Method for promoting enzymatic reaction

Abstract

The present invention is a method for promoting an enzymatic reaction, the method including reacting a substrate and an enzyme in the presence of (a) a sulfosuccinic acid branched alkyl ester having a C9-12 branched alkyl group, or a salt thereof.

Description

Method for promoting enzyme reaction

[0001] The present invention relates to a method for promoting an enzyme reaction, an enzyme reaction accelerator, an enzyme reaction accelerator composition, and a dishwasher detergent composition.

[0002] Enzymes such as lipases, amylases, and proteases are used in many industrial sectors, including cleaning, food processing, and papermaking.

[0003] Japanese Patent Laid-Open Publication No. 63-7789 discloses a method for producing fatty acids by treating fats and oils with lipase in a reverse micelle system consisting of an organic solvent, a surfactant, and a buffer solution or water. Japanese Patent Laid-Open Publication No. 2012-75378 discloses a method for enzymatic saccharification of woody biomass, including a powdering step (1) in which at least one type of woody biomass selected from wood chips and waste building materials is pulverized to obtain woody biomass powder, and a saccharification step (2) in which the woody biomass powder is hydrolyzed in the presence of a predetermined anionic surfactant and a hydrolytic enzyme to obtain sugar.

[0004] Enzymes are used in many cleaning applications. Japanese Patent Application Laid-Open Publication No. 2019-182911 discloses a liquid detergent composition for tableware and / or kitchen hard objects, containing (a) an alkyl sulfosuccinate ester or a salt thereof, the alkyl group of which has 5 to 18 carbon atoms; (b) one or more surfactants selected from semi-polar surfactants and amphoteric surfactants; and (c) an enzyme and water under specified conditions. Japanese Patent Application Laid-Open Publication No. 2020-100745 discloses a liquid dishwashing detergent composition containing an anionic surfactant (component (A)) containing one or more (a1) components selected from secondary alkane sulfonates, dialkyl sulfosuccinates, and compounds represented by a specific formula; an amine oxide surfactant (component (B)); a protease (component (C)); and an amylase (component (D)), wherein the mass ratio of component (A) to component (B) is 0.7 to 2.

[0005] Furthermore, with the recent widespread use of automatic dishwashers, many detergent compositions for dishwashers have been proposed. Examples of stains that adhere to tableware include oil stains, starch stains such as rice grains, protein stains such as eggs, and pigment stains such as tea. Among these, oil stains and starch stains are particularly difficult to remove, and washing in a dishwasher poses the problem of the stains reattaching to the dishes and causing glass tableware to become cloudy. Therefore, in order to enhance the detergency and anti-reattachment properties against the stains, various formulations have been proposed that combine, for example, types of active cleaning ingredients such as surfactants, enzymes, and chelating agents.

[0006] Japanese Patent Publication No. 4-72397 discloses a technology that combines a specific nonionic surfactant and a calcium-sequestering chelating agent with alkaline pullulanase and lipase having α-amylase activity to achieve high detergency. Japanese Patent Publication No. 2006-152287 also describes a technology that prevents clouding of glass tableware and improves the finish by combining a high concentration of chelating agent with a low-foaming nonionic surfactant. The aforementioned Japanese Patent Publication No. 2019-182911 discloses a liquid detergent composition containing a sulfosuccinate alkyl ester salt and an enzyme, and a cleaning method in which the composition is brought into direct contact with tableware stained with grease in a foamy state. The aforementioned Japanese Patent Publication No. 2020-100745 discloses that a composition containing a dialkyl sulfosuccinate, a protease, and an amylase has excellent detergency and defoaming properties.

[0007] Summary of the Invention When enzymes are used in various industrial fields, they are often reacted with substrates in aqueous media, necessitating a reaction field where water, substrate, and enzyme come into contact. However, while enzymes are hydrophilic, substrates are often hydrophobic, resulting in low affinity between the substrate and enzyme, making the enzymatic reaction difficult and preventing the expected enzymatic activity from being achieved. To address this issue, for example, methods have been used, such as using large amounts of aqueous solvent or using surfactants in combination. However, the former is uneconomical because it uses aqueous solvent unnecessary for the reaction, and the latter, while improving the affinity between the substrate and enzyme, results in excessive surfactant action on the enzyme, reducing the enzymatic activity itself. Therefore, there is a strong demand for technology that promotes the action of substrates and enzymes while maintaining high enzymatic activity in aqueous media.

[0008] The present invention provides a method for enhancing an enzymatic reaction, for example, an enzymatic reaction in an aqueous medium.

[0009] Furthermore, when it comes to dishwasher detergents, it is desirable to emulsify the oil adhering to dishes with a very small amount of surfactant to prevent foam from overflowing inside the dishwasher and compromising cleaning power. However, because the amount of surfactant is small, no technology has yet been found that combines high cleaning power with improved finish by preventing dirt from re-adhering to dishes.

[0010] Thus, a technology for dishwasher detergents that combines high detergency with prevention of redeposition on dishes using a minimal amount of surfactant is desired. However, conventional technologies have been insufficient in preventing the redeposition of oily and proteinaceous soils on dishes, resulting in the appearance of white spots on the surface of dishes after washing and drying, significantly impairing the finish. The inventors have clarified that the cause of this is that during the drainage process (transition from washing to rinsing), some oil droplets detached from the dishes remain in the circulation line specific to dishwashers. When the oil droplets are carried over into the dishwasher again during the rinsing process, the destabilized oil droplets coalesce and become larger in particle size due to dilution (decrease in surfactant concentration), temperature change (from high temperature to room temperature), and agitation halted during drainage, making them more likely to redeposit on dishes. Therefore, there has been a need for a technology for stabilizing the dispersion of oily soils that takes into account the rinsing process specific to dishwashers.

[0011] The present invention provides a detergent composition for dishwashers that has excellent detergency and excellent finish on tableware. In particular, the present invention provides a detergent composition for dishwashers that has excellent detergency on stubborn stains such as oil and starch, and excellent finish on tableware made of plastic or glass.

[0012] The present invention relates to a method for promoting an enzymatic reaction, which comprises reacting a substrate with an enzyme in the presence of (a) a branched alkyl sulfosuccinate ester having a branched alkyl group having from 9 to 12 carbon atoms or a salt thereof.

[0013] The present invention also relates to an enzyme reaction accelerator containing, as an active ingredient, (a) a sulfosuccinic acid branched alkyl ester having a branched alkyl group having from 9 to 12 carbon atoms or a salt thereof.

[0014] The present invention also relates to an enzyme reaction accelerator composition comprising (a) an enzyme reaction accelerator containing, as an active ingredient, a sulfosuccinic acid branched alkyl ester having a branched alkyl group having from 9 to 12 carbon atoms or a salt thereof [hereinafter referred to as component (a)], and optionally a surfactant other than component (a) [hereinafter referred to as component (b)], wherein, when component (b) is contained, the mass ratio (a) / (b) of the content of component (a) to the content of component (b) is 0.001 or more and 50 or less.

[0015] The present invention also relates to a dishwasher detergent composition containing (a) a sulfosuccinic acid branched alkyl ester having a branched alkyl group having from 9 to 12 carbon atoms or a salt thereof [hereinafter referred to as component (a)], and (d) an enzyme [hereinafter referred to as component (d)].

[0016] According to the present invention, there is provided a method for promoting an enzymatic reaction, for example, an enzymatic reaction in an aqueous medium.

[0017] Furthermore, the present invention can provide a detergent composition for dishwashers that has excellent detergency and excellent finish on tableware. In particular, the present invention can provide a detergent composition for dishwashers that has excellent detergency on stubborn stains such as oil and starch, and excellent finish on tableware made of plastic or glass.

[0018] Modes for Carrying Out the Invention [Method for Promoting an Enzyme Reaction] In the method for promoting an enzymatic reaction of the present invention, a substrate is reacted with an enzyme in the presence of component (a), preferably in the presence of component (a) and water.

[0019] Sulfosuccinate esters or salts thereof are known as components incorporated into detergent compositions for hard articles, as described in Patent Document 3, for example. However, it was not known in the art that compounds having a structure corresponding to component (a) of the present invention can significantly promote enzymatic reactions with various enzymes such as proteases. For example, as shown in the comparative examples described below, a compound having a structure similar to component (a), but with a branched alkyl group having 8 carbon atoms, does not exhibit the excellent effect of improving enzymatic reactions as component (a) of the present invention. In other words, even compounds having a structure similar to component (a) have significant differences in their effects on enzymes, which is an unexpected effect that those skilled in the art could not have predicted. Enzymes are used in various industrial fields, such as the cleaning field, and in such cases, they are often used in combination with other chemicals. The ability to promote enzymatic reactions and improve their effects leads to a reduction in the amounts of surfactants and solvents used, for example, and can reduce CO ２ The inventors believe that this will also contribute to the realization of a sustainable society in terms of reducing CO2 emissions.

[0020] The mechanism by which the enzymatic reaction is promoted by the present invention is presumed to be, but not limited to, the following: In the presence of component (a), the spreading and wetting of the reaction substrate proceeds with high efficiency, making it possible to promote the reactivity of the enzyme contained in water with the substrate. According to the present invention, the use of other surfactants and solvents that cause a decrease in enzyme activity can be minimized, thereby maintaining high enzyme activity and allowing the catalytic reaction by the enzyme to be sustained.

[0021] Component (a) is preferably a diester. That is, component (a) is preferably a di-branched alkyl sulfosuccinate ester or a salt thereof. Component (a) is preferably a branched alkyl sulfosuccinate ester having a branched alkyl group of 9 or 10 carbon atoms or a salt thereof. Component (a) is preferably a branched alkyl sulfosuccinate ester having a branched alkyl group of 10 carbon atoms or a salt thereof. Component (a) is preferably a di-branched alkyl sulfosuccinate ester or a salt thereof, wherein the two branched alkyl groups are each a branched alkyl group of 9 or 10 carbon atoms or a salt thereof. Component (a) is preferably a di-branched alkyl sulfosuccinate ester or a salt thereof, wherein the two branched alkyl groups are each a branched alkyl group of 10 carbon atoms or a salt thereof. The method for promoting an enzymatic reaction of the present invention includes methods for promoting an enzymatic reaction that combine these preferred component (a) with the features described below.

[0022] When component (a) is a salt, examples of the salt include alkali metal salts, alkanolamine salts, etc. The salt of component (a) is preferably an alkali metal salt or an alkanolamine salt, more preferably a salt selected from sodium salt, potassium salt, triethanolamine salt, diethanolamine salt, and monoethanolamine salt, and even more preferably a sodium salt.

[0023] Examples of the component (a) include compounds represented by the following formula 1.

[0024]

[0025] [In the formula, R １ , R ２ are each a branched alkyl group having 9 to 12 carbon atoms, and A １ O.A. ２ Each O is an alkyleneoxy group having 2 to 4 carbon atoms, x1 and x2 are the average number of moles added and are each a number of 0 to 10, and M is a cation.

[0026] R １ , R ２In the present invention, the branched chain hydrocarbon group includes a hydrocarbon residue obtained by removing a hydroxyl group from a secondary alcohol.

[0027] In the present invention, R １ , R ２ Among the branched chain hydrocarbon groups, the hydrocarbon chain with the largest number of carbon atoms, counting from the carbon atom bonded to the oxygen atom, is defined as the main chain, and the hydrocarbon chains branching from and bonded to the main chain are defined as side chains. When two or more main chains are possible, i.e., when there are two or more hydrocarbon chains with the largest number of carbon atoms (hereinafter also referred to as the longest hydrocarbon chains), the main chain is determined in the following order: 1. The side chain with the largest number of carbon atoms branching from the longest hydrocarbon chain is defined as the main chain. 2. Next, if the side chains branching from the longest hydrocarbon chain have the same number of carbon atoms, the side chain with the largest number of side chains branching from the longest hydrocarbon chain is defined as the main chain. 3. Next, if the number of side chains branching from the longest hydrocarbon chains are the same, the side chain with the side chain closest to the oxygen atom, counting from the carbon atom bonded to the oxygen atom, is defined as the main chain. 4. Next, if the positions of the carbon atoms with side chains closest to the oxygen atom are the same, the side chain with the largest number of carbon atoms closest to the oxygen atom is defined as the main chain. Note that when two or more longest hydrocarbon chains have the same symmetrical structure, either one may be defined as the main chain.

