Acne clearing composition for optimizing skin grease mass, preparation method therefor and use thereof

Abstract

An acne clearing composition for optimizing skin grease mass, comprising the following components in parts by mass: 0.1-5 parts of a mango leaf extract, 0.1-10 parts of a guaiacum officinale extract, 0.1-10 parts of N-acetylglucosamine, 0.1-10 parts of a yeast extract, and 0.01-5 parts of EUK-134. The components of the acne clearing composition can cooperate with each other, so that optimization of sebum components, maintenance of the stability of proportions of saturated fatty acids / unsaturated fatty acids of the sebum components and inhibition of sebum peroxidation are realized on the whole, thereby achieving the effects of mildly clearing acnes and repairing a sebum layer barrier, and the acne clearing composition has excellent antibacterial performance. Moreover, the acne clearing composition can be added into cosmetics as an active ingredient, so that the cosmetics are mild and non-irritating, and the preparation method for the acne clearing composition is simple and uses easily obtained raw materials, thereby facilitating actual production.

Description

An anti-acne composition for optimizing skin oil quality, and its preparation method and application Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to an anti-acne composition for optimizing skin oil quality, and a preparation method and application thereof. Background Art

[0002] Excessive sebum secretion, abnormal keratinization of the sebaceous glands of the hair follicles, Propionibacterium acnes and inflammation have always been considered to be the four major factors causing acne.

[0003] Currently, among the products on the market that claim to control oil, remove acne, and eliminate acne, on the one hand, most still single-mindedly and one-sidedly achieve the effect of suppressing acne from the perspective of comprehensively controlling sebum secretion, without considering the balance of the various components in sebum. Simply controlling oil may suppress both peroxidized and antioxidant sebum, which has no benefit in optimizing sebum structure. There is also a situation where high-quality sebum is overly suppressed while retaining sebum that is prone to acne and inflammation. This is especially true for people with oily and sensitive skin and those with recurrent acne. Simple oil control cannot effectively resolve acne inflammation and the formation of acne, resulting in acne being incurable. On the other hand, acid ingredients are also widely used in oil control and acne treatment products as powerful oil-control, exfoliating, and antibacterial ingredients. The high irritation caused by highly effective ingredients is not taken into account, causing the skin condition to deteriorate. At the same time, in order to enhance the long-term antibacterial properties of the components, certain antibacterial agents are added to the products, but antibacterial agents also have the disadvantage of being more irritating to a certain extent.

[0004] Summary of the Invention

[0005] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an acne-removing composition, a preparation method and application thereof, which can optimize sebum composition, maintain the stability of the ratio of saturated fatty acids to unsaturated fatty acids in sebum components, inhibit sebum peroxidation, gently remove acne, repair the sebum layer barrier and have excellent antibacterial properties.

[0006] To achieve the above objectives, in a first aspect of the present invention, the present invention provides an acne-removing composition for optimizing the quality of skin oil, the acne-removing composition comprising the following components in parts by weight: 0.1-5 parts of mango leaf extract, 0.1-10 parts of guaiac extract, 0.1-10 parts of acetyl glucosamine, 0.1-10 parts of yeast extract, and 0.01-5 parts of EUK-134.

[0007] The inventors' previous studies have found that imbalances in sebum secretion, an imbalance in the oxidation / antioxidation ratio of skin surface lipids, and changes in sebum composition are the main factors that induce acne inflammation, and may have a greater impact than excessive sebum secretion. Sebum is composed of triglycerides, free fatty acids, wax esters, squalene, cholesterol esters, and cholesterol. These lipids can be oxidized by various factors, such as porphyrins secreted by Propionibacterium acnes, singlet oxygen caused by ultraviolet rays, and free radical oxidation caused by oxygen in the atmosphere. Among the lipids that make up sebum, the oxidation of triglycerides to unsaturated fatty acids and the oxidation of squalene by singlet oxygen to produce peroxides—squalene monohydroperoxide (SQOOH)—are the most obvious changes after sebum oxidation. Peroxidized lipids can generate a large number of reactive oxygen free radicals by activating lipoxygenase, increasing the production of proinflammatory cytokines such as IL-6 and COX, inducing an inflammatory response, and are extremely cytotoxic, leading to reactive follicular hyperkeratosis and comedonal formation, thereby causing acne. The inventors further discovered that acne-prone skin secretes more sebum than normal skin, and the ratios of saturated and unsaturated fatty acids in the sebum of the two groups differ significantly. The levels of triglycerides and squalene in the sebum of people with acne are approximately twice that of people with normal skin, and the level of lipid peroxides is also significantly higher. These data demonstrate the importance of optimizing the composition and structure of sebum, reducing lipid peroxides, and preventing sebum peroxidation in preventing acne inflammation, which may be an effective treatment for recurrent acne.

