Composite quaternary ammonium salt disinfectant and method for preparing the same

Abstract

A composite quaternary ammonium salt disinfectant includes a double long-chain quaternary ammonium salt, two single long-chain quaternary ammonium salts, a synergist isothiazolinone, a nonionic surfactant fatty alcohol polyvinyl ether, and water, wherein the double long-chain quaternary ammonium salt is dialkyl dimethyl ammonium chloride with dialkyl being two independent alkyl groups with a chain length of C8-C18, and the two single-chain quaternary ammonium salts are alkyl dimethyl benzyl ammonium chloride with an alkyl chain length of C8-C18 and alkyl dimethyl ethyl benzyl ammonium chloride with an alkyl chain length of C8-C18, respectively. The composite quaternary ammonium salt disinfectant has a synergistic effect among its components, which greatly improves the capacity of resisting interferences by hard water and organics; and can kill not only bacterial propagules, lipophilic viruses (enveloped viruses, such as novel coronavirus) and fungi, but also hydrophilic viruses (non-enveloped viruses).

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] The present invention is a Continuation of International Application No. PCT / CN2021 / 140734, filed Dec. 23, 2021, and claims priority to Chinese patent application No. 202011538060.0, filed on Dec. 23, 2020, and entitled “Composite quaternary ammonium salt disinfectant and method for preparing the same”, which are hereby incorporated by reference herein in their entireties.TECHNICAL FIELD

[0002] The invention relates to the field of disinfectants, and in particular to a composite quaternary ammonium salt disinfectant and a method for preparing the same.BACKGROUND ART

[0003] In recent years, new and sudden enveloped viruses, such as influenza, SARS, Middle East respiratory syndrome, Hog cholera, Ebola, and novel coronavirus, have posed a serious threat to human life and property. In the absence of specific drugs and vaccines, regular disinfection in public places and strengthening personal daily protection have been proved to be one of the effective means to control the rapid spread of novel coronavirus so far.

[0004] According to the list of disinfectants against novel coronavirus issued by EPA, a quaternary ammonium salt disinfectant accounts for a large proportion, which is an important disinfectant to combat the epidemic. Quaternary ammonium salts are a class of cationic surfactants, and the common quaternary ammonium salts have the general structural formula as follows:

[0005] wherein, R1-R2 represent organic groups, including alkyl, aryl, etc., which combine with a nitrogen atom to form a cationic group serving as an effective bactericidal part, and X is an anion, such as halogen, sulfate, or other analogs. In quaternary ammonium salts serving as disinfectants, R1 is usually an alkyl group with a chain length of C8-C18, and R2 is usually an aryl (for example, benzyl, ethyl benzyl) or alkyl group with a chain length of C8-C18. Quaternary ammonium salts, in which only one of R1 and R2 is long alkyl chain, are called single long-chain quaternary ammonium salts, such as alkyl dimethyl benzyl ammonium chloride and alkyl dimethyl ethyl benzyl ammonium chloride; and quaternary ammonium salts with both R1 and R2 being long alkyl chains are called double long-chain quaternary ammonium salts.

[0006] The combination of a traditional double long-chain quaternary ammonium salt and a single long-chain quaternary ammonium salt shows poor killing effect on hydrophilic viruses, and the killing effect of quaternary ammonium salt disinfectants is easily interfered by pollutants, such as hard water and organic pollutants, with the speed of kill needing further improvement.SUMMARY OF THE INVENTIONObject of the Invention

[0007] One object of the invention is to provide a composite quaternary ammonium salt disinfectant and a method for preparing the same. The composite quaternary ammonium salt disinfectant has a synergistic effect among its components, which greatly improves the capacity of resisting interferences by hard water and organics, and can kill not only bacterial propagules, lipophilic viruses (enveloped viruses, such as novel coronavirus) and fungi, but also hydrophilic viruses (non-enveloped viruses). In addition, the composite quaternary ammonium salt disinfectant provided by the invention has a rapid speed of kill.Technical Solutions

[0008] In order to achieve the object of the invention, the technical solutions provided by the invention are as follows:

[0009] A composite quaternary ammonium salt disinfectant is provided, which is prepared from raw materials comprising: a double long-chain quaternary ammonium salt, two single long-chain quaternary ammonium salts, a synergist isothiazolinone, a nonionic surfactant fatty alcohol polyvinyl ether, and water;

[0010] the double long-chain quaternary ammonium salt is dialkyl dimethyl ammonium chloride, in which dialkyl is two independent alkyl groups with a chain length of C8-C18; and the two single-chain quaternary ammonium salts are alkyl dimethyl benzyl ammonium chloride with an alkyl chain length of C8-C18 and alkyl dimethyl ethyl benzyl ammonium chloride with an alkyl chain length of C8-C18, respectively.

