Antimicrobial and antiviral agent, antimicrobial and antiviral component, and method for producing an antimicrobial and antiviral agent

Abstract

It uses naturally derived active ingredients to combat various types of bacteria, including fungi, and viruses. and to provide an effective antibacterial and antiviral agent. [Solution] Antibacterial and antiviral agent with Lactobacillus from mugwort S medicine.

Description

Technical Field

[0001] The present invention relates to an antibacterial and antiviral drug, an antibacterial and antiviral member, and a method for producing an antibacterial and antiviral drug. It relates to.

Background Art

[0002] Some lactic acid bacteria are known to counter pathogenic bacteria harmful to humans and animals. For example, Patent Document 1 describes using the pentosus species of the genus Lactobacillus, which is a bacterium derived from Alpinia speciosa, to sterilize Pyrococcus bacteria. However, Patent Document 1 states that the concentrated fermentation broth of Alpinia speciosa has no effect on Candida bacteria.

[0003] Patent Document 2 states that the reuteri species of the genus Lactobacillus produces an antimicrobial substance called reuterin and inhibits the growth of bacteria such as Salmonella and some fungi. Also, Patent Document 2 describes using the fermentum species of the genus Lactobacillus for the treatment and prevention of skin disorders. Furthermore, Patent Document 2 also describes using the reuteri species of the genus Lactobacillus for the prevention or treatment of skin disorders caused by pathogenic bacteria such as Staphylococcus aureus, Streptococcus pyogenes, and Propionibacterium acnes, and for the prevention or treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections. [[ID=SS]] (MRSA) infections. <P

[0004] Patent Document 3 states that the plantarum species of the genus Lactobacillus suppresses the growth of Gram-negative bacteria and Gram-positive bacteria such as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus, but does not suppress the growth of fungi such as Candida albicans and Candida parapsilosis. It describes that. <P

[0005] Patent document 4 describes how Lactobacillus casei strain KE01 reduces E. coli over several weeks. It is stated that the Lactobacillus gasserii species is a salmon It describes reducing Nera enteritidis. Patent document 6 describes yeast and A biofilm consisting of Ctobacillus plantarum ML11-11 strain reduces Bacillus subtilis. It describes what to do.

[0006] Patent document 7 describes how the Lactobacillus pentosus species inhibits the proliferation of Candida. It states that... However, Patent Document 7 states that the Lactobacillus pentosus species is It does not mention reducing Candida. [Prior art documents] [Patent Documents]

[0007] [Patent Document 1] Japanese Patent Publication No. 2015-193619 [Patent Document 2] Japanese Patent Publication No. 2014-230541 [Patent Document 3] Japanese Patent Publication No. 2010-215641 [Patent Document 4] Japanese Patent Publication No. 2010-195778 [Patent Document 5] Japanese Patent Publication No. 2012-147759 [Patent Document 6] Japanese Patent Publication No. 2013-150598 [Patent Document 7] Japanese Patent Publication No. 2007-308504 [Overview of the project] [Problems that the invention aims to solve]

[0008] Alcohol is sometimes used as an antibacterial agent, but Bacillus cereus, Bacillus subtilis, and Bacillus natto are also present. Bacteria that form spores, such as , have alcohol resistance. In addition, alcohol may cause side effects such as rashes, and skin irritation symptoms. Furthermore, antibacterial drugs containing synthetic compounds as active ingredients may also stimulate the affected area or cause swelling symptoms accompanied by itching and pain. Therefore, there is a need for antibacterial and antiviral drugs that are effective against various types of fungi and viruses and contain natural-derived ingredients as active ingredients. The present invention has been made in view of such circumstances, and provides an antibacterial and antiviral drug, an antibacterial and antiviral member, and a method for producing an antibacterial and antiviral drug that are effective against various types of fungi and viruses and contain natural-derived ingredients as active ingredients. bacteria and viruses, and aims to provide a method for producing an antibacterial and antiviral drug.