[0008] Based on the above research, the present invention provides an acne-removing composition. By adding mango leaf extract, guaiac extract, acetyl glucosamine, yeast extract and EUK-134 in an appropriate mass range, the five ingredients can cooperate with each other to optimize the sebum component as a whole, maintain the stability of the ratio of saturated fatty acids to unsaturated fatty acids in the sebum component, and inhibit sebum peroxidation, thereby achieving the effects of mild acne removal and repairing the sebum layer barrier. In addition, the acne-removing composition provided by the present invention has excellent antibacterial properties. When the acne-removing composition is used as an active ingredient in the preparation of cosmetics, the addition of antibacterial agents can be avoided, further improving the mildness of the product.

[0009] Specifically, mango leaf extract has a certain inhibitory effect on sebum secretion. While specifically inhibiting the unsaturated fatty acids in sebum that cause inflammation and acne, it has no effect on stable components like cholesterol and waxes. This optimizes sebum composition, stabilizes the sebum membrane, prevents acne, and repairs the skin barrier. Guaiac wood extract is rich in phenols, lignin, quinones, saponins, sesquiterpenes, and triterpenes, which have anti-inflammatory, antiviral, antibacterial, astringent, and repairing properties. It can inhibit the inflammation caused by excess oil in the sebaceous glands, which can lead to inflammatory acne, and promote the healing of acne scars and pits. Acetyl glucosamine, a derivative of glucose found in nearly all organisms, promotes the synthesis of hyaluronic acid, providing potent moisturizing and repairing properties. It also cleaves the CD44 gene, a "hinge" between cells, reducing cell adhesion, softening keratin, and gently exfoliating old cells. Yeast extract, an active ingredient isolated from plant yeast using bio-extraction technology, has antioxidant and free radical protection, promotes metabolism, and moisturizes the skin, inhibiting sebum oxidation for a soft and delicate appearance. EUK-134 possesses the properties of SOD and catalase, possessing strong antioxidant capacity. It catalyzes superoxide anion radicals to form hydrogen peroxide, repairing oxidative damage to the skin and inhibiting inflammatory responses caused by free radicals. It is highly stable and renewable.

[0010] As a preferred embodiment of the anti-acne composition of the present invention, the anti-acne composition comprises the following components in parts by weight: 0.5-3 parts of mango leaf extract, 1-5 parts of guaiac extract, 0.2-3 parts of acetyl glucosamine, 0.1-3 parts of yeast extract, and 0.01-1 parts of EUK-134.

[0011] As a preferred embodiment of the anti-acne composition of the present invention, the anti-acne composition comprises the following components in parts by weight: 0.8-1.5 parts of mango leaf extract, 1.2-3 parts of guaiac extract, 0.3-1 parts of acetyl glucosamine, 0.5-1.5 parts of yeast extract, and 0.01-0.5 parts of EUK-134.

[0012] As a preferred embodiment of the anti-acne composition of the present invention, 1 part of mango leaf extract, 1.5 parts of guaiac extract, 0.5 parts of acetyl glucosamine, 1 part of yeast extract, and 0.1 parts of EUK-134.

[0013] The inventors have discovered that changes in the number of components added to the anti-acne composition directly affect the product's stabilization and optimization of sebum in many aspects, and also affect the product's antioxidant, acne-removing, and antibacterial properties. Simultaneously, the inventors discovered during experiments that the effect of gradually increasing or decreasing the content of a single component on the overall product effect is not linear. When the number of components added is further selected within the preferred range of the present invention, the resulting product achieves the best overall effect.

[0014] As a preferred embodiment of the anti-acne composition of the present invention, the mass percentage of mangiferin in the mango leaf extract is 80-90%.

[0015] The mango leaf extract preparation method of the present invention comprises: washing mango leaves and airing them until dry, then adding 50% ethanol (the same weight as the raw material), placing them in a fractionation apparatus, performing directional fractionation extraction at 60-85°C, and simultaneously adding an equal weight of 1-butyl-3-methylimidazolium chloride ionic liquid, stirring and mixing at room temperature for 1.5-2.5 hours, standing and separating the layers, and subjecting the ionic liquid layer to vacuum distillation to obtain a mango leaf extract enriched in mangiferin. Using the above extraction method, by adjusting the extraction parameters, the mass percentage of mangiferin in the mango leaf extract can be adjusted between 65-95%. The high content of mangiferin in the extract has anti-inflammatory and antioxidant effects, but the inventors have found that if pure mangiferin is used instead of mango leaf extract, the resulting product is not as effective as using mango leaf extract. The inventors have found that when the mass percentage of mangiferin in the mango leaf extract is further preferably 80-90%, the resulting product has better antioxidant and sebum stabilizing effects.

