Methods of treatment using agents binding to epcam and CD137 in combination with PD-1 axis binding antagonists

Abstract

The present disclosure provides for the use of a binding agent that binds to EpCAM and to CD137 in combination with a PD-1 axis binding antagonist in the treatment or prevention of a tumor or cancer, or to prevent progression of a tumor or cancer.

Description

[0001] METHODS OF TREATMENT USING AGENTS BINDING TO

[0002] EPCAM AND CD137 IN COMBINATION WITH PD-1 AXIS BINDING ANTAGONISTS

[0003] Technical Field

[0004] The present disclosure relates to binding agents that bind to EpCAM and to CD137 and to PD-1 axis binding antagonists. Together, they are useful to treat or prevent a tumor or cancer.

[0005] Background

[0006] Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-Myc, E-FABP, and cyclins A & E. EpCAM can be used as diagnostic marker for various cancers. Also, EpCAM appears to play a role in tumorigenesis and metastasis of carcinomas, and, thus, it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.

[0007] CD137 (4-1BB) is a member of the TNFR family and is a co-stimulatory molecule on CD8+ and CD4+ T cells, regulatory T cells (Tregs), Natural Killer (T) cells (NK[T] cells), B cells and neutrophils. On T cells, CD137 is not constitutively expressed, but induced upon T-cell receptor (TCR) activation (for example, on tumor infiltrating lymphocytes [TILs; Gros et al., J. Clin Invest 2014;124(5):2246-59]). Stimulation via its natural ligand 4-1BBL or agonist antibodies leads to signaling using TRAF-2 and TRAF-1 as adaptors. Early signaling by CD137 involves K63 poly-ubiquitination reactions that ultimately result in activation of the nuclear factor (NF)-KB and mitogen-activated protein (MAP)-kinase pathways. Signaling leads to increased T-cell co-stimulation, proliferation, cytokine production, maturation and prolonged CD8+ T-cell survival. Agonistic antibodies against CD137 have been shown to promote anti-tumor control by T cells in various pre-clinical models (Murillo et al., Clin Cancer Res 2008;14(21):6895-906). Antibodies stimulating CD137 can induce survival and proliferation of T cells, thereby enhancing the anti-tumor immune response. Antibodies stimulating CD137 have been disclosed in the prior art, and include urelumab, a human IgG4 antibody (AU 2004279877) and utomilumab, a human IgG2 antibody (Fisher et ai., 2012, Cancer Immunol. Immunother. 61: 1721-1733).

[0008] PD-1 and its ligands PD-L1 and PD-L2 are inhibitory checkpoint molecules regulating the immune system and enabling self-tolerance. At the same time inhibitory checkpoint molecules are ideal targets for cancer immunotherapy.

[0009] In tumor-draining lymph nodes and within the tumor microenvironment, 4-1BB is expressed by a subset of CD4+ and CD8+ T cells that are characterized by the co-expression of multiple TCR-inducible molecules including high levels of programmed cell death 1 (PD-1) (Gros et al., J. Clin Invest 2014;124(5):2246-59; Seifert et al., Cancers (Basel) 12; Simoni et al., Nature 557: 575-579). Upregulation of PD-1 on T cells can contribute to T-cell exhaustion and reduce T-cell activation upon binding to its ligand programmed cell death 1 ligand 1 (PD-L1) (Yu et al., Eur J Pharmacol 881: 173240). PD-L1 expression is often upregulated by tumor cells, particularly in inflamed tumors (Teng, et al., Cancer Res 75: 2139-2145). Thereby, the tumor cells provide an inhibitory signal to activated T cells through which they can evade T-cell mediated cytotoxicity. Antibodies that block the PD-1 / PD-L1 inhibitory axis can restore T-cell function (Boussiotis et al., N Engl J Med 375: 1767-1778; Chen et al., Nature 541: 321-330). However, despite these advances in the art there is a considerable need for improved therapies to prevent progression of a tumor or treating cancer.

[0010] Summary

[0011] The present inventors have surprisingly found that a combination of (i) a binding agent comprising a first antigen- binding region binding to EpCAM and a second antigen-binding region binding to CD137, and (ii) a PD-1 axis binding antagonist (in particular inhibition of the PD-1 / PD-L1 axis) enhanced cytotoxic activity of activated antigen-specific human CD8+T cells towards cognate antigen-expressing tumor cells compared to either component in the combination alone.

[0012] In a first aspect, the present disclosure provides a method for treating or preventing a disease or disorder in a subject, said method comprising (i) administering to the subject a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137, and (ii) administering to the subject a PD-1 axis binding antagonist, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD-1 axis binding antagonist.

[0013] In some embodiments, simultaneous administration refers to administration of the binding agent and the PD-1 axis binding antagonist at the same time or within minutes or within a few hours of each other, e.g., 5, 10, 15, 20, 30, 45 minutes or 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 18 hours.

[0014] In some embodiments, administration prior to refers to administration of the binding agent at least a day prior to the administration of the PD-1 axis binding antagonist, e.g., 1, 2, 3, 4, 5, 6 or 7 days prior, or at least 1, 2, 3, 4, 5,

[0015] 6, 7, 8 weeks prior, or at least 1, 2, 3, 4, 5, or 6 months prior.

[0016] In some embodiments, administration after to refers to administration of the binding agent at least a day after the administration of the PD-1 axis binding antagonist, e.g., 1, 2, 3, 4, 5, 6 or 7 days after, or at least 1, 2, 3, 4, 5, 6,

[0017] 7, 8 weeks after, or at least 1, 2, 3, 4, 5, or 6 months after.

[0018] In some embodiments, the administration of the binding agent is prior to or after a treatment cycle of the PD-1 axis binding antagonist. In some embodiments, the administration of the PD-1 axis binding antagonist is prior to or after a treatment cycle of the binding agent.

[0019] In some embodiments, a PD-1 axis binding antagonist comprises an anti-PD-1 antibody, an anti PD-L1 antibody, and / or an anti-PD-L2 antibody, or a nucleic acid, such as RNA, encoding the binding antagonist. In some embodiments, the encoding RNA is targeted to the liver for systemic availability. Liver cells can be efficiently transfected and are able to produce large amounts of protein.

[0020] In some embodiments, the EpCAM is human EpCAM. In some embodiments, the CD137 is human CD137. In some embodiments, human EpCAM comprises the sequence set forth in SEQ ID NO: 59. In some embodiments, human CD137 comprises the sequence set forth in SEQ ID NO: 62.

[0021] In some embodiments, said first antigen-binding region binding to EpCAM binds to EpCAM expressed on tumor cells. In some embodiments, the PD-1 axis binding antagonist can comprise a PD-1 binding antagonist. In some embodiments, the PD-1 binding antagonist can comprise an anti-PD-1 antibody. In some embodiments, the PD-1 antibody can be selected from the group consisting of IgGl-PDl, nivolumab, cemiplimab, dostarlimab or pembrolizumab. In some embodiments, the PD-1 antibody can be IgGl-PDl.

[0022] In some embodiments, the PD-1 axis binding antagonist can comprise a PD-L1 binding antagonist. In some embodiments, the PD-L1 binding antagonist can comprise an anti-PD-Ll antibody. In some embodiments, the anti- PD-L1 antibody can be selected from the group consisting of atezolizumab, avelumab or durvalumab.

[0023] In some embodiments, the PD-1 axis binding antagonist can comprise a PD-L2 binding antagonist. In some embodiments, the PD-L2 binding antagonist can comprise an anti-PD-L2 antibody. In some embodiments, the anti- PD-L2 antibody can be selected from the group consisting of OT17B10 and PDL2 / 1850.

[0024] In some embodiments, the disease or disorder concerns unregulated cell growth, such as a cancer or a tumor or a solid tumor.

[0025] In some embodiments, the cancer, tumor, or solid tumor can be selected from the group consisting of cholangiocarcinoma (bile duct cancer), gastric / gastroesophageal junction (GEJ) cancer, melanoma, ovarian cancer, lung cancer (e.g., non-small cell lung cancer (NSCLC)), colorectal cancer, head and neck cancer, gastric cancer, breast cancer, renal cancer, urothelial cancer, bladder cancer, esophageal cancer, pancreatic cancer, hepatic cancer, thymoma and thymic carcinoma, brain cancer, glioma, adrenocortical carcinoma, thyroid cancer, other skin cancers, sarcoma, multiple myeloma, leukemia, lymphoma, myelodysplastic syndromes, endometrial cancer, prostate cancer, penile cancer, cervical cancer, Hodgkin's lymphoma, non-Hodgkin's lymphoma, Merkel cell carcinoma and mesothelioma. In some embodiments, the disease or disorder can be cholangiocarcinoma (bile duct cancer) or gastric / gastroesophageal junction (GEJ).

[0026] In some embodiments, the subject can be a mammalian subject, such as a human subject.

[0027] In some embodiments, the subject has not received prior treatment for the disease or disorder. In some embodiments, the subject has received prior treatment for the disease or disorder. In some embodiments, the subject has not received prior treatment with a checkpoint inhibitor, such as CTLA-4 treatment.

[0028] In some embodiments, the method can further comprise administering to the subject one or more additional therapeutic agents. Such one or more additional therapeutic agents can comprise one or more chemotherapeutic agents, such as platinum-based compounds (e.g., cisplatin, oxaliplatin, and carboplatin), taxane-based compounds (e.g., paclitaxel and nab-paclitaxel), nucleoside analogs (e.g., 5-fluorouracil and gemcitabine), and combinations thereof (e.g., cisplatin / carboplatin + 5-fluorouracil or nab-paclitaxel + gemcitabine).

[0029] In some embodiments, administering the binding agent to the subject comprises administering a nucleic acid encoding the binding agent. In some embodiments, administering the PD(L)-1 axis binding antagonist to the subject comprises administering a nucleic acid encoding the PD(L)-1 axis binding antagonist. In some embodiments, administering the binding agent and the PD(L)-1 binding antagonist to the subject comprises administering a nucleic acid encoding both the binding agent and the PD(L)-1 binding antagonist. In some embodiments, the nucleic acid is RNA. In an aspect, the present disclosure provides a polynucleotide or a set of polynucleotides encoding the binding agent and / or the PD-1 axis binding antagonist defined above.

[0030] In an aspect, the present disclosure provides a kit comprising, in one or more vials, (i) a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137, or nucleic acid encoding the binding agent, (ii) a PD-1 axis binding antagonist or a nucleic acid encoding the PD-1 binding antagonist, and optionally (iii) one or more therapeutic agents. In some embodiments, the kit further comprises instructions for the use of the kit in treating or preventing a disease or disorder in a subject. In some embodiments of the kit, the binding agent or encoding nucleic acid and the PD-1 binding antagonist or encoding nucleic acid are in separate vials.

[0031] In an aspect, the present disclosure provides a kit of the disclosure for use in a method of treating or preventing a disease or disorder in a subject, preferably wherein the disease or disorder is a tumor, solid tumor, or cancer, wherein said method comprises (i) administering to the subject the binding agent, and (ii) administering to the subject the PD-1 binding antagonist, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD-1 binding antagonist.

[0032] In an aspect, the present disclosure provides a kit of the disclosure for use in a method of reducing or preventing progression of a tumor, solid tumor or cancer in a subject, wherein said method comprises (i) administering to the subject the binding agent, and (ii) administering to the subject the PD-1 binding antagonist, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD-1 binding antagonist.

[0033] The various embodiments relating to the method of treatment aspect of the disclosure are equally applicable to the aspect pertaining to the kit of the disclosure and it use.

[0034] An aspect of the present disclosure provides a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137 for use in a method for treating or preventing a disease or disorder in a subject, said method comprising administering the binding agent to the subject in combination with a PD-1 axis binding antagonist, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD-1 axis binding antagonist.

[0035] An aspect of the present disclosure provides a PD-1 axis binding antagonist for use in a method for treating or preventing a disease or disorder in a subject, said method comprising administering the PD-1 axis binding antagonist to the subject in combination with a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137, wherein the administration of the PD-1 axis binding antagonist is prior to, simultaneously with, or after administration of the binding agent.

[0036] An aspect of the present disclosure provides a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137 and a PD-1 axis binding antagonist for use in a method for treating or preventing a disease or disorder in a subject, said method comprising administering the binding agent and the PD-1 axis binding antagonist to the subject, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD-1 axis binding antagonist. In some embodiments, the method for treating or preventing a disease or disorder in a subject is a method for reducing or preventing progression of a tumor or treating cancer in a subject.

[0037] An aspect of the present disclosure provides for use of a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137 for the manufacture of a medicament for treating or preventing a disease or disorder in a subject in combination with a PD-1 axis binding antagonist.

[0038] An aspect of the present disclosure provides for use of a PD-1 axis binding antagonist for the manufacture of a medicament for treating or preventing a disease or disorder in a subject, in combination with a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137.

[0039] An aspect of the present disclosure provides for use of (i) a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137 and (ii) a PD-1 axis binding antagonist for the manufacture of a medicament for treating or preventing a disease or disorder in a subject.

[0040] In some embodiments, the disease or disorder a tumor, solid tumor or cancer.

[0041] The embodiments relating to the method of treatment aspect are equally applicable to the aspects concerning the use of the binding agent and PD-1 axis binding antagonist.

[0042] An aspect of the present disclosure provides for a medical preparation comprising the binding agent and the PD-1 axis binding antagonist as defined in the present disclosure. In an embodiment, the medical preparation is for use in any of the methods of treatment disclosed in the present disclosure.

[0043] Brief description of the Figures

[0044] Figure 1: Relative EpCAM expression on tumor cells

[0045] Fig 1 shows the relative EpCAM expression on T84, DiFi, HPAF-II, NCI-N87, Calu-3, NCI-H747, and A549 tumor cell lines as determined by flow cytometry. Data shown is A geometric mean fluorescence intensity (gMFI) for each tumor cell line calculated as: Geomean fluorescence intensity (APC) of the EpCAM antibody - Geomean fluorescence (APC) of the non-binding control antibody.

[0046] Figure 2: Binding of bivalent EpCAM antibodies and their monovalent counterparts to DiFi, HPAF-II or A549 cells.

[0047] Mono- and bivalent binding of EpCAM antibodies to DiFi, HPAF-II or A549 cell lines was determined by flow cytometry. IgGl-bl2-FEAL was included as negative control in all experiments (open squares). Data shown is mean fluorescence intensity (MFI; R-Phycoerythrin [PE]) ± standard deviation (SD) of duplicate measurements of one representative experiment. A. Binding of bsIgGl-bl2-FEALxEpCAM-A37-FEAR (closed circles) and IgGl-EpCAM- A37-FEAR (open triangles) antibodies to DiFi, HPAF-II or A549 cells. B. Binding of bsIgGl-bl2-FEALxEpCAM-C52- FEAR (closed circles) and IgGl-EpCAM-C52-FEAR (open triangles) antibodies to DiFi, HPAF-II or A549 cells. C. Binding of bsIgGl-bl2-FEALxEpCAM-UBS-54-FEAR (closed circles) and IgGl-EpCAM-UBS-54-FEAR (open triangles) antibodies to DiFi, HPAF-II or A549 cells. D. Binding of IgGl-EpCAM-323-A3-FEAR (open triangles) antibodies to DiFi or HPAF-II cells. E. Binding of bsIgGl-CD137-009-HC7LC2-FEAL / EpCAM-A37-FEAR and bsIgGl-EpCAM-A37- FERL / CD137-009-HC7LC2-FEAR and non-binding control bispecific antibodies bsIgGl-bl2-FERL / CD137-HC7LC2- FEAR and bsIgGl-bl2-FERL / bl2-FEAR to DiFi cells. Figure 3: Binding of EpCAM antibodies to full length human, cynomolgus monkey or mouse EpCAM transfected into CHO-S cells.

[0048] Binding of monovalent and bivalent EpCAM antibodies was analysed using CHO-S cells transiently transfected with full length human, cynomolgus monkey or mouse EpCAM. As negative control, binding to non-transfected CHO-WT cells was evaluated. Data are presented as geomean fluorescence intensity (gMFI) R-PE values of two technical replicates ± SD. A. Binding of IgGl-EpCAM-UBS-54-FEAR, IgGl-EpCAM-A37-FEAR, IgGl-EpCAM-C52-FEAR and IgGl-EpCAM-343-A3. B. Binding of bs!gGl-bl2-FEAL / EpCAM-UBS-54-FEAR, bs!gGl-EpCAM-A37-FERL / bl2-FEAR, bs!gGl-bl2-FEAL / EpCAM-C52-FEAR, and bs!gGl-bl2-FEAL / EpCAM-323-A3-FEAR.

[0049] Figure 4: Induction of 4-lBB-dependent luciferase activity by an EpCAMx4-lBB bispecific antibody in a cell-based reporter assay.

[0050] HEK293_NKF_h4-lBB_gfpJuc reporter cells were either co-cultured with EpCAM-expressing OV-90-SC12 cells, or cultured alone (medium), in the presence of serial dilutions of BsIgGl-CD137-005-FEAR / EpCAM-323-A3-FEAL overnight. Luciferase activity was quantified by luminescence measurements. Fold-induction of luciferase activity relative to cultures without antibody (dotted line) is shown. Error bars depict SD of duplicate wells. Data from one representative experiment out of three are shown.

[0051] Figure 5: Enhancement of PBMC proliferation in vitro by EpCAMx4-lBB bispecific antibodies in PBMC-DiFi cell cocultures.

[0052] EpCAMx4-lBB bispecific antibodies were tested in an in vitro PBMC proliferation assay using EpCAM-expressing DiFi cells in co-culture with CFSE-labeled PBMCs. Cells were cultured in the presence of anti-CD3 (0.1μg / mL ) with or without the addition of EpCAMx4-lBB or EpCAMxbl2 bispecific antibodies or non-binding control IgGl-bl2-FEAL at the indicated concentrations for 96 h. The number of CFSE-positive cells was extracted and used to calculate the division index. Data shown are the mean division index ± SD of duplicate measurements obtained from one representative experiment. A. Division index of PBMC proliferation induced by bsIgGl-bl2-FEAL / EpCAM-UBS54- FEAR (closed triangles) and bs!gGl-CD137-009-HC7LC2-FEAL / EpCAM-UBS54-FEAR (closed squares) antibodies. B. Division index of PBMC proliferation induced by bs!gGl-bl2-FEAL / EpCAM-A37-FEAR (closed triangles) and bsIgGl- CD137-009-HC7LC2-FEAL / EpCAM-A37-FEAR (closed squares) antibodies. C. Division index of PBMC proliferation induced by bs!gGl-bl2-FEAL / EpCAM-C52-FEAR (closed triangles) and bs!gGl-CD137-009-HC7LC2-FEAL / EpCAM- C52-FEAR (closed squares) antibodies. D. Division index of PBMC proliferation induced by bs!gGl-bl2-FEAL / EpCAM- 323-A3-FEAR (closed triangles) and bs!gGl-CD137-009-HC7LC2-FEAUEpCAM-323-A3-FEAR (closed squares) antibodies.

[0053] Figure 6: Enhancement of CD4+ and CD8+ T-cell proliferation in vitro by EpCAMx4-lBB bispecific antibodies in PBMC-DiFi cell co-cultures.

[0054] EpCAMx4-lBB bispecific antibodies were tested in an in vitro T-cell proliferation assay using EpCAM-expressing DiFi cells in co-culture with CFSE-labeled PBMCs. Cells were cultured in the presence of anti-CD3 (0.1μg / mL ) with or without the addition of EpCAMx4-lBB (grey bars) or EpCAMxbl2 (striped bars) bispecific antibodies (10μg / mL ) or non-binding control IgGl-bl2-FEAL (10μg / mL ; open bars) for 96 h. The number of CFSE-positive cells was evaluated as a measure of absolute CD4+ (A) or CD8+ (B) T-cell counts by flow cytometry and the division index were calculated. Data shown are the mean division index ± SD of duplicate measurements obtained from one representative experiment. Figure 7: Enhancement of PBMC proliferation in vitro by EpCAMx4-lBB bispecific antibodies in PBMC-tumor cell cocultures using various tumor cell lines.

[0055] EpCAMx4-lBB bispecific antibodies were tested in an in vitro PBMC proliferation assay using EpCAM-expressing T84, DiFi, HPAF-II, NCI-N87, Calu-3 or NCI-H747 tumor cells in co-culture with CFSE-labeled PBMCs. Cells were cultured in the presence of anti-CD3 (0.1μg / mL ) and EpCAMx4-lBB or EpCAMxbl2 bispecific antibodies or non- binding control IgGl-bl2-FEAL at the indicated concentrations for 96 h. The number of CFSE-positive cells was evaluated as a measure of absolute PBMC counts by flow cytometry and the division index was calculated. Data shown are the mean division index ± SD of duplicate measurements obtained from one representative experiment using (A) PBMC-T84 co-cultures, (B) PBMC-DiFi co-cultures, (C) PBMC-HPAF-II co-cultures, (D) PBMC-NCI-N87 co- cultures, (E) PBMC-Calu-3 co-cultures or (F) PBMC-NCI-H747 co-cultures.

[0056] Figure 8. Enhancement of human CD4+ and CD8+ T-cell in vitro proliferation in DiFi tumor cell co-cultures by EpCAMx4-lBB bispecific antibodies with Fc-domains containing inertness mutations.

[0057] EpCAMx4-lBB bispecific antibodies were tested in an in vitro PBMC proliferation assay using EpCAM-expressing DiFi tumor cells in co-culture with CFSE-labeled human PBMCs. Cells were cultured in the presence of anti-CD3 (0.1 pg / mL) and EpCAM-FERLx4-lBB-FEAR (closed diamonds), EpCAM-FEARx4-lBB-FEAL (closed squares), bl2- FERLx4-lBB-FEAR (left-half-closed triangles), EpCAM-FERLxbl2-FEAR (right-half-closed triangles) bispecific antibodies or non-binding control bl2-FERLxbl2-FEAR (open triangles) at the indicated concentrations for 96 h. The number of CFSE-positive (A) CD4+ and (B) CD8+ T cells was evaluated as a measure of absolute T-cell counts by flow cytometry and the division index was calculated. Data shown are non-linear 4-parameter variable slope fits of the mean division index ± SD of duplicate measurements obtained from one representative experiment using PBMC-DiFi co-cultures.

[0058] Figure 9: Enhancement of tumor-infiltrating lymphocyte expansion by EpCAMx4-lBB bispecific antibodies in patient- derived tumor specimens ex vivo.

[0059] Tumor tissues resected from non-small cell lung cancer patients were cut into pieces of 1-2 mm3and cultured in the presence of IL-2 (50 U / mL) and 0.2-5μg / mL BsIgGl-CD137-009-HC7LC2-FEAl / EpCAM-A37-FEAR or BsIgGl- CD137-009-HC7LC2-FEAL / EpCAM-323-A3-FEAR, or with IL-2 only (w / o Ab) for 14 days. IL-2 concentrations were reduced stepwise on days 7 and 10, to 33 U / mL and 15 U / mL, respectively. Absolute cell numbers after culture were determined by flow cytometry. Total TIL, NK cell, CD8+, and CD4+ T cell numbers are shown for cultures derived from one exemplary patient, out of two patients analyzed. Data from 3-4 individual replicates and average of replicates is shown. Error bars depict SD.

[0060] Figure 10: Antitumor activity of an EpCAMx4-lBB bispecific antibody in hEpCAM mice bearing hEpCAM- overexpressing MC38 tumors.

[0061] Mice transgenic for human EpCAM protein (hEpCAM mice) were inoculated with 5x l05MC38 tumor cells overexpressing human EpCAM protein (MC38_hEpCAM). Treatment was commenced when tumors reached an average size of approximately 30 mm3. Mice were treated intraperitoneally with 100 pg BsIgG2amm-EpCAM-323- A3-AALT / m4-lBB-3H3-AAKR or the negative control antibody IgG2amm-bl2-AAKR on days 12, 17, 21, 24, 28, and 31 after tumor inoculation (vertical dotted lines). (A) Average tumor volumes per group are shown, error bars depict SEM. Growth curves contain last measured tumor volume of sacrificed mice (last observation carried forward). **, P<0.01; Two-Way repeated-measures ANOVA. (B) Per cent survival. *, P<0.05; Log-rank (Mantel- Cox) test. Figure 11. Enhancement of human CD4+ and CD8+ T-ceii proliferation in PBMC-DiFi tumor cell co-cuitures by EpCAMx4-lBB bispecific antibodies in comparison to the combination of bivalent EpCAM- and 4-lBB-specific monoclonal antibodies.

[0062] Bispecific antibody bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR was compared to bivalent IgGl-EpCAM- A37-FERL and IgGl-CD137-009-HC7LC2-FEAR (either alone or in combination) in an in vitro PBMC proliferation assay using EpCAM-expressing DiFi tumor cells in co-culture with CellTrace Violet (CTV)-labeled human PBMCs. Cells were cultured in the presence of anti-CD3 (0.1μg / mL ; dotted line) and EpCAMx4-lBB, IgGl-EpCAM-A37-FERL and / or IgGl-CD137-009-HC7LC2-FEAR at the indicated concentrations for 96 h. CD4+ and CD8+ T proliferation was evaluated by flow cytometry analysis of CTV label dilution. Data shown are mean expansion index ± SD of duplicate measurements obtained from one representative experiment.

[0063] Figure 12. Enhancement of human CD4+ and CD8+ T-cell proliferation in PBMC-DiFi tumor cell co-cultures by EpCAMx4-lBB bispecific antibodies in comparison to the combination of bs!gGl-EpCAM-A37-FERL / bl2-FEAR and bs!gGl-bl2-FERL / CD137-HC7LC2-FEAR.

[0064] Bispecific antibody bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR was compared to the combination of bsIgGl-EpCAM-A37-FERL / bl2-FEAR and bs!gGl-bl2-FERL / CD137-HC7LC2-FEAR in an in vitro PBMC proliferation assay using EpCAM-expressing DiFi tumor cells in co-culture with CTV-labeled human PBMCs. Cells were cultured in the presence of anti-CD3 (0.1μg / mL ; dotted line) and bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR or with the combination of bs!gGl-EpCAM-A37-FERL / bl2-FEAR and bs!gGl-bl2-FERL / CD137-HC7LC2-FEAR at the indicated concentrations for 96 h. CD4+ and CD8+ T proliferation was evaluated by flow cytometry analysis of CTV label dilution. Data shown are mean percentage divided cells ± SD of duplicate measurements obtained from one representative experiment.

[0065] Figure 13. Enhancement of human CD4+ and CD8+ T-cell activation in PBMC-DiFi tumor cell co-cultures by EpCAMx4-lBB bispecific antibodies in comparison to the combination of bs!gGl-EpCAM-A37-FERL / bl2-FEAR and bs!gGl-bl2-FERL / CD137-HC7LC2-FEAR.

[0066] EpCAMx4-lBB bispecific antibody bs!gGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR was compared to the combination of bs!gGl-EpCAM-A37-FERL / bl2-FEAR and bsIgGl-bl2-FERUCD137-HC7LC2-FEAR in an in vitro PBMC proliferation assay using EpCAM-expressing DiFi tumor cells in co-culture with human PBMCs. Cells were cultured in the presence of anti-CD3 (0.1μg / mL ; dotted line) and bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2- FEAR or with the combination of bs!gGl-EpCAM-A37-FERL / bl2-FEAR and bsIgGl-bl2-FERL / CD137-HC7LC2-FEAR at the indicated concentrations for 96 h. The percentage of CD4+ and CD8+ T cells expressing CD25 (A) or 4-1BB (B), as well as the geometric mean fluorescence intensity (FI) in the CD25+ or 4-1BB+ population was determined by flow cytometry. Data shown are mean ± SD of duplicate measurements obtained from one representative experiment.

[0067] Figure 14. Enhancement of human CD4+ and CD8+ T-ceii proliferation in cancer patient-derived PBMC - DiFi tumor cell co-cultures by EpCAMx4-lBB bispecific antibodies in comparison to the combination of bs!gGl-EpCAM-A37- FERL / bl2-FEAR and bs!gGl-bl2-FERL / CD137-009-HC7LC2-FEAR.

[0068] EpCAMx4-lBB bispecific antibody bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR was compared to the combination of bsIgGl-EpCAM-A37-FERL / bl2-FEAR and bsIgGl-bl2-FERL / CD137-009-HC7LC2-FEAR in an in vitro proliferation assay using EpCAM-expressing DiFi tumor cells in co-culture with CTV-labeled cancer patient-derived PBMC. Cells were cultured in the presence of anti-CD3 (0.1μg / mL ; dotted line) and bsIgGl-EpCAM-A37- FERL / CD137-009-HC7LC2-FEAR or with the combination of bsIgGl-EpCAM-A37-FERL / bl2-FEAR and bsIgGl-bl2- FERL / CD137-009-HC7LC2-FEAR at the indicated concentrations for 96 h. CD4+ and CD8+ T cell proliferation was evaluated by flow cytometry analysis of CTV label dilution. Data shown are mean percentage divided cells ± SD of duplicate measurements obtained from one experiment.

[0069] Figure 15. Enhancement of human CD4+ and CD8+ T-cell activation in cancer patient-derived PBMC - DiFi tumor cell co-cultures by EpCAMx4-lBB bispecific antibodies in comparison to the combination of bs!gGl-EpCAM-A37- FERL / bl2-FEAR and bsIgGl-bl2-FERL / CD137-009-HC7LC2-FEAR.