Means for Solving the Problems

[0009] The antibacterial and antiviral drug according to one aspect of the present invention includes Lactobacillus belonging to the genus Lactobacillus derived from mugwort. In addition, the antibacterial and antiviral drug according to one aspect of the present invention includes secretions of Lactobacillus belonging to the genus Lactobacillus derived from mugwort. Further, the antibacterial and antiviral drug according to one aspect of the present invention includes Lactobacillus belonging to the genus Lactobacillus, and the species of Lactobacillus is at least one selected from the group consisting of parafarragin is species, parabuchneri species, buchneri species, harbinensis species , vini species, and nagelii species. In addition, the antibacterial and antiviral drug according to one aspect of the present invention includes secretions of Lactobacillus, and the species of Lactobacillus is at least one selected from the group consisting of parafarraginis species, parabuchneri species , buchneri species, harbinensis species, vini species, and nagelii species . Furthermore, the antibacterial and antiviral drug according to one aspect of the present invention The virus member includes a member and the above-mentioned antibacterial and antiviral drug disposed on the surface of the member. Furthermore, the method for producing an antibacterial and antiviral drug according to one aspect of the present invention includes fermenting a plant to obtain a fermentation broth containing Lactobacillus.

Advantages of the Invention

[0010] According to the present invention, it is possible to provide an antibacterial and antiviral drug, an antibacterial and antiviral member, and a method for producing an antibacterial and antiviral drug that are effective against bacteria and viruses including various types of fungi, with natural-derived components as active ingredients.

Brief Description of the Drawings

[0011] [Figure 1] It is a graph and a table showing the analysis results of the bacteria contained in the fermentation broth according to Example 1. [Figure 2] It is a graph and a table showing the analysis results of the bacteria contained in the fermentation broth according to Example 2. [Figure 3] It is a graph and a table showing the antibacterial effect of the fermentation broth according to Example 4. [Figure 4] It is a graph and a table showing the antifungal effect of the fermentation broth according to Example 5. [Figure 5] It is a graph and a table showing the antiviral effect of the fermentation broth according to Example 6. [Figure 6] It is a graph and a table showing the antiviral effect of the fermentation broth according to Example 6. [Figure 7] It is a graph and a table showing the effect of the fermentation broth according to Example 7 against Trichophyton rubrum.

Modes for Carrying Out the Invention

[0012] Hereinafter, embodiments of the present invention will be described in detail. The following-described embodiments illustrate devices and methods for embodying the technical idea of this invention, and are not intended to limit the scope of this invention The technical concept does not limit the combination of constituent elements, etc., to the following. The technical concept may be modified in various ways within the scope of the patent claims.

[0013] (First Embodiment) The antibacterial and antiviral agent according to the first embodiment of the present invention is Lactobacillus (Lactobacillus). At least one selected from the group consisting of secretions from the genera *Acillus* and *Lactobacillus*. It includes one. Lactobacillus is a type of lactic acid bacillus, a gram-positive, facultative anaerobic bacterium. Yes. Lactobacillus species produce lactic acid by fermenting sugars. Lactobacillus species are known to infect humans. Although they also inhabit the bodies of animals, the Lactobacillus genus in the first embodiment is Plant-derived Lactobacillus species are preferred.

[0014] For example, the Lactobacillus genus according to the first embodiment is extracted by fermenting plants. Examples of plants include mugwort, angelica tree, longevity grass, and cocoa, but are not limited to these. No. Lactobacillus species derived from mugwort, for example, the species parafarraginis. , species parabuchneri, species buchneri, and species harbinensis, etc. This includes Lactobacillus species derived from Angelica keiskei, such as Lactobacillus vini and Lactobacillus nagelii. The antimicrobial and antiviral agent according to the first embodiment includes multiple species of the genus Lactobacillus. You can stay like that.

[0015] The antibacterial and antiviral agent according to the first embodiment reduces fungi (mold). Antimicrobial and antiviral drugs in this form, for example, can kill fungi by more than 80% within 24 hours, or 85%. Reduce by more than %, more than 90%, or more than 95%. Examples of fungi include dermatophytes and Candida. Examples include, but are not limited to, Cryptococcus and Aspergillus. The antibacterial and antiviral agent according to Embodiment 1 can be used as a treatment for fungal infections. Examples of these diseases include tinea, candidiasis, cryptococcosis, and aspergillosis. However, it is not limited to these.

