Genetic sources for increasing soybean resistance to soybean cyst nematode hg type 1.2.5.7

Abstract

The invention relates to genes which may be utilized to induce resistance to soybean cyst nematode (SCN). More specifically the present disclosure provides genes that, when inactivated, modified, or overexpressed in a plant, particularly, a soybean plant, can confer upon the plant resistance to SCN. Methods of using these genes to obtain plants, particularly, soybean plants, that are resistant to SCN are also provided.

Description

[0001] GENETIC SOURCES FOR INCREASING SOYBEAN RESISTANCE TO SOYBEAN

[0002] CYST NEMATODE HG TYPE 1.2.5.7

[0003] CROSS-REFERENCE TO RELATED APPLICATION

[0004] This application claims the benefit of U.S. Provisional Application Serial No. 63 / 601,885, filed November 22, 2023, the disclosure of which is hereby incorporated by reference in its entirety, including all figures, tables and amino acid or nucleic acid sequences.

[0005] STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH

[0006] This invention was made with government support under Award # 2023-67013-39162 awarded by the United States Department of Agriculture. The government has certain rights in the invention.

[0007] STATEMENT REGARDING SEQUENCE LISTING

[0008] The Sequence Listing for this application is labeled “Seq-List.xml” which was created on November 14, 2024 and is 61,390 bytes. The entire content of the sequence listing is incorporated herein by reference in its entirety.

[0009] BACKGROUND OF THE INVENTION

[0010] Soybean is a major agricultural crop in the United States, with 33.3 million hectares harvested in 2020 (USDA NASS, 2021). Despite numerous efforts that have been made to optimize and improve soybean yield, significant yield losses are often observed in nearly all soybean growing areas as a result of infection by soybean cyst nematode (SCN, Heterodera glycines). SCN is the most yield-limiting and economically important pathogen of soybeans in the United States, with an average annual yield losses estimated at $1.5 billion (Koenning and Wrather, 2010; Tylka, 2021; Tylka and Marett, 2021). Since the first report in the US in 1995 (Winstead et al., 1955), SCN has spread to almost every soybean producing county (Tylka and Marett, 2021).

[0011] SCN is a sedentary parasite of soybean roots and is considered the most serious pathogen problem in soybean production. In order to maintain a sedentary lifestyle, the parasitic SCN juveniles induce the formation of a multinucleated feeding site: the syncytium. Under optimal conditions, the SCN life cycle can be completed in about 4 weeks, and several generations can be produced during a single growing season. An SCN infection interrupts root vascular tissue function, resulting in stunted growth and severe yield losses. The field management of an SCN infection is complicated by the fact that the infection does not always induce distinct above-ground symptoms, even when causing significant yield loss.

[0012] The most efficient strategy to control SCN is the use of resistant soybean cultivars. However, given the narrow genetic base of SCN resistance in commercial soybean cultivars grown in the United States together with the high genetic diversity of field populations of SCN, SCN populations have evolved to overcome resistance in these cultivars. Some SCN populations are able to reproduce and cause yield losses in SCN-resistant cultivars to a similar extent to SCN-susceptible cultivars (Niblack et al., 2008; McCarville et al., 2017; Tylka et al., 2021). The continued use of soybean cultivars with PI 88788 and Peking-derived resistance will not only be ineffective but will lead to increases in SCN virulence and population densities. Numerous surveys assessed the virulence of SCN populations relative to the sources of resistance used in a large number of commercially available soybean cultivars; there was increased virulence coming from the PI 88788 source of resistance and measurable seed yield losses on cultivars that were previously resistant to SCN (McCarville etal., 2017). In addition, Heterodera glycines (HG) type testing revealed that virulence phenotypes of SCN have shifted, and Hg Type 1.2.5.7 (race 2) became as widely distributed as Hg Type 0 (race 3) (Mitchum et al., 2007). This is not unexpected, since PI 88788 and Peking, the main sources of SCN resistance in commercially cultivated cultivars, are highly susceptible to several HG types (Niblack et al., 2002). Despite the emergence of SCN Hg Type 1.2.5.7 as one of the most common Hg Types in several Eastern and Southern States (Young, 1990; Koenning and Barker, 1998; Mitchum etal., 2007) and limited resistance for this Hg Type in commercially-available soybean cultivars, soybean genes conferring resistance to this Hg Type remain unidentified.

