Disruption of RBR1 expression in plant cells

Abstract

The invention relates to a method for facilitating endosperm development in an angiosperm central cell in absence of karyogamy, or fertilization by a sperm cell. The method according to the invention comprises specifically and selectively inhibiting, or substantially decreasing expression or activity, or lowering the concentration, of RBR1 in the central cell, by cell specific expression of an agent targeting either the expression of the gene encoding RBR1, or by directly or indirectly targeting RBR1 protein through cell-specific expression of an agent leading to degradation of the protein. The invention further relates to a recombinant angiosperm cell having the potential for endosperm development from a central cell in absence of fertilization. A related aspect encompasses a plant comprising such cell, and seed derived therefrom in absence of fertilization.

Description

[0001]DISRUPTION OF RBR1 EXPRESSION IN PLANT CELLS Field The present invention relates to the field of genetic manipulation of plants to facilitate propagation of angiosperm plants without fertilization. Background Seeds are the major products of agriculture and the production of seeds for sowing is a multibillion USD market. Endosperm is one of the major components of seeds and supports the development of the embryo and seedling. Seed production would be tremendously sim- plified, and breeding processes streamlined, by the introduction of apomixis into sexual crops. Apomixis is the asexual reproduction through seeds and entails three developmental differ- ences with respect to sexual reproduction. First, meiosis has to be omitted or circumvented (apmeiosis) leading to the production of unreduced, unrecombined female gametes; second, embryo development from the egg cell has to initiate in the absence of fertilization (par- thenogenesis), leading to the formation of clonal progeny; and third, functional endosperm has to support the formation of the seed, requiring a variety of developmental adaptations, e.g., autonomous endosperm development in the absence of fertilization or changes in gam- etogenesis or fertilization that lead to the formation of balanced, functional endosperm. Based on the above-mentioned state of the art, the objective of the present invention is to provide means and methods to facilitate a crucial component of apomixis, which is endosperm development in a central cell in absence of fertilization. This objective is attained by the subject-matter of the independent claims of the present specification, with further advantageous embodiments described in the dependent claims, examples, figures and general description of this specification. Summary of the Invention A first aspect of the invention relates to a method for facilitating endosperm development in an angiosperm central cell in absence of karyogamy, or fertilization by a sperm cell. The method according to the invention comprises specifically and selectively inhibiting, or substantially decreasing expression or activity, or lowering the concentration, of RBR1 (RETINOBLASTOMA RELATED1) in the central cell, by cell specific expression of an agent targeting either the expression of the gene encoding RBR1, or by directly or indirectly targeting RBR1 protein through cell-specific expression of an agent leading to degradation of the protein. The invention further relates to a recombinant angiosperm cell having the potential for endosperm development from a central cell in absence of fertilization. A related aspect encompasses a plant comprising such cell, and seed derived therefrom in absence of fertilization. Terms and definitions For purposes of interpreting this specification, the following definitions will apply and whenever appropriate, terms used in the singular will also include the plural and vice versa. In the event that any definition set forth below conflicts with any document incorporated herein by reference, the definition set forth shall control. The terms “comprising”, “having”, “containing”, and “including”, and other similar forms, and grammatical equivalents thereof, as used herein, are intended to be equivalent in meaning and to be open-ended in that an item or items following any one of these words is not meant to be an exhaustive listing of such item or items, or meant to be limited to only the listed item or items. For example, an article “comprising” components A, B, and C can consist of (i.e., contain only) components A, B, and C, or can contain not only components A, B, and C but also one or more other components. As such, it is intended and understood that “comprises” and similar forms thereof, and grammatical equivalents thereof, include disclosure of embodiments of “consisting essentially of” or “consisting of.” Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit, unless the context clearly dictates otherwise, between the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within the disclosure, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the disclosure. Reference to “about” a value or parameter herein includes (and describes) variations that are directed to that value or parameter per se. For example, description referring to “about X” includes description of “X.” As used herein, including in the appended claims, the singular forms “a”, “or” and “the” include plural referents unless the context clearly dictates otherwise. "And / or" where used herein is to be taken as specific recitation of each of the two specified features or components with or without the other. Thus, the term "and / or" as used in a phrase such as "A and / or B" herein is intended to include "A and B," "A or B," "A" (alone), and "B" (alone). Likewise, the term "and / or" as used in a phrase such as "A, B, and / or C" is intended to encompass each of the following aspects: A, B, and C; A, B, or C; A or C; A or B; B or C; A and C; A and B; B and C; A (alone); B (alone); and C (alone). Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art (e.g., in cell culture, molecular genetics, nucleic acid chemistry, hybridization techniques and biochemistry, organic synthesis). Standard techniques are used for molecular, genetic, and biochemical methods (see generally, Sambrook et al., Molecular Cloning: A Laboratory Manual, 4th ed. (2012) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. and Ausubel et al., Short Protocols in Molecular Biology (2002) 5th Ed, John Wiley & Sons, Inc.) and chemical methods. Any patent document cited herein shall be deemed incorporated by reference herein in its entirety. General Molecular Biology: Nucleic Acid Sequences, Expression The term gene refers to a polynucleotide containing at least one open reading frame (ORF) that is capable of encoding a particular polypeptide or protein after being transcribed and translated. A polynucleotide sequence can be used to identify larger fragments or full-length coding sequences of the gene with which they are associated. Methods of isolating larger fragment sequences are known to those of skill in the art. The term transgene in the context of the present specification relates to a gene or genetic material that has been transferred from one organism to another. In the present context, the term may also refer to transfer of the natural or physiologically intact variant of a genetic sequence into tissue of a patient where it is missing. It may further refer to transfer of a natural encoded sequence the expression of which is driven by a promoter absent or silenced in the targeted tissue. The term recombinant in the context of the present specification relates to a nucleic acid, which is the product of one or several steps of cloning, restriction and / or ligation and which is different from the naturally occurring nucleic acid. A recombinant virus particle comprises a recombinant nucleic acid. The terms gene expression or expression, or alternatively the term gene product, may refer to either of, or both of, the processes - and products thereof - of generation of nucleic acids (RNA) or the generation of a peptide or polypeptide, also referred to transcription and translation, respectively, or any of the intermediate processes that regulate the processing of genetic information to yield polypeptide products. The term gene expression may also be applied to the transcription and processing of a RNA gene product, for example a regulatory RNA or a structural (e.g. ribosomal) RNA. If an expressed polynucleotide is derived from genomic DNA, expression may include splicing of the mRNA in a eukaryotic cell. Expression may be assayed both on the level of transcription and translation, in other words mRNA and / or protein product. The terms capable of forming a hybrid or hybridizing sequence in the context of the present specification relate to sequences that under the conditions existing within the cytosol of a mammalian cell, are able to bind selectively to their target sequence. Such hybridizing sequences may be contiguously reverse-complimentary to the target sequence, or may comprise gaps, mismatches or additional non-matching nucleotides. The minimal length for a sequence to be capable of forming a hybrid depends on its composition, with C or G nucleotides contributing more to the energy of binding than A or T / U nucleotides, and on the backbone chemistry. The term siRNA (small / short interfering RNA) in the context of the present specification relates to an RNA molecule capable of interfering with the expression (in other words: inhibiting or preventing the expression) of a gene comprising a nucleic acid sequence complementary or hybridizing to the sequence of the siRNA in a process termed RNA interference. The term siRNA is meant to encompass both single stranded siRNA and double stranded siRNA. siRNA is usually characterized by a length of 17-24 nucleotides. Double stranded siRNA can be derived from longer double stranded RNA molecules (dsRNA). According to prevailing theory, the longer dsRNA is cleaved by an endo-ribonuclease (called Dicer) to form double stranded siRNA. In a nucleoprotein complex (called RISC), the double stranded siRNA is unwound to form single stranded siRNA. RNA interference often works via binding of an siRNA molecule to the mRNA molecule having a complementary sequence, resulting in degradation of the mRNA. RNA interference is also possible by binding of an siRNA molecule to an intronic sequence of a pre-mRNA (an immature, non-spliced mRNA) within the nucleus of a cell, resulting in degradation of the pre-mRNA. The term shRNA (small hairpin RNA) in the context of the present specification relates to an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi). The term miRNA (microRNA) in the context of the present specification relates to a small non- coding RNA molecule (containing about 22 nucleotides) that functions in RNA silencing and post-transcriptional regulation of gene expression. Detailed Description of the Invention A first aspect of the invention relates to a method for facilitating endosperm development in an angiosperm central cell in absence of karyogamy, or fertilization by a sperm cell. The method according to the invention comprises specifically and selectively inhibiting, or substantially decreasing expression or activity, or lowering the concentration, of RBR1 (RETINOBLASTOMA RELATED1) in the central cell. As RBR1 is integral to plant cell functionality, the functional knock-out, whether attained on the level of gene expression or on the level of protein degradation or inhibition, needs to be specific to the cell type involved in endosperm formation. The method according to the invention allows to induce endosperm development in the absence of pollination or fertilization, hence karyogamy is not required for the seed to develop. This is important with respect to the engineering of apomixis, whereof autonomous endosperm formation is a crucial element. RBR1 is known under the following synonyms: ATRBR1, RB, RB1, RBR, RETINOBLASTOMA 1, RETINOBLASTOMA PROTEIN, RETINOBLASTOMA-RELATED, retinoblastoma-related 1, RETINOBLASTOMA-RELATED PROTEIN 1. The Ensembl data base entry for the Arabidopsis gene is AT3G12280. The gene tree showing orthologues currently known and depicted in the last figure has the identifier EPlGT00140000002315. It is intended for any protein having an identifiable homology or identifiable as an orthologue to RBR1 as identified by the sequences and identifiers provided herein, to constitute a target according to the invention. Targeting RBR1 expression on the nucleic acid level In particular embodiments, inhibiting or substantially decreasing expression or activity, or lowering the concentration, of RBR1 comprises expression of an agent targeting RBR1, or its orthologue in the specific plant that the plant cell is derived from. In particular embodiments, the agent inhibiting or substantially decreasing expression or activity, or lowering the concentration, of RBR1 is a nucleic acid agent capable of interfering with RBR1 gene expression, particularly on the level of mRNA transcription, transport, maturation or maintenance. In other distinct particular embodiments, the agent inhibiting or substantially decreasing expression or activity, or lowering the concentration, of RBR1 is a polypeptide agent capable of degrading RBR1 protein. Several approaches are known to induce intracellular, cell-type specific abrogation of protein activity. Without being limiting, these include the following: One approach artificial uses a nucleic acid agent expressed specifically in the cell type through cell type specific promoters. Non-limiting examples of such agents are miRNA, shRNA, or siRNAs to induce post-transcriptional gene silencing of RBR1, leading to the inhibition of expression on the mRNA level. In certain embodiments, the agent targeting RBR1 is a ribonucleic acid capable of hybridizing to an mRNA transcribed from the rbr1 gene. In certain particular embodiments, the ribonucleic acid capable of hybridizing to an mRNA transcribed from the rbr1 gene is a miRNA. In certain particular embodiments, the ribonucleic acid capable of hybridizing to an mRNA transcribed from the rbr1 gene is an shRNA. In certain particular embodiments, the ribonucleic acid capable of hybridizing to an mRNA transcribed from the rbr1 gene is a siRNA. Without wanting to be bound by theory, the inventors believe that because RBR1 is essential in all cells, it is not possible to target genomic rbr1 by CRISPR / Cas9 methods. Down- regulation or degradation only in the central cell, however, is possible while the cell awaits fertilization. A system in which dCas9 fused to a repressor domain, e.g. an EAR or SRDX domain is used (Lowder et al., Methods Mol Biol.2017;1629:167-184), and in which dCas9- EAR is targeted to the RBR1 promoter could be used to downregulate RBR1 once the central cell is formed. Targeting RBR1 expression on the protein level Another approach relies on the targeted degradation of RBR1 in the central cell. This can be achieved, for instance, by substituting the native RBR1 with an RBR1 protein that contains a protease cleavage site, such as the TEV protease from Tobacco Etch Virus, and then expressing TEV specifically in the central cell. This leads to RBR1 degradation only in the central cell. See Andrès et al., Methods Mol Biol.2011;775:31-49. A related approach is to use a polypeptide ligand that specifically targets RBR1 to degradation. The principle has been proven to work in plant cells (see Caussinus et al., Nat. Struct. Mol. Biol., 19 (2011), pp.117-121; Ma et al. Nat. Commun., 10 (2019), p.5093; Wang et al., Curr Biol.2021 Nov 8;31(21):4810-4816.e5). Fusion of the target protein to GFP and expressing a GFP-specific antibody fragment, or an antibody-like ligand (exemplified by a nanobody, or a binding protein of the DARPIN class (collectively referred to as BP) as part of a fusion protein with an ubiquitin ligase (BP-UL) induces degradation of the target upon expression of the BP- UL construct. Applied to the invention, substituting the native RBR1 with an RBR1-GFP fusion leads to only the fusion RBR1 protein present in cell. By expressing a nanobody that binds to GFP and is fused to a ubiquitin ligase, the RBR1-GFP protein is specifically targeted for degradation in the central cell only, if the nanobody is expressed only in this cell. This may well be adapted to native RBR1 if an RBR1-specific antibody fragment is ligated to ubiquitin ligase and specifically expressed in the cell. The term antibody-like molecule in the context of the present specification refers to a molecule capable of specific binding to another molecule or target with high affinity / a Kd ≤ 10E-8 mol / l. An antibody-like molecule binds to its target similarly to the specific binding of an antibody. The term antibody-like molecule encompasses a repeat protein, such as a designed ankyrin repeat protein (Molecular Partners, Zürich), an engineered antibody mimetic protein exhibiting highly specific and high-affinity target protein binding (see US2012142611, US2016250341, US2016075767 and US2015368302, all of which are incorporated herein by reference). The term antibody-like molecule further encompasses, but is not limited to, a polypeptide derived from armadillo repeat proteins, a polypeptide derived from leucine-rich repeat proteins and a polypeptide derived from tetratricopeptide repeat proteins. The term antibody-like molecule further encompasses a specifically binding polypeptide derived from a protein A domain, a fibronectin domain FN3, a consensus fibronectin domain, a lipocalin (see Skerra, Biochim. Biophys. Acta 2000, 1482(1-2):337-50), a polypeptide derived from a Zinc finger protein (see Kwan et al. Structure 2003, 11(7):803-813), a Src homology domain 2 (SH2) or Src homology domain 3 (SH3), a PDZ domain, a gamma-crystallin, ubiquitin, a cysteine knot polypeptide or a knottin, cystatin, Sac7d, a triple helix coiled coil (also known as alphabodies), a Kunitz domain or a Kunitz-type protease inhibitor and a carbohydrate binding module 32-2. The term protein A domains derived polypeptide refers to a molecule that is a derivative of protein A and is capable of specifically binding the Fc region and the Fab region of immunoglobulins. The term armadillo repeat protein refers to a polypeptide comprising at least one armadillo repeat, wherein an armadillo repeat is characterized by a pair of alpha helices that form a hairpin structure. The term humanized camelid antibody in the context of the present specification refers to an antibody consisting of only the heavy chain or the variable domain of the heavy chain (VHH domain) and whose amino acid sequence has been modified to increase their similarity to antibodies naturally produced in humans and, thus show a reduced immunogenicity when administered to a human being. A general strategy to humanize camelid antibodies is shown in Vincke et al. “General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold”, J Biol Chem. 2009 Jan 30;284(5):3273-3284, and US2011165621A1. One could also use other, similar approaches that lead to the specific degradation of fusion protein. For the specific degradation, on can use any tag, such as GFP, YFP but also smaller proteins tags against which a nanobody or DARPIN is available that can be expressed in a cell using a specific promoter. In animals, protein degradation is often induced by the phytohormone auxin (Zhang et al., Development 142: 4374–4384.10.1242 / dev.129635; Martinez et al., G3 (Bethesda).2020 Jan 7;10(1):267-280. doi: 10.1534 / g3.119.400781). An auxin-inducible degron (AID) is fused to the target protein and the auxin receptor TIR1, an F-box protein, is expressed in a specific cell type: Upon addition of auxin, the target protein gets degraded allowing on to control time (auxin addition) and space (specific promoter expressing TIR) of target protein degradation. As auxin is a plant hormone and TIR1 expressed in all cells, this is not possible in plants. However, recently an artificial, orthogonal auxin / TIR1 system has been engineered (Uchida et al., Nat Chem Biol. 2018 Mar;14(3):299-305; Yamada et al., Plant Cell Physiol. 2018 Aug 1;59(8):1538-1544) that could be used for the same system as the modified TIR1 is only activated by an artificial auxin that is not present in plant cells. In certain embodiments, the agent targeting RBR1 is a polypeptide agent capable of degrading RBR1. Non-limiting examples of such agent targeting RBR1 are a nanobody (an antibody fragment) or another antibody-like structure linked to an ubiquitin ligase, wherein the antibody- like structure specifically recognizes RBR1. Another example is an endopeptidase that specifically recognizes the RBR1 produced in the cell. In certain embodiments, the angiosperm central cell comprises a recombinant copy of the rbr1 gene encoding a variant of RBR1 instead of (replacing the) the native copy of RBR1, and the polypeptide agent targeting RBR1 is specifically capable of degrading said variant directly. In certain other embodiments, the angiosperm central cell comprises a recombinant copy of the rbr1 gene encoding a variant of RBR1 instead of (replacing the) the native copy of RBR1, and the polypeptide agent targeting RBR1 is specifically capable of inducing degradation of the variant by an intracellular degradation pathway. In particular embodiments, this is achieved by ubiquitinylation and subsequent degradation via the proteasome. In particular embodiments, the polypeptide agent targeting RBR1 is specifically capable of inducing degradation of the variant. Promoters capable of selective expression of the agent in the central cell Expression of said agent targeting RBR1 targeting RBR1 is controlled by a promoter specifically active in said central cell. Many central cell specific promoters are known. A non-limiting example includes the following: pZmEBE (Maize; see Magnard et al., Plant Mol Biol.2003 Dec;53(6):821-36); pMEA (Arabidopsis; Köhler et al., Genes Dev.2003 Jun 15;17(12):1540-53), pDME (Arabidopsis Choi et al., Cell.2002 Jul 12;110(1):33-42), pRALF18 (Arabidopsis Wuest et al.,. Curr Biol.2010 Mar 23;20(6):506-12) pAGL80 (Arabidopsis, Portereiko et al., Plant Cell.2006 Aug;18(8):1862-72) pDD46 (Arabidopsis; Portereiko et al., ibid), pDIA / pAGL61 (Arabidopsis; Bemer et al., Plant Cell.2008 Aug;20(8):2088-101) pFIE (Arabidopsis; Luo et al., Proc Natl Acad Sci U S A.2000 Sep 12;97(19):10637-42) Any scientific publication cited in this specification is incorporated by reference herein. The skilled person is aware of the plenitude of data published on cell-specific expression of the cells in the embryo sac, including the central cell, for Arabidopsis (see Wuest, above, and in addition Susaki et al., PLoS Biol.2021 Mar 26;19(3):e3001123; Song et al., Genome Biol. 2020 Jul 22;21(1):178), and maize (Lê et al., Plant J.2005 Oct;44(1):167-78), and ovules at different stages in rice (Kubo et al. Plant Cell Physiol.2013 May;54(5):750-65), so the skilled artisan will not be required to employ inventive activity to find central cell-specific promoters. For conserved genes, very often the promoters can also be used in other species. As one example, the Arabidopsis egg cell-specific pRKD2 promoter also works in cereals. The present invention, as part of a solution to facilitate the elements of apomixis, is relevant to all crop plants, both mono- and dicots. From a food safety point of view, the most important application will be in the grasses (Poacea) that encompass all important cereal crops. In certain embodiments, the central cell is a cell of a plant comprised in the family Poaceae (grasses). In certain particular embodiments, the central cell is a cell of a plant comprised in a genus comprised in the group consisting of Triticum (wheat). In certain other particular embodiments, the central cell is a cell of a plant comprised in a genus comprised in the group consisting of Hordeum (barley). In certain other particular embodiments, the central cell is a cell of a plant comprised in a genus comprised in the group consisting of Secale (rye). In certain other particular embodiments, the central cell is a cell of a plant comprised in a genus comprised in the group consisting of Zea (maize). In certain other particular embodiments, the central cell is a cell of a plant comprised in a genus comprised in the group consisting of Oryza (rice). In certain other particular embodiments, the central cell is a cell of a plant comprised in a genus comprised in the group consisting of Avena. Another aspect of the invention relates to a recombinant angiosperm cell, comprising a transgene encoding a nucleic acid agent targeting RBR1 under transcriptional control of a promoter sequence specifically operable in an angiosperm central cell. In particular embodiments, the nucleic acid agent is a miRNA targeting the RBR1 encoding mRNA. In particular embodiments, the nucleic acid agent is a siRNA targeting the RBR1 encoding mRNA. In particular embodiments, the nucleic acid agent is an shRNA targeting the RBR1 encoding mRNA. An alternative of this aspect of the invention