Animal respiratory dust exposure test apparatus and test method

Abstract

An animal respiratory dust exposure test apparatus, comprising a constant temperature and humidity test box (1) internally provided with a test chamber. The constant temperature and humidity test box (1) is provided with a dust inlet and a box door (13); a dust conveying device is connected to the dust inlet; a manure leaking separation net (11) is provided in the test chamber; the manure leaking separation net (11) divides the test chamber into an activity area and a cleaning area which are distributed in the vertical direction; an air blowing device and a tray (12) are provided in the cleaning area; and the air blowing device ensures that dust remains suspended all the time, so as to improve the effect of contact between test animals and dust, and improve the test result authenticity. Also disclosed is a test method, comprising the following steps: S1, putting the test animals and the tray (12) into the activity area and the cleaning area; S2, injecting dust, and starting the air blowing device; S3, adjusting the temperature and the humidity; S4, taking out the test animals every day for weighing, observing the feeding and activity condition, and cleaning the tray (12) and the dust; and S5, repeating the steps S1-S4, drawing blood after several days for biochemical marker assay to complete one test period.

Description

Animal breathing dust test device and test method Technical Field

[0001] The invention belongs to the technical field of pneumoconiosis animal simulation test, and particularly relates to an animal breathing dust contamination test device and a test method thereof. Background Art

[0002] Coal mining generates large amounts of dust, polluting the tunnel environment. Long-term inhalation of dust by workers can lead to pneumoconiosis, a serious threat to their occupational health and safety, and hindering the development of underground coal mining. Due to experimental limitations, conducting dust inhalation experiments on coal miners is difficult, hindering subsequent pathological research. To this end, researchers have begun using pneumoconiosis rats to simulate the effects of dust inhalation on human body functions. For example, patent number 201920282288.4, entitled "Animal Husbandry Experimental Device Under Dust Exposure," discloses a method for placing rats in a sealed chamber and introducing dust into the chamber to simulate the mine environment. The chamber is equipped with a manure-leaking screen and a particle concentration sensor. The screen divides the chamber into two upper and lower areas. The upper area is used for rat movement and feeding, while the lower area is equipped with a manure conveyor to transport feces. A dust conveyor is connected to the chamber to provide a preset concentration of dust to simulate the mine environment. However, the above patent has many problems: First, as the test time increases, the floating dust will gradually fall and settle on the bottom plate of the cabin, which will make the contact effect between rats and dust worse, and cannot well simulate the process of human body contact with dust, and thus cannot effectively carry out subsequent research work; Second, there is a lack of simulation of harmful gases and other components in coal mines, and it is impossible to explore the harm caused by harmful gases to the human body. Moreover, pneumoconiosis is often caused by multiple factors such as dust and harmful gases. Therefore, it is impossible to truly evaluate the harm caused by the environment in coal mines to rats.

[0003] Summary of the Invention

[0004] The purpose of the present invention is to provide an animal respiratory dust exposure test device and a test method thereof. After dust is provided in the test chamber, the blowing equipment can ensure that the dust is always diffused in the test chamber, thereby improving the contact effect between the test animals and the dust, better simulating the process of human body contact with dust, and making the test results more realistic.

[0005] The technical solution adopted by the present invention is an animal respiratory dust pollution test device, comprising a constant temperature and humidity test box with a test chamber therein, the constant temperature and humidity test box being provided with a dust inlet connected to the test chamber, the dust inlet being connected to a dust conveying device, a manure-leaking isolation net being provided in the test chamber, the manure-leaking isolation net dividing the test chamber into a cleaning area and an activity area for animal activities, the activity area and the cleaning area being distributed up and down, the cleaning area being provided with a blowing device for blowing air and dust and a tray for receiving feces and urine, the constant temperature and humidity test box being provided with a box door connected to both the activity area and the cleaning area.

[0006] The present invention is also characterized in that:

[0007] Preferably, the constant temperature and humidity test box is provided with an exhaust gas inlet communicated with the test chamber, and the exhaust gas inlet is connected to an exhaust gas supply device.

[0008] Preferably, the exhaust gas supply equipment includes an exhaust gas storage tank containing mixed exhaust gas, and a flow meter and a flow regulating valve are sequentially connected between the exhaust gas storage tank and the exhaust gas inlet.