[0013] Genetic mapping of SCN resistance genes or trait loci during the last three decades have resulted in the identification of several genomic regions controlling SCN resistance dispersed throughout almost all of the 20 soybean chromosomes. This highlights the complexity of the inheritance of SCN resistance trait and the difficulty of identifying the underlying genes. Concibido etal., (2004), for instance, identified 31 quantitative trait loci (QTL) associated with SCN resistance on 18 chromosomes. Similarly, numerous QTL linked to SCN resistance in cultivated soybean and wild-type accessions have been reported (Glover et al., 2004; Kazi et al., 2010; Vuong et al., 2010; Kim et al., 2011; Kim and Diers, 2013; Bao et al., 2014; Han et al., 2015; Vuong et al., 2015; Kadam et al., 2016; Zhang etal., 2016; Zhang et al., 2017; Tran et al., 2019). Despite the progress in identifying QTL linked to SCN resistance, cloning the underlying resistance genes and elucidating the molecular mechanisms controlling soybean resistance to various Hg Types remains challenging. Thus far, only two major loci conferring resistance to SCN have been identified on chromosomes 18 (Rhgl) and 8 (Rhg4) (Cook et al., 2012; Liu et al., 2012). The Rhg4 locus contains one gene encoding serine hydroxymethyltransferase (GmSHMT08) (Liu et al., 2012). In contrast, the Rhgl locus contains three genes within a 31 kb genomic region that contribute to SCN resistance in an additive manner. These genes encode for an a- SNAP protein (Gm SNAP 18), a woundinducible domain protein (WI12), and an amino acid transporter (Cook et al., 2012).

[0014] Resistance to SCN in commercial soybean cultivars grown in the United States is obtained from two main sources: Plant Introduction (PI) 88788 and PI 548402. PI 88788, containing the rhgl-b resistance allele, is the main source of SCN resistance in more than 95% of SCN-resistant commercial cultivars growing in the United States. PI 548402 (Peking), containing the rhgl-a and Rhg4 resistance alleles, is the second most common source of resistance deployed in about 5% of SCN-resistant commercial cultivars. Peking-type SCN- resistant is bigenic requiring both rhgl-a (GmSNAP18) and Rhg4 (GmSHMT08) (Liu et al., 2012; Kandoth et al., 2017; Patil et al., 2019), while PI88788-type resistance requires at least 7 copies of the 31-kb rhgl-b repeat region (Cook et al., 2014). Notably, continuous use of soybean cultivars with the same source of resistance during the last 30 years has led to SCN populations evolving and overcoming PI 88788- and Peking-derived resistance (McCarville et al. 2017; Tylka et al., 2021). In addition, PI 88788- and Peking-derived resistance is effective only against Hg Type 0, adding an additional limitation to using these accessions as the sole source of SCN resistance in commercial soybean cultivars.

[0015] Soybean resistance to various HG types are mediated by different genes. Therefore, developing soybean cultivars with broad-spectrum resistance to SCN requires identification of major SCN resistance genes against various HG Types of SCN.

[0016] BRIEF SUMMARY OF THE INVENTION

[0017] The instant invention pertains to a gene modification of Glyma.l4G050600 Glyma.l6G137000, Glyma.l4G050700, Glyma.l4G056000.2, Glyma.03G054100,

[0018] Glyma.l0G285300, Glyma.04G225500, Glyma.l2G216900, Glyma.U032805,

[0019] Glyma.03G253600, Glyma.04G 185400, Glyma.l8g244600, or any combination thereof or an expression modification to Glyma.l4G050600 Glyma.l6G 137000, Glyma.l4G050700, Glyma.l4G056000.2, Glyma.03G054100, Glyma.l0G285300, Glyma.04G225500, Glyma.12G216900, Glyma. U032805, Glyma.03G253600, Glyma.04G185400,

[0020] Glyma.18g244600, or any combination thereof that increases soybean resistance to SCN. Thus, the subject invention enables generating improved soybean cultivars with broad-spectrum and durable resistance against multiple SCN Hg-types via traditional breeding, genome editing, or transgenic approaches.

[0021] BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication, with color drawing(s), will be provided by the Office upon request and payment of the necessary fee.

[0023] FIGs. 1A-1C. Resistance to SCN Hg Type 1.2.5.7 is not controlled by Rhgl or Rhg4 resistance genes. FIG. 1A: Distribution of female index (FI) for SCN Hg Type 1.2.5.7 in the recombinant inbred line (RIL) population. FIGs. IB and 1C: Copy number variation of Rhgl and Rhg4 genomic intervals based on CGH data. Each spot indicates the log2 ratios of each genotype compared to Williams 82 for a specific CGH probe. A value of zero indicates that there is no difference between the sample and Williams 82 (which has one copy for each locus). The different genotypes are represented by spot color (Red: TN09-029 (Pl); Blue: NC05-1168 (P2); Green: RIL- 109 (R); Black: RIL-099 (MR); Yellow: RIL-066 (S). B: All five genotypes show approximately three copies of the Rhgl interval. FIG. 1C: All five genotypes show one copy (matching Williams 82) of the Rhg4 interval.