[0028] In formula 1, R １ , R ２ are each preferably a branched alkyl group selected from a branched nonyl group, a branched decyl group, and a branched dodecyl group, and more preferably a branched decyl group. The branched decyl group is preferably a 2-propylheptyl group.

[0029] In formula 1, A １ O.A. ２ O is an alkyleneoxy group having 2 to 4 carbon atoms, preferably 2 or 3 carbon atoms in view of lubricity to water. １ O.A. ２ Each represents the average number of moles of O added, and is 0 or more and 10 or less, and from the viewpoint of lubricity to water, is preferably 6 or less, more preferably 4 or less, even more preferably 2 or less, and 0 is even more preferred.

[0030] In formula 1, M is a cation. M is preferably a cation other than a hydrogen ion. Examples of M include alkali metal ions such as lithium ion, sodium ion, and potassium ion; alkaline earth metal ions such as calcium ion and barium ion; and organic ammonium ions such as triethanolammonium ion, diethanolammonium ion, monoethanolammonium ion, trimethylammonium ion, and monomethylammonium ion. From the viewpoint of dispersibility in water, M is preferably an alkali metal ion or an alkanolammonium ion, more preferably a sodium ion, potassium ion, triethanolammonium ion, diethanolammonium ion, or monoethanolammonium ion, and even more preferably a sodium ion.

[0031] The component (a) of the present invention is preferably a compound represented by the following formula 1-1: The compound of formula 1-1 is a compound in which x1 and x2 are each 0 in formula 1.

[0032]

[0033] [In the formula, R １ , R ２ are each a branched alkyl group having 9 to 12 carbon atoms, and M is a cation.] R in Formula 1-1 １ , R ２ Specific examples and preferred examples of M are the same as those of formula 1.

[0034] In Formula 1 or Formula 1-1, R １ , R ２ The method for preparing the compound in which R is the same is not particularly limited, but for example, it can be prepared by referring to the method described in U.S. Patent No. 2,028,091. １ , R ２As a method for preparing asymmetric compounds with different carbon atoms, for example, production can be carried out by referring to JP-A-58-24555. An alcohol having a predetermined number of carbon atoms to which an alkylene oxide is added can also be used as the raw material for component (a). Suitable alcohols for use in producing component (a) of the present invention include (1) primary alcohols such as 3,5,5-trimethylhexan-1-ol and 2-propylheptan-1-ol, and (2) secondary alcohols such as 5-nonanol and 2,6-dimethyl-4-heptanol.

[0035] In the present invention, it is preferable to bring an aqueous medium containing component (a), the enzyme, and water (hereinafter also referred to as the aqueous medium of the present invention) into contact with a substrate to react the substrate with the enzyme. The aqueous medium of the present invention may be a mixture of component (a), the enzyme, and water.

[0036] The content of component (a) in the aqueous medium of the present invention is preferably 0.001% by mass or more, more preferably 0.01% by mass or more, even more preferably 0.05% by mass, and preferably 10% by mass or less, more preferably 5% by mass or less, even more preferably 2% by mass or less, and still more preferably 1% by mass or less.

[0037] The aqueous medium of the present invention may optionally contain a surfactant other than component (a) [hereinafter referred to as component (b)], but its content is preferably low from the viewpoint of maintaining the effect of promoting the enzymatic reaction by component (a). The content of component (b) in the aqueous medium of the present invention is preferably 10% by mass or less, more preferably 5% by mass or less, even more preferably 3% by mass or less, and even more preferably 1% by mass or less. The aqueous medium of the present invention may not contain component (b). Note that when the aqueous medium of the present invention is applied to a substrate in the form of a foam, the adhesion efficiency of the enzyme with improved activity may be increased, thereby efficiently manifesting the effect of the enzyme. Therefore, when a surfactant is used in combination to adjust the foaming ability or foam characteristics of the aqueous medium, the content of component (b) in the aqueous medium of the present invention may exceed the above range.

[0038] The component (b) is preferably a surfactant that does not inhibit the enzymatic reaction. The component (b) can be appropriately selected optimally taking into consideration the embodiment of the enzymatic reaction promotion method of the present invention. Examples include one or more selected from (b1) semi-polar surfactants (hereinafter referred to as component (b1)), (b2) amphoteric surfactants (hereinafter referred to as component (b2)), (b3) anionic surfactants (hereinafter referred to as component (b3)), and (b4) nonionic surfactants (hereinafter referred to as component (b4)). The component (b1) includes an amine oxide surfactant having one or more, preferably one, alkyl group having from 8 to 14 carbon atoms. The component (b2) includes a sulfobetaine surfactant having one or more, preferably one, alkyl group having from 8 to 14 carbon atoms, and a carbobetaine surfactant having one or more, preferably one, alkyl group having from 8 to 14 carbon atoms. Examples of the (b3) component include alkyl sulfate salts in which the alkyl group has from 8 to 14 carbon atoms, alkylbenzene sulfonates in which the alkyl group has from 8 to 14 carbon atoms, polyoxyalkylene alkyl ether sulfates in which the alkyl group has from 8 to 14 carbon atoms (oxyalkylene having 2 or 3 carbon atoms, preferably oxyethylene, and the average number of moles of oxyalkylene added being from 0.5 to 5, preferably from 0.5 to 3), and fatty acid soaps in which the carbon atoms are from 8 to 14. Examples of the (b4) component include polyoxyalkylene alkyl ethers, for example, polyoxyalkylene alkyl ethers in which the alkyl group has from 8 to 14 carbon atoms (oxyalkylene having 2 or 3 carbon atoms, preferably oxyethylene, and the average number of moles of oxyalkylene added being from 3 to 50, preferably from 3 to 20), alkyl glycosides, for example, alkyl glycosides in which the alkyl group has from 8 to 14 carbon atoms (the average degree of condensation of the sugar skeleton, such as glucose, is from 1 to 5, preferably from 1 to 2), and alkyl glyceryl ethers, for example, alkyl glyceryl ethers in which the alkyl group has from 8 to 12 carbon atoms.

[0039] When the aqueous medium of the present invention contains the component (b), the mass ratio (a) / (b) of the content of the component (a) to the content of the component (b) is preferably 0.001 or more and 50 or less. (a) / (b) can further be selected from, for example, 0.01 or more and 40 or less.

[0040] Component (b) is preferably used in an amount within a range in which the total amount with component (a) does not affect the activity of the enzyme. In the present invention, for example, the mass ratio of the total mass of components (a) and (b) to the total mass of the enzyme [component (a) + component (b)] / enzyme is preferably 0.1 or more, more preferably 1 or more, and 200 or less, preferably less than 100, more preferably 50 or less, and even more preferably 25 or less.

[0041] The aqueous medium of the present invention contains water. The content of water in the aqueous medium of the present invention is preferably 50% by mass or more, more preferably 70% by mass or more. Water is used as the remainder of the aqueous medium.

[0042] The aqueous medium of the present invention may contain components other than component (a), enzyme, water, and optional component (b), such as a solvent and a hydrotropic agent.

[0043] Examples of the solvent (hereinafter referred to as component (c)) include (c1) monohydric alcohols having from 1 to 3 carbon atoms, (c2) polyhydric alcohols having from 2 to 4 carbon atoms, (c3) di- or trialkylene glycols having from 2 to 4 carbon atoms in the alkylene glycol unit, and (c4) monoalkoxy (methoxy, ethoxy, propoxy, butoxy), phenoxy, or benzooxy ethers of di- or tetraalkylene glycols having from 2 to 4 carbon atoms in the alkylene glycol unit. Component (c) is preferably a water-soluble organic solvent having from 2 to 10 carbon atoms, preferably from 8 to 20 carbon atoms. Here, the term "water-soluble organic solvent" refers to a solvent having an octanol / water partition coefficient (LogPow) of 3.5 or less. Specifically, the (c) component includes (c1) ethanol and isopropyl alcohol, (c2) ethylene glycol, propylene glycol, glycerin, and isoprene glycol, (c3) diethylene glycol and dipropylene glycol, and (c4) propylene glycol monomethyl ether, propylene glycol monoethyl ether, diethylene glycol monobutyl ether (also known as butyl diglycol), phenoxyethanol, phenoxytriethylene glycol, and phenoxyisopropanol. The (c) component is preferably a solvent selected from ethanol, propylene glycol, dipropylene glycol, diethylene glycol monobutyl ether, phenoxyethanol, phenyl glycol, and phenoxyisopropanol. The (c) component preferably has an alkoxy group and preferably further contains one or more selected from the above (c4). The aqueous medium of the present invention more preferably contains diethylene glycol monobutyl ether as the (c) component. The aqueous medium of the present invention can contain, for example, 0.1% by mass or more, further 0.2% by mass or more, and 10% by mass or less, further 8% by mass or less of the component (c).

[0044] The hydrotrope is preferably selected from alkylbenzenesulfonic acids having one to three alkyl groups with one to three carbon atoms and their salts. Specific examples include toluenesulfonic acid, xylenesulfonic acid, and cumenesulfonic acid, as well as salts thereof. The salts are preferably sodium, potassium, or magnesium salts. The hydrotrope is preferably p-toluenesulfonic acid or a salt thereof. The aqueous medium of the present invention may contain the hydrotrope in an amount of, for example, 0.1% by mass or more, preferably 0.2% by mass or more, and 10% by mass or less, more preferably 8% by mass or less, even more preferably 7% by mass or less, still more preferably 6% by mass or less, and even more preferably 5% by mass or less.

[0045] From the viewpoint of the effect of promoting the enzymatic reaction, the component (a) is preferably used such that the mass ratio of enzyme / component (a) is 0.001 or more, more preferably 0.02 or more, even more preferably 0.05 or more, and is 15 or less, even more preferably 9 or less, even more preferably 5 or less, and even more preferably 1 or less. In the present invention, the amount of the enzyme is the amount as enzyme protein.

[0046] Examples of the enzyme include protease, amylase, and lipase, and the enzyme is preferably one or more selected from protease, amylase, and lipase.

[0047] Preferred lipases include E.C. 3.1.1.3 triacylglycerol lipase, E.C. 3.1.1.13 cholesterol esterase, E.C. 3.1.23 monoacylglycerol lipase, and E.C. 3.1.1.34 lipoprotein lipase. The origin of the lipase is not limited, and examples include lipases derived from animals, plants, or microorganisms. Microbial lipases include those derived from the genus Rizopus, Aspergillus, Mucor, Pseudomonas, Geotrichum, Penicillium, and Candida. Examples of lipase that can be used include Lipase A "Amano" 6, Lipase AY "Amano" 30SD, Lipase GS "Amano" 250G, Lipase R "Amano", Lipase DF "Amano" 15, Lipase MER "Amano" (all manufactured by Amano Enzyme Co., Ltd.), Lipase (Nagase & Co., Ltd.), Lipase MY, Lipase OF, Lipase PL, Lipase PLC, Lipase QLM, Lipase QLC, and Phospholipase D (all manufactured by Meito Sangyo Co., Ltd.), Lipoprotein Lipase (manufactured by Oriental Yeast Co., Ltd.), Lipase (manufactured by Toyo Brewing Co., Ltd.), Lipex, Lipolase, and Lipase SP-225 (all manufactured by Novo), Lipase (manufactured by Gist), Lipase A, and Lipase B (all manufactured by Sapporo Breweries Ltd.). In the present invention, Lipex and Lipolase (both manufactured by Novo Pharmaceuticals) are preferred.