[0016] As a preferred embodiment of the anti-acne composition of the present invention, in the anti-acne composition, the mass percentage of mangiferin is 18-30%, and the mass percentage of guaiac extract is 30-45%.

[0017] Preferably, in the anti-acne composition, the mass percentage of mangiferin is 20-25%, and the mass percentage of guaiac extract is 35-38%.

[0018] The extraction process of the guaiac wood extract of the present invention is as follows: weigh the guaiac wood, crush it into powder, add 60% to 95% ethanol aqueous solution in a volume 5 to 10 times the weight of the guaiac wood, stir and extract 1 to 3 times at 60 to 80° C., filter, combine the filtrate, and concentrate under reduced pressure to obtain the guaiac wood extract.

[0019] The guaiac extract prepared by the above-mentioned extraction method contains a variety of substances with antibacterial effects, such as dihydroguaiac acid, guaiac alcohol, brusinol, guaiacol, etc. The inventors have found that mangiferin, in addition to its own antibacterial properties, can also enhance the antibacterial properties of the guaiac extract to a certain extent, that is, it has a certain synergistic effect on the antibacterial properties of the guaiac extract; when the mass percentages of mangiferin and guaiac extract in the acne-removing composition are within the preferred range, the antibacterial properties of the obtained product are even better.

[0020] As a preferred embodiment of the anti-acne composition of the present invention, the mass percentage of acetyl glucosamine in the anti-acne composition is 12-13%.

[0021] Acetyl glucosamine is a product obtained by enzymatic extraction, and its substrate is glucose. Due to its cationic properties, it can synergize with guaiac extract and mango leaf extract to achieve excellent and long-lasting antibacterial effects. The inventors have found that when the mass percentage of acetyl glucosamine in the acne-removing composition is further selected within the above range, it can better interact with guaiac extract and mango leaf extract to achieve a more excellent antibacterial effect.

[0022] In a second aspect of the present invention, the present invention provides a method for preparing the anti-acne composition, which comprises the following steps: mixing the components to obtain the anti-acne composition.

[0023] The preparation method of the acne-removing composition provided by the present invention is simple and conventional and does not require any special equipment.

[0024] In a third aspect of the present invention, the present invention provides use of the anti-acne composition in cosmetics.

[0025] In a fourth aspect of the present invention, the present invention provides a cosmetic comprising the anti-acne composition of the present invention.

[0026] As a preferred embodiment of the cosmetic of the present invention, the mass percentage of the anti-acne composition of the present invention in the cosmetic is 0.5-5%.

[0027] When the anti-acne composition provided by the present invention is added as an effective component to the preparation of cosmetics at the above-mentioned mass percentage, the resulting cosmetics have the effects of optimizing sebum components, maintaining the stability of the ratio of saturated fatty acids to unsaturated fatty acids in the sebum components, inhibiting sebum peroxidation, gently removing acne, and repairing the sebum layer barrier. In addition, without adding antibacterial agents, it can also achieve the effect of effectively inhibiting the proliferation of related bacteria and fungi, and the effect is long-lasting. If the content of the anti-acne composition is further increased, the improvement effect on the effect is not obvious.

[0028] As a preferred embodiment of the cosmetic of the present invention, the cosmetic further comprises at least one of a thickener, a moisturizer, an emulsifier, a preservative, an essence, a pH adjuster and water.

[0029] As a preferred embodiment of the cosmetic of the present invention, the cosmetic comprises the following components in percentage by mass: 0.5-5% of the anti-acne composition of the present invention, 0.1-0.5% of a thickener, 4-20% of a moisturizer, 0.1-10% of an emulsifier, 0.5-5% of a preservative, 0.01-0.2% of a fragrance, 0.05-0.1% of a pH adjuster, and the remainder being water.

[0030] Preferably, the thickener includes at least one of xanthan gum, carbomer 940, AVC, and high molecular weight cellulose; the moisturizer includes at least one of glycerin, butylene glycol, and low molecular weight sodium hyaluronate; the emulsifier includes at least one of coconut oil caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane; the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and parahydroxyacetophenone; and the pH adjuster includes at least one of arginine and EDTA-2Na.

