Herbal burn cream and herbal burn cream production method

Abstract

The invention is particularly related to an herbal burn cream that increases the antimicrobial, antifungal, antioxidant and cell proliferation effects and reduces side effects by using hypericum perforatum (St. John's wort) and energized oxygen in burn / wound treatment, and the production method that enables this cream to be obtained.

Description

[0001] HERBAL BURN CREAM AND HERBAL BURN CREAM PRODUCTION METHOD

[0002] Technical Field

[0003] The invention relates to an herbal burn cream and production method that provides increased antimicrobial, antifungal, antioxidant and cell proliferation effects.

[0004] In particular, the invention relates to an herbal burn cream for the treatment of burns / wounds using Hypericum perforatum (St. John's wort) and energized oxygen to increase antimicrobial, antifungal, antioxidant and cell proliferation effects and to reduce side effects, and to a production method for obtaining such cream.

[0005] State of the Art

[0006] Skin, known as the largest organ of our body, performs many functions necessary for the organism to maintain its vitality. Different types of wounds occur as a result of various internal and external factors and disruptions in skin integrity. Organisms tend to close these wounds as quickly and effectively as possible. In this healing process, which consists of hemostasis, inflammation, proliferation and maturation phases, devitalized and lost cellular structures and tissue layers are reconstructed through various biochemical and cytological processes. Burns, which are among the wound groups, are defined as damage to body tissues caused by factors such as light, chemicals, radiation effect, etc. Burns are classified in different ways according to their depth, the width of the area they cover, and the factors that cause the burn. However, the most commonly used classification today is the depth classification, which takes into account the damage to the skin layers of the burn. Burns according to their depth; They are examined in four separate groups as first, second-, third- and fourth-degree burns.

[0007] First degree burns; It manifests itself as hyperemia and is mild and superficial. In first-degree burns, where the existing damage is limited to the epidermis, some losses occur in the skin's barrier functions. However, these burns usually heal spontaneously with the regeneration of the epidermis within 5-10 days and do not cause scarring. Methods such as cold application, topical preservatives and medication are used as treatment methods. In second-degree burns, damage is seen in the epidermis, the uppermost layer of the skin, and a part of the dermis. While this damage is sometimes limited to only the superficial part of the dermis, sometimes it can extend to the reticular dermis. Although the symptoms of these burns are different; Redness, blister formation, swelling, pain and color change are usually observed in the burn area. Healing in these burns occurs through re-epithelialization of keratinocytes in the hair follicle and sweat glands. Although the healing process usually varies between 10-35 days, it may require surgical intervention. Third degree burns; It is defined as burns in which all layers of the skin lose their vitality. Although color change is observed in the burn area, there is no pain. The skin is quite hard and loses its elasticity. In these burns, re-epithelialization is generally not possible because the keratinocytes in the stratum germinativum, hair follicle and sweat glands lose their vitality. Scar formation is observed in this type of burns and some cases require surgical intervention. Fourth degree burns; There are burns that are life-threatening and involve all layers of the skin and subcutaneous tissues such as fascia, muscle and bone. Characteristically, dryness, hardening and loss of sensation are observed along with skin color change (usually brown or black). As a treatment method, all tissues that have lost their vitality are first debrided. Tissue reconstruction is then performed. Today, wound healing is carried out on the basis of the modern wound care approach that emerged with Winter's study on the "healing effect of moisture in wound healing" in 1962. The main thing in this modern understanding is to evaluate the wound correctly, determine its needs and provide ideal environmental conditions during the wound healing process. Various medical materials are used in tissue regeneration of burns. Materials used for tissue regeneration can be in the form of hydrogels, foams, transparent films, creams and wound dressings.

[0008] Table 1. Classification of burns according to their depth and their characteristics chart Wound-burn creams, which are among the products used in tissue regeneration; They are widely used because they have advantages such as their solubility, effective active ingredient release, and easy application to the wound-burn area. Ozone (O3), used in some of these products, is a gas composed of three oxygen atoms. Ozone is an extremely unstable molecule formed by adding an oxygen atom to the diatomic oxygen molecule (O2), and it is commercially produced by passing oxygen molecules (O2) through an electric field using the corona current method. Since ozone is a gas with an extremely high oxidation capacity, it is used for disinfection and removal of organic / inorganic substances. The general properties of ozone / tri-oxygen (O3) are that it is located in the troposphere layer at the junction with the stratosphere, it contains 3 oxygen atoms in a single molecule, it has a severe oxidizing effect and provides nitrogen to the protein synthesis of plants through nitrogen-containing compounds formed by oxidizing nitrogen in the air, and it is produced artificially. Tri-oxygen / ozone molecules cause the formation of NXOX, SXOXand toxic organic compounds in the air.