[0011] In one possible embodiment of the composite quaternary ammonium salt disinfectant, the double long-chain quaternary ammonium salt is one or more selected from the group consisting of didecyl dimethyl ammonium chloride, dioctyl dimethyl ammonium chloride, octyl decyl dimethyl ammonium chloride, didodecyl dimethyl ammonium chloride, ditetradecyl dimethyl ammonium chloride, and dihexadecyl dimethyl ammonium chloride;

[0012] optionally, the double long-chain quaternary ammonium salt is one or more selected from the group consisting of didecyl dimethyl ammonium chloride, dioctyl dimethyl ammonium chloride, octyl decyl dimethyl ammonium chloride, and didodecyl dimethyl ammonium chloride; further optionally, the double long-chain quaternary ammonium salt is one or more selected from the group consisting of didecyl dimethyl ammonium chloride, dioctyl dimethyl ammonium chloride, and octyl decyl dimethyl ammonium chloride.

[0013] In one possible embodiment of the composite quaternary ammonium salt disinfectant, alkyl dimethyl benzyl ammonium chloride with an alkyl chain length of C8-C18 is alkyl dimethyl benzyl ammonium chloride with an alkyl chain length of C10-C16, optionally alkyl dimethyl benzyl ammonium chloride with an alkyl chain length of C12-C16, and further optionally dodecyl dimethyl benzyl ammonium chloride;

[0014] and / or, alkyl dimethyl ethyl benzyl ammonium chloride with an alkyl chain length of C8-C18 is alkyl dimethyl ethyl benzyl ammonium chloride with an alkyl chain length of C10-C16, optionally alkyl dimethyl ethyl benzyl ammonium chloride with an alkyl chain length of C12-C14. In one possible embodiment of the composite quaternary ammonium salt disinfectant, the mass ratio of the double long-chain quaternary ammonium salt to the two single long-chain quaternary ammonium salts is 1: 0.20-1.0: 0.20-1.0.

[0015] In one possible embodiment of the composite quaternary ammonium salt disinfectant, the isothiazolinone is one or more selected from the group consisting of Kathon, 2-methyl-4-isothiazolin-3-one, 2-octyl-4-isothiazolin-3-one, and 1,2-benzisothiazolin-3-one; and / or, the fatty alcohol polyvinyl ether is AEO9 or AEO10.

[0016] In one possible embodiment of the composite quaternary ammonium salt disinfectant, the composite quaternary ammonium salt disinfectant further comprises one or more synergists selected from urotropine and o-phthalaldehyde;

[0017] and / or, the composite quaternary ammonium salt disinfectant further comprises one or more nonionic surfactants selected from glycosides, poloxamer, and polyvinyl alcohol. In one possible embodiment of the composite quaternary ammonium salt disinfectant, the total concentration of the double long-chain quaternary ammonium salt and two single long-chain quaternary ammonium salts in the composite quaternary ammonium salt disinfectant ranges from 2.0 g / L to 200 g / L (w / v), optionally from 5.0 g / L to 150 g / L (w / v), and further optionally from 10 g / L to 100 g / L (w / v).

[0018] In one possible embodiment of the composite quaternary ammonium salt disinfectant, the content of the synergist ranges from 0.01% to 1.0% (w / w), optionally from 0.02% to 0.5% (w / w), and further optionally from 0.03% to 0.3% (w / w);

[0019] and / or, the content of the nonionic surfactant ranges from 1.0% to 20% (w / w), optionally from 1.5% to 15% (w / w), and further optionally from 2.0% to 10% (w / w).

[0020] In one possible embodiment of the composite quaternary ammonium salt disinfectant, the composite quaternary ammonium salt disinfectant further comprises a pH modifier and / or alcohols or analogues;

[0021] the pH modifier is two or more selected from the group consisting of EDTA-4Na, EDTA-2Na, EDTA, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate and disodium hydrogen phosphate; optionally, the pH modifier is two or more selected from the group consisting of EDTA-4Na, EDTA-2Na, EDTA, potassium dihydrogen phosphate and dipotassium hydrogen phosphate; further optionally, the pH modifier is selected from two or three of EDTA-4Na, EDTA-2Na and EDTA;

[0022] and the alcohols or analogues are one or more selected from the group consisting of ethanol, propanol, isopropanol, dipropylene glycol, dipropylene glycol propyl ether, dipropylene glycol butyl ether, ethylene glycol, 1,2-propylene glycol, 1,3-propylene glycol, 1,2-butylene glycol, 1,2-pentanediol, 1,2-hexanediol, 1,2-heptanediol, 1,2-octanediol, 1,2-nonanediol, and 1,2-decanediol; optionally, the alcohols or analogues include one or more of ethanol, dipropylene glycol, dipropylene glycol propyl ether, dipropylene glycol butyl ether, ethylene glycol, 1,2-butanediol, 1,2-pentanediol, 1,2-hexanediol, 1,2-heptanediol, 1,2-octanediol, 1,2-nonanediol, and 1,2-decanediol; further optionally, the alcohols or analogues include one or more of ethanol, dipropylene glycol, dipropylene glycol propyl ether, dipropylene glycol butyl ether, 1,2-hexanediol and 1,2-octanediol. In one possible embodiment of the composite quaternary ammonium salt disinfectant, the content of the alcohols or analogues ranges from 0.5% to 15% (w / w), optionally from 0.8% to 10% (w / w), and further optionally from 1.0% to 8% (w / w);

[0023] and / or, the content of the pH modifier ranges from 0.2% to 15% (w / w), optionally from 0.5% to 10% (w / w), and further optionally from 2% to 5% (w / w).