[0031] As a preferred embodiment of the cosmetics of the present invention, the cosmetics include any one of lotion, emulsion, cream, mask, essence, sunscreen, and spray.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] The present invention provides an anti-acne composition for optimizing skin oil quality. The five ingredients in the anti-acne composition cooperate with each other to optimize the sebum composition as a whole within a given range, maintain a stable ratio of saturated fatty acids to unsaturated fatty acids in the sebum components, inhibit sebum peroxidation, and achieve the effects of mild acne removal and repairing the sebum barrier. Furthermore, the anti-acne composition provided by the present invention has excellent antibacterial properties. Furthermore, the anti-acne composition provided by the present invention can be added as an active ingredient in the preparation of cosmetics, avoiding the addition of antibacterial agents in the cosmetics and achieving mild and non-irritating cosmetics. Furthermore, the preparation method of the anti-acne composition of the present invention is simple, the raw materials are readily available, and it is easy to produce in practice. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] FIG1 is a diagram showing the test results of the facial visual performance of the emulsion of Example 10 in Effect Example 5;

[0035] FIG2 is a graph showing the HPLC detection results of squalene content in facial sebum of the emulsion of Example 10 in Effect Example 5. DETAILED DESCRIPTION

[0036] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0037] Unless otherwise specified, the reagents, methods, and equipment used in the present invention are all conventional reagents, methods, and equipment in the art; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0038] Mango leaf extract 1: The mass percentage of mangiferin is 90%;

[0039] Mango leaf extract 2: The mass percentage of mangiferin is 80%;

[0040] Mango leaf extract 3: The mass percentage of mangiferin is 70%;

[0041] Acetyl glucosamine: Jiangsu Aoxin Bioengineering Co., Ltd.;

[0042] Yeast extract: commercially available.

[0043] Examples 1-9 and Comparative Examples 1-11

[0044] Examples 1-9 and Comparative Examples 1-11 of the present invention provide an anti-acne composition, the component contents (parts by mass) of which are shown in Table 1;

[0045] Table 1

[0046] The preparation method of the anti-acne composition provided in Examples 1-9 and Comparative Examples 1-11 is: uniformly mixing the components to obtain the anti-acne composition.

[0047] Comparative Example 12

[0048] The comparative example of the present invention provides an anti-acne composition, the only difference between which is compared with Example 1 is that mangiferin is used instead of mango leaf extract 1.

[0049] Comparative Example 13

[0050] The comparative example of the present invention provides an acne-removing composition, the only difference between which is compared with Example 1 is that the Centella asiatica extract is used instead of the Guaiac extract.

[0051] Comparative Example 14

[0052] The comparative example of the present invention provides an anti-acne composition, the only difference between which is compared with Example 1 is that chitosan is used instead of acetyl chitosan amine.

[0053] Comparative Example 15

[0054] The comparative example of the present invention provides an anti-acne composition, the only difference between which is compared with Example 1 is that the yeast extract is replaced by a lactobacillus fermentation product.

[0055] Comparative Example 16

[0056] The comparative example of the present invention provides an anti-acne composition, the only difference between which is compared with Example 1 is that vitamin C is used instead of EUK-134.

[0057] Examples 10-18 and Comparative Examples 17-32

[0058] Examples 10-18 of the present invention and comparative examples 17-32 provide an emulsion, the components and mass percentages of the emulsion are shown in Table 2;

[0059] Table 2

[0060] The acne-removing compositions in Examples 10-18 are respectively the acne-removing compositions prepared in Examples 1-9, and the acne-removing compositions in Comparative Examples 17-32 are respectively the acne-removing compositions prepared in Comparative Examples 1-16;

[0061] The preparation methods of the emulsions provided in Examples 10-18 and Comparative Examples 17-32 are as follows:

[0062] (1) Component A and water were mixed and stirred, heated to 75°C, and homogenized at 1200 rpm for 5 minutes. After homogenization, the mixture was kept warm for later use to obtain prefabricated component A;

[0063] (2) Component B was mixed, heated to 75°C, and homogenized at 1200 rpm for 5 minutes. After homogenization, the mixture was kept warm for later use to obtain prefabricated component B.

[0064] (3) Mix the C components and heat to 60°C to melt to obtain a prefabricated C component;

[0065] (4) The preformed component A was heated to 80°C, and the preformed component B was added at a speed of 800 rpm, and stirred and mixed. Then, the temperature was lowered to 60°C, and the preformed component C was added at a speed of 400 rpm, and stirred and mixed. Then, the temperature was lowered to below 40°C, and the anti-acne composition was added and continued to be stirred for 10 minutes. Finally, arginine was added to adjust the pH, and the material was discharged to obtain an emulsion.

[0066] Example 19

[0067] This embodiment of the present invention provides an emulsion. The only difference between the emulsion and Example 10 is that the added mass percentage of the anti-acne composition is 0.5%.

[0068] Example 20

[0069] This embodiment of the present invention provides an emulsion. The only difference between the emulsion and Example 10 is that the added mass percentage of the anti-acne composition is 8%.

[0070] Example 21

[0071] This embodiment of the present invention provides an emulsion, the only difference between the emulsion and Example 10 is that no preservative is added.