[0070] EpCAMx4-lBB bispecific antibody bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR was compared to the combination of bsIgGl-EpCAM-A37-FERL / bl2-FEAR and bsIgGl-bl2-FERUCD137-009-HC7LC2-FEAR in an in vitro proliferation assay using EpCAM-expressing DiFi tumor cells in co-culture with cancer patient-derived PBMCs. Cells were cultured in the presence of anti-CD3 (0.1μg / mL ; dotted line) and bsIgGl-EpCAM-A37-FERL / CD137-009- HC7LC2-FEAR or with the combination of bsIgGl-EpCAM-A37-FERL / bl2-FEAR and bsIgGl-bl2-FERL / CD137-009- HC7LC2-FEAR at the indicated concentrations for 96 h. The percentage of CD4+ and CD8+ T cells expressing CD25 (A) and 4-1BB (B), as well as the geometric mean fluorescence intensity (FI) in the CD25+ or 4-1BB+ population was determined by flow cytometry. Data shown are mean ± SD of duplicate measurements obtained from one experiment.

[0071] Figure 16. An EpCAMx4-lBB bispecific antibody enhances CD107a and GzmB expression by CD8*~ T cells PBMC-derived CD8+T cells expressing a CLDN6-specific TCR were co-cultured with MDA-MB-231_hCLDN6_hEpCAM tumor cells for 2 d in the presence of bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR or control antibodies. CD107a and GzmB expression by CD8+T cells were analyzed by flow cytometry. (A) Representative flow cytometry plots. (B-C) Dose-response curves of CD107a (C) and GzmB (D) expression levels. Data shown are average and SD of duplicate measurements from one of six donors tested. (D) Normalized CD107a and (E) GzmB expression levels (expression levels of bsIgGl-bl2-FERL / bl2-FEAR were set to 1 for each donor). Pooled data from six donors assessed in two independent experiments are shown. Error bars indicate SD. ****, P<0.0001; ***, P<0.001; **, PcO.Ol; *, P<0.05; Friedman test with Dunn's multiple comparisons test. GMFI = geometric mean fluorescence intensity; GzmB = granzyme B; PBMC = peripheral blood mononuclear cell; SD = standard deviation.

[0072] Figure 17. An EpCAMx4-lBB bispecific antibody enhances CD8* T-cell mediated cytotoxicity towards tumor cells PBMC-derived CD8+T cells expressing a CLDN6-specific TCR were co-cultured with MDA-MB-231_hCLDN6_hEpCAM tumor cells for 5 to 6 d in the presence of bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR or control antibodies. Cell index values were derived from impedance measurements conducted at 2 to 3 h intervals. (A) Cell index curves in co-cultures from one representative donor are shown. Symbols represent average cell index values of duplicate wells. Data from every second impedance measurement (Ze., every 4 to 6 h) are shown for better visibility. (B) AUC analysis was performed using cell index data over the entire duration of the co-cultures. AUC of each treatment condition was normalized to bsIgGl-ctrl-bl2-FERL / bl2-FEAR-treated cultures from the same donor. Pooled data from all six donors assessed in two independent experiments are shown. Error bars indicate SD. ***, PcO.OOl; **, P<0.01; *, P<0.05; Friedman test with Dunn's multiple comparisons test.

[0073] AUC = area under the curve; PBMC = peripheral blood mononuclear cell; SD = standard deviation.

[0074] Figure 18. Binding of EpCAMx4-lBB bispecific antibodies with different Fc-inertness mutations (FER / FEA,

[0075] FEA / FEA, FER / FER) to FcyRs

[0076] The binding of EpCAMx4-lBB bispecific antibodies with various combinations of Fc-inertness mutations (FER / FEA, FEA / FEA, FER / FER), and the binding of monoclonal antibodies IgGl-EpCAM-A37-FERL and IgGl-CD137-009- HC7LC2-FEAR to immobilized human recombinant FcyR proteins was analyzed by SPR: FcyRIa, FcyRIIa-H131, FcyRIIa-R131, FcyRIIb, FcyRIIIa-F158, and FcyRIIIa-V158. The antibody IgGl-bl2 (wild-type Fc) was included as a positive control for FcyR binding. The relative binding response in RU (response units) is measured by setting the sensorgram at 0 RU at the moment of SPR analyte injection. Each sample that was analyzed on an active surface was also analyzed on a parallel reference surface used for background correction. Shown are data of single measurements of one experiment.

[0077] Figure 19. IFNy secretion induced by the combination of bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR and pembroiizumab in PBMC-EpCAM+ tumor cell cocultures

[0078] Healthy donor PBMCs were cocultured with EpCAM-expressing (EpCAM+) DiFi or CAL27 tumor cells (at a ratio of 16:1) in the presence of an anti-CD3 antibody (0.1μg / mL ) and treated with bsIgGl-EpCAM-A37-FERL / CD137-009- HC7LC2-FEAR, 1 μg / mL pembroiizumab (either alone or in combination) or control antibodies (bsIgGl-EpCAM-A37- FERL / bl2-FEAR and bs!gGl-bl2-FERL / CD137-009-HC7LC2-FEAR [3μg / mL of each antibody] or IgG4 [1μg / mL ]) for 96 h in order to determine IFNy secretion. IFNy concentration in the supernatant was analyzed by ELISA. Data shown are single IFNy measurements from a representative donor (n=5 [DiFi] or n=4 [CAL27]). Dotted black line represents anti-CD3-stimulated cocultures in the absence of treatment antibody.

[0079] Figure 20. IFNy secretion induced by the combination of bs!gGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR and IgGl-PDl in PBMC-EpCAM+ tumor cell cocultures

[0080] Healthy donor PBMCs were cocultured with EpCAM-expressing (EpCAM+) DiFi or CAL27 tumor cells (at a ratio of 16:1) in the presence of an anti-CD3 antibody (0.1μg / mL ) and treated with bsIgGl-EpCAM-A37-FERL / CD137-009- HC7LC2-FEAR, 1 μg / mL IgGl-PDl (either alone or in combination) or control antibodies (bsIgGl-EpCAM-A37- FERL / bl2-FEAR and bsIgGl-bl2-FERL / CD137-009-HC7LC2-FEAR [3μg / mL of each antibody] or IgGl-bl2-FERR [1 pg / mL]) for 96 h in order to determine IFNy secretion. IFNy concentration in the supernatant was analyzed by ELISA. Data shown are single IFNy measurements from a representative donor (n=5 [DiFi] or n=4 [CAL27]). Dotted black line represents anti-CD3-stimulated cocultures in the absence of treatment antibody.

[0081] Figure 21. Combining an EpCAMxd-IBB bispecific antibody with an anti-PD-1 antibody potentiates single-agent activities leading to enhanced CDEF T cell-expansion in ex vivo human CRC tissue cultures

[0082] Fragments of tumor tissue resected from CRC patients were cultured in the presence of IL-2 (<50 U / mL) for 2 weeks and treated with bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR (0.2 μg / mL), pembroiizumab (0.8 pg / mL), IgGl-PDl (0.8 μg / mL ), or the indicated combinations on days 0 and 3. CD8+ T-cell numbers were determined by flow cytometry at the end of the culture. Data shown are 3-4 replicates and median of these replicates from one patient out of three patients tested. The dotted line represents the median CD8+ T-cell number of cultures without antibody treatment.

[0083] Figure 22. Combining an EpCAMx4-lBB bispecific antibody with an anti-PD-(L)l antibody potentiates single-agent activities leading to enhanced CD107a and GzmB expression by CDtF T cells

[0084] PBMC-derived CD8+T cells expressing a CLDN6-specific TCR were co-cultured with MDA-MB-231_hCLDN6_hEpCAM tumor cells for 2d in the presence of bsIgGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR, anti-PD-(L)l antibodies, a combination of both, or control antibodies. The percentage of CD107a and GzmB-expressing CD8+T cells was determined by flow cytometry analysis. Data shown are average and standard deviation of duplicate wells from representative donors (of 4 to 11 donors tested). (A) The combination with nivolumab, pembroiizumab, IgGl-PDl, and atezolizumab and (B) the combination with cemipiimab and dostarlimab were tested in independent experiments using PBMCs of different donors. Anti-PD-(L)! antibodies were used at concentrations sufficient to achieve maximal single-agent activity in antigen-specific proliferation assays of human CD8+T cells, as determined previously.

[0085] Figure 23. Combining an EpCAMx4-lBB bispecific antibody with an anti-PD-(L)! antibody potentiates single-agent activities leading to enhanced CD8* T cell-mediated tumor-cell killing

[0086] PBMC-derived CD8+T cells expressing a CLDN6-specific TCR were co-cultured with MDA-MB-231_hCLDN6_hEpCAM tumor cells for 5 to 6 d in the presence of bs!gGl-EpCAM-A37-FERL / CD137-009-HC7LC2-FEAR, anti-PD-(L)! antibodies, a combination of both, or control antibodies. Tumor-cell killing was assessed by impedance measurements of the adherent tumor-cell layer conducted at 2- to 3-h intervals. (A) Curves of cell indices normalized to the time point of co-culture initiation (set to 1) in co-cultures from one representative donor and pembrolizumab as an exemplary anti-PD-(L)l antibody are shown. Symbols represent average cell index values of duplicate wells. Data from every second impedance measurement ( / >., every 4 to 6 h) are shown for better visibility. (B, C) AUC analyses were performed using normalized cell index data over the entire duration of the co- cultures. AUC of each treatment condition was normalized to bs!gGl-bl2-FERL / bl2-FEAR-treated cultures from the same donor. The combination with nivolumab, pembrolizumab, IgGl-PDl, and atezolizumab (B) and the combination with cemiplimab and dostarlimab (C) were tested in independent experiments using PBMCs of different donors. Data shown are AUC of representative donors (of 4 to 11 donors tested). Anti-PD-(L)1 antibodies were used at concentrations sufficient to achieve maximal single-agent activity in antigen-specific proliferation assays of human CD8+T cells, as determined previously.

[0087] Description of the Sequences

[0088] Table 1 - Sequences

[0089] Detailed Description of the Disclosure

[0090] Although the present disclosure is further described in more detail below, it is to be understood that this disclosure is not limited to the particular methodologies, protocols and reagents described herein as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present disclosure which will be limited only by the appended claims. Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art.

[0091] In the following, the elements of the present disclosure will be described in more detail. These elements are listed with specific embodiments; however, it should be understood that they may be combined in any manner and in any number to create additional embodiments. The variously described examples and preferred embodiments should not be construed to limit the present disclosure to only the explicitly described embodiments. This description should be understood to support and encompass embodiments which combine the explicitly described embodiments with any number of the disclosed and / or preferred elements. Furthermore, any permutations and combinations of all described elements in this application should be considered disclosed by the description of the present application unless the context indicates otherwise.

[0092] Preferably, the terms used herein are defined as described in "A multilingual glossary of biotechnological terms: (IUPAC Recommendations)", H.G.W. Leuenberger, B. Nagel, and H. Kolbl, Eds., Helvetica Chimica Acta, CH-4010 Basel, Switzerland, (1995).

[0093] The practice of the present disclosure will employ, unless otherwise indicated, conventional chemistry, biochemistry, cell biology, immunology, and recombinant DNA techniques which are explained in the literature in the field (cf., e.g., Organikum, Deutscher Verlag der Wissenschaften, Berlin 1990; Streitwieser / Heathcook, "Organische Chemie", VCH, 1990; Beyer / Walter, "Lehrbuch der Organischen Chemie", S. Hirzel Verlag Stuttgart, 1988; Carey / Sundberg, "Organische Chemie", VCH, 1995; March, "Advanced Organic Chemistry", John Wiley & Sons, 1985; Rbmpp Chemie Lexikon, Falbe / Regitz (Hrsg.), Georg Thieme Verlag Stuttgart, New York, 1989; Molecular Cloning: A Laboratory Manual, 2nd Edition, J. Sambrook et al. eds., Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989.

[0094] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by the context. The use of any and all examples, or exemplary language {e.g., "such as"), provided herein is intended merely to better illustrate the present disclosure and does not pose a limitation on the scope of the present disclosure otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the present disclosure.

[0095] Recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein.

[0096] Several documents are cited throughout the text of this specification. Each of the documents cited herein (including all patents, patent applications, scientific publications, manufacturer's specifications, instructions, etc.), whether supra or infra, are hereby incorporated by reference in their entirety. Nothing herein is to be construed as an admission that the invention is not entitled to antedate such disclosure by virtue of prior invention. Definitions

[0097] In the following, definitions will be provided which apply to all aspects of the present disclosure. The following terms have the following meanings unless otherwise indicated. Any undefined terms have their art recognized meanings.

[0098] Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated member, integer or step or group of members, integers or steps but not the exclusion of any other member, integer or step or group of members, integers or steps. The term "consisting essentially of means excluding other members, integers or steps of any essential significance. The term "comprising" encompasses the term "consisting essentially of which, in turn, encompasses the term "consisting of. Thus, at each occurrence in the present application, the term "comprising" may be replaced with the term "consisting essentially of or "consisting of. Likewise, at each occurrence in the present application, the term "consisting essentially of may be replaced with the term "consisting of.

[0099] The terms "a", "an" and "the" and similar references used in the context of describing the present disclosure (especially in the context of the claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by the context.

[0100] Where used herein, "and / or" is to be taken as specific disclosure of each of the two specified features or components with or without the other. For example, "X and / or Y" is to be taken as specific disclosure of each of (i) X, (ii) Y, and (iii) X and Y, just as if each is set out individually herein.

[0101] In the context of the present disclosure, the term "about" denotes an interval of accuracy that the person of ordinary skill will understand to still ensure the technical effect of the feature in question. The term typically indicates deviation from the indicated numerical value by ±5%, ±4%, ±3%, ±2%, ±1%, ±0.9%, ±0.8%, ±0.7%, ±0.6%, ±0.5%, ±0.4%, ±0.3%, ±0.2%, ±0.1%, ±0.05%, and for example ±0.01%. As will be appreciated by the person of ordinary skill, the specific such deviation for a numerical value for a given technical effect will depend on the nature of the technical effect. For example, a natural or biological technical effect may generally have a larger such deviation than one for a man-made or engineering technical effect.

[0102] The term "binding agent" in the context of the present disclosure refers to any agent capable of binding to desired antigens. In certain embodiments of the present disclosure, the binding agent is an antibody, antibody fragment, or construct thereof. The binding agent may also comprise synthetic, modified or non-naturally occurring moieties, in particular non-peptide moieties. Such moieties may, for example, link desired antigen-binding functionalities or regions such as antibodies or antibody fragments. In one embodiment, the binding agent is a synthetic construct comprising antigen-binding CDRs or variable regions.

[0103] The term "immunoglobulin" relates to proteins of the immunoglobulin superfamily, preferably to antigen receptors such as antibodies or the B cell receptor (BCR). The immunoglobulins are characterized by a structural domain, i.e., the immunoglobulin domain, having a characteristic immunoglobulin (Ig) fold. The term encompasses membrane bound immunoglobulins as well as soluble immunoglobulins. Membrane bound immunoglobulins are also termed surface immunoglobulins or membrane immunoglobulins, which are generally part of the BCR. Soluble immunoglobulins are generally termed antibodies. The structure of immunoglobulins has been well characterized. See, e.g., Fundamental Immunology Ch. 7 (Paul, W., ed., 2nded. Raven Press, N.Y. (1989)). Briefly, immunoglobulins generally comprise several chains, typically two identical heavy chains and two identical light chains which are linked via disulfide bonds. These chains are primarily composed of immunoglobulin domains or regions, such as the VL or VL (variable light chain) domain / region, CLor CL (constant light chain) domain / region, VHor VH (variable heavy chain) domain / region, and the CH or CH (constant heavy chain) domains / regions 04 (CHI), CH2 (CH2), CH3 (CH3), and CH4 (CH4). The heavy chain constant region typically is comprised of three domains, CHI, CH2, and CH3. The hinge region is the region between the CHI and CH2 domains of the heavy chain and is highly flexible. Disulfide bonds in the hinge region are part of the interactions between two heavy chains in an IgG molecule. Each light chain typically is comprised of a VL and a CL. The light chain constant region typically is comprised of one domain, CL. The VH and VL regions may be further subdivided into regions of hypervariability (or hypervariable regions which may be hypervariable in sequence and / or form of structurally defined loops), also termed complementarity determining regions (CDRs), interspersed with regions that are more conserved, termed framework regions (FRs). Each VH and VL is typically composed of three CDRs and four FRs, arranged from amino-terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4 (see also Chothia and Lesk J. Mol. Biol. 196, 901-917 (1987)). Unless otherwise stated or contradicted by context, CDR sequences herein are identified according to IMGT rules using DomainGapAlign (Lefranc MP., Nucleic Acids Research 1999;27:209-212 and Ehrenmann F., Kaas Q. and Lefranc M.-P. Nucleic Acids Res., 38, D301-307 (2010); see also internet http address "www.imgt.org". However, it should be understood that the present disclosure is not limited to CDR sequences only determined according to the IMGT rules. Thus, the HCDR1, HCDR2, and HCDR3 sequences of a heavy chain variable region (VH), e.g., SEQ ID NO: 1 or SEQ ID NO: 11, or the LCDR1, LCDR2, and LCDR3 sequences of a light chain variable region (VL), e.g., SEQ ID NO: 5 or SEQ ID NO: 15, shall encompass those CDR sequences which are determined by any method for determining CDR sequences, for example according to the IMGT rules or the Kabat rules. In addition, sequences shall also be included that represent an overlap of CDR sequences that are determined by different methods for determining CDR sequences, e.g., according to the IMGT rules and the Kabat rules.

[0104] The following table shows the results of determining CDR sequences according to the IMGT rules and the Kabat rules in relation to SEQ ID NO: 1 and SEQ ID NO: 5 or SEQ ID NO: 11 and SEQ ID NO: 15, and also shows the overlap of the determined CDR sequences.

[0105] The following table shows the results of determining CDR sequences according to the IMGT rules and the Kabat rules in relation to SEQ ID NO: 21 and SEQ ID NO: 22, and also shows the overlap of the determined CDR sequences.

[0106] The following table shows the results of determining CDR sequences according to the IMGT rules and the Kabat rules in relation to SEQ ID NO: 25 and SEQ ID NO: 26, and also shows the overlap of the determined CDR sequences. The following table shows the results of determining CDR sequences according to the IMGT rules and the Kabat rules in relation to SEQ ID NO: 27 and SEQ ID NO: 28, and also shows the overlap of the determined CDR sequences.

[0107] Unless otherwise stated or contradicted by context, reference to amino acid positions in the constant regions in the present disclosure is according to the EU-numbering (Edelman et al., Proc Natl Acad Sci USA. 1969 May;63(l):78- 85; Kabat et al., Sequences of Proteins of Immunological Interest, Fifth Edition. 1991 NIH Publication No. 91-3242).

[0108] There are five types of mammalian immunoglobulin heavy chains, i.e., a, ■, e, ■, and p which account for the different classes of antibodies, i.e., IgA, IgD, IgE, IgG, and IgM. As opposed to the heavy chains of soluble immunoglobulins, the heavy chains of membrane or surface immunoglobulins comprise a transmembrane domain and a short cytoplasmic domain at their carboxy-terminus. In mammals there are two types of light chains, i.e., lambda and kappa. The immunoglobulin chains comprise a variable region and a constant region. The constant region is essentially conserved within the different isotypes of the immunoglobulins, wherein the variable part is highly divers and accounts for antigen recognition.

[0109] The term "amino acid" and "amino acid residue" may herein be used interchangeably, and are not to be understood limiting. Amino acids are organic compounds containing amine (-NH?) and carboxyl (-COOH) functional groups, along with a side chain (R group) specific to each amino acid. In the context of the present disclosure, amino acids may be classified based on structure and chemical characteristics. Thus, classes of amino acids may be reflected in one or both of the following tables:

[0110] Table 2: Main classification based on structure and general chemical characterization of R group Table 3: Alternative Physical and Functional Classifications of Amino Acid Residues

[0111] For the purposes of the present disclosure, "variants" of an amino acid sequence (peptide, protein or polypeptide) comprise amino acid insertion variants, amino acid addition variants, amino acid deletion variants and / or amino acid substitution variants. The term "variant" includes all mutants, splice variants, posttranslationally modified variants, conformations, isoforms, allelic variants, species variants, and species homologs, in particular those which are naturally occurring. The term "variant" includes, in particular, fragments of an amino acid sequence.

[0112] Amino acid insertion variants comprise insertions of single or two or more amino acids in a particular amino acid sequence. In the case of amino acid sequence variants having an insertion, one or more amino acid residues are inserted into a particular site in an amino acid sequence, although random insertion with appropriate screening of the resulting product is also possible.

[0113] Amino acid addition variants comprise amino- and / or carboxy-terminal fusions of one or more amino acids, such as 1, 2, 3, 5, 10, 20, 30, 50, or more amino acids.

[0114] Amino acid deletion variants are characterized by the removal of one or more amino acids from the sequence, such as by removal of 1, 2, 3, 5, 10, 20, 30, 50, or more amino acids. The deletions may be in any position of the protein. Amino acid deletion variants that comprise the deletion at the N-terminal and / or C-terminal end of the protein are also called N-terminal and / or C-terminal truncation variants.

[0115] Amino acid substitution variants are characterized by at least one residue in the sequence being removed and another residue being inserted in its place. Substitution of one amino acid for another may be classified as a conservative or non-conservative substitution. Preference is given to the modifications being in positions in the amino acid sequence which are not conserved between homologous proteins or peptides and / or to replacing amino acids with other ones having similar properties. Preferably, amino acid changes in peptide and protein variants are conservative amino acid changes, i.e., substitutions of similarly charged or uncharged amino acids. A conservative amino acid change involves substitution of one of a family of amino acids which are related in their side chains. In the context of the present disclosure, a "conservative substitution" is a substitution of one amino acid with another amino acid having similar structural and / or chemical characteristics, such substitution of one amino acid residue for another amino acid residue of the same class as defined in any of the two tables above: for example, leucine may be substituted with isoleucine as they are both aliphatic, branched hydrophobes. Similarly, aspartic acid may be substituted with glutamic acid since they are both small, negatively charged residues. Naturally occurring amino acids may also be generally divided into four families: acidic (aspartate, glutamate), basic (lysine, arginine, histidine), non-polar (alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), and uncharged polar (glycine, asparagine, glutamine, cysteine, serine, threonine, tyrosine) amino acids. Phenylalanine, tryptophan, and tyrosine are sometimes classified jointly as aromatic amino acids. In one embodiment, conservative amino acid substitutions include substitutions within the following groups:

[0116] - glycine, alanine;

[0117] - valine, isoleucine, leucine;

[0118] - aspartic acid, glutamic acid;

[0119] - asparagine, glutamine;

[0120] - serine, threonine;

[0121] - lysine, arginine; and

[0122] - phenylalanine, tyrosine.

[0123] The term "amino acid corresponding to position..." and similar expressions as used herein refer to an amino acid position number in a human IgGl heavy chain. Corresponding amino acid positions in other immunoglobulins may be found by alignment with human IgGl. Thus, an amino acid or segment in one sequence that "corresponds to" an amino acid or segment in another sequence is one that aligns with the other amino acid or segment using a standard sequence alignment program such as ALIGN, ClustalW or similar, typically at default settings and has at least 50%, at least 80%, at least 90%, or at least 95% identity to a human IgGl heavy chain. It is considered well- known in the art how to align a sequence or segment in a sequence and thereby determine the corresponding position in a sequence to an amino acid position according to the present disclosure.

[0124] The term "antibody" (Ab) in the context of the present disclosure refers to an immunoglobulin molecule, a fragment of an immunoglobulin molecule, or a derivative of either thereof, which has the ability to specifically bind to an antigen (in particular an epitope on an antigen), typically under physiological conditions, preferably with a half-life of significant periods of time, such as at least about 30 minutes, at least about 45 minutes, at least about one hour, at least about two hours, at least about four hours, at least about 8 hours, at least about 12 hours, about 24 hours or more, about 48 hours or more, about 3, 4, 5, 6, 7 or more days, etc., or any other relevant functionally-defined period (such as a time sufficient to induce, promote, enhance, and / or modulate a physiological response associated with antibody binding to the antigen and / or time sufficient for the antibody to recruit an effector activity). In particular, the term "antibody" refers to a glycoprotein comprising at least two heavy (H) chains and two light (L) chains inter-connected by disulfide bonds. The term "antibody" includes monoclonal antibodies, recombinant antibodies, human antibodies, humanized antibodies, chimeric antibodies and combinations of any of the foregoing. Each heavy chain is comprised of a heavy chain variable region (VH) and a heavy chain constant region (CH). Each light chain is comprised of a light chain variable region (VL) and a light chain constant region (CL). The variable regions and constant regions are also referred to herein as variable domains and constant domains, respectively. The VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDRs), interspersed with regions that are more conserved, termed framework regions (FRs). Each VH and VL is composed of three CDRs and four FRs, arranged from amino-terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. The CDRs of a VH are termed HCDR1, HCDR2 and HCDR3 (or CDR-H1, CDR-H2 and CDR-H3), the CDRs of a VL are termed LCDR1, LCDR2 and LCDR3 (or CDR-L1, CDR-L2 and CDR-L3). The variable regions of the heavy and light chains contain a binding domain that interacts with an antigen. The constant regions of an antibody comprise the heavy chain constant region (CH) and the light chain constant region (CL), wherein CH can be further subdivided into constant domain CHI, a hinge region, and constant domains CH2 and CH3 (arranged from amino-terminus to carboxy-terminus in the following order: CHI, CH2, CH3). The constant regions of the antibodies may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g., effector cells) and components of the complement system such as Clq. Antibodies can be intact immunoglobulins derived from natural sources or from recombinant sources and can be immunoactive portions of intact immunoglobulins. Antibodies are typically tetramers of immunoglobulin molecules. Antibodies may exist in a variety of forms including, for example, polyclonal antibodies, monoclonal antibodies, Fv, Fab and F(ab)z, as well as single chain antibodies and humanized antibodies.

[0125] The variable regions of the heavy and light chains of the immunoglobulin molecule contain a binding domain that interacts with an antigen. The terms "binding region" and "antigen-binding region" are used herein interchangeably and refer to the region which interacts with the antigen and comprises both a VH region and a VL region. An antibody as used herein comprises not only monospecific antibodies, but also multispecific antibodies which comprise multiple, such as two or more, e.g., three or more, different antigen-binding regions.

[0126] As indicated above, the term antibody herein, unless otherwise stated or clearly contradicted by context, includes fragments of an antibody that are antigen-binding fragments, i.e., retain the ability to specifically bind to the antigen. It has been shown that the antigen-binding function of an antibody may be performed by fragments of a full-length antibody. Examples of antigen-binding fragments encompassed within the term "antibody" include (i) a Fab' or Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CHI domains, or a monovalent antibody as described in WO 2007 / 059782 (Genmab); (ii) F(ab’)2fragments, bivalent fragments comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting essentially of the VH and CHI domains; (iv) a Fv fragment consisting essentially of the VL and VH domains of a single arm of an antibody; (v) a dAb fragment (Ward et al., Nature 341, 544-546 (1989)), which consists essentially of a VH domain and also called domain antibodies (Holt et al; Trends Biotechnol. 2003 Nov;21(ll):484-90); (vi) camelid or Nanobody molecules (Revets et al; Expert Opin Biol Ther. 2005 Jan;5(l):lll-24); and (vii) an isolated complementarity determining region (CDR). Furthermore, although the two domains of the Fv fragment, VL and VH, are coded for by separate genes, they may be joined, using recombinant methods, by a synthetic linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain antibodies or single chain Fv (scFv), see for instance Bird et al., Science 242, 423-426 (1988) and Huston et al., PNAS USA 85, 5879-5883 (1988)). Such single chain antibodies are encompassed within the term antibody unless otherwise noted or clearly indicated by context. Although such fragments are generally included within the meaning of antibody, they collectively and each independently are unique features of the present disclosure, exhibiting different biological properties and utility. These and other useful antibody fragments in the context of the present disclosure, as well as bispecific formats of such fragments, are discussed further herein. It also should be understood that the term antibody, unless specified otherwise, also includes polyclonal antibodies, monoclonal antibodies (mAbs), antibody-like polypeptides, such as chimeric antibodies and humanized antibodies, and antibody fragments retaining the ability to specifically bind to the antigen (antigen-binding fragments) provided by any known technique, such as enzymatic cleavage, peptide synthesis, and recombinant techniques.

[0127] An antibody as generated can possess any isotype. As used herein, the term "isotype" refers to the immunoglobulin class (for instance IgG (such as IgGl, IgG2, IgG3, IgG4), IgD, IgA (such as IgAl, IgA2), IgE, IgM, or IgY) that is encoded by heavy chain constant region genes. When a particular isotype, e.g. IgGl, is mentioned herein, the term is not limited to a specific isotype sequence, e.g. a particular IgGl sequence, but is used to indicate that the antibody is closer in sequence to that isotype, e.g. IgGl, than to other isotypes. Thus, e.g. an IgGl antibody disclosed herein may be a sequence variant of a naturally-occurring IgGl antibody, including variations in the constant regions. IgGl antibodies can exist in multiple polymorphic variants termed allotypes (reviewed in Jefferis and Lefranc 2009. mAbsNo\ 1 Issue 4 1-7) any of which are suitable for use in some of the embodiments herein. Common allotypic variants in human populations are those designated by the letters a, f, n, z or combinations thereof. In any of the embodiments herein, the antibody may comprise a heavy chain Fc region comprising a human IgG Fc region. In further embodiments, the human IgG Fc region comprises a human IgGl.