[0016] The antibacterial and antiviral agent according to the first embodiment reduces Gram-negative and Gram-positive bacteria. The antibacterial and antiviral agent according to the first embodiment is, for example, effective against Gram-negative bacteria and Gram-positive bacteria. Reduce the bacterial population by 80%, 85%, 90%, or 95% or more within 24 hours. To reduce. Gram-negative bacteria include E. coli, Salmonella, Vibrio parahaemolyticus, Klebsiella pneumoniae, Examples include, but are not limited to, Pseudomonas aeruginosa. Gram-positive bacteria include Methicillin. Examples include iodine-resistant Staphylococcus aureus (MRSA), spore-forming Bacillus cereus, and Bacillus subtilis. However, it is not limited to these. The antimicrobial and antiviral agent according to the first embodiment is Gram-negative. It can be used as a disinfectant for sexually transmitted bacteria and Gram-positive bacteria.

[0017] The antimicrobial and antiviral agent according to the first embodiment reduces the virus. Antimicrobial and antiviral drugs that address the condition, for example, can eliminate more than 80% of the virus within 24 hours, or 85%. Reduce by more than %, more than 90%, or more than 95%. Viruses have an envelope. Enveloped viruses, which are viruses that have an envelope, and non-enveloped viruses, which are viruses that do not have an envelope. Includes enveloped viruses. Furthermore, the viruses include DNA viruses and RNA viruses. Includes.

[0018] Examples of enveloped DNA viruses include human herpesviruses and vaccinia. Examples include, but are not limited to, viruses and hepatitis B virus.

[0019] Examples of enveloped RNA viruses include influenza virus and SARS-CoV-2. Ronavirus, RSV, mumps virus, lassa virus, dengue virus, cold Measles virus, human immunodeficiency virus, measles virus, hepatitis C virus, Ebola virus Examples include, but are not limited to, yellow fever virus and Japanese encephalitis virus.

[0020] Examples of DNA viruses that do not have an envelope include adenoviruses and B19 virus. Examples include, but are not limited to, papovavirus and human papillomavirus. .

[0021] RNA viruses that do not have an envelope include norovirus, poliovirus, and erythrocytes. Covirus, Hepatitis A virus, Hepatitis E virus, Rhinovirus, Astrovirus Examples include rotavirus, coxsackievirus, enterovirus, and sapovirus. These are some examples, but they are not limited to these.

[0022] The antimicrobial and antiviral agent according to the first embodiment can be used as a disinfectant for viruses. .

[0023] The antimicrobial and antiviral agent according to the first embodiment contains an effective amount of Lactobacillus species and / or Contains an effective amount of Lactobacillus secretions. An effective amount is defined as the amount that produces an antibacterial or antiviral effect. This is the amount necessary for treatment, and it is determined appropriately depending on the target bacteria, virus, and symptoms. The antibacterial and antiviral agent according to the first embodiment is, for example, 0.001% by weight or more, and 0.005% by weight. Contains Lactobacillus genus at a concentration of % by weight or 0.01% by weight or higher. Also, 1 The antibacterial and antiviral agents according to the embodiment may be, for example, 20% by weight or less, 15% by weight or less, It contains Lactobacillus at concentrations of 10% by weight or less, but Lactobacillus at higher concentrations It may contain [unclear]. However, a higher concentration of Lactobacillus tends to increase viscosity. .

[0024] The Lactobacillus species contained in the antibacterial and antiviral drug according to the first embodiment was a live bacterium. It may be, for example, heat-treated dead bacteria. Therefore, the first embodiment The antibacterial and antiviral agents related to this may contain dead bacteria of the Lactobacillus genus. For the genus Lactobacillus, dried cell material is also acceptable. Dead bacteria or dried cell material of the genus Lactobacillus are also acceptable. It exhibits antibacterial and antiviral effects. In addition, dead bacteria or dried bacterial cells of the Lactobacillus genus are It is easy to transport and store for long periods of time.

[0025] The antimicrobial and antiviral agent according to the first embodiment may be, for example, a liquid, cream, ointment, or plaster. It may be a gel, wax, or spray. Also, the antibacterial agent according to the first embodiment Antiviral drugs are used, for example, as topical skin medications such as disinfectants and ointments, eye drops, and oral medications. The antimicrobial and antiviral agent according to the first embodiment is, for example, used on the skin of the human body, including the fingers and toes. It is applicable to the skin, hair, oral cavity, and eyeballs, etc. Furthermore, it is an antibacterial and antiviral agent according to the first embodiment. The drug can also be applied to, for example, cooking utensils, the walls and floors of buildings such as hospitals, and furniture such as desks.