[0048] Examples of proteases include proteases that can function in neutral or alkaline aqueous solutions. Specific examples of preferred proteases include the alkaline proteases described in WO 99 / 018218, preferably those in which 70% or more of the amino acid sequence shown in SEQ ID NO: 1 or 2 is conserved, and the alkaline proteases described in JP 5-25492 A, preferably alkaline protease K-16 or alkaline protease K-14. Other examples include proteases produced by Bacillus subtilisins sold under the trade names Savinase (registered trademark), Cannase (registered trademark), Evalase (registered trademark), Alcalase (registered trademark), Polarzyme (registered trademark), and Esperase (registered trademark) manufactured by Novozymes, and proteases or variants thereof supplied under the trade names FN2 (registered trademark), FN3 (registered trademark), and FN4 (registered trademark), Prafect (registered trademark), and Prafect Prime (registered trademark) manufactured by DuPont. Among these, enzymes in which 80% or more of the amino acid sequence shown in SEQ ID NO: 1 or 2 described in WO 99 / 018218 is conserved, such as Savinase, Evalase, Alcalase, and Progress (manufactured by Novozymes), and Prafect and Prafect Prime (manufactured by DuPont), are more preferred.

[0049] Examples of amylases include those derived from bacteria such as Bacillus subtilis Marburg, Bacillus subtilis natto, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus cereus, Bacillus macerans, Pseudomonas stutzeri, and Klebsiella aerogenes, actinomycetes such as Streptomyces griseus, and those derived from bacteria such as Aspergillus oryzae and Aspergillus niger. Amylases obtained from many organisms, such as fungi such as Amylase niger, seeds of grasses and legumes, and the digestive glands of animals such as humans and pigs, can be used. The amylase used in the present invention can be obtained by inoculating the above-mentioned microorganisms or their mutants, or host cells transformed with a recombinant vector containing a DNA sequence encoding these enzymes or their mutants, into a medium containing an assimilable carbon source, a nitrogen source, and other essential nutrients, culturing them according to standard methods, and following general enzyme isolation and purification methods. The enzyme solution thus obtained can be used as is, or it can be further purified, crystallized, or formulated into a powder or liquid by known methods. The amylase used in the present invention is preferably α-amylase. Commercially available amylases that can be used include those under the trade names Rapidase (Gistbrokers), Termamyl, Duramyl, and Steinzyme (Novozymes Japan), Amplify (Novozymes), and Plaster ST and Plaster OxAm (Genencor International).

[0050] The enzyme described above can be used as component (d) in the dishwasher detergent composition of the present invention, which will be described later.

[0051] The substrate can be selected depending on the enzyme, but is preferably one or more selected from proteins, starches, and lipids.

[0052] The reaction conditions between the substrate and the enzyme can be appropriately set in consideration of the amount of the substrate, the activity of the enzyme, etc. For example, the temperature, pH, composition, amount used, contact time with the substrate, etc. of the aqueous medium of the present invention can be set in consideration of the optimum temperature, optimum pH, enzymatic activity, etc. of the enzyme.

[0053] The conditions for contacting the aqueous medium of the present invention with the substrate are not limited, but the temperature of the aqueous medium of the present invention can be selected from, for example, 0° C. or higher, further 10° C. or higher, further 30° C. or higher, further 35° C. or higher, and 100° C. or lower, further 80° C. or lower, further 70° C. or lower, and further 50° C. or lower. The contact time between the aqueous medium of the present invention and the substrate can be selected, for example, from 0.5 minutes or higher, further 1.0 minute or higher.

[0054] The present invention can be directed to hydrolysis reactions including, for example, protein hydrolysis reactions with proteases, starch hydrolysis reactions with amylases, lipid hydrolysis reactions with lipases, and combinations thereof.

[0055] In the present invention, from the viewpoint of increasing the adhesion efficiency of the enzyme with improved activity and thereby efficiently exerting the effect of the enzyme, it is preferable to foam the aqueous medium of the present invention and contact the substrate with the foam obtained by foaming the aqueous medium of the present invention.

[0056] Furthermore, since substrates present in the environment often exist in a complex state, such as proteins, starches, and lipids, two or more enzymes can also be used. When using two or more enzymes, they can be reacted with the substrate separately, or they can be combined into a complex enzyme and reacted with the substrate. In either case, sufficient care must be taken to prevent inactivation due to reaction between the enzymes. Furthermore, for complex substrates, multiple enzymes with different reactivities to the substrate can be allowed to act in stages. These methods can also be combined.

[0057] As an example, when the enzyme includes lipase, the aqueous medium of the present invention may be an aqueous medium containing 0.01% by mass or more, further 0.1% by mass or more, and 2.0% by mass or less, and further 1.0% by mass or less of component (a), 0.005% by mass or more, further 0.01% by mass or more, and 1.0% by mass or less, and further 0.5% by mass or less of lipase, and water.

[0058] As another example, when the enzyme includes a protease, the aqueous medium of the present invention may be an aqueous medium containing 0.001% by mass or more, further 0.005% by mass or more, and 5.0% by mass, further 1.0% by mass, further 0.1% by mass or less, and further 0.05% by mass or less of component (a), 0.00001% by mass or more, further 0.0001% by mass or more, and 1.0% by mass or less, further 0.5% by mass or less, further 0.1% by mass or less, and further 0.01% by mass or less of protease, and water.

[0059] From the viewpoint of the enzyme reaction promoting effect, the hardness of the aqueous medium of the present invention is preferably 0° DH or more, more preferably 4° DH or more, and preferably 20° DH or less, more preferably 10° DH or less, and even more preferably 8° DH or less, on the German hardness scale. However, when the content of component (a) in the aqueous medium of the present invention is low, for example, 0.02% by mass or less, the presence of the hardness component increases the impact of the loss of the effective amount of component (a) that promotes the spreading and wetting of the reaction substrate, so the hardness of the aqueous medium of the present invention is preferably low, for example, 0° DH.

[0060] In the present invention, the substrate may be present in soil attached to an article. In the present invention, the substrate may be present in soil attached to the hard surface of an article having a hard surface. In addition, in the present invention, the substrate may be present in soil attached to fibers (such as clothing). When a substrate is present in soil attached to an article, for example, an article having a hard surface or fibers, contacting the aqueous medium of the present invention with the soil promotes an enzymatic reaction in situ. Since substrates such as proteins, starches, and lipids are often the soil itself, it is believed that the method of the present invention also improves the soil removal effect.

[0061] An example of the enzymatic reaction promoting method of the present invention is a method of reacting a lipid with a lipase in the presence of component (a). Another example of the enzymatic reaction promoting method of the present invention is a method of contacting an aqueous medium containing component (a), lipase, and water with a lipid to react with the lipase.

[0062] [Enzyme Reaction Accelerator and Enzyme Reaction Accelerator Composition] The present invention was discovered based on the discovery that an enzymatic reaction is accelerated by component (a). The present invention relates to an enzymatic reaction accelerator containing component (a) as an active ingredient. The enzymatic reaction accelerator of the present invention may consist essentially of component (a). The present invention relates to an enzymatic reaction accelerator composition containing component (a) and, optionally, component (b), wherein, when component (b) is contained, the mass ratio of the content of component (a) to the content of component (b), (a) / (b), is 0.001 or more and 50 or less. The present invention relates to the use of component (a) as an enzymatic reaction accelerator. The present invention relates to the use of a composition containing component (a) and, optionally, component (b), wherein, when component (b) is contained, the mass ratio of the content of component (a) to the content of component (b), (a) / (b), is 0.001 or more and 50 or less, as an enzymatic reaction accelerator composition. The enzymatic reaction accelerator, enzymatic reaction accelerator composition, and use of the present invention can be appropriately applied to the enzymatic reaction accelerator, enzymatic reaction accelerator composition, and use of the present invention. Specific examples and preferred examples of component (a), component (b), enzyme, substrate, etc. in the enzymatic reaction accelerator, enzymatic reaction accelerator composition, and use of the present invention are also the same as those in the enzymatic reaction accelerator method of the present invention. The mass ratio (a) / (b) can be selected, for example, from 0.001 or more, preferably 0.01 or more, and preferably 50 or less, more preferably 40 or less.

[0063] [Dishwasher Detergent Composition] The dishwasher detergent composition of the present invention contains (a) a branched alkyl sulfosuccinate ester having a branched alkyl group having from 9 to 12 carbon atoms [hereinafter referred to as component (a)], and (d) an enzyme [hereinafter referred to as component (d)].

[0064] The mechanism by which the dishwasher detergent composition of the present invention improves detergency and the finish of dishes is not clear, but is presumed to be as follows. It is believed that the dishwasher detergent composition of the present invention, containing components (a) and (d), exhibits cleaning effects by removing dirt and preventing redeposition. However, the dishwasher detergent composition of the present invention is not limited to this mechanism of action.

[0065] <Component (a)> The component (a) is a branched alkyl sulfosuccinate ester having a branched alkyl group having from 9 to 12 carbon atoms. Specific examples and preferred embodiments of the component (a) can be selected from those described in connection with the method for promoting an enzymatic reaction of the present invention.

[0066] In the dishwasher detergent composition of the present invention, the branched alkyl group of component (a) is preferably a branched alkyl group having a main chain of 6 or 7 carbon atoms and one or more side chains, with the total number of carbon atoms in the side chains being 2 or more and 4 or less. In the dishwasher detergent composition of the present invention, the branched alkyl group of component (a) is preferably a branched alkyl group selected from a 2-propylheptyl group, a 4-methyl-2-propylhexyl group, a 5-methyl-2-propylhexyl group, and a 3,5,5-trimethylhexyl group, and more preferably a branched alkyl group selected from a 2-propylheptyl group and a 3,5,5-trimethylhexyl group.

[0067] Examples of the component (a) of the dishwasher detergent composition of the present invention include the compound represented by the formula 1 above and further the compound represented by the formula 1-1 above.

[0068] In the dishwasher detergent composition of the present invention, in the formula 1 or 1-1, R １ , R ２ In each of the branched-chain alkyl groups, the total number of carbon atoms constituting the side chains may be the same or different, and from the viewpoint of detergency, is preferably 3. In the present invention, the total number of carbon atoms constituting the side chains refers to the total number of carbon atoms in all side chains other than the main chain in one branched-chain alkyl group, and when there are multiple side chains, it refers to the total number of carbon atoms in all side chains.

[0069] In the dishwasher detergent composition of the present invention, in the formula 1 or 1-1, R１ , R ２ The number of side chains may be the same or different, and from the viewpoint of detergency, is 1 or more, preferably 3 or less, more preferably 2 or less. In the present invention, the number of side chains refers to the number of side chains branching from the main chain, and the number of side chains does not change even if a side chain has a side chain branching from the side chain. However, from the viewpoint of detergency, a side chain may have a side chain branching from the side chain.

[0070] In the dishwasher detergent composition of the present invention, in the formula 1 or 1-1, R １ , R ２ The number of branched carbon atoms may be the same or different, and from the viewpoint of detergency, is 1 or more, and preferably 3 or less, and even more preferably 2 or less. In the present invention, the number of branched carbon atoms means the total number of tertiary carbon atoms and quaternary carbon atoms in the branched-chain hydrocarbon group.

[0071] In the dishwasher detergent composition of the present invention, in the formula 1 or 1-1, R １ , R ２ A preferred embodiment of R １ , R ２ The total number of carbon atoms in the branched chain hydrocarbon groups is independently 9 or more and 11 or less, and further 9 or 10; the number of carbon atoms in the main chain is independently 6 or 7; the number of carbon atoms constituting the side chains is independently 1 or more and 3 or less; and the number of side chains is independently 1.

[0072] In the dishwasher detergent composition of the present invention, in the formula 1 or 1-1, R １ , R ２ The branched alkyl groups may be the same or different, and specific examples include branched alkyl groups selected from a 2-propylheptyl group, a 4-methyl 2-propylhexyl group, a 5-methyl 2-propylhexyl group, and a 3,5,5-trimethylhexyl group.

[0073] In the dishwasher detergent composition of the present invention, in the formula 1 or 1-1, A １ O.A. ２Each O is independently an alkyleneoxy group having 2 or more carbon atoms and 4 or less, preferably 3 or less carbon atoms. In the dishwasher detergent composition of the present invention, in Formula 1 or Formula 1-1, x1 and x2 represent the average number of moles added, and from the viewpoint of detergency, each independently is 0 or more and 6 or less, preferably 4 or less, more preferably 2 or less, and even more preferably 0. Furthermore, from the viewpoint of detergency, x1+x2 is preferably 0 or more and 12 or less, more preferably 6 or less, even more preferably 3 or less, and still more preferably 0.