[0128] The term "multispecific antibody" in the context of the present disclosure refers to an antibody having at least two different antigen-binding regions defined by different antibody sequences. In some embodiments, said different antigen-binding regions bind different epitopes on the same antigen. However, in preferred embodiments, said different antigen-binding regions bind different target antigens. In one embodiment, the multispecific antibody is a "bispecific antibody" or "bs". A multispecific antibody, such as a bispecific antibody, can be of any format, including any of the bispecific or multispecific antibody formats described herein below.

[0129] The prefixes BisGl and bsIgGl are used interchangeably herein for bispecific antibodies.

[0130] The term "full-length" when used in the context of an antibody indicates that the antibody is not a fragment, but contains all of the domains of the particular isotype normally found for that isotype in nature, e.g. the VH, CHI, CH2, CH3, hinge, VL and CL domains for an IgGl antibody.

[0131] The term "human antibody", as used herein, is intended to include antibodies having variable and framework regions derived from human germline immunoglobulin sequences and a human immunoglobulin constant domain. The human antibodies disclosed herein may include amino acid residues not encoded by human germline immunoglobulin sequences (e.g., mutations, insertions or deletions introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo). However, the term "human antibody", as used herein, is not intended to include antibodies in which CDR sequences derived from the germline of another non-human species, such as a mouse, have been grafted onto human framework sequences.

[0132] The term "chimeric antibody" as used herein, refers to an antibody wherein the variable region is derived from a non-human species (e.g. derived from rodents) and the constant region is derived from a different species, such as human. Chimeric antibodies may be generated by antibody engineering. "Antibody engineering" is a term used generically for different kinds of modifications of antibodies, and processes for antibody engineering are well-known for the skilled person. In particular, a chimeric antibody may be generated by using standard DNA techniques as described in Sambrook et al., 1989, Molecular Cloning: A laboratory Manual, New York: Cold Spring Harbor Laboratory Press, Ch. 15. Thus, the chimeric antibody may be a genetically or an enzymatically engineered recombinant antibody. It is within the knowledge of the skilled person to generate a chimeric antibody, and thus, generation of the chimeric antibody may be performed by other methods than those described herein. Chimeric monoclonal antibodies for therapeutic applications in humans are developed to reduce anticipated antibody immunogenicity of non-human antibodies, e.g. rodent antibodies. They may typically contain non-human (e.g. murine or rabbit) variable regions, which are specific for the antigen of interest, and human constant antibody heavy and light chain domains. The terms "variable region" or "variable domain" as used in the context of chimeric antibodies, refer to a region which comprises the CDRs and framework regions of both the heavy and light chains of an immunoglobulin, as described below. The term "humanized antibody" as used herein, refers to a genetically engineered non-human antibody, which contains human antibody constant domains and non-human variable domains modified to contain a high level of sequence homology to human variable domains. This can be achieved by grafting of the six non-human antibody complementarity-determining regions (CDRs), which together form the antigen binding site, onto a homologous human acceptor framework region (FR) (see WO 92 / 22653 and EP 0 629 240). In order to fully reconstitute the binding affinity and specificity of the parental antibody, the substitution of framework residues from the parental antibody (i.e. the non-human antibody) into the human framework regions (back-mutations) may be required. Structural homology modeling may help to identify the amino acid residues in the framework regions that are important for the binding properties of the antibody. Thus, a humanized antibody may comprise non-human CDR sequences, primarily human framework regions optionally comprising one or more amino acid back-mutations to the non-human amino acid sequence, and fully human constant regions. Optionally, additional amino acid modifications, which are not necessarily back-mutations, may be applied to obtain a humanized antibody with preferred characteristics, such as affinity and biochemical properties.

[0133] As used herein, a protein which is "derived from" another protein, e.g., a parent protein, means that one or more amino acid sequences of the protein are identical or similar to one or more amino acid sequences in the other or parent protein. For example, in an antibody, binding arm, antigen-binding region, constant region, or the like which is derived from another or a parent antibody, binding arm, antigen-binding region, or constant region, one or more amino acid sequences are identical or similar to those of the other or parent antibody, binding arm, antigen-binding region, or constant region. Examples of such one or more amino acid sequences include, but are not limited to, those of the VH and VL CDRs and / or one or more or all of the framework regions, VH, VL, CL, hinge, or CH regions. For example, a humanized antibody can be described herein as "derived from" a non-human parent antibody, meaning that at least the VL and VH CDR sequences are identical or similar to the VH and VL CDR sequences of said non-human parent antibody. A chimeric antibody can be described herein as being "derived from" a non- human parent antibody, meaning that typically the VH and VL sequences may be identical or similar to those of the non-human parent antibody. Another example is a binding arm or an antigen-binding region which may be described herein as being "derived from" a particular parent antibody, meaning that said binding arm or antigen- binding region typically comprises identical or similar VH and / or VL CDRs, or VH and / or VL sequences to the binding arm or antigen-binding region of said parent antibody. As described elsewhere herein, however, amino acid modifications such as mutations can be made in the CDRs, constant regions or elsewhere in the antibody, binding arm, antigen-binding region or the like, to introduce desired characteristics. When used in the context of one or more sequences derived from a first or parent protein, a "similar" amino acid sequence preferably has a sequence identity of at least about 50%, such as at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, or at least about 97%, 98% or 99%.

[0134] Non-human antibodies can be generated in a number of different species, such as mouse, rabbit, chicken, guinea pig, llama and goat.

[0135] Monoclonal antibodies can be produced by a variety of techniques, including conventional monoclonal antibody methodology, e.g., the standard somatic cell hybridization technique of Kohler and M ilstein, Nature 256: 495 (1975). Other techniques for producing monoclonal antibodies can be employed, e.g., viral or oncogenic transformation of B-lymphocytes or phage display techniques using libraries of antibody genes, and such methods are well known to a person skilled in the art. Hybridoma production in such non-human species is a very well-established procedure. Immunization protocols and techniques for isolation of splenocytes of immunized animals / non-human species for fusion are known in the art. Fusion partners (e.g., murine myeloma cells) and fusion procedures are also known.

[0136] When used herein, unless contradicted by context, the term "Fab-arm" or "arm" refers to one heavy chain-light chain pair and is used interchangeably with "half molecules" herein.

[0137] The term "binding arm comprising an antigen-binding region" means an antibody molecule or fragment that comprises an antigen-binding region. Thus, a binding arm can comprise, e.g., the six VH and VL CDR sequences, the VH and VL sequences, a Fab or Fab' fragment, or a Fab-arm.

[0138] When used herein, unless contradicted by context, the term "Fc region" refers to an antibody region consisting of the two Fc sequences of the heavy chains of an immunoglobulin, wherein said Fc sequences comprise at least a hinge region, a CH2 domain, and a CH3 domain. In one embodiment, the term "Fc region", as used herein, refers to a region comprising, in the direction from the N- to C-terminal end of the antibody, at least a hinge region, a CH2 region and a CH3 region. An Fc region of the antibody may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (such as effector cells) and components of the complement system.

[0139] In the context of the present disclosure, the term "induce Fc-mediated effector function to a lesser extent" used in relation to an antibody, including a multispecific antibody, means that the antibody induces Fc-mediated effector functions, such function in particular being selected from the list of IgG Fc receptor (FcgammaR, FcyR) binding, Clq binding, ADCC or CDC, to a lesser extent compared to a human IgGl antibody comprising (i) the same CDR sequences, in particular comprising the same first and second antigen-binding regions, as said antibody and (ii) two heavy chains comprising human IgGl hinge, CH2 and CH3 regions.

[0140] Fc-mediated effector function may be measured by binding to FcyRs, binding to Clq, or induction of Fc-mediated cross-linking via FcyRs.

[0141] The term "hinge region" as used herein refers to the hinge region of an immunoglobulin heavy chain. Thus, for example, the hinge region of a human IgGl antibody corresponds to amino acids 216-230 according to the EU numbering as set forth in Kabat (Kabat, E.A. et al., Sequences of proteins of immunological interest. 5th Edition - US Department of Health and Human Services, NIH publication No. 91-3242, pp 662,680,689 (1991). However, the hinge region may also be any of the other subtypes as described herein.

[0142] The term "CHI region" or "CHI domain" as used herein refers to the CHI region of an immunoglobulin heavy chain. Thus, for example, the CHI region of a human IgGl antibody corresponds to amino acids 118-215 according to the EU numbering as set forth in Kabat {ibid}. However, the CHI region may also be any of the other subtypes as described herein.

[0143] The term "CH2 region" or "CH2 domain" as used herein refers to the CH2 region of an immunoglobulin heavy chain. Thus, for example, the CH2 region of a human IgGl antibody corresponds to amino acids 231-340 according to the EU numbering as set forth in Kabat {ibid}. However, the CH2 region may also be any of the other subtypes as described herein. The term "CH3 region" or "CH3 domain" as used herein refers to the CH3 region of an immunoglobulin heavy chain. Thus, for example, the CH3 region of a human IgGl antibody corresponds to amino acids 341-447 according to the EU numbering as set forth in Kabat {ibid). However, the CH3 region may also be any of the other subtypes as described herein.

[0144] The term "monovalent antibody'1means in the context of the present disclosure that an antibody molecule is capable of binding a single molecule of the antigen, and thus is not capable of antigen cross-linking.

[0145] An "EpCAM antibody" or "anti-EpCAM antibody" is an antibody as described above, which binds specifically to the antigen EpCAM.

[0146] A "CD137 antibody" or "anti-CD137 antibody" is an antibody as described above, which binds specifically to the antigen CD137.

[0147] An "EpCAMxCD137 antibody" or "anti-EpCAMxCD137 antibody" is a bispecific antibody, which comprises two different antigen-binding regions, one of which binds specifically to the antigen EpCAM and one of which binds specifically to the antigen CD137.

[0148] The term "biosimilar" (e.g., of an approved reference product / biological drug) as used herein refers to a biologic product that is similar to the reference product based on data from (a) analytical studies demonstrating that the biological product is highly similar to the reference product notwithstanding minor differences in clinically inactive components; (b) animal studies (including the assessment of toxicity); and / or (c) a clinical study or studies (including the assessment of immunogenicity and pharmacokinetics or pharmacodynamics) that are sufficient to demonstrate safety, purity, and potency in one or more appropriate conditions of use for which the reference product is approved and intended to be used and for which approval is sought (e.g., that there are no clinically meaningful differences between the biological product and the reference product in terms of the safety, purity, and potency of the product). In some embodiments, the biosimilar biological product and reference product utilizes the same mechanism or mechanisms of action for the condition or conditions of use prescribed, recommended, or suggested in the proposed labeling, but only to the extent the mechanism or mechanisms of action are known for the reference product. In some embodiments, the condition or conditions of use prescribed, recommended, or suggested in the labeling proposed for the biological product have been previously approved for the reference product. In some embodiments, the route of administration, the dosage form, and / or the strength of the biological product are the same as those of the reference product. A biosimilar can be, e.g., a presently known antibody having the same primary amino acid sequence as a marketed antibody, but may be made in different cell types or by different production, purification, or formulation methods.

[0149] As used herein, the terms "binding" or "capable of binding" in the context of the binding of an antibody to a predetermined antigen or epitope typically is a binding with an affinity corresponding to a KDof about 10‘7M or less, such as about IO*8M or less, such as about 109M or less, about 10‘10M or less, or about IO-11M or even less, when determined using Bio-Layer Interferometry (BLI) or, for instance, when determined using surface plasmon resonance (SPR) technology in a BIAcore 3000 instrument using the antigen as the ligand and the antibody as the analyte. The antibody binds to the predetermined antigen with an affinity corresponding to a Ko that is at least ten- fold lower, such as at least 100-fold lower, for instance at least 1,000-fold lower, such as at least 10,000-fold lower, for instance at least 100,000-fold lower than its KD for binding to a non-specific antigen (e.g., BSA, casein) other than the predetermined antigen or a closely related antigen. The amount with which the affinity is higher is dependent on the KDof the antibody, so that when the KDof the antibody is very low (that is, the antibody is highly specific), then the degree to which the affinity for the antigen is lower than the affinity for a non-specific antigen may be at least 10,000-fold.

[0150] The term "kd" (sec1), as used herein, refers to the dissociation rate constant of a particular antibody-antigen interaction. Said value is also referred to as the kotr value.

[0151] The term "KD'1(M), as used herein, refers to the dissociation equilibrium constant of a particular antibody-antigen interaction.

[0152] Two antibodies have the "same specificity" if they bind to the same antigen and to the same epitope. Whether an antibody to be tested recognizes the same epitope as a certain antigen-binding antibody, i.e., the antibodies bind to the same epitope, may be tested by different methods well known to a person skilled in the art.

[0153] The competition between the antibodies can be detected by a cross-blocking assay. For example, a competitive ELISA assay may be used as a cross-blocking assay. E.g., target antigen may be coated on the wells of a microtiter plate and antigen-binding antibody and candidate competing test antibody may be added. The amount of the antigen-binding antibody bound to the antigen in the well indirectly correlates with the binding ability of the candidate competing test antibody that competes therewith for binding to the same epitope. Specifically, the larger the affinity of the candidate competing test antibody is for the same epitope, the smaller the amount of the antigen- binding antibody bound to the antigen-coated well. The amount of the antigen-binding antibody bound to the well can be measured by labeling the antibody with detectable or measurable labeling substances.

[0154] An antibody competing for binding to an antigen with another antibody, e.g., an antibody comprising heavy and light chain variable regions as described herein, or an antibody having the specificity for an antigen of another antibody, e.g., an antibody comprising heavy and light chain variable regions as described herein, may be an antibody comprising variants of said heavy and / or light chain variable regions as described herein, e.g. modifications in the CDRs and / or a certain degree of identity as described herein.

[0155] An "isolated multispecific antibody" as used herein is intended to refer to a multispecific antibody which is substantially free of other antibodies having different antigenic specificities (for instance an isolated bispecific antibody that specifically binds to EpCAM and CD137 is substantially free of monospecific antibodies that specifically bind to EpCAM or CD137).

[0156] The term "monoclonal antibody" as used herein refers to a preparation of antibody molecules of single molecular composition. A monoclonal antibody composition displays a single binding specificity and affinity for a particular epitope.

[0157] When used herein the term "heterodimeric interaction between the first and second CH3 regions" refers to the interaction between the first CH3 region and the second CH3 region in a first-CH3 / second-CH3 heterodimeric antibody. When used herein the term "homodimeric interactions of the first and second CH3 regions" refers to the interaction between a first CH3 region and another first CH3 region in a first-CH3 / first-CH3 homodimeric antibody and the interaction between a second CH3 region and another second CH3 region in a second-CH3 / second-CH3 homodimeric antibody.

[0158] When used herein the term "homodimeric antibody" refers to an antibody comprising two first Fab-arms or half- molecules, wherein the amino acid sequence of said Fab-arms or half-molecules is the same.

[0159] When used herein the term "heterodimeric antibody" refers to an antibody comprising a first and a second Fab- arm or half-molecule, wherein the amino acid sequence of said first and second Fab-arms or half-molecules are different. In particular, the CH3 region, or the antigen-binding region, or the CH3 region and the antigen-binding region of said first and second Fab-arms / half-molecules are different.

[0160] The term "reducing conditions" or "reducing environment" refers to a condition or an environment in which a substrate, such as a cysteine residue in the hinge region of an antibody, is more likely to become reduced than oxidized.

[0161] The present disclosure also describes multispecific antibodies, such as bispecific antibodies, comprising functional variants of the VL regions, VH regions, or one or more CDRs of the bispecific antibodies of the examples. A functional variant of a VL, VH, or CDR used in the context of a bispecific antibody still allows each antigen-binding region of the bispecific antibody to retain at least a substantial proportion (at least about 50%, 60%, 70%, 80%, 90%, 95% or more) of the affinity and / or the specificity / selecti vity of the parent bispecific antibody and in some cases such a bispecific antibody may be associated with greater affinity, selectivity and / or specificity than the parent bispecific antibody.

[0162] Such functional variants typically retain significant sequence identity to the parent blspecific antibody. The percent identity between two sequences is a function of the number of identical positions shared by the sequences ( / .e., % homology = # of identical positions / total # of positions x 100), taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences. The percent identity between two nucleotide or amino acid sequences may e.g. be determined using the algorithm of E. Meyers and W. Miller, Comput. Appl. Biosci 4, 11-17 (1988) which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. In addition, the percent identity between two amino acid sequences may be determined using the Needleman and Wunsch, J. Mol. Biol. 48, 444-453 (1970) algorithm.

[0163] In the context of the present disclosure, unless otherwise indicated, the following notations are used to describe a mutation: i) substitution of an amino acid in a given position is written as e.g. K409R which means a substitution of a lysine in position 409 of the protein with an arginine; and ii) for specific variants the specific three or one letter codes are used, including the codes Xaa and X to indicate any amino acid residue. Thus, the substitution of lysine with arginine in position 409 is designated as: K409R, and the substitution of lysine with any amino acid residue in position 409 is designated as K409X. In case of deletion of lysine in position 409 it is indicated by K409*. Exemplary variants include those which differ from the VH and / or VL and / or CDRs of the parent sequences mainly by conservative substitutions; for example, 12, such as 11, 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1 of the substitutions in the variant are conservative amino acid residue replacements.

[0164] In the context of the present disclosure, conservative substitutions may be defined by substitutions within the classes of amino acids as defined in tables 2 and 3.

[0165] Functional variants of antibody sequences described herein such as VL regions, or VH regions, or antibody sequences having a certain degree of homology or identity to antibody sequences described herein such as VL regions, or VH regions preferably comprise modifications or variations in the non-CDR sequences, while the CDR sequences preferably remain unchanged.

[0166] The term "EpCAM" as used herein, refers to epithelial cell adhesion molecule, also referred to as DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1 / 4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, BerEp4, MOC-31, Ber-Ep4. EpCAM is believed to have many different roles, and appears to play a role in cell adhesion and cancer. In one embodiment, EpCAM is human EpCAM, having UniProt accession number P16422. The sequence of human EpCAM is also shown in SEQ ID NO: 59. Amino acids 1-23 of SEQ ID NO: 59 correspond to the signal peptide of human EpCAM; while amino acids 24-265 of SEQ ID NO: 59 correspond to the extracellular domain of human EpCAM; and the remainder of the protein, i.e. from amino acids 266-288 and 289-314 of SEQ ID NO: 59 are transmembrane and cytoplasmic domain, respectively.

[0167] The term "CD137" as used herein, refers to CD137 (4-1BB), also referred to as tumor necrosis factor receptor superfamily member 9 (TNFRSF9), which is the receptor for the ligand TNFSF9 / 4-1BBL. CD137 (4-1BB) is believed to be involved in T-cell activation. Other synonyms for CD137 include, but are not limited to, 4-1BB ligand receptor, CDwl37, T-cell antigen 4-1BB homolog and T-cell antigen ILA. In one embodiment, CD137 (4-1BB) is human CD137 (4-1BB), having UniProt accession number Q07011. The sequence of human CD137 is also shown in SEQ ID NO: 37. Amino acids 1-23 of SEQ ID NO: 37 correspond to the signal peptide of human CD137; while amino acids 24- 186 of SEQ ID NO: 37 correspond to the extracellular domain of human CD137; and the remainder of the protein, i.e. from amino acids 187-213 and 214-255 of SEQ ID NO: 37 are transmembrane and cytoplasmic domain, respectively.

[0168] 'Treatment cycle" is herein defined as the time period, within the effects of separate dosages of the binding agent add on due to the pharmacodynamics of the binding agent, or in other words the time period after the subject's body is essentially cleared from the administrated binding agent. Multiple small doses in a small time window, e.g. within 2-24 few hours, such as 2-12 hours or on the same day, might be equal to a larger single dose.

[0169] In the present context, the term "treatment", "treating" or "therapeutic intervention" relates to the management and care of a subject for the purpose of combating a condition such as a disease or disorder. The term is intended to include the full spectrum of treatments for a given condition from which the subject is suffering, such as administration of the therapeutically effective compound to alleviate the symptoms or complications, to delay the progression of the disease, disorder or condition, to alleviate or relief the symptoms and complications, and / or to cure or eliminate the disease, disorder or condition as well as to prevent the condition, wherein prevention is to be understood as the management and care of an individual for the purpose of combating the disease, condition or disorder and includes the administration of the active compounds to prevent the onset of the symptoms or complications. In one embodiment, "treatment" refers to the administration of an effective amount of a therapeutically active binding agent, such as of a therapeutically active antibody, of the present disclosure with the purpose of easing, ameliorating, arresting or eradicating (curing) symptoms or disease states.

[0170] The response to treatment as well as the resistance to, failure to respond to and / or relapse from treatment with a binding agent of the present disclosure may be determined according to the Response Evaluation Criteria in Solid Tumors; version 1.1 (RECIST Criteria vl.l).

[0171] The "best overall response" is the best response recorded from the start of the treatment until disease progression / recurrence (the smallest measurements recorded since the treatment started will be used as the reference for PD). Subjects with CR or PR are considered to be objective response. Subjects with CR, PR or SD are considered to be in disease control. Subjects with NE are counted as non-responders. The best overall response is the best response recorded from the start of the treatment until disease progression / recurrence (the smallest measurements recorded since the treatment started will be used as the reference for PD). Subjects with CR, PR or SD are considered to be in disease control. Subjects with NE are counted as non-responders.

[0172] "Duration of response (DOR)" only applies to subjects whose confirmed best overall response is CR or PR and is defined as the time from the first documentation of objective tumor response (CR or PR) to the date of first PD or death due to underlying cancer.

[0173] "Progression-free survival (PFS)" is defined as the number of days from Day 1 in Cycle 1 to the first documented progression or death due to any cause.

[0174] "Overall survival (OS)" is defined as the number of days from Day 1 in Cycle 1 to death due to any cause. If a subject is not known to have died, then OS will be censored at the latest date the subject was known to be alive (on or before the cut-off date).

[0175] In the context of the present disclosure, the term "treatment regimen" refers to a structured treatment plan designed to improve and maintain health.

[0176] The term "effective amount" or "therapeutically effective amount" refers to an amount effective, at dosages and for periods of time necessary, to achieve a desired therapeutic result. A therapeutically effective amount of a binding agent, such as an antibody, like a multispecific antibody or monoclonal antibody, may vary according to factors such as the disease state, age, sex, and weight of the individual, and the ability of the binding agent to elicit a desired response in the individual. A therapeutically effective amount is also one in which any toxic or detrimental effects of the binding agent or a fragment thereof, are outweighed by the therapeutically beneficial effects. In the case that a reaction in a patient is insufficient with an initial dose, higher doses (or effectively higher doses achieved by a different, more localized route of administration) may be used. In case that unwanted side effects occur in a patient with a dose, lower doses (or effectively lower doses achieved by a different, more localized route of administration) may be used.

[0177] As used herein, the term "cancer" includes a disease characterized by aberrantly regulated cellular growth, proliferation, differentiation, adhesion, and / or migration. By "cancer cell" is meant an abnormal cell that grows by a rapid, uncontrolled cellular proliferation and continues to grow after the stimuli that initiated the new growth cease.

[0178] The term "cancer" according to the present disclosure also comprises cancer metastases. By "metastasis" is meant the spread of cancer cells from its original site to another part of the body. The formation of metastasis is a very complex process and depends on detachment of malignant cells from the primary tumor, invasion of the extracellular matrix, penetration of the endothelial basement membranes to enter the body cavity and vessels, and then, after being transported by the blood, infiltration of target organs. Finally, the growth of a new tumor, i.e. a secondary tumor or metastatic tumor, at the target site depends on angiogenesis. Tumor metastasis often occurs even after the removal of the primary tumor because tumor cells or components may remain and develop metastatic potential. In one embodiment, the term "metastasis" according to the present disclosure relates to "distant metastasis" which relates to a metastasis which is remote from the primary tumor and the regional lymph node system.

[0179] Terms such as "reduce", "inhibit", "interfere", and "negatively modulate" as used herein means the ability to cause an overall decrease, for example, of about 5% or greater, about 10% or greater, about 15% or greater, about 20% or greater, about 25% or greater, about 30% or greater, about 40% or greater, about 50% or greater, or about 75% or greater, in the level. The term "inhibit" or similar phrases includes a complete or essentially complete inhibition, i.e. a reduction to zero or essentially to zero.

[0180] Terms such as "increase" or "enhance" in one embodiment relate to an increase or enhancement by at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 80%, or at least about 100%.

[0181] "Physiological pH" as used herein refers to a pH of 7.5 or about 7.5.

[0182] As used in the present disclosure, "% by weight" refers to weight percent, which is a unit of concentration measuring the amount of a substance in grams (g) expressed as a percent of the total weight of the total composition in grams (g)-

[0183] The term "freezing" relates to the solidification of a liquid, usually with the removal of heat.

[0184] The term "lyophilizing" or "lyophilization" refers to the freeze-drying of a substance by freezing it and then reducing the surrounding pressure {e.g., below 15 Pa, such as below 10 Pa, below 5 Pa, or 1 Pa or less) to allow the frozen medium in the substance to sublimate directly from the solid phase to the gas phase. Thus, the terms "lyophilizing" and "freeze-drying" are used herein interchangeably.

[0185] The term "recombinant" in the context of the present disclosure means "made through genetic engineering". In one embodiment, a "recombinant object" in the context of the present disclosure is not occurring naturally.

[0186] The term "naturally occurring" as used herein refers to the fact that an object can be found in nature. For example, a peptide or nucleic acid that is present in an organism (including viruses) and can be isolated from a source in nature and which has not been intentionally modified by man in the laboratory is naturally occurring. The term "found in nature" means "present in nature" and includes known objects as well as objects that have not yet been discovered and / or isolated from nature, but that may be discovered and / or isolated in the future from a natural source.

[0187] According to the present disclosure, the term "peptide" comprises oligo- and polypeptides and refers to substances which comprise about two or more, about 3 or more, about 4 or more, about 6 or more, about 8 or more, about 10 or more, about 13 or more, about 16 or more, about 20 or more, and up to about 50, about 100 or about 150, consecutive amino acids linked to one another via peptide bonds. The term "protein" refers to large peptides, in particular peptides having at least about 151 amino acids, but the terms "peptide" and "protein" are used herein usually as synonyms.

[0188] A "therapeutic protein" has a positive or advantageous effect on a condition or disease state of a subject when provided to the subject in a therapeutically effective amount. In one embodiment, a therapeutic protein has curative or palliative properties and may be administered to ameliorate, relieve, alleviate, reverse, delay onset of or lessen the severity of one or more symptoms of a disease or disorder. A therapeutic protein may have prophylactic properties and may be used to delay the onset of a disease or to lessen the severity of such disease or pathological condition. The term "therapeutic protein" includes entire proteins or peptides and can also refer to therapeutically active fragments thereof. It can also include therapeutically active variants of a protein. Examples of therapeutically active proteins include, but are not limited to, antigens for vaccination and immunostimulants such as cytokines.

[0189] The term "portion" refers to a fraction. With respect to a particular structure such as an amino acid sequence or protein the term "portion" thereof may designate a continuous or a discontinuous fraction of said structure.

[0190] The terms "part" and "fragment" are used interchangeably herein and refer to a continuous element. For example, a part of a structure such as an amino acid sequence or protein refers to a continuous element of said structure. When used in context of a composition, the term "part” means a portion of the composition. For example, a part of a composition may any portion from 0.1% to 99.9% (such as 0.1%, 0.5%, 1%, 5%, 10%, 50%, 90%, or 99%) of said composition.

[0191] "Fragment", with reference to an amino acid sequence (peptide or protein), relates to a part of an amino acid sequence, i.e. a sequence which represents the amino acid sequence shortened at the N-terminus and / or C- terminus. A fragment shortened at the C-terminus (N-terminal fragment) is obtainable, e.g., by translation of a truncated open reading frame that lacks the 3'-end of the open reading frame. A fragment shortened at the N- terminus (C-terminal fragment) is obtainable, e.g., by translation of a truncated open reading frame that lacks the 5'-end of the open reading frame, as long as the truncated open reading frame comprises a start codon that serves to initiate translation. A fragment of an amino acid sequence comprises, e.g., at least 50 %, at least 60 %, at least 70 %, at least 80%, at least 90% of the amino acid residues from an amino acid sequence. A fragment of an amino acid sequence preferably comprises at least 6, in particular at least 8, at least 12, at least 15, at least 20, at least 30, at least 50, or at least 100 consecutive amino acids from an amino acid sequence.

[0192] According to the present disclosure, a part or fragment of a peptide or protein preferably has at least one functional property of the peptide or protein from which it has been derived. Such functional properties comprise a pharmacological activity, the interaction with other peptides or proteins, an enzymatic activity, the interaction with antibodies, and the selective binding of nucleic acids. E.g., a pharmacological active fragment of a peptide or protein has at least one of the pharmacological activities of the peptide or protein from which the fragment has been derived. A part or fragment of a peptide or protein preferably comprises a sequence of at least 6, in particular at least 8, at least 10, at least 12, at least 15, at least 20, at least 30 or at least 50, consecutive amino adds of the peptide or protein. A part or fragment of a peptide or protein preferably comprises a sequence of up to 8, in particular up to 10, up to 12, up to 15, up to 20, up to 30 or up to 55, consecutive amino acids of the peptide or protein.