relates to a recombinant angiosperm cell, that comprises a transgene encoding a polypeptide specifically leading to degradation of RBR1. The transgene is under transcriptional control of a promoter sequence specifically operable in an angiosperm central cell. In particular embodiments, the polypeptide specifically leading to degradation of RBR1 is an antibody-like ligand specifically targeting RBR1, as laid out above in the context of discussion of the method aspect of the invention and any of the embodiments that apply. The cell may comprise a transgene encoding a variant of RBR1 having RBR1 biological activity, the variant being specifically cleavable by a protease, and the polypeptide specifically leading to degradation of RBR1 is a protease capable of specifically cleaving said variant. In particular embodiments of the method or cell aspects of the invention, the promoter sequence is selected from the group consisting of pZmEBE, pMEA, pDME, pRALF18, pAGL80, pDD46, pDIA / pAGL61, pFIE. In particular embodiments of the method or cell aspects of the invention, the cell is a plant cell of a plant comprised in the family Poaceae (grasses) and the RBR1, or its orthologue, or its variant, is the natural RBR1 orthologue of the plant. RBR1 orthologues in many plants are known. The sequences section provides sequences and sequence alingments for a number of commercially important crop plants. Figure 10 provides the gene tree display. Another aspect of the invention relates to a plant comprising an angiosperm cell according to any one of the above embodiments of the cell aspect of the invention. Similarly, a seed of such plant according to the invention, capable of development of endosperm in absence of fertilization, is encompassed. Wherever alternatives for single separable features are laid out herein as “embodiments”, it is to be understood that such alternatives may be combined freely to form discrete embodiments of the invention disclosed herein. The invention is further illustrated by the following examples and figures, from which further embodiments and advantages can be drawn. These examples are meant to illustrate the invention but not to limit its scope. Description of the Figures Fig.1 shows cell cycle dynamic around the moment of fertilization in the EC and CC. a. Circular heat map of the expression level of cell cycle components of Egg Cell (EC), Central Cell (CC), and Pollen (PO). The genes are organized following the cell cycle stages, from G1 to M. A single gene can be represented in more than one stage, accordingly to its function. b-e. Representative images of Propidium Iodide staining of ovules, with a focus on the whole female gamethophyte, at different time points after pollination: 0HAP (b), 6HAP (c), 8HAP (d), after the first endosperm division (e). f. Box plot showing Propidium Iodide staining quantification as ploidy value of Sporophytic cells (Sp), CC, EC, Endosperm (EN), and Zygore (ZY), at different time point after pollination. n.s., not significant; * P value<0.01, ** P value<0.001 accordingly to T-Test. g-h. Representative images of EdU staining of ovules at 0HAP (g) and 8HAP (h). i. Box plot showing EdU signal quantification in Sporophytic cells (Sp), CC, and EC, at different time points after pollination. n.s., not significant; * Pvalue<0.01, ** Pvalue<0.001 accordingly to T-Test. j-m. Confocal analyses of CTD1a-GFP dynamic in the female gametophyte at stage late FG5 (j), stage FG6 (k), and in fertilized ovules (l-m). Cell walls are stained with Propidium Iodide. Fertilized ovules are easily identified as they accumulate Propidium Iodide in the penetrated synergid cell. n. Quantification of the signal intensity of CTD1a-GFP in Sporophytic cells (Sp), CC, EC in virgin and fertilized ovules. Values for each category are normalized on the averaged signal intensity value of ten sporophytic cells surrounding the female gametophyte. n.s., not significant; *** P value<0.0001 accordingly to T-Test. o. Schematic representation summarizing the cell cycle dynamic of EC and CC accordingly to the fertilization event. Virgin and mature ECs are in G2 stage, whereas virgin CCs are in arrested S-phase, whereas fertilized CCs exhibit S-phase reactivation. Scale bar: 20μm. Fig.2 shows RBR1 protein is depleted from the CC nucleus after fertilization through 26S-proteasomal degradation a-b. Clearing analysis of virgin ovules from RBR1 / rbr1-3 plants, showing wild- type female gametophyte (a), and mutant female gametophyte (b) where the CC has undergone division. The CC nucleolus in (a) and the endosperm-like structure nucleoli in (b) have been artificially highlighted in cyan. c. Quantification of the RBR1-YFP signal intensity in Sporophytic cells (Sp) and CC in virgin and fertilized ovules. Values for each category are normalized on the averaged signal intensity value of ten sporophytic cells surrounding the female gametophyte. *** P value<0.0001 accordingly to T-Test. d-g. Representative images of pRBR1::RBR1-YFP ovules at different time points after pollination: 0HAP (d), 8HAP (e-f), and 12HAP (g). Image in (f) has increased contrast to reveal RBR1-YFP signal in the synergid nucleus (white arrowhead). h. Quantification of pRBR1::RBR1-YFP signal in Sporophytic cells (Sp), virgin and fertilized CC, from inflorescence treated with MOCK solution, and the proteasome inhibitors Epoxomycin, Syringolin A, and MG132. Values for each category are normalized on the averaged signal intensity value of ten sporophytic cells surrounding the female gametophyte. n.s., not significant; *** P value<0.0001 accordingly to T-Test. i-k. Clearing analysis of seeds from inflorescences treated with MOCK solution (i), Epoxomycin (j), and Syl-A (k). At the top right corner is the percentage of seeds showing the phenotype. Embryo and endosperm nucleoli are artificially highlighted in yellow and cyan, respectively. Scale bar: 20μm a-b, c-g; 50μm i-k. Fig.3 shows CYCD7;1 messenger and protein have paternal origin and are delivered to the CC at fertilization through karyogamy. a. Graphic representation of the strategy adopted for the LCM transcriptome analysis of CC at different time points: 0HAP, 4HAP, 8HAP, and 12HAP. b. Heat map with cell cycle-related genes that show variation of the expression across the four LCM data points. c-f. In-situ hybridization with YFP antisense probe of pCYCD7;1::CYCD7;1-YFP plants showing no signal in virgin ovules (c), and positive signal in anthers (d), and sperm cell nucleus in mature pollen grains (e) and within growing pollen tubes (f). g-j. In-situ hybridization with YFP antisense probe of wild-type pistils pollinated with pCYCD7:1::CYCD7;1-YFP pollen at 0HAP (g), 4HAP (h-i) and 8HAP (j), labelling the discharged sperm cell nucleus (arrowhead, g), the CC nucleus (i), and CC and EC nucleus and cytosol (j). k-l. Confocal images of pCYCD7;1::YFPYFP-nls virgin (k) and fertilized (l) ovules. m-n. Confocal analysis of pCYCD7;1::CYCD7;1-YFP line in mature pollen grains (m), and pollen tube (n) showing positive signal in the sperm cells nucleus. o-p. Confocal analysis of virgin (o) and pollinated (p) wild-type ovules with pCYCD7;1::CYCD7;1-YFP pollen showing positive signal in the fertilized CC and EC. CC, Central Cell; EC, Egg Cell; PT, Pollen Tube; Syn, Synergid cell; SP, Sperm cell. Scale bar: 10μm e,I,n, otherwise 20μm. Fig.4 shows CYCD7;1 is paternally required for timely RBR1 degradation and cell division in the fertilized CC a-b. Clearing analysis of ovules plants heterozygous for the pMEA::CYCD7;1 construct, showing a wild-type looking ovule with undivided CC (a), and an ovule whit CC proliferation. In the top right corner is the percentage of ovules showing the phenotype. c-d. Clearing analysis of ovules of heterozygous plants expressing the pRPL18::CYCD7;1 and pRPL18::CYCD7;1mutconstructs, showing a ovule whit CC proliferation (c) and wild-type looking ovules (d). In the top right corner is the percentage of ovules showing the phenotype. e. Quantification of pRBR1::RBR1-YFP signal in pMEA::CYCD7;1 / + background at 5 days post-emasculation, in undivided CC and endosperm-like structure nuclei. For each ovules showing CC proliferation, 4 nuclei have been selected *** Pvalue <0.0001 accordingly to T-Test. f. Example image of pRBR1::RBR1-YFP signal in pMEA::CYCD7;1 / + background in a ovule that showed CC proliferation in absence of fertilization. g-j. Clearing analysis of fertilized ovules showing four endosperm stages: fertilized CC (d), two (e), four (f) and 8 (g) endosperm nuclei. k. Clearing analysis of a wild-type ovule fertilized with cycd4D pollen at 12HAP, showing an undivided CC in proximity of the elongating zygote. l. Classification of fertilized ovules (in percentage) based on the number of endosperm nuclei at a certain time point (8, 10, 12, 16, 24 HAP), in crosses between WT x WT, WT x cycd7;1-1, and WT x cyc4D. Sample size is indicated at the right side of each bar. Figure legend at the bottom of the page. m. Classification of fertilized ovules (in percentage) based on the number of endosperm nuclei at 24 HAP, in reciprocal crosses between WT, cycd7;1-1, and cyc4D individuals. Sample size is indicated at the right side of each bar. n. Confocal analysis of pCYCD7;1::YFPYFPnls line at 16HAP in reciprocal crosses with WT plants showing activation of the maternal CYCD7;1 copy. o. Box plot showing the quantification of pRBR1::RBR1-YFP signal in virgin CC, and in RBR-YFP pistils pollinated with wild-type, cycd7;1 or cyc4D pollen. n.s., not significant; ***Pvalue<0.0001 accordingly to T-Test. p. Quantification of ploidy value through Propidium Iodide staining of CC and EC at 8HAP of wild-type pistils pollinated with wild-type, cycd7;1 or cyc4D pollen. **Pvalue<0.001 accordingly to T-Test. o. Graphic representation summarizing the final model proposed in this work: delivery of paternally originated CYCD7;1 messenger and protein at fertilization triggers completion of the S-phase in the CC through RBR1 depletion, thereby kicking cell cycle progression to allow timely division. Scale bar: 20μm Fig.5 shows (A) WT embryo sac (B) Ovule containing an embryo sac with over- proliferating nuclei at the micropylar pole observed in a transgenic line expressing the amiR against RBR1 under the control of the pAtRKD1 promoter. (C), (D) Ovules with over-proliferating nuclei in transgenic lines in which expression of the amiR against RBR1 was controlled by the pAtEASE promoter. CC- central cell; EC-egg cell; SC-synergid cells; arrows indicate nuclei. Fig.6 shows a. Ploidy of CC (green bars) and EC (yellow bars) at different time points. Fig.7 shows a. Pairwise Pearson correlation coefficients and hierarchical clustering of samples after read count normalization. b. PCA of all samples using the normalized read counts. The first two components explain 86.1 % of the variation in the data set. c. Representative pairwise scatter plot between two replicates. d. Heatmap with differentially expressed genes, a random subset of 500 genes is shown. Fig.8 shows a-b. Confocal images of crosses between pRBR1::mCherry-RBR1 with pCYCD7;1::CYCD7;1-YFP showing presence of RBR1-mCherry and CYCD7;1- YFP in the CC (a), and absence of RBR1-mCherry with CYCD7;1-YFP presence (b). c. Box plot showing the quantification of pRBR1::mCherry-RBR1 with pCYCD7;1::CYCD7;1-YFP signal in fertilized ovules. d. Schematic representation of the CRISPR / Cas9 allele created for CYCD7;1., wherein the WT-Sequence corresponds to 5’-attataggttccatgcgtttcaatggctaa-3’ (SEQ ID NO: 1) and the Mutant corresponds to 5’-attataggttccatgcttcaatggctaa-3’ (SEQ ID NO: 2) e. Classification of fertilized ovules (in percentage) based on the number of endosperm nuclei at a certain time point (8, 10, 12, 16, 24 HAP), in crosses between WT x WT, WT x cycd7;1-1, WT x cyc4D, WT x cycd7;1-2, WT x cycd7;1-3, and WT x cycd7;1-CRISPR. Sample size is indicated at the top of each bar. Fig.9 shows a. Chart of virgin and fertilized ovule percentage for assessing pollen tube velocity from various mutant pollen compared to wild-type. b-c. Schematic representation of the CRISPR / Cas9 allele created for CYCD7;1 and CYCD3;2 to create the quadruple cycd3;1 cycd3;2 cycd3;3 cycd7;1 mutant (cyc4D), wherein the sequences depicted correspond to 5’-gatgggctctattgcgaggaagaa-3’ (SEQ ID NO: 3), 5’-gatgggctctatgttaatcagtaacgaggaagaa-3’ (SEQ ID NO: 4), 5’- cgaattggatggtggaactagt-3’ (SEQ ID NO: 5), 5’-cgaattggattggtggaactagt-3’ (SEQ ID NO: 6) from top to bottom. Fig.10 shows the gene tree for RBR1 (EPlGT00140000002315). Examples Example 1: Sexual reproduction entails the specification of the germline and eventually the formation the male and female gametes that fuse during fertilization. In flowering plants (angiosperms), fertililzation is unique as it involves two pairs of gametes: two sperm cells (SP) are delivered by the pollen tube to the female gametophyte where one each fuses the egg (EC) and the central cell (CC), respectively, in a process known as double fertilization. The zygote originates from the fertilization of the EC, whereas fusion of the second SC with the homo-diploid CC gives rise to the endosperm, a triploid tissue with placenta-like function that sustains embryonic growth. One of the most evident consequences of fertilization is the initiation of mitotic divisions of the newly formed cells. However, in case of inequity between the two parental genomes, cell division arrests soon after fertilization, leading to the abortion of the progeny. To ensure normal development, the cell cycle of paternal and maternal gametes arrest such that they do not divide in the absence of fertilization and are synchronized when nuclear fusion (karyogamy) occurs. For instance, mammalian sperm and egg cells await in G1 and in M phase of meiosis II, respectively. In plants, depending on the species, sperm cells are arrested in G1 or G2, whereas the cell cycle stage at which the female gametes attain quiescence is still under debate. More than a hundred factors govern cell cycle progression and arrest, with their deregulation often leading to reduced fertility because of aberrant gametogenesis and / or embryogenesis. Interestingly in Arabidopsis, mutations in some essential cell cycle genes affect only one of the gametes, with some striking examples regarding the CC where opposite phenotypes, such as proliferation in absence of fertilization or lack of division upon karyogamy, have been observed. These findings have fuelled the hypothesis that the CC has a mechanism to prevent cell division, which is then lifted by a fertilization-dependent signal. Albeit attractive, the molecular players behind this hypothesis are yet to be identified. In order to investigate cell cycle dynamics around fertilization, the inventors first assessed the stage at which mature female gametes arrest. Analysis of the expression levels of components of the cell cycle machinery using available transcriptomic datasets (Schmid, M. W. et al. PLoS ONE 7, e29685 (2012); Schmidt, A. et al. PLoS Biol.9, e1001155 (2011); Loraine, A. E. et al., Plant Physiol.162, 1092–1109 (2013)) for the EC, CC, and pollen (PO) suggested that the CC expresses high levels of virtually all factors involved and necessary for DNA replication during S-phase, while this is not the case for EC and PO (Fig. 1a). To explore this aspect at the cellular level, the inventors quantified the DNA content in the EC and CC using ropidium iodide, which revealed that the unfertilized (virgin) EC and CC have a ploidy level of 1n / 2c and about 2n / 3c, respectively (Fig.1b-f), with n indicating chromosome copies (1n = haploid, 2n = diploid), and c the number of sister chromatids. For example, 1n / 2c indicates a cell with haploid number of chromosomes (1n), where the DNA has been replicated but the two sister chromatids are not yet separated (2c). From the inventors’ analysis, the inventors conclude that the EC reaches the G2 stage of the cell cycle right before fertilization, whereas the CC is either progressing through or arrested in S-phase. To distinguish between these two possibilities, the inventors incubated inflorescences with the nucleotide analogue 5-Ethynyl-2′-deoxyUridine (EdU), which allows the visualization of DNA synthesis in situ. The virgin CC did not incorporate EdU even under prolonged incubation time (Fig. 1g,i), indicating the absence of DNA synthesis. The inventors confirmed this observation by monitoring the expression of the replication licensing factor CTD1a-GFP, whose degradation is promoted by entry and progression through S-phase. CTD1a-GFP accumulated in the virgin CC (Fig. 1j-l,n), being detectable already in the two polar nuclei at the female gametophyte before they fuse (Fig.1j- k). Altogether, these data suggest that the virgin CC is arrested in S-phase at maturity. After fertilization, the inventors observed remarkable changes in the CC. Firstly, the DNA level increased from 2n / 3c to about 3n / 5c, indicating successful fertilization (Fig.1f and Fig.6a) and supporting previous observations that the sperm cell has a DNA amount of 1n / 2c. At about eight hours after pollination (HAP), the fertilized CC reached a ploidy level of 3n / 6c (Fig.1f and Fig. 6a). Nuclear fusion between SP and EC or CC happens independently of each other. Indeed, the inventors detected ovules where the EC showed ploidy of 2n / 4c, while the CC still exhibited a ploidy level of 2n / 3c (Fig.6a), with a visible sperm nucleus in the proximity. This scenario suggests that sperm delivery within the female gametophyte is not enough to trigger cell cycle reactivation in the CC but, instead, that karyogamy is required, as an increase of the ploidy level in the CC occurs only after it reached the minimum value of 3n / 5c. In addition to the increment of ploidy occurring after karyogamy, the inventors also observed faint but detectable EdU staining in the CC (Fig.1h-i), and depletion of CTD1a-GFP exclusively form the CC (Fig. 1m-o), with both events taking place only in fertilized ovules. The EC behaved very differently: following fertilization, its ploidy sharply increased from 1n / 2c to 2n / 4c (Fig.1f and Fig.6a), without detectable EdU incorporation (Fig.1i) and persisting CTD1a-GFP signal (Fig.1n). Altogether, these observations confirm that the mature EC and SP are arrested at the G2 stage of the cell cycle, and revealed that the CC is arrested during S-phase, which is completed only after karyogamy (Fig.1o). The inventors next sought to investigate the mechanism underlying karyogamy-dependent S- phase reactivation in the CC by focusing on the core cell cycle regulator RETINOBLASTOMA RELATED1 (RBR1). RBR1 is a potent inhibitor of entry into and progression through S-phase, and rbr1 mutant CCs exhibit uncontrolled divisions to produce an endosperm-like structure (Fig.2a-b), suggesting that RBR1 might be required for S-phase arrest in the CC. Therefore, the inventors investigated RBR1 protein dynamics in the CC around fertilization. Live imaging analysis of pRBR1::RBR1-YFP in unfertilized ovules confirmed the previously characterized accumulation of RBR1 in the virgin CC (Fig.2c-d). However, around 7-8HAP, pRBR1::RBR1- YFP signal in the CC became progressively weaker and finally undetectable, before reappearing in the first two endosperm nuclei (first CC division; Fig. 2c-g). To investigate whether RBR1 degradation in the CC is mediated the 26S proteasome, as occurs in animals and in other plant tissues, the inventors treated pistils with the selective 26S proteasome inhibitors Epoxomycin, Syringolin-A (SylA), and MG-132, and repeated the live imaging analysis of pRBR1::RBR1-YFP around the moment of fertilization (Fig.2h). Epoxomycin and SylA induced persistence of the pRBR1::RBR1-YFP signal in the fertilized CC (Fig. 2h) in comparison to MOCK-treated pistils (Fig. 2h), thereby preventing - or slowing down - pRBR1::RBR1-YFP degradation in the CC nucleus. Although known to be an inhibitor of RBR1 degradation, MG-132 did not have significant effects on pRBR1::RBR1-YFP stability in the CC under the inventors’ experimental conditions (Fig. 2h). A possible explanation lies in the chemical properties of the inhibitors: MG-132 is a reversible inhibitor that is highly unstable in aqueous solutions, whereas Epoxomycin and Syl-A are high-affinity irreversible, water-stable inhibitors. The inventors repeated Epoxomycin and Syl-A treatments and left the pollinated pistils to develop for a further four days. Remarkably, a single treatment was sufficient to induce the development of seeds where the normal-looking globular stage embryos were surrounded by a few, massively enlarged endosperm nuclei (Fig.2i-k). Similar endosperm phenotypes also develop in plants lacking subunits of the DNA replication machinery, such as ORIGIN OF REPLICATION COMPLEX2 (ORC2), MICROSOMAL MAINTENANCE2 (MCM2), MCM7 / PRL, and CULLIN4 (CUL4), an E3 ubiquitin-ligase involved in protein degradation through the 26S proteasome. Altogether, these data suggest that in the virgin CC, the presence of RBR1 leads to an arrest of the cell cycle in S-phase. Moreover, removal of RBR1 through the 26S proteasome-mediated degradation pathway is a pre-requisite for S-phase completion and to ultimately ensure synchronized maternal and paternal genomes to initiate endosperm development. In order to understand how RBR1 degradation is specifically achieved at karyogamy in the CC, the inventors performed transcriptome analyses of CC at different time points isolated by Laser-Assisted Microdissection (LAM) (Fig.3a, Extended Data Table 1 and Fig.7a-d). Stages encompassed 0HAP (virgin CC), 4HAP (fertilization occurred in about 50% of the CC), 8HAP (all CC are fertilized), and 12HAP (around the first endosperm division). The inventors’ goal was to identify transcripts whose expression peaked around 8HAP, the time point at which RBR1 degradation occurs in the CC, to differentiate between two possible scenarios: either their transcription occurs de novo in a fertilization-dependent manner, or they are of paternal origin and were delivered to the CC upon gamete fusion. Since phosphorylation-dependent RBR1 degradation occurs by a cell-cycle regulated mechanism, the inventors focused on a list of cell cycle components whose expression changed across the four data-points (Fig.3b). One candidate belonging to the Cyclin D-type family, namely CYCD7;1, captured the inventors’ attention for several reasons: (i) D-type cyclins mediate entry and progression through S- phase; (ii) CYCD7;1 interacts with RBR1 and promotes its degradation; (iii) CYCD7;1 transcript was absent in virgin CC, sharply increased at 8HAP (Fig.3b), and was the only D-type cyclin with high expression in pollen, and (iv) ectopic expression of CYCD7;1 in ovules induces CC proliferation in absence of fertilization. Baed on these observations, an attractive hypoothesis is that CYCD7;1 is a paternal messenger loaded into the SP that is delivered to the CC by means of fertilization, thus provoking RBR1 degradation and triggering S-phase completion. To confirm this hypothesis, the inventors first investigated the origin of the CYCD7;1 mRNA by in-situ hybridization of wild-type pistils fertilized with pollen from the pCYCD7:1:;CYCD7;1-YFP line, using an antisense probe specific to YFP (Fig. 3c-j). A signal was observed in the pCYCD7:1:;CYCD7;1-YFP line during pollen development (Fig.3d) and in the SP nucleus of both mature pollen grains and elongating pollen tubes (Fig.3e-f). A strong, punctuate signal was also observed in wild-type ovules fertilized with pCYCD7:1:;CYCD7;1-YFP pollen at about 4HAP, labelling the discharged SP nucleus (Fig.3h). After that, the signal was detected in the CC, initially with a precise nuclear confinement (4HAP, Fig.3i), and later also in the CC (and EC) cytoplasm (Fig.3j). To further corroborate that de novo CYCD7;1 transcription does not occur in the CC, neither from the maternal nor the paternal allele, the inventors analysed the expression pattern of the transcriptional fusion pCYCD7;1::YFP-YFPnls. The YFP signal was detected sporadically in the synergis cells of unfertilized ovules (Fig.3k) but not in the virgin or fertilized CC and EC (Fig.3l). Previous work demonstrated that the CYCD7;1 protein expression is restricted to the stomatal lineage and mature pollen. Therefore, the inventors wondered whether, in addition to the mRNA, also the CYCD7;1 protein could be stored in the sperm nucleus and delivered to the female gametophyte. Analysis of pCYCD7:1:;CYCD7;1-YFP confirmed this hypothesis: CYCD7;1-YFP-expressing SP nuclei were visible in mature pollen grains (Fig.3m), SP nuclei inside growing pollen tubes (Fig. 3n), and the fertilized CC and EC nuclei (Fig. 3o-p). Altogether, these results demonstrate that CYCD7;1 is a paternally derived factor, with its transcripts and protein being delivered directly to the nuclei of the female gametes. The inventors propose that this specific, restricted nuclear localization of mRNA and protein secures that CYCD7;1 is active only upon successful karyogamy, and not simply after SP delivery. The inventors next questioned whether CYCD7;1 promotes RBR1 degradation in the