[0009] However, when ozone / tri-oxygen (O3) is released into the air, it creates N2O5 derived nitrogen oxide, causing lesions in the lungs. When inhaled, it triggers nausea and vomiting. It has a catalyzing effect on stomach acid by reacting with water and converting H2O into H2O2. As a result, when taken orally, dissolved in water or oil, it causes stomach sores and reflux. In addition, it does not have a sufficient effect on disinfection and is not completely effective against bacteria and viruses in sterilization applications. However, it is not suitable to be used in the sterilization of pharmaceutical liquids as it causes hydrogen peroxide formation and the ozone oils obtained are in liquid form since their adhesion rate in oil is low. To increase consistency, use base, cream or petroleum jelly etc. It needs to be supplemented with water and when applied pure to the skin, its absorption by the skin is low. For this reason, ozone-containing creams on the market generally use chemical ingredients and external additives, which increases the side effects of the resulting creams on the user and causes undesirable conditions, while also reducing the effectiveness of healing.

[0010] Hypericum perforatum (St. John's Wort), which is among the medicinal and aromatic plants used in similar treatment areas and is known for its wound / burn healing properties, is a preferred wound healing agent with its antimicrobial and antioxidant effects. Current studies show that the wound healing activity of Hypericum perforatum is due to fibroblastic activity and collagen increase. However, the use of Hypericum perforatum alone is not sufficient to quickly heal wound groups, especially second-degree burns, and to protect the burn area against infections by increasing antimicrobial-antioxidant effects. A prior art patent document numbered CN106176876A discloses a traditional Chinese medicine and preparation method containing an oil extraction to promote trauma healing. This traditional medicine is prepared by using 2.5% St. John's wort oil extract, 0.5-5% vitamin E and olive oil by mass. This drug has advantages such as easy absorption, waterproofness and high air permeability. In addition, it can provide inhibition of microorganisms that may cause infection, cell activation and repair of damaged cells, and have hemostasis and analgesic effects. In the document in question, there is no mention of a solution to obtain a cream that increases the antimicrobial, antifungal, antioxidant and cell proliferation effects and reduces side effects by using energized oxygen together with Hypericum perforatum in burn / wound treatment.

[0011] In a patent document numbered CN112867540A belonging to the known technique, a phytotherapeutic composition obtained with four natural oils consisting of appropriately mixed Hypericum perforatum oil, Prunus amygdalus dulcis oil, Calendula officinalis oil, Echinacea angustifolia oils is described. Ozone, which has antiviral and antibacterial effects, is used in the creation of this composition. The composition contributes to tissue and bone healing and can be used especially during post-operative recovery and long-term hospital stays. In the document in question, there is no mention of a solution to obtain a cream that increases the antimicrobial, antifungal, antioxidant and cell proliferation effects and reduces side effects by using energized oxygen together with Hypericum perforatum in burn / wound treatment.

[0012] As a result, an herbal burn cream that increases the antimicrobial, antifungal, antioxidant and cell proliferation effects by using Hypericum perforatum (St. John's wort) and energized oxygen in burn / wound treatment, and reduces side effects by not using chemical additives, and a production method that enables this cream to be obtained, development is needed.

[0013] Purpose of the Invention

[0014] The present invention relates to an herbal burn cream and a method of manufacturing the herbal burn cream, which fulfills the above-mentioned requirements, eliminates possible disadvantages and provides some additional advantages.

[0015] The main purpose of the herbal burn cream and production method that is the subject of the invention is; The aim is to obtain an herbal burn cream and burn cream production method that increases the antimicrobial, antifungal, antioxidant and cell proliferation effects in burn / wound treatment and reduces side effects by not using chemical additives. Another aim of the invention is to obtain an herbal burn cream and burn cream production method that provides effective healing and disinfection, especially in the treatment of second-degree burns, and increases the effectiveness against bacteria and viruses in sterilization applications.

[0016] Another aim of the invention is to obtain an effective herbal burn cream and burn cream production method that provides a painless treatment for the patient and prevents infection in the burn area, thanks to its anti-inflammatory effect.

[0017] Another aim of the invention is to obtain an efficient herbal burn cream and burn cream production method, which does not need to be supported with external materials such as thickening base, cream or vaseline etc. by increasing its retention rate I effectiveness in oil and its absorption by the skin is increased when applied to the skin in pure form.