[0024] The invention also provides a method for preparing the composite quaternary ammonium salt disinfectant, which comprises the steps of dissolving each component in purified water and stirring; optionally, the method further comprises the step of dilution after stirring and dissolving.Beneficial Effects

[0025] (1) The composite quaternary ammonium salt disinfectant provided by the invention is composed of a double long-chain quaternary ammonium salt, two single long-chain quaternary ammonium salts, a synergist and a nonionic surfactant, showing a synergistic effect among the components. The composite quaternary ammonium salt disinfectant provided by the invention has a higher anti-interference capacity compared to the traditional combination of a double long-chain quaternary ammonium salt and a single long-chain quaternary ammonium salt, which greatly improves the capacity of resisting interference by hard water, and can be directly diluted with tap water for using within 72 hours after dilution without affecting the killing capability on microorganisms. The composite quaternary ammonium salt disinfectant provided by the invention also greatly improves the capacity of resisting interference by organics, and can still kill Pseudomonas aeruginosa at the action time of 2 min in the case of interference by 50% calf serum, showing an excellent capacity of resisting interference by organics.

[0026] At the same time, the composite quaternary ammonium salt disinfectant provided by the invention can kill not only bacterial propagules, lipophilic viruses (enveloped viruses) and fungi, but also hydrophilic viruses (non-enveloped viruses). In addition, the composite quaternary ammonium salt disinfectant provided by the invention is capable of killing novel coronavirus rapidly in a test. The results show that the composite quaternary ammonium salt disinfectant provided by the invention can also rapidly kill novel coronavirus (2019-nCoV) and coronavirus (GX-P2V) in 30 s, 1 min and 3 min, with the killing logarithmic value being greater than 4.

[0027] The composite quaternary ammonium salt disinfectant provided by the invention has a higher speed of kill than the traditional combination of a double long-chain and a single long-chain quaternary ammonium salts.

[0028] (2) The composite quaternary ammonium salt disinfectant provided by the invention has the following advantages: easy to use, non-toxic, non-irritative, non-corrosive, resistant to interference by hard water, etc., and can be used for disinfecting various surfaces, air, hands, skin, mucous membranes, etc., as well as water bodies, aquaculture industries and other fields, which is suitable for large-scale promotion. The method for preparing the composite quaternary ammonium salt disinfectant provided by the invention is the method for preparing a disinfectant stock solution, which, during use in practice, can be used only by diluting to a specified concentration in proportion. Therefore, the quaternary ammonium salt disinfectant prepared by diluting the stock solution should also fall within the scope of the invention.DETAILED DESCRIPTION OF THE INVENTION

[0029] In order to make the object, technical solutions and advantages of the invention clearer, the technical solutions in the embodiments of the invention will be described clearly and completely, obviously, the described embodiments are some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work are within the scope of the present invention. In addition, in order to better explain the present invention, a lot of specific details are given in the following embodiments. It will be understood by those skilled in the art that the present invention may be practiced without certain specific details. In some embodiments, materials, elements, methods, means, etc., well known to those skilled in the art, are not described in detail so as to highlight the spirit of the present invention.

[0030] Throughout the specification and claims, the term “comprising” or variations thereof, such as “including” or “containing” and the like, will be understood to include the stated elements or components and not to exclude other elements or other components, unless expressly indicated otherwise.

[0031] In the following examples, all the raw materials used are common commercially available raw materials, as shown in Table 1:

