparations into culture bottles B1 and B2, the inflow of air into the containment section 14 via the air vent 16 is prevented. This effectively reduces the amount of air mixed into culture bottles B1 and B2.

[0047] Once a predetermined amount of platelet preparation has been introduced into the first culture bottle B1, the first adapter 21 is removed from the first culture bottle B1. Once a predetermined amount of platelet preparation has been introduced into the second culture bottle B2, the second adapter 22 is removed from the second culture bottle B2.

[0048] The above steps complete the collection of platelet preparations using the collection kit 10. One of the first culture bottle B1 and the second culture bottle B2 is used for testing as an aerobic bacteria culture bottle, and the other is used for testing as an anaerobic bacteria culture bottle. Since both the first culture bottle B1 and the second culture bottle B2 contain little air, either one can be used as the anaerobic bacteria culture bottle.

[0049] Note that the connection of the first adapter 21 to the first culture bottle B1 and the connection of the second adapter 22 to the second culture bottle B2 may be performed at different times. In this case as well, since the hydrophilic filter 24 is wet, there will be little air entering the first culture bottle B1 and the second culture bottle B2.

[0050] The first embodiment provides the following effects.

[0051] In the process of collecting samples into culture bottles B1 and B2 using the collection kit 10, the number of times tubes are joined and separated is minimized, resulting in superior work efficiency. Since the hydrophilic filter 24, which has the property of making it difficult for air to pass through when wet, is placed in the transfer tube 38 of the first adapter 21 and the second adapter 22, the risk of a large amount of air entering the culture bottle can be reduced. For this reason, the culture bottle can be used for culture testing as a culture bottle for anaerobic bacteria. By connecting the first adapter 21 to the first culture bottle B1 and the second adapter 22 to the second culture bottle B2 simultaneously, samples can be collected into both culture bottles B1 and B2 at the same time, which is efficient.

[0052] Furthermore, since it eliminates the need to measure and collect the sample by eye according to the scale lines, the work becomes easier and measurement errors can be reduced. Therefore, the sampling kit 10 can suppress variations in the amount of sample to be introduced into the first culture bottle B1 and the second culture bottle B2.

[0053] The storage section 14 has a partition wall 26 that separates the first storage chamber 31 and the second storage chamber 32, and a connecting passage 33 is formed above the partition wall 26. This allows for the construction of a storage section 14 with a simple structure in which the first storage chamber 31 and the second storage chamber 32 are connected via the connecting passage 33.

[0054] The air vent 16 has an openable and closable lid 162. With this configuration, after collecting the sample to be collected into the containment section 14, closing the lid 162 of the air vent 16 makes it possible to more effectively reduce the risk of a large amount of air entering the culture bottles B1 and B2 when transferring the sample to the culture bottles B1 and B2.

[0055] The sample collection kit 10 includes a first adapter 21 connected to a first containment chamber 31 and a second adapter 22 connected to a second containment chamber 32. Because both the first adapter 21 and the second adapter 22 are provided, samples can be collected simultaneously into two culture bottles B1 and B2. This improves the efficiency of the culture testing process.

[0056] The sampling kit 10A according to the second embodiment shown in Figure 5 differs from the sampling kit 10 shown in Figures 1 to 4, which has a first adapter 21 and a second adapter 22, in that it has a single adapter 20. In Figure 5, the adapter 20 is connected to the second containment chamber 32. Alternatively, the adapter 20 may be connected to the first containment chamber 31 instead of the second containment chamber 32. The configuration of the adapter 20 is the same as that of the first adapter 21 (or second adapter 22) shown in Figure 1, etc.

[0057] The collection kit 10A, configured as described above, is used as follows. In the second embodiment, a collection method in which platelet preparations are dispensed into two culture bottles B1 and B2 using the collection kit 10A will be described. In the method of using the collection kit 10A, the same parts as the method of using the collection kit 10 shown in Figure 1 will be omitted from the explanation.

[0058] As shown in Figure 6, the platelet bag 50 is aseptically attached to the collection kit 10A (attachment step). Next, as shown in Figure 7, the lid 162 of the air vent 16 is opened, and the platelet preparation flows out of the platelet bag 50 and is stored in the storage section 14 via the tube 52 and the inflow tube 12 (introduction step). A predetermined amount (for example, about 10 mL) of platelet preparation is placed in the first storage chamber 31 and the second storage chamber 32, respectively. After the introduction step is completed, the lid 162 of the air vent 16 is closed.

[0059] Subsequently, the platelet bag 50 is separated from the inflow tube 12 using a tube sealer (high-frequency sealer or ultrasonic sealer). The removed platelet bag 50 is stored until the culture test is completed before being put into use. Meanwhile, the collection kit 10A filled with platelet preparation is brought into a clean bench. Next, the user performs the operation to transfer the platelet preparation from the collection kit 10A to the first culture bottle B1 and the second culture bottle B2 (transfer process).

[0060] As shown in Figure 8, in the transfer process, the sample to be collected is first transferred to the first culture bottle B1 (first transfer process). In the first transfer process, it is preferable that the lid 162 of the air vent 16 remains closed. In the first transfer process, the first culture bottle B1 is connected to the adapter 20. The platelet preparation in the first containment chamber 31 is drawn out by the negative pressure of the culture bottle B1, and a predetermined amount (for example, about 10 mL) of platelet preparation is introduced into the culture bottle B1 via the hydrophilic filter 24 and the transfer tube 38. Once the predetermined amount of platelet preparation has been introduced into the culture bottle B1, the adapter 20 is removed from the culture bottle B1. Since the first containment chamber 31 and the second containment chamber 32 are separated by a partition wall 26, platelet preparation remains in the first containment chamber 31.