CC. Pollination of pRBR1::mCherry-RBR1 pistils with pCYCD7;1::CYCD7;1-YFP pollen showed that RBR1 depletion in the CC indeed occurs after CYCD7;1 delivery (Fig.8a-c). Moreover, ectopic expression of CYCD7;1 in the CC under a CC-specific (pMEA::CYCD7;1; n=28 / 30 independent transformants, Fig. 4a-b) or a ubiquitously expressed promoter (pRPL18::CYCD7;1, n=18 / 26 independent transformants, Fig.4c), respectively, induced the development of endosperm-like structure in virgin ovules. The nuclei showed a very weak or undetectable pRBR1::RBR1-YFP signal (Fig.4e-f), indicating that CYCD7;1 alone is sufficient to induce RBR1 degradation in the CC and, consequently, to stimulate cell cycle progression. Because the interaction between RBR1 and CYCD7;1 is mediated by the Leu-x-Cys-x-Glu (LxCxE) motif, the inventors ectopically expressed a CYCD7;1 LxCxE mutated variant (pRPL18::CYCD7;1mut). Contrarily to the wild-type version, CYCD7;1mutwas incapable of inducing CC proliferation in virgin ovules (n=58; 0% of endosperm-like structures detected, Fig.4d), suggesting that the interaction of CYCD7;1 with RBR1 is required to mediate S-phase progression in the CC upon fertilization. To investigate whether CYCD7;1 exerts a paternal control over cell division in the fertilized CC, the inventors characterized CYCD7;1 T-DNA insertional mutants (cycd7;1-1 (Weimer, A. K. et al. Dev. Camb. Engl. 145, dev160671 (2018)), cycd7;1-2 (Weimer, A. K. et al. ibid), and cycd7;1-3) and new mutant alleles created by CRISPR-Cas9 technology (cycd7;1CRISPR, Fig. 8d). The inventors focused on CC division by scoring the percentage of fertilized ovules with one undivided CC (Fig.4g), or two, four, or eight endosperm nuclei (Fig.4h-j). In reciprocal crosses between wild-type pistils and cycd7;1 mutant pollen, the inventors observed a significant delay of the first CC division (Fig.4l and Fig.8e). Wild-type x wild-type crosses at 10HAP showed two endosperm nuclei in about 30% of the fertilized ovules (Fig.4h,l and Fig. 8h), while in seeds originating from crosses between wild-type x cycd7;1 mutants, less than 5% had two endosperm nuclei (Fig.4l and Fig.8e). The inventors confirmed that the delay in endosperm initiation was not caused by delayed fertilization, as wild-type and cycd7;1 mutants pollen tubes have comparable growth rates (Fig. 9a). Because seeds receiving a cycd7;1 mutant allele started to recover around 16HAP (Fig. 4i), the inventors investigated whether other D-type cyclins could compensate for the absence of CYCD7;1 and, therefore, created the quadruple mutant cycd3;1 cycd3;2 cycd3;3 cycd7;1 (referred to as cyc4D; Fig.9b-c). Wild- type pistils pollinated with cyc4D pollen showed an even longer delay in the first endosperm division (99% of ovules at 10HAP still had an undivided CC; Fig.4h-i). Embryo development was not affected and the inventors detected several fertilized ovules with an undivided CC nucleus next to an elongating zygote visible (Fig.4k). Remarkably, the delay in initiation of the first endosperm division was further exacerbated when cyc4D pistils were pollinated with cyc4D pollen (Figure 4m), suggesting that the fertilized CC becomes transcriptionally active within few hours upon fertilization with active transcription of cell cycle components. In support of this hypothesis, the inventors detected a pCYCD7:1::YFP-YFPnls signal from the maternal allele in 4-nuclear endosperm (around 20HAP, Fig. 4n), suggesting activation of CYCD7;1 transcription. CYCD7;1 is also among the cell cycle components that show transcriptional activation from the maternal genome soon after fertilization in the zygote. Finally, the inventors investigated RBR1 protein dynamics and the ploidy level of the fertilized CC when using wild-type, cycd7;1, or cyc4D plants as pollen donors. The inventors detected depletion of pRBR1::RBR1-YFP in the fertilized CC at around 8HAP only when using wild-type pollen, while ovules that received cycd7;1 or cycd4D mutant pollen showed persistence of the RBR1-YFP signal (Fig.4o). Consistently, the stability of RBR1-YFP protein is accompanied by a lack of S-phase progression: CCs of ovules that received cycd7;1 or cyc4D pollen failed to precisely reactivate S-phase after fertilization. In these cases, CC ploidy remained at a level of about 3n / 5c at 8HAP, a time when all CCs that were fertilized by wild-type pollen had reached the 3n / 6c value (Fig. 4p). Therefore, in absence of paternal delivery of CYCD7;1, RBR1 depletion fails to occur in the CC at the time of fertilization, thus delaying S-phase reactivation. Example 2: Cell type-specific downregulation of RBR1 in Arabidopsis thaliana induces cell division To test whether a reduced level of RBR1 can indeed induce cell division of quiescent cells in the female gametophyte (embryo sac), the inventors specifically downregulated RBR1 in the Arabidopsis female gametophyte (embryo sac). In order to spatio-temporarily restrict the silencing of RBR1, the inventors chose an artificial microRNA (amiR)-based gene-silencing approach (http: / / wmd3.weigelworld.org / cgi-bin / webapp.cgi). Expression of the amiRs directed against RBR1 was restricted to the egg apparatus, encompassing the egg and synergid cells, by using the egg apparatus-specific enhancer (pAtEASE), or to the egg cell only by using and pAtRKD1 promoter, respectively. This experiment was performed in the background of the tightly regulated, conditionally male-sterile mutant dde2-2, preventing self-fertilization. Microscopical analysis revealed over-proliferatino of nuclei at the micropylar pole of the embryo sac in some transgenic lines (Fig.5). Although this analysis did not reveal structures that resembled embryos in either the lines expression amiRs against RBR1 under the pAtEASE or the pAtRKD1 promoter, it demonstrated that down-regulation of RBR1 can induce cell division. Moreover, embryo sacs were distinctly enlarged and disorganised. This phenotype was rare, occurring in about 1-2% of the ovules, but it was consistently observed in 9 out of 18 transgenic lines carrying an amiR targeting RBR1 driven by the pAtESAE promoter, and 3 out of 18 lines in which the amiR against RBR1 was controlled by the pAtRKD1 promoter. Furthermore, microscopic observations revealed that these multiple additional nuclei observed in mutants carrying amiRs against RBR1 were located in the region of the egg apparatus, coinciding with the restricted expression of the amiR. In some ovules, the inventors could still observe a normal central cell nucleus, suggest that in this region of the embryo sac, cell division was not affected. Since both promoters used in the experiment are not active in this cell, this observation demonstrates that a spatio-temporally restricted down-regulation of RBR1 in the egg cell or egg apparatus can led to local effects in cell proliferation. This finding is consistent with the activity of promoters that were used to mis-express microRNA. Furthermore, the observed phenotype was more frequent in transgenic lines, in which the amiR was driven by the pAtEASE promoter. In contrast to lines, in which the amiR against RBR1 was expressed only in the egg cell from the pAtRKD1 promoter, more nuclei were observed in the egg apparatus region. This observation may indicated that, in addition to the egg cell, also the synergid cells may have undergone additional cell divisions, since the pAtEASE promoter is active also in these two cells. In summary, these results show that a spatio-temporally restricted downregulationo of RBR1 can induce cell division. That the frequency of this phenotype is low can be expected as the quiescent egg cell is arrested in the G2 phase of the cell cycle when RBR1 is not expressed. However, embryo sac development is not completely synchronous in all ovules and in the few embryo sacs where the egg cell and possibly synergids have not yet reached the quiescent state, downregulation of RBR1 is expected to have an effect. Nevertheless, these experiments show that a locally restricted manipulation of RBR1 is possible and can induce cell division. This is expected to be very efficient in the central cell, which is arrested in S-phase awaiting fertilization. There, degradation of RBR1 is crucial for cell cycle progression. Example 3: Material and Methods Plant material and growth conditions Seeds were sown on half-strength MS media (1 / 2 MS salt base [Carolina Biologicals, USA], 1% sucrose, 0.05%MES, 0.8% Phytoagar [Duchefa], pH>5.7 with KOH), stratified for 3-4 days at 4˚C in the dark, and then moved to long-day conditions (8h dark at 18˚C, 16h light at 22˚C, 70% humidity). When showing four true leaves, seedlings were transplanted to soil and grown under long-day conditions in a walk-in growth chamber (8h dark, 16h light, 22˚C, 70% humidity). Marker lines used in this study are: pRBR1::RBR1-Venus (Cruz-Ramírez, A. et al. Cell 150, 1002–1015, 2012), pCYCD7;1::CYCD7;1-YFP (Adrian, J. et al. Dev. Cell 33, 107– 118, 2015), pCYCD7;1::YFP-YFPnls (Weimer, A. K. et al. ibid), pRBR1::mCherry-RBR1 (Biedermann, S. et al., EMBO J. 36, 1279–1297, 2017). Mutant alleles for CYCD7;1 were obtained from NASC and INRA / Versailles (Versailles, France) collections: cycd7;1-1 (FLAG_369E02 (Weimer, A. K. et al. ibid)), cycd7;1-2 (FLAG_498H08 (Weimer, A. K. et al. ibid)), cycd7;1-3 (GABI_496G06). Mutants for cycd3;1 (GABI GABI_529C07) and cyd3;3 (GK- 169B01) were obtained from NASC. When performing reciprocal crosses, flowers at stage 11 of development were emasculated, and left 24h to allow complete maturation of the ovules. Pollination has been done always around 8am, and samples collected, fixed, or imaged at the desired time points in the afternoon / evening. Exception was for the 16HAP time point, were pollination has been performed around 17.00, to avoid collection of material during the night. Vectors For construction of pRPL18::CYCD7;1, the pRPL18 and the CYCD7;1 genomic sequence were amplified from Col-0 genomic DNA using respectively the couple of primers 169-170 and 528-525. The PCR fragments were cloned into the miniT vector (NEB), and assembled together in a Golden Gate reaction together with the terminator tHSP18.2 (Addgene GB0035) and the destination vector p140 using the enzyme BsaI. The destination vector p140 is a modified version of the Golden Gate low copy binary vector pAGM53451 (Grützner, R. et al. Plant Commun.2, 100135, 2021). For the inventors’ purpose, the inventors inserted in the pAGM53451 vector the RedSeed selector marker at position three, whereas at position two the inventors inserted the lacZ gene adapted to be an acceptor for Level 1 Golden Gate modules. For the pRPL18::CYCD7;1mut, the inventors mutated the LxCxE motif to XxXxX, accordingly to Weimer et al., 2018, using the same strategy as above by amplifying the CYCD7;1 gene with primer 577-525, where the former contained the mutations. For the pMEA::CYCD7;1, a 4.5Kb fragment of the MEA promoter was amplified with primers M1 and M2 and cloned in miniT plasmid. The construct was assembled as describe before using the Golden Gate system together with and the miniT- CYCD7;1, the terminator tHSP18.2, and the backbone p140. For pRPL18::CTD1a-GFP , the CTD1a gene was amplified in two parts from Col-0 genomic DNA using the primers 351-345 and 346-352, cloned in the miniT plasmid (NEB), and the assemble with the Golden gate system using the modules for GFP, terminator tHSP18, and the destination vector p140. Imaging and image analysis All imaging of pRBR1::RBR1-YFP line has been performed using a Leica TCS SP8 Multiphoton microscope, equipped with RLD detectors, and in photon counting mode. Z-stacks that include the entire embryo sac have been acquired. Then, the focal planes encompassing CC, EC or sporophytic cells have been merged in a single plane image using the Sum command. For each ovule, 10 sporophytic cells of the inner integument surrounding the embryo sac have been measured to normalize the signal intensity of the CC and EC. Nuclei have been manually identified. Proteasome inhibitor treatment Inflorescences were cut leaving 1.5cm stem, and inserted in a 2ml cryotube filled with MS solid media (1 / 2 MS salt base [Carolina Biologicals, USA], 1% sucrose, 0.05%MES, 0.8% Phytoagar [Duchefa], pH>5.7 with KOH), supplemented with the desired inhibitor (Epoxomycin SIGMA 5uM, Syl-A , and MG-132 SIGMA) incubated in a Percival growth cabinet for the desired time. Alternatively, inflorescences were dipped in a solution made of water, inhibitor at the desired concentration, 0.02% Tween-20. Both methods gave similar results. EdU staining Inflorescences were cut leaving a 1.5cm stem from the apex, and developing siliques and fertilized flowers were removed with a vertical movement in order to peel away strips of epidermis. The inventors observed that such scarification of the stem was necessary to ensure efficient EdU uptake. Inflorescences were then placed in a 1.5ml eppendorf tube filled with MS liquid media (1 / 2 MS salt base [Carolina Biologicals, USA], 1% sucrose, 0.05%MES, pH>5.7 with KOH). The inventors created a small hole in the tube lid where the inventors inserted the inflorescence, so to avoid evaporation of the media. After that, the inventors applied to the samples a gentle vacuum (2min with the vacuum valve slightly open). Tiny air bubbles should be visible on the submerged part of the stem. This step is fundamental to ensure efficient EdU uptake. The samples were then placed in a Percival growth cabinet and left overnight to recover. The morning after, the inflorescences were moved to a new 1.5ml tube filled with EdU- MS media (1 / 2 MS salt base [Carolina Biologicals, USA], 1% sucrose, 0.05%MES, pH>5.7 with KOH 200ml of MS 2% sucrose with 5mgr EdU), and incubated for the desired time in the Percival cabinet. Mature pistils were manually pollinated early morning for the time-course experiment. At the desired time, carpels were collected, sliced open along the replum, and incubated in fixative (Paraformaldehyde 4% in PBS1X pH7.4) for 30min at 4˚C applying an initial 2 min vacuum, and then washed twice with PBS-Tween0.1%. Where required, samples were kept in PBS1X over / night at 4˚C. Samples were then incubated in Digestion mix (cellulase 0.5%, pectolyase 0.5%, driselase 1%), for 1.5 hours at 37˚C, and washed three times with PBS-Tween0.1%. Samples were then incubate for 1.5 hours 4˚C in PBS1X-Tween2%, and then washed three times with water. The samples were then gently moved in a new clean tube filled with 100μl of Copper solution (Copper sulfate 6mM, 3μl of TEG-Azide CATXXX, 2% Tween- 20).100μl of Sodium Ascorbate 30mM were squirted over by placing the eppendorf tube on a vortex at low speed. Samples were then incubated in the completed Click-reaction for 1h at RT in the dark. After that, samples were rinsed several times with abundant water (at least 10 washes of 10 min each, until the samples do not leak yellow anymore), mounted in anti-fade vectashield, and imaged at a Leica SP5 following settings for AlexaFluor 488. Propidium Iodide staining Propidium Iodide staining for ploidy quantification has been performed according to She et al., JoVE J. Vis. Exp. e51530 (2014). Briefly, pistils were gently sliced along the replum and incubate in BVO buffer (2mM EGTA pH7.5, 1% formaldeyde, 10% DMSO, 0.1% Tween-20) for 30 min at RT on an orbital shaker. After that, samples were washed once with PBS1X- Tween0.1%, incubate 5 min in methanol, 5 min in ethanol, and stored at -20˚C or processed immediately. Samples were then incubate in ethanol:xylene 1:1 mixture for 30min at RT, rinsed with ethanol for 5 min, and then with methanol for 5 min. Samples were then fixed in Fixative 2 (methanol:PBS-Tween0.1%1:1, 2.5% formaldehyde), for 15min at RT, and washed twice with PBS-Tween0.1%. Samples were then incubated in the Digestion mix (cellulase 0.5%, pectolyase 0.5%, driselase 1%), for 2 hours at 37˚C. The inventors recommend to optimized the digestion time for each new batch of digestion mix. After that, samples were washed twice with PBS- Tween0.1%, and incubated in PBS1X-Tween1%supplemented with RNase (Qiagen) at a final concentration of 100ugr / ml for 1.5hours at 37’C. Samples were then washed twice with PBS1X-Tween0.1%, and fixed in Fixative 3 (2.5% formaldehyde, PBS1X-Tween0.1%), for 20min at RT, and washed twice with PBS-Tween0.1%. The samples were then incubated 2 hours at 4˚C in PBS1X-Tween2%, washed twice with PBS-Tween0.1%, and incubated with Propodium Iodide 10ugr / ml in PBS1X for 15 min at RT in the dark, washed again twice with PBS1X and mounted on a slide in Vectashield supplemented with Propidium iodide (Reactolab, Vector H- 1300). Samples were imaged with a Leica TCS SP8 Multiphoton microscope, equipped with RLD detectors, and in photon counting mode. In-situ hybridization The YFP digoxygenin-labeled antisense and sense RNA probes were generated by in vitro transcription according to the instructions provided with the DIG RNA labeling kit (SP6 / T7; Roche) using a plasmid containing the YFP sequence as template. Developing inflorescences or pollinated pistils and collected at the desired time points were fixed in FAA fixative (Formaldehyde, acetic acid, ethanol) o / n, then moved in ethanol 70% and into an ASP200 embedding machine for embedding (Leica Microsystems GmbH, Wetzlar, Germany), where they were dehydrated in a graded series of ethanol at room temperature (70% for 1 h, 3×90% for 1h, 3×99.98% for 1h, all at room temperature) followed by xylol (2×1 h and 1×1 h 15 min). Xylol was substituted by Paraplast X-tra embedding media (Roth AG, Arlesheim, Switzerland) at 58˚C (2×1 h and 1×3 h). The pistils were poured into small plastic tray while the paraffin was still liquid, and then let to set. For storage, the paraffin blocks containing the pistils have been stored at 4˚C. The samples were then sliced in 8-μm sections with a RM2145 Leica microtome (Leica Microsystems GmbH, Wetzlar, Germany) and then hybridized as described previously (Cañas, L. A. et al., Plant J.6, 597–604,1994), with strong formaldeyde washes, as described in Dreni et al. Plant J.52, 690–699, 2007. Images were taken with a Zeiss DMR microscope equipped with differential interference contrast (DIC) filters and Leica Flexacam C3 LSR camera. Clearing Siliques at different time points after pollination were fixed o / n in fixative (Ethanol:Acetic Acid 9:1 v / v) at room temperature. The following day, the fixative was replaced with Ethanol 70%. Seeds and ovules were isolated from the valves and mounted in Clearing solution (Chloral Hydrate:Water:Glycerol 4:2,5:1 w / v / w) and left to clear few hours or overnight depending on the size. Images were taken with a Zeiss DMR microscope equipped with differential interference contrast (DIC) filters and Leica Flexacam C3 LSR camera. Pollen tube speed assay Emasculated pistils were emasculated and pollinate the day after with pollen from the desired background. After 5 hours ovule were dissected and mounted on glucose 7% Propidium iodide 0.1mgr / ml. Ovule have been imaged at DM6000 Leica microscope by scoring the percentage of ovules with accumulation of Propidium Iodide in the synergid, a sign of occurred fertilization. Laser Capture MIcrodissection Transcriptome The protocol is based on the method published in Schmidt et al., 2012 ibid. Briefly, pistils obtained from the reciprocal crosses at precise time points were collected and fixed in ice-cold ethanol:acetic acid 9:1, and by applying vacuum for the initial 5 min by keeping the samples on ice. Afterwards, the samples were incubated in the fixative overnight at 4˚C. For long storage of the material, fixative have been replaced by with ethanol 100%, and samples were stored at -20˚C. For embedding, samples were transferred in ethanol 70%, and moved in an ASP200 embedding machine (Leica Microsystems GmbH, Wetzlar, Germany), where the tissue was dehydrated in a graded series of ethanol at room temperature (70% for 1 h, 3×90% for 1h, 3×99.98% for 1h, all at room temperature) followed by xylol (2×1 h and 1×1 h 15 min). Xylol was substituted by Paraplast X-tra embedding media (Roth AG, Arlesheim, Switzerland) at 58˚C (2×1 h and 1×3 h). The pistils were poured into small plastic tray while the paraffin was still liquid, and then let to set. For storage, the paraffin blocks containing the pistils have been stored at 4˚C. For microdissection, paraffin blocks containing the pistils were cut on a RM2145 Leica microtome (Leica Microsystems GmbH, Wetzlar, Germany) to 8um thin slices and mounted on nuclease-free membrane-mounted metal-frame slides using DEPC water. Slides were left to dry for 1 hour on a heating block at 38-40˚C. The slides were then submerged in Xylol to remove the paraffin, at room temperature, Samples were then deparaffinized in xylol at room temperature (2 washes of 20min each). Microdissection was performed using a mmi CellCut Plus device (MMI Molecular Machines & Industries AG, Glattbrugg, Switzerland). An average of 40 Central Cells were isolated per day using the special glued-cap eppendorf. After collection, 50ul of extraction buffer of the PicoPure RNA isolation kit (Arcturus Engineering, Mountain View, USA) was added to the eppendorf and incubated up-side down (so to have the buffer in contact with the cap of the tube) for 30min at 42˚C in a water bath to release the tissue from the membrane. At intervals, the sample was vortexed vigorously by keeping the eppendorf up-side down so to expose the sections to the buffer. The tube was then stored at –80’C for longer storage. Total RNA was isolated using the PicoPure RNA isolation kit (Arcturus Engineering, Mountain View, USA) following the manufacturer's instructions. Extracts from different tubes were pooled to reach a sufficient RNA yield. The inventors have combined together tubes to obtain an average of 300 Central Cells per each replicate. Total RNA was tested for integrity and quality using the Agilent TAPE Station, and converted to cDNA using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio), following the manufacturer’s instruction. Then libraries were constructed using the Nextera XT DNA Sample Preparation Kit (Illumina) following manufacturer’s instructions. Libraries were then sequenced at 150bp double paired-end, in a Nova-Seq Illumina machine, by combining all the 12 libraries in a single lane at the Functional Genomic Centre Zurich. Cited prior art documents: ^ Schmid, M. W. et al. A Powerful Method for Transcriptional Profiling of Specific Cell Types in Eukaryotes: Laser-Assisted Microdissection and RNA Sequencing. PLoS ONE 7, e29685 (2012) ^ Schmidt, A. et al. Transcriptome Analysis of the Arabidopsis Megaspore Mother Cell Uncovers the Importance of RNA Helicases for Plant Germline Development. PLoS Biol.9, e1001155 (2011) ^ Loraine, A. E., McCormick, S., Estrada, A., Patel, K. & Qin, P. RNA-seq of Arabidopsis pollen uncovers novel transcription and alternative splicing. Plant Physiol.162, 1092– 1109 (2013) ^ Weimer, A. K. et al. Lineage- and stage-specific expressed CYCD7;1 coordinates the single symmetric division that creates stomatal guard cells. Dev. Camb. Engl. 145, dev160671 (2018) ^ Cruz-Ramírez, A. et al. A bistable circuit involving SCARECROW-RETINOBLASTOMA integrates cues to inform asymmetric stem cell division. Cell 150, 1002–1015 (2012) ^ Biedermann, S. et al. The retinoblastoma homolog RBR1 mediates localization of the repair protein RAD51 to DNA lesions in Arabidopsis. EMBO J.36, 1279–1297 (2017). ^ Grützner, R. et al. High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns. Plant Commun.2, 100135 (2021). ^ Simonini, S. et al. The Polycomb group protein MEDEA controls cell proliferation and embryonic patterning in Arabidopsis. Dev. Cell 56, 1945-1960.e7 (2021). ^ She, W., Grimanelli, D. & Baroux, C. An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis. JoVE J. Vis. Exp. e51530 (2014) doi:10.3791 / 51530. ^ Cañas, L. A., Busscher, M., Angenent, G. C., Beltrán, J.