[0074] In the dishwasher detergent composition of the present invention, M in Formula 1 or Formula 1-1 represents a hydrogen ion, an inorganic cation such as a sodium ion, an ammonium ion, a potassium ion, or a magnesium ion, or an organic cation such as a monoethanolammonium ion, a diethanolammonium ion, a triethanolammonium ion, or a morpholinium ion, and is preferably an inorganic cation selected from the group consisting of a sodium ion, an ammonium ion, a potassium ion, and a magnesium ion.

[0075] <Component (d)> The component (d) is an enzyme. Examples of the component (d) include one or more enzymes selected from amylase, protease, and lipase. From the viewpoint of cleaning performance, the component (d) is preferably two or more enzymes selected from amylase, protease, and lipase, and more preferably two or more enzymes including at least amylase and protease. Specific examples and preferred embodiments of the component (d) can be selected from those described in the enzymatic reaction promotion method of the present invention.

[0076] <Composition and other components of the dishwasher detergent composition of the present invention> The dishwasher detergent composition of the present invention may contain component (a) in an amount of, for example, 0.01% by mass or more, further 0.05% by mass or more, or even 0.1% by mass or more from the viewpoint of detergency, and in an amount of, for example, 5% by mass or less, further 3% by mass or less, further 2% by mass or less, or even 1% by mass or less from the viewpoint of storage stability. Note that, in the present invention, descriptions of the amount of component (a) (such as mass%) are based on the mass of the compound converted into a sodium salt, for example, the mass when M in Formula 1 above is assumed to be sodium.

[0077] From the viewpoints of detergency and cost, the dishwashing detergent composition of the present invention may contain component (d) in an amount of preferably 0.001% by mass or more, more preferably 0.005% by mass or more, even more preferably 0.01% by mass or more, and preferably 15% by mass or less, more preferably 9% by mass or less, and even more preferably 5% by mass or less, as enzyme protein. Regarding component (d), the Lowry method may be used as a method for quantifying the enzyme protein.

[0078] In the dishwasher detergent composition of the present invention, the mass ratio (a) / (d) of the content of component (a) to the content of component (d) is, from the viewpoint of cleaning performance, preferably 0.1 or more, more preferably 1 or more, even more preferably 10 or more, and preferably 50 or less, more preferably 40 or less, even more preferably 30 or less. The content of component (d) in the dishwasher detergent composition of the present invention is expressed as the mass of the enzyme protein.

[0079] The dishwasher detergent composition of the present invention may optionally contain a surfactant other than component (a) [the aforementioned component (b)]. Specific examples and preferred embodiments of component (b) can be selected from those described in the enzymatic reaction promotion method of the present invention. Examples of component (b) include amphoteric surfactants [the aforementioned component (b2)], anionic surfactants (excluding component (a)) [the aforementioned component (b3)], and nonionic surfactants [the aforementioned component (b4)].

[0080] The content of component (a) in all surfactants contained in the dishwasher detergent composition of the present invention may be, from the viewpoint of cleaning performance, for example, 5% by mass or more, further 8% by mass or more, or even 10% by mass or more, and for example, 100% by mass or less, further 50% by mass or less, or even 30% by mass or less.

[0081] When the dishwasher detergent composition of the present invention contains an anionic surfactant other than component (a), the content of component (a) in all the anionic surfactants contained in the dishwasher detergent composition of the present invention is, from the viewpoint of achieving both detergency and foam-suppressing property, for example, 8% by mass or more, preferably 50% by mass or more, more preferably 90% by mass or more, and 100% by mass or less. The content of component (a) in all the anionic surfactants may be 100% by mass.

[0082] <Component (b4)> The dishwasher detergent composition of the present invention may optionally contain (b4) a nonionic surfactant [hereinafter referred to as component (b4)]. Component (b4) is a preferred component from the viewpoints of storage stability and detergency.

[0083] In the dishwasher detergent composition of the present invention, component (b4) is preferably one or more nonionic surfactants selected from alkyl glycosides, alkyl glyceryl ethers, and polyoxyalkylene alkyl ethers. The alkyl glycoside preferably has an alkyl group with at least 8 carbon atoms and preferably 14 or less, more preferably 12 or less, and even more preferably 10 or less. The alkyl glyceryl ether preferably has an alkyl group with at least 8 carbon atoms and preferably 14 or less, more preferably 12 or less, and even more preferably 10 or less. The polyoxyalkylene alkyl ether preferably has an alkyl group with at least 8 carbon atoms and preferably 14 or less, more preferably 12 or less, and even more preferably 10 or less, and the average number of moles of oxyalkylene groups, more preferably oxyethylene groups, added is preferably 3 or more and preferably 20 or less, and even more preferably 10 or less. Component (b4) is preferably a nonionic surfactant in which the alkyl group is derived from a secondary alcohol, and for example, a polyoxyalkylene alkyl ether in which an average number of moles of oxyethylene groups added to a secondary alcohol is 1 to 10 is preferred. The component (b4) is preferably a foam-suppressing component.

[0084] The dishwasher detergent composition of the present invention may contain the component (b4) in an amount of preferably 0.05% by mass or more, more preferably 0.1% by mass or more, and even more preferably 0.3% by mass or more, from the viewpoints of storage stability and detergency, and preferably 5% by mass or less, more preferably 3% by mass or less, and even more preferably 1.0% by mass or less, from the viewpoint of foam-inhibiting ability.

[0085] The dishwasher detergent composition of the present invention may contain a foam inhibitor. Examples of the foam inhibitor include polymeric compounds such as silicone and polypropylene glycol, and hydrophobic silica particles. The foam inhibitor may be, for example, an oil compound containing a silicone oil and hydrophobic silica fine powder. The foam inhibitor may also be selected from compounds that function as oils or solvents, and may be polymeric or low-molecular-weight compounds. Polymeric compounds are preferred, and polypropylene glycol is more preferred. From the viewpoint of foam inhibitory properties, the weight-average molecular weight of polypropylene glycol is preferably 1,000 or more, more preferably 2,000 or more, and even more preferably 3,000 or more. From the viewpoint of blend stability, it is preferably 10,000 or less, more preferably 8,000 or less, and even more preferably 5,000 or less. The weight-average molecular weight of polypropylene glycol is calculated based on the hydroxyl value (mg KOH / g). Specifically, the weight-average molecular weight of polypropylene glycol can be calculated by (m x 1000 x 56 / OHV). Here, m is the number of hydroxyl groups in the polypropylene glycol, and when the polypropylene glycol is a diol type, m is 2, and when it is a triol type, m is 3. Furthermore, OHV (mgKOH / g) is the hydroxyl value measured in accordance with JIS K 0070.

[0086] The dishwasher detergent composition of the present invention may contain a foam inhibitor in an amount of preferably 0.01% by mass or more, more preferably 0.05% by mass or more, and even more preferably 0.1% by mass or more from the viewpoint of storage stability, and in an amount of preferably 5% by mass or less, more preferably 4% by mass or less, and even more preferably 3% by mass or less from the viewpoint of foam suppression.

[0087] The dishwasher detergent composition of the present invention may contain a chelating agent. Examples of the chelating agent that can be used include polycarboxylic acids or salts thereof, such as citric acid, malic acid, tartaric acid, succinic acid, methylglycine diacetate, glutamic acid diacetate, hydroxyethyliminodiacetic acid, ethylenediaminedisuccinic acid, nitrilotriacetic acid, 1,3-propanediaminetriacetic acid, 1,3-diamino-2-hydroxypropanetetraacetic acid, glycol ether diaminetetraacetic acid, ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, triethylenetetraaminehexaacetic acid, dihydroxyethylglycine, and hydroxyethylethylenediaminedicarboxymethylglutamic acid, and tripolyphosphoric acid or salts thereof. Examples of the salt of the chelating agent include alkali metal salts such as sodium salts and potassium salts. The chelating agent is preferably at least one selected from citric acid, malic acid, ethylenediaminetetraacetic acid, methylglycine diacetic acid, glutamic acid diacetic acid, tripolyphosphate, and salts thereof, and more preferably at least one selected from citric acid, trisodium citrate, glutamic acid diacetic acid, and salts thereof.

[0088] When the dishwasher detergent composition of the present invention contains a chelating agent, the composition may contain the chelating agent in an amount of, for example, 2% by mass or more, further 5% by mass or more, further 10% by mass or more, and 30% by mass or less, further 25% by mass or less, or further 20% by mass or less.

[0089] The dishwashing detergent composition of the present invention may optionally contain a thickener such as xanthan gum, an antifoaming agent, a bleaching agent, and the like.

[0090] The dishwasher detergent composition of the present invention may be either a liquid detergent composition or a powder detergent composition. When the dishwasher detergent composition of the present invention is a liquid, the detergent composition may contain water. The water may be the remainder excluding component (a), component (d), component (b4), and other optional components. When the dishwasher detergent composition of the present invention is a powder, the detergent composition may contain Glauber's salt (sodium sulfate decahydrate). The Glauber's salt may be the remainder excluding component (a), component (d), component (b4), and other optional components.

[0091] When the dishwasher detergent composition of the present invention is a liquid, the dishwasher detergent composition of the present invention has a pH at 25° C. of preferably 5 or more, more preferably 6 or more, and even more preferably 7 or more, from the viewpoint of cleaning performance, and preferably 11 or less, more preferably 10 or less, and even more preferably 8 or less, from the viewpoint of safety when handling. The pH can be measured by a glass electrode method (the pH values ​​described below can also be measured by the same method).

[0092] When the dishwasher detergent composition of the present invention is a liquid, the viscosity of the dishwasher detergent composition of the present invention at 25°C is preferably 10 mPa s or more, more preferably 50 mPa s or more, and even more preferably 100 mPa s or more, from the viewpoint of ease of dispensing the detergent, and is preferably 5,000 mPa s or less, more preferably 2,500 mPa s or less, from the viewpoint of suppressing residual detergent. This viscosity is a value measured with a B-type viscometer (rotor No. 2, 6 rpm, measurement time 1 minute, liquid temperature 25°C).

[0093] When the dishwasher detergent composition of the present invention is in the form of a powder, the dishwasher detergent composition of the present invention may contain an alkaline agent, such as sodium carbonate, potassium carbonate, sodium bicarbonate, ethanolamine, sodium silicate, or potassium silicate.

[0094] The dishwasher detergent composition of the present invention can be used as a cleaning liquid diluted with water before use (hereinafter referred to as the cleaning liquid of the present invention). The cleaning liquid of the present invention may be a mixture containing the dishwasher detergent composition of the present invention and water.

[0095] The concentration (dilution concentration) of the dishwasher detergent composition of the present invention when used is preferably 0.01% by mass or more, more preferably 0.05% by mass or more, and even more preferably 0.1% by mass or more, from the viewpoint of cleaning performance, and is preferably 2% by mass or less, more preferably 1% by mass or less, and even more preferably 0.5% by mass or less, from the viewpoint of foaming.

[0096] The concentration of component (a) when using the dishwasher detergent composition of the present invention is, from the viewpoint of detergency, preferably 1 ppm or more, more preferably 2 ppm or more, even more preferably 5 ppm or more, and from the same viewpoint, preferably 100 ppm or less, more preferably 50 ppm or less, even more preferably 30 ppm or less.

[0097] The concentration of component (d) in the dishwasher detergent composition of the present invention during use is preferably 0.001 ppm or more, more preferably 0.005 ppm or more, and even more preferably 0.01 ppm or more from the viewpoint of detergency, and from the same viewpoint, is preferably 5 ppm or less, more preferably 2 ppm or less, and even more preferably 1 ppm or less. The mass of component (d) is the mass in terms of enzyme protein.

[0098] The dishwasher detergent composition of the present invention can be used as a cleaning liquid suitably diluted with water or the like, and the pH of the cleaning liquid obtained by diluting the dishwasher detergent composition of the present invention at 25°C is preferably 5 or more, more preferably 6 or more, even more preferably 6.5 or more, and preferably 12 or less, more preferably 11.5 or less, and even more preferably 11 or less. For example, the pH of a mixture of 0.2 mass% of the dishwasher detergent composition of the present invention and water at 25°C is preferably 5 or more, more preferably 6 or more, even more preferably 6.5 or more, and preferably 12 or less, more preferably 11.5 or less, and even more preferably 11 or less.