[0193] By "variant" herein is meant an amino acid sequence that differs from a parent amino acid sequence by virtue of at least one amino acid modification. The parent amino acid sequence may be a naturally occurring or wild type (WT) amino acid sequence, or may be a modified version of a wild type amino acid sequence. Preferably, the variant amino acid sequence has at least one amino acid modification compared to the parent amino acid sequence, e.g., from 1 to about 20 amino acid modifications, and preferably from 1 to about 10 or from 1 to about 5 amino acid modifications compared to the parent.

[0194] By "wild type" or "WT" or "native" herein is meant an amino acid sequence that is found in nature, including allelic variations. A wild-type amino acid sequence, peptide or protein has an amino acid sequence that has not been intentionally modified.

[0195] Preferably the degree of similarity, preferably identity between a given amino acid sequence and an amino acid sequence which is a variant of said given amino acid sequence will be at least about 60%, 70%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%. The degree of similarity or identity is given preferably for an amino acid region which is at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90% or about 100% of the entire length of the reference amino acid sequence. For example, if the reference amino acid sequence consists of 200 amino acids, the degree of similarity or identity is given preferably for at least about 20, at least about 40, at least about 60, at least about 80, at least about 100, at least about 120, at least about 140, at least about 160, at least about 180, or about 200 amino acids, in some embodiments continuous amino acids. In some embodiments, the degree of similarity or identity is given for the entire length of the reference amino acid sequence. The alignment for determining sequence similarity, preferably sequence identity can be done with art known tools, preferably using the best sequence alignment, for example, using Align, using standard settings, preferably EMBOSS: meedle, Matrix: Blosum62, Gap Open 10.0, Gap Extend 0.5.

[0196] "Sequence similarity" indicates the percentage of amino acids that either are identical or that represent conservative amino acid substitutions. "Sequence identity" between two amino acid sequences indicates the percentage of amino acids that are identical between the sequences. "Sequence identity" between two nucleic acid sequences indicates the percentage of nucleotides that are identical between the sequences.

[0197] The terms "% identical" and "% identity" or similar terms are intended to refer, in particular, to the percentage of nucleotides or amino acids which are identical in an optimal alignment between the sequences to be compared. Said percentage is purely statistical, and the differences between the two sequences may be but are not necessarily randomly distributed over the entire length of the sequences to be compared. Comparisons of two sequences are usually carried out by comparing the sequences, after optimal alignment, with respect to a segment or "window of comparison", in order to identify local regions of corresponding sequences. The optima! alignment for a comparison may be carried out manually or with the aid of the local homology algorithm by Smith and Waterman, 1981, Ads App. Math. 2, 482, with the aid of the local homology algorithm by Neddleman and Wunsch, 1970, J. Mol. Biol. 48, 443, with the aid of the similarity search algorithm by Pearson and Lipman, 1988, Proc. Natl Acad. Sci. USA 88, 2444, or with the aid of computer programs using said algorithms (GAP, BESTFIT, FASTA, BLAST P, BLAST N and TFASTA in Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Drive, Madison, Wis.). In some embodiments, percent identity of two sequences is determined using the BLASTN or BLASTP algorithm, as available on the United States National Center for Biotechnology Information (NCBI) website (e.g., at blast.ncbi.nlm.nih.gov / Blast.cgi). In some embodiments, the algorithm parameters used for BLASTN algorithm on the NCBI website include: (i) Expect Threshold set to 10; (ii) Word Size set to 28; (iii) Max matches in a query range set to 0; (iv) Match / Mismatch Scores set to 1, -2; (v) Gap Costs set to Linear; and (vi) the filter for low complexity regions being used. In some embodiments, the algorithm parameters used for BLASTP algorithm on the NCBI website include: (i) Expect Threshold set to 10; (ii) Word Size set to 3; (iii) Max matches in a query range set to 0; (iv) Matrix set to BLOSUM62; (v) Gap Costs set to Existence: 11 Extension: 1; and (vi) conditional compositional score matrix adjustment.

[0198] Percentage identity is obtained by determining the number of identical positions at which the sequences to be compared correspond, dividing this number by the number of positions compared (e.g., the number of positions in the reference sequence) and multiplying this result by 100.

[0199] In some embodiments, the degree of similarity or identity is given for a region which is at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90% or about 100% of the entire length of the reference sequence. For example, if the reference amino acid sequence consists of 200 amino acid residues, the degree of identity is given for at least about 100, at least about 120, at least about 140, at least about 160, at least about 180, or about 200 amino acid residues, in some embodiments continuous amino acid residues. In some embodiments, the degree of similarity or identity is given for the entire length of the reference sequence.

[0200] Homologous amino acid sequences exhibit according to the present disclosure at least 40%, in particular at least 50%, at least 60%, at least 70%, at least 80%, at least 90% and preferably at least 95%, at least 98 or at least 99% identity of the amino acid residues.

[0201] The amino acid sequence variants described herein may readily be prepared by the skilled person, for example, by recombinant DNA manipulation. The manipulation of DNA sequences for preparing peptides or proteins having substitutions, additions, insertions or deletions, is described in detail in Sambrook et al. (1989), for example. Furthermore, the peptides and amino acid variants described herein may be readily prepared with the aid of known peptide synthesis techniques such as, for example, by solid phase synthesis and similar methods.

[0202] In one embodiment, a fragment or variant of an amino acid sequence (peptide or protein) is preferably a "functional fragment" or "functional variant". The term "functional fragment" or "functional variant" of an amino acid sequence relates to any fragment or variant exhibiting one or more functional properties identical or similar to those of the amino acid sequence from which it is derived, i.e., it is functionally equivalent. With respect to binding agents, one particular function is one or more binding activities displayed by the amino acid sequence from which the fragment or variant is derived. The term "functional fragment" or "functional variant", as used herein, in particular refers to a variant molecule or sequence that comprises an amino acid sequence that is altered by one or more amino acids compared to the amino acid sequence of the parent molecule or sequence and that is still capable of fulfilling one or more of the functions of the parent molecule or sequence, e.g., binding to a target antigen. In one embodiment, the modifications in the amino acid sequence of the parent molecule or sequence do not significantly affect or alter the characteristics of the molecule or sequence. In different embodiments, the function of the functional fragment or functional variant may be reduced but still significantly present, e.g., binding of the functional variant may be at least 50%, at least 60%, at least 70%, at least 80%, or at least 90% of the parent molecule or sequence. However, in other embodiments, function of the functional fragment or functional variant may be enhanced compared to the parent molecule or sequence.

[0203] An amino acid sequence (peptide, protein or polypeptide) "derived from" a designated amino acid sequence (peptide, protein or polypeptide) refers to the origin of the first amino acid sequence. Preferably, the amino acid sequence which is derived from a particular amino acid sequence has an amino acid sequence that is identical, essentially identical or homologous to that particular sequence or a fragment thereof. Amino acid sequences derived from a particular amino acid sequence may be variants of that particular sequence or a fragment thereof. For example, it will be understood by one of ordinary skill in the art that the binding agents for use herein may be altered such that they vary in sequence from the naturally occurring or native sequences from which they were derived, while retaining the desirable activity of the native sequences.

[0204] "Isolated" means altered or removed from the natural state. For example, a nucleic acid or a peptide naturally present in a living animal is not "isolated", but the same nucleic acid or peptide partially or completely separated from the coexisting materials of its natural state is "isolated". An isolated nucleic acid or protein can exist in substantially purified form, or can exist in a non-native environment such as, for example, a host cell. In one embodiment, the binding agent, e.g,, antibody, described herein is isolated. An "isolated binding agent" as used herein, is intended to refer to a binding agent which is substantially free of other binding agents having different antigenic specificities. In one embodiment, an isolated bispecific binding agent that specifically binds to EpCAM and CD137 is substantially free of monospecific antibodies that specifically bind to EpCAM or CD137. In a preferred embodiment, the binding agent used in the present disclosure is in substantially purified form.

[0205] The term "genetic modification" or simply "modification" includes the transfection of cells with nucleic acid. The term "transfection" relates to the introduction of nucleic acids, in particular RNA, into a cell. For purposes of the present disclosure, the term "transfection" also includes the introduction of a nucleic acid into a cell or the uptake of a nucleic acid by such cell, wherein the cell may be present in a subject, e.g., a patient. Thus, according to the present disclosure, a cell for transfection of a nucleic acid described herein can be present in vitro or in vivo, e.g. the cell can form part of an organ, a tissue and / or an organism of a patient. According to the present disclosure, transfection can be transient or stable. For some applications of transfection, it is sufficient if the transfected genetic material is only transiently expressed. RNA can be transfected into cells to transiently express its coded protein. Since the nucleic acid introduced in the transfection process is usually not integrated into the nuclear genome, the foreign nucleic acid will be diluted through mitosis or degraded. Cells allowing episomal amplification of nucleic acids greatly reduce the rate of dilution. If it is desired that the transfected nucleic acid actually remains in the genome of the cell and its daughter cells, a stable transfection must occur. Such stable transfection can be achieved by using virus-based systems or transposon-based systems for transfection. Generally, nucleic acid encoding antigen is transiently transfected into cells. RNA can be transfected into cells to transiently express its coded protein.

[0206] According to the present disclosure, an analog of a peptide or protein is a modified form of said peptide or protein from which it has been derived and has at least one functional property of said peptide or protein. E.g., a pharmacological active analog of a peptide or protein has at least one of the pharmacological activities of the peptide or protein from which the analog has been derived. Such modifications include any chemical modification and comprise single or multiple substitutions, deletions and / or additions of any molecules associated with the protein or peptide, such as carbohydrates, lipids and / or proteins or peptides. In one embodiment, "analogs" of proteins or peptides include those modified forms resulting from glycosylation, acetylation, phosphorylation, amidation, palmitoylation, myristoylation, isoprenylation, lipidation, alkylation, derivatization, introduction of protective / blocking groups, proteolytic cleavage or binding to an antibody or to another cellular ligand. The term "analog" also extends to all functional chemical equivalents of said proteins and peptides.

[0207] "Activation" or "stimulation", as used herein, refers to the state of an immune effector cell such as T cell that has been sufficiently stimulated to induce detectable cellular proliferation. Activation can also be associated with initiation of signaling pathways, induced cytokine production, and detectable effector functions. The term "activated immune effector cells" refers to, among other things, immune effector cells that are undergoing cell division.

[0208] The term "priming" refers to a process wherein an immune effector cell such as a T cell has its first contact with its specific antigen and causes differentiation into effector cells such as effector T cells.

[0209] The term "clonal expansion" or "expansion" refers to a process wherein a specific entity is multiplied. In the context of the present disclosure, the term is preferably used in the context of an immunological response in which immune effector cells are stimulated by an antigen, proliferate, and the specific immune effector cell recognizing said antigen is amplified. Preferably, clonal expansion leads to differentiation of the immune effector cells.

[0210] An "antigen" according to the present disclosure covers any substance or molecular structure that can bind to an antibody or T-cell receptor. The presence of an antigen in a body may elicit an immune response. Thus, "antigen" covers any substance against which an immune response or an immune mechanism is directed. This also includes situations wherein the antigen is processed into antigen peptides and an immune response or an immune mechanism is directed against one or more antigen peptides, in particular if presented in the context of MHC molecules. In particular, an "antigen" relates to any substance, preferably a peptide or protein, that reacts specifically with antibodies or T-lymphocytes (T-cells). According to the present disclosure, the term "antigen" comprises any molecule which comprises at least one epitope, such as a T cell epitope. Preferably, an antigen in the context of the present disclosure is a molecule which, optionally after processing, induces an immune reaction, which is preferably specific for the antigen (including cells expressing the antigen). In one embodiment, an antigen is a disease-associated antigen, such as a tumor antigen, a viral antigen, or a bacterial antigen, or an epitope derived from such antigen.

[0211] The term "epitope" refers to an antigenic determinant in a molecule such as an antigen, Ze., to a part in or fragment of the molecule that is recognized by the immune system, for example, that is recognized by antibodies T cells or B cells, in particular when presented in the context of MHC molecules. In one embodiment, "epitope" means a protein determinant capable of specific binding to an antibody. Epitopes usually consist of surface groupings of molecules such as amino acids or sugar side chains and usually have specific three-dimensional structural characteristics, as well as specific charge characteristics. Conformational and non-conformational epitopes are distinguished in that the binding to the former but not the latter is lost in the presence of denaturing solvents. The epitope may comprise amino acid residues directly involved in the binding and other amino acid residues, which are not directly involved in the binding, such as amino acid residues which are effectively blocked or covered by the specifically antigen-binding peptide (in other words, the amino acid residue is within the footprint of the specifically antigen-binding peptide).

[0212] An epitope of a protein preferably comprises a continuous or discontinuous portion of said protein and is preferably between about 5 and about 100, preferably between about 5 and about 50, more preferably between about 8 and about 30, most preferably between about 10 and about 25 amino acids in length, for example, the epitope may be preferably 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids in length. In one embodiment, an epitope in the context of the present disclosure is a T cell epitope.

[0213] The term "optional" or "optionally" as used herein means that the subsequently described event, circumstance or condition may or may not occur, and that the description includes instances where said event, circumstance, or condition occurs and instances in which it does not occur.

[0214] As used herein, the terms "linked", "fused", or "fusion" are used interchangeably. These terms refer to the joining together of two or more elements or components or domains.

[0215] The term "disease" (also referred to as "disorder" herein) refers to an abnormal condition that affects the body of an individual. A disease is often construed as a medical condition associated with specific symptoms and signs. A disease may be caused by factors originally from an external source, such as infectious disease, or it may be caused by internal dysfunctions, such as autoimmune diseases. In humans, "disease" is often used more broadly to refer to any condition that causes pain, dysfunction, distress, social problems, or death to the individual afflicted, or similar problems for those in contact with the individual. In this broader sense, it sometimes includes injuries, disabilities, disorders, syndromes, infections, isolated symptoms, deviant behaviors, and atypical variations of structure and function, while in other contexts and for other purposes these may be considered distinguishable categories. Diseases usually affect individuals not only physically, but also emotionally, as contracting and living with many diseases can alter one's perspective on life, and one's personality.

[0216] The term "therapeutic treatment" relates to any treatment which improves the health status and / or prolongs (increases) the lifespan of an individual. Said treatment may eliminate the disease in an individual, arrest or slow the development of a disease in an individual, inhibit or slow the development of a disease in an individual, decrease the frequency or severity of symptoms in an individual, and / or decrease the recurrence in an individual who currently has or who previously has had a disease.

[0217] The terms "prophylactic treatment" or "preventive treatment" relate to any treatment that is intended to prevent a disease from occurring in an individual. The terms "prophylactic treatment" or "preventive treatment" are used herein interchangeably. Similarly, the term "method for preventing" in the context of progression of a disease, such as progression of a tumor or cancer, relates to any method that is intended to prevent the disease from progressing in an individual.

[0218] The terms "individual" and "subject" are used herein interchangeably. They refer to a human or another mammal (e.g., mouse, rat, rabbit, dog, cat, cattle, swine, sheep, horse or primate), or any other non-mammal-animal, including birds (chicken), fish or any other animal species that can be afflicted with or is susceptible to a disease or disorder (e.g., cancer),. Unless otherwise stated, the terms "individual" and "subject" do not denote a particular age, and thus encompass adults, elderlies, children, and newborns. In embodiments of the present disclosure, the "individual" or "subject" is a "patient".

[0219] The term "patient" means an individual or subject for treatment, in particular a diseased individual or subject.

[0220] The term "polynucleotide" or "nucleic acid", as used herein, is intended to include DNA and RNA such as genomic DNA, cDNA, mRNA, recombinantly produced and chemically synthesized molecules. A nucleic acid may be single- stranded or double-stranded. RNA includes in vitro transcribed RNA (IVT RNA) or synthetic RNA.

[0221] Nucleic acids may be comprised in a vector. The term "vector" as used herein includes any vectors known to the skilled person including plasmid vectors, cosmid vectors, phage vectors such as lambda phage, viral vectors such as retroviral, adenoviral or baculoviral vectors, or artificial chromosome vectors such as bacterial artificial chromosomes (BAC), yeast artificial chromosomes (YAC), or Pl artificial chromosomes (PAC). Said vectors include expression as well as cloning vectors. Expression vectors comprise plasmids as well as viral vectors and generally contain a desired coding sequence and appropriate DNA sequences necessary for the expression of the operably linked coding sequence in a particular host organism (e.g., bacteria, yeast, plant, insect, or mammal) or in in vitro expression systems. Cloning vectors are generally used to engineer and amplify a certain desired DNA fragment and may lack functional sequences needed for expression of the desired DNA fragments.

[0222] In one embodiment of the disclosure, a nucleic acid is expressed in cells of a subject treated to provide the encoded peptide or protein. In one embodiment, the nucleic acid is transiently expressed in cells of the subject. Thus, in one embodiment, the nucleic acid is not integrated into the genome of the cells. In one embodiment, the nucleic acid is RNA, preferably in vitro transcribed RNA.

[0223] The nucleic acids described herein may be recombinant and / or isolated molecules.

[0224] In the present disclosure, the term "RNA" relates to a nucleic acid molecule which includes ribonucleotide residues. In preferred embodiments, the RNA contains all or a majority of ribonucleotide residues. As used herein, "ribonucleotide" refers to a nucleotide with a hydroxyl group at the 2'-position of a p-D-ribofuranosyl group. RNA encompasses without limitation, double stranded RNA, single stranded RNA, isolated RNA such as partially purified RNA, essentially pure RNA, synthetic RNA, recombinantly produced RNA, as well as modified RNA that differs from naturally occurring RNA by the addition, deletion, substitution and / or alteration of one or more nucleotides. Such alterations may refer to addition of non-nucleotide material to internal RNA nucleotides or to the end(s) of RNA. It is also contemplated herein that nucleotides in RNA may be non-standard nucleotides, such as chemically synthesized nucleotides or deoxynucleotides. For the present disclosure, these altered RNAs are considered analogs of naturally-occurring RNA.

[0225] In certain embodiments of the present disclosure, the RNA is messenger RNA (mRNA) that relates to a RNA transcript which encodes a peptide or protein. As established in the art, mRNA generally contains a 5' untranslated region (5'-UTR), a peptide coding region and a 3' untranslated region (3'-UTR). In some embodiments, the RNA is produced by in vitro transcription or chemical synthesis. In one embodiment, the mRNA is produced by in vitro transcription using a DNA template where DNA refers to a nucleic acid that contains deoxyribonucleotides.

[0226] In one embodiment, RNA is in vitro transcribed RNA (IVT-RNA) and may be obtained by in vitro transcription of an appropriate DNA template. The promoter for controlling transcription can be any promoter for any RNA polymerase. A DNA template for in vitro transcription may be obtained by cloning of a nucleic acid, in particular cDNA, and introducing it into an appropriate vector for in vitro transcription. The cDNA may be obtained by reverse transcription of RNA.

[0227] In one embodiment, the RNA described herein may have modified nucleosides. In some embodiments, the RNA comprises a modified nucleoside in place of at least one (e.g., every) uridine.

[0228] In some embodiments, the modified nucleoside is independently selected from pseudouridine (qi), Nl-methyl- pseudouridine (mlqj), and 5-methyl-uridine (m5U).

[0229] In some embodiments, the modified nucleoside replacing one or more uridine in the RNA may be any one or more of 3-methyl-uridine (m3U), 5-methoxy-uridine (mo5U), 5-aza-uridine, 6-aza-uridine, 2-thio-5-aza-uridine, 2-thio- uridine (s2U), 4-thio-uridine (s4U), 4-thio-pseudouridine, 2-thio-pseudouridine, 5-hydroxy-uridine (ho5U), 5- aminoallyl-uridine, 5-halo-uridine (e.g., 5-iodo-uridine or 5-bromo-uridine), uridine 5-oxyacetic acid (cmo5U), uridine 5-oxyacetic acid methyl ester (mcmo5U), 5-carboxymethyl-uridine (cm5U), 1-carboxymethyl-pseudouridine, 5-carboxyhydroxymethyl-uridine (chm5U), 5-carboxyhydroxymethyl-uridine methyl ester (mchm5U), 5- methoxycarbonylmethyl-uridine (mcm5U), 5-methoxycarbonylmethyl-2-thio-uridine (mcm5s2U), 5-aminomethyl-2- thio-uridine (nm5s2U), 5-methylaminomethyl-uridine (mnm5U), 1-ethyl-pseudouridine, 5-methylaminomethyl-2- thio-uridine (mnm5s2U), 5-methylaminomethyl-2-seleno-uridine (mnm5se2U), 5-carbamoylmethyl-uridine (ncm5U), 5-carboxymethylaminomethyl-uridine (cmnm5U), 5-carboxymethylaminomethyl-2-thio-uridine (cmnm5s2U), 5- propynyl-uridine, 1-propynyl-pseudouridine, 5-taurinomethyl-uridine (Tm5U), 1-taurinomethyl-pseudouridine, 5- taurinomethyl-2-thio-uridine(Tm5s2U), l-taurinomethyl-4-thio-pseudouridine), 5-methyl-2-thio-uridine (m5s2U), 1- methyl-4-thio-pseudouridine (m1s4qj), 4-thio-l-methyl-pseudouridine, 3-methyl-pseudouridine (m3qj), 2-thio-l- methyl-pseudouridine, 1-methyl-l-deaza-pseudouridine, 2-thio-l-methyl-l-deaza-pseudouridine, dihydrouridine (D), dihydropseudouridine, 5,6-dihydrouridine, 5-methyl-dihydrouridine (m5D), 2-thio-dihydrouridine, 2-thio- dihydropseudouridine, 2-methoxy-uridine, 2-methoxy-4-thio-uridine, 4-methoxy-pseudouridine, 4-methoxy-2-thio- pseudouridine, Nl-methyl-pseudouridine, 3-(3-amino-3-carboxypropyl)uridine (acp3U), l-methyl-3-(3-amino-3- carboxypropyl)pseudouridine (acp3ip), 5-(isopentenylaminomethyl)uridine (inm5U), 5-(isopentenylaminomethyl)-2- thio-uridine (inm5s2U), a-thio-uridine, 2'-O-methyl-uridine (Um), 5,2'-O-dimethyl-uridine (m5Um), 2'-O-methyl- pseudouridine (qim), 2-thio-2'-O-methyl-uridine (s2Um), 5-methoxycarbonylmethyl-2'-O-methyl-uridine (mcm5Um), 5-carbamoylmethyl-2'-0-methyl-uridine (ncm5Um), 5-carboxymethylaminomethyl-2'-O-methyl-uridine (cmnm5Um), 3,2'-O-dimethyl-uridine (m3Um), 5-(isopentenylaminomethyl)-2'-O-methyl-uridine (inm5Um), 1 -thio- uridine, deoxythymidine, 2'-F-ara-uridine, 2'-F-uridine, 2'-OH-ara-uridine, 5-(2-carbomethoxyvinyl) uridine, 5-[3- (l-E-propenylamino)uridine, or any other modified uridine known in the art.

[0230] In some embodiments, the RNA according to the present disclosure comprises a 5'-cap. In one embodiment, the RNA of the present disclosure does not have uncapped 5'-triphosphates. In one embodiment, the RNA may be modified by a 5*- cap analog. The term "5'-cap" refers to a structure found on the 5'-end of an mRNA molecule and generally consists of a guanosine nucleotide connected to the mRNA via a 5'- to 5'-triphosphate linkage. In one embodiment, this guanosine is methylated at the 7-position. Providing an RNA with a 5'-cap or 5'-cap analog may be achieved by in vitro transcription, in which the 5'-cap is co-transcriptionally expressed into the RNA strand, or may be attached to RNA post-transcriptionally using capping enzymes. In some embodiments, the RNA comprises a capO, capl, or cap2. According to the present disclosure, the term "capO" means the structure "m7GpppN", wherein N is any nucleoside bearing an OH moiety at position 2'. According to the present disclosure, the term "capl" means the structure "m7GpppNm", wherein Nm is any nucleoside bearing an OCH3 moiety at position 2'. According to the present disclosure, the term "cap2" means the structure "m7GpppNmNm", wherein each Nm is independently any nucleoside bearing an OCH3 moiety at position 2'.

[0231] In some embodiments, the RNA comprises a 5'-cap structure selected from the group consisting of m27'2 OG(5')ppSp(5')G (in particular its DI diastereomer), m27'3'°G(5')ppp(5')G, and m27'3‘0Gppp(mi2’ °)ApG.

[0232] In some embodiments, RNA according to the present disclosure comprises a 5'-UTR and / or a 3'-UTR. The term "untranslated region" or "UTR" relates to a region in a DNA molecule which is transcribed but is not translated into an amino acid sequence, or to the corresponding region in an RNA molecule, such as an mRNA molecule. An untranslated region (UTR) can be present 5' (upstream) of an open reading frame (5 -UTR) and / or 3' (downstream) of an open reading frame (3'-UTR). A 5 -UTR, if present, is located at the 5' end, upstream of the start codon of a protein-encoding region. A 5'-UTR is downstream of the 5'-cap (if present), e.g. directly adjacent to the 5'-cap. A 3'-UTR, if present, is located at the 3' end, downstream of the termination codon of a protein-encoding region, but the term "3 -UTR" does preferably not include the poly(A) sequence. Thus, the 3'-UTR is upstream of the poly(A) sequence (if present), e.g. directly adjacent to the poly(A) sequence.

[0233] In some embodiments, the RNA according to the present disclosure comprises a 3'-poly(A) sequence. As used herein, the term "poly(A) sequence" or "poly-A tail" refers to an uninterrupted or interrupted sequence of adenylate residues which is typically located at the 3' end of an RNA molecule. Poly(A) sequences are known to those of skill in the art and may follow the 3' UTR in the RNAs described herein. The poly(A) sequence may be of any length. In some embodiments, a poly(A) sequence comprises or consists of at least 20, at least 30, at least 40, at least 80, or at least 100 and up to 500, up to 400, up to 300, up to 200, or up to 150 nucleotides, and, in particular, about 110 nucleotides. In some embodiments, the poly(A) sequence only consists of A nucleotides. In some embodiments, the poly(A) sequence essentially consists of A nucleotides, but is interrupted by a random sequence of the four nucleotides (A, C, G, and U), as disclosed in WO 2016 / 005324 Al, hereby incorporated by reference. Such random sequence may be 5 to 50, 10 to 30, or 10 to 20 nucleotides in length. A poly(A) cassette present in the coding strand of DNA that essentially consists of dA nucleotides, but is interrupted by a random sequence having an equal distribution of the four nucleotides (dA, dC, dG, dT) and having a length of e.g. 5 to 50 nucleotides shows, on DNA level, constant propagation of plasmid DNA in E. coli and is still associated, on RNA level, with the beneficial properties with respect to supporting RNA stability and translational efficiency. In some embodiments, no nucleotides other than A nucleotides flank a poly(A) sequence at its 3' end, i.e., the poly(A) sequence is not masked or followed at its 3' end by a nucleotide other than A.

[0234] In the context of the present disclosure, the term "transcription" relates to a process, wherein the genetic code in a DNA sequence is transcribed into RNA. Subsequently, the RNA may be translated into peptide or protein.

[0235] "Encoding" refers to the inherent property of specific sequences of nucleotides in a polynucleotide, such as a gene, a cDNA, or an mRNA, to serve as templates for synthesis of other polymers and macromolecules in biological processes having either a defined sequence of nucleotides (i.e., rRNA, tRNA and mRNA) or a defined sequence of amino acids and the biological properties resulting therefrom. Thus, a gene encodes a protein if transcription and translation of mRNA corresponding to that gene produces the protein in a cell or other biological system. Both the coding strand, the nucleotide sequence of which is identical to the mRNA sequence and is usually provided in sequence listings, and the non-coding strand, used as the template for transcription of a gene or cDNA, can be referred to as encoding the protein or other product of that gene or cDNA. Similarly, RNA such as mRNA encodes a protein if translation of the RNA produces the protein in a cell or other biological system.

[0236] RNA may be naked or packaged, e.g., formulated in particles such as protein and / or lipid particles, e.g., lipid nanoparticles.

[0237] As used herein "endogenous" refers to any material from or produced inside an organism, cell, tissue or system.

[0238] As used herein, the term "exogenous" refers to any material introduced from or produced outside an organism, cell, tissue or system.

[0239] The term "expression" as used herein is defined as the transcription and / or translation of a particular nucleotide sequence.

[0240] Fc regions may have at their C-terminus a lysine. The origin of this lysine is a naturally occurring sequence found in humans from which these Fc regions are derived. During cell culture production of recombinant antibodies, this terminal lysine can be cleaved off by proteolysis by endogenous carboxypeptidase(s), resulting in a constant region having the same sequence but lacking the C-terminal lysine. For manufacturing purposes of antibodies, the DNA encoding this terminal lysine can be omitted from the sequence such that antibodies are produced without the lysine. Antibodies produced from nucleic acid sequences that either do, or do not encode a terminal lysine are substantially identical in sequence and in function since the degree of processing of the terminal lysine is typically high when e.g. using antibodies produced in CHO-based production systems (Dick, L.W. et al. Biotechnol. Bioeng. 2008;100: 1132-1143). Hence, it is understood that proteins in accordance with the disclosure, such as antibodies, can be generated with or without encoding or having a terminal lysine.

[0241] Aspects and embodiments of the present disclosure

[0242] In a first aspect, the present disclosure provides a method for treating or preventing a disease or disorder in a subject, said method comprising (i) administering to the subject binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137, and (ii) administering to the subject a PD(L)-1 axis binding antagonist, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD(L)-1 axis binding antagonist.