-P. & Van Tunen, A. J. Nuclear localization of the petunia MADS box protein FBP1. Plant J.6, 597–604 (1994). ^ Dreni, L. et al. The D-lineage MADS-box gene OsMADS13 controls ovule identity in rice. Plant J.52, 690–699 (2007). Sequences The following sequences are RBR1 from Arabidopsis and orthologues, useful as targets for downregulation, as laid out in detail in the description. >A.thaliana (SEQ ID NO: 7): MEEVQPPVTPPIEPNGKRSEASLLDICEKVLSLDGSTCDEALKLFTETKRILSASMSNIGSGTREEVERFW FAFILYSVKRLSVRKEADGLSVSGDNEFNLCQILRALKLNIVDFFKELPQFVVKAGSVLGELYGADWENRL QAKEVQANFVHLSLLSKYYKRGFREFFLTYDANAEKNSANSSTYLLDSYRFGWLLFLALRNHAFSRFKDLV TCSNGVVSILAILIIHVPCRFRNFSIQDSSRFVKKGDKGVDLVASLCKIYDASEDELRIVIDKANNLVETI LKKKPSPASECQTDKLDNIDPDGLTYFEDLLEETSISTSLITLEKDYYDGKGELDERVFINEEDSLLGSGS LSAGAVNITGVKRKIDALSSPARTFISPLSPHKSPAAKTNGISGATKLAATPVSTAMTTAKWLRTVISPLL PKPSPGLEHFLKSCDRDITNDVTRRAHIILEAIFPNSSLGAQCGGGSLQAVDLMDDIWAEQRRLEACKLYY RVLEAMCKAEAQILHANNLNSLLTNERFHRCMLACSAELVLATHKTITMLFPAVLERTGITAFDLSKVIES FIRHEDSLPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPSLALEINQLGLLAEPMPSLDAIAALIN FSDGANHASSVQKHETCPGQNGGIRSPKRLCTDYRSILVERNSFTSPVKDRLLALGNVKSKMLPPPLQSAF ASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYCFFQHVLAQRTSLLFSRH IDQIILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVYVDALQCRRQGRIGPDHVDIITFYNEIF IPAVKPLLVELGPVRNDRAVEANNKPEGQCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKMDALIS HSTKSYYACVGESTHAYQSPSKDLSAINNRLNNSSSNRKRTLNFDAEAGMVSDSMVANSLNLQNQNQNQNG SDASSSGGAAPLKTEPTDS >B.napus_isoX2 (SEQ ID NO: 8): MEEVQPPVTPPIDPNGKRSEAVLLELCEKVLSLEGSICDEALKLFTETKPILSANMANIGSGTREEVERFW FAFVLYSVKMLTVRKQVDGQSVSGDNKFNLCQILRALKLNIVDFFKELPQFAVKAGPVLCELYGADWENRL QAKELQANFVHLSLLSKYYKRGYQEFFLTYDANAEKTSANSASYLPDSYRFGWLLFLALRNHAFSRFKDLV TCTNGLVSILAILIIHVPCRFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMDQANNLIETI LKKKPSSASTCKIDKLDNINPDGLTYFEDLLDETSISTSLITLEKDYEDSVCNKGELDERVFINEEDSLLG SGSLSAGAVNVTGVKRKIDSLSSPARTFISPLSPHKSPAAKTNTISGAIKLTATPVSTAMTTAKWLRTVIC PLLPKPSPGLEHFLKSCDRDITTDVTRRAHVILEAIFPNSSLGDRCAGGSLQPVNLMDDIWAEQRRLEAVK LYYRVLEAMCIAEAQILHANNLNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKV IESFIRHEDSLPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPLLALEINRLGLLAEPMPSLDAIAA LINFSEGSNHAPSIQKHETCPGQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLAFGNVKSKMLPPPLQ SAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYRLFQHVLAQRTSLLF NRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVYVDSHQSRRQGQGRVGPDHVDIITF YNEIFIPAVKPLLVEIIPVKKDQAMEANNKPEGHCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKM DALISHSSKSYYACVGESTHAYQSPSKDLSAINNRLNNCSTNRKRTLKFDAEAGLVSDSMVGNSLYLQNQN QNGSDASSSGGAPLKTEPVDSS >B.napus_isoX1 (SEQ ID NO: 9): MEEVQPPVTPPIDPNGKRSEAVLLELCEKVLSLEGSICDEALKLFTETKPILSANMANIGSGTREEVERFW FAFVLYSVKMLTVRKQVDGQSVSGDNKFNLCQILRALKLNIVDFFKELPQFAVKAGPVLCELYGADWENRL QAKELQANFVHLSLLSKYYKRGYQEFFLTYDANAEKTSANSASYLPDSYRFGWLLFLALRNHAFSRFKDLV TCTNGLVSILAILIIHVPCRFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMDQANNLIETI LKKKPSSASTCKIDKLDNINPDGLTYFEDLLDETSISTSLITLEKDYEDSVCNKGELDERVFINEEDSLLG SGSLSAGAVNVTGVKRKIDSLSSPARTFISPLSPHKSPAAKTNTISGAIKLTATPVSTAMTTAKWLRTVIC PLLPKPSPGLEHFLKSCDRDITTDVTRRAHVILEAIFPNSSLGDRCAGGSLQPVNLMDDIWAEQRRLEAVK LYYRVLEAMCIAEAQILHANNLNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKV IESFIRHEDSLPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPLLALEINRLGLLAEPMPSLDAIAA LINFSEGSNHAPSIQKHETCPGQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLAFGNVKSKMLPPPLQ SAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYRLFQHVLAQRTSLLF NRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVYVDSHQSRRQGQGRVGPDHVDIITF YNEIFIPAVKPLLVEIIPVKKDQAMEANNKPEGHCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKM DALISHSSKSYYACVGESTHAYQSPSKDLSAINNRLNNSCSTNRKRTLKFDAEAGLVSDSMVGNSLYLQNQ NQNGSDASSSGGAPLKTEPVDSS >B.napus_isoX3 (SEQ ID NO: 10): MEEVQPPVTPPIDPNGKRSEAVLLELCEKVLSLEGSICDEALKLFTETKPILSANMANIGSGTREEVERFW FAFVLYSVKMLTVRKQVDGQSVSGDNKFNLCQILRALKLNIVDFFKELPQFAVKAGPVLCELYGADWENRL QAKELQANFVHLSLLSKYYKRGYQEFFLTYDANAEKTSANSASYLPDSYRFGWLLFLALRNHAFSRFKDLV TCTNGLVSILAILIIHVPCRFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMDQANNLIETI LKKKPSSASTCKIDKLDNINPDGLTYFEDLLDETSISTSLITLEKDYEDSVCNKGELDERVFINEEDSLLG SGSLSAGAVNVTGVKRKIDSLSSPARTFISPLSPHKSPAAKTNTISGAIKLTATPVSTAMTTAKWLRTVIC PLLPKPSPGLEHFLKSCDRDITTDVTRRAHVILEAIFPNSSLGDRCAGGSLQPVNLMDDIWAEQRRLEAVK LYYRVLEAMCIAEAQILHANNLNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKV IESFIRHEDSLPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPLLALEINRLGLLAEPMPSLDAIAA LINFSEGSNHAPSIQKHETCPGQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLAFGNVKSKMLPPPLQ SAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYRLFQHVLAQRTSLLF NRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVYVDSHQSRRQGQGRVGPDHVDIITF YNEIFIPAVKPLLVEIIPVKKDQAMEANNKPEGHCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKM DALISHSSKSYYACVGESTHAYQSPSKDLSAINNRLNKYISFCGIAAAAPTARGRLNLTRKQDWSVIPW >B.napus_isoX4 (SEQ ID NO: 11): MEEVQPPVTPPIDPNGKRSEATLLDLCEKVLSLEGSVCDEVLKLFTETKRILSANMANIGSGTREEVERFW FAFILYSVKMLTVRKQVDGQSVSGDNGFNLCQILRALKLNIVDFFKELPQFVVKAGPVLCELYGADWENRL QAKELQANFVHLSLLSKYYKRGYREFFLTYDANAEKTSANSASYLPDSYRFGWLLFLALRNHAFSRFKDLV TCTNGLVSVLAILIIHVPCRFRNFSIQDSSRFVKKGDKDVDLVASLCKIYDASEDELRKIMNKANSLIETI LKKRPSPASTCKTDTLDNIITDGLTYFEDLLDESSISTSLTTLEKDYDETVCNKGELDERVFINEEDSLLG SGSLSAGAVNLTGAKRKIDSLSSPARTFISPLSPHKSPAAKTTVTATPVSTAMTTAKWLRTVICPLPPKPS PGLELFLKSCDRDITNDVTRRAHVILEAIFPNSSLGDQCAGGSLQPVNLMDDIWAQQRRLEAVKLYYRVLE AMCKAEAQILHVNNLNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLSKVIESFIRH EDSLPRELRRHLNSLEERLLESMVWERGSSMYNSLIVAKAPLALEINRLGLLAEPMPSLDAIAALINFSEG SSNHASSVQKHEACPGQNGDIKSPKRLCTDYRSILVERSSFASPVKERLLALGNVKSKTLPPPLQSAFATP TRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYCLFQRVLAQRTSLLFNRHIDQ IILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVFVDSSQGRRQGRVGPDHVDIITFYNDVFIPA VKPLLVEIVPVKKGQAVEANNNPEGHCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKMDALISHSS KSYYACVGESTHAYQSPSKDLSAINNRLNNSNNSSNRKRTLKFDVEAGLVSDSMVGNSLYQQNQNGSDASS SGGAPFKTEPQD >B.rapa_isoX1 (SEQ ID NO: 12): MEEVQPPVTPPIDPNGKRSEAVLLELCEKVLSLEGSICDEALKLFTETKPILSANMANIGSGTREEVERFW FAFVLYSVKMLTVRKQVDGQSVSGDNKFNLCQILRALKINIVDFFKELPQFAVKAGPVLCELYGADWENRL QAKELQANFVHLSLLSKYYKRGYQEFFLTYDANAEKTSANSASYLPDSYRFGWLLFLALRNHAFSRFKDLV TCTNGLVSILAILIIHVPCRFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMDQANNLIETI LKKKPSSASTCKIDKLDNINPDGLTYFEDLLDETSISTSLITLEKDYEDSVCNKGELDERVFINEEDSLLG SGSLSAGAVNVTGVKRKIDSLSSPARTFISPLSPHKSPAAKTNTISGAIKLTATPVSTAMTTAKWLRTVIC PLLPKPSPGLEHFLKSCDRDITTDVTRRAHVILEAIFPNSSLGDRCAGGSLQPVNLMDDIWAEQRRLEAVK LYYRVLEAMCIAEAQILHANNLNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKV IESFIRHEDSLPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPLLALEINRLGLLAEPMPSLDAIAA LINFSEGSNHAPSIQKHETCPGQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLAFGNVKSKMLPPPLQ SAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYRLFQHVLAQRTSLLF NRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVYVDSHQSRRQGQGRVGPDHVDIITF YNEIFIPAVKPLLVEIIPVKKDQAMEANNKPEGHCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKM DALISHSSKSYYACVGESTHAYQSPSKDLSAINNRLNNSCSTNRKRTLKFDAEAGLVSDSMVGNSLYLQNQ NQNGSDASSSGGAPLKTEPVDSS >B.rapa_isoX2 (SEQ ID NO: 13): MEEVQPPVTPPIDPNGKRSEATLLDLCEKVLSLEGSVCDEALKLFTETKRILSANMANIGSGTREEVERFW FAFILYSVKMLTVRKQVDGQSVSGDNGFNLCQILRALKLNIVDFFKELPQFVVKAGPVLCELYGADWENRL QAKELQANFVHLSLLSKYYKRGYREFFLTYDANAEKTSANSASYLPDSYRFGWLLFLALRNHAFSRFKDLV TCTNGLVSVLAILIVHVPCRFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMNKANSLIETI LKKKPSAASACKTDTLDNINTDGLTYFEDLLDESSISTSLTTLEKDYDETVCNKGELDERVFINEEDSLLG SGSLSAGAVNLTGAKRKIDSLSSPARTFISPLSPHKSPAAKTTVTATPVSTAMTTAKWLRTVICPLPPKPS PGLELFLKSCDRDITNDVTRRAHVILEAIFPNSSLGDQCAGGSLQPVNLMDDIWAQQRRLEAVKLYYRVLE AMCKAEAQILHVNNLNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLSKVIESFIRH EDSLPRELRRHLNSLEERLLESMVWERGSSMYNSLIVAKAPLALEINRLGLLAEPMPSLDAIAALINFSEG SSNHASSVQKAEACPGQNGDIKSPKRLCTDYRSILVERSSFASPVKERLLALGNVKSKTLPPPLQSAFATP TRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYCLFQRVLAQRTSLLFNRHIDQ IILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVFVDSSQGRRQGRVGPDHVDIITFYNDVFIPA VKPLLVEIVPVKKGQAVEANNNPEGHCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKMDALISHSS KSYYACVGESTHAYQSPSKDLSAINNRLNNNNSSNRKRTLKFDVEAGLVSDSMVGNSLYQQNQNQNQNETD ASNQNQNGSDASSSGGAPFKTEPQD >B.rapa_isoX3 (SEQ ID NO: 14): MEEVQPPVTPPIDPNGKRSEATLLDLCEKVLSLEGSVCDEALKLFTETKRILSANMANIGSGTREEVERFW FAFILYSVKMLTVRKQVDGQSVSGDNGFNLCQILRALKLNIVDFFKELPQFVVKAGPVLCELYGADWENRL QAKELQANFVHLSLLSKYYKRGYREFFLTYDANAEKTSANSASYLPDSYRFGWLLFLALRNHAFSRFKDLV TCTNGLVSVLAILIVHVPCRFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMNKANSLIETI LKKKPSAASACKTDTLDNINTDGLTYFEDLLDESSISTSLTTLEKDYDETVCNKGELDERVFINEEDSLLG SGSLSAGAVNLTGAKRKIDSLSSPARTFISPLSPHKSPAAKTTVTATPVSTAMTTAKWLRTVICPLPPKPS PGLELFLKSCDRDITNDVTRRAHVILEAIFPNSSLGDQCAGGSLQPVNLMDDIWAQQRRLEAVKLYYRVLE AMCKAEAQILHVNNLNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLSKVIESFIRH EDSLPRELRRHLNSLEERLLESMVWERGSSMYNSLIVAKAPLALEINRLGLLAEPMPSLDAIAALINFSEG SSNHASSVQKAEACPGQNGDIKSPKRLCTDYRSILVERSSFASPVKERLLALGNVKSKTLPPPLQSAFATP TRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYCLFQRVLAQRTSLLFNRHIDQ IILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVFVDSSQGRRQGRVGPDHVDIITFYNDVFIPA VKPLLVEIVPVKKGQAVEANNNPEGHCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKMDALISHSS KSYYACVGESTHAYQSPSKDLSAINNRLNNSNRKRTLKFDVEAGLVSDSMVGNSLYQQNQNQNQNETDASN QNQNGSDASSSGGAPFKTEPQD >B.rapa_isoX4 (SEQ ID NO: 15): MEEVQPPVTPPIDPNGKRSEATLLDLCEKVLSLEGSVCDEALKLFTETKRILSANMANIGSGTREEVERFW FAFILYSVKMLTVRKQVDGQSVSGDNGFNLCQILRALKLNIVDFFKELPQFVVKAGPVLCELYGADWENRL QAKELQANFVHLSLLSKYYKRGYREFFLTYDANAEKTSANSASYLPDSYRFGWLLFLALRNHAFSRFKDLV TCTNGLVSVLAILIVHVPCRFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMNKANSLIETI LKKKPSAASACKTDTLDNINTDGLTYFEDLLDESSISTSLTTLEKDYDETVCNKGELDERVFINEEDSLLG SGSLSAGAVNLTGAKRKIDSLSSPARTFISPLSPHKSPAAKTTVTATPVSTAMTTAKWLRTVICPLPPKPS PGLELFLKSCDRDITNDVTRRAHVILEAIFPNSSLGDQCAGGSLQPVNLMDDIWAQQRRLEAVKLYYRVLE AMCKAEAQILHVNNLNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLSKVIESFIRH EDSLPRELRRHLNSLEERLLESMVWERGSSMYNSLIVAKAPLALEINRLGLLAEPMPSLDAIAALINFSEG SSNHASSVQKAEACPGQNGDIKSPKRLCTDYRSILVERSSFASPVKERLLALGNVKSKTLPPPLQSAFATP TRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYCLFQRVLAQRTSLLFNRHIDQ IILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVFVDSSQGRRQGRVGPDHVDIITFYNDVFIPA VKPLLVEIVPVKKGQAVEANNNPEGHCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKMDALISHSS KSYYACVGESTHAYQSPSKDLSAINNRLNNNRKRTLKFDVEAGLVSDSMVGNSLYQQNQNQNQNETDASNQ NQNGSDASSSGGAPFKTEPQD >B.oleracea_isoX2 (SEQ ID NO: 16): MEEVQPPVTPPIDPNGKRSEAILSDLCEKVLSLEGSSCDEALKLFTETKPILSANMANIGSGTREEVERFW FAFVLYSVKMLTVRKQVDGQSVPVDNRFNLCQILRALKLNIVDFFKELPQFVVKAGPVLCELYGADWENRL QAKELQANFVHLSLLSNYYKRGYQEFFLTYDANAERASANSASYLPDSYRFGWLLFLALRNHAFSRFKDLV TCTNGLVSILAILIIHVPCRFRNFNIQDSSRFVKKGDKEVDLVASLCKIYDASEDELKKIMDQANNLIETI LKKKPSSASTCKIDKLDNINPDGLTYFEDLLDEASISTSSITLEKDYEDSVCNKGELDERVFINEEDSLLG SGSLSAGAVNVTGVKRKIDSLSSPARTFISPLSPHKSPAAKTNTISGAIKLTATPVSTAMTTAKWLRTVIC PLLPKPSPGLEHFLKSCDRDITTDVTRRAHVILEAIFPNSSLGDRCAGGSLQPVNLMDDIWAEQRRLEAVK LYYRVLEAMCIAEAQILHANNLNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKV IESFIRHEDSLPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPSLALEINRLGLLAEPMPSLDAIAA LINFSEGSHHAPSVQKHETCPGQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLAFGNVKSKMLPPPLQ SAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYRLFQHVLAQRTSLLF NRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVYVDSHQSRRQGQWRVGPDHVDIITF YNEIFIPAVKPLLVEIIPVKKDQAMEANNKAEGHCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKM DALVSHNSKSYYACVGESTHAYQSPSKDLSAINNRLNNCSTNRKRTLKFDVEAGLVSDSMVGNSLYLQNQN GSDASSSGGAPLKTEPVDSS >B.oleracea_isoX1 (SEQ ID NO: 17): MEEVQPPVTPPIDPNGKRSEAILSDLCEKVLSLEGSSCDEALKLFTETKPILSANMANIGSGTREEVERFW FAFVLYSVKMLTVRKQVDGQSVPVDNRFNLCQILRALKLNIVDFFKELPQFVVKAGPVLCELYGADWENRL QAKELQANFVHLSLLSNYYKRGYQEFFLTYDANAERASANSASYLPDSYRFGWLLFLALRNHAFSRFKDLV TCTNGLVSILAILIIHVPCRFRNFNIQDSSRFVKKGDKEVDLVASLCKIYDASEDELKKIMDQANNLIETI LKKKPSSASTCKIDKLDNINPDGLTYFEDLLDEASISTSSITLEKDYEDSVCNKGELDERVFINEEDSLLG SGSLSAGAVNVTGVKRKIDSLSSPARTFISPLSPHKSPAAKTNTISGAIKLTATPVSTAMTTAKWLRTVIC PLLPKPSPGLEHFLKSCDRDITTDVTRRAHVILEAIFPNSSLGDRCAGGSLQPVNLMDDIWAEQRRLEAVK LYYRVLEAMCIAEAQILHANNLNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKV IESFIRHEDSLPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPSLALEINRLGLLAEPMPSLDAIAA LINFSEGSHHAPSVQKHETCPGQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLAFGNVKSKMLPPPLQ SAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYRLFQHVLAQRTSLLF NRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVYVDSHQSRRQGQWRVGPDHVDIITF YNEIFIPAVKPLLVEIIPVKKDQAMEANNKAEGHCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKM DALVSHNSKSYYACVGESTHAYQSPSKDLSAINNRLNNSCSTNRKRTLKFDVEAGLVSDSMVGNSLYLQNQ NGSDASSSGGAPLKTEPVDSS >N.bentamiana (SEQ ID NO: 18): MVELNNCSNSEETGGVDSLEVRFTDFCKNGLSMGESFVTEATKLFNDSKHLLLSNNSTIGVITQEGVERYW FVFVLYSVKRLSEKEAGNSNNGDKGNAFSLCQILRGAKLNVVDFFKELPQFILKVGPTLSNLYGSDWEKRL EVKELQTNFVHLSLLSKYYKRAYQELFLASGNNEDKPSATSNSAIHLPQYYRFGWLLFLSLRIHVFSRFKD LVTCTNGLVSVLAILMIHVPVRFRNFNIDDSSRFVKKGDKVDLLASLSKIYQTSIDDLRETMDKVNNLITE KLKKKPCLASEFRTENLDNLDTDGLTYFEDLMEESCLSSSVSTLEKDYSDAIQNKGELDERIFVNEEDSLL GSGSLSGGAVNMNGTKRKFDAMASPAKTVTSTLSPYRSPNCANSKMTAATPVSTAMTTARWLRTVIAPLQP KPSAELERFLSSCDRDVTADVIRRAQIILEAIFPSSGPAEHCAAGGSLQSTSLMDNIWAEQRRSEALKLYY RVLQTMCTAESQILNGNNLTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAFDLSKVIES FIRHEESLPRELRRHLNSLEERLLESMVWEKGSSMYNSLAVAKPSLAAEINRMGLLAEPMPSLDAIAMHIN LSSGSLPPLPSLHKNNLAPNGQIGDIRSPKKVCSEYRSVLVERNSFTSPVKDRFLALNNIKSKFPPPALHS AFASPTRPNPGGGGETCAETAINVFFGKIVKLAAVRINGMIERLQLSQQIRETVYCLFQKILSQRTSLFFN RHIDQIILCSFYGVAKISQLNLTFKEIICNYRKQPQCKPQVFRSVFVDWTLARHNVRTGADHVDIITFYNE MFIPSVKPLLVELAPAGNNSEKNDHNDGQGPASPKPSPFPKLPDMSPKKVSAVHNVYVSPLRASKMDALIS HSSKSYYACVGESTHAYQSPSKDLDVINNRLNGNRKLRGALNFDVDAGLVSDSIVANSLYLQNGNCRSPVA HVKTEQPES >N.tabacum (SEQ ID NO: 19): MVELNNCSNSEENGCVDSLEVRFTDFCKNGLSMGESFVTEATKLFNDSKHLLLSNNSTIGVITQEGVERYW FVFVLYSVKRLSEKEVGNSSNGNEGNAFSLCQILRGAKLNVVDFFKELPQFILKVGPTLSNLYGSDWEKRL EAKELQTNFVHLSLLSKYYKRAYQELFLASGNNEDKPSATSSSAIHLPQYYRFGWLLFLSLRIHVFSRFKD LVTCTNGLVSVLAILIIHVPVRFRNFNINDSSRFVKKGDKVDLLASLGKIYQTSIDDLRETMDKVNYLITE KLKKKPCLASEFRTENLDNLDTDGLTYFEDLMEESCLPSSVSILEKDYSDAIQNKGELDERIFVNEEDSLL GSGSLSGGVVNMNGVKRKFDAMASPAKTVTSTLSPYRSPNCANSKMTVATPVSTAMTTARWLRTVIAPLQP KPSAELERFLSSCDRDVTADVIRRAQIILEAIFPSSGPAEHCAAGGSLQSTSLMDNIWAEQRRSEALKLYY RVLQTMCTAESQILNGNNLTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAFDLSKVIES FIRHEESLPRELRRHLNSLEERLLESMVWEKGSSMYNSLAVAKPSLAAEINRTGLLAEPMPSLDAIAMHIN LSSGSLPPLPSLHKNNLAPNGQIGDIRSPKKVCSEYRSVLVERNSFTSPVKDRFLALNNIKSKFPPPALQS AFASPTRPNPGGGGETCAETAINVFFGKIVKLAAVRINGMIERLQLSQQIRETVYCLFQKILSQRTSLFFN RHIDQIILCSFYGVAKISQLNLTFKEIICNYRKQPQCKPQVFRSVFVDWTLARHNVRTGADHVDIITFYNE MFIPSVKPLLVELAPAGNAEKNNHNDGQGPASPKPSPFPKLPDMSPKKVSAVHNVYVSPLRASKMDALISH SSKSYYACVGESTHAYQSPSKDLDVINNRLNGNRKLRGALNFDVDAGLVSDSIVANSLYLQNGNCRSPVAH VKTEQPES >S.tuberosum (SEQ ID NO: 20): MNMEELKNYSNSEENGSVDSLEVRFTDFCKNGLSMGESFMVEARKLFMDSKHLLLSNNAAIGAITPEEVER YWFAFVLFSVKRLSENEARNPSNGNEGKGFDLCQILRGAKLNVLDFFKELPQFIVKVGPVLSNLYGSDWEK RLQAKELQTNFVHLSLLSKYYKRAYQELFLSSDNNEDKQSATSNSIILLPQYYRFGWLLFLSLREHVFSRF KDLVTCTNGLVSVLAMLIIHVPVRYRNFNINDSPRFVKKGDKVDLLVSLSSIYQTSIDDLRETMDKVNNLI TVKLKKEPCLASECRAENLDNIDADGLTYFENLLEESSLSSSICILEKDYNDAIQNKGELDERIFVNDEDS LLGSGSLSGGAVNMNGIKKKFDAMASPTKTITSPLSPYRSPGASNVNNNPNCGNSKMAATPVTTAMTTARW LRTVIAPLQPKPSPELERFLSACDRNVSADVIRRAHIILEAIFPSSGPGEHCAAGSLQSTSLMDNIWAEQR RSESLKLYYRVLQTMCVAESQILHATNLTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITS FDLSKVIESFIRHEESLPRELRRHLNSLEERLLESMVWEKGSSMYNSLTVAKPSLSAEINRLGLLAEPMPS LDAIAMHINLSSGNLPPVPPLQKSDLAPNGHNCDIRSPKRLCTEYRSVLVERNSFTSPVKDRFLALTNIKS KFPPPLQSAFASPTRPNPGGGGETCAETSINVFFGKIVKLAAVRINGMIERLQLSQQIRETVYCLFQKILN QRTSLFFSRHIDQIILCSFYGVAKISQLNLTFKEIIYNYRKQPQCKPQVFRSVFVDWTSARHNGKTGSEHV DIITFYNEMFIPSVKPLLVELAPAGNEQKNNHVEKTKNDGQGPASPRPSSFPSLPDMSPKKVSAVHNVYVS PLRSSKMDALISHSSKSYYACVGESTHAYQSPSKDLTVINNRLNGNRKLRGALNFDDVDAVGLVSDSIVAN TLYLQNGNCISSPRAAVKTEQPES >S.pennellii (SEQ ID NO: 21): MNMEELKNHSNSEENGSVDSLEVRFTDFCKNGLSMGESFMVEARKLFMDSKHLLLSNNAAIGAITPEEVER YWFVFVLFSVKRLSENEARNSSNGNEGNGFDLCQILRGAKLNVLDFFKELPQFIVKVGPVLSNLYGSDWEK RLQAKELQTNFVHLSLLSKYYKRAYQELFLSSDNNEVNQSATSNSIVLLPQYYRFGWLLFLSLREHAFSRF KDLVTCTNGLVSVLAILIIHVPVCFRNFNINDSPRFVKKGDKVDLLVSLSSIYQTSIDDLRETMDKVNNLI TVKLKKELCLASECRAKNLDNIDADGLTYFENLLEESSLSSSICILEKDYNDAIQNKGELDERIFVNDEDS LLGSGSLSGGAVNMNGIKKKFDAMASPTKTITSPLSPYRSPGASNVNSNPNCGNSKMAATPVTTAMTTARW LRTVIAPLQAKPSPELERFLSACDRNVSADVIRRAHIILEAIFPSSGPGEHCAAGSLQSTSLMDNIWAEQR RSESLKLYYRVLQTMCVAESQILHATNLTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITS FDLSKVIESFIRHEESLPRELRRHLNSLEERLLESMVWEKGSSMYNSLTVAKPSLSAEINRLGLLAEPMPS LDAIAMHINPSLGNLPPVPPLQKSDLAPNGHICDIRSPKRLCTEYRSVLVERNSFTSPVKDRFLALTNIKS KFPPPLQSAFASPTRPNPGGGGETCAETSINVFFGKIVKLAAVRINGMIERLQLSQQIRETVYCLFQKILS QRTSLFFSRHIDQIILCSFYGVAKISQLNLTFKEIIYNYRKQPQCKPQVFRSVFVDWTSARHNGKTGSEHV DIITFYNEMFIPSVKPLLVELAPAGNEQKNNHVEKTKNDGQGPASPRPSSFPSLPDMSPKKVSAVHNVYVS PLRSSKMDALISHSSKSYYACVGESTHAYQSPSKDLTVINNRLNGNRKLRGALNFDDVDAVGLVSDSIVAN TLYLQNGNCISSPRAAVKTEQPEP >S.lycopersicum (SEQ ID NO: 22): MNMEELKNHSNSEENGSVDSLEVRFTDFCKNGLSMGESFMVEARKLFMDSKHLLLSNNAAIGAITPEEVER YWFVFVLFSVKRLSENEARNSSNGNEGNGFDLCQILRGAKLNVLDFFKELPQFIVKVGPVLSNLYGSDWEK RLQAKELQTNFVHLSLLSKYYKRAYQELFLSSDNNEVNQSATSNSIVLLPQYYRFGWLLFLSLREHVFSRF KDLVTCTNGLVSVLAILIIHVPVCFRNFNINDSPRFVKKGDKVDLLVSLSSIYQTSIDDLRETMDKVNNLI TVKLKKELCLASECRAKNLDNIDADGLTYFENLLEESSLSSSICILEKDYNDAIQNKGELDERIFVNDEDS LLGSGSLSGGAVNMNGIKKKFDAMASPTKTITSPLSPYRSPGASNVNSNLNCGNSKMAATPVTTAMTTARW LRTVIAPLQAKPSPELERFLSACDRNVSADVIRRAHIILEAIFPSSGPGEHCAAGSLQSTSLMDNIWAEQR RSESLKLYYRVLQTMCVAESQILHVTNLTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITS FDLSKVIESFIRHEESLPRELRRHLNSLEERLLESMVWEKGSSMYNSLTVAKPSLSAEINRLGLLAEPMPS LDAIAMHINPSLGNLPPVPPLQKSDLAPNGHICDIRSPKRLCTEYRSVLVERNSFTSPVKDRFLALTNIKS KFPPPLQSAFASPTRPNPGGGGETCAETSINVFFGKIVKLAAVRINGMIERLQLSQQIRETVYCLFQKILS QRTSLFFSRHIDQIILCSFYGVAKISQLNLTFKEIIYNYRKQPQCKPQVFRSVFVDWTSARHNGKTGSEHV DIITFYNEMFIPSVKPLLVELAPAGNEQKNNHVEKTKKDGQGPASPRSSSFPSLPDMSPKKVSAVHNVYVS PLRSSKMDALISHSSKSYYACVGESTHAYQSPSKDLTVINNRLNGNRKLRGALNFDDVDAVGLVSDSIVAN TLYLQNGNCISSPRAAVKTEQPEP >G.max_isoX1 (SEQ ID NO: 23): MSPPPAESNAMEDGKSENGGDQVESRFFEFCKNGLALEEKSCKEAANLFGETKHILLSNFSSMGNGTSEEA ERYWFAFILYSVKKLIQKNDEGEKEDTENIGLSLCRILRAAKLNIADFFKELPQFVVKAGPTLSNLYGTDW ENRLEAKEMHANAIHLKILSKYYKRVFGEFFVATDTNAEINSPITVHASEYHRFGWLLFLALRVHAFSRFK DLVTCTNGLISILAILIIHVPTRFWNFNIHDSSRFVKKSNKGVDLLASLCNIYNTSEDELRKTMEKANNVI ADILKKQPCLASECETGNLENIDKDGLTYFKDLMEESSLPSSLSMLEKDYDYMIHNKSELDERLFINEDDS LLASVSLSGGSVSAGGVKRKFDSMASPAKTITSPLSPHRSPASHANGIPGSANSKMAATPVSTAMTTAKWL RTVISPLPSKPSPELERFLTSCDRDATSDVVRRAQIILQAIFPSSPLGERCVTGSLQSANVVDNIWAEQRR LEALKLYYRVLEAMCRAEAQVLHATNLTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAF DLSKVIESFIRHEESLPRELRRHLNSLEERLLESMVWEKGSSMHNSLAVARPSLSAEINSLGLLAEPMPSL DEIAMHINFSCGGLPPVPTLPKLESPSNQNGDIRSPKRNVLMERNSFTSPVKDCLLPCIILKSKLPPPPLQ SAFASPTKPNPGGGGETCAETGINIFFGKIIKLGAVRISGMVERLQLSQQIRENVYSLFQRILNQWTSLFF NRHIDQIILCCFYGVAKISQLNLTFKEIVYNYRKQPHCKPQVFRSVFVDWSLARRNGRTGQEHIDIITFYN EIFIPSVKPLLVELGPAGPTTKSDRIPEVNKNDGHLAQCPGSPKISPFPTLPDMSPKKVSATHNVYVSPLR SSKMEALISHSSKSYYACVGESTHAYQSPSKDLTAINNRLNGNRKVRGPLNFDDVDFGLVSDSMVANSLYL QNGSCASSSGAPFKSEQPDS >G.max_isoX2 (SEQ ID NO: 24): MSPPPAESNAMEDGKPENGGDQVESRFFEFCKNGLALEEKSCKEATNLFGETKHILLSNFSSMGNGTSEEA ERYWFAFILYSVKKLIQKNDEGEKEDTENTGLSLCRILRATKLNIADFFKELPQFVVKAGPTLSNLYGTDW ENRLEAKEMHANAIHLKILSKYYKRVFGEFFVATDTNAEINSPITVHASEYHRFGWLLFLALRVHAFSRFK DLVTCTNGLISILAILIIHVPTRFRNFNIHDSSRFVKKSNKGVDLLASLCNIYNTSEDELRKTMEKANNVI ADILKKQPCLASECETENLENIDKDGLTYFKDLMEESSLPSSLSMLEKDYDYMIRNKSELDERLFINEDDS LLASVSLSGGSVSAGGVKRKFDSMASPAKTITSPLSPHRSPASHANGIPGSANSKMAATPVSTAMTTAKWL RTVISPLPSKPSPELERFLTSCDRDATSDVVRRAQIILQAIFPSSPLGERCVTGSLQSANLMDNIWAEQRR LEALKLYYRVLEAMCRAEAQVLHATNLTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAF DLSKVIESFIRHEESLPRELRRHLNSLEERLLESMVWEKGSSMYNSLAVARPSLSAEINRLGLLAEPMPSL DEIAMHINFSCGGLPPVPTLPKLESSSNQNGDIRSPKRNVLMERNSFTSPVKDRLLPFNSLKSKLPPPPLQ SAFASPTKPNPGGGGETCAETGINIFFGKIIKLGAVRISGMVERLQLSQQIRENVYSLFQRILNQWTSLFF NRHIDQIILCCFYGVAKISQLNLTFKEIVYNYRKQPHCKPQVFRSVFVDWSLARRNGRTGQEHIDIITFYN EIFIPSVKPLLVELGPAGPTTKSDRIPEINKNDGHLAQCPGSPKISPFPTLPDMSPKKVSATHNVYVSPLR SSKMEALISHSSKSYYACVGESTHAYQSPSKDLTAINNRLNGNRKVRGPLNFDDVDIGLVSDSMVANSLYL QNGSCASSSGAPLKSEQPDS >G.max_isoX3 (SEQ ID NO: 25): MEDGKPMNGGDQVESRFVEFCKNGLALEEKSCKKAKNLFGETKHILLSNFSSMGNGTSEEAERYWFAFILY SVKKLIQKNEEGGKEDAENTGLSLCRILRAAKLNIADFFKELPQFVVKAGPTLSNLYGTDWENRLEAKEMH ANAIHLKILSKYYKRVFGEFFVSTDTNAEKNSSITVHASEYHRFGWLLFLALRVHAFSRFKDLVTCTNGLI SILAILIIHVPTRFRNFNIHDSSRFVKKSNKGVDLLASLCNIYNTSEDELRKTMEKANNVIADILKKQPCL ASECETENLENIDKDGLTYFKDLMEESSLPSSLNMLEKDYDYMIRNKSELDERLFINEDDSLLASVSLSGG SVSVGGVKRKFDSMASPAKTITSPLSPHRSPTSHTNGIPGSANSKMAATPVSTAMTTAKWLRTVISPLPSK PSPELERFLTSCDRDVTSDVVRRAQIILQAIFPSSPLGERCVTGSLQSANLMDNIWAEQRRLEALKLYYRV LEAMCRAEAQVFHATNLTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAFDLSKVIESFI RHEESLPRELRRHLNSLEERLLESMVWEKGSSMYNSLAVARPSLSAEINRLGLLAEPMPSLDEIAMHINFS CGGLPPVPTLPKLESSPTQNGDIRSPKRNVLMERNSFTSPVKDRLLPFNSLKSKLPPPPLQSAFASPTKPN PGGGGETCAETGINIFFGKIIKLGAVRISGMVERLQLSQQIRENVYCLFQRILNQWTSLFFNHHIDQIILC CFYGVAKISQLNLTFKEIVYNYRKQPHCKTQVFRSVFVDWSLARRNGRTGQDHVDIITFYNEIFIPSVKPL LVELGPAGPTPKSDRIPEVNNKNEGHLAQCPGSPKISPFPTLPDMSPKKVSATHNVYVSPLRSSKMEALIS HSSKSYYACVGESTHAYQSPSKDLTAINNRLNGNRKVRGPLNFDDVDVGLVSDSMVANSLYLQNGSCASSS GAPLKSEQPDS > O.vulgare_isoX1 (SEQ ID NO: 26): MEPQPPPPPAVETHFADLCKELGVDEGVASEAAALLEEGKGVFLTPPLFGSKSPEDVERLCFAFVLYCVAK LKGKGMKEESSRVRLWKILEGCKLNYNDFFKGSQQLLSKLDHVLRSRYGTDWEDLLELKQLQSLVNLLADA SRFYCKAFNELFLSASTGQEPGSTTSNPDFFCFGWLFFLALRSKSPELFKDLVSCIHGLVAILAILLIHVP AKFRSFTIEGSSHLIKQTVRGVDLLASLCHNYHTSENRLKEMMGKFHKAIEEFFSTKAVRASECKTETSGK IDTDGLIYFRDLLDKECFHSNFEKLEKLTTTTSWEGELDLEMFLVNNGNIISAENSSRDFSNLSCPKRVFE TLASPAKTIKNMLTVPSSPSSPANGGSVKVVQMTPVTSAMTTAKWLHEVISSLPEKPSTKLEKFLSSCDTD LTSDVTKRVSIILEAIFPTKPSGHCGGSMGLNCTNAFDIPWAEARKMEASKLYYRVLEAVCRAESLNTNVN NLTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEESLPRELKRHLN SLEEQLLESMAWEKGSSLYNSLIVARPSLASEINRLGLLAESMLSLDDIVARQNFHAEDLPATPSKKQAAD PDENGDTRSPKRLCIESRSTLVDQIPQTPSAKQSYTLKAKWCPLQSTFASPTVSNPVGRNEKCAEVGVHIF FSKILKLAAIRIRNLCERLGHVEQTERVYNIFKQILDQQTALFFNRHIDQLILCSLYGVAKVSQLTLTFKE IVNNYKREQQCMPEVFRSVFVVNTDHNGGLGSRHVDIIIFYNEVFVPAVKPFLVALIPSGAHPDNKKNHNS QIPGSPKSSPFSNLPDMSPKKVSSSHNVYISPLRQTKKDALLSPSSRSFYACIGESTHAYQSSSEDLAAIN NCLNYTTRRINTRINFDMVSDSVVAGSLGQPNGVPASSDPAGSFSFLSRKGNPGPDS > O.vulgare_isoX2 (SEQ ID NO: 27): FPPAPPPRPFSSPPRRPTATTLLPARRSPDSVRPRSSYWGRQPMDGADAPQPARSGSGVTASTAPGRMNGG ASANANAAAMEERFADVCKRKLVLDESTTRQAMQLFKETKIILLSSMSSLGSGSPEEIERSWSACVLYCVS KLGNAGKAKEDRAITLRQILRAFELKIVDFFKEMPQFCIKVGFILTGLYGSDWEKRLELQELQANLVHLCS LGRQHYRRAYQELFLLNDGKPANNSSDLNLQQASEYYNFGWLLFLVLRNQASSAVKNLLTSTTELVSVLAV LIIHIPVRLRNFSIEDSSCFAKKSEKGVNLIASLCERYLTSEDELSKALQKTNILITDILKKKPCSDVSEC QQGCLSFIDPEGLVFFKNFLEEDSLKSSLQVLEREYENGLDTKGELDARMFANDEDSLLGSGSLSGGALKL PGTKRKYDDVMASPTKSTASRAPMSPPRFCPSPNGNSFCNSKMAPFTPVSTAMTTAKWLRSTISPLPSKPS GELLRFFSACDKDVTDDITCRAAIILGAIFTGSSFGERMCTSLRNTNGMDAIWTEQRKMEALKLYYRVLES MCRAESQILSGSNLTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHE DTLPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPTLSLEINRLGLLAEPMPSLDAIAVHHDISLGG LPPLPFQKQERSPGNKDEVRSPKRACTERRNVLADNSFRSPVKDAIKSKFLPPLQSAFASPTRPNPAAGGE TCAETGIGVFLSKITKLAAIRIKCLCERLQLSQQILERVYSLAQQIISQQTALFFNRHIDQIILCCIYGVA KISQLALTFKEIIFSYRKQSQCKPQVFRSVYVNWPSRSRSGKIGEDHVDIITFYNEVFIPTVKPLLVDLGP GTSPNRNNEPKSGGDAPFPESPRLSRFPNLPDMSPKKVSATHNVYVSPLRSSKMDTLLSPSSKSYYACVGE STHAFQSPSKDLNAINTRLNSGKKVNGRLNFDVVSDLVVARSLSDQNDGSASAMAVFGTKTPVKGEQPDP >O.sativa_isoX1 (SEQ ID NO: 28): MEGAAPPASSGSEVTGAGSGKVDAGGGAAMEERFADLCKSKLGLDESITRQAMQLFKESKSILLSSMSSLG SGSPEEIERFWSAFVLYCVSRLGKAGKGKEDGGISLCQILRAFSLNIVDFFKEMPQFCIKVGSVLAGLYGS DWEKRLELKELQANVVHLSLLSRYYKRAYQELFLLNDAKPPENSAEPNAQASDYYRFGWLLFLVLRIQTFS RFKDLVTSTNGLVSVLAVLIVHIPVRLRNFNIKESSSFAKKSDKGVNLIASLCEKYHTSEDELSKAIEKTN TLIVDILKKKPCPAASECQQDRLSFIDPEGLTYFKNLLEEDSLKLSLLMLEKEYENAINTKGELDERMFAN DEDSLLGSGSLSGGAINLPGTKRKYDVMASPAKSITSPSPMSPPRFCASPTGNGYCSSKMAPITPVSTAMT TAKWLRSTISPLPSKPSGELLRFFSACDKDVTDDITRRAGIILGAIFTSSSFGERICTSVRSTNRIDAIWT EQRKMEALKLYYRVLESMCRAETQILSGNNLTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTG ITAFDLSKVIESFVRHEDTLPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPTLSAEINRLGLLAEP MPSLDAIAAHHNISLEGLPPLPFQKQEHSPDKDEVRSPKRACTERRNVLVDNNSFRSPVKDTLKSKLPPLQ SAFLSPTRPNPAAGGELCAETGIGVFLSKIAKLAAIRIRGLCERLQLSQQVLERVYSLVQQIIIQQTALFF NRHIDQIILCSIYGVAKISQLALTFKEIIFGYRKQSQCKPQVFRSVYVHWASRSRNGKTGEDHVDIITFYN EVFIPTVKPLLVELGSGTSPNKKNEEKCAADGPYPESPRLSRFPNLPDMSPKKVSAAHNVYVSPLRTSKMD TLLSPSSKSYYACVGESTHAFQSPSKDLKVINNRLNSGKKVSGRLNFDVVSDLVVARSLSDQNSASAAATT ADIATKTPVKLEQPDC > O.sativa_isoX2 (SEQ ID NO: 29): MASQPPAAVEARLADLCKELGVDEGVAGEAAAVLEEGKGALLASPSFGSKSPEDAEKLCFAFVLYCVSKLK ETKAGSSGVRLWEILKGCKLKYDDFFKESQRLASRIDQVLGSRYGSDWEARLELKQLENLVNLLADASRFY CKAFNELFLSPSTDQEPGSTTNIPDYIRFGWLLFLILRSKSPELFKDLVSCIHGLVAILAILLIHVPAKFR SFTIEGSSHLIKQTEKGVDLLPSLCHNYHTSEDRLKEMMGKSYKVIEVFFSRKAINASEFKTVNLDKIDTD GLMYFKDLVDDEIFQSNLEKLEKLSSTTGCQGELDLEMFLTSNDYVLNAENSSGSSANFGCSKRVFETLAS PTKTIKNMLAAPSSPSSPANGGSIKIVQMTPVTSAMTTAKWLRDVISSLPDKPSSKLEEFLSSCDTDLTSD VVKRVSIILEAIFPTKSIDRGTSIGLNCANAFDIPWAEARKMEASKLYYRVLEAICRAESQNNNVNNLTPL LSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEETLPRELKRHLNSLEEQ LLESMSWEKGSSLYNSLVVARPSLSTEINSLGLLAEPMPSLDGIVARQSIHPDGLPPTPSKRWPSAGPDGN CYPQSPKRLCTESRNSLVERNSQTPPPKQSQTGLSILKAKYHPLQATFASPTVSNPVSGNEKCAVVGVQIF FSKILKLAAIRIRNLCERLRHEELTVSVYNIFKQILDQQTALFFNRHVDQIILCCLYGVAKVSQLSLTFKE IVNNYKREPQCKPEVFRSIFVGSTNRNGGFGSRHVDIIVFYNQVFVPTVKPLLVALMPSSTRPEDKRNTNS QIPGSPKSSPFSNLPDMSPKKVSSSHNVYVSPLRQTKMDALLSPSSRSFYACIGESTQAFQSPSKDLAAIN SRLNYPTRRINTRINFDMVSDSVVAGSLGQPNGGSASSDPAAAFSPLSKKSKTDS >S.bicolor_isoX1 (SEQ ID NO: 30): MSSPDPSPSTSTQQPKQLEGVTVLLKEASRFYRRAYNELFSGGTTEQEPESSTSTPEYMLFGWHLFLMLRS RSPELFKDLVSCIHGLVAVLAILLIHVPAKFRRFMIDGSSHLIKQNEKGMDLIASLCHNYHASEECVKEMM DKSHKAIEDIFGTKALSASEGKTENLDKIDTDGLMYFKGLIDTECFQSNLEKMEKLCNPNSCEGELDYKSI LINNDYIPCAENSPGDSTNLGHSKRVFEILASPTKTIKNMLTVPSSPLSPATGGSVKILQMTPVTSAMTTA KWLREVISSLPEKPSSKLQQLLSSCDRDLTNAVTERVSIVLEAIFPTKSSADRGGSLGISCANAFDIPWAE ARKMEASKLYYRVLEAICRAELQNNNVNNLTPLLSNERFHRCLIACSAELVLATHKPVFMMFPAVLDSTGL TAFDLSKIIENFVRHEETLPRQLKRHLNSLEQQLLESMVWEKGSSLYNSLIVARPSVTSEIKRLGLLAEPM PSLDDLVARQNIHVEGLPATPSKKRAAGPDDNADPRSPKRSCNESRNTVVEHNLQTPPPKQSHMVSTSLKA KCHPLQSTFESPTVSNPVGGNEKCADVTIKIFFSKILKLAAVRIRNLCERLQHVELTERVYNVFNQILDQQ TTLFFNRHIDQLILCCLYGVAKVCKLEPSFKELLNNYRKEAQCKPEVFSNVYIGSRNRNDVLVSRHVDIIT