[0024] FIG. 2. Gene expression levels of three candidate resistance genes in response to infection by SCN Hg Type 1.2.5.7. RT-qPCR quantification of the three candidate resistance genes (Glyma.14G050600, Glyma.14G050700, and Glyma.14G056000.2) in susceptible (RIL- 072, represented by the left bar graph in each double bar graph) and resistant (RIL-137, represented by the right bar graph in each double bar graph) RILs at 5-day post infection with SCN Hg Type 1.2.5.7. Relative fold change values represent changes in infected roots compared to the corresponding non-infected roots.

[0025] FIG. 3. Glyma.14G050600 significantly increased soybean resistance to SCN Hg Type 1.2.5.7. The resistant and susceptible variants of Glyma.14G050600 (PDIL) was overexpressed in the susceptible line RIL-72 using transgenic hairy root system. Transgenic hairy root expressing the empty vector were used as control. Composite hairy root plants overexpressing the resistant variant of Glyma.14G050700 was also used as an additional control for comparison. The cyst index was calculated by dividing the average number of cysts determined on the transgenic hairy root plants by the average number of cysts on the control plants expressing the empty vector, which was set to 100%. Data represent mean ± SE of at least 20 plants. Asterisks indicate statistically significant differences from control plants expressing the empty vector at p < 0.05 as determined by ANOVA.

[0026] FIG. 4. Transcriptome changes induced by SCN Hg Type 1.2.5.7 in resistant and susceptible RILs. Venn diagram showing overlaps between upregulated and downregulated genes identified in resistant (RIL-137) and susceptible (RIL-72) lines using RNA-seq data.

[0027] FIG. 5. Silencing the expression of Glyma.03G054100 (SEQ ID NO: 17), Glyma.04G225500 (SEQ ID NO: 21), and Glyma.l0G285300 (SEQ ID NO: 19) significantly increased soybean resistance to SCN Hg Type 1.2.5.7. About 300 bp DNA fragments specific to Glyma.03 G054100 (SEQ ID NO: 17), Glyma.04G225500 (SEQ ID NO: 21), Glyma.10G285300 (SEQ ID NO: 19), and Glyma.l2G216900 (SEQ ID NO: 29) were synthesized and cloned in the gene silencing vector pTRV2. The 4 pTRV2 vectors in addition to empty vector (control) were introduced Agrobacterium tumefaciens strain GV2260 and used to infiltrate five-day-old soybean seedlings of the susceptible soybean line RIL-72. Twelve days after agrobacterium inoculation, agrobacterium inoculation, each soybean seedling was inoculated with about 2000 eggs of SCN Hg Type 1.2.5.7. Five weeks after inoculation, SCN cysts were extracted from each plant separately and used to calculate cyst index, and subsequently plant resistance. Data represent mean ± SE of at least 20 plants. Asterisks indicate statistically significant differences from control plants expressing the empty TRV2 vector determined by ANOVA with p < 0.05.

[0028] BRIEF DESCRIPTION OF THE SEQUENCES

[0029] SEQ ID NO: 1: Nucleotide sequence encoding Glyma.14G050600 Susceptible RIL. SEQ ID NO: 2: Nucleotide sequence encoding Glyma.14G050700 Susceptible RIL. SEQ ID NO: 3: Nucleotide sequence encoding Glyma.l4G056000.2 Susceptible RIL. SEQ ID NO: 4: Nucleotide sequence encoding Glyma.16G137000 Susceptible RIL. SEQ ID NO: 5: Nucleotide sequence encoding Glyma.14G050600 Resistant RIL. SEQ ID NO: 6: Nucleotide sequence encoding Glyma.14G050700 Resistant RIL. SEQ ID NO: 7: Nucleotide sequence encoding Glyma.l4G056000.2 Resistant RIL. SEQ ID NO: 8: Nucleotide sequence encoding Glyma.16G137000 Resistant RIL. SEQ ID NO: 9: Amino acid sequence encoding Glyma.14G050600 Susceptible RIL. SEQ ID NO: 10: Amino acid sequence encoding Glyma.14G050700 Susceptible RIL. SEQ ID NO: 11: Amino acid sequence encoding Glyma.l4G056000.2 Susceptible

[0030] RIL.

[0031] SEQ ID NO: 12: Amino acid sequence encoding Glyma.l6G137000 Susceptible RIL.

[0032] SEQ ID NO: 13: Amino acid sequence encoding Glyma.l4G050600 Resistant RIL.

[0033] SEQ ID NO: 14: Amino acid sequence encoding Glyma.l4G050700 Resistant RIL.

[0034] SEQ ID NO: 15: Amino acid sequence encoding Glyma.l4G056000.2 Resistant RIL.

[0035] SEQ ID NO: 16: Amino acid sequence encoding Glyma.l6G137000 Resistant RIL.

[0036] SEQ ID NO: 17: Nucleotide sequence encoding Glyma.03G054100.