[0032] TABLE 1List of raw materialsNameCASContentSupplierDidecyl dimethyl7173-51-5≥70%Shanghai Yuyu ammonium chlorideNew MaterialTechnology Co., Ltd.Octyl decyl dimethyl32426-11-2≥70%Shanghai Yuyu ammonium chlorideNew MaterialTechnology Co., Ltd.Dioctyl dimethyl5538-94-3≥70%Shanghai Yuyu ammonium chlorideNew MaterialTechnology Co., Ltd.Dodecyl dimethyl 139-07-1≥50%Shanghai Yuyu benzyl ammonium New MaterialchlorideTechnology Co., Ltd.C12-C16 alkyl 68424-85-1≥50%Shanghai Yuyu dimethyl benzylNew Materialammonium chlorideTechnology Co., Ltd.C12-C14 alkyl 85409-23-0≥50%Shanghai Yuyu dimethyl ethyl benzyl New Materialammonium chlorideTechnology Co., Ltd.Kathon (a mixture of—≥14%Dalian Baiao Chemical CMIT and MIT, Co., Ltd.CMIT:MIT = 3:1)MIT (2-methyl-4-2682-20-4≥50%Dalian Baiao Chemical isothiazolin-3 -one)Co., Ltd.OIT (2-octyl-4-26530-20-1≥45%Dalian Baiao Chemical isothiazolin-3-one)Co., Ltd.BIT (1,2-benziso-2634-33-5≥20%Dalian Baiao Chemical thiazolin-3-one)Co., Ltd.Urotropine100-97-0≥98%China National PharmaceuticalGroup Co., Ltd.o-Phthalaldehyde643-79-8≥98%China National PharmaceuticalGroup Co., Ltd.AEO9 (fatty alcohol68439-50-9≥98%Shanghai Yuyu polyoxyethylene etherNew Material(C12-C14 alcohol))Technology Co., Ltd.Poloxamer 1889003-11-695.0%-Shanghai Yuyu 102.0%New MaterialTechnology Co., Ltd.EDTA-4Na13235-36-4≥99%China National PharmaceuticalGroup Co., Ltd.EDTA-2Na139-33-3_≥99%China National PharmaceuticalGroup Co., Ltd.EDTA60-00-4≥99%China National PharmaceuticalGroup Co., Ltd.Dipotassium hydrogen7758-11-4≥99%China National phosphatePharmaceuticalGroup Co., Ltd.Potassium dihydrogen7778-77-0≥99%China National phosphatePharmaceuticalGroup Co., Ltd.Ethanol64-17-5≥99%China National PharmaceuticalGroup Co., Ltd.Ethylene glycol107-21-1≥99%China National PharmaceuticalGroup Co., Ltd.Dipropylene glycol110-98-5≥98%China National PharmaceuticalGroup Co., Ltd.Dipropylene glycol 29911-28-2≥98%Shanghai Yuyu butyl etherNew MaterialTechnology Co., Ltd.1,2-Octanediol1117-86-8≥98%Shanghai Yuyu New MaterialTechnology Co., Ltd.Water7732-18-5PurifiedHomemadeWaterEXAMPLE 1

[0033] Didecyl dimethyl ammonium chloride (2.86 g), dodecyl dimethyl benzyl ammonium chloride (4.0 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (4.0 g), Kathon (0.5 g), AEO9 (4.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.8 g), and ethanol (1.0 g) were dissolved in purified water (80.44 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.EXAMPLE 2

[0034] Didecyl dimethyl ammonium chloride (2.86 g), dodecyl dimethyl benzyl ammonium chloride (4.0 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (4.0 g), Kathon (0.1 g), AEO9 (4.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.8 g), and ethanol (1.0 g) were dissolved in purified water (80.84 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.EXAMPLE 3

[0035] Didecyl dimethyl ammonium chloride (2.86 g), dodecyl dimethyl benzyl ammonium chloride (4.0 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (4.0 g), Kathon (0.5 g), urotropine (2.5 g), AEO9 (4.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.8 g), and octanediol (2.0 g) were dissolved in purified water (76.94 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.EXAMPLE 4

[0036] Didecyl dimethyl ammonium chloride (2.86 g), C12-C16 alkyl dimethyl benzyl ammonium chloride (4.0 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (4.0 g), MIT (0.25 g), urotropine (1.25 g), AEO9 (4.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.4 g), EDTA (0.4 g), octanediol (1.0 g), and ethanol (1.0 g) were dissolved in purified water (78.44 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.EXAMPLE 5

[0037] Didecyl dimethyl ammonium chloride (1.43 g), dioctyl dimethyl ammonium chloride (1.43 g), dodecyl dimethyl benzyl ammonium chloride (4.0 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (4.0 g), MIT (0.5 g), AEO9 (4.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.8 g), and ethanol (1.0 g) were dissolved in purified water (80.44 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.EXAMPLE 6

[0038] Didecyl dimethyl ammonium chloride (1.28 g), octyl decyl dimethyl ammonium chloride (2.56 g), dioctyl dimethyl ammonium chloride (1.28 g), C12-C16 alkyl dimethyl benzyl ammonium chloride (2.4 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (2.4 g), MIT (0.5 g), AEO9 (4.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.8 g), and dipropylene glycol (1.0 g) were dissolved in purified water (81.38 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.EXAMPLE 7

[0039] Didecyl dimethyl ammonium chloride (1.28 g), octyl decyl dimethyl ammonium chloride (2.56 g), dioctyl dimethyl ammonium chloride (1.28 g), dodecyl dimethyl benzyl ammonium chloride (2.4 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (2.4 g), Kathon (0.5 g), AEO9 (4.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.8 g), octanediol (2.0 g), and ethanol (10.0 g) were dissolved in purified water (70.38 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.EXAMPLE 8

[0040] Didecyl dimethyl ammonium chloride (2.86 g), dodecyl dimethyl benzyl ammonium chloride (4.0 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (4.0 g), o-phthalaldehyde (1.25 g), MIT (0.25 g), poloxamer 188 (2.0 g), AEO9 (2.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.8 g), dipropylene glycol (4.0 g), and octanediol (2.0 g) were dissolved in purified water (74.44 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.EXAMPLE 9