[0009] Preferably, the test chamber is provided with a dust concentration monitor, an exhaust gas concentration monitor, a thermometer and a hygrometer.

[0010] Preferably, the dust conveying equipment includes a powder feeder and a pulverizer, the pulverizer is located above the powder feeder, and the powder outlet of the pulverizer is connected to the powder storage barrel of the powder feeder.

[0011] Preferably, a dryer is connected between the powder feeder and the dust inlet.

[0012] Preferably, the constant temperature and humidity test box is provided with an exhaust port communicating with the test chamber, and the exhaust port is connected to a dust removal device.

[0013] Preferably, a transparent window is provided on the box door for allowing light to penetrate into the activity area and the cleaning area, and a curtain is provided on the transparent window.

[0014] Also disclosed is a method for an animal respiratory dust contamination test, which uses the above-mentioned animal respiratory dust contamination test device and includes the following steps:

[0015] S1. Open the chamber door, place the test animal and the tray into the activity area and the cleaning area of ​​the test chamber respectively, and close the chamber door;

[0016] S2, turning on the dust conveying device to inject a preset concentration of dust into the test chamber, and after the injection is completed, turning off the dust conveying device and turning on the blowing device to raise the dust;

[0017] S3, adjusting the temperature and humidity in the test chamber to a preset temperature and humidity;

[0018] S4. After the preset test time is reached each day, the test animal is taken out of the chamber door, and the dust in the tray and the test chamber is cleaned. The removed test animal is weighed and its eating and activity status are observed;

[0019] S5. Repeat steps S1 to S4. After a few days, take blood from the test animals to perform biochemical tests, completing one test cycle.

[0020] Preferably, in step S2, while turning on the dust conveying equipment, the exhaust gas supply equipment is turned on to inject mixed exhaust gas of a preset concentration into the test chamber. After the dust conveying equipment and the exhaust gas supply equipment have completed the injection, the blowing equipment is turned on to raise dust.

[0021] Beneficial effects of the present invention:

[0022] 1. In the present invention, after providing simulated dust to the test chamber in the constant temperature and humidity test box, turning on the blowing device can lift the dust in the test chamber, so that the dust is always diffused in the chamber, avoiding dust deposition, which makes the contact effect between the experimental animals and the dust worse and cannot well simulate the process of human body contact with dust.

[0023] 2. In the present invention, in addition to providing simulated dust for the test chamber in the constant temperature and humidity test box, simulated exhaust gas is also provided for the test chamber, so that the simulated environment in which the test animals are located is closer to the real environment, making the simulation test more accurate and improving the authenticity of the simulation.

[0024] 3. In the present invention, a dryer is provided between the powder feeder and the dust inlet, which can dry the dust in advance to reduce the impact of humidity on the test environment.

[0025] 4. In the present invention, transparent windows and curtains are set up on the door. Opening the curtains can simulate the light environment, such as the harm caused to the human body in a flour mill, and closing the curtains can simulate the dark environment, such as the harm caused to the human body in a coal mine. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a schematic diagram of the structure of the animal breathing dust test device.

[0027] In the figure: 1. Constant temperature and humidity test chamber; 2. Powder feeder; 3. Dryer; 4. Crusher; 5. Dust removal equipment; 6. Induced draft fan; 7. Exhaust gas storage tank; 8. Exhaust gas inlet pipe; 9. Dust inlet pipe; 10. Exhaust pipe; 11. Leakage manure isolation net; 12. Tray; 13. Chamber door; 14. Transparent window; 15. Curtain; 16. Flow meter; 17. Flow regulating valve; 18. Dust concentration monitor; 19. Exhaust gas concentration monitor; 20. Thermometer; 21. Hygrometer. DETAILED DESCRIPTION

