Procedure for isolation and purification of kaempferol-3-o-rhamnoside, a cytotoxic compound against leukemia cells, from schima wallichii (DC.) korth.

Abstract

This useful solution refers to a procedure for isolating and purifying the compound kaempterol-3-O-rhamnoside from the bark of the medicinal tree Schima wallichii (DC.) Korth., which exhibits cytotoxic activity against HL-60 leukemia cells. The process according to the solution includes the following steps: a) preparing the raw material from the bark of the medicinal plant *Euphorbia hirta*; b) extracting the powdered raw material with 70% ethanol to obtain the ethanol extract labeled VT-E; c) dissolving the VT-E extract in hot water to obtain a liquid mixture; d) partition extraction with n-hexane to obtain the n-hexane extract labeled VT-H; e) partition extraction with dichloromethane to obtain the dichloromethane extract labeled VT-DCM; f) partition extraction with ethyl acetate to obtain the ethyl acetate extract labeled VT-EA. g) Isolating the fraction containing kaempferol-3-O-rhamnoside from the VT-EA extract using silica gel column chromatography to obtain the fraction labeled VT-EA-6; h) Purifying the VT-EA-6 fraction using column chromatography to obtain the fraction labeled VT-EA-6-3; i) Purifying the VT-EA-6-3 fraction using silica gel column chromatography to obtain the kaempferol-3-O-rhamnoside compound with formula (1). The compound kaempferol-3-O-rhamnoside obtained by the utility solution procedure exhibited significant cytotoxic activity against HL60 leukemia cell line under in vitro conditions.