[0041] Didecyl dimethyl ammonium chloride (0.72 g), octyl decyl dimethyl ammonium chloride (1.44 g), dioctyl dimethyl ammonium chloride (0.72 g), C12-C16 alkyl dimethyl benzyl ammonium chloride (4.0 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (4.0 g), BIT (0.5 g), urotropine (0.5 g), AEO9 (4.0 g), dipotassium hydrogen phosphate (2.0 g), potassium dihydrogen phosphate (1.2 g), and ethylene glycol (5.0 g) were dissolved in purified water (75.92 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.EXAMPLE 10

[0042] Didecyl dimethyl ammonium chloride (1.43 g), C12-C16 alkyl dimethyl benzyl ammonium chloride (6.0 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (6.0 g), OIT (0.5 g), AEO9 (2.0 g), poloxamer 188 (2.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.8 g), 1,2-octanediol (1.0 g), and ethylene glycol (4.0 g) were dissolved in purified water (73.87 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.COMPARATIVE EXAMPLE 1

[0043] Didecyl dimethyl ammonium chloride (2.86 g), dodecyl dimethyl benzyl ammonium chloride (4.0 g), C12-C14 alkyl dimethyl ethyl benzyl ammonium chloride (4.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.8 g), and ethanol (1.0 g) were dissolved in purified water (84.94 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.COMPARATIVE EXAMPLE 2

[0044] Didecyl dimethyl ammonium chloride (2.86 g), dodecyl dimethyl benzyl ammonium chloride (8.0 g), Kathon (0.5 g), AEO9 (4.0 g), EDTA-4Na (2.4 g), EDTA-2Na (0.8 g), and ethanol (1.0 g) were dissolved in purified water (80.44 g) with stirring, to prepare a stock solution of composite quaternary ammonium salt disinfectant, which was diluted by 40 times for use in various tests in the application.COMPARATIVE EXAMPLE 3

[0045] Commercially available Dr. Xiao's compound double long-chain quaternary ammonium salt disinfectant is used, which consists of didecyl dimethyl ammonium chloride and octyl decyl dimethyl ammonium chloride, having the content of active ingredients of 1.7-2.0 g / L.Test 1: Test for Physico-Chemical Indicators

[0046] The physico-chemical indicators of the samples of Examples 1-10 were tested respectively by using the samples of Comparative Example 1-3 as the controls. The test items and test methods are shown in Table 2.

[0047] TABLE 2Test items and methodsItemsMethodsSensory stateVisual inspection under indirect sunlight.Content of activeAccording to Appendix A 2.1 of GB 26369.ingredientspH valueAccording to 2.2.1.4 of Technical Standard forDisinfection (2002 edition), pH 4-10.Corrosion to metalsAccording to 2.2.4 of Technical Standard forDisinfection (2002 edition).

[0048] The results are shown in Table 3.

[0049] TABLE 3Test data for physico-chemical propertiesPhysico-chemical indicatorsContent ofactivepHSensory stateingredientsvalueCorrosion to metalsComparativeColorless transparent1.32 g / L9.62Noncorrosive to stainless steel,example 1liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.ComparativeColorless transparent1.28 g / L9.63Noncorrosive to stainless steel,example 2liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.ComparativeColorless transparent1.99 g / L5.81—example 3liquid, odorless, no visibleinsoluble matter.Example 1Light yellow transparent1.21 g / L8.96Noncorrosive to stainless steel,liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.Example 2Colorless transparent1.18 g / L9.02Noncorrosive to stainless steel,liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.Example 3Light yellow transparent1.23 g / L8.85Noncorrosive to stainless steel,liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.Example 4Colorless transparent1.22 g / L9.01Noncorrosive to stainless steel,liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.Example 5Colorless transparent1.19 g / L8.68Noncorrosive to stainless steel,liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.Example 6Colorless transparent1.21 g / L8.93Noncorrosive to stainless steel,liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.Example 7Light yellow transparent1.25 g / L8.86Noncorrosive to stainless steel,liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.Example 8Colorless transparent1.27g / L9.05Noncorrosive to stainless steel,liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.Example 9Light yellow transparent1.23 g / L8.88Noncorrosive to stainless steel,liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.Example 10Colorless transparent1.19 g / L8.99Noncorrosive to stainless steel,liquid, odorless, no visibleand mildly corrosive to carboninsoluble matter.steel, copper sheet andaluminum sheet.Test 2: Test for Killing Microorganisms

[0050] A test for the effect on air disinfection was conducted using the sample of Example 1 as a testing disinfectant according to the method of 2.1.3 in Technical Standard for Disinfection (2002 edition), and the test results are shown in Table 4.