[0072] Effect Example 1

[0073] This effect example explores the safety of the acne-removing compositions in Examples 1-9 and Comparative Examples 1-16, using a chicken chorioallantoic membrane test. This procedure is performed in accordance with the People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard SN / T 2329-2009 "Chicken chorioallantoic membrane test for eye irritation / corrosion of cosmetics." Specifically, the following steps are included:

[0074] (1) Use tweezers to peel open the eggshell from the air chamber end of a 9-day-old egg, soak the inner membrane with 0.9% saline, and then carefully peel off the egg membrane from the edge to reveal the chorioallantoic membrane;

[0075] (2) Using a pipette, 0.3 mL of the test samples of Examples 1-9 and Comparative Examples 1-16 were taken, dissolved in PBS (pH 7.0), and diluted to a volume fraction of 1% before testing;

[0076] (3) After 3 minutes, observe the vascular changes of the chorioallantoic membrane. Transparent test substances can be directly photographed. Turbid test substances or other test substances that affect observation can be washed away with 0.9% saline before photographing. Observe the silicone rubber ring and the chick embryo chorioallantoic membrane using a magnifying glass lamp. Vascular congestion, vascular hemorrhage, and ghost vessels indicate irritation reactions.

[0077] If observations indicate that at least one reaction in all six chicken embryos is scored as moderate or above (total score ≥ 12), the test should be repeated. Each chicken embryo score = the sum of the degree of bleeding, coagulation, and vascular lysis observed in each chicken embryo; ES = the average of the mathematical sum of the scores of the six chicken embryos. Each sample is tested in parallel six times. The chicken embryo test criteria (safety endpoint evaluation criteria) are shown in Table 3.

[0078] Table 3

[0079] The statistical results show that the ES values ​​of the acne-removing compositions of Examples 1-9 and Comparative Examples 1-16 are all ≤12, that is, all the acne-removing compositions are non-irritating or mildly irritating and have good safety.

[0080] This effect example explores the antioxidant properties of the acne-removing compositions in Examples 1-9 and Comparative Examples 1-16, using a DPPH free radical scavenging experiment, specifically comprising the following steps:

[0081] (1) Dissolve 0.001972 g of DPPH in anhydrous ethanol solution, dilute to 25 mL with anhydrous ethanol solution, measure the absorbance at 517 nm, and record the absorbance value between 200 and 800 to obtain the DPPH test solution;

[0082] (2) Using Examples 1-9 and Comparative Examples 1-16 as test samples, pipette 0.1 mL of each sample solution into PBS to prepare a 1% sample test solution. Prepare the solutions in tubes A, B, and C as follows, incubate at room temperature in the dark for 30 minutes, measure the absorbance at 517 nm, and record the absorbance value. Repeat three times for each sample.

[0083] Tube A: 2 mL sample + 2 mL DPPH test solution;

[0084] Tube B: 2 mL DPPH test solution + 2 mL PBS;

[0085] Tube C: 2 mL PBS + 2 mL sample;

[0086] Zero adjustment: anhydrous ethanol solution;

[0087] The calculation method is: DPPH free radical scavenging rate = 1-(AC) / B×100%. The experiment was repeated 3 times and the average value was taken. The test results are shown in Table 4.

[0088] Table 4

[0089] As can be seen from Table 4, when the technical solution of the present invention is adopted, the obtained acne-removing composition has excellent in vitro DPPH scavenging ability, specifically, the scavenging rate is above 61.47%. As can be seen from Examples 1-7 and Comparative Examples 1-11, the weight ratio of the acne-removing composition has a significant impact on the performance of the product. When the number of components added in Examples 1-3 is further selected, the obtained acne-removing composition has a stronger DPPH scavenging ability, exceeding 83.27%.

[0090] As can be seen from Example 1 and Comparative Examples 1-10, whether only one of the five ingredients is added or not, the DPPH scavenging ability of the obtained product is significantly reduced. Compared with Example 1, the reduction in the clearance rate is between 48.02% and 65.35%. As can be seen from Example 1 and Comparative Examples 12-16, when the five components of the present invention are replaced by other components with the same functions, the DPPH scavenging rate of the obtained product is also significantly reduced. Compared with Example 1, the reduction in the clearance rate is between 48.06% and 72.00%. It can be clearly seen from the above data that the five components in the anti-acne composition provided by the present invention have a good synergistic effect and can achieve excellent DPPH scavenging effect.

[0091] Effect Example 3

[0092] This effect example explores the antibacterial properties of the acne-removing compositions in Examples 1-9, Comparative Examples 1-3, and Comparative Examples 12-14, and the antibacterial properties of the emulsions in Examples 10 and 21.