FYNEVFVPAAKSFLVSLISSGTRPEDKKNASGQIPGSPKPSPLPNLPDLSPKKVSASHNVYVSPLQQAKMD LLLSPSSRSFYACFGEGTHAYQSPSKDLAAINSCLNYSGRRLSSRLNFDMVSDSVVAGSLGQPNGGSTSLD PAAAFSPISKKREPDT >S.bicolor_isoX2 (SEQ ID NO: 31): MEGVAKPSTTSSGSGVTDGPSSAAASTEERFADLCKSKLGLDESTTRQAMQLFKETKNILKSSVSSLGGGS PEEIERFWSACVLYCVSRLSKAGRSKEDGSVSLCQILRASKLNIVDFFKEMPQFCLKVAHVLTGLYGSDWE KRLELKELQANVVHLSLLSRHYKRAYQELFLSNDGKCSGNSSELINQEASDYYRFGWLLFLALRIQTFSRF KDLVTSTNELVSVLAVLIIHVPVRLRNFDIKDSSYFAKKSDRGVSLIASLCEKYHTSEDELSKALEKTNTL IMDVLKKKPCPATSACQQDNLSFIDPEGLTVFKDFLQGDSLKSSLLILEKEYENAINTKGELDERMFANDE DSLLGSGSLSGGAINLPGTKRKYDVMASPAKSISSPNPMSPPRFCLSPKGSNFCNSKMAPITPVSTAMTTA KWLRSTVSPLPSKPSGELLRFFSACDKDLTDDIAHRAGIILGAIFTSSSFGERICTNMRSANRMDAIWTEQ RKMEALKLYYRVLESMCRAESQILSGNNLTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGIT AFDLSKVIEGFVRHEDTLPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPALSVEINRLGLLAEPMP SLDAIAAHHNISLGGLPPLPFQKQDRLQDKDEVRSPKRACTERRNVLVDSNSFRSPVKDIIKSKLPPPLQS AFASPTRPNPAAGGETCAETGIGVFFSKIAKLAAIRIRSLCERLQLSQQVLERVYSLVQQILSQQTGLFFN RHIDQIILCSIYGVAKISQLELSFKEIIFGYRKQPQCKPQVFRSVYVHWPPRSRNGKTGEDHVDIITFYNE VFIPAVKPLLVEVGPGASPKKKEEEKGSADVGPYPESPRLARFPNLPDMSPKKVSATHNVYVSPLRSSKMD TLLSPSSKSYYACVGESTYAFQSPSKDLKAINNRLNSVSSGKKVNGRLNFDVVSDLVVASSLSSDQNAKPA ATEVVPIKTPVKCEPSDS >S.bicolor_isoX3 (SEQ ID NO: 32): MSSPDPSPATSTPSPATSTQQKQSESLVNLLAEASRFYRRAYNELFSGVTTEQEPESSTNTPEYMLFGWYL FLMLHSRSPELFKDLVSCIHGLVAVLAILLIHVPVKFRSFTVEGSSHLIKQTEKGMDLIASLCHNYHASEE RLKEMMDKSHKAIEDVFGMKALSASECKTENLDMIGTDGLMYFKGLIDMECFQSNLEKMEKLCNSNSSEGK LDFKSILINNDYIPCAENLSGDSTNLGHSKHVFEILASPTKTIKNMLTVPSSPLSPATSGSVKIVQMTPVT SAMTTAKWLREVISSLPEKPSSKLQQLLSSCDRDLTNAVTERVSIVLEAIFPTKSSADGGGSLGLNCANVF DIPWAEARKMEASKLYYRVLEAICRAELQNSNVNNLTPLLLNERFHRCLIACSAELVLATHKPVFMMFPAV LESTGLTAFDLSKIIENFVRHEETLPRQLKRHLNSLEEQLLESMVWEKGSSLYNSLIVARPSIASEIKHLG LLAEPMPSLDDLVARQNIHVEGLPATPSKKRDAGPDDNADPRSPKRSCNESRNTVVEHNLLTPPPKHHMVS TSLKAKCHPLESTFASPTVSNPVGGNEKCADVAIQIFFSKILKLAAVRIRNLCERVQHVELTERVYNVFNQ ILDQQTTLFFNRHIDQLILCCLYGVAKACKVELSFKELLNYYRKEAQCKPEVFQNIYIGSRNSNDVLVSRH VSIISFYNAVFVPAAKPFLVSLISSGTCPEDKKNASGPIPGSPKPSPLPNNLPDMSPKKVSASHNVYVSPL RQTKMDALLSPSSRSFYACIGEGTHAYQSPSKDLAAINSRLNYSGRRINSRLNFDMVSDSVVAGSLGQPNG GSTSFNPAAAFSPLSKKRKPDDQI >T.aestivum_isoX1 (SEQ ID NO: 33): MQLFKETKIILLSSMSSLGSGSPEEIERSWCACVLYCVSKLGNAGKAKEDRGITLRQILRAFDLKIVDFFK EMPQFCIKVGFILTGLYGSDWEKRLELQELQANLVHLCSLGRHYRRAYQELFLLNDGKPANNSSELNVQQA SEYYDFGWLLFLVLRNQASSAVKNLLTSTTELVSVLAVLIIHIPVRLRNFSIEDSSCFAKKSDKGVNLIAS LCERYLTSEDELSKALQKTNILIKDILKKKPCSDVSECQQGSLSFIDPEGLTFFKNFLEEDSLKSSLQVLE KEYVNGLDTKGELDARMFANEEDSLLGSGSLSGGALKLPGTKRKYDDVMASPTKSTASRAPMSPPRFCPSP NGNSFCNSKMAPFTPVSTAMTTAKWLRSTISPLPSKPSGELLRFFSACDKDVTDDITCRAAIILGAIFTGS SFGERMCTSLRNTSGMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGSNLTSLLSNERFHRCMIACSAE LVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEDSLPRELKRHLNSLEERLLESMAWEKGSSMYNS LIVARPTLSAEINRLGLLAEPMPSLDAIAVHHDISLGGLPPLPFHKQPDKDEVRSPKRACTERRNVLVDNS FRSPVKDAIKSKFLPPLQSAFASPTRPNPAAGGETCAETGIGVFLSKITKLAAIRIKCLCERLQLSQQILE RVYSLVQQIISQQTALFFNRHIDQIILCCIYGVAKISQLALTFKEIIFSYRKQSQCKPQVFRSVYVNWPSR SRSGKIGEDHVDIITFYNEVFIPTVKPLLVDLGPGTSPNRNNEPKSGGDAASFPESPRLSRFPNLPDMSPK KVSATHNVYVSPLRSSKMDTLLSPSSKSYYACVGESTHAFQSPSKDLNAINTRLNSGKKVNGRLNFDVVSD LVVARSLSDQNGSSAAAMAVFGTKTPVKGEQQDP >T.aestivum_isoX2 (SEQ ID NO: 34): MERQPPPPPAVETHFADLCKELEVDEGVASEAAALLEEGKGVFLTSPSFGSKSPEDVERLCFAFVLYCVAK LKGKGMKQESSRFRLWKILEGCKLKYNDFFKESQQLLSKIDHVLRGRYGTDWEDQLELKQLQSLVNLLADA SRFYCKAFNELFLSASTGQEPGSTKSNPEYFCFGWLIFLALRSKSPELFKDLVSCIHGLVAILAILLIHVP AKFRSFTIEGSSHLIKQTVRGVDLLASLCHNYHTSEDRLREMMGKFHKAIEEFFSTKAVRASECKTETLDK IDTDGLIYFRDLLDKECFHSNFEKLEKLSSTTSWEGELDLKRFLINNDNIISAENSSRDFTNLSCPKRVFE TLASPTKTIKNMLTVPSSPSSPANGGSVKVVQMTPVTSAMTTAKWLREVISLLPEKPSSKLEKFLSSCDTD LTSDVTKRVSIILEVIFPTKPSGHWSGSMGLNCTNAFDIPWAQARKMEASKLYYRVLEAICRAESLNTNVN NLTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEESLPRELKRHLN SLEEQLLESMAWEKGSSLYNSLVVARPSLASEINRLGLLAESMPSLDDIVARQNFHAEDLPATPSKKRAAY SDENGDSRSPKRLCIEPRSTLVDQISQTPPAKQSYTLKAKWRPLQSTFASPTVSNPVGGNEKCAEVGVHIF FSKILKLAAIRIRNLCERLRHVEQTERVYNIFKQILDQQTTLFFNRHIDQLILCSLYGVAKVSQLTLTFKE IVNNYKREQQCIPEVFRSVFVVNTNHNGGLGSRHVDIIVFYNEVFVPAIKPFLVALIPSGGAHPDNKKNPN SQIPGSPKSPPFSNLPDMSPKKVSSSHNVYISPLRQTKKDALLSPSSRSFYACIGESTHAYQSPSEDLAAI NNCLNYTSRRINTRINFDMVSDSVVAGSLGQPNGVPASSDPAGSFSFLSRKDNSGPDS >T.aestivum_isoX3 (SEQ ID NO: 35): MEPQPPPTPAVETHFADLCKELEVDEGVAGEAAALLEEGKGVFLTPPSFGSKSPEDVERLCFAFVLYCVAK LKGKGMKEESSRVRLWKILEGCKLKYNDFFKESQQLLSKIDHVLRSWYGTDWEDQLELKQLQSLVNLLADA SRFYCKAFNELFLNASTGQEPGSTKSNPEYFCFGWLFFLALRSKSPELFKDLVSCXXXXXXXXAILLIHVP AKFRSFTIEGSSHSIKQTVRGVDLLASLCHNYHTSEDRLKEMMGKFHKAIEVFFSTKAVRASECKIETLDK IDTDGLIYFRDLLDKECFHSNFEKLEKLSSTTSWEGELDLKRFLINNDNIISAENSSRDFTNLSCPKRVFE TLASPTKTIKNMLTVPSSPSSPANGGSVKVVQMTPVTSAMTTAKWLREVISLLPEKPSSKLEEFLSSCDTD LTSDVTKRVSIILEAIFPTKPSGHWAGSMGLNCTNAFDIPWAEARKMEASKLYYRVLEAICRAESLNTNVN NLTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEESLPRELKRHLN SLEEQLLESMAWEKGSSLYNSLVVARPSLAPEINRLGLLVESMPSLDDIVARQNFHAEDLPATPSKKRAAD SDENGDSRSPKRLCIEPRSTLVDQISQTPPAKQSYTLKAKWRPLQSTFASPTVSNLVGGNEKCAEVGVHIF FSKILKLAAIRIRNLCERLRHVEQTERVYNIFKQILDQQTTLFFNRHIDQLILCSLYGVAKVSQLTLTFKE IVNNYRREQQCIPEVFRSVFVVNTNHNGGLGSHHVDIIVFYNEVFVPAVKPFLVALIPSGGAHPDNKKNPN SQISGSPKPSPFSNLPDMSPKKVSSSHNVYISPLRQTKKDALLSPSSRSFYACIGESTHAYQSPSEDLAAI NNCLNYTSRRINTRINFDMVSDSVVAGSLGQPNGVPASSDPAGSFSFLSRKDNPGPDS >T.aestivum_isoX4 (SEQ ID NO: 36): MEPQPPPPPAVETHFADLCKELEVDEGVAGEAAALLEEGKDVFLTSPSFGSKSPEDVERLCFAFVLYCVAK LKGKGMKEESSRVRLWKILEGCKLKYNDFFKESQQLLSKIDHVLRSRYGTDWEDQLELKQLQSLVNLLADA SRFYCKAFNELFLSASTGQEPGSTKSNPEYFCFGWLFFLALRSKSPELYKDLVSCIHGLVAILAILLIHVP AKFRSFTIEGSSHLIKQTVRGVDLLASLCHNYHTSEDRLKEMMGKFHKAIEVFFSTKAVRASECKLETLDK IDTDGLIYFRDLLDKECFHSNFEKLEKLSSTTSWEGELDLKRFLINNDNIISAENSSRDFTNLSCPKRVFE TLASPTKTIKNMLTVPSSPSSPVNGGSVKVVQMTPVTSAMTTAKWLRGVISSLPEKPSSKLEKFLSSCDTD LTSDVTKRVSIILEAIFPTKPSGHWGGSMGLNCTNAFDIPWAEARKMEASKLYYRVLEAICRAESLNTNVN NLTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEESLPRELKRHLN SLEEQLLESMAWEKGSSLYNSLVVARPSLASEINRLGLLAESMPSLDDIVARQNFHAEDLPATPSKKRAAD SDENGDSRSPKRLCIEPRSTLVDQISQTPPAKQSYTLKAKWRPLQSTFASPTVSNLVGGNEKCAEVGVHIF FSKILKLAAIRIRNLCERLRHVEQTEHVYNIFKQILDQQTTLFFNRHIDQLILCSLYGVAKVSQLTLTFKE IVNNYKREQQCIPEVFRSVFVVNTNHNGGLGSRHVDIIVFYNEVFVPAVKPFLVALIPSGGAHPDNKKNPN SQIPGSPKSPPFSNLPDMSPKKVSSSHNVYISPLRQTKKDALLSPSSRSFYACIGESTHAYQSPSEDLAAV NNCLNYTSRRINTRINFDMVSDSVVAGSLGQPHGVPASSDPAGSFSFLSRKDNPGPDS >T.aestivum_isoX5 (SEQ ID NO: 37): MDGADAPQPARSGSGVTACSTSTAPARMNGGANANNNAAAAMEERFADVCKRKLVLDESTTRQAMQLFKET KIILLSSMSSLGSGSPEEIERSWCACVLYCVSKLGNAGKAKEDRGITLRQILRAFDLKIVDFFKEMPQFCI KVGFILTGLYGSDWEKRLELQELQANLVHLCSLGRHYRRAYQELFLLNDGKPANNSSELNVQQASEYYDFG WLLFLVLRNQASSAVKNLLTSTTELVSVLAVLIIHIPVRLRNFSIEDSSCFAKKSDKGVNLIASLCERYLT SEDELSKALQKTNILIKDILKKKPCSDVSECQQGSLSFIDPEGLTFFKNFLEEDSLKSSLQVLEKEYVNGL DTKGELDARMFANDEDSLLGSGSLSGGALKLPGTKRKYDDVMASPTKSTASRAPMSPPRFCPSPNGNSFCN SKMAPFTPVSTAMTTAKWLRSTISPLPSKPSGELLRFFSACDKDVTDDITCRAAIILGAIFTGSSFGERMC TSLRNTSGMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGSNLTSLLSNERFHRCMIACSAELVLATHK TVTMMFPAVLEKTGITAFDLSKVIESFVRHEDSLPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPT LSAEINRLGLLAEPMPSLDAIAVHHDISLGGLPPLPFHKQPDKDEVRSPKRACTERRNVLVDNSFRSPVKD AIKSKFLPPLQSAFASPTRPNPAAGGETCAETGIGVFLSKITKLAAIRIKCLCERLQLSQQILERVYSLVQ QIISQQTALFFNRHIDQIILCCIYGVAKISQLALTFKEIIFSYRKQSQCKPQVFRSVYVNWPSRSRSGKIG EDHVDIITFYNEVFIPTVKPLLVDLGPGTSPNRNNEPKSGGDAASFPESPRLSRFPNLPDMSPKKVSATHN VYVSPLRSSKMDTLLSPSSKSYYACVGESTHAFQSPSKDLNAINTRLNSGKKVNGRLNFDVVSDLVVARSL SDQNGSSAAAMAVFGTKTPVKGEQQDP >T.aestivum_isoX6 (SEQ ID NO: 38): MDGADAPQPARSGSGVTACSTSTALGRMNGGANASNNAAAAMEERFADVCKRKLVLDESTTRQAMQLFKET KIILLSSMSSLGSGSPEEIERSWCACVLYCVSKLGNAGKAKEDRGITLRQILRAFDLKIVDFFKEMPQFCI KVGFILTGLYGSDWEKRLELQELQANLVHLCSLGRHYRRAYQELFLLNDGKPANNSSELNVQQASEYYDFG WLLFLVLRNQASSAVKNLLTSTTELVSVLAVLIIHIPVRLRNFSIEDSSCFAKKSDKGVNLIASLCERYLT SEDELSKALQKTNILIKDILKKKPCSDVSECQQGSLSFIDPEGLTFFKNFLEEDSLKSSLQVLEKEYVNGL DTKGELDARMFANDEDSLLGSGSLSGGALKLPGTKRKYDDVMASPTKSTASRAPMSPPRFCPSPNGNSFCN SKMAPFTPVSTAMTTAKWLRSTISPLPSKPSGELLRFFSACDKDVTDDIICRAAIILGAIFTGSSFGERMC TSLRNTSGMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGSNLTSLLSNERFHRCMIACSAELVLATHK TVTMMFPAVLEKTGITAFDLSKVIESFVRHEDSLPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPT LSAEINRLGLLAEPMPSLDAIAVHHDISLGGLPPLPFHKQPDKDEVRSPKRACTERRNVLVDNSFRSPVKD VIKSKFLPPLQSAFASPTRPNPAAGGETCAETGIGVFLSKITKLAAIRIKCLCERLQLSQQILERVYSLVQ QIISQQTALFFNRHIDQIILCCIYGVAKISQLALTFKEIIFSYRKQSQCKPQVFRSVYVNWPSRSRSGKIG EDHVDIITFYNEVFIPTVKPLLVDLGPGTSPNRNNEPKSGGDAAPFPESPRLSRFPNLPDMSPKKVSATHN VYVSPLRSSKMDTLLSPSSKSYYACVGESTHAFQSPSKDLNAINTRLNSGKKVNGRLNFDVVSDLVVARSL SDQNGGSAAAMAVFGTKTPVKGEQQDP >T.aestivum_isoX7 (SEQ ID NO: 39): MDGADAPQPARSGSGVTACSTSTAQGRMNGGANASNNAAAAMEERFADVCKRKLVLDESTTRQAMQLFKET KIILLSSMSSLGSGSPEEIERSWCACVLYCVSKLGNAGKAKEDRGITLRQILRAFDLKIVDFFKEMPQFCI KVGFILTGLYGSDWEKRLELQELQANLVHLCSLGRHYRRAYQELFLLNDGKPANNSSELNVQQASEYYDFG WLLFLVLRNQASSAVKNLLTSTTELVSVLAVLIIHIPVRLRNFSIEDSSCFAKKSDKGVNLIASLCERYLT SEDELSKALQKTNILIKGILKKKPCSDVSECQQGSLSFIDPEGLTFFKNFLEEDSLKSSLQVLEKEYVNGL DTKGELDARMFANEEDSLLGSGSLSGGALKLPGTKRKYDDVMASPTKSTASRAPMSPPRFCPSPNGNSFCN SKMAPFTPVSTAMTTAKWLRSAISPLPSKPSGELLRFFSACDKDVTDDITCRAAIILGAIFTGSSFGERMC TSLRNTSGMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGSNLTSLLSNERFHRCMIACSAELVLATHK TVTMMFPAVLEKTGITAFDLSKVIESFVRHEDSLPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPT LSAEINRLGLLAEPMPSLDAIAVHHDISLGGLPPLPFHKQPDKDEVRSPKRACTERRNVLVDNSFRSPVKD VIKSKFLPPLQSAFASPTRPNPAAGGETCAETGIGVFLSKITKLAAIRIKCLCERLQLSQQILERVYSLVQ QIISQQTALFFNRHIDQIILCCIYGVAKISQLALTFKEIIFSYRKQSQCKPQVFRSVYVNWPSRSRSGKIG EDHVDIITFYNEVFIPTVKPLLVDLGPGTSPNRNNEPKSGGDVFVFFVTSAAPFPESPRLSRFPNLPDMSP KKVSATHNVYVSPLRSSKMDTLLSPSSKSYYACVGESTHAFQSPSKDLNAINTRLNSGKKVNGRLNFDVVS DLVVARSLSDQNGGSAAAMAVFGTRTPVKGEQQDP >V.vinifera (SEQ ID NO: 40): MDGVKPVASAEQGGAVEARFTEFCKNGLQLDESTFAQAMKLFEESNHLLSTTSLSAIGNGPEDSERYWFAF ILYSVKRLSEGTAENVQQGNDENGFNLCQILRVSKLNIVDFFKELPQFIVKVGPILGNLYGPDWEKRLEAK ELQANFVHLSILSKYYKRAYLEFFSTSGSNLDKQSSVISASGYVSDYHRFGWLLFLALRVHAFSRFKDLVT CTNGLVSILAILILHVPISFRSFTINNYPRFVKKGNKGMDLLASLCDIYETSEDEVRKTMEQTNKVIVDIL KKKPCLASECKSENLASIDPDGLVYFEDLMDESSLSSSINILEKDYDAAIRNKGELDERVFINGEDSLLGS GSLSGGAMSISGAKRKIDSLASPAKTITSPLSPNRSPGILGGANSKMAPTPVTTAMTTAKWLRTVISPLPS KPSAELERFLTSCDKDVTSDVIRRANVILEAIFPSIAFGERCVTGSASLMDSIWAQQRRMEAMKLYYRVLE AMCTAEAQVLHANNLTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAFDLSKVIESFIRH EESLPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARAALSAEVNRLGLLAEPMPSLDAISMHINASCG GLPPVPSLQKRESSPGQNGDIRSPKRVCPDYRSVLVERNSFTSPVKDRFLALNNLKSKLPPPPLQSAFASP TRPNPGREGETCAETGINIFFSKIIKLAAVRINGMVERLQLSQQMRENVYCLFQQILNRRTSLFFNRHIDQ IILCCFYGLAKISQMNLTFREIIHNYRKQPQCKPQIFRSVFVDWSSARRNGKTGKEHVDIITFYNEIFIPA VKPLLMEIGPGGGTTTKTNRVPEVNNNNDAQCPESPKISPFPSLPDMSPKKVSAAHNVYVSPLRSSKMDAL ISHSSKSYYACVGESTHAYQSPSKDLTAINNRLNSTRKLRGALNFDDVEGLVSDSLVAKSLYLQNGSCASS SGAPLKLEQPDT >Z.mays_isoX1 (SEQ ID NO: 41): MSSLDPSPATSTQQQKQLESLVNLLTQGSRFYRKAYNELFSGVTTEQDPDSSTNIPEYMLFGWHLFLMLHL RSPELFKDLVSCIHGLVAVLAILLIHVPAKFRSFTIEGSSHLIKQTEKGVDLIASLCHNYHTSEERLKEML HKSHNAIEDIFHMKALSASECKPENLDKIDTDDLMYFKGLIDMECFQSNLEKMEKLCNSNSCKGELDFKSI LINNDYIPYDENSTGDSTNLGHSKCAFETLASPTKTIKNMLTVPSSPLSPATGGSVKIVQMTPVTSAMTTA KWLREVISSLPDKPSSKLQQFLSSCDRDLTNAVTERVSIVLEAIFPTKSSANRGVSLGLNCANAFDIPWAE ARKVEASKLYYRVLEAICRAELQNSNVNNLTPLLSNERFHRCLIACSADLVLATHKTVIMMFPAVLESTGL TAFDLSKIIENFVRHEETLPRELKRHLNSLEEQLLESMAWEKGSSLYNSLIVARPSVASEINRLGLLAEPM PSLDDLVSRQNVRIEGLPATPSKKRAAGPDDNADPRSPKRSCNESRNTVVERNLQTPPPKQSHMVSTSLKA KCHPLQSTFASPTVCNPVGGNEKCADVTIHIFFSKILKLAAIRIRNLCERVQCVEQTERVYNVFKQILEQQ TTLFFNRHIDQLILCCLYGVAKVCQLELTFREILNNYKREAQCKPEVFSSIYIGSTNRNGVLVSRHVGIIT FYNEVFVPAAKPFLVSLISSGTHPEDKKNASGQIPGSPKPSPFPNLPDMSPKKVSASHNVYVSPLRQTKLD LLLSPSSRSFYACIGEGTHAYQSPSKDLAAINSRLNYNGRKVNSRLNFDMVSDSVVAGSLGQINGGSTSDP AAAFSPLSKKRETDT >Z.mays_isoX2 (SEQ ID NO: 42): MSSQDPPPATSTQKKQSESLVNLLAEASRFYRKAYNELFSGLITEWEPESSTNIPDYMLFGWHLFLNLRLR SPELFKDLVSCIHGLVAVLAILLVHVPAKFRTFTIEGSSHLIKQTEKGVDLIASLCHNYHTSEECLKEMMD KSHKAIEEVFSMKALSASECKTENLDKIDTDRLMYFKGLIDMECFQSNLEKIEKLCNSNNCEAELDFKLIL TNNDYIPCAENLSRDSTNLGCSKCAFETLASPRKTIKNMLTVPSSPLSPTNGCSVKIVQMTPITSAMTTAK WLREVISSLPEKPSSKLQQLMSSCDRDLTYAVTERVSIVLEAIFPTKSSADRGGSLGLNCANAFDTLWADA RKMEASKLYYRVLEAICRAELQNSNVNNLTPLLSNERFHRCLIACSADVVLATHKTVIMMFPAVLESAGLT SFDLSKIIENFVRHEETLPRELKRHLNSLEEQILESMAWEKGSSLYNSLIVARPSVASEINRFGLLAESMP SLDDLVARQNIHIEGLPATPSKKRAAGRDDNADPRSPKRPCNESRSPVVEHNLQTPPPKQCHMVLTSLKAK CHPLQSTFASPTVSNPVGGNEKCADVTIQIFFSKILKLAAIRIRNLCERIQYMEQTERVYNVFKQILDQQT TLFFNRHIDQLILCCLYGVAKVCQLELSFREILNNYKKEAQCKPEVFLSIYIGSRNHNGVLISRHVDIITF YNEVFVPAAKPFLVSLISSGTRPEDKKNASGQVPGSPKLSPFPNLPDMSPKKVSASHNVYVSPLRQTKMDL LLSPSSRSFYACIGEGTHAYQSPSKDLAAINSRLNYNGRRVNSRLNFDMVSDSVVAGSLGQPNGGSTSLDP AAAFSPLSKRKPDT >Z.mays_isoX3 (SEQ ID NO: 43): MSSLDPSPATSTQQKQLESLVNLLTQGSRFYRKAYNELFSGVTTEQDPDSSTNIPEYMLFGWHLFLMLHLR SPELFKDLVSCIHGLVAVLAILLIHVPAKFRSFTIEGSSHLIKQTEKGVDLIASLCHNYHTSEERLKEMLH KSHNAIEDIFHMKALSASECKPENLDKIDTGMEPFLTYDLMYFKGLIDMECFQSNLEKMEKLCNSNSCKGE LDFKSILINNDYIPYDENSTGDSTNLGHSKCAFETLASPTKTIKNMLTVPSSPLSPATGGSVKIVQMTPVT SAMTTAKWLREVISSLPDKPSSKLQQFLSSCDRDLTNAVTERVSIVLEAIFPTKSSANRGVSLGLNCANAF DIPWAEARKVEASKLYYRVLEAICRAELQNSNVNNLTPLLSNERFHRCLIACSADLVLATHKTVIMMFPAV LESTGLTAFDLSKIIENFVRHEETLPRELKRHLNSLEEQLLESMAWEKGSSLYNSLIVARPSVASEINRLG LLAEPMPSLDDLVSRQNVRIEGLPATPSKKRAAGPDDNADPRSPKRSCNESRNTVVERNLQTPPPKQSHMV STSLKAKCHPLQSTFASPTVCNPVGGNEKCADVTIHIFFSKILKLAAIRIRNLCERVQCVEQTERVYNVFK QILEQQTTLFFNRHIDQLILCCLYGVAKVCQLELTFREILNNYKREAQCKPEVFSSIYIGSTNRNGVLVSR HVGIITFYNEVFVPAAKPFLVSLISSGTHPEDKKNASGQIPGSPKPSPFPNLPDMSPKKVSASHNVYVSPL RQTKLDLLLSPSSRSFYACIGEGTHAYQSPSKDLAAINSRLNYNGRKVNSRLNFDMVSDSVVAGSLGQING GSTSDPAAAFSPLSKKRETDT >Z.mays_isoX4 (SEQ ID NO: 44): MEGFAKPSTSSGSGVTVRASVAAASIEERFADLCKAKLGLDESTTRQAMQLLKETNNILKSSMSSLGGGSP EEIERFWSACVLYCVSRLSKAGRSKEDGSVSLCQILRASKLNIVDFFKEMPQFCIKVAHILTGLYGSDWEK RLELKELQANVVHLSLLSSYYKRAYQELFLSNDGKSSDNSSESNNQEASDYYRFGWLLFLVLRIQTFSRFK DLVTSTNELVSVLAVLIIHVPVRLRNFDIKDSSYFGKKSDRGVSLIASLCEKHHTSEDELSKALEKTNTLI MDILKKKPCPATSACQQDNLSFIDPEGLTVFKDLLQGDSLKPSLIILEKEYENAINTKGELDERMFANDED SLLGSGSLSGGAINLPGTKRKYDVMASPAKSISSPNPMSPPRFCLSPKGNGFCNSKMAPSTPVSTAMTTAK WLRNTVSPLPSRPSGELLRFFSACDKDLTDDIAHRAGIILGAIFTSSSFGERICTSMRSASRMDAIWTEQR KMEALKLYYRVLESMCRAESQILSGNNLTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITA FDLSKVIEGFVRHEDTLPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPALSVEISRLGLLAEPMPS LDAIAAHHNISLGGLPPLPFQKQERLQDKDEVRSPKRACTERRNVLVDSNSLRSPVKDIIKPKLPPLQSAF ASPTRPNPAAGGETCAETGIGVFFSKISKLAAIRIRSLCERLQLPQQVLERVYSLVQQILSQQTGLFFNRH IDQIILCSIYGVAKISQLELSFKEIIFGYRKQPQCKPQVFRSVYVHWPPRSRNGKTGEDHVDIITFYNEVF IPAVKSLLVEVGPGASASPKKKEEEKGPADVGPFPESPRLARFPNLPDMSPKKVSATHNVYVSPLRSSKMD TLLSPSSKSYYACVGESTYAFQSPSKDLKAINNRLNSVSGGKKVSGRLNFDVVSDLVVASSLGSDRDAKPA ADPAKTTPVKCEPSDS >Z.mays_isoX5 (SEQ ID NO: 45): MSSQDPPPATSTQKGGTDLVLFLHWYLDLRIQQKQSESLVNLLAEASRFYRKAYNELFSGLITEWEPESST NTPDYMLFGWHLFLTLRSRSPELFKDLVSCIHGLVAVLAILLVHVPAKFRTFTIEGSSHLIKQTEKGVDLI ASLCHNYHTSEECLKEMMDKSHKAIEEVFSMKALSASECKTENLDKIDTGMEPFLAYRLMYFKGLIDMECF QSNLEKMEKLCNSNNCEAELDFKLILTNNDYIPCAENLSRDSTNLGCSKCAFETLASPTKTIKNMLTVPSS PLSPTNGGSVKIVQMTPITSAMTTAKWLREVISSLPEKPSSKLQQLMSSCDRDLTNAVTERVSIVLEAIFP TKSSADRGGSLGLNCANAFDTLWADARKMEASKLYYRVLEAICRAELQNSNVNNLTPLLSNERFHRCLIAC SAELVLATHKTVIMMFPAVLESTGLTSFDLSKIIENFVRHEETLPRELKRHLNSLEEQILESMAWEKGSSL YNSLIVARPSVASEINRFGLLAESMPSLDDLVARQNIHIEGLPATPSKKRAAGRDDNADPRSPKRPCNESR STVVEHNLQTPPPKQCHMVLTSLKAKCHPLQSTFASPTVSNPVGGNEKCADVTIQIFFSKILKLAAIRIRN LCERIQYMEQTERVYNVFKQILDQQTTLFFNRHIDQLILCCLYGVAKVCQLELSFREILNNYKKEAQCKPE VFLSIYIGSRNHNGVLISRHVDIITFYNEVFVPAAKPFLVSLISSGTRPEDKKNASGQVPGSPKLSPFPNL PDMSPKKVSASHNVYVSPLRQTKINMAYACLNSTAQYVSTTASMTKLLSPKLGMDLLLSPSSRSFYACIGE GTHAYQSPSKDLAAINSRLNYNGRRVNSRLNFDMVSDSVVAGSLGQPNGGSTSLDPAAAFSPLSKRKPDT > Z.mays_isoX6 (SEQ ID NO: 46): MDGVAKPSTSSGSGVTDSASAAAASIDERFADLCESKLGLDDSMTRQAMQLFNETKNIKSSMSSLGGGSPE EVERFWSACVMYCVSRLSKARRSKESGSVSLCQILRASKLNIVDFFKEMPQFCIKVAHILTGLYGSDWEKR LELKELQANVVHLSLLSRHYKRAYQELFLPNDGKCSDSSSESNNQEVSDYYRFGWLLFLVLRIQTFSRFKD LVTSTNELVSVLAVLIIHIPVRLRNFDIKDSSYFGKKSDRGVSLIASLCEKYHTSEDELSKALEKTNTLIL DILKKKPWTATSACQQDNLTFIDPEGLTVFKDLLQGGSLKSSLLILEKEYENAINTRGELDERMFANDEDS LLGSGSLSGGAINLPGTKRKYDVMASPAKSISSPNPMSPPRFCLSPKGNSFCNSKMAPITPVSTAMTTAKW LRSTVSPLPSKPSGELLRFFSACDKDVTDDIVHRAGIILGAIFTSSSFGEQICTNMRSANRMDAIWTEQRK MEALKLYYRVLESMCRAESQILSGNNLTLLLSNERFHRCMIACSAELVLATHKTVTLMFPAVLEKTGITAF DLSKVIEGFVRHEDTLPRELKRHLNSLEERLLESMSWEKGSSMYNSLIVARPVLSIEINRLGLLAEPMPSL DAIAAHHNISMGGLPPLPFQKQERLQDKDEIRSPKRACTERRHVPVDSNSFRSQVKDSIKLKLPPPLQSAF ASPTRPNPAAGGETCAETGIAVFFSKISKLAAIRIRSLCERLQLSQQVLERVYSLVQQILSQQTGLFFNRH IDQIILCSIYGVAKISQLQLSFKEIVFGYRKQPQCKPQVFRSVYVHWPPTSHNGKTGEDHVDIITFYNEVF IPAVKPLLVEVGPGASPKRKEEGKGSVYVGPYPESPRLARFPNLPDMSPKKVSATHNVYVSPLRSSKMDTL LSPSPKSYYACVGESTYAFQSPSKDLKAINNRLNSWKKVSGRLNFDIVSDLVVASSLNGVQNAKPAATEVV PTKTPVKCEPSES Alignments: The alignments depicted below show alignment of SEQ ID NO 7-46 to one another. S.bicolor_isoX1 --MSSPDPSPS---------------------------------------TST------- S.bicolor_isoX3 --MSSPDPSPA---------------------------------------TST------- Z.mays_isoX1 --MSSLDPSPA---------------------------------------TST------- Z.mays_isoX3 --MSSLDPSPA---------------------------------------TST------- Z.mays_isoX2 --MSSQDPPPA---------------------------------------TST-------Z.mays_isoX5 --MSSQDPPPA---------------------------------------TST-------O.sativa_isoX2 --MAS-QPPAA------------------------------------------------- O.vulgare_isoX1 ---MEPQPPPP---------------------------------------PAV------- T.aestivum_isoX3 ---MEPQPPPT---------------------------------------PAV------- T.aestivum_isoX2 ---MERQPPPP---------------------------------------PAV-------T.aestivum_isoX4 --ME-PQPPPP---------------------------------------PAV-------O.vulgare_isoX2 --FPPAPPPRPFSSPPRRPTATTLLPARRSPDSVRPRSSYWGRQPMDGADAPQPARSGSG T.aestivum_isoX1 ------------------------------------------------------------ T.aestivum_isoX5 --MDGADAPQP---------------------------------------ARSGSGVTAC T.aestivum_isoX7 --MDGADAPQP---------------------------------------ARSGSGVTACT.aestivum_isoX6 --MDGADAPQP---------------------------------------ARSGSGVTACO.sativa_isoX1 --MEGAAPPAS---------------------------------------SGS------- Z.mays_isoX6 --MDGVAKPST---------------------------------------SSG------- S.bicolor_isoX2 --MEGVAKPST---------------------------------------TSS------- Z.mays_isoX4 --MEGFAKPST---------------------------------------SSG-------A.thaliana --MEEVQPPVT---------------------------------------PPI-------B.napus_isoX4 --MEEVQPPVT---------------------------------------PPI------- B.rapa_isoX3 --MEEVQPPVT---------------------------------------PPI------- B.rapa_isoX2 --MEEVQPPVT---------------------------------------PPI------- B.rapa_isoX4 --MEEVQPPVT---------------------------------------PPI-------B.napus_isoX3 --MEEVQPPVT---------------------------------------PPI-------B.oleracea_isoX2 --MEEVQPPVT---------------------------------------PPI------- B.oleracea_isoX1 --MEEVQPPVT---------------------------------------PPI------- B.rapa_isoX1 --MEEVQPPVT---------------------------------------PPI------- B.napus_isoX2 --MEEVQPPVT---------------------------------------PPI-------B.napus_isoX1 --MEEVQPPVT---------------------------------------PPI-------S.tuberosum MNMEELKNYSN---------------------------------------SEE------- S.pennellii MNMEELKNHSN---------------------------------------SEE------- S.lycopersicum MNMEELKNHSN---------------------------------------SEE------- N.bentamiana --MVELNNCSN---------------------------------------SEE-------N.tabacum --MVELNNCSN---------------------------------------SEE-------V.vinifera --MDGVKPVAS---------------------------------------AEQ------- G.max_isoX3 --MEDGKP---------------------------------------------------- G.max_isoX1 --MS---PPPA---------------------------------------ESN------- G.max_isoX2 --MS---PPPA---------------------------------------ESN------- S.bicolor_isoX1 ------------------------------------------------------------ S.bicolor_isoX3 ------------------------------------------------------------ Z.mays_isoX1 ------------------------------------------------------------ Z.mays_isoX3 ------------------------------------------------------------ Z.mays_isoX2 ------------------------------------------------------------ Z.mays_isoX5 ------------------------------------------------------------ O.sativa_isoX2 ----------------------VEARLADLCKE-LGVDEGVAGEAAAVLEEGKGALL--A O.vulgare_isoX1 -----------------------ETHFADLCKE-LGVDEGVASEAAALLEEGKGVFL--T T.aestivum_isoX3 -----------------------ETHFADLCKE-LEVDEGVAGEAAALLEEGKGVFL--T T.aestivum_isoX2 -----------------------ETHFADLCKE-LEVDEGVASEAAALLEEGKGVFL--T T.aestivum_isoX4 -----------------------ETHFADLCKE-LEVDEGVAGEAAALLEEGKDVFL--T O.vulgare_isoX2 VTASTAPGRMNGGASANANAAAMEERFADVCKRKLVLDESTTRQAMQLFKETKIILL-SS T.aestivum_isoX1 ---------------------------------------------MQLFKETKIILL-SS T.aestivum_isoX5 STSTAPARMNGGANANNNAAAAMEERFADVCKRKLVLDESTTRQAMQLFKETKIILL-SS T.aestivum_isoX7 STSTAQGRMNGGANASNNAAAAMEERFADVCKRKLVLDESTTRQAMQLFKETKIILL-SS T.aestivum_isoX6 STSTALGRMNGGANASNNAAAAMEERFADVCKRKLVLDESTTRQAMQLFKETKIILL-SS O.sativa_isoX1 -----EVTGAGSGKVDAGGGAAMEERFADLCKSKLGLDESITRQAMQLFKESKSILL-SS Z.mays_isoX6 ---------SGVTDSASAAAASIDERFADLCESKLGLDDSMTRQAMQLFNETKNIKS--S S.bicolor_isoX2 --------GSGVTDGPSSAAASTEERFADLCKSKLGLDESTTRQAMQLFKETKNILK-SS Z.mays_isoX4 ---------SGVTVRASVAAASIEERFADLCKAKLGLDESTTRQAMQLLKETNNILK-SS A.thaliana ----------------EPNGKRSEASLLDICEKVLSLDGSTCDEALKLFTETKRILS-AS B.napus_isoX4 ----------------DPNGKRSEATLLDLCEKVLSLEGSVCDEVLKLFTETKRILS-AN B.rapa_isoX3 ----------------DPNGKRSEATLLDLCEKVLSLEGSVCDEALKLFTETKRILS-AN B.rapa_isoX2 ----------------DPNGKRSEATLLDLCEKVLSLEGSVCDEALKLFTETKRILS-AN B.rapa_isoX4 ----------------DPNGKRSEATLLDLCEKVLSLEGSVCDEALKLFTETKRILS-AN B.napus_isoX3 ----------------DPNGKRSEAVLLELCEKVLSLEGSICDEALKLFTETKPILS-AN B.oleracea_isoX2 ----------------DPNGKRSEAILSDLCEKVLSLEGSSCDEALKLFTETKPILS-AN B.oleracea_isoX1 ----------------DPNGKRSEAILSDLCEKVLSLEGSSCDEALKLFTETKPILS-AN B.rapa_isoX1 ----------------DPNGKRSEAVLLELCEKVLSLEGSICDEALKLFTETKPILS-AN B.napus_isoX2 ----------------DPNGKRSEAVLLELCEKVLSLEGSICDEALKLFTETKPILS-AN B.napus_isoX1 ----------------DPNGKRSEAVLLELCEKVLSLEGSICDEALKLFTETKPILS-AN S.tuberosum ----------------NGSVDSLEVRFTDFCKNGLSMGESFMVEARKLFMDSKHLLL-SN S.pennellii ----------------NGSVDSLEVRFTDFCKNGLSMGESFMVEARKLFMDSKHLLL-SN S.lycopersicum ----------------NGSVDSLEVRFTDFCKNGLSMGESFMVEARKLFMDSKHLLL-SN N.bentamiana ----------------TGGVDSLEVRFTDFCKNGLSMGESFVTEATKLFNDSKHLLL-SN N.tabacum ----------------NGCVDSLEVRFTDFCKNGLSMGESFVTEATKLFNDSKHLLL-SN V.vinifera -------------------GGAVEARFTEFCKNGLQLDESTFAQAMKLFEESNHLLSTTS G.max_isoX3 ----------------MNGGDQVESRFVEFCKNGLALEEKSCKKAKNLFGETKHILL-SN G.max_isoX1 ---------AMEDGKSENGGDQVESRFFEFCKNGLALEEKSCKEAANLFGETKHILL-SN G.max_isoX2 ---------AMEDGKPENGGDQVESRFFEFCKNGLALEEKSCKEATNLFGETKHILL-SN S.bicolor_isoX1 ------------------------------------------------------------ S.bicolor_isoX3 ------------------------------------------------------------ Z.mays_isoX1 ------------------------------------------------------------ Z.mays_isoX3 ------------------------------------------------------------ Z.mays_isoX2 ------------------------------------------------------------ Z.mays_isoX5 ---QKGGTDLVLFLHWYLDL---------------------------------------- O.sativa_isoX2 SPSFGSKSPEDAEKLCFAFVLYCVSKLK-------ETKAGSSGVRLWEILKGCKLKYDDF O.vulgare_isoX1 PPLFGSKSPEDVERLCFAFVLYCVAKLK-----GKGMKEESSRVRLWKILEGCKLNYNDF T.aestivum_isoX3 PPSFGSKSPEDVERLCFAFVLYCVAKLK-----GKGMKEESSRVRLWKILEGCKLKYNDF T.aestivum_isoX2 SPSFGSKSPEDVERLCFAFVLYCVAKLK-----GKGMKQESSRFRLWKILEGCKLKYNDF T.aestivum_isoX4 SPSFGSKSPEDVERLCFAFVLYCVAKLK-----GKGMKEESSRVRLWKILEGCKLKYNDF O.vulgare_isoX2 MSSLGSGSPEEIERSWSACVLYCVSKLG-----NAGKAKEDRAITLRQILRAFELKIVDF T.aestivum_isoX1 MSSLGSGSPEEIERSWCACVLYCVSKLG-----NAGKAKEDRGITLRQILRAFDLKIVDFT.aestivum_isoX5 MSSLGSGSPEEIERSWCACVLYCVSKLG-----NAGKAKEDRGITLRQILRAFDLKIVDFT.aestivum_isoX7 MSSLGSGSPEEIERSWCACVLYCVSKLG-----NAGKAKEDRGITLRQILRAFDLKIVDF T.aestivum_isoX6 MSSLGSGSPEEIERSWCACVLYCVSKLG-----NAGKAKEDRGITLRQILRAFDLKIVDF O.sativa_isoX1 MSSLGSGSPEEIERFWSAFVLYCVSRLG-----KAGKGKEDGGISLCQILRAFSLNIVDF Z.mays_isoX6 MSSLGGGSPEEVERFWSACVMYCVSRLS-----KARRSKESGSVSLCQILRASKLNIVDFS.bicolor_isoX2 VSSLGGGSPEEIERFWSACVLYCVSRLS-----KAGRSKEDGSVSLCQILRASKLNIVDFZ.mays_isoX4 MSSLGGGSPEEIERFWSACVLYCVSRLS-----KAGRSKEDGSVSLCQILRASKLNIVDF A.thaliana MSNIGSGTREEVERFWFAFILYSVKRLSVRKEADGLSVSGDNEFNLCQILRALKLNIVDF B.napus_isoX4 MANIGSGTREEVERFWFAFILYSVKMLTVRKQVDGQSVSGDNGFNLCQILRALKLNIVDF B.rapa_isoX3 MANIGSGTREEVERFWFAFILYSVKMLTVRKQVDGQSVSGDNGFNLCQILRALKLNIVDF B.rapa_isoX2 MANIGSGTREEVERFWFAFILYSVKMLTVRKQVDGQSVSGDNGFNLCQILRALKLNIVDF B.rapa_isoX4 MANIGSGTREEVERFWFAFILYSVKMLTVRKQVDGQSVSGDNGFNLCQILRALKLNIVDF B.napus_isoX3 MANIGSGTREEVERFWFAFVLYSVKMLTVRKQVDGQSVSGDNKFNLCQILRALKLNIVDF B.oleracea_isoX2 MANIGSGTREEVERFWFAFVLYSVKMLTVRKQVDGQSVPVDNRFNLCQILRALKLNIVDF B.oleracea_isoX1 MANIGSGTREEVERFWFAFVLYSVKMLTVRKQVDGQSVPVDNRFNLCQILRALKLNIVDF B.rapa_isoX1 MANIGSGTREEVERFWFAFVLYSVKMLTVRKQVDGQSVSGDNKFNLCQILRALKINIVDF B.napus_isoX2 MANIGSGTREEVERFWFAFVLYSVKMLTVRKQVDGQSVSGDNKFNLCQILRALKLNIVDF B.napus_isoX1 MANIGSGTREEVERFWFAFVLYSVKMLTVRKQVDGQSVSGDNKFNLCQILRALKLNIVDF S.tuberosum NAAIGAITPEEVERYWFAFVLFSVKRLSENEARNPSNGNEGKGFDLCQILRGAKLNVLDF S.pennellii NAAIGAITPEEVERYWFVFVLFSVKRLSENEARNSSNGNEGNGFDLCQILRGAKLNVLDF S.lycopersicum NAAIGAITPEEVERYWFVFVLFSVKRLSENEARNSSNGNEGNGFDLCQILRGAKLNVLDF N.bentamiana NSTIGVITQEGVERYWFVFVLYSVKRLSEKEAGNSNNGDKGNAFSLCQILRGAKLNVVDF