Method for inducing differentiation into neural cells

Abstract

The present invention provides a method for inducing differentiation into nerve cells, comprising the steps of: a) culturing mesenchymal stem cells for 1 to 10 days in a stem cell culture medium comprising 60.0 to 90.0 vol% basal medium and 10.0 to 40.0 vol% physiological saline, to which 1.0 to 100.0 ng / mL EGF, 0.2 to 20.0 ng / mL FGF-2, 0.2 to 20.0 ng / mL PDGF, and 0.5 to 8.0 mM magnesium ascorbyl phosphate have been further added; b) thereafter, precipitating protein components of the culture solution obtained by the above culture with an organic solvent; and c) isolating and dissolving the precipitated portion in physiological saline or a basal medium, thereby culturing mesenchymal stem cells seeded at 3 x 104 to 2 x 105 cells / mL for 8 hours to 2 days at 4 to 10°C using the medium containing a cell-produced protein (CPP) composition, thereby differentiating mesenchymal stem cells into nerve cells.