[0243] In some embodiments, the EpCAM is human EpCAM. In some embodiments, the CD137 is human CD137. In some embodiments, human EpCAM comprises the sequence set forth in SEQ ID NO: 59. In some embodiments, human CD137 comprises the sequence set forth in SEQ ID NO: 62.

[0244] In some embodiments, said first antigen-binding region binding to EpCAM binds to EpCAM expressed on tumor cells. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 4. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 3. In some embodiments, said first antigen- binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 2. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 64, 65, and 66, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 74, 3, and 66, respectively.

[0245] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 8. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 7. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 6. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2 and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2 and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 67, 68, and 8, respectively.

[0246] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence and a light chain variable region (VL) comprising a LCDR3 sequence, wherein the HCDR3 sequence comprises the sequence as set forth in SEQ ID NO: 4, and the LCDR3 sequence comprises the sequence as set forth in SEQ ID NO: 8.

[0247] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively.

[0248] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 64, 65, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 67, 68, and 8, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 74, 3, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively.

[0249] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 1 and / or a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 5.

[0250] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 1.

[0251] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the sequence as set forth in SEQ ID NO: 1.

[0252] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 5.

[0253] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising the sequence as set forth in SEQ ID NO: 5.

[0254] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 1 and the VL comprises a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 5. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 80% identity to the sequence as set forth in SEQ ID NO: 1 and the VL comprises a sequence having at least 80% identity to the sequence as set forth in SEQ ID NO: 5, In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 90% identity to the sequence as set forth in SEQ ID NO: 1 and the VL comprises a sequence having at least 90% identity to the sequence as set forth in SEQ ID NO: 5. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 95% identity to the sequence as set forth in SEQ ID NO: 1 and the VL comprises a sequence having at least 95% identity to the sequence as set forth in SEQ ID NO: 5.

[0255] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 1 and the VL comprises the sequence as set forth in SEQ ID NO: 5. In some embodiments, said first antigen-binding region binding to EpCAM comprises heavy and light chain variable regions of an antibody which competes for EpCAM binding with and / or has the specificity for EpCAM of an antibody comprising a heavy chain variable region (VH) and / or a light chain variable region (VL) as set forth above.

[0256] In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 14. In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 13. In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 12. In some embodiments, said second antigen- binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively. In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 69, 70, and 71, respectively. In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 75, 13, and 71, respectively.

[0257] In some embodiments, said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a LCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 18. In some embodiments, said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a LCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 17. In some embodiments, said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a LCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 16. In some embodiments, said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively. In some embodiments, said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 72, 73 and 18, respectively.

[0258] In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR3 sequence and a light chain variable region (VL) comprising a LCDR3 sequence, wherein the HCDR3 sequence comprises the sequence as set forth in SEQ ID NO: 14, and the LCDR3 sequence comprises the sequence as set forth in SEQ ID NO: 18.

[0259] In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

[0260] In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 69, 70, and 71, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 72, 73 and 18, respectively.

[0261] In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 75, 13, and 71, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

[0262] In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 11 and / or a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 15.

[0263] In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 11.

[0264] In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising the sequence as set forth in SEQ ID NO: 11.

[0265] In some embodiments, said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 15.

[0266] In some embodiments, said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising the sequence as set forth in SEQ ID NO: 15.

[0267] In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 11 and the VL comprises a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 15. In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 80% identity to the sequence as set forth in SEQ ID NO: 11 and the VL comprises a sequence having at least 80% identity to the sequence as set forth in SEQ ID NO: 15. In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 90% identity to the sequence as set forth in SEQ ID NO: 11 and the VL comprises a sequence having at least 90% identity to the sequence as set forth in SEQ ID NO: 15. In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 95% identity to the sequence as set forth in SEQ ID NO: 11 and the VL comprises a sequence having at least 95% identity to the sequence as set forth in SEQ ID NO: 15. In some embodiments, said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 11 and the VL comprises the sequence as set forth in SEQ ID NO: 15.

[0268] In some embodiments, said second antigen-binding region binding to CD137 comprises heavy and light chain variable regions of an antibody which competes for CD137 binding with and / or has the specificity for CD137 of an antibody comprising a heavy chain variable region and / or a light chain variable region as set forth above.

[0269] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence as set forth in SEQ ID NO: 4, and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR3 sequence as set forth in SEQ ID NO: 14.

[0270] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR3 sequence as set forth in SEQ ID NO: 8, and b) said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a LCDR3 sequence as set forth in SEQ ID NO: 18.

[0271] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence as set forth in SEQ ID NO: 4, and a light chain variable region (VL) comprising a LCDR3 sequence as set forth in SEQ ID NO: 8, and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR3 sequence as set forth in SEQ ID NO: 14, and a light chain variable region (VL) comprising a LCDR3 sequence, as set forth in SEQ ID NO: 18.

[0272] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

[0273] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 64, 65, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 67, 68, and 8, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 69, 70, and 71, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 72, 73 and 18, respectively.

[0274] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 74, 3, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 75, 13, and 71, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

[0275] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 1 and a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 5; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 11 and a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 15.

[0276] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 1 and the VL comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 5; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 11 and the VL comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 15.

[0277] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 1 and the VL comprises the sequence as set forth in SEQ ID NO: 5; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 11 and the VL comprises the sequence as set forth in SEQ ID NO: 15.

[0278] In some embodiments, the binding agent is an antibody comprising a first binding arm comprising said first antigen- binding region binding to EpCAM and a second binding arm comprising said second antigen-binding region binding to CD137, wherein the first binding arm comprises i) a polypeptide comprising a first heavy chain variable region (VH) and a first heavy chain constant region (CH), and ii) a polypeptide comprising a first light chain variable region (VL) and a first light chain constant region (CL), wherein the first VH comprises first HCDR1, HCDR2, and HCDR3 sequences and the first VL comprises first LCDR1, LCDR2, and LCDR3 sequences, wherein the first HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively, and the first LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively; and the second binding arm comprises iii) a polypeptide comprising a second heavy chain variable region (VH) and a second heavy chain constant region (CH), and iv) a polypeptide comprising a second light chain variable region (VL) and a second light chain constant region (CL), wherein the second VH comprises second HCDR1, HCDR2, and HCDR3 sequences and the second VL comprises second LCDR1, LCDR2, and LCDR3 sequences, wherein the second HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the second LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 16, 17, and 18, respectively; wherein positions L234, L235, and G236 in a human IgGI heavy chain according to EU numbering are F, E, and R, respectively, in the first CH and positions L234, L235, and D265 in a human IgGI heavy chain according to EU numbering are F, E, and A in the second CH; and wherein the amino acid in the position corresponding to F405 in a human IgGI heavy chain according to EU numbering is L in the first CH and the amino acid in the position corresponding to K409 in a human IgGI heavy chain according to EU numbering is R in the second CH.

[0279] In some embodiments, the binding agent comprises i) a first heavy chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 9, ii) a first light chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 10, iii) a second heavy chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 19; and iv) a second light chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 20.

[0280] In some embodiments, the binding agent comprises i) a first heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 9, ii) a first light chain comprising the amino acid sequence set forth in SEQ ID NO: 10, iii) a second heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 19; and iv) a second light chain comprising the amino acid sequence set forth in SEQ ID NO: 20.

[0281] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 78. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 77. In some embodiments, said first antigen- binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 76. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 76, 77, and 78, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 82, 83, and 84, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 87, 77, and 84, respectively.

[0282] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 81. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 80. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 79. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2 and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 79, 80, and 81, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2 and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 85, 86, and 81, respectively.

[0283] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence and a light chain variable region (VL) comprising a LCDR3 sequence, wherein the HCDR3 sequence comprises the sequence as set forth in SEQ ID NO: 78, and the LCDR3 sequence comprises the sequence as set forth in SEQ ID NO: 81.

[0284] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 76, 77, and 78, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 79, 80, and 81, respectively.

[0285] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 82, 83, and 84, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 85, 86, and 81, respectively.

[0286] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 87, 77, and 84, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 79, 80, and 81, respectively.

[0287] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 21, positions 1 to 116 and / or a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 22, positions 1 to 112.

[0288] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 21, positions 1 to 116.

[0289] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the sequence as set forth in SEQ ID NO: 21, positions 1 to 116.

[0290] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 22, positions 1 to 112.

[0291] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising the sequence as set forth in SEQ ID NO: 22, positions 1 to 112.

[0292] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 21, positions 1 to 116 and the VL comprises a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 22, positions 1 to 112. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 80% identity to the sequence as set forth in SEQ ID NO: 21, positions 1 to 116 and the VL comprises a sequence having at least 80% identity to the sequence as set forth in SEQ ID NO: 22, positions 1 to 112. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 90% identity to the sequence as set forth in SEQ ID NO: 21, positions 1 to 116 and the VL comprises a sequence having at least 90% identity to the sequence as set forth in SEQ ID NO: 22, positions 1 to 112. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 95% identity to the sequence as set forth in SEQ ID NO: 21, positions 1 to 116 and the VL comprises a sequence having at least 95% identity to the sequence as set forth in SEQ ID NO: 22, positions 1 to 112.

[0293] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 21, positions 1 to 116 and the VL comprises the sequence as set forth in SEQ ID NO: 22, positions 1 to 112.

[0294] In some embodiments, said first antigen-binding region binding to EpCAM comprises heavy and light chain variable regions of an antibody which competes for EpCAM binding with and / or has the specificity for EpCAM of an antibody comprising a heavy chain variable region (VH) and / or a light chain variable region (VL) as set forth above.

[0295] In some embodiments, said second antigen-binding region binding to CD137 is as set forth above.

[0296] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence as set forth in SEQ ID NO: 78, and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR3 sequence as set forth in SEQ ID NO: 14.

[0297] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR3 sequence as set forth in SEQ ID NO: 81, and b) said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a LCDR3 sequence as set forth in SEQ ID NO: 18.

[0298] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence as set forth in SEQ ID NO: 78, and a light chain variable region (VL) comprising a LCDR3 sequence as set forth in SEQ ID NO: 81, and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR3 sequence as set forth in SEQ ID NO: 14, and a light chain variable region (VL) comprising a LCDR3 sequence, as set forth in SEQ ID NO: 18.

[0299] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 76, 77, and 78, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 79, 80, and 81, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively. In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 82, 83, and 84, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 85, 86, and 81, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 69, 70, and 71, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 72, 73 and 18, respectively.

[0300] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 87, 77, and 84, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 79, 80, and 81, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 75, 13, and 71, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

[0301] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 21, positions 1 to 116 and a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 22, positions 1 to 112; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 11 and a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 15.

[0302] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 21, positions 1 to 116 and the VL comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 22, positions 1 to 112; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 11 and the VL comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 15. In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 21, positions 1 to 116 and the VL comprises the sequence as set forth in SEQ ID NO: 22, positions 1 to 112; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 11 and the VL comprises the sequence as set forth in SEQ ID NO: 15.

[0303] In some embodiments, the binding agent is an antibody comprising a first binding arm comprising said first antigen- binding region binding to EpCAM and a second binding arm comprising said second antigen-binding region binding to CD137, wherein the first binding arm comprises i) a polypeptide comprising a first heavy chain variable region (VH) and a first heavy chain constant region (CH), and ii) a polypeptide comprising a first light chain variable region (VL) and a first light chain constant region (CL), wherein the first VH comprises first HCDR1, HCDR2, and HCDR3 sequences and the first VL comprises first LCDR1, LCDR2, and LCDR3 sequences, wherein the first HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 76, 77, and 78, respectively, and the first LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 79, 80, and 81, respectively; and the second binding arm comprises iii) a polypeptide comprising a second heavy chain variable region (VH) and a second heavy chain constant region (CH), and iv) a polypeptide comprising a second light chain variable region (VL) and a second light chain constant region (CL), wherein the second VH comprises second HCDR1, HCDR2, and HCDR3 sequences and the second VL comprises second LCDR1, LCDR2, and LCDR3 sequences, wherein the second HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the second LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 16, 17, and 18, respectively; wherein positions L234, L235, and G236 in a human IgGI heavy chain according to El) numbering are F, E, and R, respectively, in the first CH and positions L234, L235, and D265 in a human IgGI heavy chain according to EU numbering are F, E, and A in the second CH; and wherein the amino acid in the position corresponding to F405 in a human IgGI heavy chain according to EU numbering is L in the first CH and the amino acid in the position corresponding to K409 in a human IgGI heavy chain according to EU numbering is R in the second CH.

[0304] In some embodiments, the binding agent comprises i) a first heavy chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 21, ii) a first light chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 22, iii) a second heavy chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 19; and iv) a second light chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 20.

[0305] In some embodiments, the binding agent comprises i) a first heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 21, ii) a first light chain comprising the amino acid sequence set forth in SEQ ID NO: 22, iii) a second heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 19; and iv) a second light chain comprising the amino acid sequence set forth in SEQ ID NO: 20.

[0306] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 90. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 89. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 88. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2 and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 88, 89, and 90, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2 and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 91, 92, and 90, respectively.

[0307] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence and a light chain variable region (VL) comprising a LCDR3 sequence, wherein the HCDR3 sequence comprises the sequence as set forth in SEQ ID NO: 4, and the LCDR3 sequence comprises the sequence as set forth in SEQ ID NO: 90.

[0308] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 88, 89, and 90, respectively.

[0309] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 64, 65, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 91, 92, and 90, respectively.

[0310] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 74, 3, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 88, 89, and 90, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 25, positions 1 to 115 and / or a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 26, positions 1 to 111.

[0311] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 25, positions 1 to 115.

[0312] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the sequence as set forth in SEQ ID NO: 25, positions 1 to 115.

[0313] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 26, positions 1 to 111.

[0314] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising the sequence as set forth in SEQ ID NO: 26, positions 1 to 111.

[0315] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 25, positions 1 to 115 and the VL comprises a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth In SEQ ID NO: 26, positions 1 to 111. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 80% identity to the sequence as set forth in SEQ ID NO: 25, positions 1 to 115 and the VL comprises a sequence having at least 80% identity to the sequence as set forth in SEQ ID NO: 26, positions 1 to 111. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 90% identity to the sequence as set forth in SEQ ID NO: 25, positions 1 to 115 and the VL comprises a sequence having at least 90% identity to the sequence as set forth in SEQ ID NO: 26, positions 1 to 111. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 95% identity to the sequence as set forth in SEQ ID NO: 25, positions 1 to 115 and the VL comprises a sequence having at least 95% identity to the sequence as set forth in SEQ ID NO: 26, positions 1 to 111.

[0316] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 25, positions 1 to 115 and the VL comprises the sequence as set forth in SEQ ID NO: 26, positions 1 to 111.

[0317] In some embodiments, said first antigen-binding region binding to EpCAM comprises heavy and light chain variable regions of an antibody which competes for EpCAM binding with and / or has the specificity for EpCAM of an antibody comprising a heavy chain variable region (VH) and / or a light chain variable region (VL) as set forth above. In some embodiments, said second antigen-binding region binding to CD137 is as set forth above.

[0318] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR3 sequence as set forth in SEQ ID NO: 90, and b) said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a LCDR3 sequence as set forth in SEQ ID NO: 18.

[0319] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence as set forth in SEQ ID NO: 4, and a light chain variable region (VL) comprising a LCDR3 sequence as set forth in SEQ ID NO: 90, and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR3 sequence as set forth in SEQ ID NO: 14, and a light chain variable region (VL) comprising a LCDR3 sequence, as set forth in SEQ ID NO: 18.

[0320] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 88, 89, and 90, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

[0321] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 64, 65, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 91, 92, and 90, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 69, 70, and 71, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 72, 73 and 18, respectively.

[0322] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 74, 3, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 88, 89, and 90, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 75, 13, and 71, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

[0323] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 25, positions 1 to 115 and a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 26, positions 1 to 111; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 11 and a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 15.

[0324] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 25, positions 1 to 115 and the VL comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 26, positions 1 to 111; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 11 and the VL comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 15.

[0325] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 25, positions 1 to 115 and the VL comprises the sequence as set forth in SEQ ID NO: 26, positions 1 to 111; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 11 and the VL comprises the sequence as set forth in SEQ ID NO: 15.

[0326] In some embodiments, the binding agent is an antibody comprising a first binding arm comprising said first antigen- binding region binding to EpCAM and a second binding arm comprising said second antigen-binding region binding to CD137, wherein the first binding arm comprises i) a polypeptide comprising a first heavy chain variable region (VH) and a first heavy chain constant region (CH), and ii) a polypeptide comprising a first light chain variable region (VL) and a first light chain constant region (CL), wherein the first VH comprises first HCDR1, HCDR2, and HCDR3 sequences and the first VL comprises first LCDR1, LCDR2, and LCDR3 sequences, wherein the first HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively, and the first LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 88, 89, and 90, respectively; and the second binding arm comprises iii) a polypeptide comprising a second heavy chain variable region (VH) and a second heavy chain constant region (CH), and iv) a polypeptide comprising a second light chain variable region (VL) and a second light chain constant region (CL), wherein the second VH comprises second HCDR1, HCDR2, and HCDR3 sequences and the second VL comprises second LCDR1, LCDR2, and LCDR3 sequences, wherein the second HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the second LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 16, 17, and 18, respectively; wherein positions L234, L235, and G236 in a human IgGI heavy chain according to EU numbering are F, E, and R, respectively, in the first CH and positions L234, L235, and D265 in a human IgGI heavy chain according to EU numbering are F, E, and A in the second CH; and wherein the amino acid in the position corresponding to F405 in a human IgGI heavy chain according to EU numbering is L in the first CH and the amino acid in the position corresponding to K409 in a human IgGI heavy chain according to EU numbering is R in the second CH.

[0327] In some embodiments, the binding agent comprises i) a first heavy chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 25, ii) a first light chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 26, iii) a second heavy chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 19; and iv) a second light chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 20.

[0328] In some embodiments, the binding agent comprises i) a first heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 25, ii) a first light chain comprising the amino acid sequence set forth in SEQ ID NO: 26, iii) a second heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 19; and iv) a second light chain comprising the amino acid sequence set forth in SEQ ID NO: 20.

[0329] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 4. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 93. In some embodiments, said first antigen- binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 2. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 93, and 4, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 64, 96, and 66, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 74, 93, and 66, respectively.

[0330] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 8. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 95. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 94. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2 and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 94, 95, and 8, respectively. In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2 and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 97, 98, and 8, respectively.

[0331] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 93, and 4, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 94, 95, and 8, respectively.

[0332] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 64, 96, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 97, 98, and 8, respectively.

[0333] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 74, 93, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 94, 95, and 8, respectively.

[0334] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 27, positions 1 to 115 and / or a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 28, positions 1 to 108. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 27, positions 1 to 115.

[0335] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the sequence as set forth in SEQ ID NO: 27, positions 1 to 115.

[0336] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 28, positions 1 to 108.

[0337] In some embodiments, said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising the sequence as set forth in SEQ ID NO: 28, positions 1 to 108.

[0338] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 27, positions 1 to 115 and the VL comprises a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 28, positions 1 to 108. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 80% identity to the sequence as set forth in SEQ ID NO: 27, positions 1 to 115 and the VL comprises a sequence having at least 80% identity to the sequence as set forth in SEQ ID NO: 28, positions 1 to 108. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 90% identity to the sequence as set forth in SEQ ID NO: 27, positions 1 to 115 and the VL comprises a sequence having at least 90% identity to the sequence as set forth in SEQ ID NO: 28, positions 1 to 108. In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises a sequence having at least at least 95% identity to the sequence as set forth in SEQ ID NO: 27, positions 1 to 115 and the VL comprises a sequence having at least 95% identity to the sequence as set forth in SEQ ID NO: 28, positions 1 to 108.

[0339] In some embodiments, said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 27, positions 1 to 115 and the VL comprises the sequence as set forth in SEQ ID NO: 28, positions 1 to 108.

[0340] In some embodiments, said first antigen-binding region binding to EpCAM comprises heavy and light chain variable regions of an antibody which competes for EpCAM binding with and / or has the specificity for EpCAM of an antibody comprising a heavy chain variable region (VH) and / or a light chain variable region (VL) as set forth above.

[0341] In some embodiments, said second antigen-binding region binding to CD137 is as set forth above.

[0342] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 93, and 4, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 94, 95, and 8, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

[0343] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 64, 96, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 97, 98, and 8, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 69, 70, and 71, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 72, 73 and 18, respectively.

[0344] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 74, 93, and 66, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 94, 95, and 8, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 75, 13, and 71, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

[0345] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 27, positions 1 to 115 and a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 28, positions 1 to 108; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising the HCDR1, HCDR2, and HCDR3 sequences of SEQ ID NO: 11 and a light chain variable region (VL) comprising the LCDR1, LCDR2, and LCDR3 sequences of SEQ ID NO: 15.

[0346] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 27, positions 1 to 115 and the VL comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 28, positions 1 to 108; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 11 and the VL comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 15.

[0347] In some embodiments, a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 27, positions 1 to 115 and the VL comprises the sequence as set forth in SEQ ID NO: 28, positions 1 to 108; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 11 and the VL comprises the sequence as set forth in SEQ ID NO: 15.

[0348] In some embodiments, the binding agent is an antibody comprising a first binding arm comprising said first antigen- binding region binding to EpCAM and a second binding arm comprising said second antigen-binding region binding to CD137, wherein the first binding arm comprises i) a polypeptide comprising a first heavy chain variable region (VH) and a first heavy chain constant region (CH), and ii) a polypeptide comprising a first light chain variable region (VL) and a first light chain constant region (CL), wherein the first VH comprises first HCDR1, HCDR2, and HCDR3 sequences and the first VL comprises first LCDR1, LCDR2, and LCDR3 sequences, wherein the first HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 93, and 4, respectively, and the first LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 94, 95, and 8, respectively; and the second binding arm comprises iii) a polypeptide comprising a second heavy chain variable region (VH) and a second heavy chain constant region (CH), and iv) a polypeptide comprising a second light chain variable region (VL) and a second light chain constant region (CL), wherein the second VH comprises second HCDR1, HCDR2, and HCDR3 sequences and the second VL comprises second LCDR1, LCDR2, and LCDR3 sequences, wherein the second HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the second LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 16, 17, and 18, respectively; wherein positions L234, L235, and G236 in a human IgGI heavy chain according to EU numbering are F, E, and R, respectively, in the first CH and positions L234, L235, and D265 in a human IgGI heavy chain according to EU numbering are F, E, and A in the second CH; and wherein the amino acid in the position corresponding to F405 in a human IgGI heavy chain according to EU numbering is L in the first CH and the amino acid in the position corresponding to K409 in a human IgGI heavy chain according to EU numbering is R in the second CH.

[0349] In some embodiments, the binding agent comprises i) a first heavy chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 27, ii) a first light chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 28, iii) a second heavy chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 19; and iv) a second light chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 20.

[0350] In some embodiments, the binding agent comprises i) a first heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 27, ii) a first light chain comprising the amino acid sequence set forth in SEQ ID NO: 28, iii) a second heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 19; and iv) a second light chain comprising the amino acid sequence set forth in SEQ ID NO: 20.

[0351] In some embodiments, said first antigen-binding region binding to EpCAM comprises heavy and light chain variable regions of an antibody which competes for EpCAM binding with and / or has the specificity for EpCAM of an antibody comprising a heavy chain variable region or a light chain variable region, or a combination thereof of a first antigen- binding region binding to EpCAM as set forth above and said second antigen-binding region binding to CD137 comprises heavy and light chain variable regions of an antibody which competes for CD137 binding with and / or has the specificity for CD137 of an antibody comprising a heavy chain variable region or a light chain variable region, or a combination thereof of a second antigen-binding region binding to CD137 as set forth above.

[0352] In some embodiments, a variable region comprises three complementarity determining regions (CDR1, CDR2, and CDR3) and four framework regions (FR1, FR2, FR3, and FR4).

[0353] In some embodiments, said complementarity determining regions and said framework regions are arranged from amino-terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4.

[0354] In some embodiments, the binding agent is in the format of a full-length antibody or an antibody fragment.

[0355] In some embodiments, the binding agent is a multispecific such as a bispecific binding agent.

[0356] In some embodiments, the binding agent is a multispecific such as a bispecific antibody.

[0357] In some embodiments, the binding agent comprises (i) a first heavy chain variable region (VH) and a first light chain variable region (VL), wherein said first heavy chain variable region (VH) and said first light chain variable region (VL) form said first antigen-binding region binding to EpCAM; and

[0358] (ii) a second heavy chain variable region (VH) and a second light chain variable region (VL), wherein said second heavy chain variable region (VH) and said second light chain variable region (VL) form said second antigen- binding region binding to CD137.

[0359] In some embodiments, the binding agent comprises i) a polypeptide comprising a first heavy chain variable region (VH) and a first heavy chain constant region (CH), and a polypeptide comprising a first light chain variable region (VL) and a first light chain constant region (CL), wherein said first heavy chain variable region (VH) and said first light chain variable region (VL) form said first antigen-binding region binding to EpCAM; and ii) a polypeptide comprising a second heavy chain variable region (VH) and a second heavy chain constant region (CH), and a polypeptide comprising a second light chain variable region (VL) and a second light chain constant region (CL), wherein said second heavy chain variable region (VH) and said second light chain variable region (VL) form said second antigen-binding region binding to CD137.

[0360] In some embodiments, the binding agent is an antibody comprising a first binding arm comprising said first antigen- binding region binding to EpCAM and a second binding arm comprising said second antigen-binding region binding to CD137, wherein the first binding arm comprises i) a polypeptide comprising a first heavy chain variable region (VH) and a first heavy chain constant region (CH), and ii) a polypeptide comprising a first light chain variable region (VL) and a first light chain constant region (CL); and the second binding arm comprises iii) a polypeptide comprising a second heavy chain variable region (VH) and a second heavy chain constant region (CH), and iv) a polypeptide comprising a second light chain variable region (VL) and a second light chain constant region (CL).

[0361] In some embodiments, the binding agent is an antibody comprising a first binding arm comprising said first antigen- binding region binding to EpCAM and a second binding arm comprising said second antigen-binding region binding to CD137, wherein the first binding arm comprises i) a first heavy chain comprising a first heavy chain variable region (VH) and a first heavy chain constant region (CH), and ii) a first light chain comprising a first light chain variable region (VL) and a first light chain constant region (CL); and the second binding arm comprises i) a second heavy chain comprising a second heavy chain variable region (VH) and a second heavy chain constant region (CH); and ii) a second light chain comprising a second light chain variable region (VL) and a second light chain constant region (CL). In some embodiments, the first binding arm is derived from a full-length antibody. In some embodiments, the first binding arm is derived from a monoclonal antibody. In some embodiments, the first binding arm is derived from a full-length IgGl, A (lambda) or IgGl, K (kappa) antibody. In some embodiments, the second binding arm is derived from a full-length antibody. In some embodiments, the second binding arm is derived from a monoclonal antibody. In some embodiments, the second binding arm is derived from a full-length IgGl, A (lambda) or IgGl, K (kappa) antibody. In some embodiments, the first and second binding arms are derived from full-length antibodies, such as from full-length IgGl, A (lambda) or IgGl, K (kappa) antibodies. In some embodiments, the first and second binding arms are derived from monoclonal antibodies.

[0362] In some embodiments, each of the first and second heavy chain constant regions (CHs) comprises one or more of a constant heavy chain 1 (CHI) region, a hinge region, a constant heavy chain 2 (CH2) region and a constant heavy chain 3 (CH3) region, preferably at least a hinge region, a CH2 region and a CH3 region.

[0363] In some embodiments, each of the first and second heavy chain constant regions (CHs) comprises a CH3 region and wherein the two CH3 regions comprise asymmetrical mutations.

[0364] In some embodiments, in said first heavy chain constant region (CH) at least one of the amino acids in a position corresponding to a position selected from the group consisting of T366, L368, K370, D399, F405, Y407, and K409 in a human IgGl heavy chain according to EU numbering has been substituted, and in said second heavy chain constant region (CH) at least one of the amino acids in a position corresponding to a position selected from the group consisting of T366, L368, K370, D399, F405, Y407, and K409 in a human IgGl heavy chain according to EU numbering has been substituted, and wherein said first and said second heavy chains are not substituted in the same positions.

[0365] In some embodiments, (i) the amino acid in the position corresponding to F405 in a human IgGl heavy chain according to EU numbering is L in said first heavy chain constant region (CH), and the amino acid in the position corresponding to K409 in a human IgGl heavy chain according to EU numbering is R in said second heavy chain constant region (CH), or (ii) the amino acid in the position corresponding to K409 in a human IgGl heavy chain according to EU numbering is R in said first heavy chain constant region (CH), and the amino acid in the position corresponding to F405 in a human IgGl heavy chain according to EU numbering is L in said second heavy chain constant region (CH).

[0366] In some embodiments, said binding agent induces Fc-mediated effector function to a lesser extent compared to another antibody comprising the same first and second antigen binding regions and two heavy chain constant regions (CHs) comprising human IgGl hinge, CH2 and CH3 regions.

[0367] In some embodiments, said first and second heavy chain constant regions (CHs) are modified so that the antibody induces Fc-mediated effector function to a lesser extent compared to an antibody which is identical except for comprising non-modified first and second heavy chain constant regions (CHs).

[0368] In some embodiments, each of said non-modified first and second heavy chain constant regions (CHs) comprises the amino acid sequence set forth in SEQ ID NO: 47. In some embodiments, said Fc-mediated effector function is measured by binding to Fey receptors, binding to Clq, or induction of Fc-mediated crosslinking of Fey receptors.