N.tabacum NSTIGVITQEGVERYWFVFVLYSVKRLSEKEVGNSSNGNEGNAFSLCQILRGAKLNVVDF V.vinifera LSAIGNG-PEDSERYWFAFILYSVKRLSEGTAENVQQGNDENGFNLCQILRVSKLNIVDF G.max_isoX3 FSSMGNGTSEEAERYWFAFILYSVKKLIQKNEEGGKEDAENTGLSLCRILRAAKLNIADF G.max_isoX1 FSSMGNGTSEEAERYWFAFILYSVKKLIQKNDEGEKEDTENIGLSLCRILRAAKLNIADF G.max_isoX2 FSSMGNGTSEEAERYWFAFILYSVKKLIQKNDEGEKEDTENTGLSLCRILRATKLNIADF S.bicolor_isoX1 --------------------------QQPKQLEGVTVLLKEASRF-YRRAYNELFSGGTT S.bicolor_isoX3 --------------------PSPATSTQQKQSESLVNLLAEASRF-YRRAYNELFSGVTT Z.mays_isoX1 --------------------------QQQKQLESLVNLLTQGSRF-YRKAYNELFSGVTT Z.mays_isoX3 ---------------------------QQKQLESLVNLLTQGSRF-YRKAYNELFSGVTT Z.mays_isoX2 ---------------------------QKKQSESLVNLLAEASRF-YRKAYNELFSGLIT Z.mays_isoX5 -------------------------RIQQKQSESLVNLLAEASRF-YRKAYNELFSGLIT O.sativa_isoX2 FKESQRLASRIDQVLGSRYGSDWEARLELKQLENLVNLLADASRF-YCKAFNELFLSPST O.vulgare_isoX1 FKGSQQLLSKLDHVLRSRYGTDWEDLLELKQLQSLVNLLADASRF-YCKAFNELFLSAST T.aestivum_isoX3 FKESQQLLSKIDHVLRSWYGTDWEDQLELKQLQSLVNLLADASRF-YCKAFNELFLNAST T.aestivum_isoX2 FKESQQLLSKIDHVLRGRYGTDWEDQLELKQLQSLVNLLADASRF-YCKAFNELFLSAST T.aestivum_isoX4 FKESQQLLSKIDHVLRSRYGTDWEDQLELKQLQSLVNLLADASRF-YCKAFNELFLSAST O.vulgare_isoX2 FKEMPQFCIKVGFILTGLYGSDWEKRLELQELQANLVHLCSLGRQHYRRAYQELFLLNDG T.aestivum_isoX1 FKEMPQFCIKVGFILTGLYGSDWEKRLELQELQANLVHLCSLGRH-YRRAYQELFLLNDG T.aestivum_isoX5 FKEMPQFCIKVGFILTGLYGSDWEKRLELQELQANLVHLCSLGRH-YRRAYQELFLLNDG T.aestivum_isoX7 FKEMPQFCIKVGFILTGLYGSDWEKRLELQELQANLVHLCSLGRH-YRRAYQELFLLNDG T.aestivum_isoX6 FKEMPQFCIKVGFILTGLYGSDWEKRLELQELQANLVHLCSLGRH-YRRAYQELFLLNDG O.sativa_isoX1 FKEMPQFCIKVGSVLAGLYGSDWEKRLELKELQANVVHLSLLSRY-YKRAYQELFLLNDA Z.mays_isoX6 FKEMPQFCIKVAHILTGLYGSDWEKRLELKELQANVVHLSLLSRH-YKRAYQELFLPNDG S.bicolor_isoX2 FKEMPQFCLKVAHVLTGLYGSDWEKRLELKELQANVVHLSLLSRH-YKRAYQELFLSNDG Z.mays_isoX4 FKEMPQFCIKVAHILTGLYGSDWEKRLELKELQANVVHLSLLSSY-YKRAYQELFLSNDG A.thaliana FKELPQFVVKAGSVLGELYGADWENRLQAKEVQANFVHLSLLSKY-YKRGFREFFLTYDA B.napus_isoX4 FKELPQFVVKAGPVLCELYGADWENRLQAKELQANFVHLSLLSKY-YKRGYREFFLTYDA B.rapa_isoX3 FKELPQFVVKAGPVLCELYGADWENRLQAKELQANFVHLSLLSKY-YKRGYREFFLTYDA B.rapa_isoX2 FKELPQFVVKAGPVLCELYGADWENRLQAKELQANFVHLSLLSKY-YKRGYREFFLTYDA B.rapa_isoX4 FKELPQFVVKAGPVLCELYGADWENRLQAKELQANFVHLSLLSKY-YKRGYREFFLTYDA B.napus_isoX3 FKELPQFAVKAGPVLCELYGADWENRLQAKELQANFVHLSLLSKY-YKRGYQEFFLTYDA B.oleracea_isoX2 FKELPQFVVKAGPVLCELYGADWENRLQAKELQANFVHLSLLSNY-YKRGYQEFFLTYDA B.oleracea_isoX1 FKELPQFVVKAGPVLCELYGADWENRLQAKELQANFVHLSLLSNY-YKRGYQEFFLTYDA B.rapa_isoX1 FKELPQFAVKAGPVLCELYGADWENRLQAKELQANFVHLSLLSKY-YKRGYQEFFLTYDA B.napus_isoX2 FKELPQFAVKAGPVLCELYGADWENRLQAKELQANFVHLSLLSKY-YKRGYQEFFLTYDA B.napus_isoX1 FKELPQFAVKAGPVLCELYGADWENRLQAKELQANFVHLSLLSKY-YKRGYQEFFLTYDA S.tuberosum FKELPQFIVKVGPVLSNLYGSDWEKRLQAKELQTNFVHLSLLSKY-YKRAYQELFLSSDN S.pennellii FKELPQFIVKVGPVLSNLYGSDWEKRLQAKELQTNFVHLSLLSKY-YKRAYQELFLSSDN S.lycopersicum FKELPQFIVKVGPVLSNLYGSDWEKRLQAKELQTNFVHLSLLSKY-YKRAYQELFLSSDN N.bentamiana FKELPQFILKVGPTLSNLYGSDWEKRLEVKELQTNFVHLSLLSKY-YKRAYQELFLASGN N.tabacum FKELPQFILKVGPTLSNLYGSDWEKRLEAKELQTNFVHLSLLSKY-YKRAYQELFLASGN V.vinifera FKELPQFIVKVGPILGNLYGPDWEKRLEAKELQANFVHLSILSKY-YKRAYLEFFSTSGS G.max_isoX3 FKELPQFVVKAGPTLSNLYGTDWENRLEAKEMHANAIHLKILSKY-YKRVFGEFFVSTDT G.max_isoX1 FKELPQFVVKAGPTLSNLYGTDWENRLEAKEMHANAIHLKILSKY-YKRVFGEFFVATDT G.max_isoX2 FKELPQFVVKAGPTLSNLYGTDWENRLEAKEMHANAIHLKILSKY-YKRVFGEFFVATDT ::: * . * . : *:*S.bicolor_isoX1 EQEPESSTST-----PEYMLFGWHLFLMLRSRSPELFKDLVSCIHGLVAVLAILLIHVPA S.bicolor_isoX3 EQEPESSTNT-----PEYMLFGWYLFLMLHSRSPELFKDLVSCIHGLVAVLAILLIHVPV Z.mays_isoX1 EQDPDSSTNI-----PEYMLFGWHLFLMLHLRSPELFKDLVSCIHGLVAVLAILLIHVPAZ.mays_isoX3 EQDPDSSTNI-----PEYMLFGWHLFLMLHLRSPELFKDLVSCIHGLVAVLAILLIHVPAZ.mays_isoX2 EWEPESSTNI-----PDYMLFGWHLFLNLRLRSPELFKDLVSCIHGLVAVLAILLVHVPA Z.mays_isoX5 EWEPESSTNT-----PDYMLFGWHLFLTLRSRSPELFKDLVSCIHGLVAVLAILLVHVPA O.sativa_isoX2 DQEPGSTTNI-----PDYIRFGWLLFLILRSKSPELFKDLVSCIHGLVAILAILLIHVPA O.vulgare_isoX1 GQEPGSTTSN-----PDFFCFGWLFFLALRSKSPELFKDLVSCIHGLVAILAILLIHVPA T.aestivum_isoX3 GQEPGSTKSN-----PEYFCFGWLFFLALRSKSPELFKDLVSCXXXXXXXXAILLIHVPA T.aestivum_isoX2 GQEPGSTKSN-----PEYFCFGWLIFLALRSKSPELFKDLVSCIHGLVAILAILLIHVPA T.aestivum_isoX4 GQEPGSTKSN-----PEYFCFGWLFFLALRSKSPELYKDLVSCIHGLVAILAILLIHVPA O.vulgare_isoX2 KPANNSSDLN-LQQASEYYNFGWLLFLVLRNQASSAVKNLLTSTTELVSVLAVLIIHIPV T.aestivum_isoX1 KPANNSSELN-VQQASEYYDFGWLLFLVLRNQASSAVKNLLTSTTELVSVLAVLIIHIPV T.aestivum_isoX5 KPANNSSELN-VQQASEYYDFGWLLFLVLRNQASSAVKNLLTSTTELVSVLAVLIIHIPV T.aestivum_isoX7 KPANNSSELN-VQQASEYYDFGWLLFLVLRNQASSAVKNLLTSTTELVSVLAVLIIHIPV T.aestivum_isoX6 KPANNSSELN-VQQASEYYDFGWLLFLVLRNQASSAVKNLLTSTTELVSVLAVLIIHIPV O.sativa_isoX1 KPPENSAEPN--AQASDYYRFGWLLFLVLRIQTFSRFKDLVTSTNGLVSVLAVLIVHIPV Z.mays_isoX6 KCSDSSSESN-NQEVSDYYRFGWLLFLVLRIQTFSRFKDLVTSTNELVSVLAVLIIHIPV S.bicolor_isoX2 KCSGNSSELI-NQEASDYYRFGWLLFLALRIQTFSRFKDLVTSTNELVSVLAVLIIHVPV Z.mays_isoX4 KSSDNSSESN-NQEASDYYRFGWLLFLVLRIQTFSRFKDLVTSTNELVSVLAVLIIHVPV A.thaliana NAEKNSANSS--TYLLDSYRFGWLLFLALRNHAFSRFKDLVTCSNGVVSILAILIIHVPC B.napus_isoX4 NAEKTSANSA--SYLPDSYRFGWLLFLALRNHAFSRFKDLVTCTNGLVSVLAILIIHVPC B.rapa_isoX3 NAEKTSANSA--SYLPDSYRFGWLLFLALRNHAFSRFKDLVTCTNGLVSVLAILIVHVPC B.rapa_isoX2 NAEKTSANSA--SYLPDSYRFGWLLFLALRNHAFSRFKDLVTCTNGLVSVLAILIVHVPC B.rapa_isoX4 NAEKTSANSA--SYLPDSYRFGWLLFLALRNHAFSRFKDLVTCTNGLVSVLAILIVHVPC B.napus_isoX3 NAEKTSANSA--SYLPDSYRFGWLLFLALRNHAFSRFKDLVTCTNGLVSILAILIIHVPC B.oleracea_isoX2 NAERASANSA--SYLPDSYRFGWLLFLALRNHAFSRFKDLVTCTNGLVSILAILIIHVPC B.oleracea_isoX1 NAERASANSA--SYLPDSYRFGWLLFLALRNHAFSRFKDLVTCTNGLVSILAILIIHVPC B.rapa_isoX1 NAEKTSANSA--SYLPDSYRFGWLLFLALRNHAFSRFKDLVTCTNGLVSILAILIIHVPC B.napus_isoX2 NAEKTSANSA--SYLPDSYRFGWLLFLALRNHAFSRFKDLVTCTNGLVSILAILIIHVPC B.napus_isoX1 NAEKTSANSA--SYLPDSYRFGWLLFLALRNHAFSRFKDLVTCTNGLVSILAILIIHVPC S.tuberosum NEDKQSATSNSIILLPQYYRFGWLLFLSLREHVFSRFKDLVTCTNGLVSVLAMLIIHVPV S.pennellii NEVNQSATSNSIVLLPQYYRFGWLLFLSLREHAFSRFKDLVTCTNGLVSVLAILIIHVPV S.lycopersicum NEVNQSATSNSIVLLPQYYRFGWLLFLSLREHVFSRFKDLVTCTNGLVSVLAILIIHVPV N.bentamiana NEDKPSATSNSAIHLPQYYRFGWLLFLSLRIHVFSRFKDLVTCTNGLVSVLAILMIHVPV N.tabacum NEDKPSATSSSAIHLPQYYRFGWLLFLSLRIHVFSRFKDLVTCTNGLVSVLAILIIHVPV V.vinifera NLDKQSSVISASGYVSDYHRFGWLLFLALRVHAFSRFKDLVTCTNGLVSILAILILHVPI G.max_isoX3 NAEKNSSITV---HASEYHRFGWLLFLALRVHAFSRFKDLVTCTNGLISILAILIIHVPT G.max_isoX1 NAEINSPITV---HASEYHRFGWLLFLALRVHAFSRFKDLVTCTNGLISILAILIIHVPT G.max_isoX2 NAEINSPITV---HASEYHRFGWLLFLALRVHAFSRFKDLVTCTNGLISILAILIIHVPT *. : *** :** *. . . *:*::. *:*::*:* S.bicolor_isoX1 KFRRFMIDGSSHLIKQNEKGMDLIASLCHNYHASEECVKEMMDKSHKAIEDIFGTKALSA S.bicolor_isoX3 KFRSFTVEGSSHLIKQTEKGMDLIASLCHNYHASEERLKEMMDKSHKAIEDVFGMKALSA Z.mays_isoX1 KFRSFTIEGSSHLIKQTEKGVDLIASLCHNYHTSEERLKEMLHKSHNAIEDIFHMKALSA Z.mays_isoX3 KFRSFTIEGSSHLIKQTEKGVDLIASLCHNYHTSEERLKEMLHKSHNAIEDIFHMKALSA Z.mays_isoX2 KFRTFTIEGSSHLIKQTEKGVDLIASLCHNYHTSEECLKEMMDKSHKAIEEVFSMKALSA Z.mays_isoX5 KFRTFTIEGSSHLIKQTEKGVDLIASLCHNYHTSEECLKEMMDKSHKAIEEVFSMKALSA O.sativa_isoX2 KFRSFTIEGSSHLIKQTEKGVDLLPSLCHNYHTSEDRLKEMMGKSYKVIEVFFSRKAINA O.vulgare_isoX1 KFRSFTIEGSSHLIKQTVRGVDLLASLCHNYHTSENRLKEMMGKFHKAIEEFFSTKAVRA T.aestivum_isoX3 KFRSFTIEGSSHSIKQTVRGVDLLASLCHNYHTSEDRLKEMMGKFHKAIEVFFSTKAVRA T.aestivum_isoX2 KFRSFTIEGSSHLIKQTVRGVDLLASLCHNYHTSEDRLREMMGKFHKAIEEFFSTKAVRA T.aestivum_isoX4 KFRSFTIEGSSHLIKQTVRGVDLLASLCHNYHTSEDRLKEMMGKFHKAIEVFFSTKAVRA O.vulgare_isoX2 RLRNFSIEDSSCFAKKSEKGVNLIASLCERYLTSEDELSKALQKTNILITDILKKKPCSD T.aestivum_isoX1 RLRNFSIEDSSCFAKKSDKGVNLIASLCERYLTSEDELSKALQKTNILIKDILKKKPCSD T.aestivum_isoX5 RLRNFSIEDSSCFAKKSDKGVNLIASLCERYLTSEDELSKALQKTNILIKDILKKKPCSD T.aestivum_isoX7 RLRNFSIEDSSCFAKKSDKGVNLIASLCERYLTSEDELSKALQKTNILIKGILKKKPCSD T.aestivum_isoX6 RLRNFSIEDSSCFAKKSDKGVNLIASLCERYLTSEDELSKALQKTNILIKDILKKKPCSD O.sativa_isoX1 RLRNFNIKESSSFAKKSDKGVNLIASLCEKYHTSEDELSKAIEKTNTLIVDILKKKPCPA Z.mays_isoX6 RLRNFDIKDSSYFGKKSDRGVSLIASLCEKYHTSEDELSKALEKTNTLILDILKKKPWTA S.bicolor_isoX2 RLRNFDIKDSSYFAKKSDRGVSLIASLCEKYHTSEDELSKALEKTNTLIMDVLKKKPCPA Z.mays_isoX4 RLRNFDIKDSSYFGKKSDRGVSLIASLCEKHHTSEDELSKALEKTNTLIMDILKKKPCPA A.thaliana RFRNFSIQDSSRFVKKGDKGVDLVASLCKIYDASEDELRIVIDKANNLVETILKKKPSPA B.napus_isoX4 RFRNFSIQDSSRFVKKGDKDVDLVASLCKIYDASEDELRKIMNKANSLIETILKKRPSPA B.rapa_isoX3 RFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMNKANSLIETILKKKPSAA B.rapa_isoX2 RFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMNKANSLIETILKKKPSAA B.rapa_isoX4 RFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMNKANSLIETILKKKPSAAB.napus_isoX3 RFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMDQANNLIETILKKKPSSAB.oleracea_isoX2 RFRNFNIQDSSRFVKKGDKEVDLVASLCKIYDASEDELKKIMDQANNLIETILKKKPSSA B.oleracea_isoX1 RFRNFNIQDSSRFVKKGDKEVDLVASLCKIYDASEDELKKIMDQANNLIETILKKKPSSA B.rapa_isoX1 RFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMDQANNLIETILKKKPSSA B.napus_isoX2 RFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMDQANNLIETILKKKPSSAB.napus_isoX1 RFRNFSIQDSSRFVKKGDKEVDLVASLCKIYDASEDELRKIMDQANNLIETILKKKPSSAS.tuberosum RYRNFNINDSPRFVKKGDK-VDLLVSLSSIYQTSIDDLRETMDKVNNLITVKLKKEPCLA S.pennellii CFRNFNINDSPRFVKKGDK-VDLLVSLSSIYQTSIDDLRETMDKVNNLITVKLKKELCLA S.lycopersicum CFRNFNINDSPRFVKKGDK-VDLLVSLSSIYQTSIDDLRETMDKVNNLITVKLKKELCLA N.bentamiana RFRNFNIDDSSRFVKKGDK-VDLLASLSKIYQTSIDDLRETMDKVNNLITEKLKKKPCLA N.tabacum RFRNFNINDSSRFVKKGDK-VDLLASLGKIYQTSIDDLRETMDKVNYLITEKLKKKPCLA V.vinifera SFRSFTINNYPRFVKKGNKGMDLLASLCDIYETSEDEVRKTMEQTNKVIVDILKKKPCLA G.max_isoX3 RFRNFNIHDSSRFVKKSNKGVDLLASLCNIYNTSEDELRKTMEKANNVIADILKKQPCLA G.max_isoX1 RFWNFNIHDSSRFVKKSNKGVDLLASLCNIYNTSEDELRKTMEKANNVIADILKKQPCLA G.max_isoX2 RFRNFNIHDSSRFVKKSNKGVDLLASLCNIYNTSEDELRKTMEKANNVIADILKKQPCLA . * : . *: . :.*: ** : :* : : : : : : S.bicolor_isoX1 -SEGKTENLDKIDTD-------GLMYFKGLIDTECFQSNLEKMEKLCNPNSCE-GELDYK S.bicolor_isoX3 -SECKTENLDMIGTD-------GLMYFKGLIDMECFQSNLEKMEKLCNSNSSE-GKLDFK Z.mays_isoX1 -SECKPENLDKIDTD-------DLMYFKGLIDMECFQSNLEKMEKLCNSNSCK-GELDFK Z.mays_isoX3 -SECKPENLDKIDTGMEPFLTYDLMYFKGLIDMECFQSNLEKMEKLCNSNSCK-GELDFK Z.mays_isoX2 -SECKTENLDKIDTD-------RLMYFKGLIDMECFQSNLEKIEKLCNSNNCE-AELDFK Z.mays_isoX5 -SECKTENLDKIDTGMEPFLAYRLMYFKGLIDMECFQSNLEKMEKLCNSNNCE-AELDFK O.sativa_isoX2 -SEFKTVNLDKIDTD-------GLMYFKDLVDDEIFQSNLEKLEKLSSTTGCQ-GELDLE O.vulgare_isoX1 -SECKTETSGKIDTD-------GLIYFRDLLDKECFHSNFEKLEKLTTTTSWE-GELDLE T.aestivum_isoX3 -SECKIETLDKIDTD-------GLIYFRDLLDKECFHSNFEKLEKLSSTTSWE-GELDLK T.aestivum_isoX2 -SECKTETLDKIDTD-------GLIYFRDLLDKECFHSNFEKLEKLSSTTSWE-GELDLK T.aestivum_isoX4 -SECKLETLDKIDTD-------GLIYFRDLLDKECFHSNFEKLEKLSSTTSWE-GELDLK O.vulgare_isoX2 VSECQQGCLSFIDPE-------GLVFFKNFLEEDSLKSSLQVLEREYENGLDTKGELDAR T.aestivum_isoX1 VSECQQGSLSFIDPE-------GLTFFKNFLEEDSLKSSLQVLEKEYVNGLDTKGELDAR T.aestivum_isoX5 VSECQQGSLSFIDPE-------GLTFFKNFLEEDSLKSSLQVLEKEYVNGLDTKGELDAR T.aestivum_isoX7 VSECQQGSLSFIDPE-------GLTFFKNFLEEDSLKSSLQVLEKEYVNGLDTKGELDAR T.aestivum_isoX6 VSECQQGSLSFIDPE-------GLTFFKNFLEEDSLKSSLQVLEKEYVNGLDTKGELDAR O.sativa_isoX1 ASECQQDRLSFIDPE-------GLTYFKNLLEEDSLKLSLLMLEKEYENAINTKGELDER Z.mays_isoX6 TSACQQDNLTFIDPE-------GLTVFKDLLQGGSLKSSLLILEKEYENAINTRGELDER S.bicolor_isoX2 TSACQQDNLSFIDPE-------GLTVFKDFLQGDSLKSSLLILEKEYENAINTKGELDER Z.mays_isoX4 TSACQQDNLSFIDPE-------GLTVFKDLLQGDSLKPSLIILEKEYENAINTKGELDER A.thaliana -SECQTDKLDNIDPD-------GLTYFEDLLEETSISTSLITLEKDYYDGK---GELDER B.napus_isoX4 -STCKTDTLDNIITD-------GLTYFEDLLDESSISTSLTTLEKDYDETVCNKGELDER B.rapa_isoX3 -SACKTDTLDNINTD-------GLTYFEDLLDESSISTSLTTLEKDYDETVCNKGELDER B.rapa_isoX2 -SACKTDTLDNINTD-------GLTYFEDLLDESSISTSLTTLEKDYDETVCNKGELDER B.rapa_isoX4 -SACKTDTLDNINTD-------GLTYFEDLLDESSISTSLTTLEKDYDETVCNKGELDER B.napus_isoX3 -STCKIDKLDNINPD-------GLTYFEDLLDETSISTSLITLEKDYEDSVCNKGELDER B.oleracea_isoX2 -STCKIDKLDNINPD-------GLTYFEDLLDEASISTSSITLEKDYEDSVCNKGELDER B.oleracea_isoX1 -STCKIDKLDNINPD-------GLTYFEDLLDEASISTSSITLEKDYEDSVCNKGELDER B.rapa_isoX1 -STCKIDKLDNINPD-------GLTYFEDLLDETSISTSLITLEKDYEDSVCNKGELDER B.napus_isoX2 -STCKIDKLDNINPD-------GLTYFEDLLDETSISTSLITLEKDYEDSVCNKGELDER B.napus_isoX1 -STCKIDKLDNINPD-------GLTYFEDLLDETSISTSLITLEKDYEDSVCNKGELDER S.tuberosum -SECRAENLDNIDAD-------GLTYFENLLEESSLSSSICILEKDYNDAIQNKGELDER S.pennellii -SECRAKNLDNIDAD-------GLTYFENLLEESSLSSSICILEKDYNDAIQNKGELDER S.lycopersicum -SECRAKNLDNIDAD-------GLTYFENLLEESSLSSSICILEKDYNDAIQNKGELDER N.bentamiana -SEFRTENLDNLDTD-------GLTYFEDLMEESCLSSSVSTLEKDYSDAIQNKGELDER N.tabacum -SEFRTENLDNLDTD-------GLTYFEDLMEESCLPSSVSILEKDYSDAIQNKGELDER V.vinifera -SECKSENLASIDPD-------GLVYFEDLMDESSLSSSINILEKDYDAAIRNKGELDER G.max_isoX3 -SECETENLENIDKD-------GLTYFKDLMEESSLPSSLNMLEKDYDYMIRNKSELDER G.max_isoX1 -SECETGNLENIDKD-------GLTYFKDLMEESSLPSSLSMLEKDYDYMIHNKSELDER G.max_isoX2 -SECETENLENIDKD-------GLTYFKDLMEESSLPSSLSMLEKDYDYMIRNKSELDER * : * * .::: : . :*. .:** S.bicolor_isoX1 SILINNDYIPCAENSPGDSTNLGHSKRVF-EILASPTKTIKNMLTVPSSP-LSPATGGS- S.bicolor_isoX3 SILINNDYIPCAENLSGDSTNLGHSKHVF-EILASPTKTIKNMLTVPSSP-LSPATSGS- Z.mays_isoX1 SILINNDYIPYDENSTGDSTNLGHSKCAF-ETLASPTKTIKNMLTVPSSP-LSPATGGS- Z.mays_isoX3 SILINNDYIPYDENSTGDSTNLGHSKCAF-ETLASPTKTIKNMLTVPSSP-LSPATGGS- Z.mays_isoX2 LILTNNDYIPCAENLSRDSTNLGCSKCAF-ETLASPRKTIKNMLTVPSSP-LSPTNGCS- Z.mays_isoX5 LILTNNDYIPCAENLSRDSTNLGCSKCAF-ETLASPTKTIKNMLTVPSSP-LSPTNGGS- O.sativa_isoX2 MFLTSNDYVLNAENSSGSSANFGCSKRVF-ETLASPTKTIKNMLAAPSSP-SSPANGGS- O.vulgare_isoX1 MFLVNNGNIISAENSSRDFSNLSCPKRVF-ETLASPAKTIKNMLTVPSSP-SSPANGGS- T.aestivum_isoX3 RFLINNDNIISAENSSRDFTNLSCPKRVF-ETLASPTKTIKNMLTVPSSP-SSPANGGS- T.aestivum_isoX2 RFLINNDNIISAENSSRDFTNLSCPKRVF-ETLASPTKTIKNMLTVPSSP-SSPANGGS-T.aestivum_isoX4 RFLINNDNIISAENSSRDFTNLSCPKRVF-ETLASPTKTIKNMLTVPSSP-SSPVNGGS-O.vulgare_isoX2 MFANDEDSLLGSGSLSGGALKLPGTKRKYDDVMASPTKSTASRA-PMSPPRFCPSPNGNS T.aestivum_isoX1 MFANEEDSLLGSGSLSGGALKLPGTKRKYDDVMASPTKSTASRA-PMSPPRFCPSPNGNS T.aestivum_isoX5 MFANDEDSLLGSGSLSGGALKLPGTKRKYDDVMASPTKSTASRA-PMSPPRFCPSPNGNS T.aestivum_isoX7 MFANEEDSLLGSGSLSGGALKLPGTKRKYDDVMASPTKSTASRA-PMSPPRFCPSPNGNST.aestivum_isoX6 MFANDEDSLLGSGSLSGGALKLPGTKRKYDDVMASPTKSTASRA-PMSPPRFCPSPNGNSO.sativa_isoX1 MFANDEDSLLGSGSLSGGAINLPGTKRKY-DVMASPAKSITSPS-PMSPPRFCASPTGNG Z.mays_isoX6 MFANDEDSLLGSGSLSGGAINLPGTKRKY-DVMASPAKSISSPN-PMSPPRFCLSPKGNS S.bicolor_isoX2 MFANDEDSLLGSGSLSGGAINLPGTKRKY-DVMASPAKSISSPN-PMSPPRFCLSPKGSN Z.mays_isoX4 MFANDEDSLLGSGSLSGGAINLPGTKRKY-DVMASPAKSISSPN-PMSPPRFCLSPKGNG A.thaliana VFINEEDSLLGSGSLSAGAVNITGVKRKI-DALSSPARTFISPLSPHKSP-AAKTNGISG B.napus_isoX4 VFINEEDSLLGSGSLSAGAVNLTGAKRKI-DSLSSPARTFISPLSPHKSP-AAKTTV--- B.rapa_isoX3 VFINEEDSLLGSGSLSAGAVNLTGAKRKI-DSLSSPARTFISPLSPHKSP-AAKTTV--- B.rapa_isoX2 VFINEEDSLLGSGSLSAGAVNLTGAKRKI-DSLSSPARTFISPLSPHKSP-AAKTTV--- B.rapa_isoX4 VFINEEDSLLGSGSLSAGAVNLTGAKRKI-DSLSSPARTFISPLSPHKSP-AAKTTV--- B.napus_isoX3 VFINEEDSLLGSGSLSAGAVNVTGVKRKI-DSLSSPARTFISPLSPHKSP-AAKTNTISG B.oleracea_isoX2 VFINEEDSLLGSGSLSAGAVNVTGVKRKI-DSLSSPARTFISPLSPHKSP-AAKTNTISG B.oleracea_isoX1 VFINEEDSLLGSGSLSAGAVNVTGVKRKI-DSLSSPARTFISPLSPHKSP-AAKTNTISG B.rapa_isoX1 VFINEEDSLLGSGSLSAGAVNVTGVKRKI-DSLSSPARTFISPLSPHKSP-AAKTNTISG B.napus_isoX2 VFINEEDSLLGSGSLSAGAVNVTGVKRKI-DSLSSPARTFISPLSPHKSP-AAKTNTISG B.napus_isoX1 VFINEEDSLLGSGSLSAGAVNVTGVKRKI-DSLSSPARTFISPLSPHKSP-AAKTNTISG S.tuberosum IFVNDEDSLLGSGSLSGGAVNMNGIKKKF-DAMASPTKTITSPLSPYRSPGASNVNNNPN S.pennellii IFVNDEDSLLGSGSLSGGAVNMNGIKKKF-DAMASPTKTITSPLSPYRSPGASNVNSNPN S.lycopersicum IFVNDEDSLLGSGSLSGGAVNMNGIKKKF-DAMASPTKTITSPLSPYRSPGASNVNSNLN N.bentamiana IFVNEEDSLLGSGSLSGGAVNMNGTKRKF-DAMASPAKTVTSTLSPYRSP---------N N.tabacum IFVNEEDSLLGSGSLSGGVVNMNGVKRKF-DAMASPAKTVTSTLSPYRSP---------N V.vinifera VFINGEDSLLGSGSLSGGAMSISGAKRKI-DSLASPAKTITSPLSPNRSP------GILG G.max_isoX3 LFINEDDSLLASVSLSGGSVSVGGVKRKF-DSMASPAKTITSPLSPHRSP-TSHTNGIPG G.max_isoX1 LFINEDDSLLASVSLSGGSVSAGGVKRKF-DSMASPAKTITSPLSPHRSP-ASHANGIPG G.max_isoX2 LFINEDDSLLASVSLSGGSVSAGGVKRKF-DSMASPAKTITSPLSPHRSP-ASHANGIPG : :. : . . . . * : ::** .: . .* S.bicolor_isoX1 ---VKILQMTPVTSAMTTAKWLREVISSLPEKPSSKLQQLLSSCDRDLTNAVTERVSIVL S.bicolor_isoX3 ---VKIVQMTPVTSAMTTAKWLREVISSLPEKPSSKLQQLLSSCDRDLTNAVTERVSIVL Z.mays_isoX1 ---VKIVQMTPVTSAMTTAKWLREVISSLPDKPSSKLQQFLSSCDRDLTNAVTERVSIVL Z.mays_isoX3 ---VKIVQMTPVTSAMTTAKWLREVISSLPDKPSSKLQQFLSSCDRDLTNAVTERVSIVL Z.mays_isoX2 ---VKIVQMTPITSAMTTAKWLREVISSLPEKPSSKLQQLMSSCDRDLTYAVTERVSIVL Z.mays_isoX5 ---VKIVQMTPITSAMTTAKWLREVISSLPEKPSSKLQQLMSSCDRDLTNAVTERVSIVL O.sativa_isoX2 ---IKIVQMTPVTSAMTTAKWLRDVISSLPDKPSSKLEEFLSSCDTDLTSDVVKRVSIIL O.vulgare_isoX1 ---VKVVQMTPVTSAMTTAKWLHEVISSLPEKPSTKLEKFLSSCDTDLTSDVTKRVSIIL T.aestivum_isoX3 ---VKVVQMTPVTSAMTTAKWLREVISLLPEKPSSKLEEFLSSCDTDLTSDVTKRVSIIL T.aestivum_isoX2 ---VKVVQMTPVTSAMTTAKWLREVISLLPEKPSSKLEKFLSSCDTDLTSDVTKRVSIIL T.aestivum_isoX4 ---VKVVQMTPVTSAMTTAKWLRGVISSLPEKPSSKLEKFLSSCDTDLTSDVTKRVSIIL O.vulgare_isoX2 FCNSKMAPFTPVSTAMTTAKWLRSTISPLPSKPSGELLRFFSACDKDVTDDITCRAAIIL T.aestivum_isoX1 FCNSKMAPFTPVSTAMTTAKWLRSTISPLPSKPSGELLRFFSACDKDVTDDITCRAAIIL T.aestivum_isoX5 FCNSKMAPFTPVSTAMTTAKWLRSTISPLPSKPSGELLRFFSACDKDVTDDITCRAAIIL T.aestivum_isoX7 FCNSKMAPFTPVSTAMTTAKWLRSAISPLPSKPSGELLRFFSACDKDVTDDITCRAAIIL T.aestivum_isoX6 FCNSKMAPFTPVSTAMTTAKWLRSTISPLPSKPSGELLRFFSACDKDVTDDIICRAAIIL O.sativa_isoX1 YCSSKMAPITPVSTAMTTAKWLRSTISPLPSKPSGELLRFFSACDKDVTDDITRRAGIIL Z.mays_isoX6 FCNSKMAPITPVSTAMTTAKWLRSTVSPLPSKPSGELLRFFSACDKDVTDDIVHRAGIIL S.bicolor_isoX2 FCNSKMAPITPVSTAMTTAKWLRSTVSPLPSKPSGELLRFFSACDKDLTDDIAHRAGIIL Z.mays_isoX4 FCNSKMAPSTPVSTAMTTAKWLRNTVSPLPSRPSGELLRFFSACDKDLTDDIAHRAGIIL A.thaliana A--TKL-AATPVSTAMTTAKWLRTVISPLLPKPSPGLEHFLKSCDRDITNDVTRRAHIIL B.napus_isoX4 -------TATPVSTAMTTAKWLRTVICPLPPKPSPGLELFLKSCDRDITNDVTRRAHVIL B.rapa_isoX3 -------TATPVSTAMTTAKWLRTVICPLPPKPSPGLELFLKSCDRDITNDVTRRAHVIL B.rapa_isoX2 -------TATPVSTAMTTAKWLRTVICPLPPKPSPGLELFLKSCDRDITNDVTRRAHVIL B.rapa_isoX4 -------TATPVSTAMTTAKWLRTVICPLPPKPSPGLELFLKSCDRDITNDVTRRAHVIL B.napus_isoX3 A--IKL-TATPVSTAMTTAKWLRTVICPLLPKPSPGLEHFLKSCDRDITTDVTRRAHVIL B.oleracea_isoX2 A--IKL-TATPVSTAMTTAKWLRTVICPLLPKPSPGLEHFLKSCDRDITTDVTRRAHVIL B.oleracea_isoX1 A--IKL-TATPVSTAMTTAKWLRTVICPLLPKPSPGLEHFLKSCDRDITTDVTRRAHVIL B.rapa_isoX1 A--IKL-TATPVSTAMTTAKWLRTVICPLLPKPSPGLEHFLKSCDRDITTDVTRRAHVIL B.napus_isoX2 A--IKL-TATPVSTAMTTAKWLRTVICPLLPKPSPGLEHFLKSCDRDITTDVTRRAHVIL B.napus_isoX1 A--IKL-TATPVSTAMTTAKWLRTVICPLLPKPSPGLEHFLKSCDRDITTDVTRRAHVIL S.tuberosum CGNSKM-AATPVTTAMTTARWLRTVIAPLQPKPSPELERFLSACDRNVSADVIRRAHIIL S.pennellii CGNSKM-AATPVTTAMTTARWLRTVIAPLQAKPSPELERFLSACDRNVSADVIRRAHIIL S.lycopersicum CGNSKM-AATPVTTAMTTARWLRTVIAPLQAKPSPELERFLSACDRNVSADVIRRAHIIL N.bentamiana CANSKMTAATPVSTAMTTARWLRTVIAPLQPKPSAELERFLSSCDRDVTADVIRRAQIIL N.tabacum CANSKMTVATPVSTAMTTARWLRTVIAPLQPKPSAELERFLSSCDRDVTADVIRRAQIIL V.vinifera GANSKM-APTPVTTAMTTAKWLRTVISPLPSKPSAELERFLTSCDKDVTSDVIRRANVILG.max_isoX3 SANSKM-AATPVSTAMTTAKWLRTVISPLPSKPSPELERFLTSCDRDVTSDVVRRAQIILG.max_isoX1 SANSKM-AATPVSTAMTTAKWLRTVISPLPSKPSPELERFLTSCDRDATSDVVRRAQIIL G.max_isoX2 SANSKM-AATPVSTAMTTAKWLRTVISPLPSKPSPELERFLTSCDRDATSDVVRRAQIIL **:::*****.**. .:. * .** * ::.:** : : : *. ::*S.bicolor_isoX1 EAIFPTKSSADRG-GSLGISCANAFDIPWAEARKMEASKLYYRVLEAICRAELQNNNVNNS.bicolor_isoX3 EAIFPTKSSADGG-GSLGLNCANVFDIPWAEARKMEASKLYYRVLEAICRAELQNSNVNN Z.mays_isoX1 EAIFPTKSSANRG-VSLGLNCANAFDIPWAEARKVEASKLYYRVLEAICRAELQNSNVNN Z.mays_isoX3 EAIFPTKSSANRG-VSLGLNCANAFDIPWAEARKVEASKLYYRVLEAICRAELQNSNVNN Z.mays_isoX2 EAIFPTKSSADRG-GSLGLNCANAFDTLWADARKMEASKLYYRVLEAICRAELQNSNVNN Z.mays_isoX5 EAIFPTKSSADRG-GSLGLNCANAFDTLWADARKMEASKLYYRVLEAICRAELQNSNVNN O.sativa_isoX2 EAIFPTKSI-DRG-TSIGLNCANAFDIPWAEARKMEASKLYYRVLEAICRAESQNNNVNN O.vulgare_isoX1 EAIFPTKPSGHCG-GSMGLNCTNAFDIPWAEARKMEASKLYYRVLEAVCRAESLNTNVNN T.aestivum_isoX3 EAIFPTKPSGHWA-GSMGLNCTNAFDIPWAEARKMEASKLYYRVLEAICRAESLNTNVNN T.aestivum_isoX2 EVIFPTKPSGHWS-GSMGLNCTNAFDIPWAQARKMEASKLYYRVLEAICRAESLNTNVNN T.aestivum_isoX4 EAIFPTKPSGHWG-GSMGLNCTNAFDIPWAEARKMEASKLYYRVLEAICRAESLNTNVNN O.vulgare_isoX2 GAIFTGSSFGERM-CT-SLRNTNGMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGSN T.aestivum_isoX1 GAIFTGSSFGERM-CT-SLRNTSGMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGSN T.aestivum_isoX5 GAIFTGSSFGERM-CT-SLRNTSGMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGSN T.aestivum_isoX7 GAIFTGSSFGERM-CT-SLRNTSGMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGSN T.aestivum_isoX6 GAIFTGSSFGERM-CT-SLRNTSGMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGSN O.sativa_isoX1 GAIFTSSSFGERI-CT-SVRSTNRIDAIWTEQRKMEALKLYYRVLESMCRAETQILSGNN Z.mays_isoX6 GAIFTSSSFGEQI-CT-NMRSANRMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGNN S.bicolor_isoX2 GAIFTSSSFGERI-CT-NMRSANRMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGNN Z.mays_isoX4 GAIFTSSSFGERI-CT-SMRSASRMDAIWTEQRKMEALKLYYRVLESMCRAESQILSGNN A.thaliana EAIFPNSSLGAQC-GGGSLQAVDLMDDIWAEQRRLEACKLYYRVLEAMCKAEAQILHANN B.napus_isoX4 EAIFPNSSLGDQC-AGGSLQPVNLMDDIWAQQRRLEAVKLYYRVLEAMCKAEAQILHVNN B.rapa_isoX3 EAIFPNSSLGDQC-AGGSLQPVNLMDDIWAQQRRLEAVKLYYRVLEAMCKAEAQILHVNN B.rapa_isoX2 EAIFPNSSLGDQC-AGGSLQPVNLMDDIWAQQRRLEAVKLYYRVLEAMCKAEAQILHVNN B.rapa_isoX4 EAIFPNSSLGDQC-AGGSLQPVNLMDDIWAQQRRLEAVKLYYRVLEAMCKAEAQILHVNN B.napus_isoX3 EAIFPNSSLGDRC-AGGSLQPVNLMDDIWAEQRRLEAVKLYYRVLEAMCIAEAQILHANN B.oleracea_isoX2 EAIFPNSSLGDRC-AGGSLQPVNLMDDIWAEQRRLEAVKLYYRVLEAMCIAEAQILHANN B.oleracea_isoX1 EAIFPNSSLGDRC-AGGSLQPVNLMDDIWAEQRRLEAVKLYYRVLEAMCIAEAQILHANN B.rapa_isoX1 EAIFPNSSLGDRC-AGGSLQPVNLMDDIWAEQRRLEAVKLYYRVLEAMCIAEAQILHANN B.napus_isoX2 EAIFPNSSLGDRC-AGGSLQPVNLMDDIWAEQRRLEAVKLYYRVLEAMCIAEAQILHANN B.napus_isoX1 EAIFPNSSLGDRC-AGGSLQPVNLMDDIWAEQRRLEAVKLYYRVLEAMCIAEAQILHANN S.tuberosum EAIFPSSGPGEHC-AAGSLQSTSLMDNIWAEQRRSESLKLYYRVLQTMCVAESQILHATN S.pennellii EAIFPSSGPGEHC-AAGSLQSTSLMDNIWAEQRRSESLKLYYRVLQTMCVAESQILHATN S.lycopersicum EAIFPSSGPGEHC-AAGSLQSTSLMDNIWAEQRRSESLKLYYRVLQTMCVAESQILHVTN N.bentamiana EAIFPSSGPAEHCAAGGSLQSTSLMDNIWAEQRRSEALKLYYRVLQTMCTAESQILNGNN N.tabacum EAIFPSSGPAEHCAAGGSLQSTSLMDNIWAEQRRSEALKLYYRVLQTMCTAESQILNGNN V.vinifera EAIFPSIAFGERC-VTGS---ASLMDSIWAQQRRMEAMKLYYRVLEAMCTAEAQVLHANN G.max_isoX3 QAIFPSSPLGERC-VTGSLQSANLMDNIWAEQRRLEALKLYYRVLEAMCRAEAQVFHATN G.max_isoX1 QAIFPSSPLGERC-VTGSLQSANVVDNIWAEQRRLEALKLYYRVLEAMCRAEAQVLHATN G.max_isoX2 QAIFPSSPLGERC-VTGSLQSANLMDNIWAEQRRLEALKLYYRVLEAMCRAEAQVLHATN .**. . .. .* *:: *. *: *******:::* ** .* S.bicolor_isoX1 LTPLLSNERFHRCLIACSAELVLATHKPVFMMFPAVLDSTGLTAFDLSKIIENFVRHEET S.bicolor_isoX3 LTPLLLNERFHRCLIACSAELVLATHKPVFMMFPAVLESTGLTAFDLSKIIENFVRHEET Z.mays_isoX1 LTPLLSNERFHRCLIACSADLVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEET Z.mays_isoX3 LTPLLSNERFHRCLIACSADLVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEET Z.mays_isoX2 LTPLLSNERFHRCLIACSADVVLATHKTVIMMFPAVLESAGLTSFDLSKIIENFVRHEET Z.mays_isoX5 LTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTSFDLSKIIENFVRHEET O.sativa_isoX2 LTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEET O.vulgare_isoX1 LTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEES T.aestivum_isoX3 LTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEES T.aestivum_isoX2 LTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEES T.aestivum_isoX4 LTPLLSNERFHRCLIACSAELVLATHKTVIMMFPAVLESTGLTAFDLSKIIENFVRHEES O.vulgare_isoX2 LTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEDT T.aestivum_isoX1 LTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEDS T.aestivum_isoX5 LTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEDS T.aestivum_isoX7 LTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEDS T.aestivum_isoX6 LTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEDS O.sativa_isoX1 LTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEDT Z.mays_isoX6 LTLLLSNERFHRCMIACSAELVLATHKTVTLMFPAVLEKTGITAFDLSKVIEGFVRHEDT S.bicolor_isoX2 LTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIEGFVRHEDT Z.mays_isoX4 LTSLLSNERFHRCMIACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIEGFVRHEDT A.thaliana LNSLLTNERFHRCMLACSAELVLATHKTITMLFPAVLERTGITAFDLSKVIESFIRHEDS B.napus_isoX4 LNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLSKVIESFIRHEDSB.rapa_isoX3 LNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLSKVIESFIRHEDSB.rapa_isoX2 LNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLSKVIESFIRHEDS B.rapa_isoX4 LNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLSKVIESFIRHEDS B.napus_isoX3 LNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKVIESFIRHEDS B.oleracea_isoX2 LNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKVIESFIRHEDSB.oleracea_isoX1 LNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKVIESFIRHEDSB.rapa_isoX1 LNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKVIESFIRHEDS B.napus_isoX2 LNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKVIESFIRHEDS B.napus_isoX1 LNSLLTNERFHRCMLACSAELVLATHKSITMLFPAVLERTGITAFDLCKVIESFIRHEDS S.tuberosum LTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITSFDLSKVIESFIRHEES S.pennellii LTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITSFDLSKVIESFIRHEES S.lycopersicum LTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITSFDLSKVIESFIRHEES N.bentamiana LTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAFDLSKVIESFIRHEES N.tabacum LTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAFDLSKVIESFIRHEES V.vinifera LTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAFDLSKVIESFIRHEES G.max_isoX3 LTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAFDLSKVIESFIRHEES G.max_isoX1 LTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAFDLSKVIESFIRHEES G.max_isoX2 LTSLLTNERFHRCMLACSAELVLATHKTVTMLFPAVLERTGITAFDLSKVIESFIRHEES *. ** *******::****::******.: ::*****: :*:*:***.*:**.