[0037] SEQ ID NO: 18: Amino acid sequence encoding Glyma.03G054100.

[0038] SEQ ID NO: 19: Nucleotide sequence encoding Glyma. l0G285300.

[0039] SEQ ID NO: 20: Amino acid sequence encoding Glyma.l0G285300.

[0040] SEQ ID NO: 21: Nucleotide sequence encoding Glyma.04G225500.

[0041] SEQ ID NO: 22: Amino acid sequence encoding Glyma.04G225500.

[0042] SEQ ID NO: 23: Nucleotide sequence encoding Glyma.03G253600.

[0043] SEQ ID NO: 24: Amino acid sequence encoding Glyma.03G253600.

[0044] SEQ ID NO: 25: Nucleotide sequence encoding Glyma.04Gl 85400.

[0045] SEQ ID NO: 26: Amino acid sequence encoding Glyma.04Gl 85400.

[0046] SEQ ID NO: 27: Nucleotide sequence encoding Glyma. l8G244600.

[0047] SEQ ID NO: 28: Amino acid sequence encoding Glyma.l8G244600.

[0048] SEQ ID NO: 29: Nucleotide sequence encoding Glyma. l2G216900.

[0049] SEQ ID NO: 30: Amino acid sequence encoding Glyma.l2G216900.

[0050] SEQ ID NO: 31: Nucleotide sequence encoding Glyma.U032805.

[0051] SEQ ID NO: 32: Amino acid sequence encoding Glyma.U032805.

[0052] DETAILED DISCLOSURE OF THE INVENTION

[0053] The present invention relates to a novel and useful methods for introducing, in a reliable and predictable manner, soybean cyst nematode (SCN) resistance into non-resistant soybean germplasm. The method involves the genetic-mapping of genes associated with SCN resistance based on the analysis of lines that are resistant or susceptible to SCN. The genetic loci identified to be associated with the SCN resistance can be modified, inactivated, or have increased or decreased expression in a plant to render the plant resistant to SCN.

[0054] In this disclosure the term “isolated nucleic acid” molecule means a nucleic acid molecule that is separated from other nucleic acid molecules that are usually associated with the isolated nucleic acid molecule. Thus, an “isolated nucleic acid molecule” includes, without limitation, a nucleic acid molecule that is free of nucleotide sequences that naturally flank one or both ends of the nucleic acid in the genome of the organism from which the isolated nucleic acid is derived (e.g., a cDNA or genomic DNA fragment produced by PCR or restriction endonuclease digestion). Such an isolated nucleic acid molecule is generally introduced into a vector (e.g., a cloning vector or an expression vector) for convenience of manipulation or to generate a fusion nucleic acid molecule. In addition, an isolated nucleic acid molecule can include an engineered nucleic acid molecule such as a recombinant or a synthetic nucleic acid molecule. A nucleic acid molecule existing among hundreds to millions of other nucleic acid molecules within, for example, a nucleic acid library (e.g., a cDNA or genomic library) or a gel (e.g., agarose, or polyacrylamine) containing restriction-digested genomic DNA, is not an “isolated nucleic acid”.

[0055] The term “nucleic acid” or “polynucleotide” refers to deoxyribonucleic acids (DNA) or ribonucleic acids (RNA) and polymers thereof in either single- or double-stranded form. Unless specifically limited, the term encompasses nucleic acids containing known analogs of natural nucleotides that have similar binding properties as the reference nucleic acid and are metabolized in a manner similar to naturally occurring nucleotides. Unless otherwise indicated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g., degenerate codon substitutions), alleles, orthologs, single nucleotide polymorphisms (SNPs), and complementary sequences as well as the sequence explicitly indicated. Specifically, degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and / or deoxyinosine residues (Batzer et al., Nucleic Acid Res. 19:5081 (1991); Ohtsuka et al., J. Biol. Chem. 260:2605-2608 (1985); and Rossolini et al., Mol. Cell. Probes 8:91-98 (1994)). The term nucleic acid is used interchangeably with gene, cDNA, and mRNA encoded by a gene.

[0056] The term “gene” means the segment of DNA involved in producing a polypeptide chain, messenger RNA (mRNA), or a microRNA (miRNA); it includes regions preceding and following the coding region (leader and trailer) involved in the transcription / translation of the gene product and the regulation of the transcription / translation, as well as intervening sequences (introns) between individual coding segments (exons).

[0057] In this application, the terms “polypeptide”, “peptide”, and “protein” are used interchangeably herein to refer to a polymer of amino acids. The terms apply to amino acid polymers in which one or more amino acid residues are artificial chemical mimetic of a corresponding naturally occurring amino acids, as well as to naturally occurring amino acid polymers and non-naturally occurring amino acid polymers. As used herein, the terms encompass amino acid chains of any length, including full-length proteins, wherein the amino acid residues are linked by covalent peptide bonds.