[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0029] Example 1

[0030] The present embodiment provides an animal respiratory dust test device, as shown in Figure 1, comprising a constant temperature and humidity test chamber 1, an exhaust gas supply device, and a dust conveying device. A test chamber is provided in the constant temperature and humidity test chamber 1, and a dust inlet is provided outside the constant temperature and humidity test chamber 1, and the dust inlet is connected to the test chamber. The dust inlet is connected to the dust conveying device through a dust inlet pipe 9, and the dust conveying device provides dust for the test chamber. The dust components, proportions, and concentrations need to be proportioned according to the simulated environment. A horizontally arranged manure isolation net 11 is provided in the test chamber, and the manure isolation net 11 divides the test chamber into an activity area and a cleaning area distributed up and down, that is, the area above the manure isolation net 11 is the activity area, which is used to place test animals, such as mice. Below the leaky manure isolation net 11 is a cleaning area, in which a blowing device (not shown) and a tray 12 are provided. The blowing device can be started after the dust supply stops to lift the dust in the test chamber, avoid dust deposition, and reduce the contact effect between the test animals and the dust. There is no requirement for the specific location and number of the blowing device, as long as the dust in the test chamber can be blown up. Therefore, even if the test chamber is filled with blowing devices, the wind force of the blowing device does not need to be very large, as long as the dust can be diffused in the air. The tray 12 is used to receive the feces and urine leaked from the leaky manure isolation net 11, making it convenient for later cleaning. The constant temperature and humidity test chamber 1 is provided with a door 13, which is connected to the active area and cleaning area of ​​the test chamber. The door 13 is provided on the one hand to take and place the test animals and the tray 12, and on the other hand to be able to replace fresh air for the test chamber and clean the dust inside so that the next test can be carried out. The blowing equipment can be a fan, of course, other blowers can also be used. When using a fan, it is installed at the bottom of the cleaning area. The number of fans is set according to actual needs. In order to ensure that the wind can fully cover the test chamber, the fan direction can be set at multiple angles, such as upward, downward and towards other wind directions. The fan wind force does not need to be too large, it only needs to ensure that the dust can be effectively lifted and diffused in the air.

[0031] Working principle:

[0032] Open the door 13, place the tray 12 and the test animals (such as white mice, gray mice, etc.) in the cleaning area and the activity area respectively, then close the door 13, turn on the dust conveying device, inject a preset concentration of dust into the test air, and stop injecting after reaching the corresponding concentration. Start the blowing device and the constant temperature and humidity test box 1. The blowing device blows the dust in the test chamber so that it is diffused in the air. Through the control panel of the constant temperature and humidity test box 1, adjust the humidity and temperature in the test chamber to reach the preset simulated temperature and humidity. Take the test animals out of the test chamber every day, measure their weight, and observe their eating and activity. The test animals can stay in the test chamber for a few hours every day, without having to stay in the test chamber all day, to avoid hypoxia and severe lack of food affecting the test data. Then, after repeating the above steps for several days, take the test animals out for blood drawing to perform biochemical tests. The dust preset concentration can be preset according to the actual environment you want to simulate, or different concentrations can be set for comparison.

[0033] This animal respiration dust test device can be used to simulate the working environment of underground coal mines and evaluate the survival index of coal mines in harsh environments. For example, if the dust conveying equipment is conveying a mixed powder such as coal powder and quartz sand powder, it can maximize the simulation of the underground coal mine environment. Of course, it can also be used to simulate other dusty environments, such as flour mills.

[0034] In order to simulate the air environment in the environment and explore the harm that harmful gases and dust bring to the human body, in this embodiment, as shown in Figure 1, an exhaust gas inlet is also provided outside the constant temperature and humidity test box 1, and the exhaust gas inlet is connected to the test chamber. The exhaust gas inlet is connected to the exhaust gas supply equipment through the exhaust gas inlet pipe 8 to supply mixed exhaust gas into the test chamber. The components and proportions of the mixed exhaust gas need to be proportioned according to the simulated environment. The blowing equipment is restarted after the exhaust gas and dust are stopped. When the animal respiratory dust test device is used to simulate the working environment underground in a coal mine, the exhaust gas supply equipment provides CO, CO2, nitrogen oxides NO X Such gases are commonly found in coal mines.