[0093] Staphylococcus aureus (ATCC6538) is a Gram-positive bacterium, Escherichia coli (ATCC8739) is a Gram-negative bacterium, and Candida albicans (ATCC10231) is a fungus. They were purchased from the Guangdong Center for Microbiological Culture Collection. Staphylococcus aureus and E. coli were activated in Luria-Bertani (LB) medium at 37°C, while Candida albicans was activated in Sandburg agar at 37°C. The antibacterial effect of each sample was determined according to "7.3 Antibacterial Effect Test Method for Antibacterial Daily Chemical Products (Suspension Quantitative Method)" in "QBT 2738-2018 Evaluation Method for Antibacterial and Bacteriostatic Effect of Daily Chemical Products." The determination time was 20 minutes. The antibacterial rate (%) results are shown in Table 5.

[0094] Table 5

[0095] As can be seen from Table 5, the antibacterial properties of the acne-removing compositions obtained using the technical solution of the present invention are extremely excellent. Under the experimental conditions, no Escherichia coli, Staphylococcus aureus, and Aspergillus niger were detected, and the overall appearance of the acne-removing composition did not change, that is, it had excellent stability. Furthermore, when the mass percentage of mangiferin in Examples 1-3 and Example 5 was between 18-30% and the mass percentage of guaiac extract was between 30-45%, the antibacterial properties of the obtained acne-removing compositions were even better. Within the same period of time, the detected levels of Escherichia coli, Staphylococcus aureus, and Candida albicans were lower. In particular, in Example 1, when the mass percentage of mangiferin was 20-25%, the mass percentage of guaiac extract was 35-38%, and the mass percentage of acetyl glucosamine was 12-13%, the obtained acne-removing composition had the lowest levels of Escherichia coli, Staphylococcus aureus, and Candida albicans detected within the same period of time compared to other acne-removing compositions. At the same time, it can be seen from Example 1 and Comparative Examples 1-2 that when mango leaf extract and guaiac extract are not added to the components, the obtained product has no antibacterial effect; it can be seen from Example 1 and Comparative Example 3 that when acetyl glucosamine is not added, the antibacterial effect of the obtained product is significantly reduced, indicating that acetyl glucosamine, guaiac extract and mango leaf extract have a synergistic antibacterial effect, and can achieve maximum antibacterial efficiency when used in combination; this can also be seen from Example 1 and Comparative Examples 12-14 that when other similar ingredients are used to replace acetyl glucosamine, guaiac extract and mango leaf extract, the antibacterial efficiency of the obtained product is also significantly reduced.

[0096] As can be seen from Examples 10 and 21, when the anti-acne composition of the present invention is used as an active ingredient in the preparation of an emulsion, even without adding preservatives, a good effect of inhibiting the proliferation of related bacteria can be achieved, thereby reducing the addition of preservatives to a certain extent and further improving the mildness of the product.

[0097] Effect Example 4

[0098] This effect example tests the effects of Example 1 and Comparative Examples 1-6, Comparative Examples 11-12, and Comparative Examples 14-15 on the lipid secretion of SZ95 cells in vitro. The specific steps are as follows:

[0099] SZ95 cells were activated and cultured in Sebomed culture medium containing 10% fetal bovine serum, 500 U / mL penicillin, 500 U / mL streptomycin, and 5 ng / mL epidermal growth factor at 37°C in a saturated humidity environment with 5% CO2. Example 1 and Comparative Examples 1-6, 11-12, and 14-15 to be tested were diluted with DMSO to a concentration of 0.1% by volume.

[0100] The cell suspension was inoculated into a 96-well cell culture plate (black bottom transparent type), with 1.0×10 4 Cells were cultured for 24 hours. After 24 hours of incubation, the supernatant was discarded. The blank control group was added with 100 μL of maintenance medium, the model control group was added with 100 μL of maintenance medium containing 10 ng / mL hydrocortisone, and the positive control group was added with 100 μL of 10 ng / mL hydrocortisone and 0.01 mM isotretinoin solution. The experimental groups were added with 100 μL of 10 ng / mL HC and 0.1% of each test sample. After 48 hours of incubation, the supernatant was discarded and the cells were washed twice with PBS. The experimental group was stained with 100 μL of 10 μg / mL Nile Red dye, and the control group was stained with 15 μg / mL fluorescein diacetate (FDA). After incubation at 37°C in the dark for 5 minutes, the fluorescence intensity was measured on a multifunctional microplate reader with excitation wavelengths of 485 nm, 494 nm, and 565 nm and absorption wavelengths of 523 nm. The results were determined using the ratio of Nile Red to FDA: intracellular neutral lipid percentage (%) = experimental group OD ratio / control group OD ratio x 100%. Each test was repeated three times, and the results are shown in Table 6.

[0101] Table 6

[0102] Note: “#” indicates that there is a significant difference between the blank control group and the model group, P < 0.05; “*” indicates that there is a significant difference between the sample group and the model group, P < 0.05.