[0058] As used in herein, the terms “identical” or percent “identity”, in the context of describing two or more polynucleotide or amino acid sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same (for example, a variant protein used in the method of this invention has at least 80% sequence identity, preferably 85%, 90%, 91%, 92%, 93, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity, to a reference sequence), when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection. Such sequences are then said to be “substantially identical”. With regard to polynucleotide sequences, this definition also refers to the complement of a test sequence.

[0059] For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters. For sequence comparison of nucleic acids and proteins, the BLAST and BLAST 2.0 algorithms and the default parameters discussed below are used.

[0060] As used herein, the singular forms “a”, “an” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise. Further, to the extent that the terms “including”, “includes”, “having”, “has”, “with”, or variants thereof are used in either the detailed description and / or the claims, such terms are intended to be inclusive in a manner similar to the term “comprising”. The transitional terms / phrases (and any grammatical variations thereof) “comprising”, “comprises”, “comprise”, include the phrases “consisting essentially of’, “consists essentially of’, “consisting”, and “consists”. The phrases “consisting essentially of’ or “consists essentially of’ indicate that the claim encompasses embodiments containing the specified materials or steps and those that do not materially affect the basic and novel characteristic(s) of the claim.

[0061] The term “about” means within an acceptable error range for the particular value as determined by one of ordinary skill in the art, which will depend in part on how the value is measured or determined, z.e., the limitations of the measurement system. Where particular values are described in the application and claims, unless otherwise stated the term “about” meaning within an acceptable error range for the particular value should be assumed.

[0062] In the present disclosure, ranges are stated in shorthand, to avoid having to set out at length and describe each and every value within the range. Any appropriate value within the range can be selected, where appropriate, as the upper value, lower value, or the terminus of the range. For example, a range of 1-10 represents the terminal values of 1 and 10, as well as the intermediate values of 2, 3, 4, 5, 6, 7, 8, 9, and all intermediate ranges encompassed within 1-10, such as 2-5, 2-8, and 7-10. Also, when ranges are used herein, combinations and subcombinations of ranges (e.g., subranges within the disclosed range) and specific embodiments therein are intended to be explicitly included.

[0063] An endogenous nucleic acid is a nucleic acid that is naturally present in a cell. For example, a nucleic acid present in the genomic DNA of a cell is an endogenous nucleic acid.

[0064] An exogenous nucleic acid is any nucleic acid that is not naturally present in a cell. For example, a nucleic acid vector introduced into a cell constitutes an exogenous nucleic acid.

[0065] An endogenous promoter is a promoter that is naturally associated with a specific gene within a cell. In contrast, an exogenous promoter is any promoter that is not naturally associated with a specific gene within a cell. For example, the soybean ubiquitin promoter is considered an exogenous promoter relative to the genes or nucleic acid sequences encoding any one of SEQ ID NOs: 1-8, 17, 19, 21, 23, 25, 27, 29, or 31.

[0066] The subject invention provides for the use of “homologous nucleic acid sequences” or “homologs of nucleic acid sequences”. Homologs of nucleic acid sequences will be understood to mean any nucleotide sequence obtained by mutagenesis according to techniques well known to persons skilled in the art, and exhibiting modifications in relation to the parent sequences. For example, mutations in the regulatory and / or promoter sequences for the expression of a polypeptide that result in a modification of the level of expression of a polypeptide according to the invention provide for a “homolog of a nucleotide sequence”. Likewise, substitutions, deletions, or additions of nucleic acid to the polynucleotides of the invention provide for “homologs” of nucleotide sequences. In various embodiments, “homologs” of nucleic acid sequences have substantially the same biological activity as the corresponding reference gene, i.e., a gene homologous to a native gene would encode for a protein having the same biological activity as the corresponding protein encoded by the naturally occurring gene. Typically, a homolog of a gene shares a sequence identity with the gene of at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%. These percentages are purely statistical and differences between two nucleic acid sequences can be distributed randomly and over the entire sequence length.

[0067] Likewise, the subject invention also provides for the use of “homologs of proteins”. Homologs of proteins will be understood to mean any proteins obtained by mutagenesis according to techniques well known to persons skilled in the art, and exhibiting modifications in relation to the parent protein. Such modifications in a protein sequence include substitutions, deletions, or additions of amino acids to produce homologs of proteins. In various embodiments, a homolog of a protein has substantially the same biological activity as the protein, i.e., a protein homolog of a native protein would have the same biological activity as the native protein. Typically, a homolog of a reference protein shares a sequence identity with the reference protein of at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%. These percentages are purely statistical and differences between two nucleic acid sequences can be distributed randomly and over the entire sequence length.