[0018] Another aim of the invention is to obtain a reliable herbal burn cream and burn cream production method that does not contain chemical ingredients and external additives and reduces the occurrence of side effects and undesirable situations on the user.

[0019] Another aim of the invention is to obtain a healthy herbal burn cream and burn cream production method that does not form N2O5 derived nitrogen oxide when released into the air, does not react with acid and does not cause stomach damage.

[0020] An alternative aim of the invention is to obtain a natural and functional herbal burn cream and burn cream production method that can be used instead of synthetic products in burn treatment and reduces production costs.

[0021] In order to achieve the above objectives in the most general way, it is necessary to increase the antimicrobial, antifungal, antioxidant, cell proliferation, healing, disinfection and anti-inflammatory effects in burn / wound treatment, to prevent pain effects, infection formation, by not using chemical and thickening additives and / or emulsifier components. It contains herbal burn cream, Hypericum perforatum extract and energized oxygen, which reduce the occurrence of burn effects.

[0022] The herbal burn cream production method that enables the herbal burn cream developed with the present invention to be obtained is by drying the Hypericum perforatum plant and turning it into powder; placing the powdered Hypericum perforatum plant in a solvent and subjecting it to extraction by ultrasonication at a temperature lower than 40°C and obtaining the Hypericum perforatum extract; It includes the steps of obtaining the cream formulation by adding a cream base containing at least 15% tetra oxygen. The structural and characteristic features and all advantages of the invention will be more clearly understood by means of the figures given below and the detailed description written by making references to these figures, and therefore, the evaluation should be made by considering these figures and detailed description.

[0023] Detailed Description of the Invention

[0024] In this detailed description, the preferred embodiments of the inventive herbal burn cream and the method of manufacturing the herbal burn cream are described only for a better understanding of the subject matter and in a non-limiting manner.

[0025] Antimicrobial, antifungal, antioxidant, cell proliferation, healing, disinfectant and anti-inflammatory agents for the treatment of burns / wounds, preferably 2nd herbal burn cream for the treatment of burns / wounds, preferably 2nd degree burns / wounds, which increases antimicrobial, antifungal, antioxidant, cell proliferation, healing, disinfection and anti-inflammatory effects, reduces pain effects, infection formation, side effect formation by not using chemical and thickening additives and / or emulsifier components, Hypericum perforatum (St. John's wort) medicinal oil, preferably in oil form, and energized oxygen (tetra oxygen / O4), preferably in the form of a base suitable for direct mixing with the oil, preferably in the form of petroleum jelly or cream.

[0026] In an exemplary application of the herbal burn cream developed with the present invention, an herbal burn cream is obtained by combining Hypericum perforatum, preferably in oil form, and a cream base containing energized oxygen / tetra oxygen without using any thickeners, emulsifiers and / or chemical materials. Thanks to the Hypericum perforatum in the cream, fibroblastic activity and collagen increase are provided along with antimicrobial and antioxidant effects. The tetra oxygen (C j / energized oxygen base in the cream provides a strong disinfectant effect and provides sterilization against viruses, spore-forming bacteria and antibiotic-resistant bacteria and fungi. In addition, thanks to the higher retention rate of O4 in oil than ozone, a cream form is obtained without containing any thickeners or emulsifiers. However, while the O4 base increases the absorption efficiency of the cream by the skin, it also reduces the possibility of allergic reactions, lung lesions and wound formation in the stomach.

[0027] Table 2. Table of MIC values of plant extracts against pathogenic microorganisms (HP-ETOH: Hypericum perforatum L. ethanol extract) The herbal burn cream production method, which enables the herbal burn cream developed with the present invention to be obtained by drying and powdering the above-ground parts of the Hypericum perforatum plant, preferably weighing 30-50 grams; by placing the powdered Hypericum perforatum plant in a solvent, preferably in a volume of 400-500 milliliters, and ultrasonicating at a temperature lower than 40 °C, preferably 25-37 °C, preferably for 1 hour and repeating the process 3-4 times, subjecting it to the extraction process and obtaining the Hypericum perforatum extract; preferably filtering the obtained extract using coarse filter paper (whatman no:1 type filter paper); preferably repeating the filtration process 6 times; preferably subjecting the extract to evaporation, preferably using a rotary evaporator; concentrating it by drying it in a lyophilizer, preferably at -80 to -120 °C, to completely remove the liquid residues in the extract; preferably storing the concentrated powdered extract at +4 °C; It includes the steps of obtaining the cream formulation by adding a cream base containing at least 15% tetra oxygen, preferably produced using a medical oxygen generator that produces O4 with at least 95% purity (10 L / min).