[0026] The antibacterial and antiviral agent according to the first embodiment includes, in addition to Lactobacillus species, liquid oils and solids. Body oils, waxes, hydrocarbons, higher fatty acids, higher alcohols, esters, silicones, anio Neopropyl surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, humectants, Water-soluble polymers, thickeners, coating agents, metal ion chelating agents, lower alcohols, polyhydric alcohols, Sugars, amino acids, organic amines, pH adjusters, skin nutrients, vitamins, antioxidants, It contains, as appropriate, ingredients for cosmetics and pharmaceuticals, such as fragrances, powders, colorants, and water, depending on the purpose. That's fine.

[0027] The antibacterial and antiviral agent according to the first embodiment contains antibacterial substances in addition to Lactobacillus species. The product may contain antiviral substances as appropriate, depending on the purpose.

[0028] The antibacterial and antiviral agent according to the first embodiment contains Lactobacillus species and its active ingredient. The secretions contain various types of fungi, bacteria, and viruses that can cause various diseases such as food poisoning. It is possible to reduce [the amount of [the substance]. Furthermore, the antibacterial and antiviral agent according to the first embodiment is effective. The components include Lactobacillus species and their secretions, which form spores that are resistant to alcohol. It is also possible to reduce Bacillus reus.

[0029] Furthermore, the antibacterial and antiviral drug according to the first embodiment contains Lactobacillus genus as an active ingredient. Because it is a naturally derived lactic acid bacterium, it is unlikely to have any adverse effects such as side effects when applied to humans. Furthermore, it is safe. Even if an antibacterial or antiviral drug is accidentally ingested by an infant, child, or elderly person, lactobacillus Shirasu are edible lactic acid bacteria and are easily broken down by digestive enzymes in the intestinal tract, thus avoiding side effects. These adverse effects are few or no. The same applies to secretions.

[0030] The antimicrobial and antiviral agent according to the first embodiment is obtained by fermenting plants and contains Lactobacillus species. It is produced by obtaining a fermented liquid. When fermenting plants, salt and sugars such as molasses are used. It is added to plants. The fermentation temperature is, for example, 30°C. The hydrogen ion concentration of the resulting fermentation liquid. The pH is around 4.0. Secretions from Lactobacillus species can be extracted from the fermentation liquid. stomach.

[0031] The resulting fermentation liquid may be heated to kill the Lactobacillus species present in it. Alternatively, the fermentation liquid may be spray-dried to obtain dried Lactobacillus cells. The dried cells are It can also be produced by freeze-drying and hot-air drying methods.

[0032] Furthermore, the obtained fermentation liquid, Lactobacillus cells, or Lactobacillus cells Dried ingredients may be added to soy milk, and the soy milk may be fermented to obtain fermented soy milk liquid. Fermented soy milk liquid may also be antibacterial. It has antiviral effects.

[0033] (Second Embodiment) In the second and subsequent embodiments, descriptions of matters common to the first embodiment will be omitted. Only the points will be explained. In particular, similar effects and benefits from similar configurations will be explained for each embodiment. I will not mention them individually.

[0034] The antibacterial and antiviral member according to the second embodiment comprises a member and a first member disposed on the surface of the member. The invention comprises an antibacterial and antiviral agent according to the embodiment, and a member which is a solid, for example, gold. It consists of materials such as wood, resin, glass, ceramics, and wood, but the materials are not limited to these. No. For example, when manufacturing the antibacterial and antiviral member according to the second embodiment, the first embodiment Antimicrobial and antiviral agents related to the form of application, paints, dyes, pigments, various resins, synthetic rubber, latex Materials containing antibacterial and antiviral agents, which are incorporated into materials such as films and fibers, are applied to the surface of the component. Apply, arrange, or laminate.

[0035] The concentration of antimicrobial and antiviral agents in the material is, for example, 0.001% by weight or more, 0.0 0.5% by weight or more, or 0.01% by weight or more, and 20% by weight or less, or 15% by weight or less. It may be, or less than 10% by weight, but is not limited to these. [Examples]

[0036] Embodiments of the present invention are described below. However, the present invention is not limited to the following embodiments. That is, of course.