[0068] The instant invention pertains to modifying sequences genes and / or the expression of genes in soybean plants to promote resistance to SCN. In certain embodiments, the sequences of Glyma.14G050600, Glyma.16G 137000, Glyma.14G050700, Glyma.l4G056000.2, Glyma.03G253600, Glyma.04G185400, Gly ma.18g244600, or any combination thereof can be modified to promote soybean resistance to SCN, including, for example SCN Hg Type 1.2.5.7. In certain embodiments, the modification to Glyma.l4G050600, Glyma. 16G 137000. Glyma. 14G050700. Glyma.l4G056000.2, or any combination thereof can further comprise a modification of the expression of Glyma.14G050600, Glyma.16G 137000. Glyma. 14G050700. Glyma.14G056000.2, Glyma.03G253600, Glyma.04G185400, Glyma.18g244600, or any combination thereof. In certain embodiments, the modifications to the nucleotide sequence and, optionally, expression of a gene enables generating improved soybean cultivars with broad- spectrum and durable resistance against multiple SCN Hg-types via traditional breeding, genome editing, or transgenic approaches. In certain embodiments, the instant invention pertains to novel recombinant inbred lines (RILs) segregating for resistance to SCN Hg Type 1.2.5.7 and quantitative trait loci (QTL) associated with resistance. Whole-genome sequencing of RILs and fine-mapping resulted in the identification of a genes mediating soybean response to SCN.

[0069] In this context, PI 437654 represents an excellent source for identifying new SCN resistance genes against various Hg Types including HG type 1.2.5.7 (race 2), the emerging Hg Type in Eastern and Southern States (Mitchum etal., 2007). PI 437654 is resistant to almost all SCN Hg Types (Anand 1988; Diers et al., 1997). Despite the fact that several QTL associated with SCN resistance have been identified in various cultivated and wild soybean accessions (Yue et al., 2001; Guo et al., 2005; Winter et al., 2007; Vuong et al., 2010), these QTL have not been functionally validated and the genes underpinning resistance have not been identified. Consistent with the broad resistance of PI 437654 to several Hg Types, several QTL for SCN resistance have been mapped from PI 437654 as compared with those mapped from PI 88788 (Webb et al., 1995; Concibido et al., 2004; Wu et al., 2009; Kim et al., 2011). For example, Wu et al. (2009) detected several major and minor QTL associated with SCN resistance to Hg Types 2.5.7, 1.2.5.7, 0, and 2.7 (races 1, 2, 3, and 5, respectively) from PI 437654. These findings point to the presence of multiple SCN resistance alleles in PI 437654 that may function in a specific and / or additive fashion against various Hg Types.

[0070] The disclosure provides that the genes listed in Table 1 (SEQ ID NOs: 1-8) and SEQ ID NOs: 17, 19, 21, 23, 25, 27, 29, and / or 31 provide resistance or susceptibility to SCN in a plant cell or a plant, particularly, a soybean plant cell or a soybean plant. For example, genetically modifying in a plant cell or a plant, particularly, a soybean plant cell or a soybean plant, to incorporate one or more genes comprising a protein coding sequence selected from SEQ ID NOs: 5-8, SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27 renders the plant cell or the plant, particularly, the soybean plant cell or the soybean plant, resistant to SCN. Table 1: SEQ ID NOs: 1 to 8 providing candidate genes that confer SCN resistance when overexpressed or inactivated.

[0071] SEQ ID NO: 1 : Nucleotide sequence encoding Glyma.14G050600 conferring susceptible RIL

[0072] ATGCCGAAGTCTCAATTTCGAACCCCATTTCTGGTTTCTCTTCCCCTTTTGTTATTC ATCTTCAACCTCACCCCTTCGCACGCACTCTATGGAGCATCCTCACCCGTGCTTC

[0073] AACTCACTCCCTCTAACTTCAAGTCCAAGGTTCTGAATTCAAATGGAGTTGTTCTT

[0074] GTTGAATTCTTTGCTCCATGGTGTGGACACTGTCAGGCTCTGACTCCTATATGGG

[0075] AGAAGGCAGCTACTGTGTTGAAGGGTGTTGTTACTGTGGCAGCAATTGATGCTGA

[0076] TGCTCACCCGTCTTTGGCTCAGGAATATGGAATTAGAGGATTTCCAACTATAAAA GTGTTTGC ACCTGGAAAGCC ACCTGTTGATTACC AAGGAGC AAGAGATGTC AAA