[0099] [Method for washing dishes] The dishwasher detergent composition of the present invention can be used in dishwashers, and further in automatic dishwashers, and is further suitable for use in commercial dishwashers. Tableware includes cooking utensils, cleaning utensils, etc. When washing with a dishwasher, and further in a commercial dishwasher, the dishwasher detergent composition of the present invention is used as a cleaning liquid mixed with water. In this case, a fixed amount of the composition is arbitrarily transferred into the dishwasher by a supply device, and an appropriate concentration of the cleaning liquid is maintained.

[0100] The present invention provides a cleaning liquid obtained by diluting the dishwasher detergent composition of the present invention with water, and a cleaning method for washing dishes in a dishwasher using the cleaning liquid. This cleaning method may include the steps of diluting the dishwasher detergent composition of the present invention with water to prepare a cleaning liquid, and washing dishes in a dishwasher and further in an automatic dishwasher using the cleaning liquid. In this step of preparing the cleaning liquid, the dishwasher detergent composition of the present invention, component (a), and component (d) are diluted so that the concentrations of the dishwasher detergent composition of the present invention, component (a), and component (d) in the cleaning liquid are respectively the concentrations at which the dishwasher detergent composition of the present invention will be used. The pH range of the cleaning liquid at 25°C is the same as the preferred pH range at 25°C of the cleaning liquid obtained by diluting the dishwasher detergent composition of the present invention.

[0101] The washing temperature of the washing liquid is preferably 30° C. or higher, more preferably 40° C. or higher, from the viewpoint of productivity and cleaning performance, and preferably 80° C. or lower, more preferably 70° C. or lower, from the viewpoint of preventing damage to the base of the tableware. After the tableware has been washed, it is usually rinsed in the same dishwasher, or even an automatic dishwasher, with water, warm water, or hot water, for example, hot water, preferably 30° C. or higher, more preferably 40° C. or higher, and preferably 80° C. or lower, for 5 to 40 minutes. In a dishwasher, the washing liquid is usually circulated by a pump and reused repeatedly.

[0102] The dishwasher detergent composition of the present invention is used for washing tableware in a dishwasher. Examples of tableware include items used to hold food, such as plates, bowls, and cups, and items used to serve food, such as chopsticks, forks, knives, and spoons. In the present invention, tableware may also refer to items that come into contact with food and their parts, such as storage containers (e.g., Tupperware and bottles), cooking utensils (e.g., knives, cutting boards, pots, frying pans, and fish grills), and cooking appliances (e.g., food processors and mixers), which can be washed in a dishwasher. Examples of materials for tableware include plastics (including silicone resins), metals, ceramics, wood, and combinations thereof. The dishwasher detergent composition of the present invention is suitable for washing tableware with hydrophobic surfaces, such as plastic tableware. Cleaning using the dishwasher detergent composition of the present invention is similar to cleaning using a conventional dishwasher.

[0103] [Aspects of the present invention] The following are examples of aspects of the present invention. The details described in the enzymatic reaction promotion method and dishwasher detergent composition of the present invention can be applied to these aspects, with modifications as necessary. Furthermore, the description of each aspect can be applied to other aspects, with modifications as necessary. <1> A method for promoting an enzymatic reaction, comprising reacting a substrate with an enzyme in the presence of (a) a sulfosuccinic acid branched alkyl ester having a branched alkyl group having from 9 to 12 carbon atoms, or a salt thereof [hereinafter referred to as component (a)].

[0104] <2> The method for promoting an enzyme reaction according to <1>, wherein the component (a) is a branched alkyl sulfosuccinate ester having a branched alkyl group of 9 or 10 carbon atoms or a salt thereof.

[0105] <3> The method for promoting an enzyme reaction according to <1> or <2>, wherein the component (a) is a di-branched alkyl sulfosuccinate or a salt thereof, in which the two branched alkyl groups are each a branched alkyl group having 9 or 10 carbon atoms.

[0106] <4> The method for promoting an enzyme reaction according to any one of <1> to <3>, wherein the branched-chain alkyl group of the component (a) is a 2-propylheptyl group.

[0107] <5> The method for promoting an enzyme reaction according to any one of <1> to <4>, wherein the component (a) is a branched alkyl sulfosuccinate ester represented by the following formula 1 or a salt thereof:

[0108]

[0109] [In the formula, R １ , R ２ are each a branched alkyl group having 9 to 12 carbon atoms, and A １ O.A. ２ Each O is an alkyleneoxy group having 2 to 4 carbon atoms, x1 and x2 are the average number of moles added and are each a number of 0 to 10, and M is a cation.

[0110] <6> In formula 1, R １ , R ２ and each represent a branched-chain alkyl group selected from a branched-chain nonyl group, a branched-chain decyl group, and a branched-chain dodecyl group, preferably a branched-chain decyl group.

[0111] <7> In formula 1, A １ O.A. ２ The method for promoting an enzyme reaction according to <5> or <6>, wherein O is an alkyleneoxy group having 2 to 4 carbon atoms, preferably 2 or 3 carbon atoms.

[0112] <8> The method for promoting an enzymatic reaction according to any one of <5> to <7>, wherein in formula 1, x1 and x2 are each a number of 0 or more and 10 or less, preferably 6 or less, more preferably 4 or less, even more preferably 2 or less, and still more preferably 0.

[0113] <9> The method for promoting an enzyme reaction according to any one of <5> to <8>, wherein in formula 1, M is an alkali metal ion or an alkanolammonium ion, preferably a sodium ion, a potassium ion, a triethanolammonium ion, a diethanolammonium ion, or a monoethanolammonium ion, and more preferably a sodium ion.

[0114] <10> The method for promoting an enzyme reaction according to any one of <1> to <9>, wherein an aqueous medium containing the component (a), the enzyme, and water is brought into contact with a substrate to react with the enzyme.

[0115] <11> The method for promoting an enzymatic reaction according to <10>, wherein the content of the component (a) in the aqueous medium is preferably 0.001% by mass or more, more preferably 0.01% by mass or more, even more preferably 0.05% by mass, and preferably 10% by mass or less, more preferably 5% by mass or less, even more preferably 2% by mass or less, and still more preferably 1% by mass or less.

[0116] <12> The method for promoting an enzyme reaction according to <10> or <11>, wherein the content of the component (a) in the aqueous medium is 0.01% by mass or more and 2% by mass or less, and further 1% by mass or less.

[0117] <13> The method for promoting an enzyme reaction according to any one of <10> to <12>, wherein the aqueous medium optionally contains a surfactant other than the component (a) [hereinafter referred to as the component (b)], and the content of the component (b) in the aqueous medium is 5.0 mass% or less.

[0118] <14> The method for promoting an enzyme reaction according to <13>, wherein the content of the component (b) in the aqueous medium is 3.0% by mass or less.

[0119] <15> The method for promoting an enzyme reaction according to <13> or <14>, wherein the component (b) is one or more selected from the group consisting of (b1) a semi-polar surfactant (hereinafter referred to as the component (b1)), (b2) an amphoteric surfactant (hereinafter referred to as the component (b2)), (b3) anionic surfactant (hereinafter referred to as the component (b3)), and (b4) a nonionic surfactant (hereinafter referred to as the component (b4)).

[0120] <16> The method for promoting an enzyme reaction according to <15>, wherein the component (b1) is an amine oxide surfactant having one or more, preferably one, alkyl group having from 8 to 14 carbon atoms.

[0121] <17> The method for promoting an enzyme reaction according to <15> or <16>, wherein the component (b2) is an amphoteric surfactant selected from sulfobetaine surfactants having one or more, preferably one, alkyl group(s) having from 8 to 14 carbon atoms, and carbobetaine surfactants having one or more, preferably one, alkyl group(s) having from 8 to 14 carbon atoms.

[0122] <18> The method for promoting an enzyme reaction according to any one of <15> to <17>, wherein the component (b3) is an anionic surfactant selected from alkyl sulfate salts having from 8 to 14 carbon atoms in the alkyl group, alkylbenzene sulfonates having from 8 to 14 carbon atoms in the alkyl group, polyoxyalkylene alkyl ether sulfates having from 8 to 14 carbon atoms in the alkyl group (oxyalkylene having from 2 to 3 carbon atoms, preferably oxyethylene, and the average number of moles of oxyalkylene added being from 0.5 to 5, preferably from 0.5 to 3), and fatty acid soaps having from 8 to 14 carbon atoms.

[0123] <19> The method for promoting an enzymatic reaction according to any one of <13> to <18>, wherein the aqueous medium contains the component (b), and the mass ratio (a) / (b) of the content of the component (a) to the content of the component (b) is 0.001 or more, further 0.01 or more, and 50 or less, further 40 or less.

[0124] <20> The method for promoting an enzymatic reaction according to any one of <13> to <19>, wherein the aqueous medium contains component (b), and the mass ratio of the total mass of component (a) and component (b) to the total mass of the enzyme [component (a) + component (b)] / enzyme is preferably 0.1 or more, more preferably 1 or more, and is 200 or less, preferably less than 100, more preferably 50 or less.

[0125] <21> The method for promoting an enzyme reaction according to any one of <10> to <20>, wherein the water content in the aqueous medium is preferably 50% by mass or more, more preferably 70% by mass or more.

[0126] <22> The method for promoting an enzyme reaction according to any one of <10> to <21>, wherein the aqueous medium contains a solvent (hereinafter referred to as component (c)).

[0127] <23> The method for promoting an enzymatic reaction according to <22>, wherein the component (c) is at least one selected from the group consisting of (c1) a monohydric alcohol having from 1 to 3 carbon atoms, (c2) a polyhydric alcohol having from 2 to 4 carbon atoms, (c3) a di- or trialkylene glycol having from 2 to 4 carbon atoms in the alkylene glycol unit, and (c4) a monoalkoxy (alkoxy is methoxy, ethoxy, propoxy, or butoxy), phenoxy, and benzooxy ether of a di- or tetraalkylene glycol having from 2 to 4 carbon atoms in the alkylene glycol unit.

[0128] <24> The method for promoting an enzyme reaction according to <22> or <23>, wherein the aqueous medium contains, as the component (c), a water-soluble organic solvent having an octanol / water partition coefficient (Log Pow) of 3.5 or less.

[0129] <25> The method for promoting an enzymatic reaction according to any one of <22> to <24>, wherein the aqueous medium contains, as component (c), one or more selected from (c1) ethanol and isopropyl alcohol, (c2) ethylene glycol, propylene glycol, glycerin, and isoprene glycol, (c3) diethylene glycol and dipropylene glycol, and (c4) propylene glycol monomethyl ether, propylene glycol monoethyl ether, diethylene glycol monobutyl ether, phenoxyethanol, phenoxytriethylene glycol, and phenoxyisopropanol, preferably one or more selected from ethanol, propylene glycol, dipropylene glycol, diethylene glycol monobutyl ether, phenoxyethanol, phenyl glycol, and phenoxyisopropanol, more preferably diethylene glycol monobutyl ether.

[0130] <26> The method for promoting an enzyme reaction according to any one of <22> to <25>, wherein the component (c) is contained in an amount of 0.1% by mass or more, more preferably 0.2% by mass or more, and 10% by mass or less, more preferably 8% by mass or less.

[0131] <27> The method for promoting an enzymatic reaction according to any one of <10> to <26>, wherein the aqueous medium contains a hydrotrope agent, preferably one or more hydrotrope agents selected from alkylbenzenesulfonic acids having from 1 to 3 alkyl groups and salts thereof, more preferably one or more hydrotrope agents selected from toluenesulfonic acid, xylenesulfonic acid, cumenesulfonic acid, and salts thereof.

[0132] <28> The method for promoting an enzyme reaction according to any one of <10> to <27>, wherein the aqueous medium is brought into contact with the substrate in a foam state.