[0035] Furthermore, in this embodiment, as shown in FIG1 , the tail gas supply equipment includes a tail gas storage tank 7 , which contains a mixed tail gas. When used for coal mine underground environment simulation, the mixed tail gas is CO, CO2, nitrogen oxides NO Xand other gases commonly found in coal mines. A flow meter 16 and a flow regulating valve 17 are sequentially connected between the tail gas storage tank 7 and the tail gas inlet, that is, a flow meter 16 and a flow regulating valve 17 are sequentially provided on the tail gas inlet pipe 8. The flow meter 16 is used to monitor the amount of tail gas supplied, and the flow regulating valve 17 is used to adjust the flow rate to adjust the amount of tail gas supplied. Preferably, the tail gas storage tank 7 is made of stainless steel and pre-loaded with mixed simulated tail gas. The tail gas storage tank 7 has a volume of 4L and a working pressure of 6MPa. An electromagnetic stop valve is provided on the discharge port of the tail gas storage tank 7, and the electromagnetic stop valve is connected to the tail gas inlet pipe 8. The electromagnetic stop valve is a DJ2B type electromagnetic stop valve, which is suitable for various gas systems to control the opening and closing of gases, including the opening and closing control of general corrosive gases, and has good corrosion resistance and good sealing.

[0036] In this embodiment, as shown in FIG1 , a dust concentration monitor 18, an exhaust gas concentration monitor 19, a thermometer 20, and a hygrometer 21 are provided in the test chamber to monitor the dust concentration, exhaust gas concentration, humidity, and temperature in the test chamber. Preferably, a PLC programmable logic controller can be pre-installed on the constant temperature and humidity test chamber 1, and the data of each dust concentration monitor 18, exhaust gas concentration monitor 19, thermometer 20, hygrometer 21, and flow meter 16 can be collected through the PLC editing program, and the powder feeder 2, dryer 3, crusher 4, and flow control valve 17 can be controlled accordingly. After the various data are preset on the control panel, the dust concentration, exhaust gas concentration, temperature, humidity, etc. can be automatically adjusted. Preferably, the dust concentration monitor 18 can use the SDS036 industrial dust sensor, which is made based on the principle of laser scattering. It is used for monitoring heavy pollution and high concentrations, with a range of 0 to 1000 mg / m 3 The exhaust gas concentration monitor 19 can measure the concentration range: 10-2000ppm, with a resolution of 1ppm, a response time of 15s, and a circuit voltage of ≤15VDC. The programmable logic controller PLC can be a Mitsubishi FX3U-16MT PLC.

[0037] In this embodiment, as shown in Figure 1, the dust conveying equipment includes a powder feeder 2 and a crusher 4. The output end of the powder feeder 2 is connected to the dust inlet pipe 9. The crusher 4 is located above the powder feeder 2, and the powder outlet of the crusher 4 is connected to the powder storage barrel of the powder feeder 2. The powder feeder 2 is a single-cylinder air-borne powder feeder that uses carrier gas to convey powder, and is used to convey powdered materials. Common powder feeders can convey powder particles with a diameter of about 20-250μm, with a powder feeding amount error of less than 2%, and a repeated powder feeding amount error of less than 1%. Only inert gases such as argon, nitrogen, and helium can be used as powder-carrying gases, and oxygen, hydrogen, and other combustible gases cannot be used. The basic structural functions of the powder feeder include: a powder storage barrel, a transmission device, a control device, and an air circuit system. For example, the Aigang IGS series powder feeder, manufactured by Henan Coal Research Institute Wear-Resistant Technology Co., Ltd., has a powder storage barrel made of plexiglass placed at the top of the device. A servo-controlled gate valve with PID control is used to control the dust flow rate. The air system uses a compressor to compress air, which enters through one end of the gate valve, driving the dust into the model. A servo-controlled PID control valve is used to adjust the opening between powder feeder 2 and the dust-generating point to control the dust generation rate. When used for underground coal mine environment simulation, the coal and quartz sand must first be crushed by crusher 4. The crushed powder is then discharged through the powder outlet into the powder storage barrel of powder feeder 2. The degree of crushing must be between 30 and 300 mesh.

[0038] In this embodiment, as shown in Figure 1, a dryer 3 is connected between the powder feeder 2 and the dust inlet. That is, the dust inlet pipe 9 is also provided with a dryer 3 to dry the dust in advance.