[0061] Next, the sample to be collected is moved from the first storage chamber 31 to the second storage chamber 32 (movement process). Specifically, the collection kit 10A is turned on its side, and the platelet preparation is moved from the first storage chamber 31 to the second storage chamber 32 via the connecting passage 33. As a result, as shown in Figure 9, the collection kit 10A is in a state where the platelet preparation is contained in the second storage chamber 32, and the platelet preparation is not contained in the first storage chamber 31.

[0062] Next, as shown in Figure 10, the sample to be collected is transferred to the second culture bottle B2 (second transfer step). In the second transfer step, it is preferable that the lid 162 of the air vent 16 remains closed. In the second transfer step, culture bottle B2 is connected to the adapter 20. With this connection, a predetermined amount (for example, about 10 mL) of platelet preparation is introduced into culture bottle B2. Once the predetermined amount of platelet preparation has been introduced into culture bottle B2, the adapter 20 is removed from culture bottle B2.

[0063] Through the above steps, the collection of platelet preparations using the collection kit 10A is completed. One of the culture bottles B1 and B2 is used for testing as an aerobic bacteria culture bottle, and the other is used for testing as an anaerobic bacteria culture bottle. The air that was initially present in the flow path of the transfer tube 38 is recovered into the first culture bottle B1 when the platelet preparation is collected in the first culture bottle B1, so there is little to no air contamination into the second culture bottle B2. Therefore, the second culture bottle B2 is particularly suitable as an anaerobic bacteria culture bottle.

[0064] Furthermore, the present invention is not limited to the disclosure described above, and can take various configurations without departing from the spirit of the invention. [Explanation of symbols]

[0065] 10, 10A... Sampling kit 12... Inlet tube 14…Containment area 16…Air vent 21...First adapter 22...Second adapter 24, 24a, 24b... Hydrophilic filters 33... Connecting passages

Claims

1. An inlet tube to which a medical bag containing the sample to be collected is connected, A containment unit connected downstream of the inflow tube, having a first containment chamber and a second containment chamber capable of containing the object to be collected, An air vent connected to the upper part of the first containment chamber, which discharges air from the containment section, The system comprises at least one adapter connected to the first or second containment chamber and connectable to a culture bottle, A connecting passage is formed in the upper part of the aforementioned storage section, which connects the first storage chamber and the second storage chamber. The adapter has a transfer tube for transferring the sample to be collected from the first or second storage chamber to the culture bottle, and a hydrophilic filter is placed in the transfer tube, in the sampling kit.

2. In the sampling kit according to claim 1, The storage section has a partition wall separating the first storage chamber and the second storage chamber, and the communication passage is formed above the partition wall in the sampling kit.

3. In the sampling kit according to claim 1 or 2, The aforementioned air vent is a collection kit having a lid that can be opened and closed.

4. In the sampling kit according to claim 1 or 2, The sampling kit comprises at least one adapter, a first adapter connected to the first containment chamber, and a second adapter connected to the second containment chamber.

5. In the sampling kit according to claim 1 or 2, The sampling kit wherein the at least one adapter is a single adapter connected to only one of the first containment chamber and the second containment chamber.

6. In the sampling kit according to claim 1 or 2, A sampling kit in which the first containment chamber and the second containment chamber together form the containment section.

7. A sampling method in which the sample to be collected is collected into two culture bottles using a sampling kit, The aforementioned collection kit is An inlet tube to which a medical bag containing the sample to be collected is connected, A containment unit connected downstream of the inflow tube, having a first containment chamber and a second containment chamber capable of containing the object to be collected, An air vent connected to the upper part of the first containment chamber, which discharges air from the containment section, The system comprises at least one adapter connected to the first or second containment chamber and connectable to a culture bottle, A connecting passage is formed in the upper part of the aforementioned storage section, which connects the first storage chamber and the second storage chamber. The adapter has a transfer tube for transferring the sample to be collected from the first or second containment chamber to the culture bottle, and a hydrophilic filter is placed in the transfer tube. The aforementioned sampling method is, A joining step of joining the medical bag to the inlet tube, An introduction step of introducing the sample to be collected into the first and second containment chambers via the inflow tube, A sampling method comprising a transfer step of connecting the two culture bottles to at least one adapter simultaneously or sequentially, thereby transferring the sample to be collected from the storage unit to the two culture bottles.

8. In the sampling method according to claim 7, The at least one adapter comprises a first adapter connected to the first containment chamber and a second adapter connected to the second containment chamber. The two culture bottles mentioned above are the first culture bottle and the second culture bottle. The sampling method involves connecting the first culture bottle to the first adapter and the second culture bottle to the second adapter during the transfer process.

9. In the sampling method according to claim 7, The at least one adapter is a single adapter connected to only one of the first and second accommodation chambers, The aforementioned transfer process is, A first transfer step involves connecting the first culture bottle to the adapter and transferring the sample to be collected from one of the storage chambers to the first culture bottle. The steps include removing the first culture bottle from the adapter, A transfer step of moving the object to be collected from the other of the first and second containment chambers to the first containment chamber, A sampling method comprising a second transfer step of connecting a second culture bottle to the adapter and transferring the sample to be collected into the second culture bottle.

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