[0133] <29> The method for promoting an enzymatic reaction according to any one of <10> to <28>, wherein the temperature of the aqueous medium when contacting the aqueous medium with the substrate is selected from 0°C or higher, further 10°C or higher, further 30°C or higher, further 35°C or higher, and 100°C or lower, further 80°C or lower, further 70°C or lower, and further 50°C or lower, and the contact time is selected from 0.5 minutes or longer, further 1.0 minute or longer.

[0134] <30> The method for promoting an enzymatic reaction according to any one of <10> to <29>, wherein the aqueous medium contains a lipase as the enzyme, and the aqueous medium contains 0.01% by mass or more, further 0.1% by mass or more and 2.0% by mass or less, further 1.0% by mass or less of the component (a), 0.005% by mass or more, further 0.01% by mass or more and 1.0% by mass or less, further 0.5% by mass or less of the lipase, and water.

[0135] <31> The method for promoting an enzymatic reaction according to any one of <10> to <30>, wherein the aqueous medium contains a protease as the enzyme, and the aqueous medium contains 0.001% by mass or more, further 0.005% by mass or more, and 5.0% by mass, further 1.0% by mass, further 0.1% by mass or less, and further 0.05% by mass or less of the component (a); 0.00001% by mass or more, further 0.0001% by mass or more, and 1.0% by mass or less, further 0.5% by mass or less, further 0.1% by mass or less, and further 0.01% by mass or less of the protease; and water.

[0136] <32> The method for promoting an enzyme reaction according to any one of <10> to <31>, wherein the hardness of the aqueous medium is, on the German hardness scale, preferably 0° DH or more, more preferably 4° DH or more, and preferably 20° DH or less, more preferably 10° DH or less, and even more preferably 8° DH or less.

[0137] <33> The method for promoting an enzymatic reaction according to any one of <1> to <32>, wherein the component (a) is used in an enzyme / component (a) mass ratio of 0.001 or more, preferably 0.02 or more, more preferably 0.05 or more, and 15 or less, preferably 9 or less, more preferably 5 or less.

[0138] <34> The method for promoting an enzymatic reaction according to any one of <1> to <33>, wherein the enzyme is at least one enzyme selected from the group consisting of protease, amylase, and lipase.

[0139] <35> The method for promoting an enzymatic reaction according to <34>, wherein the lipase is one or more selected from E.C. 3.1.1.3 triacylglycerol lipase, E.C. 3.1.1.13 cholesterol esterase, E.C. 3.1.23 monoacylglycerol lipase, and E.C. 3.1.1.34 lipoprotein lipase.

[0140] <36> Lipases include Lipase A "Amano" 6, Lipase AY "Amano" 30SD, Lipase GS "Amano" 250G, Lipase R "Amano", Lipase DF "Amano" 15, Lipase MER "Amano" (all manufactured by Amano Enzyme Co., Ltd.), lipase (Nagase & Co., Ltd.), lipase MY, lipase OF, lipase PL, lipase PLC, lipase QLM, lipase QLC, and phospholipase D (all manufactured by Meito Sangyo Co., Ltd.). (manufactured by Oriental Yeast Co., Ltd.), lipoprotein lipase (manufactured by Toyo Brewing Co., Ltd.), Lipex, Lipolase, Lipase SP-225 (all manufactured by Novo Biosciences), Lipase (manufactured by Gist), Lipase A, Lipase B (all manufactured by Sapporo Breweries Ltd.), preferably Lipex or Lipolase.

[0141] <37> The protease is an alkaline protease described in WO 99 / 018218, preferably one in which 70% or more of the amino acid sequence shown in SEQ ID NO: 1 or 2 is conserved, or an alkaline protease described in JP-A-5-25492, preferably alkaline protease K-16 or alkaline protease K-14, or a protease produced by Bacillus subtilisins sold under the trade names Savinase (registered trademark), Cannase (registered trademark), Evalase (registered trademark), Alcalase (registered trademark), Polarzyme (registered trademark), and Esperase (registered trademark) manufactured by Novozymes. , or one or more proteases or variants thereof supplied under the trade names FN2 (registered trademark), FN3 (registered trademark), and FN4 (registered trademark), Prafect (registered trademark), and Prafect Prime (registered trademark), manufactured by DuPont; more preferably, one or more selected from enzymes in which 80% or more of the amino acid sequence shown in SEQ ID NO: 1 or 2 described in WO 99 / 018218 is conserved; Savinase, Evalase, Alcalase, and Progress (manufactured by Novozymes); and Prafect and Prafect Prime, manufactured by DuPont.

[0142] <38> Amylases produced by bacteria such as Bacillus subtilis Marburg, Bacillus subtilis natto, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus cereus, Bacillus macerans, Pseudomonas stutzeri, and Klebsiella aerogenes, actinomycetes such as Streptomyces griseus, and strains of bacteria such as Aspergillus oryzae and Aspergillus niger. The method for promoting an enzyme reaction according to any one of <34> to <37>, wherein the amylase is one or more amylases obtained from fungi such as Amylase of the family ...

[0143] <39> The method for promoting an enzyme reaction according to any one of <1> to <38>, wherein the substrate is at least one selected from the group consisting of protein, starch, and lipid.

[0144] <40> The method for promoting an enzyme reaction according to any one of <1> to <39>, wherein the substrate is two or more kinds of substrates, and further the substrates are two or more kinds of substrates selected from proteins, starches, and lipids, and the method comprises one or more of the following: (1) using two or more kinds of enzymes and reacting the enzymes separately with the substrate; (2) reacting two or more kinds of enzymes integrated in the form of a conjugated enzyme with the substrate; and (3) allowing a plurality of enzymes having different reactivities to the substrate to act on the substrate in a stepwise manner.

[0145] <41> The method for promoting an enzyme reaction according to any one of <1> to <40>, wherein the substrate is present in dirt attached to an article.

[0146] <42> The method for promoting an enzyme reaction according to any one of <1> to <41>, wherein the substrate is present in dirt attached to the hard surface of an article having a hard surface.

[0147] <43> The method for promoting an enzyme reaction according to any one of <1> to <41>, wherein the substrate is present in dirt attached to the fiber.

[0148] <44> (a) An enzyme reaction accelerator comprising, as an active ingredient, a sulfosuccinic acid branched alkyl ester having a branched alkyl group having from 9 to 12 carbon atoms or a salt thereof.

[0149] <45> An enzyme reaction accelerator composition comprising: (a) an enzyme reaction accelerator containing, as an active ingredient, a sulfosuccinic acid branched alkyl ester having a branched alkyl group having from 9 to 12 carbon atoms or a salt thereof [hereinafter referred to as component (a)]; and optionally a surfactant other than component (a) [hereinafter referred to as component (b)], wherein, in the case where component (b) is contained, (a) / (b), which is the mass ratio of the content of component (a) to the content of component (b), is 0.001 or more and 50 or less.

[0150] <46> (a) Use of a sulfosuccinic acid branched alkyl ester having a branched alkyl group having from 9 to 12 carbon atoms or a salt thereof as an enzyme reaction accelerator.

[0151] <47> A dishwasher detergent composition comprising: (a) a branched alkyl sulfosuccinate ester having a branched alkyl group having from 9 to 12 carbon atoms [hereinafter referred to as component (a)], and (d) an enzyme [hereinafter referred to as component (d)].

[0152] <48> The dishwasher detergent composition according to <47>, in which the branched alkyl group of component (a) has a main chain of 6 or 7 carbon atoms and one or more side chains, and the total number of carbon atoms in the side chains is 2 or more and 4 or less.

[0153] <49> The dishwasher detergent composition according to <47> or <48>, in which the branched alkyl group of the component (a) is one or more branched alkyl groups selected from a 2-propylheptyl group and a 3,5,5-trimethylhexyl group.

[0154] <50> The dishwasher detergent composition according to any one of <47> to <49>, wherein the component (a) is a branched alkyl sulfosuccinate ester represented by the following formula 1:

[0155]

[0156] [In the formula, R １ , R ２ are each a branched alkyl group having 9 to 12 carbon atoms, and A １ O.A. ２ Each O is an alkyleneoxy group having 2 to 4 carbon atoms, x1 and x2 are the average number of moles added and are each a number of 0 to 10, and M is a cation.

[0157] <51> In formula 1, R １ , R ２ The dishwasher detergent composition according to <50>, wherein the number of side chains of each of the above is 1 or more, and preferably 3 or less, more preferably 2 or less.

[0158] <52> In formula 1, R １ , R ２ The dishwasher detergent composition according to <50> or <51>, wherein the total number of carbon atoms in the branched chain hydrocarbon groups is independently 9 or 10, the number of carbon atoms in the main chain is independently 6 or 7, the number of carbon atoms constituting the side chains is independently 1 or more and 3 or less, and the number of side chains is independently 1.

[0159] <53> In formula 1, R １ , R ２ The dishwasher detergent composition according to any one of <50> to <52>, wherein the branched alkyl groups are the same or different and are selected from a 2-propylheptyl group and a 3,5,5-trimethylhexyl group.

[0160] <54> The dishwasher detergent composition according to any one of <50> to <53>, wherein, in formula 1, the average number of moles of x and y added is each independently 0.

[0161] <55> The dishwasher detergent composition according to any one of <50> to <54>, wherein, in formula 1, M is an inorganic cation.

[0162] <56> The dishwasher detergent composition according to any one of <47> to <55>, containing 0.01% by mass or more and 5% by mass or less of the component (a).

[0163] <57> The dishwasher detergent composition according to any one of <47> to <56>, wherein the component (d) is one or more enzymes selected from amylase, protease, and lipase.

[0164] <58> The dishwasher detergent composition according to any one of <47> to <57>, wherein the component (d) is preferably two or more enzymes selected from amylase, protease, and lipase, and is two or more enzymes including at least amylase and protease.

[0165] <59> The dishwasher detergent composition according to any one of <47> to <58>, containing 0.05% by mass or more, preferably 0.1% by mass or more, and 3% by mass or less, preferably 2% by mass or less, and more preferably 1% by mass or less of the component (a).

[0166] <60> The dishwasher detergent composition according to any one of <47> to <59>, containing the component (d) in an amount, as an enzyme protein, of preferably 0.001% by mass or more, more preferably 0.005% by mass or more, even more preferably 0.01% by mass or more, and preferably 15% by mass or less, more preferably 9% by mass or less, even more preferably 5% by mass or less.

[0167] <61> The dishwasher detergent composition according to any one of <47> to <60>, wherein the mass ratio (a) / (d) of the content of the component (a) to the content of the component (d) is preferably 0.1 or more, more preferably 1 or more, even more preferably 10 or more, and is preferably 50 or less, more preferably 40 or less, even more preferably 30 or less.

[0168] <62> The dishwasher detergent composition according to any one of <47> to <61>, further comprising (b4) a nonionic surfactant [hereinafter referred to as component (b4)].

[0169] <63> The dishwasher detergent composition according to <62>, wherein the component (b4) is one or more nonionic surfactants selected from alkyl glycosides, alkyl glyceryl ethers, and polyoxyalkylene alkyl ethers.

[0170] <64> The dishwasher detergent composition according to <62> or <63>, wherein the component (b4) is preferably a polyoxyalkylene alkyl ether in which the alkyl group is derived from a secondary alcohol and an average number of oxyethylene groups added is 1 to 10, in moles, added to the secondary alcohol.

[0171] <65> The dishwasher detergent composition according to any one of <63> to <64>, containing the component (b4) in an amount of preferably 0.05% by mass or more, more preferably 0.1% by mass or more, even more preferably 0.3% by mass or more, and preferably 5% by mass or less, more preferably 3% by mass or less, and even more preferably 1.0% by mass or less.

[0172] <66> The dishwasher detergent composition according to any one of <47> to <65>, further comprising one or more foam inhibitors selected from silicone oil, polypropylene glycol, and hydrophobic silica particles.

[0173] <67> The dishwasher detergent composition according to <66>, in which the weight-average molecular weight of the polypropylene glycol as the foam suppressor is preferably 1,000 or more, more preferably 2,000 or more, even more preferably 3,000 or more, and preferably 10,000 or less, more preferably 8,000 or less, even more preferably 5,000 or less.