[0039] To facilitate dust removal within the test chamber, in this embodiment, as shown in FIG1 , a constant temperature and humidity test chamber 1 is provided with an exhaust port, which communicates with the test chamber and is connected to a dust removal device 5 via an exhaust pipe 10. The dust removal device 5 is capable of extracting exhaust gas from the test chamber and performing dust removal. When setting up the dust removal device 5, a mechanical dust collector, a wet dust collector, an electrostatic precipitator, or a bag filter can be selected based on the actual dust type and budget. The outlet of the dust removal device 5 is connected to an induced draft fan 6 to extract air from the test chamber and then remove the dust removal device 5. When the dust removal device 5 and induced draft fan 6 are activated, the chamber door 13 must be opened to allow air to flow in through the chamber door 13 and out through the exhaust pipe 10, forming an air flow that removes dust from the test chamber. This also prevents the chamber door 13 from being closed, which could result in excessive negative pressure within the test chamber and cause deformation of the constant temperature and humidity test chamber 1.

[0040] In this embodiment, as shown in FIG1 , a transparent window 14 is provided on the door 13 to allow light to penetrate into the activity area and the cleaning area. A curtain 15 is provided on the transparent window 14. Closing the curtain 15 can simulate a dark environment, such as an underground coal mine, while opening the curtain 15 can simulate a bright environment, such as a flour mill.

[0041] Furthermore, in this embodiment, as shown in Figure 1, a constant temperature and humidity test chamber 1 utilizes an existing constant temperature and humidity chamber. The specific test chamber dimensions of the constant temperature and humidity test chamber 1 are 380mm (length x width x height) x 400mm (height) x 520mm, with a capacity of 80L. The temperature control range is 0-60°C, with a temperature fluctuation of ±1°C, and a humidity range of 45-90% RH. Because the constant temperature and humidity test chamber 1 utilizes existing technology, it will not be elaborated upon in detail. A brief description will be provided: the chamber's inner liner is constructed of stainless steel, while the outer shell is plastic-sprayed carbon steel, creating an aesthetically pleasing appearance. The temperature and humidity are adjustable and controllable, and the control panel features an LCD digital display. A spray inlet is located on the side of the chamber to control humidity, and the chamber is equipped with an insulation layer and a heating device, such as a heating rod.

[0042] Example 2

[0043] This embodiment provides an animal respiratory dust contamination test method, which uses the animal respiratory dust contamination test device in Example 1, as shown in Figure 1, and includes the following steps:

[0044] S1. Open the door 13, place the test animal and the tray 12 into the activity area and the cleaning area of ​​the test chamber respectively, and close the door 13.

[0045] S2. Turn on the dust conveying device to inject a preset concentration of dust into the test chamber. After the injection is completed, turn off the dust conveying device and turn on the blowing device to raise the dust;

[0046] S3. Adjust the temperature and humidity in the test chamber to the preset temperature and humidity;

[0047] S4. After the preset test time is reached each day, the chamber door 13 is opened to remove the test animal, and the dust in the tray 12 and the test chamber is cleaned. The removed test animal is weighed and its eating and activity status are observed;

[0048] S5. Return the test animal and the tray 12 to the activity area and the cleaning area, and repeat steps S1 to S4. After a few days, take blood from the test animal for biochemical index testing, completing one test cycle.

[0049] Furthermore, in this embodiment, as shown in FIG1 , in step S2 , while the dust conveying equipment is turned on, the exhaust gas supply equipment is turned on to inject a mixed exhaust gas of a preset concentration into the test chamber. After the dust conveying equipment and the exhaust gas supply equipment are both injected, the blowing equipment is turned on to raise the dust.

[0050] Specifically, in this embodiment, as shown in FIG1 , taking mice as an example, simulating an underground coal mine environment includes the following steps:

[0051] First, the box door 13 is opened, and the mouse and the tray 12 are placed in the activity area and the cleaning area of ​​the test chamber respectively, and the box door 13 is closed.

[0052] Then, add coal blocks and quartz sand into the crusher 4 and crush them to about 200 meshes. The ratio of coal blocks and quartz sand is determined according to the actual site. Then, the mixed dust is passed into the powder storage cylinder of the powder feeder 2. Then, the powder is blown into the dust inlet pipe 9 under the power of the compressed air source of the air compressor. Then, it is blown into the test chamber after being dried by the dryer 3. The stop valve of the tail gas storage tank 7 is opened, and the flow regulating valve 17 is adjusted according to the monitoring of the flow meter 16 to inject the mixed tail gas into the test chamber. When the dust concentration monitor 18 and the tail gas concentration monitor 19 monitor that the dust concentration and tail gas concentration in the test chamber reach the preset value, the powder feeder 2 and the tail gas storage tank 7 are stopped, and the fan is turned on to raise the dust.