[0051] TABLE 4Test data for killing microorganisms (Air disinfection)Simulated field testField testspraying evenly in the test spraying evenly in an empty office chamber at a disinfectant in a volume of about 50 m3 at aDisinfectantdosage of 5 mL / m3,disinfectant dosage of 5 mL / m3, usedthen standing for treatmentthen standing for treatmentExample 1In three parallel tests, the killing In three parallel tests, the killingrates on Staphylococcus albus,rates on natural bacteria in the airStaphylococcus aureus andwere all ≥ 90% after 5 min.Escherichia coli at 5 × 105 CFU / m3were all ≥ 99.90% after 5 min.Test 3: Test for Inactivating Novel Coronavirus (2019-nCoV)

[0052] This test was commissioned to Analytical and Testing Center of Capital Regions Co., Ltd. to carry out a test for inactivating novel coronavirus (2019-nCoV) using the composite quaternary ammonium salt disinfectant provided by the inventive embodiment according to the method of 2.1.1.10.7 in Technical Standard for Disinfection (2002 edition), and the data are shown in Table 5.

[0053] TABLE 5Test Data for inactivating novel coronavirusThe testing strain was a novel coronavirus (2019-nCoV)Example 1 The The antiviral activity rate against the novel active ingredient coronavirus (2019-nCoV) at the action time was 1.21 g / L.of 30 s, 1 min and 3 min were all > 99.99%.Test 4: Test for Killing Enveloped Virus

[0054] This test was commissioned to College of Life Science and Technology, Beijing University of Chemical Technology, to carry out the test for inactivating coronavirus (GX-P2V), which was a novel coronavirus isolated from pangolins, and had 92.2% homology with the spike protein of novel coronavirus (2019-nCoV) and 90% identity in term of the amino acid sequence, thus being suitable as a substitution model for screening anti-novel coronavirus drugs (see Lam, T. T., Jia, N., Zhang, Y. et al. Identifying SARS-CoV-2-related coronaviruses in Malayan Pangolin S. Nature (2020) for the virus and properties thereof. https: / / doi.org / 10.1038 / s41586-020-2169-0). The inactivation test was conducted using the sample of Example 1 as a testing disinfectant and a coronavirus (GX-P2V) as a testing virus strain according to the method of 2.1.1.10.7 in Technical Standard for Disinfection (2002 edition), and the test results are shown in Table 6.

[0055] TABLE 6Test data for inactivating coronavirusMicroorganism killing indicators (coronavirus (GX-P2V))Example 1 The The inactivation efficiency on the active ingredient coronavirus at the action time of 30 s, was 1.21 g / L.1 min and 3 min were all > 99.99%.Test 5: Test for Inactivating Non-Enveloped Virus

[0056] The inactivation test was conducted using the sample of Example 1 as a testing disinfectant and poliovirus as a testing virus strain according to the method of 2.1.1.10.7 in Technical Standard for Disinfection (2002 edition), and the test results are shown in Table 7.Test 7 Test for Inactivating Non-Enveloped Viruses

[0057] Testing virus strain (poliovirus)Example 1 The The inactivation efficiency on the active ingredient poliovirus at the action time of 2.5 min, was 1.21 g / L.5 min and 15 min were all > 99.99%.Test 6: Organic Interference Test

[0058] The test was conducted by using the samples of Example 1 and Comparative Examples 1 and 2 as disinfectants, according to the method of 2.1.10.2.3 in Technical Standard for Disinfection (2002 edition), in which 0, 25% and 50% calf serum were added, respectively, as organic interferents, and Pseudomonas aeruginosa was used as a testing strain, to study the effect of organics on the sample disinfection, and the results are shown in Table 8.Test 8 Organic Interference Test

[0059] Action timeContent of Killing logarithmic valuecalf serum30 s1 min3 min5 minSample of0≥5.00———Example 125%≥5.00———50%3.524.68≥5.00≥5.00Action timeContent of Killing logarithmic valuecalf serum30 s1 min3 min5 minSample of0≥5.00———Example 825%≥5.00———50%2.284.35≥5.00≥5.00Action timeContent of Killing logarithmic valuecalf serum5 min10 min15 min20 minSample of0≥5.00———Comparative25%1.822.283.54≥5example 150%1.562.462.833.66Action timeContent of Killing logarithmic valuecalf serum5 min10 min15 min20 minSample of0≥5.00———Comparative25%1.762.583.964.38example 250%1.452.322.833.66

[0060] Under the organic interference, the composite quaternary ammonium salt disinfectants provided by Example 1 and Example 8 of the invention can tolerate the interference of 25% and 50% calf serum, and kill the test strains in a short time, while those provided by the Comparative examples 1 and 2 can not tolerate the interference of 50% calf serum. The composite quaternary ammonium salt disinfectant provided by the example of the invention can be applied more conveniently and has a more extensive application field as compared with Comparative examples 1 and 2.Test 7: Hard Water Interference Test

[0061] The test was conducted according to the method of 2.1.1.2 in Technical Standard for Disinfection (2002 edition), using diluents of the disinfectant stock solutions provided by Example 1, Example 8, Comparative Example 1 and Comparative Example 2 of the application as testing disinfectants, respectively, and Pseudomonas aeruginosa as a testing strain, wherein each of the disinfectant stock solution was diluted by 40 times with common tap water, followed by standing for 72 hours, to obtain the diluent, as shown in Table 9.