[0369] In some embodiments, said Fc-mediated effector function is measured by binding to Clq.

[0370] In some embodiments, said first and second heavy chain constant regions have been modified so that binding of Clq to said antibody is reduced compared to a wild-type antibody, preferably reduced by at least 70%, at least 80%, at least 90%, at least 95%, at least 97%, or 100%, wherein Clq binding is preferably determined by ELISA.

[0371] In some embodiments, in at least one of said first and second heavy chain constant regions (CHs), one or more amino acids in the positions corresponding to positions L234, L235, D265, N297, P331, and G236 in a human IgGl heavy chain according to EU numbering, are not L, L, D, N, P, and G, respectively.

[0372] In some embodiments, the positions corresponding to positions L234 and L235 in a human IgGl heavy chain according to EU numbering are F and E, respectively, in said first and second heavy chains.

[0373] In some embodiments, the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A, respectively, in said first and / or second heavy chain constant regions (HCs) and / or the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering are F, E, and R, respectively, in said first and / or second heavy chain constant regions (HCs).

[0374] In some embodiments,

[0375] (i) the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A, respectively, in both said first and second heavy chain constant regions;

[0376] (ii) the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering are F, E, and R, respectively, in both said first and second heavy chain constant regions; or

[0377] (iii) the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A, respectively, in one of said first and second heavy chain constant regions and the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering are F, E, and R, respectively, in the other of said first and second heavy chain constant regions.

[0378] In some embodiments, the positions corresponding to positions L234 and L235 in a human IgGl heavy chain according to EU numbering of both the first and second heavy chain constant regions are F and E, respectively, and wherein (i) the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is L, and the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is R, or (ii) the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is R, and the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is L.

[0379] In some embodiments, the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A, respectively, in said second heavy chain constant region (HC) and the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering are F, E, and R, respectively, in said first heavy chain constant region (HC), and wherein (i) the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is L, and the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is R, or (ii) the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is R, and the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is L.

[0380] In some embodiments, the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A, respectively, in said second heavy chain constant region (HC) and the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering are F, E, and R, respectively, in said first heavy chain constant region (HC), and wherein the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is L, and the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is R.

[0381] In some embodiments, the constant region of said first and / or second heavy chain comprises an amino acid sequence an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 47.

[0382] In some embodiments, a) the constant region of said first heavy chain comprises the amino acid sequence set forth in SEQ ID NO: 54; and b) the constant region of said second heavy chain comprises the amino acid sequence set forth in SEQ ID NO: 52.

[0383] In some embodiments, said binding agent comprises a kappa (K) light chain constant region.

[0384] In some embodiments, said binding agent comprises a lambda (X) light chain constant region.

[0385] In some embodiments, said first light chain constant region is a kappa (K) light chain constant region or a lambda (A) light chain constant region.

[0386] In some embodiments, said second light chain constant region is a kappa (K) light chain constant region or a lambda (A) light chain constant region.

[0387] In some embodiments,

[0388] (i) said first light chain constant region and said second light chain constant region are kappa (K) light chain constant regions,

[0389] (ii) said first light chain constant region and said second light chain constant region are lambda (A) light chain constant regions,

[0390] (iii) said first light chain constant region is a kappa (K) light chain constant region and said second light chain constant region is a lambda (A) light chain constant region, or

[0391] (iv) said first light chain constant region is a lambda (A) light chain constant region and said second light chain constant region is a kappa (K) light chain constant region. In some embodiments, the kappa (K) light chain comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 55.

[0392] In some embodiments, the lambda (A) light chain comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 56.

[0393] In some embodiments, the binding agent is of an isotype selected from the group consisting of IgGl, IgG2, IgG3, and IgG4. In one embodiment, the isotype is selected from the group consisting of human IgGl, human IgG2, human IgG3 and human IgG4.

[0394] In some embodiments, the binding agent is a full-length IgGl antibody.

[0395] In some embodiments, the binding agent is an antibody of the IgGlm(f) allotype.

[0396] The binding agent disclosed herein can in principle be an antibody of any isotype. The choice of isotype typically will be guided by the desired Fc-mediated effector functions, such as ADCC induction, or the requirement for an antibody devoid of Fc-mediated effector function ("inert" antibody). Exemplary isotypes are IgGl, IgG2, IgG3, and IgG4. Either of the human light chain constant regions, kappa or lambda, may be used. The effector function of the antibodies described herein may be changed by isotype switching to, e.g., an IgGl, IgG2, IgG3, IgG4, IgD, IgA, IgE, or IgM antibody for various therapeutic uses. In one embodiment, both heavy chains of an antibody described herein are of the IgGl isotype, for instance an IgGlK. Optionally, the heavy chain may be modified in the hinge and / or CH3 region as described elsewhere herein.

[0397] Preferably, each of the antigen-binding regions comprises a heavy chain variable region (VH) and a light chain variable region (VL), and wherein said variable regions each comprise three CDR sequences, CDR1, CDR2 and CDR3, respectively, and four framework sequences, FR1, FR2, FR3 and FR4, respectively. Furthermore, preferably, the antibody comprises two heavy chain constant regions (CH), and two light chain constant regions (CL).

[0398] In one embodiment, the binding agent is a full-length antibody, such as a full-length IgGl antibody. For example, in one embodiment, the binding agent, e.g., a bispecific antibody, comprises two half-molecules each comprising an antigen-binding region.

[0399] Many different formats of bispecific antibodies are known in the art, and were reviewed by Kontermann; Drug Discov Today, 2015 Jul;20(7):838-47 and; MAbs, 2012 Mar-Apr;4(2): 182-97. All these formats are encompassed herein._A bispecific antibody according to the present disclosure is not limited to any particular bispecific format or method of producing it.

[0400] Examples of bispecific antibody molecules which may be used in the present disclosure comprise (i) a single antibody that has two arms comprising different antigen-binding regions; (ii) a single chain antibody that has specificity to two different epitopes, e.g., via two scFvs linked in tandem by an extra peptide linker; (iii) a dual- variable-domain antibody (DVD-Ig), where each light chain and heavy chain contains two variable domains in tandem through a short peptide linkage (Wu et al., Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-Ig™) Molecule, In: Antibody Engineering, Springer Berlin Heidelberg (2010)); (iv) a chemically-linked bispecific (FabQ2 fragment; (v) a Tandab, which is a fusion of two single chain diabodies resulting in a tetravalent bispecific antibody that has two binding sites for each of the target antigens; (vi) a flexibody, which is a combination of scFvs with a diabody resulting in a multivalent molecule; (vii) a so-called "dock and lock" molecule, based on the "dimerization and docking domain" in Protein Kinase A, which, when applied to Fabs, can yield a trivalent bispecific binding protein consisting of two identical Fab fragments linked to a different Fab fragment; (viii) a so-called Scorpion molecule, comprising, e.g., two scFvs fused to both termini of a human Fab- arm; and (ix) a diabody.

[0401] In one embodiment, the binding agent described herein is a diabody or a cross-body. In one embodiment, the binding agent is a bispecific antibody obtained via a controlled Fab-arm exchange (such as described in WO20U131746 (Genmab)).

[0402] Examples of different classes of binding agents include but are not limited to (i) IgG-like molecules with complementary CH3 domains to force heterodimerization; (ii) recombinant IgG-like dual targeting molecules, wherein the two sides of the molecule each contain the Fab fragment or part of the Fab fragment of at least two different antibodies; (iii) IgG fusion molecules, wherein full length IgG antibodies are fused to extra Fab fragment or parts of Fab fragment; (iv) Fc fusion molecules, wherein single chain Fv molecules or stabilized diabodies are fused to heavy-chain constant-domains, Fc regions or parts thereof; (v) Fab fusion molecules, wherein different Fab-fragments are fused together, fused to heavy-chain constant-domains, Fc regions or parts thereof; and (vi) ScFv- and diabody-based and heavy chain antibodies (e.g., domain antibodies, nanobodies) wherein different single chain Fv molecules or different diabodies or different heavy-chain antibodies (e.g. domain antibodies, nanobodies) are fused to each other or to another protein or carrier molecule fused to heavy-chain constant-domains, Fc regions or parts thereof.

[0403] Examples of IgG-like molecules with complementary CH3 domain molecules include but are not limited to the Triomab / Quadroma molecules (Trion Pharma / Fresenius Biotech; Roche, WO2011069104), the so-called Knobs- into-Holes molecules (Genentech, WO9850431), CrossMAbs (Roche, WO2011117329) and the electrostatically- matched molecules (Amgen, EP1870459 and W02009089004; Chugai, US201000155133; Oncomed, W02010129304), the LUZ-Y molecules (Genentech, Wranik et al. J. Biol. Chem. 2012, 287(52): 43331-9, doi: 10.1074 / jbc.M112.397869. Epub 2012 Nov 1), DIG-body and PIG-body molecules (Pharmabcine, WO2010134666, W02014081202), the Strand Exchange Engineered Domain body (SEEDbody) molecules (EMD Serono, W02007110205), the Biclonics molecules (Merus, WO2013157953), FcAAdp molecules (Regeneron, W0201015792), bispecific IgGl and IgG2 molecules (Pfizer / Rinat, WO11143545), Azymetric scaffold molecules (Zymeworks / Merck, WO2012058768), mAb-Fv molecules (Xencor, WO2011028952), bivalent bispecific antibodies (W02009080254) and the DuoBody® molecules (Genmab, WO2011131746).

[0404] Examples of recombinant IgG-like dual targeting molecules include but are not limited to Dual Targeting (DT)-Ig molecules (W02009058383), Two-in-one Antibody (Genentech; Bostrom, et al 2009. Science 323, 1610-1614.), Cross-linked Mabs (Karmanos Cancer Center), mAb2 (F-Star, W02008003116), Zybody molecules (Zyngenia; LaFleur et al. MAbs. 2013 Mar-Apr;5(2):208-18), approaches with common light chain (Crucell / Merus, 1157,262,028), KXBodies (Novlmmune, W02012023053) and CovX-body (CovX / Pfizer; Doppalapudi, V.R., et al 2007. Bioorg. Med. Chem. Lett. 17,501-506.). Examples of IgG fusion molecules include but are not limited to Dual Variable Domain (DVD)-Ig molecules (Abbott, US7,612,181), Dual domain double head antibodies (Unilever; Sanofi Aventis, W020100226923), IgG-like Bispecific molecules (ImClone / Eli Lilly, Lewis et al. Nat Biotechnol. 2014 Feb;32(2): 191-8), Ts2Ab (Medlmmune / AZ; Dimasi et al. J Mol Biol. 2009 Oct 30;393(3):672-92) and BsAb molecules (Zymogenetics, W020101U625), HERCULES molecules (Biogen Idee, US007951918), scFv fusion molecules (Novartis), scFv fusion molecules (Changzhou Adam Biotech Inc, CN 102250246) and TvAb molecules (Roche, WO2012025525, W02012025530).

[0405] Examples of Fc fusion molecules include but are not limited to ScFv / Fc Fusions (Pearce et al., Biochem Mol Biol Int. 1997 Sep;42(6): 1179-88), SCORPION molecules (Emergent BioSolutions / Trubion, Blankenship JW, et al. AACR 100th Annual meeting 2009 (Abstract # 5465); Zymogenetics / BMS, W02010111625), Dual Affinity Retargeting Technology (Fc-DART) molecules (MacroGenics, WO2008157379, W02010080538) and Dual(ScFv)2-Fab molecules (National Research Center for Antibody Medicine - China).

[0406] Examples of Fab fusion bispecific antibodies include but are not limited to F(ab)2 molecules (Medarex / AMGEN; Deo et al J Immunol. 1998 Feb 15;160(4):1677-86.), Dual-Action or Bis-Fab molecules (Genentech, Bostrom, et al 2009. Science 323, 1610-1614.), Dock-and-Lock (DNL) molecules (ImmunoMedics, W02003074569, W02005004809), Bivalent Bispecific molecules (Biotecnol, Schoonjans, J Immunol. 2000 Dec 15;165(12):7050-7.) and Fab-Fv molecules (UCB-Celltech, WO 2009040562 Al).

[0407] Examples of ScFv-, diabody-based and domain antibodies include but are not limited to Bispecific T Cell Engager (BITE) molecules (Micromet, W02005061547), Tandem Diabody molecules (TandAb) (Affimed) Le Gall et al., Protein Eng Des Sei. 2004 Apr;17(4):357-66.), Dual Affinity Retargeting Technology (DART) molecules (MacroGenics, WO2008157379, W02010080538), Single-chain Diabody molecules (Lawrence, FEBS Lett. 1998 Apr 3;425(3):479-84), TCR-like Antibodies (AIT, ReceptorLogics), Human Serum Albumin ScFv Fusion (Merrimack, W02010059315) and COMBODY molecules (Epigen Biotech, Zhu et al. Immunol Cell Biol. 2010 Aug;88(6):667- 75.), dual targeting nanobodies (Ablynx, Hmila et al., FASEB J. 2010) and dual targeting heavy chain only domain antibodies.

[0408] In one embodiment, the bispecific antibody of the disclosure comprises a first Fc sequence comprising a first CH3 region, and a second Fc sequence comprising a second CH3 region, wherein the sequences of the first and second CH3 regions are different and are such that the heterodimeric interaction between said first and second CH3 regions is stronger than each of the homodimeric interactions of said first and second CH3 regions. More details on these interactions and how they can be achieved are provided in WO2011131746 and W02013060867 (Genmab), which are hereby incorporated by reference.

[0409] As described further herein, a stable bispecific EpCAMxCD137 antibody can be obtained at high yield using a particular method on the basis of one homodimeric starting EpCAM antibody and one homodimeric starting CD137 antibody containing only a few, conservative, asymmetrical mutations in the CH3 regions. Asymmetrical mutations mean that the sequences of said first and second CH3 regions contain amino acid substitutions at non-identical positions.

[0410] In one embodiment, the bispecific antibody as defined in any of the embodiments disclosed herein, comprises a first CH3 region which has an amino acid substitution at a position selected from the group consisting of: 366, 368, 370, 399, 405, 407 and 409 in a human IgGl heavy chain, and a second CH3 region which has an amino acid substitution at a position selected from the group consisting of: 366, 368, 370, 399, 405, 407 and 409 in a human IgGl heavy chain, and wherein the first and second CH3 regions are not substituted in the same positions.

[0411] In one embodiment, the bispecific antibody as defined in any of the embodiments disclosed herein, comprises the sequences of said first and second CH3 regions containing asymmetrical mutations, i.e. mutations at different positions in the two CH3 regions, e.g. a mutation at position 405 in one of the CH3 regions and a mutation at position 409 in the other CH3 region. In one embodiment, the mutation at position 405 is F405L. In one embodiment, the mutation at position 409 is K409R.

[0412] In one embodiment, the bispecific antibody comprises a first and second heavy chain, wherein each of said first and second heavy chains comprises at least a hinge region, a CH2 and a CH3 region, wherein (i) the amino acid in the position corresponding to F405 in human IgGl heavy chain is L in said first heavy chain, and the amino acid in the position corresponding to K409 in human IgGl heavy chain is R in said second heavy chain, or (ii) the amino acid in the position corresponding to K409 in human IgGl heavy chain is R in said first heavy chain, and the amino acid in the position corresponding to F405 in human IgGl heavy chain is L in said second heavy chain.

[0413] Traditional methods such as the hybrid hybridoma and chemical conjugation methods (Marvin and Zhu (2005) Acta Pharmacol Sin 26:649) can be used in the preparation of the bispecific antibodies described herein. Co-expression in a host cell of two antibodies, consisting of different heavy and light chains, leads to a mixture of possible antibody products in addition to the desired bispecific antibody, which can then be isolated by, e.g., affinity chromatography or similar methods.

[0414] Strategies favoring the formation of a functional bispecific, product, upon co-expression of different antibody constructs can also be used, e.g., the method described by Lindhofer et al. (1995 J Immunol 155:219). Fusion of rat and mouse hybridomas producing different antibodies leads to a limited number of heterodimeric proteins because of preferential species-restricted heavy / light chain pairing. Another strategy to promote formation of heterodimers over homodimers is a "knob-into-hole" strategy in which a protuberance is introduced on a first heavy- chain polypeptide and a corresponding cavity in a second heavy-chain polypeptide, such that the protuberance can be positioned in the cavity at the interface of these two heavy chains so as to promote heterodimer formation and hinder homodimer formation. "Protuberances" are constructed by replacing small amino-acid side-chains from the interface of the first polypeptide with larger side chains. Compensatory "cavities" of identical or similar size to the protuberances are created in the interface of the second polypeptide by replacing large amino-acid side-chains with smaller ones (US patent 5,731,168). EP1870459 (Chugai) and W02009089004 (Amgen) describe other strategies for favoring heterodimer formation upon co-expression of different antibody domains in a host cell. In these methods, one or more residues that make up the CH3-CH3 interface in both CH3 domains are replaced with a charged amino acid such that homodimer formation is electrostatically unfavorable and heterodimerization is electrostatically favorable. W02007110205 (Merck) describe yet another strategy, wherein differences between IgA and IgG CH3 domains are exploited to promote heterodimerization.

[0415] Another in vitro method for producing bispecific antibodies has been described in WO2008119353 (Genmab), wherein a bispecific antibody is formed by "Fab-arm" or "half-molecule" exchange (swapping of a heavy chain and attached light chain) between two monospecific IgG4- or IgG4-like antibodies upon incubation under reducing conditions. The resulting product is a bispecific antibody having two Fab arms which may comprise different sequences. A preferred method for preparing bispecific EpCAMxCD137 antibodies of the present disclosure includes the methods described in WO2011131746 and W02013060867 (Genmab) comprising the following steps: a) providing a first antibody comprising an Fc region, said Fc region comprising a first CH3 region; b) providing a second antibody comprising a second Fc region, said Fc region comprising a second CH3 region, wherein the first antibody is a EpCAM antibody and the second antibody is a CD137 antibody, or vice versa; wherein the sequences of said first and second CH3 regions are different and are such that the heterodimeric interaction between said first and second CH3 regions is stronger than each of the homodimeric interactions of said first and second CH3 regions; c) incubating said first antibody together with said second antibody under reducing conditions; and d) obtaining said bispecific EpCAMxCD137 antibody.

[0416] Similarly, there is provided a method for producing an antibody according to the disclosure, comprising the steps of: a) culturing a host cell producing a first antibody comprising an antigen-binding region capable of binding to human EpCAM as defined herein and purifying said first antibody from the culture; b) culturing a host cell producing a second antibody comprising an antigen-binding region capable of binding to human CD137 as defined herein and purifying said second antibody from the culture; c) incubating said first antibody together with said second antibody under reducing conditions sufficient to allow the cysteines in the hinge region to undergo disulfide-bond isomerization, and d) obtaining said bispecific antibody.

[0417] In one embodiment of the disclosure, the said first antibody together with said second antibody are incubated under reducing conditions sufficient to allow the cysteines in the hinge region to undergo disulfide-bond isomerization, wherein the heterodimeric interaction between said first and second antibodies in the resulting heterodimeric antibody is such that no Fab-arm exchange occurs at 0.5 mM GSH after 24 hours at 37°C.

[0418] Without being limited to theory, in step c), the heavy-chain disulfide bonds in the hinge regions of the parent antibodies are reduced and the resulting cysteines are then able to form inter heavy-chain disulfide bonds with cysteine residues of another parent antibody molecule (originally with a different specificity). In one embodiment of this method, the reducing conditions in step c) comprise the addition of a reducing agent, e.g. a reducing agent selected from the group consisting of: 2-mercaptoethylamine (2-MEA), dithiothreitol (DTT), dithioerythritol (DTE), glutathione, tris(2-carboxyethyl)phosphine (TCEP), L-cysteine and beta-mercapto-ethanol, preferably a reducing agent selected from the group consisting of: 2-mercaptoethylamine, dithiothreitol and tris(2- carboxyethyl)phosphine. In a further embodiment, step c) comprises restoring the conditions to become non- reducing or less reducing, for example by removal of a reducing agent, e.g. by desalting.

[0419] As described above, in one embodiment, the sequences of the first and second CH3 regions of the homodimeric starting antibodies are different and are such that the heterodimeric interaction between said first and second CH3 regions is stronger than each of the homodimeric interactions of said first and second CH3 regions. More details on these interactions and how they can be achieved are provided in WO2011131746 and W02013060867 (Genmab), which are hereby incorporated by reference in their entirety.

[0420] In particular, a stable bispecific EpCAMxCD137 antibody can be obtained at high yield using the above method on the basis of two homodimeric starting antibodies which bind EpCAM and CD137, respectively, and contain only a few, asymmetrical mutations in the CH3 regions. Asymmetrical mutations mean that the sequences of said first and second CH3 regions contain amino acid substitutions at non-identical positions.

[0421] In some embodiments, the binding agent according to the present disclosure comprises, in addition to the antigen- binding regions, an Fc region consisting of the Fc sequences of the two heavy chains.

[0422] The first and second Fc sequences may each be of any isotype, including, but not limited to, IgGl, IgG2, IgG3 and IgG4, and may comprise one or more mutations or modifications. In one embodiment, each of the first and second Fc sequences is of the IgGl isotype or derived therefrom, optionally with one or more mutations or modifications. In one embodiment, each of the first and second Fc sequences is of the IgG4 isotype or derived therefrom, optionally with one or more mutations or modifications. In another embodiment, one of the Fc sequences is of the IgGl isotype and the other of the IgG4 isotype, or is derived from such respective isotypes, optionally with one or more mutations or modifications.

[0423] In one embodiment, one or both Fc sequences are effector-function-deficient. For example, the Fc sequence(s) may be of an IgGl isotype, or a non-IgGl isotype, e.g. IgG2, IgG3, or IgG4, which has been mutated such that the ability to mediate effector functions, such as ADCC, has been reduced or even eliminated.

[0424] The term "effector functions" as used herein includes any functions mediated by components of the immune system that result, for example, in the killing of diseased cells such as tumor cells, or in the inhibition of tumor growth and / or inhibition of tumor development, including inhibition of tumor dissemination and metastasis. Preferably, the effector functions in the context of the present disclosure are T cell mediated effector functions. Such functions comprise ADCC, ADCP or CDC.

[0425] Antibody-dependent cell-mediated cytotoxicity (ADCC)

[0426] Antibody-dependent cell-mediated cytotoxicity (ADCC) is the killing of an antibody-coated target cell by a cytotoxic effector cell through a nonphagocytic process, characterised by the release of the content of cytotoxic granules or by the expression of cell death-inducing molecules. ADCC is independent of the immune complement system that also lyses targets but does not require any other cell. ADCC is triggered through interaction of target-bound antibodies (belonging to IgG or IgA or IgE classes) with certain Fc receptors (FcRs), glycoproteins present on the effector cell surface that bind the Fc region of immunoglobulins (Ig). Effector cells that mediate ADCC include natural killer (NK) cells, monocytes, macrophages, neutrophils, eosinophils and dendritic cells. ADCC is a rapid effector mechanism whose efficacy is dependent on a number of parameters (density and stability of the antigen on the surface of the target cell; antibody affinity and FcR-binding affinity). ADCC involving human IgGl, the most used IgG subclass for therapeutic antibodies, is highly dependent on the glycosylation profile of its Fc portion and on the polymorphism of Fey receptors.

[0427] Antibody-dependent cellular phagocytosis (ADCP)

[0428] ADCP is one crucial mechanism of action of many antibody therapies. It is defined as a highly regulated process by which antibodies eliminate bound targets via connecting its Fc domain to specific receptors on phagocytic cells, and eliciting phagocytosis. Unlike ADCC, ADCP can be mediated by monocytes, macrophages, neutrophils, and dendritic cells, through FcyRIIa, FcyRI, and FcyRIIIa, of which FcyRIIa (CD32a) on macrophages represent the predominant pathway. Complement-dependent cytotoxicity (CDC)

[0429] CDC is another cell-killing method that can be directed by antibodies. IgM is the most effective isotype for complement activation. IgGl and IgG3 are also both very effective at directing CDC via the classical complement- activation pathway. Preferably, in this cascade, the formation of antigen-antibody complexes results in the uncloaking of multiple Clq binding sites in close proximity on the CH2 domains of participating antibody molecules such as IgG molecules (Clq is one of three subcomponents of complement Cl). Preferably these uncloaked Clq binding sites convert the previously low-affinity Clq-IgG interaction to one of high avidity, which triggers a cascade of events involving a series of other complement proteins and leads to the proteolytic release of the effector-cell chemotactic / activating agents C3a and C5a. Preferably, the complement cascade ends in the formation of a membrane attack complex, which creates pores in the cell membrane that facilitate free passage of water and solutes into and out of the cell.

[0430] Antibodies described herein may comprise modifications in the Fc region. When an antibody comprises such modifications, it may become an inert, or non-activating, antibody. The term "inertness", "inert" or "non-activating" as used herein, refers to an Fc region which is at least not able to bind any Fey receptors, induce Fc-mediated cross-linking of FcRs, or induce FcR-mediated cross-linking of target antigens via two Fc regions of individual antibodies, or is not able to bind Clq. The inertness of an Fc region of a humanized or chimeric EpCAM or CD137 antibody is advantageously tested using the antibody in a monospecific format.

[0431] Several variants can be constructed to make the Fc region of an antibody inactive for interactions with Fey (gamma) receptors and Clq for therapeutic antibody development. Examples of such variants are described herein.

[0432] Thus, in one embodiment of the antibody described herein, said antibody comprises a first and a second heavy chain, wherein one or both heavy chains are modified so that the antibody induces Fc-mediated effector function to a lesser extent relative to an antibody which is identical, except for comprising non-modified first and second heavy chains. Said Fc-mediated effector function may be measured by determining, by binding to Fey receptors, by binding to Clq, or by induction of Fc-mediated cross-linking of FcRs.

[0433] In another such embodiment, the heavy and light chain constant sequences have been modified so that binding of Clq to said antibody is reduced compared to an unmodified antibody by at least 70%, at least 80%, at least 90%, at least 95%, at least 97%, or 100%, wherein Clq binding is determined by ELISA.

[0434] Thus, amino acids in the Fc region that play a dominant role in the interactions with Clq and the Fey receptors may be modified.

[0435] Examples of amino acid positions that may be modified, e.g. in an IgGl isotype antibody, include positions L234, L235, G236, D265 and P331. Combinations thereof, such as L234F / L235E / D265A, or L234F / L235E / G236R can cause a profound decrease in binding to human CD64, CD32, CD16 and Clq. Such modifications and their effect are described, e.g., in WO2022189667.

[0436] L234F and L235E amino acid substitutions can result in Fc regions with abrogated interactions with Fey receptors and Clq (Canfield et al., 1991, J. Exp. Med. (173): 1483-91; Duncan et al., 1988, Nature (332):738-40). Hence, in one embodiment, the amino acids in the positions corresponding to L234 and L235, may be F and E, respectively. A D265A or G236R amino acid substitution can decrease binding to all Fey receptors and prevent ADCC (Shields et al., 2001, J. Biol. Chem. (276):6591-604; Wilkinson et al., 2021, PloS one (16(12)) e0260954). Hence, in one embodiment, the amino acid in the position corresponding to D265 may be A or the amino acid in the position corresponding to G236 may be R.

[0437] In one embodiment of the disclosure, in both said first and second heavy chains the amino acids in the positions corresponding to positions L234 and L235 in a human IgGl heavy chain, are F and E, respectively.

[0438] In one embodiment, in one or both of said first and second heavy chains the amino acids in the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain, are F, E, and A, respectively.

[0439] In one embodiment, in one or both of said first and second heavy chains the amino acids in the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain, are F, E, and R, respectively.

[0440] The L234F-L235E-D265A non-activating mutations (also referred to herein as FEA or FEA format), have been shown to have an excellent safety profile and ability to strongly suppress Fc-mediated effector function. Nevertheless, it was observed that for IgGl antibodies that are potent inducers of complement-dependent cytotoxicity (CDC), harboring the FEA mutations can show some residual CDC. Furthermore, it was observed that recombinantly expressed antibodies with the FEA format may exhibit increased glycosylation heterogeneity as a result of additional processing of their N-glycans as compared with a wild-type IgGl Fc region and these antibodies were also shown to be more susceptible to aggregation induced by low pH conditions. Meanwhile, when the mutations L234F, L235E and G236R are combined (also referred to herein as FER, or FER format) in IgGl antibodies this resulted in an improved inert format capable of avoiding potential residual CDC activity, providing wild-type like glycosylation and having improved tolerance to low pH conditions. Thereby, the FER format is a highly advantageous non-activating antibody format that is well suitable for clinical development and clinical use. For bispecific antibodies, this FER inert format may also be combined in a heterodimeric format with respect to inert format substitutions, for example, a bispecific antibody may be composed of one chain carrying the inert format substitutions, whereas the other chain may comprise different inert format substitutions, e.g. FEA. Hence, the FER inert format is well suitable to be combined e.g. with existing candidate antibodies which have already undergone development for clinical use without the need to redesign and redo all the assays required, thereby allowing to quickly generate bispecific antibodies therewith utilizing technologies such as controlled Fab-arm exchange. Such modifications and their effect are described, e.g., in WO2022189667.

[0441] In a particularly preferred embodiment, in one of said first and second heavy chains the amino acids in the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain, are F, E, and A, respectively and in the other of said first and second heavy chains the amino acids in the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain, are F, E, and R, respectively.