[0053] Then, the constant temperature and humidity test chamber 1 is started to adjust the temperature and humidity in the test chamber. When the data monitored by the thermometer 20 and the hygrometer 21 reach the preset values, the adjustment of the constant temperature and humidity test chamber 1 is stopped.

[0054] Then, draw the curtain 15 to simulate the dark environment of the mine. After the mice have been in the test chamber for 8 hours every day, open the door 13 to take the mice out, weigh them, and observe their eating and activity. Then, take out the cleaning tray 12 for cleaning, turn on the induced draft fan 6 and dust removal equipment 5, and extract the air in the test chamber to remove dust. If the suction is not complete, the test chamber can also be cleaned manually.

[0055] Finally, the mice and the tray 12 are returned to the activity area and the cleaning area, and steps S1 to S4 are repeated. After 5 to 7 days, blood is collected from the mice, and then biochemical indicators are tested to complete one test cycle.

[0056] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. An animal breathing dust test device, characterized in that: The invention comprises a constant temperature and humidity test box with a test chamber therein, wherein the constant temperature and humidity test box is provided with a dust inlet connected to the test chamber, the dust inlet is connected to a dust conveying device, a manure-leaking isolation net is provided in the test chamber, the manure-leaking isolation net divides the test chamber into a cleaning area and an activity area for animals, the activity area and the cleaning area are distributed up and down, a blowing device for blowing dust and a tray for receiving feces and urine are provided in the cleaning area, and the constant temperature and humidity test box is provided with a box door connected to both the activity area and the cleaning area.

2. The animal breathing dust test device according to claim 1, characterized in that: The constant temperature and humidity test box is provided with an exhaust gas inlet communicated with the test chamber, and the exhaust gas inlet is connected to an exhaust gas supply device.

3. The animal breathing dust test device according to claim 2, characterized in that: The tail gas supply equipment comprises a tail gas storage tank filled with mixed tail gas, and a flow meter and a flow regulating valve are connected in sequence between the tail gas storage tank and the tail gas inlet.

4. The animal breathing dust test device according to claim 3, characterized in that: The test chamber is provided with a dust concentration monitor, an exhaust gas concentration monitor, a thermometer and a hygrometer.

5. The animal breathing dust test device according to claim 4, characterized in that: The dust conveying equipment comprises a powder feeder and a pulverizer, wherein the pulverizer is located above the powder feeder, and a powder outlet of the pulverizer is communicated with a powder storage cylinder of the powder feeder.

6. The animal breathing dust test device according to claim 5, characterized in that: A dryer is connected between the powder feeder and the dust inlet.

7. The animal breathing dust test device according to claim 1, characterized in that: The constant temperature and humidity test box is provided with an exhaust port communicated with the test chamber, and the exhaust port is connected to a dust removal device.

8. The animal breathing dust test device according to claim 1, characterized in that: The box door is provided with a transparent window for light to penetrate into the activity area and the cleaning area, and the transparent window is provided with a curtain.

9. An animal breathing dust test method, using an animal breathing dust test device as claimed in claims 1 to 8, characterized in that: The following steps are involved: S1. Open the door, place the test animal and the tray into the activity area and the cleaning area of ​​the test chamber respectively, and close the door; S2, turning on the dust conveying device to inject a preset concentration of dust into the test chamber, and after the injection is completed, turning off the dust conveying device and turning on the blowing device to raise dust; S3, adjusting the temperature and humidity in the test chamber to a preset temperature and humidity; S4. After the preset test time is reached every day, the box door is opened to take out the test animals, and the dust in the tray and the test chamber is cleaned. The taken-out test animals are weighed, and their eating and activity conditions are observed; S5. Repeat steps S1 to S4. After a few days, take blood from the test animals to test biochemical indicators, completing one test cycle.

10. The method for animal breathing dust test according to claim 9, characterized in that: In step S2, while turning on the dust conveying device, the exhaust gas supply device is turned on to inject mixed exhaust gas of a preset concentration into the test chamber. After the dust conveying device and the exhaust gas supply device have completed the injection, the blowing device is turned on to raise dust.

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