[0103] As can be seen from Table 6, the relative content of neutral lipids in the model group was significantly increased compared with the blank group (P < 0.005), indicating that the model was successful, and the relative content of neutral lipids in the positive control group was significantly decreased compared with the model control group (P < 0.005), indicating that the experiment was effective. At a concentration of 0.1%, Example 1 has the strongest effect of inhibiting the secretion of oil by SZ95 cells; the data of Comparative Examples 1-6 show that the five acne-removing compositions of the present invention have a synergistic effect, and increasing or decreasing any of the components will reduce the effect of inhibiting the secretion of neutral lipids; the data of Comparative Example 11 shows the scientific nature of considering the content range of the acne-removing composition of the present invention; the data of Comparative Examples 12 and Comparative Examples 14 and 15 show that the acne-removing composition of the present invention is not an alternative in inhibiting oil secretion.

[0104] Effect Example 5

[0105] This effect example tests the effects of the emulsions of Example 10 and Comparative Examples 17, 20, 22, and 28 on human bodies, and simultaneously sets up a blank test. The only difference between the blank test and Example 10 is that the anti-acne composition of the present invention is not added. Specifically, the following steps are included:

[0106] 1. Facial intuitive expression test

[0107] Select patients aged 18-45 years old, with a facial oil content of no less than 120μg / cm 2 , 48 volunteers tested positive for lactic acid sting. The day before the visit, the volunteers washed their faces without applying any skin care products. On the day of the visit, the volunteers washed their faces with clean water without applying any products. They sat quietly in an air-conditioned room at a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. The initial facial oil content was tested using the skin oil detection probe Sebumeter SM815, and the skin oil content was tested using Sebutapes TM The skin keratin sampling patch is used to sample the forehead. After cleansing the face, the patient sits quietly in the test room for more than 20 minutes. A professional doctor counts the acne, papules, and blackheads on the nose, scores the degree of facial redness, and uses Visia-CR to take facial photos. The red zone images derived under cross-polarized light are analyzed to obtain the quantitative indicator red zone analysis a* value. The skin transepidermal water loss probe is used to measure the red zone. Hex (Courage+Khazaka) tests the initial facial TEWL value. The subjects were randomly divided into 6 groups, with 8 people in each group. The blank group, Example 10, Comparative Example 17, Comparative Example 20, Comparative Example 22, Comparative Example 28 and the blank test emulsion were randomly used on the whole face, once in the morning and once in the evening. Facial oil, acne, papules, blackheads, TEWL, Visia-CR were tested and photographed on the 7th, 14th and 28th days, and forehead oil was sampled. The facial oil content, TEWL improvement, acne and blackhead improvement, redness improvement, and a* value improvement of each volunteer on days 0, 7, 14 and 28 were analyzed. The improvement is represented by the improvement rate of each numerical value, and the calculation method is as follows:

[0108] The obtained data are shown in Table 7.

[0109] Table 7

[0110] Note: “*” indicates that the improvement rate at each time point is significantly different from the initial value, P < 0.05.

[0111] As can be seen from Table 7, when the technical solution of the present invention is adopted, the symptoms of facial skin can be effectively improved compared with the blank group. When the emulsion in Example 10 is used for testing, a typical facial picture of the subject is shown in Figure 1. It can also be intuitively seen from Figure 1 that the emulsion in Example 10 can significantly improve the facial condition; further, it can be seen from Example 10 and Comparative Example 28 that the type of component selection will have a significant impact on the performance of the product. In particular, when the emulsion of Example 10 is prepared by further selecting the anti-acne composition in Example 1 as the active component, the improvement effect on the facial condition is more significant, wherein the change rate of oil at 14 days is more than 35.7%, the change rate of TEWL at 14 days is more than 17.7%, the change rate of acne at 14 days is more than 17.0%, the change rate of papules at 14 days is more than 37.0%, the change rate of blackheads at 14 days is more than 22.6%, the change rate of red area at 14 days is more than 77.8%, and the change rate of a* value at 14 days is more than 25.8%.

[0112] As can be seen from Example 10, Comparative Example 20, and Comparative Example 22, when the mass percentage of the anti-acne composition in the emulsion remains unchanged, but the ingredients in the anti-acne composition are changed, whether one component is omitted, only one component is added, or other substances with similar functions are substituted, the improvement effect of the resulting product on facial condition is significantly reduced.

[0113] 2. Sebum component detection test

[0114] Skin keratin patches collected from volunteers at 0, 7, 14, and 28 days of age were extracted with ethanol containing 0.025% butylated hydroxytoluene. Liquid-liquid extraction with ethyl acetate was performed to further remove impurities. The extract was dissolved in a mixture of acetone / methanol / isopropanol (40 / 40 / 20 v / v) to a concentration of 5 mg / mL and stored at −80°C until analysis.