[0174] <68> The dishwasher detergent composition according to any one of <47> to <67>, wherein the content of component (a) in all surfactants contained in the dishwasher detergent composition is 5% by mass or more, further 8% by mass or more, even 10% by mass or more, and is 100% by mass or less, further 50% by mass or less, even 30% by mass or less.

[0175] <69> The dishwasher detergent composition according to any one of <47> to <68>, wherein the dishwasher detergent composition of the present invention optionally contains an anionic surfactant other than component (a), and the content of component (a) in all anionic surfactants is preferably 50% by mass or more, more preferably 90% by mass or more and 100% by mass or less.

[0176] <70> The dishwasher detergent composition according to any one of <47> to <69>, further comprising one or more chelating agents selected from citric acid, glutamic acid diacetate, and salts thereof.

[0177] <71> The dishwasher detergent composition according to any one of <47> to <70>, which is a liquid and has a pH at 25°C of preferably 5 or more, more preferably 6 or more, even more preferably 7 or more, and preferably 11 or less, more preferably 10 or less, even more preferably 8 or less.

[0178] <72> The dishwasher detergent composition according to any one of <47> to <71>, wherein the dishwasher detergent composition is a liquid, and the viscosity of the composition at 25°C is preferably 10 mPa s or more, more preferably 50 mPa s or more, even more preferably 100 mPa s or more, and preferably 5,000 mPa s or less, more preferably 2,500 mPa s or less.

[0179] <73> The dishwasher detergent composition according to any one of <47> to <72>, wherein the dishwasher detergent composition is in the form of a powder and contains an alkaline agent, and the alkaline agent is one or more selected from sodium carbonate, potassium carbonate, sodium hydrogencarbonate, ethanolamine, sodium silicate, and potassium silicate.

[0180] <74> The dishwasher detergent composition according to any one of <47> to <73>, wherein the concentration (dilution concentration) of the dishwasher detergent composition during use is preferably at least 0.01% by mass, more preferably at least 0.05% by mass, even more preferably at least 0.1% by mass, and is preferably at most 2% by mass, more preferably at most 1% by mass, even more preferably at most 0.5% by mass.

[0181] <75> The dishwasher detergent composition according to any one of <47> to <74>, wherein the concentration of component (a) during use of the dishwasher detergent composition is preferably 1 ppm or more, more preferably 2 ppm or more, even more preferably 5 ppm or more, and is preferably 100 ppm or less, more preferably 50 ppm or less, even more preferably 30 ppm or less.

[0182] <76> The dishwasher detergent composition according to any one of <47> to <75>, wherein the concentration of component (d) during use of the dishwasher detergent composition is preferably 0.001 ppm or more, more preferably 0.005 ppm or more, even more preferably 0.01 ppm or more, and is preferably 5 ppm or less, more preferably 2 ppm or less, even more preferably 1 ppm or less.

[0183] <77> The dishwasher detergent composition according to any one of <47> to <76>, wherein a mixture of 0.2% by mass of the dishwasher detergent composition and water has a pH at 25°C of preferably 5 or more, more preferably 6 or more, even more preferably 6.5 or more, and preferably 12 or less, more preferably 11.5 or less, even more preferably 11 or less.

[0184] <78> A method for washing tableware, comprising washing tableware in a dishwasher using the dishwasher detergent composition according to any one of <47> to <77>.

[0185] <79> The method for washing tableware according to <78>, wherein tableware is washed in a dishwasher using a cleaning liquid obtained by diluting the detergent composition with water.

[0186] <80> The method for washing tableware according to <78> or <79>, wherein the washing temperature of the washing liquid is preferably 30°C or higher, more preferably 40°C or higher, and preferably 80°C or lower, more preferably 70°C or lower.

[0187] <81> Use of the composition according to any one of <47> to <77> as a detergent for dishwashers.

[0188] Examples <Example 1a and Comparative Example 1a> A triglyceride with a triolein / tristearin ratio of 8.5 / 1.5 (by mass) was dissolved in chloroform to prepare a model stain. A 75 mm (width) x 100 mm (length) x 1 mm (thickness) polypropylene test piece was uniformly coated with the model stain at a coating weight of 0.1 g, and the chloroform was evaporated and dried to prepare a stained piece. The aqueous medium shown in Table 1 was placed in a beaker, and the stained piece was immersed so that the entire area coated with the model stain was in contact with the aqueous medium. The temperature of the aqueous medium was 25°C. After immersion for 10 minutes, hydrochloric acid was added to inactivate the enzyme. The stained piece was removed and rinsed with running distilled water for 15 seconds. All of the water used during rinsing was recovered. After rinsing, the remaining stain on the stained piece was washed away with 50 ml of chloroform, and the resulting mixture was placed in a separatory funnel together with the water recovered during rinsing. After shaking several times, the lower chloroform layer was recovered. Thereafter, 25 ml of chloroform was added again, and after shaking several times again, the chloroform layer was recovered. This operation was repeated three times, after which the recovered chloroform was transferred to a recovery flask, and the chloroform was evaporated using an evaporator. 10 ml of methanol was then added to the recovery flask to dissolve the extract, and the amount of triglyceride (total amount of triolein and tristearin) after immersion was quantified by LC / MS. The triglyceride decomposition rate (I) was calculated from the triglyceride amount of the entire immersion blend using the following formula. The results are shown in Table 1. A higher triglyceride decomposition rate (I) indicates a more accelerated enzymatic reaction. Triglyceride decomposition rate (I) (%) = (total amount of triglycerides before immersion - total amount of triglycerides after immersion) / total amount of triglycerides before immersion × 100

[0189]

[0190] The components in the table are as follows: The mass % of each component in the table is based on the amount of the active ingredient (the same applies hereinafter). Component (a') is a comparative component to component (a). a-1: Sodium bis-(2-propylheptyl) sulfosuccinate a'-1: Sodium bis-(2-ethylhexyl) sulfosuccinate LAS: Sodium dodecylbenzenesulfonate, manufactured by FUJIFILM Wako Pure Chemical Industries, Ltd. AS: Sodium dodecyl sulfate, manufactured by FUJIFILM Wako Pure Chemical Industries, Ltd. FA: Sodium laurate, manufactured by FUJIFILM Wako Pure Chemical Industries, Ltd. AG: Alkyl polyglucoside, product name "Plantacare 2000UP", manufactured by BASF SE, alkyl group having 8 to 16 carbon atoms, glucose condensation degree 1 to 2 Betaine: Lauryl hydroxysulfobetaine, Amphitol 20HD, manufactured by Kao Corporation Amine oxide (1): Lauryl dimethylamine oxide, Amphitol 20N, manufactured by Kao Corporation Amine oxide (2): Octyl dimethylamine oxide, Amphitol 08N, manufactured by Kao Corporation Lipase: product name "Lipex Evity 100L", manufactured by Novozymes, molecular weight approximately 30,000

[0191] Example 2a The aqueous medium shown in Table 2 was filled into a pressure-accumulating foam discharge trigger-type spray container (Cucute Clear Foam Spray, manufactured by Kao Corporation) and sprayed five times onto soiled pieces prepared in the same manner as in Example 1a. The aqueous medium was discharged in foam form. The total amount of aqueous medium discharged was approximately 3 g. The components in Table 2 are the same as those used in Example 1a. After contacting the discharged foam with the soiled pieces for 1 minute, the pieces were rinsed with running distilled water for 15 seconds. The ambient temperature at this time was 25°C. All of the water used during rinsing was recovered. After rinsing, the remaining dirt on the plate was washed away with 50 ml of chloroform, and the mixture was placed in a separatory funnel together with the water recovered during rinsing. After shaking several times, the lower chloroform layer was recovered. 25 ml of chloroform was then added again, and the mixture was shaken several times again, after which the chloroform layer was recovered. This procedure was repeated three times, after which the recovered chloroform was transferred to a recovery flask and evaporated using an evaporator. Then, 10 ml of 2-propanol was added to the recovery flask to dissolve the extract. The amounts of each component, triolein (a), tristearin (b), diolein (c), distearin (d), monoolein (e), monostearin (f), oleic acid (g), stearic acid (h), and glycerin (i), were quantified by LC / MS based on their peak areas. The triglyceride decomposition rate (II) was calculated from the amounts of each component using the following formula. The results are shown in Table 2. A higher triglyceride decomposition rate (II) indicates a more accelerated enzymatic reaction. Triglyceride decomposition rate (II) (%) = {(c) + (d) + (e) + (f) + (g) + (h) + (i)} / {(a) + (b) + (c) + (d) + (e) + (f) + (g) + (h) + (i)} × 100

[0192]

[0193] In Table 2, the triglyceride decomposition rate (II) was high in the Examples using an aqueous medium containing component (b). This is thought to be because the quality of the foam formed from the aqueous medium differs depending on whether or not component (b) is present. That is, the foam produced from an aqueous medium containing component (b) has a finer texture and a longer lasting foam state than the foam produced from an aqueous medium not containing component (b). This is presumably because the opportunity for contact between the substrate and the enzyme, whose activity has been increased by component (a), is increased, resulting in a higher triglyceride decomposition rate (II).

[0194] Example 3a and Comparative Example 3a (1) Ingredients The ingredients used in Example 3a (Examples 3a-1 to 3a-10) and Comparative Example 3a (Comparative Examples 3a-1 to 3a-10) are as follows. Component (a') is a comparative ingredient to component (a). [Component (a)] a-1: sodium bis-(2-propylheptyl) sulfosuccinate [Component (a')] a'-1: sodium bis-(2-ethylhexyl) sulfosuccinate AS: sodium dodecyl sulfate, manufactured by FUJIFILM Wako Pure Chemical Industries, Ltd. [Enzyme] Protease: product name "Savinase 16L", manufactured by Novozymes [Other ingredients] Tris-HCl buffer solution (manufactured by Nippon Gene Co., Ltd.) Distilled water (manufactured by FUJIFILM Wako Pure Chemical Industries, Ltd.)

[0195] (1) Method for Evaluating Protease Activity: To 1 mL of each aqueous medium listed in Table 3, BSA (bovine serum albumin; Fujifilm Wako Pure Chemical Industries, Ltd.) was added as a protein substrate to a concentration of 50 ppm, and the mixture was stirred for 3 seconds using a vortex mixer and then incubated at 25°C. The aqueous media listed in Table 3 were prepared by mixing each component with the distilled water and adding Tris-HCl buffer (Nippon Gene Co., Ltd.) appropriately to adjust the pH to the value listed in the table. After 30 minutes, the reaction solution was mixed 1:1 with 2x Ez-Apply sample buffer (ATTO Corporation) containing 10 mM phenylmethylsulfonyl fluoride, stirred for 3 seconds using a vortex mixer, and then incubated at 100°C for 5 minutes. The electrophoresis gel used was e-Pagel e-T1020L (manufactured by ATTO Corporation), and the molecular weight marker was XL-Ladder Broad SP-2110 (manufactured by Aprosciences). Electrophoresis was performed at 40 mA for 80 minutes (WSE-1100PageRun-R (manufactured by ATTO Corporation)). One-step Ruby (manufactured by Aprosciences) was used to stain the gel. Fluorescent images of the gel were taken, and the band intensity in the 50-80 kDa region was measured from the acquired images using image analysis software (manufactured by Bio-Rad). The BSA degradation rate was calculated as an index of enzyme activity using the following formula, and the value of the BSA degradation rate was evaluated as follows. The results are shown in Table 3. In this evaluation, a BSA degradation rate of 30% or more is preferable, and a larger value is more preferable. BSA degradation rate (%) = 100 - (band intensity after degradation / undegraded band intensity) x 100

[0196]