[0442] In a further particularly preferred embodiment, the binding agent is a bispecific antibody comprising a first and second heavy chain, wherein the positions corresponding to positions L234 and L235 in a human IgGl heavy chain according to EU numbering of both the first heavy chain and the second heavy chain are F and E, respectively, and wherein (i) the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the first heavy chain is L, and the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the second heavy chain is R, or (ii) the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the first heavy chain is R, and the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the second heavy chain is L.

[0443] In a further particularly preferred embodiment, the binding agent is a bispecific antibody comprising a first and second heavy chain, wherein

[0444] (1) the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering of both the first heavy chain and the second heavy chain are F, E, and A, respectively,

[0445] (2) the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering of both the first heavy chain and the second heavy chain are F, E, and R, respectively, or

[0446] (3) the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering of one of the first heavy chain and the second heavy chain are F, E, and A, respectively, and the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering of the other of the first heavy chain and the second heavy chain are F, E, and R, respectively, and wherein (i) the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the first heavy chain is L, and the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the second heavy chain is R, or (ii) the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the first heavy chain is R, and the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the second heavy chain is L

[0447] Antibody variants having the combination of three amino acid substitutions L234F, L235E and D265A and in addition the K409R or the F405L mutation are herein termed with the suffix "FEAR" or "FEAL", respectively.

[0448] Antibody variants having the combination of three amino acid substitutions L234F, L235E and G236R and in addition the K409R or the F405L mutation are herein termed with the suffix "FERR" or "FERL", respectively.

[0449] In a preferred embodiment, the bispecific antibody described herein comprises:

[0450] (i) a half-molecule antibody derived from IgGl-EpCAM-FEAL, and a half-molecule antibody derived from IgGl- CD137-FEAR,

[0451] (ii) a half-molecule antibody derived from IgGl-EpCAM-FEAR, and a half-molecule antibody derived from IgGl-CD137-FEAL,

[0452] (iii) a half-molecule antibody derived from IgGl-EpCAM-FERL, and a half-molecule antibody derived from IgGl-CD137-FEAR, or

[0453] (iv) a half-molecule antibody derived from IgGl-EpCAM-FEAR, and a half-molecule antibody derived from IgGl-CD137-FERL.

[0454] In a further embodiment, the binding agents or antibodies described herein are linked or conjugated to one or more therapeutic moieties, such as a cytokine, an immune-suppressant, an immune-stimulatory molecule and / or a radioisotope. Such conjugates are referred to herein as "immunoconjugates" or "drug conjugates". Immunoconjugates which include one or more cytotoxins are referred to as "immunotoxins".

[0455] In one embodiment, the first and / or second Fc sequence is conjugated to a drug or a prodrug or contains an acceptor group for the same. Such acceptor group may e.g. be an unnatural amino acid.

[0456] In one embodiment of the binding agent binding to EpCAM and CD137, EpCAM is human EpCAM, in particular human EpCAM comprising the sequence set forth in SEQ ID NO: 59. In one embodiment of the binding agent binding to EpCAM and CD137, CD137 is human CD137, in particular human CD137 comprising the sequence set forth in SEQ ID NO: 62. In one embodiment, EpCAM is human EpCAM and CD137 is human CD137. In one embodiment, EpCAM is human EpCAM comprising the sequence set forth in SEQ ID NO: 59 and CD137 is human CD137 comprising the sequence set forth in SEQ ID NO: 62.

[0457] In one embodiment of the binding agent, a) the first binding region binding to human EpCAM comprises a heavy chain variable region (VH) comprising the CDR1, CDR2, and CDR3 sequences of SEQ ID NO: 1, and a light chain variable region (VL) comprising the CDR1, CDR2, and CDR3 sequences of SEQ ID NO: 5; and b) the second binding region binding to human CD137 comprises a heavy chain variable region (VH) comprising the CDR1, CDR2, and CDR3 sequences of SEQ ID NO: 11, and a light chain variable region (VL) comprising the CDR1, CDR2, and CDR3 sequences of SEQ ID NO: 15.

[0458] In one embodiment of the binding agent, a) the first binding region binding to human EpCAM comprises a heavy chain variable region (VH) comprising the CDR1, CDR2, and CDR3 sequences set forth in: SEQ ID NO: 2, 3, and 4, respectively, and a light chain variable region (VL) comprising the CDR1, CDR2, and CDR3 sequences set forth in: SEQ ID NO: 6, 7, and 8, respectively; and b) the second binding region binding to human CD137 comprises a heavy chain variable region (VH) comprising the CDR1, CDR2, and CDR3 sequences set forth in: SEQ ID NO: 12, 13, and 14, respectively, and a light chain variable region (VL) comprising the CDR1, CDR2, and CDR3 sequences set forth in: SEQ ID NO: 16, 17, and 18, respectively.

[0459] In one embodiment of the binding agent, a) the first binding region binding to human EpCAM comprises a heavy chain comprising an amino acid sequence having at least 90%, at least 95%, at least 97%, at least 99%, or 100% sequence identity to SEQ ID NO: 9 and a light chain comprising an amino acid sequence having at least 90%, at least 95%, at least 97%, at least 99%, or 100% sequence identity to SEQ ID NO: 10, and b) the second binding region binding to human CD137 comprises a heavy chain comprising an amino acid sequence having at least 90%, at least 95%, at least 97%, at least 99%, or 100% sequence identity to SEQ ID NO: 19 and a light chain comprising an amino acid sequence having at least 90%, at least 95%, at least 97%, at least 99%, or 100% sequence identity to SEQ ID NO: 20.

[0460] In one embodiment of the binding agent, a) the first binding region binding to human EpCAM comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 9 and a light chain comprising the amino acid sequence set forth in SEQ ID NO: 10, and b) the second binding region binding to human CD137 comprises a heavy chain comprising the amino add sequence set forth in SEQ ID NO: 19 and a light chain comprising the amino acid sequence set forth in SEQ ID NO: 20.

[0461] In one embodiment of the binding agent, a) the first binding region binding to human EpCAM comprises a heavy chain variable region (VH) comprising an amino acid sequence having at least 90%, at least 95%, at least 97%, at least 99%, or 100% sequence identity to SEQ ID NO: 1 and a light chain variable region (VL) region comprising an amino acid sequence having at least 90%, at least 95%, at least 97%, at least 99%, or 100% sequence identity to SEQ ID NO: 5, and b) the second binding region binding to human CD137 comprises a heavy chain variable region (VH) comprising an amino acid sequence having at least 90%, at least 95%, at least 97%, at least 99%, or 100% sequence identity to SEQ ID NO: 11 and a light chain variable region (VL) region and comprising an amino acid sequence having at least 90%, at least 95%, at least 97%, at least 99%, or 100% sequence identity to SEQ ID NO: 15.

[0462] In one embodiment of the binding agent, a) the first binding region binding to human EpCAM comprises a heavy chain variable region (VH) comprising the amino acid sequence set forth in SEQ ID NO: 1 and a light chain variable region (VL) region comprising the amino acid sequence set forth in SEQ ID NO: 5, and b) the second binding region binding to human CD137 comprises a heavy chain variable region (VH) comprising the amino acid sequence set forth in SEQ ID NO: 11 and a light chain variable region (VL) region comprising the amino acid sequence set forth in SEQ ID NO: 15.

[0463] The binding agent may in particular be an antibody, such as a multispecific antibody, e.g., a bispecific antibody. Also, the binding agent may be in the format of a full-length antibody or an antibody fragment.

[0464] It is further preferred that the binding agent is a human antibody or a humanized antibody.

[0465] Each variable region may comprise three complementarity determining regions (CDR1, CDR2, and CDR3) and four framework regions (FR1, FR2, FR3, and FR4).

[0466] The complementarity determining regions (CDRs) and the framework regions (FRs) may be arranged from amino- terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4.

[0467] In one embodiment, the binding agent is an antibody comprising a first binding arm and a second binding arm, wherein the first binding arm comprises i) a polypeptide comprising said first heavy chain variable region (VH) and said first heavy chain constant region (CH), and ii) a polypeptide comprising said first light chain variable region (VL) and said first light chain constant region (CL); and the second binding arm comprises iii) a polypeptide comprising said second heavy chain variable region (VH) and said second heavy chain constant region (CH), and iv) a polypeptide comprising said second light chain variable region (VL) and said second light chain constant region (CL).

[0468] In one embodiment, the binding agent comprises i) a first heavy chain and light chain comprising said antigen- binding region capable of binding to EpCAM, the first heavy chain comprising a first heavy chain constant region and the first light chain comprising a first light chain constant region; and ii) a second heavy chain and light chain comprising said antigen-binding region capable of binding CD137, the second heavy chain comprising a second heavy chain constant region and the second light chain comprising a second light chain constant region.

[0469] Each of the first and second heavy chain constant regions (CHs) may comprise a CH3 region, wherein the two CH3 regions comprise asymmetrical mutations. Asymmetrical mutations mean that the sequences of said first and second CH3 regions contain amino acid substitutions at non-identical positions. For example, one of said first and second CH3 regions contains a mutation at the position corresponding to position 405 in a human IgGl heavy chain according to EU numbering, and the other of said first and second CH3 regions contains a mutation at the position corresponding to position 409 in a human IgGl heavy chain according to EU numbering.

[0470] In said first heavy chain constant region (CH) at least one of the amino acids in a position corresponding to a position selected from the group consisting of T366, L368, K370, D399, F405, Y407, and K409 in a human IgGl heavy chain according to EU numbering may have been substituted, and in said second heavy chain constant region (CH) at least one of the amino acids in a position corresponding to a position selected from the group consisting of T366, L368, K370, D399, F405, Y407, and K409 in a human IgGl heavy chain according to EU numbering may have been substituted. In particular embodiments, the first and the second heavy chains are not substituted in the same positions (i.e., the first and the second heavy chains contain asymmetrical mutations).

[0471] In one embodiment of the binding agent, (i) the amino acid in the position corresponding to F405 in a human IgGl heavy chain according to EU numbering is L in said first heavy chain constant region (CH), and the amino acid in the position corresponding to K409 in a human IgGl heavy chain according to EU numbering is R in said second heavy chain constant region (CH), or (ii) the amino acid in the position corresponding to K409 in a human IgGl heavy chain according to EU numbering is R in said first heavy chain, and the amino acid in the position corresponding to F405 in a human IgGl heavy chain according to EU numbering is L in said second heavy chain.

[0472] In one embodiment, the binding agent induces Fc-mediated effector function to a lesser extent compared to another antibody comprising the same first and second antigen binding regions and two heavy chain constant regions (CHs) comprising human IgGl hinge, CH2 and CH3 regions.

[0473] In one particular embodiment of the binding agent, said first and second heavy chain constant regions (CHs) are modified so that the antibody induces Fc-mediated effector function to a lesser extent compared to an antibody which is identical except for comprising non-modified first and second heavy chain constant regions (CHs). In particular, each or both of said non-modified first and second heavy chain constant regions (CHs) may comprise, consists of or consist essentially of the amino acid sequence set forth in SEQ ID NO: 47.

[0474] The Fc-mediated effector function may be determined by measuring binding of the binding agent to Fey receptors, binding to Clq, or induction of Fc-mediated cross-linking of Fey receptors. In particular, the Fc-mediated effector function may be determined by measuring binding of the binding agent to Clq.

[0475] The first and second heavy chain constant regions of the binding agent may have been modified so that binding of Clq to said antibody is reduced compared to a wild-type antibody, preferably reduced by at least 70%, at least 80%, at least 90%, at least 95%, at least 97%, or 100%, wherein Clq binding is preferably determined by ELISA. In one embodiment of the binding agent, in at least one of said first and second heavy chain constant regions (CHs), one or more amino acids in the positions corresponding to positions L234, L235, G236, D265, N297, and P331 in a human IgGl heavy chain according to Ell numbering, are not L, L, G, D, N, and P, respectively.

[0476] In one embodiment of the binding agent, the positions corresponding to positions L234 and L235 in a human IgGl heavy chain according to EU numbering may be F and E, respectively, in said first and second heavy chains.

[0477] In particular, the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering may be F, E, and R, respectively, in said first and / or second heavy chain constant regions (HCs) and / or the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering may be F, E, and A, respectively, in said first and / or second heavy chain constant regions (HCs). In one embodiment, the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering may be F, E, and R, respectively, in one of said first and second heavy chain constant regions (HCs) and the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering may be F, E, and A, respectively, in the other of said first and second heavy chain constant regions (HCs). In one embodiment, the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering may be F, E, and R, respectively, in said first heavy chain constant region (HCs) and the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering may be F, E, and A, respectively, in said second heavy chain constant regions (HCs)

[0478] In one embodiment of the binding agent, the positions corresponding to positions L234 and L235 in a human IgGl heavy chain according to EU numbering of both the first and second heavy chain constant regions are F and E, respectively, wherein (i) the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is L, and the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is R, or (ii) the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is R, and the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is L.

[0479] In one embodiment of the binding agent, the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering of both the first and second heavy chain constant regions are F, E, and A, respectively, the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering of both the first and second heavy chain constant regions are F, E, and R, respectively, or the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering of one of the first and second heavy chain constant regions are F, E, and A, respectively, and the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering of the other of the first and second heavy chain constant regions are F, E, and R, respectively, wherein (i) the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is L, and the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is R, or (ii) the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the first heavy chain is R, and the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the second heavy chain is L. In one embodiment, the binding agent comprises a kappa (K) light chain constant region.

[0480] In one embodiment, the binding agent comprises a lambda (A) light chain constant region.

[0481] In one embodiment of the binding agent, the first light chain constant region is a kappa (K) light chain constant region or a lambda (A) light chain constant region.

[0482] In one embodiment of the binding agent, the second light chain constant region is a lambda (A) light chain constant region or a kappa (K) light chain constant region.

[0483] In one embodiment of the binding agent, the first light chain constant region is a kappa (K) light chain constant region and the second light chain constant region is a kappa (K) light chain constant region. In one embodiment of the binding agent, the first light chain constant region is a lambda (A) light chain constant region and the second light chain constant region is a lambda (A) light chain constant region. In one embodiment of the binding agent, the first light chain constant region is a kappa (K) light chain constant region and the second light chain constant region is a lambda (A) light chain constant region or the first light chain constant region is a lambda (A) light chain constant region and the second light chain constant region is a kappa (K) light chain constant region.

[0484] In one embodiment, the binding agent (in particular, antibody) is of an isotype selected from the group consisting of IgGl, IgG2, IgG3, and IgG4. In particular, the binding agent may be a full-length IgGl antibody. In preferred embodiments, the binding agent (in particular, antibody) is of the IgGlm(f) allotype.

[0485] PD-1 axis binding antagonist

[0486] "Immune checkpoint" refers to regulators of the immune system, and, in particular, co-stimulatory and inhibitory signals that regulate the amplitude and quality of T-cell activity. In certain embodiments, the immune checkpoint is an inhibitory signal. In certain embodiments, the inhibitory signal is the interaction between PD-1 and PD-L1 and / or PD-1 and PD-L2.

[0487] PD-1 is expressed predominantly on previously activated T cells in vivo, and binds to two ligands, PD-L1 and PD- L2. The term "PD-1" as used herein includes human PD-1 (hPD-1), variants, isoforms, and species homologs of hPD-1, and analogs having at least one common epitope with hPD-1. "Programmed Death Ligand-1 (PD-L1)" is one of two cell surface glycoprotein ligands for PD-1 (the other being PD-L2). The term "PD-L1" as used herein includes human PD-L1 (hPD-Ll), variants, isoforms, and species homologs of hPD-Ll, and analogs having at least one common epitope with hPD-Ll. The term "PD-L2" as used herein includes human PD-L2 (hPD-L2), variants, isoforms, and species homologs of hPD-L2, and analogs having at least one common epitope with hPD-L2. The interaction between PD-1 and its ligands results in a decrease in tumor infiltrating lymphocytes, a decrease in T-cell receptor mediated proliferation, and immune evasion by the cancerous cells. Immune suppression can be reversed by inhibiting the local interaction of PD-1 with PD-L1, and the effect is additive when the interaction of PD-1 with PD- L2 is blocked as well.

[0488] As used herein, the term "immune checkpoint modulator" or "checkpoint modulator" refers to a molecule or to a compound that modulates the function of one or more checkpoint proteins. Immune checkpoint modulators are typically able to modulate self-tolerance and / or the amplitude and / or the duration of the immune response. Preferably, the immune checkpoint modulator modulates the function of one or more human checkpoint proteins and is, thus, a "human checkpoint modulator". Specifically, the human checkpoint modulator is an immune checkpoint inhibitor.

[0489] As used herein, "immune checkpoint inhibitor" or "checkpoint inhibitor" refers to a molecule that totally or partially reduces, inhibits, interferes with or negatively modulates one or more checkpoint proteins or that totally or partially reduces, inhibits, interferes with or negatively modulates expression of one or more checkpoint proteins. In certain embodiments, the immune checkpoint inhibitor binds to one or more checkpoint proteins. In certain embodiments, the immune checkpoint inhibitor binds to one or more molecules regulating checkpoint proteins.

[0490] In certain embodiments, the immune checkpoint inhibitor prevents inhibitory signals associated with the immune checkpoint. In certain embodiments, the immune checkpoint inhibitor is an antibody, or fragment thereof that disrupts inhibitory signaling associated with the immune checkpoint. In certain embodiments, the immune checkpoint inhibitor is a small molecule inhibitor that disrupts inhibitory signaling. In certain embodiments, the immune checkpoint inhibitor is a peptide-based inhibitor that disrupts inhibitory signaling.

[0491] In certain embodiments, the immune checkpoint inhibitor is an antibody, fragment thereof, or antibody mimic, that prevents the interaction between checkpoint blocker proteins.

[0492] In some embodiments, inhibiting or blocking of inhibitory immune checkpoint signaling, as described herein, results in preventing or reversing immune-suppression and establishment or enhancement of T-cell immunity. In some embodiments, inhibition of immune checkpoint signaling, as described herein, reduces or inhibits dysfunction of the immune system. In some embodiments, inhibition of immune checkpoint signaling, as described herein, renders dysfunctional immune cells less dysfunctional. In some embodiments, inhibition of Immune checkpoint signaling, as described herein, renders a dysfunctional T cell less dysfunctional.

[0493] In certain embodiments, the inhibitory immunoregulator (immune checkpoint blocker) is a component of the PD- 1 / PD-L1 / PD-L2 signaling pathway.

[0494] In certain embodiments, the inhibitory immunoregulator (immune checkpoint blocker) is a PD(L)-1 axis binding antagonist.

[0495] The term "PD-1 axis binding antagonist" refers to a molecule that inhibits the interaction of a PD-1 axis binding partner with either one or more of its binding partners, such as PD-1, PD-L1 or PD-L2, so as to remove T-cell dysfunction resulting from signaling on the PD-1 signaling axis - with a result being to restore or enhance T-cell function (e.g., proliferation, cytokine production, target cell killing). As used herein, a PD-1 axis binding antagonist includes a PD-1 binding antagonist, a PD-L1 binding antagonist, and a PD-L2 binding antagonist.

[0496] In some embodiments, the PD-1 axis binding antagonist is a molecule that inhibits the binding of PD-1 to one or more of its binding partners. In a specific example, the PD-1 axis binding antagonist inhibits the binding of PD-1 to PD-L1 and / or PD-L2. For example, PD-1 axis binding antagonists include anti-PD-1, anti-PD-Ll, and anti-PD-L2 antibodies, antigen binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides and other molecules that decrease, block, inhibit, abrogate or interfere with signal transduction resulting from the interaction of PD-1 with PD-L1 and / or PD-L2. In some embodiments, a PD-1 axis binding antagonist reduces the negative co- stimulatory signal mediated by or through cell surface proteins expressed on T lymphocytes mediated signaling through PD-1 so as render a dysfunctional T-cell less dysfunctional (e.g., enhancing effector responses to antigen recognition). In some embodiments, the PD-1 axis binding antagonist is an anti-PD-1 antibody. Specific examples of PD-1 axis binding antagonists are provided, infra.

[0497] The term "PD-1 binding antagonist" refers to a molecule that decreases, blocks, inhibits, abrogates or interferes with signal transduction resulting from the interaction of PD-1 with one or more of its binding partners, such as PD- L1 and PD-L2. In some embodiments, the PD-1 binding antagonist is a molecule that inhibits the binding of PD-1 to one or more of its binding partners. In a specific example, the PD-1 binding antagonist inhibits the binding of PD-1 to PD-L1 and / or PD-L2. For example, PD-1 binding antagonists include anti-PD-1 antibodies, antigen binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides and other molecules that decrease, block, inhibit, abrogate or interfere with signal transduction resulting from the interaction of PD-1 with PD-L1 and / or PD- L2. In some embodiments, a PD-1 binding antagonist reduces the negative co-stimulatory signal mediated by or through cell surface proteins expressed on T lymphocytes mediated signaling through PD-1 so as render a dysfunctional T-cell less dysfunctional (e.g., enhancing effector responses to antigen recognition). In some embodiments, the PD-1 binding antagonist is an anti-PD-1 antibody. Specific examples of PD-1 binding antagonists are provided, infra.

[0498] The term "PD-L1 binding antagonist" refers to a molecule that decreases, blocks, inhibits, abrogates or interferes with signal transduction resulting from the interaction of PD-L1 with either one or more of its binding partners, such as PD-1, B7-1. In some embodiments, a PD-L1 binding antagonist is a molecule that inhibits the binding of PD-L1 to its binding partners. In a specific aspect, the PD-L1 binding antagonist inhibits binding of PD-L1 to PD-1 and / or B7-1. In some embodiments, the PD-L1 binding antagonists include anti-PD-Ll antibodies, antigen binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides and other molecules that decrease, block, inhibit, abrogate or interfere with signal transduction resulting from the interaction of PD-L1 with one or more of its binding partners, such as PD-1, B7-1. In some embodiments, a PD-L1 binding antagonist reduces the negative co-stimulatory signal mediated by or through cell surface proteins expressed on T lymphocytes mediated signaling through PD-L1 so as to render a dysfunctional T-cell less dysfunctional (e.g., enhancing effector responses to antigen recognition). In some embodiments, a PD-L1 binding antagonist is an anti-PD-Ll antibody. Specific examples of PD-L1 binding antagonists are provided, infra.

[0499] The term "PD-L2 binding antagonist" refers to a molecule that decreases, blocks, inhibits, abrogates or interferes with signal transduction resulting from the interaction of PD-L2 with either one or more of its binding partners, such as PD-1. In some embodiments, a PD-L2 binding antagonist is a molecule that inhibits the binding of PD-L2 to one or more of its binding partners. In a specific aspect, the PD-L2 binding antagonist inhibits binding of PD-L2 to PD-1. In some embodiments, the PD-L2 antagonists include anti-PD-L2 antibodies, antigen binding fragments thereof, immunoadhesins, fusion proteins, oligopeptides and other molecules that decrease, block, inhibit, abrogate or interfere with signal transduction resulting from the interaction of PD-L2 with either one or more of its binding partners, such as PD-1. In some embodiments, a PD-L2 binding antagonist reduces the negative co-stimulatory signal mediated by or through cell surface proteins expressed on T lymphocytes mediated signaling through PD-L2 so as render a dysfunctional T-cell less dysfunctional (e.g., enhancing effector responses to antigen recognition). In some embodiments, a PD-L2 binding antagonist is an immunoadhesin.

[0500] In some embodiments, a PD-1 axis binding antagonist includes a PD-1 binding antagonist, a PD-L1 binding antagonist and a PD-L2 binding antagonist. Alternative names for "PD-1" include CD279 and SLEB2. Alternative names for "PD-L1" include B7-H1, B7-4, CD274, and B7-H. Alternative names for"PD-L2" include B7-DC, Btdc, and CD273. In some embodiments, PD-1, PD-L1, and PD-L2 are human PD-1, PD-L1 and PD-L2.

[0501] In some embodiments, the PD-1 binding antagonist is a molecule that inhibits the binding of PD-1 to its ligand binding partner(s). In a specific embodiment, the PD-1 ligand binding partners are PD-L1 and / or PD-L2.

[0502] In some embodiments, the PD-L1 binding antagonist is a molecule that inhibits the binding of PD-L1 to its binding partner(s). In a specific aspect, PD-L1 binding partner(s) are PD-1 and / or B7-1.

[0503] In some embodiments, the PD-L2 binding antagonist is a molecule that inhibits the binding of PD-L2 to its binding partner(s). In a specific aspect, a PD-L2 binding partner is PD-1.

[0504] The antagonist may be an antibody, an antigen binding fragment thereof, an immunoadhesin, a fusion protein, or oligopeptide.

[0505] In some embodiments, the PD-1 axis binding antagonist is an anti-PD-1 antibody (e.g., a human antibody, a humanized antibody, or a chimeric antibody).

[0506] Exemplary PD-1 axis binding antagonists include, without limitation, anti-PD-1 antibodies such as BGB-A317 (BeiGene; see US 8,735,553, WO 2015 / 35606 and US 2015 / 0079109), cemiplimab (Regeneron; see WO 2015 / 112800) and lambrolizumab (e.g., disclosed as hPD109A and its humanized derivatives h409Al, h409A16 and h409A17 in WO2008 / 156712), AB137132 (Abeam), EH12.2H7 and RMP1-14 (#BE0146; Bioxcell Lifesciences Pvt. LTD.), MIH4 (Affymetrix eBioscience), nivolumab (OPDIVO, BMS-936558; Bristol Myers Squibb; see WO 2006 / 121168), pembrolizumab (KEYTRUDA; MK-3475; Merck; see WO 2008 / 156712), pidilizumab (CT-011; CureTech; see Hardy et al., 1994, Cancer Res., 54(22):5793-6 and WO 2009 / 101611), PDR001 (Novartis; see WO 2015 / 112900), MEDI0680 (AMP-514; AstraZeneca; see WO 2012 / 145493), TSR-042 (see WO 2014 / 179664), REGN-2810 (H4H7798N; cf. US 2015 / 0203579), JS001 (TAIZHOU JUNSHI PHARMA; see Si-Yang Liu et al., 2007, J. Hematol. Oncol. 70: 136), AMP-224 (GSK-2661380; cf. Li et al., 2016, Int J Mol Sci 17(7): 1151 and WO 2010 / 027827 and WO 2011 / 066342), PF-06801591 (Pfizer), BGB-A317 (BeiGene; see WO 2015 / 35606 and US 2015 / 0079109), BI 754091, SHR-1210 (see WO2015 / 085847), and antibodies 17D8, 2D3, 4H1, 4A11, 7D3, and 5F4 as described in WO 2006 / 121168, INCSHR1210 (Jiangsu Hengrui Medicine; also known as SHR-1210; see WO 2015 / 085847), TSR-042 (Tesaro Biopharmaceutical; also known as ANB011; see W02014 / 179664), GLS-010 (Wuxi / Harbin Gloria Pharmaceuticals; also known as WBP3055; see Si-Yang et al., 2017, J. Hematol. Oncol. 70: 136), STI-1110 (Sorrento Therapeutics; see WO 2014 / 194302), AGEN2034 (Agenus; see WO 2017 / 040790), MGA012 (Macrogenics; see WO 2017 / 19846), IBI308 (Innovent; see WO 2017 / 024465, WO 2017 / 025016, WO 2017 / 132825, and WO 2017 / 133540), anti-PD-1 antibodies as described, e.g., in US 7,488,802, US 8,008,449, US 8,168,757, WO 03 / 042402, WO 2010 / 089411 (further disclosing anti-PD-Ll antibodies), WO 2010 / 036959, WO 2011 / 159877 (further disclosing antibodies against TIM-3), WO 2011 / 082400, WO 2011 / 161699, WO 2009 / 014708, WO 03 / 099196, WO 2009 / 114335, WO 2012 / 145493 (further disclosing antibodies against PD-L1), WO 2015 / 035606, WO 2014 / 055648 (further disclosing anti-KIR antibodies), US 2018 / 0185482 (further disclosing anti- PD-Ll and anti-TIGIT antibodies), US 8,008,449, US 8,779,105, US 6,808,710, US 8,168,757, US 2016 / 0272708, and US 8,354,509.

[0507] In certain embodiments, the anti-PD-1 antibody comprises nivolumab (OPDIVO; BMS-936558), pembrolizumab (KEYTRUDA; MK-3475), cemiplimab (LIBTAYO, REGN2810), pidilizumab (CT-011), spartalizumab (PDR001), MEDI0680 (AMP-514), dostarlimab (TSR-042), cetrelimab (JNJ 63723283), toripalimab (JSOO1), AMP-224 (GSK- 2661380), PF-06801591, tislelizumab (BGB-A317), ABBV-181, BI 754091, or SHR-1210.

[0508] In some embodiments, the anti-PD-1 antibody is nivolumab (CAS Registry Number: 946414-94-4). Nivolumab (Bristol-Myers Squibb / Ono), also known as MDX-1106-04, MDX-1106, ONO-4538, BMS-936558, and OPDIVO®, is an anti-PD-1 antibody described in W02006 / 121168. In some embodiments, the anti-PD-1 antibody comprises a heavy chain and a light chain sequence depicted in SEQ ID NOS: 119 and 120, respectively.

[0509] In some embodiments, the anti-PD-1 antibody comprises the six CDR sequences from SEQ ID NO: 119 and SEQ ID NO: 120 (e.g., the three heavy chain CDRs from SEQ ID NO: 119 and the three light chain CDRs from SEQ ID NO: 120). In some embodiments, the anti-PD-1 antibody comprises the heavy chain variable domain from SEQ ID NO: 119 and the light chain variable domain from SEQ ID NO: 120. In some embodiments, the anti-PD-1 antibody comprises: a heavy chain variable region (VH) comprising an amino acid sequence of SEQ ID NO: 129, and (b) a light chain variable region (VL) comprising an amino acid sequence of SEQ ID NO: 130.