[0115] The lipid peroxide (LPO) level was determined using the instructions for the Nanjing Jiancheng test kit (catalog number BC5240). LPO is heated under acidic conditions to produce malondialdehyde, which condenses with thiobarbituric acid (TBA) to form brown-red trimethine (3,5,5-trimethyloxazole-2,4-dione), which has a maximum absorption peak at 535 nm. To eliminate the influence of the absorbance of interfering products, the absorbance at 600 nm was subtracted.

[0116] The content of squalene in sebum was determined by HPLC-MS.

[0117] The results are shown in Table 8;

[0118] Table 8

[0119] As can be seen from Table 8, from a molecular perspective, the emulsion obtained by the technical solution of the present invention can significantly optimize the composition ratio of sebum, reduce the proportion of lipid peroxides and unsaturated fatty acids, and maintain sebum in a normal skin state; specifically, the LPO value of the product obtained by the technical solution of the present invention decreased by 0.17 nmol / cm after 14 days of use. 2 , squalene decreased by 1.52μg / cm 2 When the emulsion in Example 10 was used for testing, the HPLC content detection chart of squalene content obtained was shown in Figure 2. It can be seen intuitively from Figure 2 that the content of squalene was significantly reduced.

[0120] It can be seen from Example 10, Comparative Example 20, Comparative Example 22, and Comparative Example 27 that when the mass percentage of the anti-acne composition in the emulsion remains unchanged, but the ingredients in the anti-acne composition are changed, whether one component is omitted, only one component is added, or other substances with similar functions are substituted, the ability of the resulting product to change squalene and LPO in sebum is significantly reduced.

[0121] Finally, it should be noted that the above embodiments are intended to illustrate the technical solutions of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-acne composition for optimizing skin oil quality, characterized in that: The anti-acne composition comprises the following components in parts by weight: 0.1-5 parts of mango leaf extract, 0.1-10 parts of guaiac extract, 0.1-10 parts of acetyl glucosamine, 0.1-10 parts of yeast extract, and 0.01-5 parts of EUK-134.

2. The anti-acne composition according to claim 1, characterized in that: The anti-acne composition comprises the following components in parts by weight: 0.5-3 parts of mango leaf extract, 1-5 parts of guaiac extract, 0.2-3 parts of acetyl glucosamine, 0.1-3 parts of yeast extract, and 0.01-1 parts of EUK-134.

3. The anti-acne composition according to claim 2, characterized in that: The anti-acne composition comprises the following components in parts by weight: 0.8-1.5 parts of mango leaf extract, 1.2-3 parts of guaiac extract, 0.3-1 parts of acetyl glucosamine, 0.5-1.5 parts of yeast extract, and 0.01-0.5 parts of EUK-134.

4. The anti-acne composition according to claim 3, characterized in that: The anti-acne composition comprises the following components in parts by weight: 1 part of mango leaf extract, 1.5 parts of guaiac extract, 0.5 parts of acetyl glucosamine, 1 part of yeast extract, and 0.1 parts of EUK-134.

5. The anti-acne composition according to claim 1, characterized in that: In the mango leaf extract, the mass percentage of mangiferin is 80-90%.

6. The anti-acne composition according to claim 1, characterized in that: In the anti-acne composition, the mass percentage of mangiferin is 18-30%, and the mass percentage of the guaiac extract is 30-45%.

7. The anti-acne composition according to claim 6, characterized in that: In the anti-acne composition, the mass percentage of mangiferin is 20-25%, and the mass percentage of the guaiac extract is 35-38%.

8. The anti-acne composition according to claim 6, characterized in that: In the anti-acne composition, the mass percentage of acetyl glucosamine is 12-13%.

9. The method for preparing the anti-acne composition according to any one of claims 1 to 8, characterized in that: The preparation method comprises the following steps: mixing the components to obtain the anti-acne composition.

10. Use of the anti-acne composition according to any one of claims 1 to 8 in cosmetics.

11. A cosmetic, characterized in that: The cosmetic comprises the anti-acne composition according to any one of claims 1 to 8.

12. The cosmetic according to claim 11, characterized in that In the cosmetic, the mass percentage of the anti-acne composition according to any one of claims 1 to 8 is 0.5-5%.

13. The cosmetic according to claim 11, characterized in that The cosmetics further include at least one of a thickener, a moisturizer, an emulsifier, a preservative, an essence, a pH adjuster and water.

14. The cosmetic according to claim 13, characterized in that The cosmetics include the following components in percentage by mass: 0.5-5% of the anti-acne composition according to any one of claims 1 to 8, 0.1-0.5% of a thickener, 4-20% of a moisturizer, 0.1-10% of an emulsifier, 0.5-5% of a preservative, 0.01-0.2% of a flavor, 0.05-0.1% of a pH adjuster, and the remainder being water.

15. The cosmetic according to claim 11, characterized in that: The cosmetics include any one of lotion, emulsion, cream, mask, essence, sunscreen and spray.

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