[0077] CCAATTGCTGAATTTGCACTTCAACAGGTAAAGGCTCTTTTGAAGGATCGGTTAA

[0078] GTGGAAAAGCAACAGGAGGATCTAGTGACAAGACAGAAACCAGTTCTTCAGTAG

[0079] AATTGAACTCTGGCAACTTTGATGAATTGGTGATCAAAAGCAAAGAACTCTGGAT TGTGGAATTTTTCGCACCTTGGTGTGGACATTGTAAGAAATTAGCTCCTGAGTGG AAGAAAGCATCCAATAGTTTGAAAGGGAAGGTTAAACTGGGCCATGTTGACTGT GATGCTGAAAAGTCTCTAATGAGCAGGTTCAAAGTTCAAGGATTCCCAACTATCT TGGTGTTTGGTGCTGATAAAGATAGTCCTATTCCTTATGAAGGCGCAAGAACTGC CTTGGCTATTGAATCATTTGCATTAGAGCAGCTGGAAACAAACGTTGCTCCTCCA GAAGTGACAGAGCTACACAGTCCAGATGTTTTGGAAGAGAAATGTGGTTCTGCC GCAATCTGTTTTGTTGCCTTCCTTCCTGACATTTTAGATTCCAAGGCTGAGGGGAG AAACATATATCTTCAGCAGTTACTATCTGTTGCAGAGAAGTTTAAAAGGAGTCCA TACAGCTACGTCTGGGTAGCTGCAGGGAATCAGCCAGATCTTGAGAAGAATGTG GGTGTTGGAGGGTACGGTTATCCAGCTTTAGTGGCCCTTAACCTTAAGAAAGCTG TTTATGCTCCTCTCAAGAGTGCTTTTGAACTTGACCAGATTATAGAATTTGTGAAA GAAGCTGGACGTGGAGGCAAAGGGAATTTGCCCCTGCAAGGCACTCCAACCATT GTAAAGACAGAACCATGGGATGGAAAAGATGGAGAAATAATTGAGGAGGATGA ATTTTCTCTTGAAGAACTAATGGGGGAAGATGCTTCAAGCAAGGATGAGCTATG A

[0080] SEQ ID NO: 9 : Amino acid sequence encoding Glyma.14G050600 conferring susceptible RIL MPKSQFRTPFLVSLPLLLFIFNLTPSHALYGASSPVLQLTPSNFKSKVLNSNGVVLVEF FAPWCGHCQALTPIWEKAATVLKGVVTVAAIDADAHPSLAQEYGIRGFPTIKVFAPG KPPVDYQGARDVKPIAEFALQQVKALLKDRLSGKATGGSSDKTETSSSVELNSGNFD ELVIKSKELWIVEFFAPWCGHCKKLAPEWKKASNSLKGKVKLGHVDCDAEKSLMSR FKVQGFPTILVFGADKDSPIPYEGARTALAIESFALEQLETNVAPPEVTELHSPDVLEE KCGSAAICFVAFLPDILDSKAEGRNIYLQQLLSVAEKFKRSPYSYVWVAAGNQPDLE KNVGVGGYGYPALVALNLKKAVYAPLKSAFELDQIIEFVKEAGRGGKGNLPLQGTP TIVKTEPWDGKDGEIIEEDEF SLEELMGED AS SKDEL

[0081] SEQ ID NO: 2 : Nucleotide sequence encoding Glyma.14G050700 conferring susceptible RIL ATGGAGTCTCTCTTCGACTCGATCAACGTCCGCGACCTTCTTTCGGCGCAGGATC TCTCGGACCCTAACTCCCCTCTCTCGGCGCCGGATCTCCGTCTCCTGATCCAGCGC CTGGAGTCGCACTCCCTCCAAATCCGCTCTCAGGTGCAGTCCTACCTCGTCTCGC ACCGCGAGGATTTCGCGCGCCTCTTTTCCCTCTGCAACGACGCTGTTTCGCAGAC GCGCGAGGTCTCCGACGACGTCACCGCCATCCTCCGACTCCTCTCCGACCGCCCG ATTGACGCCGAGGTGCGCGACATCGTGTCGGAGATGAAGGCAAAGAAGGAGGA ACTGAAAGTGAAGAAGGAACTGCTGGGATTGGTTGGAACCGTTGTGGCGCTGAA