[0510] In some embodiments, the anti-PD-1 antibody comprises: (a) a heavy chain variable region (VH) that comprises a CDR-1 comprising an amino acid sequence of GITFSNSG (SEQ ID NO: 131), a CDR-2 comprising an amino acid sequence of IWYDGSKR (SEQ ID NO: 132), and a CDR-3 comprising an amino acid ATNDDY (SEQ ID NO: 133), and (b) a light chain variable region (VL) that comprises a CDR-1 comprising an amino acid sequence of QSVSSY (SEQ ID NO: 134), a CDR-2 comprising an amino acid sequence of DAS (SEQ ID NO: 135), and a CDR-3 comprising an amino acid sequence of QQSSNWPRT (SEQ ID NO: 136).

[0511] In a certain embodiment, the anti-PD-1 antibody is nivolumab which may be administered at a dose of 240 mg intravenously. Nivolumab may be given intravenously according to institutional guidelines, published guidelines and the respective product prescribing information, and dosed according to this protocol.

[0512] In some embodiments, the anti-PD-1 antibody is pembrolizumab (CAS Registry Number: 1374853-91-4). Pembrolizumab (Merck), also known as MK-3475, Merck 3475, lambrolizumab, KEYTRUDA®, and SCH-900475, is an anti-PD-1 antibody described in W02009 / 114335. In some embodiments, the anti-PD-1 antibody comprises a heavy chain and a light chain sequence depicted in SEQ ID NOS: 117 and 118, respectively.

[0513] In some embodiments, the anti-PD-1 antibody comprises the six CDR sequences from SEQ ID NO: 117 and SEQ ID NO: 118(e.#., the three heavy chain CDRs from SEQ ID NO: 117 and the three light chain CDRs from SEQ ID NO: 118). In some embodiments, the anti-PD-1 antibody comprises the heavy chain variable domain from SEQ ID NO: 117 and the light chain variable domain from SEQ ID NO: 118. In some embodiments, the anti-PD-1 antibody comprises: a heavy chain variable region (VH) comprising an amino acid sequence of SEQ ID NO: 115, and (b) a light chain variable region (VL) comprising an amino acid sequence of SEQ ID NO: 116.

[0514] In some embodiments, the anti-PD-1 antibody comprises: (a) a heavy chain variable region (VH) that comprises a CDR-1 comprising an amino acid sequence of GYTFTNYY (SEQ ID NO: 109), a CDR-2 comprising an amino acid sequence of INPSNGGT (SEQ ID NO: 110), and a CDR-3 comprising an amino acid ARRDYRFDMGFDY (SEQ ID NO: 111), and (b) a light chain variable region (VL) that comprises a CDR-1 comprising an amino acid sequence of KGVSTSGYSY (SEQ ID NO: 112), a CDR-2 comprising an amino acid sequence of LAS (SEQ ID NO: 113), and a CDR-3 comprising an amino acid sequence of QHSRDLPLT (SEQ ID NO: 114). In a certain embodiment, the anti-PD-1 antibody is pembrolizumab which may be administered at a dose of 200 mg intravenously. Pembrolizumab may be given intravenously according to institutional guidelines, published guidelines and the respective product prescribing information, and dosed according to this protocol.

[0515] In some embodiments, the anti-PD-1 antibody is IgGl-PDl. In some embodiments, the anti-PD-1 antibody comprises a heavy chain and a light chain sequence depicted in SEQ ID NOS: 107 and 108, respectively.

[0516] In some embodiments, the anti-PD-1 antibody comprises the six CDR sequences from SEQ ID NO: 107 and SEQ ID NO: 108 (e.g., the three heavy chain CDRs from SEQ ID NO: 107 and the three light chain CDRs from SEQ ID NO: 108). In some embodiments, the anti-PD-1 antibody comprises the heavy chain variable domain from SEQ ID NO: 107 and the light chain variable domain from SEQ ID NO: 108. In some embodiments, the anti-PD-1 antibody comprises: a heavy chain variable region (VH) comprising an amino acid sequence of SEQ ID NO: 105, and (b) a light chain variable region (VL) comprising an amino acid sequence of SEQ ID NO: 106.

[0517] In some embodiments, the anti-PD-1 antibody comprises: (a) a heavy chain variable region (VH) that comprises a CDR-1 comprising an amino acid sequence of GFSLYSYN (SEQ ID NO: 99), a CDR-2 comprising an amino acid sequence of ISGGTIG (SEQ ID NO: 100), and a CDR-3 comprising an amino acid ARAFYDDYDYNV (SEQ ID NO: 101), and (b) a light chain variable region (VL) that comprises a CDR-1 comprising an amino acid sequence of QSVYGNNQ (SEQ ID NO: 102), a CDR-2 comprising an amino acid sequence of QAS (SEQ ID NO: 103), and a CDR- 3 comprising an amino acid sequence of AGGYSSSSDTT (SEQ ID NO: 104).

[0518] In a certain embodiment, the anti-PD-1 antibody is IgGl-PDl which may be administered at a dose of 200 mg intravenously. IgGl-PDl may be given intravenously according to institutional guidelines, published guidelines and the respective product prescribing information, and dosed according to this protocol.

[0519] In certain embodiments, the anti-PD-1 antibody comprises cemiplimab.

[0520] In certain embodiments, the anti-PD-1 antibody comprises an antibody comprising a heavy chain and a light chain sequence depicted in SEQ ID NOS: 121 and 122, respectively.

[0521] In certain embodiments, the immune checkpoint inhibitor comprises an antibody comprising the six CDR sequences from SEQ ID NO: 121 and SEQ ID NO: 122 (e.g., the three heavy chain CDRs from SEQ ID NO: 121 and the three light chain CDRs from SEQ ID NO: 122). In certain embodiments, the immune checkpoint inhibitor comprises an antibody comprising the heavy chain variable domain from SEQ ID NO: 121 and the light chain variable domain from SEQ ID NO: 122.

[0522] In certain embodiments, the immune checkpoint inhibitor comprises an antibody comprising: (a) a heavy chain variable region (VH) that comprises a CDR-1 comprising the amino acid sequence FTFSNFG (SEQ ID NO: 137), a CDR-2 comprising the amino acid sequence ISGGGRDT (SEQ ID NO: 138), and a CDR-3 comprising the amino acid sequence VKWGNIYFDY (SEQ ID NO: 139), and (b) a light chain variable region (VL) that comprises a CDR-1 comprising the amino acid sequence LSINTF (SEQ ID NO: 140), a CDR-2 comprising the amino acid sequence AAS (SEQ ID NO: 141), and a CDR-3 comprising the amino acid sequence QQSSNTPFT (SEQ ID NO: 142). In some embodiments, the anti-PD-1 antibody is MEDI-0680 (AMP-514; AstraZeneca). MEDI-0680 is a humanized IgG4 anti-PD-1 antibody.

[0523] In some embodiments, the anti-PD-1 antibody is PDR001 (CAS Registry No. 1859072-53-9; Novartis). PDR001 is a humanized IgG4 anti-PDl antibody that blocks the binding of PD-L1 and PD-L2 to PD-1.

[0524] In some embodiments, the anti-PD-1 antibody is REGN2810 (Regeneron). REGN2810 is a human anti-PDl antibody also known as LIBTAYO® and cemiplimab-rwlc.

[0525] In some embodiments, the anti-PD-1 antibody is BGB-108 (BeiGene). In some embodiments, the anti-PD-1 antibody is BGB-A317 (BeiGene).

[0526] In some embodiments, the anti-PD-1 antibody is JS-001 (Shanghai Junshi). JS-001 is a humanized anti-PDl antibody.[052...

Claims

We Claim:

1. A method for treating or preventing a disease or disorder in a subject, said method comprising (i) administering to the subject a binding agent, which binding agent comprises a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137, and (ii) administering to the subject a PD-1 axis binding antagonist, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD-1 axis binding antagonist.

2. The method of claim 1, wherein the EpCAM is human EpCAM and / or wherein the CD137 is human CD137.

3. The method of claim 1 or 2, wherein said first antigen-binding region binding to EpCAM binds to EpCAM expressed on tumor cells.

4. The method of any one of claims 1 to 3, wherein said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 4.

5. The method of any one of claims 1 to 4, wherein said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 3.

6. The method of any one of claims 1 to 5, wherein said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a HCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 2.

7. The method of any one of claims 1 to 6, wherein said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively.

8. The method of any one of claims 1 to 7, wherein said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 8.

9. The method of any one of claims 1 to 8, wherein said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 7.

10. The method of any one of claims 1 to 9, wherein said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a LCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 6.

11. The method of any one of claims 1 to 10, wherein said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2 and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively.

12. The method of any one of claims 1 to 11, wherein said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively.

13. The method of any one of claims 1 to 12, wherein said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 1.

14. The method of any one of claims 1 to 13, wherein said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising the sequence as set forth in SEQ ID NO: 1.

15. The method of any one of claims 1 to 14, wherein said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 5.

16. The method of any one of claims 1 to 15, wherein said first antigen-binding region binding to EpCAM comprises a light chain variable region (VL) comprising the sequence as set forth in SEQ ID NO: 5.

17. The method of any one of claims 1 to 16, wherein said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 1 and the VL comprises the sequence as set forth in SEQ ID NO: 5.

18. The method of any one of claims 1 to 17, wherein said first antigen-binding region binding to EpCAM comprises heavy and light chain variable regions of an antibody which competes for EpCAM binding with and / or has the specificity for EpCAM of an antibody comprising a heavy chain variable region (VH) and / or a light chain variable region (VL) as set forth in any one of claims 5 to 18.

19. The method of any one of claims 1 to 18, wherein said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 14.

20. The method of any one of claims 1 to 19, wherein said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 13.

21. The method of any one of claims 1 to 20, wherein said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a HCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 12.

22. The method of any one of claims 1 to 21, wherein said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences, wherein theHCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively.

23. The method of any one of claims 1 to 22, wherein said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a LCDR3 sequence comprising the sequence as set forth in SEQ ID NO: 18.

24. The method of any one of claims 1 to 23, wherein said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a LCDR2 sequence comprising the sequence as set forth in SEQ ID NO: 17.

25. The method of any one of claims 1 to 24, wherein said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a LCDR1 sequence comprising the sequence as set forth in SEQ ID NO: 16.

26. The method of any one of claims 1 to 25, wherein said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

27. The method of any one of claims 1 to 26, wherein said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

28. The method of any one of claims 1 to 27, wherein said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 11.

29. The method of any one of claims 1 to 28, wherein said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising the sequence as set forth in SEQ ID NO: 11.

30. The method of any one of claims 1 to 29, wherein said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising a sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identity to the sequence as set forth in SEQ ID NO: 15.

31. The method of any one of claims 1 to 30, wherein said second antigen-binding region binding to CD137 comprises a light chain variable region (VL) comprising the sequence as set forth in SEQ ID NO: 15.

32. The method of any one of claims 1 to 31, wherein said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the sequence as set forth in SEQ ID NO: 11 and the VL comprises the sequence as set forth in SEQ ID NO: 15.

33. The method of any one of claims 1 to 32, wherein said second antigen-binding region binding to CD137 comprises heavy and light chain variable regions of an antibody which competes for CD137 binding with and / or has the specificity for CD137 of an antibody comprising a heavy chain variable region and / or a light chain variable region as set forth in any one of claims 20 to 33.

34. The method of any one of claims 1 to 33, wherein a) said first antigen-binding region binding to EpCAM comprises a heavy chain variable region (VH) comprising HCDR1, HCDR.2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively; and b) said second antigen-binding region binding to CD137 comprises a heavy chain variable region (VH) comprising HCDR1, HCDR2, and HCDR3 sequences and a light chain variable region (VL) comprising LCDR1, LCDR2, and LCDR3 sequences, wherein the HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the LCDR1, LCDR2, and LCDR3 sequences comprises the sequences as set forth in SEQ ID NO: 16, 17 and 18, respectively.

35. The method of any one of claims 1 to 34, wherein the binding agent is in the format of a full-length antibody or an antibody fragment.

36. The method of any one of claims 1 to 35, wherein the binding agent comprises an Fc region.

37. The method of any one of claims 1 to 36, wherein the binding agent is a multispecific such as a bispecific binding agent.

38. The method of any one of claims 1 to 37, wherein the binding agent is a bispecific bivalent binding agent.

39. The method of any one of claims 1 to 38, wherein the binding agent does not comprise an antigen-binding region binding to EpCAM other than said first antigen-binding region binding to EpCAM and does not comprise an antigen-binding region binding to CD137 other than said second antigen-binding region binding to CD137.

40. The method of any one of claims 1 to 39, wherein the binding agent is an antibody comprising a first binding arm comprising said first antigen-binding region binding to EpCAM and a second binding arm comprising said second antigen-binding region binding to CD137, wherein the first binding arm comprises i) a polypeptide comprising a first heavy chain variable region (VH) and a first heavy chain constant region (CH), and ii) a polypeptide comprising a first light chain variable region (VL) and a first light chain constant region (CL); and the second binding arm comprises iii) a polypeptide comprising a second heavy chain variable region (VH) and a second heavy chain constant region (CH), and iv) a polypeptide comprising a second light chain variable region (VL) and a second light chain constant region (CL).

41. The method of claim 40, wherein the first VH comprises first HCDR1, HCDR2, and HCDR3 sequences and the first VL comprises first LCDR1, LCDR2, and LCDR3 sequences, wherein the first HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively, and the first LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively; and the second VH comprises second HCDR1, HCDR2, and HCDR3 sequences and the second VL comprises second LCDR1, LCDR2, and LCDR3 sequences, wherein the second HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the second LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 16, 17, and 18, respectively.

42. The method of claim 40 or 41, wherein the first VH comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 1 and the first VL comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 5; and the second VH comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 11 and the second VL comprises an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence as set forth in SEQ ID NO: 15.

43. The method of any one of claims 40 to 42, wherein the first VH comprises the amino acid sequence as set forth in SEQ ID NO: 1 and the first VL comprises the amino acid sequence as set forth in SEQ ID NO: 5; and the second VH comprises the amino acid sequence as set forth in SEQ ID NO: 11 and the second VL comprises the amino acid sequence as set forth in SEQ ID NO: 15.

44. The method of any one of claims 40 to 43, wherein each of the first and second heavy chain constant regions (CHs) comprises one or more of a constant heavy chain 1 (CHI) region, a hinge region, a constant heavy chain 2 (CH2) region and a constant heavy chain 3 (CH3) region, preferably at least a hinge region, a CH2 region and a CH3 region.

45. The method of any one of claims 40 to 44, wherein each of the first and second heavy chain constant regions (CHs) comprises a CH3 region and wherein the two CH3 regions comprise asymmetrical mutations.

46. The method of any one of claims 40 to 45, wherein (i) the amino acid in the position corresponding to F405 in a human IgGl heavy chain according to EU numbering is L in said first heavy chain constant region (CH), and the amino acid in the position corresponding to K409 in a human IgGl heavy chain according to EU numbering is R in said second heavy chain constant region (CH), or (ii) the amino acid in the position corresponding to K409 in a human IgGl heavy chain according to EU numbering is R in said first heavy chain constant region (CH), and the amino acid in the position corresponding to F405 in a human IgGl heavy chain according to EU numbering is L in said second heavy chain constant region (CH).

47. The method of any one of claims 40 to 46, wherein said binding agent induces Fc-mediated effector function to a lesser extent compared to another antibody comprising the same first and second antigen binding regions and two heavy chain constant regions (CHs) comprising human IgGl hinge, CH2 and CH3 regions.

48. The method of any one of claims 40 to 47, wherein said first and second heavy chain constant regions (CHs) are modified so that the antibody induces Fc-mediated effector function to a lesser extent compared to an antibody which is identical except for comprising non-modified first and second heavy chain constant regions (CHs).

49. The method of any one of claims 40 to 48, wherein in at least one of said first and second heavy chain constant regions (CHs), one or more amino acids in the positions corresponding to positions L234, L235, D265, N297, P331, and G236 in a human IgGl heavy chain according to EU numbering, are not L, L, D, N, P, and G, respectively.

50. The method of claim 49, wherein the positions corresponding to positions L234 and L235 in a human IgGl heavy chain according to EU numbering are F and E, respectively, in said first and second heavy chains.

51. The method of claim 49 or 50, wherein the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A, respectively, in said first and / or second heavy chain constant regions (HCs) and / or the positions corresponding to positions 1234, L235, and G236 in a human IgGl heavy chain according to EU numbering are F, E, and R, respectively, in said first and / or second heavy chain constant regions (HCs).

52. The method of any one of claims 49 to 51, wherein(i) the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A, respectively, in both said first and second heavy chain constant regions;(ii) the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering are F, E, and R, respectively, in both said first and second heavy chain constant regions; or(iii) the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A, respectively, in one of said first and second heavy chain constant regions and the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering are F, E, and R, respectively, in the other of said first and second heavy chain constant regions.

53. The method of any one of claims 49 to 52, wherein the positions corresponding to positions L234 and L235 in a human IgGl heavy chain according to EU numbering of both the first and second heavy chain constant regions are F and E, respectively, and wherein (i) the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is L, and the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is R, or (ii) the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is R, and the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is L.

54. The method of any one of claims 49 to 53, wherein the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A, respectively, in said second heavy chain constant region (HC) and the positions corresponding to positions L234, L235, and G236 in a humanIgGl heavy chain according to EU numbering are F, E, and R, respectively, in said first heavy chain constant region (HC), and wherein (i) the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is L, and the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is R, or (ii) the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is R, and the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is L.

55. The method of any one of claims 49 to 54, wherein the positions corresponding to positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A, respectively, in said second heavy chain constant region (HC) and the positions corresponding to positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering are F, E, and R, respectively, in said first heavy chain constant region (HC), and wherein the position corresponding to F405 in a human IgGl heavy chain according to EU numbering of the first heavy chain constant region is L, and the position corresponding to K409 in a human IgGl heavy chain according to EU numbering of the second heavy chain constant region is R.

56. The method of any one of claims 40 to 55, wherein a) the constant region of said first heavy chain comprises the amino acid sequence set forth in SEQ ID NO: 54; and b) the constant region of said second heavy chain comprises the amino acid sequence set forth in SEQ ID NO: 52.

57. The method of any one of claims 1 to 56, wherein the binding agent is an antibody comprising a first binding arm comprising said first antigen-binding region binding to EpCAM and a second binding arm comprising said second antigen-binding region binding to CD137, wherein the first binding arm comprisesI) a polypeptide comprising a first heavy chain variable region (VH) and a first heavy chain constant region (CH), and ii) a polypeptide comprising a first light chain variable region (VL) and a first light chain constant region (CL), wherein the first VH comprises first HCDR1, HCDR2, and HCDR3 sequences and the first VL comprises first LCDR1, LCDR2, and LCDR3 sequences, wherein the first HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 2, 3, and 4, respectively, and the first LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 6, 7, and 8, respectively; and the second binding arm comprises iii) a polypeptide comprising a second heavy chain variable region (VH) and a second heavy chain constant region (CH), and iv) a polypeptide comprising a second light chain variable region (VL) and a second light chain constant region (CL), wherein the second VH comprises second HCDR1, HCDR2, and HCDR3 sequences and the second VL comprises second LCDR1, LCDR2, and LCDR3 sequences, wherein the second HCDR1, HCDR2, and HCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 12, 13, and 14, respectively, and the second LCDR1, LCDR2, and LCDR3 sequences comprise the sequences as set forth in SEQ ID NO: 16, 17, and 18, respectively;wherein positions L234, L235, and G236 in a human IgGl heavy chain according to EU numbering are F, E, and R, respectively, in the first CH and positions L234, L235, and D265 in a human IgGl heavy chain according to EU numbering are F, E, and A in the second CH; and wherein the amino acid in the position corresponding to F405 in a human IgGl heavy chain according to EU numbering is L in the first CH and the amino acid in the position corresponding to K409 in a human IgGl heavy chain according to EU numbering is R in the second CH.

58. The method of any one of claims 1 to 57, wherein the binding agent comprises i) a first heavy chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 9, ii) a first light chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 10, iii) a second heavy chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 19; and iv) a second light chain comprising an amino acid sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99% identity to the amino acid sequence set forth in SEQ ID NO: 20.

59. The method of any one of claims 1 to 58, wherein the binding agent comprisesI) a first heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 9, ii) a first light chain comprising the amino acid sequence set forth in SEQ ID NO: 10, iii) a second heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 19; and iv) a second light chain comprising the amino acid sequence set forth in SEQ ID NO: 20.

60. The method of any one of claims 1 to 59, wherein the PD-1 axis binding antagonist comprises a PD-1 binding antagonist.

61. The method of claim 60, wherein the PD-1 binding antagonist comprises an anti-PD-1 antibody.

62. The method of claim 61, wherein the PD-1 antibody is selected from the group consisting of IgGl-PDl, nivolumab, cemiplimab, dostarlimab or pembrolizumab, optionally wherein IgGl-PDl comprises (a) a heavy chain variable region (VH) that comprises a CDR-1 comprising an amino acid sequence of GFSLYSYN (SEQ ID NO:99), a CDR-2 comprising an amino acid sequence of ISGGTIG (SEQ ID NO:100), and a CDR-3 comprising an amino acid ARAFYDDYDYNV (SEQ ID NO: 101), and (b) a light chain variable region (VL) that comprises a CDR-1 comprising an amino acid sequence of QSVYGNNQ (SEQ ID NO: 102), a CDR-2 comprising an amino acid sequence of QAS (SEQ ID NO:103), and a CDR-3 comprising an amino acid sequence of AGGYSSSSDTT (SEQ ID NO:104).

63. The method of claim 61 or 62, wherein the PD-1 antibody is IgGl-PDl.

64. The method of any one of claims 1 to 59, wherein the PD-1 axis binding antagonist comprises a PD-L1 binding antagonist.

65. The method of claim 64, wherein the PD-L1 binding antagonist comprises an anti-PD-Ll antibody.

66. The method of claim 65, wherein the anti-PD-Ll antibody is selected from the group consisting of atezolizumab, avelumab or durvalumab.

67. The method of any one of claims 1 to 59, wherein the PD-1 axis binding antagonist comprises a PD-L2 binding antagonist.

68. The method of claim 67, wherein the PD-L2 binding antagonist comprises an anti-PD-L2 antibody.

69. The method of claim 68, wherein the anti-PD-L2 antibody is selected from the group consisting of OT17B10 and PDL2 / 1850.

70. The method of any one of claims 1 to 69, wherein the disease or disorder concerns unregulated cell growth.

71. The method of claim 70, wherein the unregulated cell growth is cancer or a tumor.

72. The method of claim 71, wherein the cancer or tumor is a solid tumor.

73. The method of claim 71 or 72, wherein the cancer, tumor, or solid tumor is selected from the group consisting of cholangiocarcinoma (bile duct cancer), gastric / gastroesophageal junction (GEJ) cancer, melanoma, ovarian cancer, lung cancer (e.g., non-small cell lung cancer (NSCLC)), colorectal cancer, head and neck cancer, gastric cancer, breast cancer, renal cancer, urothelial cancer, bladder cancer, esophageal cancer, pancreatic cancer, hepatic cancer, thymoma and thymic carcinoma, brain cancer, glioma, adrenocortical carcinoma, thyroid cancer, other skin cancers, sarcoma, multiple myeloma, leukemia, lymphoma, myelodysplastic syndromes, endometrial cancer, prostate cancer, penile cancer, cervical cancer, Hodgkin’s lymphoma, non-Hodgkin's lymphoma, Merkel cell carcinoma and mesothelioma.

74. The method of any one of claims 1 to 73, wherein the disease or disorder is cholangiocarcinoma (bile duct cancer) or gastric / gastroesophageal junction (GEJ).

75. The method of any one of claims 1 to 74, wherein the subject is a mammalian subject.

76. The method of claim 75, wherein the mammalian subject is a human subject.

77. The method of any one of claims 1 to 76, wherein the subject has not received prior treatment for the disease or disorder.

78. The method of any one of claims 1 to 76, wherein the subject has received prior treatment for the disease or disorder.

79. The method of claim 77, wherein the subject has not received prior treatment with a checkpoint inhibitor.

80. The method of any one of claims 1 to 79, wherein the method further comprises administering to the subject one or more additional therapeutic agents.

81. The method of claim 80, wherein the one or more additional therapeutic agents comprise one or more chemotherapeutic agents, such as platinum-based compounds (e.g., cisplatin, oxaliplatin, and carboplatin), taxane- based compounds (e.g., paclitaxel and nab-paclitaxel), nucleoside analogs (e.g., 5-fluorouracil and gemcitabine), and combinations thereof (e.g., cisplatin / carboplatin + 5-fluorouracil or nab-paclitaxel + gemcitabine).

82. The method of any one of claims 1 to 81, wherein administering the binding agent to the subject comprises administering one or more nucleic acids encoding the binding agent.

83. The method of any one of claims 1 to 82, wherein administering the PD-1 axis binding antagonist to the subject comprises administering one or more nucleic acids encoding the PD-1 axis binding antagonist.

84. The method of any one of claims 1 to 83, wherein administering the binding agent and the PD-1 axis binding antagonist to the subject comprises administering one or more nucleic acids encoding both the binding agent and the PD-1 axis binding antagonist.

85. The method of any one of claims 82 to 84, wherein at least one of the nucleic acids is RNA.

86. A polynucleotide or a set of polynucleotides encoding the binding agent and / or the PD-1 axis binding antagonist defined in of any one of claims 1 to 69.

87. A kit comprising, in one or more vials, (i) a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137, or one or more nucleic acids encoding the binding agent, (ii) a PD-1 axis binding antagonist or one or more nucleic acids encoding the PD-1 axis binding antagonist, and optionally (iii) one or more therapeutic agents.

88. The kit of claim 87, wherein the kit further comprises instructions for the use of the kit in treating or preventing a disease or disorder in a subject.

89. The kit of claim 87 or 88, wherein the binding agent or encoding nucleic acid and the PD-1 axis binding antagonist or encoding nucleic acid are in separate vials.

90. The kit of any one of claims 87 to 89 for use in a method of treating or preventing a disease or disorder in a subject, preferably wherein the disease or disorder is a tumor, solid tumor, or cancer, wherein said method comprises (i) administering to the subject the binding agent, and (ii) administering to the subject the PD-1 axis binding antagonist, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD-1 axis binding antagonist.

91. The kit of any one of claims 87 to 89 for use in a method of reducing or preventing progression of a tumor, solid tumor or cancer in a subject, wherein said method comprises (i) administering to the subject the binding agent, and (ii) administering to the subject the PD-1 axis binding antagonist, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD-1 axis binding antagonist.

92. The kit of any one of claims 87 to 89 or the kit for use of claim 90 or 91, wherein the binding agent is defined in any one of claims 1 to 59.

93. The kit of any one of claims 87 to 89 or the kit for use of any one of claims 90 to 92, wherein the PD-1 axis binding antagonist is defined in any one of claims 60 to 69.

94. The kit of any one of claims 87 to 89 or the kit for use of any one of claims 90 to 93, wherein the one or more therapeutic agents are defined in claim 81.

95. The kit of any one of claims 87 to 89 or the kit for use of any one of claims 90 to 94, wherein the tumor, solid tumor or cancer are defined in claim 73 or 74.

96. The kit of any one of claims 87 to 89 for use in the method of any one of claims 1 to 85.

97. A binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137 for use in a method for treating or preventing a disease or disorder in a subject, said method comprising administering the binding agent to the subject in combination with a PD-1 axis binding antagonist, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD-1 axis binding antagonist.

98. A PD-1 axis binding antagonist for use in a method for treating or preventing a disease or disorder in a subject, said method comprising administering the PD-1 axis binding antagonist to the subject in combination with a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137, wherein the administration of the PD-1 axis binding antagonist is prior to, simultaneously with, or after administration of the binding agent.

99. A binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137 and a PD-1 axis binding antagonist for use in a method for treating or preventing a disease or disorder in a subject, said method comprising administering the binding agent and the PD-1 axis binding antagonist to the subject, wherein the administration of the binding agent is prior to, simultaneously with, or after administration of the PD-1 axis binding antagonist.

100. The binding agent and / or PD-1 axis binding antagonist for use of any one of claims 97 to 99, wherein the method for treating or preventing a disease or disorder in a subject is a method for reducing or preventing progression of a tumor or treating cancer in a subject.

101. Use of a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen- binding region binding to CD137 for the manufacture of a medicament for treating or preventing a disease or disorder in a subject in combination with a PD-1 axis binding antagonist.

102. Use of a PD-1 axis binding antagonist for the manufacture of a medicament for treating or preventing a disease or disorder in a subject, in combination with a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen-binding region binding to CD137.

103. Use of (i) a binding agent comprising a first antigen-binding region binding to EpCAM and a second antigen- binding region binding to CD137 and (ii) a PD-1 axis binding antagonist for the manufacture of a medicament for treating or preventing a disease or disorder in a subject.

104. The use of any one of claims 101 to 103, wherein the disease or disorder is a tumor, solid tumor or cancer.

105. A medical preparation comprising the binding agent and the PD-1 axis binding antagonist defined in any one of claims 1 to 104.

106. The medical preparation of claim 105 for use in the method of any one of claims 1 to 85.

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