[0028] In a preferred embodiment of the invention, the said herbal burn cream production method involves placing the powdered Hypericum perforatum plant in a container containing oil, preferably olive oil, preferably in a volume of 400-500 milliliters, and applying day / sunlight for a period of 12 hours a day, preferably for 4 weeks. It includes the step of obtaining medicinal oil by keeping it under water.

[0029] In another preferred embodiment of the invention, the said herbal burn cream production method includes the step of adding at least one aromatic oil, preferably lemon balm oil, lemon oil and / or tea tree oil, as a fragrance agent.

[0030] Table 3. Table of average recovery percentages of in vivo experimental groups on different days (NC: Negative control, PK: Positive Control, TOB: Tetra Oxygenated Base, YK: Burn Cream)

[0031] Thanks to the herbal burn cream and herbal burn cream production method developed with the present invention, the antimicrobial, antifungal, antioxidant, cell proliferation, disinfection and antiinflammatory effects are increased by using Hypericum perforatum (St. John's wort) and energized oxygen in burn / wound treatment. In addition, by not using chemical additives, an efficient, effective and low-cost herbal burn cream and a production method that enables this cream to be obtained, which reduces side effects and damage to organs such as the stomach and lungs, are obtained.

Claims

CLAIMS1. An herbal burn cream which increases antimicrobial, antifungal, antioxidant, cell proliferation, healing, disinfection and anti-inflammatory effects in burn I wound treatment, reduces pain effects, infection formation, side effects by not using chemical and thickening additives and I or emulsifier components, characterized in that;Hypericum perforatum medicinal oil, energized oxygen.

2. An herbal burn cream according to claim 1, wherein the herbal burn cream is suitable for use in the treatment of 2nd degree burns.

3. An herbal burn cream according to claim 1 , wherein the Hypericum perforatum extract is in oil form.

4. An herbal burn cream according to claim 1, wherein the energized oxygen is a base suitable for mixing directly with oil.

5. An herbal burn cream according to claim 1 , wherein the energized oxygen is in the consistency of petroleum jelly or cream.

6. A method of producing an herbal burn cream according to any one of the preceding claims, characterized in that; drying and pulverization of Hypericum perforatum plant; placing the powdered Hypericum perforatum plant in a solvent; obtaining the hypericum perforatum extract by processing the extraction with ultrasonication at a temperature lower than 40 °C; obtaining a cream formulation by adding a cream base containing at least 15% tetra oxygen to the extract.

7. A method according to claim 6, wherein the amount of hypericum perforatum plant in the mixture is 30-50 grams.

8. A method according to claim 6, wherein the Hypericum perforatum plant used in drying and pulverization process is the surface / above the ground parts of the hypericum perforatum plant.

9. A method according to claim 6, wherein the solvent in the volume of 400-500 milliliters.

10. A method according to claim 6, wherein the temperature applied in the ultrasonication process is 25-37 °C.

11. A method according to claim 6, wherein the extraction process is applied for 1 hour and the process is repeated 3-4 times.

12. A method according to claim 6, comprising the further step of filtering the resulting extract, preferably using coarse filter paper.

13. A method according to claim 12, comprising the further step of repeating the filtering process more than once.

14. A method according to claim 6, wherein the extract is subjected to evaporation, preferably by using a rotary evaporator.

15. A method according to claim 6, comprising the further step of concentrating the extract to remove the liquid residues in the extract completely by drying it in a lyophilizer, preferably at -80 to -120 °C.

16. A method according to claim 15, comprising the further step of storing the concentrated extract in the powder form at +4 °C.

17. A method according to claim 6, comprising the further step of using a medical oxygen generator that produces O4 with a purity of at least 95% in the production of tetra oxygencontaining cream base.

18. A method according to claim 6, comprising the further step of adding at least one aromatic oil to the cream formulation, preferably lemon balm oil, lemon oil and / or tea tree oil, as a fragrance agent.

19. A method according to claim 6, comprising the further step of obtaining a medicinal oil by placing the powdered Hypericum perforatum plant in a container containing an oil, preferablyolive oil, preferably 400-500 milliliters in volume, and exposing it to the sunlight for a period of 12 hours a day, preferably for 4 weeks.

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