[0197] In the table, the hardness of the aqueous medium is German hardness (° dH) measured by the following method. The hardness in the table was adjusted by dilution to the required hardness of the aqueous medium using a mother liquor of a 4000° DH hardness solution prepared by mixing 1 L of water, 83.9 g of calcium chloride dihydrate, and 29.0 g of magnesium chloride hexahydrate. In this specification, German hardness (° dH) refers to the concentration of calcium and magnesium in water expressed as CaCO ３German hardness refers to a concentration expressed as 1 mg / L (ppm) = approximately 0.056°dH (1°dH = 17.8 ppm). The calcium and magnesium concentrations for this German hardness are determined by chelate titration using disodium ethylenediaminetetraacetic acid. The specific method for measuring German hardness of aqueous media in this specification is described below. <Method for Measuring German Hardness of Aqueous Media> [Reagents] 0.01 mol / L EDTA·2Na solution: 0.01 mol / L aqueous solution of disodium ethylenediaminetetraacetate (titration solution, 0.01 M EDTA-Na2, manufactured by Sigma-Aldrich) Universal BT indicator (product name: Universal BT, manufactured by Dojindo Laboratories, Inc.) Ammonia buffer solution for hardness measurement (67.5 g of ammonium chloride was dissolved in 570 ml of 28 w / v% ammonia water, and the total volume was adjusted to 1000 ml with ion-exchanged water) [Hardness Measurement] (1) 20 ml of the sample aqueous medium was placed in a conical beaker using a volumetric pipette. (2) 2 ml of ammonia buffer solution for hardness measurement was added. (3) 0.5 ml of Universal BT indicator was added. It was confirmed that the solution after addition was reddish purple. (4) While shaking the conical beaker well, 0.01 mol / L EDTA·2Na solution was added dropwise from the burette, and the end point of the titration was the point when the aqueous medium sample turned blue. (5) The total hardness was calculated using the following formula: Hardness (°dH) = T × 0.01 × F × 56.0774 × 100 / A T: Titration volume (mL) of 0.01 mol / L EDTA·2Na solution A: Sample volume (20 mL, volume of aqueous medium sample) F: Factor of 0.01 mol / L EDTA·2Na solution

[0198] <Examples 1b, 2b and Comparative Examples 1b, 2b> In Examples 1b, 2b and Comparative Examples 1b, 2b, dishwasher detergent compositions containing the following components (a), (d), and (b4), etc., in the proportions shown in Tables 4 and 5, were prepared, and the cleaning performance for rice stains, protein cleaning performance (finishing quality), and cleaning performance for oil stains were evaluated, respectively.

[0199] <Component (a)> Sodium di-(2-propylheptyl) sulfosuccinate <Component (a')> Sodium di-(2-ethylhexyl) sulfosuccinate: Sanmorin OT-70 (manufactured by Sanyo Chemical Industries, Ltd.) <Component (d): Enzyme> Amylase: Duramyl 120T (manufactured by Novozymes) Protease: Savinase 18T-B (manufactured by Novozymes) <Component (b4)> Polyoxyethylene alkyl ether (average number of EO added: 5, alkyl group carbon atoms: 12-14), Softanol 50 (manufactured by Nippon Shokubai Co., Ltd.) <Other Components> Sodium polyoxypropylene alkyl ether sulfate (average number of PO added: 0.6, alkyl group carbon atoms: 8-12) Polypropylene glycol: polypropylene glycol (weight average molecular weight: 3000) (manufactured by Wako Pure Chemical Industries, Ltd.) Chelating agent: anhydrous citric acid (manufactured by Wako Pure Chemical Industries, Ltd.) Chelating agent: trisodium citrate, purified sodium citrate (manufactured by Fuso Chemical Co., Ltd.) Chelating agent: GLDA, tetrasodium L-glutamate diacetate (manufactured by Nouryon) Thickener: xanthan gum (manufactured by Tokyo Chemical Industry Co., Ltd.) Alkaline agent: sodium carbonate, soda ash (dense) (manufactured by Tokuyama Corporation) Glauber's salt: sodium sulfate decahydrate (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) Water

[0200] <Method for Evaluating Cleaning Performance> (1) Method for Evaluating Cleaning Performance for Rice Staining Contaminated bowls were prepared by applying 0.5 g of rice stain to a ceramic bowl and letting it dry overnight. Two contaminated bowls were placed inside an automatic dishwasher (Panasonic, model number: NP-45MS8S), with an identical, uncontaminated bowl placed inside to prevent direct contact with water. Six grams of the dishwasher detergent composition shown in the table were added, and the bowls were washed using the standard cycle. After washing, the remaining stains were visualized by staining with iodine, and the bowls were then rated according to the following criteria. The concentration of component (a) in the washing solution (formed by diluting the dishwasher detergent composition with water) used for the dishes was 10 ppm when 6 g of the composition was added. *Criteria for evaluating cleaning performance for rice staining: 3: The stain was completely removed. 2: Most of the stain was removed, but some remained. 1: Some stain remained, and the stain was mostly visible.

[0201] (2) Evaluation Method for Protein Cleaning Performance (Finish) (2-1) Method for Creating Protein Stain After preparing a soft-boiled egg (with the yolk soft-boiled and solidified) in a frying pan, only the egg liquid was collected using a strainer. (2-2) Evaluation Method Two clean glass cups were placed in an automatic dishwasher, and 10 g of the soft-boiled egg collected in (2-1) and 6 g of the dishwasher detergent composition shown in the table were added, followed by washing on the standard cycle. After washing, the dishes were scored based on the following criteria. The concentration of component (a) in the washing liquid applied to the dishes (the washing liquid formed by diluting the dishwasher detergent composition with water) was 10 ppm when 6 g of the composition was added. *Evaluation Criteria for Protein Cleaning Performance 3: Approximately 10 water spots and almost no dullness. 2: Approximately 20 to 50 water spots, or approximately 25% of the total surface is white and dull. 1: More than half of the surface is dull.

[0202] (3) Evaluation Method for Oily Stain Cleaning Performance Four polypropylene dishes were placed in an automatic dishwasher. Two of the dishes were contaminated with a composite soiling mixture of lard and rapeseed oil (1:1 by mass) at 3 g per dish, while the remaining two were uncontaminated. Six grams of the dishwasher detergent composition shown in the table was added, and the dishes were washed using the standard cycle. A total of three panelists touched the washed dishes and rated them according to the following criteria. Tables 4 and 5 show the average scores, rounded to the nearest whole number. The concentration of component (a) in the washing liquid (a washing liquid formed by diluting the dishwasher detergent composition with water) applied to the dishes was 10 ppm when 6 g of the composition was added. *Evaluation Criteria for Oily Stain Cleaning Performance 4: The stains were removed, and the dish felt a pleasant, squeaky feeling when touched. 3: The stains were removed, but the dish felt no squeaky feeling when touched. 2: Most of the dirt appears to have been removed, but rubbing with a finger leaves streaks. 1: Oil remains.

[0203] <Method for measuring pH> The dishwasher detergent composition of each Example and Comparative Example was diluted with water to a concentration of 0.2 mass %, and the pH of the cleaning liquid at 25° C. was measured by the glass electrode method. The results are shown in Tables 4 and 5.

[0204] <Method of measuring viscosity> The viscosity at 25°C of the dishwasher detergent compositions of the Examples and Comparative Examples in Table 5 was measured using a Brookfield viscometer (rotor No. 2, 6 rpm, measurement time 1 minute, liquid temperature 25°C). The results are shown in Table 5.

[0205]

[0206] Comparing Example 1b-1 with Comparative Examples 1b-1 and 1b-3 in Table 4, it is presumed that component (a) of the present invention further improves the decomposition reaction of the substrate (dirt) by component (d). Although Table 4 does not include an enzyme (lipase) that acts on oil, the high oil cleaning performance of Example 1b-1 and Comparative Example 1b-2 is thought to be due to the high washing temperature of the automatic dishwasher and the oil stain removal and dispersion effects based on the incorporation of component (a).

[0207]

[0208] In Table 5, comparing Example 2b-1 with Comparative Examples 2b-2 and 2b-5, it is inferred that the (a) component of the present invention further improves the decomposition reaction of the substrate (dirt) by the (d) component. In this evaluation, there was no difference in the cleaning performance of rice stains between Examples 2b-1 to 2b-3 and Comparative Example 2b-5. This is because the amylase used here was relatively easy to perform in a liquid composition, so no apparent difference occurred in this evaluation. For example, when evaluated under more severe cleaning conditions (such as when the cleaning solution is difficult to contact with the substrate during packed washing, or when targeting complex soiling containing rice and lipids), it is thought that differences may occur between these Examples and Comparative Examples. Furthermore, in Table 5, no enzyme (lipase) that acts on oil is blended. However, the high oil stain cleaning performance of Examples 2b-1 to 2b-3 and Comparative Example 2b-1 is thought to be due to the high washing temperature of the automatic dishwasher and the oil stain removal and dispersion effect based on the blending of component (a).

[0209] In Tables 4 and 5, the blending amount of component (d) is expressed as mass % in terms of enzyme protein.

Claims

1. A method for promoting an enzyme reaction, comprising reacting a substrate with an enzyme in the presence of (a) a branched alkyl sulfosuccinate ester having a branched alkyl group having from 9 to 12 carbon atoms or a salt thereof (hereinafter referred to as component (a)).

2. 2. The method for promoting an enzyme reaction according to claim 1, wherein the component (a) is a branched alkyl sulfosuccinate ester having a branched alkyl group of 9 or 10 carbon atoms or a salt thereof.

3. 2. The method for promoting an enzyme reaction according to claim 1, wherein an aqueous medium containing the component (a), the enzyme, and water is contacted with a substrate to react with the enzyme.

4. The method for promoting an enzyme reaction according to claim 3 , wherein the content of the component (a) in the aqueous medium is 0.01% by mass or more and 1.0% by mass or less.

5. The method for promoting an enzyme reaction according to claim 3, wherein the aqueous medium optionally contains a surfactant other than component (a) [hereinafter referred to as component (b)], and the content of component (b) in the aqueous medium is 3.0 mass% or less.

6. The method for promoting an enzyme reaction according to any one of claims 3 to 5, wherein the aqueous medium is contacted with the substrate in a foam state.

7. 2. The method for promoting an enzyme reaction according to claim 1, wherein the enzyme is one or more selected from the group consisting of protease, amylase, and lipase.

8. The method for promoting an enzyme reaction according to claim 1 , wherein the substrate is present in dirt attached to the hard surface of an article having a hard surface.

9. The method for promoting an enzyme reaction according to claim 1 , wherein the substrate is present in dirt attached to a fiber portion of an article containing fibers.

10. (a) An enzyme reaction promoter comprising, as an active ingredient, a branched alkyl sulfosuccinate ester or a salt thereof having a branched alkyl group having from 9 to 12 carbon atoms (hereinafter referred to as component (a)).

11. An enzyme reaction promoter composition comprising: (a) an enzyme reaction promoter containing, as an active ingredient, a branched alkyl sulfosuccinate ester having a branched alkyl group having from 9 to 12 carbon atoms or a salt thereof [hereinafter referred to as component (a)]; and, optionally, a surfactant other than component (a) [hereinafter referred to as component (b)]; and in the case where component (b) is contained, the mass ratio of the content of component (a) to the content of component (b), (a) / (b), is 0.001 or more and 50 or less.

12. A dishwasher detergent composition comprising: (a) a branched alkyl sulfosuccinate ester having a branched alkyl group containing from 9 to 12 carbon atoms, or a salt thereof [hereinafter referred to as component (a)]; and (d) an enzyme [hereinafter referred to as component (d)].

13. The dishwasher detergent composition according to claim 12, comprising 0.01% by mass or more and 5% by mass or less of the component (a).

14. The dishwasher detergent composition according to claim 12, wherein the component (d) is at least one selected from the group consisting of amylase, protease, and lipase.

15. 13. The dishwasher detergent composition according to claim 12, wherein the branched alkyl group of the component (a) is one or more branched alkyl groups selected from a 2-propylheptyl group and a 3,5,5-trimethylhexyl group.

16. The dishwasher detergent composition according to any one of claims 12 to 15, further comprising (b4) a nonionic surfactant.

17. The dishwasher detergent composition according to claim 12, wherein the mass ratio (a) / (d) of the content of the component (a) to the content of the component (d) is 0.1 or more and 50 or less.

18. A method for washing tableware, comprising washing tableware with a dishwasher using the detergent composition according to claim 12.

19. A method for washing tableware as described in claim 18, wherein tableware is washed in a dishwasher using a cleaning liquid obtained by diluting the detergent composition with water, and the temperature of the cleaning liquid is 30°C or higher and 80°C or lower.