*:***:: S.bicolor_isoX1 LPRQLKRHLNSLEQQLLESMVWEKGSSLYNSLIVARPSVTSEIKRLGLLAEPMPSLDDLV S.bicolor_isoX3 LPRQLKRHLNSLEEQLLESMVWEKGSSLYNSLIVARPSIASEIKHLGLLAEPMPSLDDLV Z.mays_isoX1 LPRELKRHLNSLEEQLLESMAWEKGSSLYNSLIVARPSVASEINRLGLLAEPMPSLDDLV Z.mays_isoX3 LPRELKRHLNSLEEQLLESMAWEKGSSLYNSLIVARPSVASEINRLGLLAEPMPSLDDLV Z.mays_isoX2 LPRELKRHLNSLEEQILESMAWEKGSSLYNSLIVARPSVASEINRFGLLAESMPSLDDLV Z.mays_isoX5 LPRELKRHLNSLEEQILESMAWEKGSSLYNSLIVARPSVASEINRFGLLAESMPSLDDLV O.sativa_isoX2 LPRELKRHLNSLEEQLLESMSWEKGSSLYNSLVVARPSLSTEINSLGLLAEPMPSLDGIV O.vulgare_isoX1 LPRELKRHLNSLEEQLLESMAWEKGSSLYNSLIVARPSLASEINRLGLLAESMLSLDDIV T.aestivum_isoX3 LPRELKRHLNSLEEQLLESMAWEKGSSLYNSLVVARPSLAPEINRLGLLVESMPSLDDIV T.aestivum_isoX2 LPRELKRHLNSLEEQLLESMAWEKGSSLYNSLVVARPSLASEINRLGLLAESMPSLDDIV T.aestivum_isoX4 LPRELKRHLNSLEEQLLESMAWEKGSSLYNSLVVARPSLASEINRLGLLAESMPSLDDIV O.vulgare_isoX2 LPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPTLSLEINRLGLLAEPMPSLDAIA T.aestivum_isoX1 LPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPTLSAEINRLGLLAEPMPSLDAIA T.aestivum_isoX5 LPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPTLSAEINRLGLLAEPMPSLDAIA T.aestivum_isoX7 LPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPTLSAEINRLGLLAEPMPSLDAIA T.aestivum_isoX6 LPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPTLSAEINRLGLLAEPMPSLDAIA O.sativa_isoX1 LPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPTLSAEINRLGLLAEPMPSLDAIA Z.mays_isoX6 LPRELKRHLNSLEERLLESMSWEKGSSMYNSLIVARPVLSIEINRLGLLAEPMPSLDAIA S.bicolor_isoX2 LPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPALSVEINRLGLLAEPMPSLDAIA Z.mays_isoX4 LPRELKRHLNSLEERLLESMAWEKGSSMYNSLIVARPALSVEISRLGLLAEPMPSLDAIA A.thaliana LPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPSLALEINQLGLLAEPMPSLDAIA B.napus_isoX4 LPRELRRHLNSLEERLLESMVWERGSSMYNSLIVAKAPLALEINRLGLLAEPMPSLDAIA B.rapa_isoX3 LPRELRRHLNSLEERLLESMVWERGSSMYNSLIVAKAPLALEINRLGLLAEPMPSLDAIA B.rapa_isoX2 LPRELRRHLNSLEERLLESMVWERGSSMYNSLIVAKAPLALEINRLGLLAEPMPSLDAIA B.rapa_isoX4 LPRELRRHLNSLEERLLESMVWERGSSMYNSLIVAKAPLALEINRLGLLAEPMPSLDAIA B.napus_isoX3 LPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPLLALEINRLGLLAEPMPSLDAIA B.oleracea_isoX2 LPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPSLALEINRLGLLAEPMPSLDAIA B.oleracea_isoX1 LPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPSLALEINRLGLLAEPMPSLDAIA B.rapa_isoX1 LPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPLLALEINRLGLLAEPMPSLDAIA B.napus_isoX2 LPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPLLALEINRLGLLAEPMPSLDAIA B.napus_isoX1 LPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPLLALEINRLGLLAEPMPSLDAIA S.tuberosum LPRELRRHLNSLEERLLESMVWEKGSSMYNSLTVAKPSLSAEINRLGLLAEPMPSLDAIA S.pennellii LPRELRRHLNSLEERLLESMVWEKGSSMYNSLTVAKPSLSAEINRLGLLAEPMPSLDAIA S.lycopersicum LPRELRRHLNSLEERLLESMVWEKGSSMYNSLTVAKPSLSAEINRLGLLAEPMPSLDAIA N.bentamiana LPRELRRHLNSLEERLLESMVWEKGSSMYNSLAVAKPSLAAEINRMGLLAEPMPSLDAIA N.tabacum LPRELRRHLNSLEERLLESMVWEKGSSMYNSLAVAKPSLAAEINRTGLLAEPMPSLDAIA V.vinifera LPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARAALSAEVNRLGLLAEPMPSLDAIS G.max_isoX3 LPRELRRHLNSLEERLLESMVWEKGSSMYNSLAVARPSLSAEINRLGLLAEPMPSLDEIA G.max_isoX1 LPRELRRHLNSLEERLLESMVWEKGSSMHNSLAVARPSLSAEINSLGLLAEPMPSLDEIA G.max_isoX2 LPRELRRHLNSLEERLLESMVWEKGSSMYNSLAVARPSLSAEINRLGLLAEPMPSLDEIA ***:*.*******:.:**** **.***::*** **.. :: *:. ***.*.* *** : S.bicolor_isoX1 ARQNIHVEGLPATPS-KKRAAGP-DDNADPRSPKRSCNESRNTVVEHNLQTPPPKQSHMV S.bicolor_isoX3 ARQNIHVEGLPATPS-KKRDAGP-DDNADPRSPKRSCNESRNTVVEHNLLTPPPKH-HMV Z.mays_isoX1 SRQNVRIEGLPATPS-KKRAAGP-DDNADPRSPKRSCNESRNTVVERNLQTPPPKQSHMV Z.mays_isoX3 SRQNVRIEGLPATPS-KKRAAGP-DDNADPRSPKRSCNESRNTVVERNLQTPPPKQSHMV Z.mays_isoX2 ARQNIHIEGLPATPS-KKRAAGR-DDNADPRSPKRPCNESRSPVVEHNLQTPPPKQCHMV Z.mays_isoX5 ARQNIHIEGLPATPS-KKRAAGR-DDNADPRSPKRPCNESRSTVVEHNLQTPPPKQCHMV O.sativa_isoX2 ARQSIHPDGLPPTPSKRWPSAGP-DGNCYPQSPKRLCTESRNSLVERNSQTPPPKQSQTGO.vulgare_isoX1 ARQNFHAEDLPATPS-KKQAADP-DENGDTRSPKRLCIESRSTLVDQIPQTPSAKQSY--T.aestivum_isoX3 ARQNFHAEDLPATPS-KKRAADS-DENGDSRSPKRLCIEPRSTLVDQISQTPPAKQSY-- T.aestivum_isoX2 ARQNFHAEDLPATPS-KKRAAYS-DENGDSRSPKRLCIEPRSTLVDQISQTPPAKQSY-- T.aestivum_isoX4 ARQNFHAEDLPATPS-KKRAADS-DENGDSRSPKRLCIEPRSTLVDQISQTPPAKQSY-- O.vulgare_isoX2 VHHDISLGGLPPLPF-QKQERSP-GNKDEVRSPKRACTERRNVLAD-NSFRSPVKD----T.aestivum_isoX1 VHHDISLGGLPPLPF-HKQP-----DKDEVRSPKRACTERRNVLVD-NSFRSPVKD----T.aestivum_isoX5 VHHDISLGGLPPLPF-HKQP-----DKDEVRSPKRACTERRNVLVD-NSFRSPVKD---- T.aestivum_isoX7 VHHDISLGGLPPLPF-HKQP-----DKDEVRSPKRACTERRNVLVD-NSFRSPVKD---- T.aestivum_isoX6 VHHDISLGGLPPLPF-HKQP-----DKDEVRSPKRACTERRNVLVD-NSFRSPVKD---- O.sativa_isoX1 AHHNISLEGLPPLPF-QKQEHSP--DKDEVRSPKRACTERRNVLVDNNSFRSPVKD---- Z.mays_isoX6 AHHNISMGGLPPLPF-QKQERLQ--DKDEIRSPKRACTERRHVPVDSNSFRSQVKD---- S.bicolor_isoX2 AHHNISLGGLPPLPF-QKQDRLQ--DKDEVRSPKRACTERRNVLVDSNSFRSPVKD---- Z.mays_isoX4 AHHNISLGGLPPLPF-QKQERLQ--DKDEVRSPKRACTERRNVLVDSNSLRSPVKD---- A.thaliana ALINFSDGA-NHASSVQKHETCP-GQNGGIRSPKRLCTDYRSILVERNSFTSPVKDRLLA B.napus_isoX4 ALINFSEGSSNHASSVQKHEACP-GQNGDIKSPKRLCTDYRSILVERSSFASPVKERLLA B.rapa_isoX3 ALINFSEGSSNHASSVQKAEACP-GQNGDIKSPKRLCTDYRSILVERSSFASPVKERLLA B.rapa_isoX2 ALINFSEGSSNHASSVQKAEACP-GQNGDIKSPKRLCTDYRSILVERSSFASPVKERLLA B.rapa_isoX4 ALINFSEGSSNHASSVQKAEACP-GQNGDIKSPKRLCTDYRSILVERSSFASPVKERLLA B.napus_isoX3 ALINFSEGS-NHAPSIQKHETCP-GQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLA B.oleracea_isoX2 ALINFSEGS-HHAPSVQKHETCP-GQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLA B.oleracea_isoX1 ALINFSEGS-HHAPSVQKHETCP-GQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLA B.rapa_isoX1 ALINFSEGS-NHAPSIQKHETCP-GQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLA B.napus_isoX2 ALINFSEGS-NHAPSIQKHETCP-GQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLA B.napus_isoX1 ALINFSEGS-NHAPSIQKHETCP-GQNGDMKSPKRQCTDYRSILVERNSFTSPVKDRLLA S.tuberosum MHINLSSGNLPPVPPLQKSDLAPNGHNCDIRSPKRLCTEYRSVLVERNSFTSPVKDRFLA S.pennellii MHINPSLGNLPPVPPLQKSDLAPNGHICDIRSPKRLCTEYRSVLVERNSFTSPVKDRFLA S.lycopersicum MHINPSLGNLPPVPPLQKSDLAPNGHICDIRSPKRLCTEYRSVLVERNSFTSPVKDRFLA N.bentamiana MHINLSSGSLPPLPSLHKNNLAPNGQIGDIRSPKKVCSEYRSVLVERNSFTSPVKDRFLA N.tabacum MHINLSSGSLPPLPSLHKNNLAPNGQIGDIRSPKKVCSEYRSVLVERNSFTSPVKDRFLA V.vinifera MHINASCGGLPPVPSLQKRESSP-GQNGDIRSPKRVCPDYRSVLVERNSFTSPVKDRFLA G.max_isoX3 MHINFSCGGLPPVPTLPKLESSP-TQNGDIRSPK------RNVLMERNSFTSPVKDRLLP G.max_isoX1 MHINFSCGGLPPVPTLPKLESPS-NQNGDIRSPK------RNVLMERNSFTSPVKDCLLP G.max_isoX2 MHINFSCGGLPPVPTLPKLESSS-NQNGDIRSPK------RNVLMERNSFTSPVKDRLLP . . . .*** * : . * S.bicolor_isoX1 STSLKAKCHP--LQSTFESPTVSNPVGGNEKCADVTIKIFFSKILKLAAVRIRNLCERLQ S.bicolor_isoX3 STSLKAKCHP--LESTFASPTVSNPVGGNEKCADVAIQIFFSKILKLAAVRIRNLCERVQ Z.mays_isoX1 STSLKAKCHP--LQSTFASPTVCNPVGGNEKCADVTIHIFFSKILKLAAIRIRNLCERVQ Z.mays_isoX3 STSLKAKCHP--LQSTFASPTVCNPVGGNEKCADVTIHIFFSKILKLAAIRIRNLCERVQ Z.mays_isoX2 LTSLKAKCHP--LQSTFASPTVSNPVGGNEKCADVTIQIFFSKILKLAAIRIRNLCERIQ Z.mays_isoX5 LTSLKAKCHP--LQSTFASPTVSNPVGGNEKCADVTIQIFFSKILKLAAIRIRNLCERIQ O.sativa_isoX2 LSILKAKYHP--LQATFASPTVSNPVSGNEKCAVVGVQIFFSKILKLAAIRIRNLCERLR O.vulgare_isoX1 --TLKAKWCP--LQSTFASPTVSNPVGRNEKCAEVGVHIFFSKILKLAAIRIRNLCERLG T.aestivum_isoX3 --TLKAKWRP--LQSTFASPTVSNLVGGNEKCAEVGVHIFFSKILKLAAIRIRNLCERLR T.aestivum_isoX2 --TLKAKWRP--LQSTFASPTVSNPVGGNEKCAEVGVHIFFSKILKLAAIRIRNLCERLR T.aestivum_isoX4 --TLKAKWRP--LQSTFASPTVSNLVGGNEKCAEVGVHIFFSKILKLAAIRIRNLCERLR O.vulgare_isoX2 --AIKSKFLPP-LQSAFASPTRPNPAAGGETCAETGIGVFLSKITKLAAIRIKCLCERLQ T.aestivum_isoX1 --AIKSKFLPP-LQSAFASPTRPNPAAGGETCAETGIGVFLSKITKLAAIRIKCLCERLQ T.aestivum_isoX5 --AIKSKFLPP-LQSAFASPTRPNPAAGGETCAETGIGVFLSKITKLAAIRIKCLCERLQ T.aestivum_isoX7 --VIKSKFLPP-LQSAFASPTRPNPAAGGETCAETGIGVFLSKITKLAAIRIKCLCERLQ T.aestivum_isoX6 --VIKSKFLPP-LQSAFASPTRPNPAAGGETCAETGIGVFLSKITKLAAIRIKCLCERLQ O.sativa_isoX1 --TLKSKL-PP-LQSAFLSPTRPNPAAGGELCAETGIGVFLSKIAKLAAIRIRGLCERLQ Z.mays_isoX6 --SIKLKLPPP-LQSAFASPTRPNPAAGGETCAETGIAVFFSKISKLAAIRIRSLCERLQ S.bicolor_isoX2 --IIKSKLPPP-LQSAFASPTRPNPAAGGETCAETGIGVFFSKIAKLAAIRIRSLCERLQ Z.mays_isoX4 --IIKPKL-PP-LQSAFASPTRPNPAAGGETCAETGIGVFFSKISKLAAIRIRSLCERLQ A.thaliana LGNVKSKMLPPPLQSAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ B.napus_isoX4 LGNVKSKTLPPPLQSAFATPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ B.rapa_isoX3 LGNVKSKTLPPPLQSAFATPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ B.rapa_isoX2 LGNVKSKTLPPPLQSAFATPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ B.rapa_isoX4 LGNVKSKTLPPPLQSAFATPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ B.napus_isoX3 FGNVKSKMLPPPLQSAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ B.oleracea_isoX2 FGNVKSKMLPPPLQSAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ B.oleracea_isoX1 FGNVKSKMLPPPLQSAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ B.rapa_isoX1 FGNVKSKMLPPPLQSAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ B.napus_isoX2 FGNVKSKMLPPPLQSAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ B.napus_isoX1 FGNVKSKMLPPPLQSAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQ S.tuberosum LTNIKSKFPPP-LQSAFASPTRPNPGGGGETCAETSINVFFGKIVKLAAVRINGMIERLQ S.pennellii LTNIKSKFPPP-LQSAFASPTRPNPGGGGETCAETSINVFFGKIVKLAAVRINGMIERLQ S.lycopersicum LTNIKSKFPPP-LQSAFASPTRPNPGGGGETCAETSINVFFGKIVKLAAVRINGMIERLQN.bentamiana LNNIKSKFPPPALHSAFASPTRPNPGGGGETCAETAINVFFGKIVKLAAVRINGMIERLQN.tabacum LNNIKSKFPPPALQSAFASPTRPNPGGGGETCAETAINVFFGKIVKLAAVRINGMIERLQ V.vinifera LNNLKSKLPPPPLQSAFASPTRPNPGREGETCAETGINIFFSKIIKLAAVRINGMVERLQ G.max_isoX3 FNSLKSKLPPPPLQSAFASPTKPNPGGGGETCAETGINIFFGKIIKLGAVRISGMVERLQ G.max_isoX1 CIILKSKLPPPPLQSAFASPTKPNPGGGGETCAETGINIFFGKIIKLGAVRISGMVERLQG.max_isoX2 FNSLKSKLPPPPLQSAFASPTKPNPGGGGETCAETGINIFFGKIIKLGAVRISGMVERLQ:* * * * ::* :** * .* ** . : :*: ** **.*:** : **: S.bicolor_isoX1 HVELT-ERVYNVFNQILDQQTTLFFNRHIDQLILCCLYGVAKVCKLEPSFKELLNNYRKE S.bicolor_isoX3 HVELT-ERVYNVFNQILDQQTTLFFNRHIDQLILCCLYGVAKACKVELSFKELLNYYRKE Z.mays_isoX1 CVEQT-ERVYNVFKQILEQQTTLFFNRHIDQLILCCLYGVAKVCQLELTFREILNNYKRE Z.mays_isoX3 CVEQT-ERVYNVFKQILEQQTTLFFNRHIDQLILCCLYGVAKVCQLELTFREILNNYKRE Z.mays_isoX2 YMEQT-ERVYNVFKQILDQQTTLFFNRHIDQLILCCLYGVAKVCQLELSFREILNNYKKE Z.mays_isoX5 YMEQT-ERVYNVFKQILDQQTTLFFNRHIDQLILCCLYGVAKVCQLELSFREILNNYKKE O.sativa_isoX2 HEELT-VSVYNIFKQILDQQTALFFNRHVDQIILCCLYGVAKVSQLSLTFKEIVNNYKRE O.vulgare_isoX1 HVEQT-ERVYNIFKQILDQQTALFFNRHIDQLILCSLYGVAKVSQLTLTFKEIVNNYKRE T.aestivum_isoX3 HVEQT-ERVYNIFKQILDQQTTLFFNRHIDQLILCSLYGVAKVSQLTLTFKEIVNNYRRE T.aestivum_isoX2 HVEQT-ERVYNIFKQILDQQTTLFFNRHIDQLILCSLYGVAKVSQLTLTFKEIVNNYKRE T.aestivum_isoX4 HVEQT-EHVYNIFKQILDQQTTLFFNRHIDQLILCSLYGVAKVSQLTLTFKEIVNNYKRE O.vulgare_isoX2 LSQQILERVYSLAQQIISQQTALFFNRHIDQIILCCIYGVAKISQLALTFKEIIFSYRKQ T.aestivum_isoX1 LSQQILERVYSLVQQIISQQTALFFNRHIDQIILCCIYGVAKISQLALTFKEIIFSYRKQ T.aestivum_isoX5 LSQQILERVYSLVQQIISQQTALFFNRHIDQIILCCIYGVAKISQLALTFKEIIFSYRKQ T.aestivum_isoX7 LSQQILERVYSLVQQIISQQTALFFNRHIDQIILCCIYGVAKISQLALTFKEIIFSYRKQ T.aestivum_isoX6 LSQQILERVYSLVQQIISQQTALFFNRHIDQIILCCIYGVAKISQLALTFKEIIFSYRKQ O.sativa_isoX1 LSQQVLERVYSLVQQIIIQQTALFFNRHIDQIILCSIYGVAKISQLALTFKEIIFGYRKQ Z.mays_isoX6 LSQQVLERVYSLVQQILSQQTGLFFNRHIDQIILCSIYGVAKISQLQLSFKEIVFGYRKQ S.bicolor_isoX2 LSQQVLERVYSLVQQILSQQTGLFFNRHIDQIILCSIYGVAKISQLELSFKEIIFGYRKQ Z.mays_isoX4 LPQQVLERVYSLVQQILSQQTGLFFNRHIDQIILCSIYGVAKISQLELSFKEIIFGYRKQ A.thaliana LSQQIRESVYCFFQHVLAQRTSLLFSRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ B.napus_isoX4 LSQQIRESVYCLFQRVLAQRTSLLFNRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ B.rapa_isoX3 LSQQIRESVYCLFQRVLAQRTSLLFNRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ B.rapa_isoX2 LSQQIRESVYCLFQRVLAQRTSLLFNRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ B.rapa_isoX4 LSQQIRESVYCLFQRVLAQRTSLLFNRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ B.napus_isoX3 LSQQIRESVYRLFQHVLAQRTSLLFNRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ B.oleracea_isoX2 LSQQIRESVYRLFQHVLAQRTSLLFNRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ B.oleracea_isoX1 LSQQIRESVYRLFQHVLAQRTSLLFNRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ B.rapa_isoX1 LSQQIRESVYRLFQHVLAQRTSLLFNRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ B.napus_isoX2 LSQQIRESVYRLFQHVLAQRTSLLFNRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ B.napus_isoX1 LSQQIRESVYRLFQHVLAQRTSLLFNRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQ S.tuberosum LSQQIRETVYCLFQKILNQRTSLFFSRHIDQIILCSFYGVAKISQLNLTFKEIIYNYRKQ S.pennellii LSQQIRETVYCLFQKILSQRTSLFFSRHIDQIILCSFYGVAKISQLNLTFKEIIYNYRKQ S.lycopersicum LSQQIRETVYCLFQKILSQRTSLFFSRHIDQIILCSFYGVAKISQLNLTFKEIIYNYRKQ N.bentamiana LSQQIRETVYCLFQKILSQRTSLFFNRHIDQIILCSFYGVAKISQLNLTFKEIICNYRKQ N.tabacum LSQQIRETVYCLFQKILSQRTSLFFNRHIDQIILCSFYGVAKISQLNLTFKEIICNYRKQ V.vinifera LSQQMRENVYCLFQQILNRRTSLFFNRHIDQIILCCFYGLAKISQMNLTFREIIHNYRKQ G.max_isoX3 LSQQIRENVYCLFQRILNQWTSLFFNHHIDQIILCCFYGVAKISQLNLTFKEIVYNYRKQ G.max_isoX1 LSQQIRENVYSLFQRILNQWTSLFFNRHIDQIILCCFYGVAKISQLNLTFKEIVYNYRKQ G.max_isoX2 LSQQIRENVYSLFQRILNQWTSLFFNRHIDQIILCCFYGVAKISQLNLTFKEIVYNYRKQ : ** . :.:: ..* *:*..*:**:***.:**:** .:: :*.*:: *..: S.bicolor_isoX1 AQCKPEVFSNVYIG--SRNRND--VLVSRHVDIITFYNEVFVPAAKSFLVSLIS--SGTR S.bicolor_isoX3 AQCKPEVFQNIYIG--SRNSND--VLVSRHVSIISFYNAVFVPAAKPFLVSLIS--SGTC Z.mays_isoX1 AQCKPEVFSSIYIG--STNRNG--VLVSRHVGIITFYNEVFVPAAKPFLVSLIS--SGTH Z.mays_isoX3 AQCKPEVFSSIYIG--STNRNG--VLVSRHVGIITFYNEVFVPAAKPFLVSLIS--SGTH Z.mays_isoX2 AQCKPEVFLSIYIG--SRNHNG--VLISRHVDIITFYNEVFVPAAKPFLVSLIS--SGTR Z.mays_isoX5 AQCKPEVFLSIYIG--SRNHNG--VLISRHVDIITFYNEVFVPAAKPFLVSLIS--SGTR O.sativa_isoX2 PQCKPEVFRSIFVG--STNRNG--GFGSRHVDIIVFYNQVFVPTVKPLLVALMP--SSTR O.vulgare_isoX1 QQCMPEVFRSVFVV--NTDHNG--GLGSRHVDIIIFYNEVFVPAVKPFLVALIP--SGAH T.aestivum_isoX3 QQCIPEVFRSVFVV--NTNHNG--GLGSHHVDIIVFYNEVFVPAVKPFLVALIP-SGGAH T.aestivum_isoX2 QQCIPEVFRSVFVV--NTNHNG--GLGSRHVDIIVFYNEVFVPAIKPFLVALIP-SGGAH T.aestivum_isoX4 QQCIPEVFRSVFVV--NTNHNG--GLGSRHVDIIVFYNEVFVPAVKPFLVALIP-SGGAH O.vulgare_isoX2 SQCKPQVFRSVYVNWPSRSRSG--KIGEDHVDIITFYNEVFIPTVKPLLVDLGP---GTS T.aestivum_isoX1 SQCKPQVFRSVYVNWPSRSRSG--KIGEDHVDIITFYNEVFIPTVKPLLVDLGP---GTS T.aestivum_isoX5 SQCKPQVFRSVYVNWPSRSRSG--KIGEDHVDIITFYNEVFIPTVKPLLVDLGP---GTS T.aestivum_isoX7 SQCKPQVFRSVYVNWPSRSRSG--KIGEDHVDIITFYNEVFIPTVKPLLVDLGP---GTS T.aestivum_isoX6 SQCKPQVFRSVYVNWPSRSRSG--KIGEDHVDIITFYNEVFIPTVKPLLVDLGP---GTS O.sativa_isoX1 SQCKPQVFRSVYVHWASRSRNG--KTGEDHVDIITFYNEVFIPTVKPLLVELGS---GTS Z.mays_isoX6 PQCKPQVFRSVYVHWPPTSHNG--KTGEDHVDIITFYNEVFIPAVKPLLVEVGP---GAS S.bicolor_isoX2 PQCKPQVFRSVYVHWPPRSRNG--KTGEDHVDIITFYNEVFIPAVKPLLVEVGP---GASZ.mays_isoX4 PQCKPQVFRSVYVHWPPRSRNG--KTGEDHVDIITFYNEVFIPAVKSLLVEVGP-GASASA.thaliana PQCKPLVFRSVYVDALQCRRQG--RIGPDHVDIITFYNEIFIPAVKPLLVELGPVRNDRA B.napus_isoX4 PQCKPLVFRSVFVDSSQGRRQG--RVGPDHVDIITFYNDVFIPAVKPLLVEIVPVKKGQA B.rapa_isoX3 PQCKPLVFRSVFVDSSQGRRQG--RVGPDHVDIITFYNDVFIPAVKPLLVEIVPVKKGQA B.rapa_isoX2 PQCKPLVFRSVFVDSSQGRRQG--RVGPDHVDIITFYNDVFIPAVKPLLVEIVPVKKGQAB.rapa_isoX4 PQCKPLVFRSVFVDSSQGRRQG--RVGPDHVDIITFYNDVFIPAVKPLLVEIVPVKKGQAB.napus_isoX3 PQCKPLVFRSVYVDSHQSRRQGQGRVGPDHVDIITFYNEIFIPAVKPLLVEIIPVKKDQA B.oleracea_isoX2 PQCKPLVFRSVYVDSHQSRRQGQWRVGPDHVDIITFYNEIFIPAVKPLLVEIIPVKKDQA B.oleracea_isoX1 PQCKPLVFRSVYVDSHQSRRQGQWRVGPDHVDIITFYNEIFIPAVKPLLVEIIPVKKDQA B.rapa_isoX1 PQCKPLVFRSVYVDSHQSRRQGQGRVGPDHVDIITFYNEIFIPAVKPLLVEIIPVKKDQA B.napus_isoX2 PQCKPLVFRSVYVDSHQSRRQGQGRVGPDHVDIITFYNEIFIPAVKPLLVEIIPVKKDQA B.napus_isoX1 PQCKPLVFRSVYVDSHQSRRQGQGRVGPDHVDIITFYNEIFIPAVKPLLVEIIPVKKDQA S.tuberosum PQCKPQVFRSVFVDWTSARHNG--KTGSEHVDIITFYNEMFIPSVKPLLVELAP--AGNE S.pennellii PQCKPQVFRSVFVDWTSARHNG--KTGSEHVDIITFYNEMFIPSVKPLLVELAP--AGNE S.lycopersicum PQCKPQVFRSVFVDWTSARHNG--KTGSEHVDIITFYNEMFIPSVKPLLVELAP--AGNE N.bentamiana PQCKPQVFRSVFVDWTLARHNV--RTGADHVDIITFYNEMFIPSVKPLLVELAP--AGNN N.tabacum PQCKPQVFRSVFVDWTLARHNV--RTGADHVDIITFYNEMFIPSVKPLLVELAP--AGNA V.vinifera PQCKPQIFRSVFVDWSSARRNG--KTGKEHVDIITFYNEIFIPAVKPLLMEIGP-GGGTT G.max_isoX3 PHCKTQVFRSVFVDWSLARRNG--RTGQDHVDIITFYNEIFIPSVKPLLVELGP--AGPT G.max_isoX1 PHCKPQVFRSVFVDWSLARRNG--RTGQEHIDIITFYNEIFIPSVKPLLVELGP--AGPT G.max_isoX2 PHCKPQVFRSVFVDWSLARRNG--RTGQEHIDIITFYNEIFIPSVKPLLVELGP--AGPT :* . :* .::: . *:.** *** :*:*: *.:*: : . . S.bicolor_isoX1 PEDKKNAS----------GQI---PGSPKPSPLP-NLPDLSPKKVSASHNVYVSPLQQAK S.bicolor_isoX3 PEDKKNAS----------GPI---PGSPKPSPLPNNLPDMSPKKVSASHNVYVSPLRQTK Z.mays_isoX1 PEDKKNAS----------GQI---PGSPKPSPFP-NLPDMSPKKVSASHNVYVSPLRQTK Z.mays_isoX3 PEDKKNAS----------GQI---PGSPKPSPFP-NLPDMSPKKVSASHNVYVSPLRQTK Z.mays_isoX2 PEDKKNAS----------GQV---PGSPKLSPFP-NLPDMSPKKVSASHNVYVSPLRQTK Z.mays_isoX5 PEDKKNAS----------GQV---PGSPKLSPFP-NLPDMSPKKVSASHNVYVSPLRQTK O.sativa_isoX2 PEDKRNTN----------SQI---PGSPKSSPFS-NLPDMSPKKVSSSHNVYVSPLRQTK O.vulgare_isoX1 PDNKKNHN----------SQI---PGSPKSSPFS-NLPDMSPKKVSSSHNVYISPLRQTK T.aestivum_isoX3 PDNKKNPN----------SQI---SGSPKPSPFS-NLPDMSPKKVSSSHNVYISPLRQTK T.aestivum_isoX2 PDNKKNPN----------SQI---PGSPKSPPFS-NLPDMSPKKVSSSHNVYISPLRQTK T.aestivum_isoX4 PDNKKNPN----------SQI---PGSPKSPPFS-NLPDMSPKKVSSSHNVYISPLRQTK O.vulgare_isoX2 PNRNNEPKSGGD------APF---PESPRLSRFP-NLPDMSPKKVSATHNVYVSPLRSSK T.aestivum_isoX1 PNRNNEPKSG--------GDAASFPESPRLSRFP-NLPDMSPKKVSATHNVYVSPLRSSK T.aestivum_isoX5 PNRNNEPKSG--------GDAASFPESPRLSRFP-NLPDMSPKKVSATHNVYVSPLRSSK T.aestivum_isoX7 PNRNNEPKSGGDVFVFFVTSAAPFPESPRLSRFP-NLPDMSPKKVSATHNVYVSPLRSSK T.aestivum_isoX6 PNRNNEPKSGGD-----AAPF---PESPRLSRFP-NLPDMSPKKVSATHNVYVSPLRSSK O.sativa_isoX1 PNKKNEEKCAAD------GPY---PESPRLSRFP-NLPDMSPKKVSAAHNVYVSPLRTSK Z.mays_isoX6 PKRKEEGKGSVYV-----GPY---PESPRLARFP-NLPDMSPKKVSATHNVYVSPLRSSK S.bicolor_isoX2 PKKKEEEKGSADV-----GPY---PESPRLARFP-NLPDMSPKKVSATHNVYVSPLRSSK Z.mays_isoX4 PKKKEEEKGPAD-----VGPF---PESPRLARFP-NLPDMSPKKVSATHNVYVSPLRSSK A.thaliana VEANNKPE----------GQC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK B.napus_isoX4 VEANNNPE----------GHC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK B.rapa_isoX3 VEANNNPE----------GHC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK B.rapa_isoX2 VEANNNPE----------GHC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK B.rapa_isoX4 VEANNNPE----------GHC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK B.napus_isoX3 MEANNKPE----------GHC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK B.oleracea_isoX2 MEANNKAE----------GHC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK B.oleracea_isoX1 MEANNKAE----------GHC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK B.rapa_isoX1 MEANNKPE----------GHC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK B.napus_isoX2 MEANNKPE----------GHC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK B.napus_isoX1 MEANNKPE----------GHC---PGSPKVSVFP-SVPDMSPKKVSAVHNVYVSPLRGSK S.tuberosum QKNNHVEKTKND------GQG---PASPRPSSFP-SLPDMSPKKVSAVHNVYVSPLRSSK S.pennellii QKNNHVEKTKND------GQG---PASPRPSSFP-SLPDMSPKKVSAVHNVYVSPLRSSK S.lycopersicum QKNNHVEKTKKD------GQG---PASPRSSSFP-SLPDMSPKKVSAVHNVYVSPLRSSK N.bentamiana SEKNDHND----------GQG---PASPKPSPFP-KLPDMSPKKVSAVHNVYVSPLRASK N.tabacum EKNNHND-----------GQG---PASPKPSPFP-KLPDMSPKKVSAVHNVYVSPLRASK V.vinifera TKTNRVPEVNNN-ND---AQC---PESPKISPFP-SLPDMSPKKVSAAHNVYVSPLRSSK G.max_isoX3 PKSDRIPEVNNK-NEGHLAQC---PGSPKISPFP-TLPDMSPKKVSATHNVYVSPLRSSK G.max_isoX1 TKSDRIPEVNKN--DGHLAQC---PGSPKISPFP-TLPDMSPKKVSATHNVYVSPLRSSK G.max_isoX2 TKSDRIPEINKN--DGHLAQC---PGSPKISPFP-TLPDMSPKKVSATHNVYVSPLRSSK . . . **. . :. .:**:******: ****:***. :* S.bicolor_isoX1 ------------------------------MDLLLSPSSRSFYACFGEGTHAYQSPSKDL S.bicolor_isoX3 ------------------------------MDALLSPSSRSFYACIGEGTHAYQSPSKDL Z.mays_isoX1 ------------------------------LDLLLSPSSRSFYACIGEGTHAYQSPSKDL Z.mays_isoX3 ------------------------------LDLLLSPSSRSFYACIGEGTHAYQSPSKDLZ.mays_isoX2 ------------------------------MDLLLSPSSRSFYACIGEGTHAYQSPSKDLZ.mays_isoX5 INMAYACLNSTAQYVSTTASMTKLLSPKLGMDLLLSPSSRSFYACIGEGTHAYQSPSKDL O.sativa_isoX2 ------------------------------MDALLSPSSRSFYACIGESTQAFQSPSKDL O.vulgare_isoX1 ------------------------------KDALLSPSSRSFYACIGESTHAYQSSSEDL T.aestivum_isoX3 ------------------------------KDALLSPSSRSFYACIGESTHAYQSPSEDLT.aestivum_isoX2 ------------------------------KDALLSPSSRSFYACIGESTHAYQSPSEDLT.aestivum_isoX4 ------------------------------KDALLSPSSRSFYACIGESTHAYQSPSEDL O.vulgare_isoX2 ------------------------------MDTLLSPSSKSYYACVGESTHAFQSPSKDL T.aestivum_isoX1 ------------------------------MDTLLSPSSKSYYACVGESTHAFQSPSKDL T.aestivum_isoX5 ------------------------------MDTLLSPSSKSYYACVGESTHAFQSPSKDL T.aestivum_isoX7 ------------------------------MDTLLSPSSKSYYACVGESTHAFQSPSKDL T.aestivum_isoX6 ------------------------------MDTLLSPSSKSYYACVGESTHAFQSPSKDL O.sativa_isoX1 ------------------------------MDTLLSPSSKSYYACVGESTHAFQSPSKDL Z.mays_isoX6 ------------------------------MDTLLSPSPKSYYACVGESTYAFQSPSKDL S.bicolor_isoX2 ------------------------------MDTLLSPSSKSYYACVGESTYAFQSPSKDL Z.mays_isoX4 ------------------------------MDTLLSPSSKSYYACVGESTYAFQSPSKDL S.bicolor_isoX1 AAINSCLNY--SGRRLSSRLNF------DMVSDSVVAGSLGQ----------------PN S.bicolor_isoX3 AAINSRLNY--SGRRINSRLNF------DMVSDSVVAGSLGQ----------------PN Z.mays_isoX1 AAINSRLNY--NGRKVNSRLNF------DMVSDSVVAGSLGQ----------------IN Z.mays_isoX3 AAINSRLNY--NGRKVNSRLNF------DMVSDSVVAGSLGQ----------------IN Z.mays_isoX2 AAINSRLNY--NGRRVNSRLNF------DMVSDSVVAGSLGQ----------------PN Z.mays_isoX5 AAINSRLNY--NGRRVNSRLNF------DMVSDSVVAGSLGQ----------------PN O.sativa_isoX2 AAINSRLNY--PTRRINTRINF------DMVSDSVVAGSLGQ----------------PN O.vulgare_isoX1 AAINNCLNY--TTRRINTRINF------DMVSDSVVAGSLGQ----------------PN T.aestivum_isoX3 AAINNCLNY--TSRRINTRINF------DMVSDSVVAGSLGQ----------------PN T.aestivum_isoX2 AAINNCLNY--TSRRINTRINF------DMVSDSVVAGSLGQ----------------PN T.aestivum_isoX4 AAVNNCLNY--TSRRINTRINF------DMVSDSVVAGSLGQ----------------PH O.vulgare_isoX2 NAINTRLNS---GKKVNGRLNF------DVVSDLVVARSLSD----------------QN T.aestivum_isoX1 NAINTRLNS---GKKVNGRLNF------DVVSDLVVARSLSD----------------QN T.aestivum_isoX5 NAINTRLNS---GKKVNGRLNF------DVVSDLVVARSLSD----------------QN T.aestivum_isoX7 NAINTRLNS---GKKVNGRLNF------DVVSDLVVARSLSD----------------QN T.aestivum_isoX6 NAINTRLNS---GKKVNGRLNF------DVVSDLVVARSLSD----------------QN O.sativa_isoX1 KVINNRLNS---GKKVSGRLNF------DVVSDLVVARSLSD----------------QN Z.mays_isoX6 KAINNRLNS---WKKVSGRLNF------DIVSDLVVASSLNG----------------VQ S.bicolor_isoX2 KAINNRLNSVSSGKKVNGRLNF------DVVSDLVVASSLSS----------------DQ Z.mays_isoX4 KAINNRLNSVSGGKKVSGRLNF------DVVSDLVVASSLGS----------------DR A.thaliana SAINNRLNN--SSSNRKRTLNF-DAEA-GMVSDSMVANSLNLQNQN----------QNQN B.napus_isoX4 SAINNRLNNSNNSSNRKRTLKF-DVEA-GLVSDSMVGNSLYQ--------------QNQN B.rapa_isoX3 SAINNRLNN----SNRKRTLKF-DVEA-GLVSDSMVGNSLYQQNQNQNQNETDASNQNQN B.rapa_isoX2 SAINNRLNN-NNSSNRKRTLKF-DVEA-GLVSDSMVGNSLYQQNQNQNQNETDASNQNQN B.rapa_isoX4 SAINNRLNN-----NRKRTLKF-DVEA-GLVSDSMVGNSLYQQNQNQNQNETDASNQNQN B.napus_isoX3 SAINNRLNK------------Y-ISFC-GIAAAAPTARGRLNLT------------RKQD B.oleracea_isoX2 SAINNRLNN--CSTNRKRTLKF-DVEA-GLVSDSMVGNSLYL--------------QNQN B.oleracea_isoX1 SAINNRLNN-SCSTNRKRTLKF-DVEA-GLVSDSMVGNSLYL--------------QNQN B.rapa_isoX1 SAINNRLNN-SCSTNRKRTLKF-DAEA-GLVSDSMVGNSLYLQN------------QNQN B.napus_isoX2 SAINNRLNN--CSTNRKRTLKF-DAEA-GLVSDSMVGNSLYLQN------------QNQN B.napus_isoX1 SAINNRLNN-SCSTNRKRTLKF-DAEA-GLVSDSMVGNSLYLQN------------QNQNS.tuberosum TVINNRLNG---NRKLRGALNFDDVDAVGLVSDSIVANTLYL----------------QNS.pennellii TVINNRLNG---NRKLRGALNFDDVDAVGLVSDSIVANTLYL----------------QN S.lycopersicum TVINNRLNG---NRKLRGALNFDDVDAVGLVSDSIVANTLYL----------------QN N.bentamiana DVINNRLNG---NRKLRGALNF-DVDA-GLVSDSIVANSLYL----------------QN N.tabacum DVINNRLNG---NRKLRGALNF-DVDA-GLVSDSIVANSLYL----------------QNV.vinifera TAINNRLNS---TRKLRGALNFDDVE--GLVSDSLVAKSLYL----------------QNG.max_isoX3 TAINNRLNG---NRKVRGPLNFDDVDV-GLVSDSMVANSLYL----------------QN G.max_isoX1 TAINNRLNG---NRKVRGPLNFDDVDF-GLVSDSMVANSLYL----------------QN G.max_isoX2 TAINNRLNG---NRKVRGPLNFDDVDI-GLVSDSMVANSLYL----------------QN .:*. ** : .:.: .. S.bicolor_isoX1 GGSTSLDPAAAF--SPISKKREPDT S.bicolor_isoX3 GGSTSFNPAAAFSPLSKKRKPDDQI Z.mays_isoX1 GGSTSDPAAAF---SPLSKKRETDT Z.mays_isoX3 GGSTSDPAAAF---SPLSKKRETDT Z.mays_isoX2 GGSTSLDPAAAF--SPLSKRKPDT- Z.mays_isoX5 GGSTSLDPAAAF--SPLSKRKPDT- O.sativa_isoX2 GGSASSDPAAAF--SPLSKKSKTDS O.vulgare_isoX1 GVPASSDPAGSFSFLSRKGNPGPDS T.aestivum_isoX3 GVPASSDPAGSFSFLSRKDNPGPDS T.aestivum_isoX2 GVPASSDPAGSFSFLSRKDNSGPDS T.aestivum_isoX4 GVPASSDPAGSFSFLSRKDNPGPDS O.vulgare_isoX2 DGSASAMAVFGTK-TPVKGEQPDP- T.aestivum_isoX1 GSSAAAMAVFGTK-TPVKGEQQDP- T.aestivum_isoX5 GSSAAAMAVFGTK-TPVKGEQQDP- T.aestivum_isoX7 GGSAAAMAVFGTR-TPVKGEQQDP- T.aestivum_isoX6 GGSAAAMAVFGTK-TPVKGEQQDP- O.sativa_isoX1 SASAAATTADIATKTPVKLEQPDC- Z.mays_isoX6 NAKPAATEVVPTK-TPVKCEPSES- S.bicolor_isoX2 NAKPAATEVVPIK-TPVKCEPSDS- Z.mays_isoX4 DAKPAADPAKT---TPVKCEPSDS- A.thaliana GSDASSSGGA----APLKTEPTDS- B.napus_isoX4 GSDASSSGG-----APFKTEPQD-- B.rapa_isoX3 GSDASSSGG-----APFKTEPQD-- B.rapa_isoX2 GSDASSSGG-----APFKTEPQD-- B.rapa_isoX4 GSDASSSGG-----APFKTEPQD-- B.napus_isoX3 WSV-----------IPW-------- B.oleracea_isoX2 GSDASSSGG-----APLKTEPVDSS B.oleracea_isoX1 GSDASSSGG-----APLKTEPVDSS B.rapa_isoX1 GSDASSSGG-----APLKTEPVDSS B.napus_isoX2 GSDASSSGG-----APLKTEPVDSS B.napus_isoX1 GSDASSSGG-----APLKTEPVDSS S.tuberosum GNCISSPR------AAVKTEQPES- S.pennellii GNCISSPR------AAVKTEQPEP- S.lycopersicum GNCISSPR------AAVKTEQPEP- N.bentamiana GNCRSPV-------AHVKTEQPES- N.tabacum GNCRSPV-------AHVKTEQPES- V.vinifera GSCASSSG------APLKLEQPDT- G.max_isoX3 GSCASSSG------APLKSEQPDS- G.max_isoX1 GSCASSSG------APFKSEQPDS- G.max_isoX2 GSCASSSG------APLKSEQPDS-