[0037] (Example 1: Lactobacillus species derived from Artemisia princeps) For mugwort leaves, during the 2-hour period of the day, one hour before and one hour after sunrise. It is believed that the number of lactic acid bacteria is maximized during this time. Also, outside of this time period, the number of lactic acid bacteria decreases. It is believed that photosynthetic bacteria increase slightly. Therefore, during these two hours, the mugwort leaves Approximately 20 cm from the tip was collected. The 6.3 kg of mugwort leaves that were collected were immediately processed. Place it in the first pickling barrel lined with a plastic bag, and wrap it in mugwort leaves with 3.2 kg of molasses and 0. After sprinkling 6 kg of coarse salt, the opening of the plastic bag was closed and sealed. A weight was placed on top, and the mugwort leaves were pickled.

[0038] A few days after the pickling liquid had risen to the top of the mugwort leaves, the weight was removed. Next, into the second pickling barrel, Add 10 liters of chlorine-free water for rinsing, and put the pickled mugwort leaves into the water. I added 10 kg of pickling liquid. Furthermore, I prepared a third pickling barrel and placed a metal slab over the opening of the third pickling barrel. I placed a mesh filter on top. I took mugwort leaves little by little from the second pickling barrel, washing them by hand. Then, place the mugwort leaves on the wire mesh filter at the opening of the third pickling barrel, lightly pressing them down with your palm, and add the pickling liquid. I narrowed it down.

[0039] After squeezing all the mugwort leaves, pass the remaining pickling liquid from the second batch through a wire mesh filter. It was filtered. Next, molasses (Hateruma brown sugar) was added to the pickling liquid in the third pickling barrel until it reached a final concentration of 10 by weight. The coarse salt was dissolved in the mixture so that the final concentration was 3% by weight. Then, the third pickling process was carried out. Fermentation was initiated by raising the ambient temperature of the vat to approximately 30°C. Initially, large bubbles began to form. The foaming was observed, gradually changing to fine bubbles, and finally subsiding. Approximately one week later The pH was around 3.8 when the fizzing subsided. The pickling liquid at this time was used as a mugwort fermentation liquid. The obtained mugwort fermentation liquid was then heated at 70°C for 30 minutes to kill the bacteria. We obtained a fermented mugwort liquid.

[0040] Unheat-treated mugwort fermented liquid is used with next-generation sequencers (MiSeq, Illumina Analysis conducted by the company revealed, as shown in Figure 1, that the mugwort fermentation liquid contains Lactobacillus parenchyma. afarraginis species, parabuchneri species, buchneri species, and h It included species such as *arbinensis*. Furthermore, next-generation sequencers are high-throughput. Also called a sequencer. The numbers in the table in Figure 1 represent the bacterial species contained in the mugwort fermentation liquid. This reflects the number of bacteria.

[0041] (Example 2: Lactobacillus genus derived from Angelica keiskei) In the case of Ashitaba, the milk is released during the 2-hour period around sunrise, one hour before and one hour after. It is believed that the number of acid-producing bacteria is maximized during this time. Also, outside of this time period, the number of lactic acid bacteria decreases. It is believed that photosynthetic bacteria increase during this two-hour period. The stems were harvested. The 6.3 kg of harvested Angelica keiskei were immediately placed in a plastic bag. Place in pickling barrel 1, sprinkle 3.2 kg of molasses and 0.6 kg of coarse salt over the Angelica keiskei, then... I closed the opening of the plastic bag and sealed it. I placed a weight on top of the plastic bag and pickled the Angelica keiskei inside. .

[0042] A few days later, when the pickling liquid had risen to the top of the Angelica leaves, the weight was removed. Next, in the second pickling barrel, Add 10 liters of chlorine-free water for rinsing, and then add the pickled Angelica keiskei and 10 kg of other ingredients to the water. The pickling liquid was added. Furthermore, a third pickling barrel was prepared, and a wire mesh filter was placed over the opening of the third pickling barrel. I put the ta on. From the second pickling barrel, I took out the angelica little by little, rubbing and washing it by hand, and the third I lightly pressed the angelica leaves with my palms against the wire mesh filter on the opening of the pickling barrel and squeezed out the pickling liquid.