[0062] TABLE 9Hard water interference testTestingdisinfectantMicroorganism killing indicatorsExample 1Action time: Action time: Action time: 30 s,1 min,1.5 min,Killing Killing Killing logarithmiclogarithmiclogarithmicvalue ≥ 5value ≥ 5value ≥ 5Example 8Action time: Action time: Action time: 30 s,1 min,1.5 min,Killing Killing Killing logarithmiclogarithmiclogarithmicvalue ≥ 5value ≥ 5value ≥ 5Com-Action time: Action time: Action time: parative5 min,10 min,15 min,example 1Killing Killing Killing logarithmiclogarithmiclogarithmicvalue is 2.35.value is 3.56.value is 4.05.Com-Action time: Action time: Action time: parative5 min,10 min,15 min,example 2Killing Killing Killing logarithmiclogarithmiclogarithmicvalue is 1.85.value is 2.78.value is 3.12.Test 8: Test for the Speed of Killing Microorganisms

[0063] The tests for killing microorganisms were conducted using the samples of Example 1-10 as testing disinfectants, respectively, and the samples of Comparative Example 1-3 as controls, as well as Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans as test strains. All the above tests were carried out according to the method of 2.1.1.2 in Technical Standard for Disinfection (2002 edition), and the test results are shown in Table 10.

[0064] TABLE 10Test data for killing microorganismsMicroorganism killing indicatorsStaphylococcus aureusPseudomonas aeruginosaCandida albicansTime taken when theTime taken when theTime taken when theTestingaverage killingaverage killingaverage killingdisinfectantlogarithmic value ≥ 5.00logarithmic value ≥ 5.00logarithmic value ≥ 5.00Comparative  5 min  5 min  5 minexample 1Comparative  5 min  5 min  5 minexample 2Comparative  5 min  5 min  5 minexample 3Example 10.5 min0.5 min0.5 minExample 20.5 min0.5 min0.5 minExample 30.5 min0.5 min0.5 minExample 40.5 min0.5 min0.5 minExample 50.5 min0.5 min0.5 minExample 60.5 min0.5 min0.5 minExample 70.5 min0.5 min0.5 minExample 80.5 min0.5 min0.5 minExample 90.5 min0.5 min0.5 minExample 100.5 min0.5 min0.5 min

[0065] It can be seen from the table above that, when the action time was shortened from 5 min to 0.5 min, the composite quaternary ammonium salt disinfectants obtained in the inventive examples achieved the same bactericidal effect, as compared with the disinfectants obtained in the Comparative examples.Test 9: Toxicological Indicators

[0066] A test for toxicological indicators was conducted using the sample of Example 1 as a testing disinfectant according to the method of 2.3.1, 2.3.2, 2.3.3, 2.3.4 and 2.3.8 in Technical Standard for Disinfection (2002 edition), and the test results are shown in Table 11.

[0067] TABLE 11Toxicological indicatorsItemsTest resultsAcute oral toxicityThe acute oral toxicity LD50 for mice was greater than 5000mg / Kg, which was actually nontoxic according to the acute toxicity evaluation rules of Technical Standard for Disinfection (2002 edition).Acute inhalation The acute inhalation toxicity Lc50 for mice was greater than toxicity10000 mg / m3, which was actually nontoxic according to the acute toxicity evaluation rules of Technical Standard for Disinfection (2002 edition).Mutagenicity testNo chromosome damage occurred in mice.Multiple skin irritationAfter continuous application for 14 days, no swelling and tests (repeated use)edema occurred in rabbits, which was considered as non-irritative according to 2.3.3 Grading standard of skin irritation intensity of Technical Standard for Disinfection (2002 edition).Multiple acute eyeDuring the observation period, no conjunctival congestion,irritation tests conjunctival edema, iris injury and corneal opacity occurred (repeated use)in rabbits, which was considered as non-irritative according to 2.3.4 Grading standard of eye irritation of Technical Standard for Disinfection (2002 edition).

[0068] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit it; although the present invention has been described in detail with reference to the foregoing examples, it will be understood by one of ordinary skill in the art that the technical solutions described in the foregoing examples can still be modified or some technical features can be equivalently substituted; however, these modifications or substitutions do not make the essence of the corresponding technical solutions depart from the spirit and scope of the technical solutions of various examples of the present invention.INDUSTRIAL APPLICABILITY

[0069] The composite quaternary ammonium salt disinfectant provided by the invention has a synergistic effect among its components, which greatly improves the capacity of resisting interferences by hard water and organics; and can kill not only bacterial propagules, lipophilic viruses (enveloped viruses, such as novel coronavirus) and fungi, but also hydrophilic viruses (non-enveloped viruses). In addition, the composite quaternary ammonium salt disinfectant provided by the invention has the following advantages: having a rapid speed of kill, easy to use, non-toxic, non-irritative, and non-corrosive, and can be used for disinfecting various surfaces, air, hands, skin, mucous membranes, etc., as well as water bodies, aquaculture industries and other fields, which is suitable for large-scale promotion and shows a good market prospect.