Claims

Claims 1. A method for facilitating endosperm development in an angiosperm central cell a sperm cell, comprising inhibiting or substantially decreasing expression or activity, or lowering the concentration, of RBR1 (RETINOBLASTOMA RELATED1) in said central cell.

2. The method according to claim 1, wherein inhibiting or substantially decreasing expression or activity, or lowering the concentration, of RBR1 comprises expression of an agent targeting RBR1, particularly wherein said agent is selected from a. a nucleic acid agent capable of interfering with RBR1 expression; b. a polypeptide agent capable of degrading RBR1.

3. The method according to claim 2, wherein the agent targeting RBR1 is a ribonucleic acid capable of hybridizing to an mRNA transcribed from the rbr1 gene, particularly wherein the ribonucleic acid capable of hybridizing to an mRNA transcribed from the rbr1 gene is selected from the group consisting of a miRNA and a siRNA.

4. The method according to claim 2, wherein said agent targeting RBR1 is a polypeptide agent capable of degrading RBR1.

5. The method according to claim 4, wherein said angiosperm central cell comprises a recombinant copy of the rbr1 gene encoding a variant of RBR1, and said polypeptide agent is specifically capable of a. degrading said variant directly or b. inducing degradation of the variant by an intracellular degradation pathway, particularly by ubiquitinylation 6. The method according to any one of the preceding claims 2 to 5, wherein expression of said agent targeting RBR1 is controlled by a promoter specifically active in said central cell.

7. The method according to any one of the preceding claims, wherein the central cell is a cell of a plant comprised in the family Poaceae (grasses); particularly wherein the central cell is a cell of a plant comprised in a genus comprised in the group consisting of Triticum (wheat), Hordeum (barley); Secale (rye); Zea (maize); Oryza (rice); Avena.

8. A recombinant angiosperm cell, comprising a transgene encoding a nucleic acid agent targeting RBR1 under transcriptional control of a promoter sequence specifically operable in an angiosperm central cell; particularly wherein said nucleic acid agent is a miRNA or siRNA targeting the RBR1 encoding mRNA.

9. A recombinant angiosperm cell, comprising a transgene encoding a polypeptide specifically leading to degradation of RBR1, said transgene being under transcriptional control of a promoter sequence specifically operable in an angiosperm central cell.

10. The recombinant cell of claim 9, wherein the polypeptide specifically leading to degradation of RBR1 is an antibody-like ligand specifically targeting RBR1.

11. The recombinant cell of claim 9, wherein a. The cell comprises a transgene encoding a variant of RBR1 having RBR1 biological activity, the variant being specifically cleavable by a protease; b. the polypeptide specifically leading to degradation of RBR1 is a protease capable of specifically cleaving said variant.

12. The recombinant cell according to any one of claims 8 to 11, wherein the promoter sequence is selected from the group consisting of pZmEBE, pMEA, pDME, pRALF18, pAGL80, pDD46, pDIA / pAGL61, pFIE.

13. The recombinant cell according to any one of claims 8 to 12, wherein the cell is a plant cell of a plant comprised in the family Poaceae (grasses) and the RBR1, or its orthologue, or its variant, is the natural RBR1 orthologue of the plant.

14. A plant comprising an angiosperm cell according to any one of claims 8 to13.

15. A seed of a plant according to claim 14, capable of development of endosperm in absence of fertilization.

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