[0043] After squeezing all the Angelica keiskei, the remaining pickling liquid in the second batch was filtered through a wire mesh filter. Next, molasses was added to the pickling liquid in the third pickling barrel so that the final concentration was 10% by weight. Coarse salt was dissolved in the mixture to a final concentration of 3% by weight. Then, the ambient temperature of the third pickling barrel was adjusted to approximately Fermentation was initiated by raising the temperature to 30°C. Initially, large bubbles were observed, and gradually... The foaming gradually changed to fine bubbles, and finally subsided. About a week later, when the foaming had stopped. The pH was around 4.0. The pickling liquid at this time was used as the Angelica keiskei fermentation liquid. A portion of the Angelica keiskei fermentation liquid was heated at 70°C for 30 minutes to kill the bacteria, resulting in a heat-treated Angelica keiskei fermentation liquid. .

[0044] Unheat-treated Angelica keiskei fermented liquid is processed using a next-generation sequencer (MiSeq, Illumina). Analysis using the following method revealed, as shown in Figure 2, that the Angelica keiskei fermented liquid contains vini species and nagel species. It contained species ii, etc. The numbers in the table in Figure 2 represent the bacterial species contained in the Angelica keiskei fermentation liquid. This reflects the number of bacteria.

[0045] (Example 3: Soy milk fermented liquid using Lactobacillus genus) Soy milk was heated to 70°C and sterilized by heat treatment for approximately 30 minutes. To the heat-sterilized soy milk, the unheated mugwort fermented liquid prepared in Example 1 was added to a final concentration of approximately 10 wt%, and the mixture was thoroughly stirred. After that, heat treatment was performed. I haven't done it Soy milk to which mugwort fermentation liquid was added was fermented at 37°C for 24 hours. After fermentation, the solids were removed by filtration to obtain a soy milk ferment containing Lactobacillus species.

[0046] (Example 4: Antimicrobial effect of Lactobacillus species) We prepared Staphylococcus aureus and MRSA as Gram-positive cocci, and *Staphylococcus aureus* as a Gram-positive bacillus. We prepared herbaceous bacteria and Bacillus cereus, and Gram-negative cocci such as Escherichia coli, Salmonella, and Vibrio parahaemolyticus. We prepared Bacillus orientale and Klebsiella pneumoniae, and Pseudomonas aeruginosa as a Gram-negative bacillus.

[0047] In the 10 mL of soy milk ferment liquor containing Lactobacillus prepared in Example 1, the above bacteria were added. Choose 10 of these 7 A 0.1 mL bacterial solution containing a concentration of cells / mL was inoculated and allowed to react at 25°C over time. The number of viable bacteria in the inoculated sample was measured for 24 hours. A control sample with a concentration of 1 / 15 was also used. Inoculate 0.1 mL of bacterial suspension into 10 mL of mol / L, pH 7.2 phosphate buffer and store at 25°C. The bacteria were treated with the solution, and the number of viable bacteria in the inoculated bacteria was measured over 24 hours. As a result, as shown in Figure 3... Furthermore, the soy milk fermentation liquid containing Lactobacillus bacteria contains all types of bacteria prepared within 24 hours. It decreased.

[0048] (Example 5: Antifungal effect of Lactobacillus species) As fungi, dermatophytes and Candida were prepared. 10 mL of lactobacilli prepared in Example 1 Soy milk fermented liquid containing the genus *Cynamomum*, infused with 10 *Trichophyton* or *Candida*. 7 Contains 0.1 at a concentration of 0.1 cells / mL mL of bacterial solution was inoculated and allowed to react at 25°C. The number of viable bacteria in the inoculated bacteria was measured over 24 hours. Additionally, as a control, 10 mL of phosphorus solution with a concentration of 1 / 15 mol / L and pH 7.2 was used. 0.1 mL of bacterial solution was inoculated into an acid buffer, and the number of viable bacteria was measured over time at 25°C. Measurements were taken for 24 hours. As a result, as shown in Figure 4, the soy milk fermented liquid containing Lactobacillus was The prepared dermatophytes and Candida were reduced within 24 hours.

[0049] (Example 6: Antiviral effect of Lactobacillus species) As an enveloped virus, a culture medium of influenza virus type A (H1N1) was used. I intended to include it. Also, as a non-enveloped virus, norovirus (feline calicivirus) A culture medium was prepared. The virus culture medium was serially diluted 10 times with purified water. , 50% Tissue Culture Infectious Dose (TCID50:50% Tissue Culture In The antiviral test was conducted at room temperature according to the fectious dose. The experiment was conducted at the Japan Food Research Laboratories.