Claims

1. A composite quaternary ammonium salt disinfectant, wherein the raw materials thereof comprise: a double long-chain quaternary ammonium salt, two single long-chain quaternary ammonium salts, a synergist isothiazolinone, a nonionic surfactant fatty alcohol polyoxyethylene ether, and water;the double long-chain quaternary ammonium salt is one or more selected from the group consisting of didecyl dimethyl ammonium chloride, dioctyl dimethyl ammonium chloride, and octyl decyl dimethyl ammonium chloride;the two single long-chain quaternary ammonium salts are alkyl dimethyl benzyl ammonium chloride with an alkyl chain length of C12-C16 or dodecyl dimethyl benzyl ammonium chloride; and alkyl dimethyl (ethylbenzyl) ammonium chloride with an alkyl chain length of C12-C14, respectively;the synergist isothiazolinone is one or more selected from the group consisting of 2-methyl-4-isothiazolin-3-one, 2-octyl-4-isothiazolin-3-one, 1,2-benzisothiazolin-3-one, and a mixture of 2-methyl-5-chloro-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one in a mass ratio of 3:1;the fatty alcohol polyoxyethylene ether is C12-C14 fatty alcohol polyoxyethylene (9) ether;the total concentration of the double long-chain quaternary ammonium salt and the two single long-chain quaternary ammonium salts in the composite quaternary ammonium salt disinfectant ranges from 2.0 g / L to 200 g / L (w / v);the mass ratio of the double long-chain quaternary ammonium salt to the two single long-chain quaternary ammonium salts is 1:0.20-1.0:0.20-1.0;the content of the synergist ranges from 0.01% to 1.0% (w / w); andthe content of the nonionic surfactant ranges from 1.0% to 20% (w / w).

2. The composite quaternary ammonium salt disinfectant according to claim 1, wherein the composite quaternary ammonium salt disinfectant further comprises one or more synergists selected from urotropine and o-phthalaldehyde;and / or,the composite quaternary ammonium salt disinfectant further comprises one or more nonionic surfactants selected from glycosides, poloxamer, and polyvinyl alcohol.

3. The composite quaternary ammonium salt disinfectant according to claim 1, wherein the total concentration of the double long-chain quaternary ammonium salt and the two single long-chain quaternary ammonium salts in the composite quaternary ammonium salt disinfectant ranges from 5.0 g / L to 150 g / L (w / v), and further optionally from 10 g / L to 100 g / L (w / v).

4. The composite quaternary ammonium salt disinfectant according to claim 1, whereinthe content of the synergist ranges from 0.02% to 0.5% (w / w), and further optionally from 0.03% to 0.3% (w / w);and / or,the content of the nonionic surfactant ranges from 1.5% to 15% (w / w), and further optionally from 2.0% to 10% (w / w).

5. The composite quaternary ammonium salt disinfectant according to claim 1, wherein the composite quaternary ammonium salt disinfectant further comprises a pH modifier and / or alcohols;the pH modifier is two or more selected from the group consisting of EDTA-4Na, EDTA-2Na, EDTA, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate and disodium hydrogen phosphate; optionally, the pH modifier is two or more selected from the group consisting of EDTA-4Na, EDTA-2Na, EDTA, potassium dihydrogen phosphate and dipotassium hydrogen phosphate; further optionally, the pH modifier is selected from two or three of EDTA-4Na, EDTA-2Na and EDTA;the alcohols are one or more selected from the group consisting of ethanol, propanol, isopropanol, dipropylene glycol, dipropylene glycol propyl ether, dipropylene glycol butyl ether, ethylene glycol, 1,2-propylene glycol, 1,3-propylene glycol, 1,2-butylene glycol, 1,2-pentanediol, 1,2-hexanediol, 1,2-heptanediol, 1,2-octanediol, 1,2-nonanediol, and 1,2-decanediol; optionally, the alcohols include one or more of ethanol, dipropylene glycol, dipropylene glycol propyl ether, dipropylene glycol butyl ether, ethylene glycol, 1,2-butanediol, 1,2-pentanediol, 1,2-hexanediol, 1,2-heptanediol, 1,2-octanediol, 1,2-nonanediol, and 1,2-decanediol; further optionally, the alcohols include one or more of ethanol, dipropylene glycol, dipropylene glycol propyl ether, dipropylene glycol butyl ether, 1,2-hexanediol and 1,2-octanediol.

6. The composite quaternary ammonium salt disinfectant according to claim 5, wherein, the content of the alcohols ranges from 0.5% to 15% (w / w), optionally from 0.8% to 10% (w / w), and further optionally from 1.0% to 8% (w / w);and / or,the content of the pH modifier ranges from 0.2% to 15% (w / w), optionally from 0.5% to 10% (w / w), and further optionally from 2% to 5% (w / w).

7. A method for preparing the composite quaternary ammonium salt disinfectant according to claim 1, wherein the method comprises the steps of dissolving each component in purified water and stirring; optionally, the method further comprises the step of dilution after stirring and dissolving.

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