[0050] As a result, as shown in Figure 5, the soy milk fermented liquid containing Lactobacillus genus was used within 1 hour. It reduced the infectivity titer of the influenza virus. Also, as shown in Figure 6, Lactobacillus Soy milk fermented liquid containing the genus reduced the infectivity titer of norovirus within 24 hours.

[0051] (Example 7: Antifungal effect of Lactobacillus species) The unheat-treated mugwort fermented liquid obtained in Example 1 was spray-dried to extract Lactobacillus species. Dried bacterial cells were obtained. The dried bacterial cells were mixed with water until the amount of dried bacterial cells was 10 parts by weight. The Lactobacillus species were suspended in ricerin to obtain the suspension of Lactobacillus species according to Example 7. When the suspension was added to the dermatophytes and the colony-forming units (CFU) of the dermatophytes were measured, the results were as shown in the figure. As shown in 7, the Lactobacillus suspension killed the ringworm fungus within 24 hours.

[0052] The embodiments and examples described above are provided to facilitate understanding of the present invention. This invention is not intended to be interpreted as limiting. The invention will be made without departing from its spirit. The present invention may be modified / improved, and equivalents thereof are also included. That is, each embodiment And any modifications made to the examples by those skilled in the art are also valid as long as they retain the features of the present invention. This is included within the scope of the invention. For example, each element of each embodiment and example is exemplary. It is not limited to those examples and can be modified as appropriate. Furthermore, each embodiment and implementation The examples are illustrative, and partial substitution or combination of the configurations shown in different embodiments is possible. Needless to say, these are also included in the scope of the present invention insofar as they include the features of the present invention. .

Claims

1. An antimicrobial and antiviral agent comprising a fermented mugwort leaf extract or a spray-dried product thereof containing an effective amount of Lactobacillus lactic acid bacteria of the genus Lactobacillus that reduces Gram-positive bacteria, Gram-negative bacteria, fungi, and viruses, The Lactobacillus species includes Lactobacillus parafarraginis, Lactobacillus parabuchneri, Lactobacillus buchneri, and Lactobacillus harbinensis. Antibacterial and antiviral drugs.

2. The antibacterial and antiviral agent according to claim 1, wherein the Lactobacillus lactic acid bacteria are dead bacteria.

3. An antimicrobial and antiviral agent comprising a fermented mugwort leaf extract or a spray-dried product thereof containing an effective amount of secretion from Lactobacillus lactic acid bacteria of the genus Lactobacillus, which reduces Gram-positive bacteria, Gram-negative bacteria, fungi, and viruses, The Lactobacillus species includes Lactobacillus parafarraginis, Lactobacillus parabuchneri, Lactobacillus buchneri, and Lactobacillus harbinensis. Antibacterial and antiviral drugs.

4. The antibacterial and antiviral agent according to any one of claims 1 to 3, wherein the Gram-positive bacteria include Gram-positive cocci and Gram-positive bacilli.

5. The antibacterial and antiviral agent according to claim 4, wherein the Gram-positive cocci include Staphylococcus aureus and MRSA.

6. The antibacterial and antiviral agent according to claim 4, wherein the Gram-positive bacilli include Bacillus subtilis and Bacillus cereus.

7. The antibacterial and antiviral agent according to any one of claims 1 to 6, wherein the Gram-negative bacteria include Gram-negative cocci and Gram-negative bacilli.

8. The antibacterial and antiviral agent according to claim 7, wherein the Gram-negative cocci include Escherichia coli, Salmonella, Vibrio parahaemolyticus, and Klebsiella pneumoniae.

9. The antibacterial and antiviral agent according to claim 7, wherein the Gram-negative bacilli include Pseudomonas aeruginosa.

10. The antibacterial and antiviral agent according to any one of claims 1 to 9, wherein the fungus includes dermatophytes and Candida.

11. The antimicrobial and antiviral agent according to any one of claims 1 to 10, wherein the virus includes enveloped viruses and non-enveloped viruses.

12. The antibacterial and antiviral agent according to claim 11, wherein the enveloped virus includes the influenza virus.

13. The antibacterial and antiviral agent according to claim 11, wherein the non-enveloped virus includes norovirus.

14. An antibacterial and antiviral agent according to any one of claims 1 to 13, excluding an antiviral immune response enhancer.

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