[0082] CCAGAGATTGGAGAGTGTGAGGGAAGCGTTGAAGAGTGGGAGGTTCGAATTCGC

[0083] GGCGCAAGGGTTGAAGGAGTTGAAGGTGGCGTTGAGGATTGGTGAGGAGAATGA

[0084] TAGGGAGCCTTTGGTGTATGGCTTGTTGAGGAAGGAGTGGTCTCAGTGCTTTGAA

[0085] GAGATTCAAGAGGTCCTCATGAAGTTTATGGAAAAGGCAGTACGATTTGATGGG

[0086] GATTTGAATCAAGTTGAAGTCAAGTATCATTTAGAAGTTGAGAATGTGAATGGG

[0087] ATTCAATTACACACAGTTGTGGAGGCAATGGATGTAGTTGGTATACTAGAGTATG

[0088] GGCTTGCTAAAGTTGCTGATTTGATGATCAAGTATGTCATTACTCCTTTTGTAAAT

[0089] CATGGACAACCTCTTTCATTTCTTGAGGAATTGCATCAAGAATCGGCTCTACTGA

[0090] AGATAGTTCCATCACTAGATAGTAAGTTTGAATATTTGGATGGGGAGTTTCTCTA

[0091] TTCAAGGATTCTGCTGTTTATCAAATTTATTTACAGAAGTATTTGCTTCCAGAAAA

[0092] GTTCTTGGATGCAGTGTTTTGGACGGTTGACATGGCCAAGGATATCAGAGCTAAT

[0093] AATATCTACCTTTCTTTCAAAGGTTGTCCCAACAGATGCATCAAAACTTCCTGACT

[0094] TTCAGAAGATCATTGTATGTTCATCTGAATTTGAGACAGCTTTAAAAGAGCTTAT

[0095] GTACATTTCAGCATCAGATGACAAAGATAACAGGCTGAGCAATTTTGCTGAAAA

[0096] TGTTGAGGTTCACTTTGCATTCAAGAAAAAGACAGAGATCTTGGCTAAAGCTAGA

[0097] AACCTACTTCTAGAATGTGACTTTTCAATTCCTCAAGAGTATACAAGGGATGGTT

[0098] CTGTTTGGAAGAGTGATGAAACTTCTGTCCAGTCATCCAGCCATGTGGTAGATTT

[0099] GCTTTTCTTATCAGAGAGGTGTCTAGTATCCAAAGCAGCCAAACAATTGATGGAG

[0100] CTAGTTCATCAGACGCTGCAGGATGTTTGCCTGTCATCTACAAGAGTTGCTTTGG

[0101] AATTTTATCACACAGCTAGAGATGCTATACTACTTTATGAAGTAGTTGTCCCTGTC

[0102] AAGCTAGAGAGGCAGCTCAATGGCATTAATCATGTAGCCGTTCTGTTGCATAATG

[0103] ACTGTCTTTATCTCTCCCAGGAGATATTTGGGTTTGCATTTGAGTATCGAACAGAC

[0104] TTTCCAAGTTCCATGAAGGAACATGCTGTGTTTGTTGATTTGGCTCCAAGGTTTCA

[0105] GCTGTTGGCAGAAGAAATATTGCAGAGACAAGTTCACCTTGTTATTTATAATTTG

[0106] AAGGAGGCTATAGATGGTGCTGATGGATTTCAGAATACTCATCAGATGAAACAA

[0107] TTTGAGTCAGCTAAATTTAGCATAGACCAGGTTGTTTTCATTCTGGAAAAAGTAC

[0108] ATATCATATGGGAGCCGCTTTTGCTGCCTTCAACTTACAGGAGAAGCATGTGTAC

[0109] AGTCTTAGAGTCAGTTTTCTCAAGAATTGCCAGAGATATACTTCTGTTAGATGAC

[0110] ATAGCTGCAGAGGAGACTTTACAGCTACAAAGACTTATTTATTTAATGCTGGAAA

[0111] ACCTGTTGTCATTATTTGAGTCTTTGGCCCCTGGAGAACAGAATTTGCATGAGTTC

[0112] TCTGCAGAGTCTCTTGAAGATTTTATCCCGTCCTTACGTAAAATCCGTAAACTATC

[0113] AGAATTATTAGATATGCCTTTAAAATCCATTACAGCATCTTGGGAGAATAAAGAA TTGCTCTCTTGTGGCTTTACAATAACTGAGGTGGAAGATTTCATAAAAGCTATAT

[0114] TTACAGACTCACCTTTAAGAAAAGATTGTTTATGGAGGATACAAAATCCGAGCTT

[0115] TTAG

[0116] SEQ ID NO: 10: Amino acid sequence encoding Glyma.l4G050700 conferring susceptible RIL

[0117] MESLFDSINVRDLLSAQDLSDPNSPLSAPDLRLLIQRLESHSLQIRSQVQSYLVSHRED FARLFSLCNDAVSQTREVSDDVTAILRLLSDRPIDAEVRDIVSEMKAKKEELKVKKEL LGLVGTVVALNQRLESVREALKSGRFEFAAQGLKELKVALRIGEENDREPLVYGLLR KEWSQCFEEIQEVLMKFMEKAVRFDGDLNQVEVKYHLEVENVNGIQLHTVVEAMD VVGILEYGLAKVADLMIKYVITPFVNHGQPLSFLEELHQESALLKIVPSLDSKFEYLD GEFLYSRILLFIKFIYRSICFQKSSWMQCFGRLTWPRISELIISTFLSKVVPTDASKLPDF QKIIVCSSEFETALKELMYISASDDKDNRLSNFAENVEVHFAFKKKTEILAKARNLLL ECDF SIPQEYTRDGS VWKSDETS VQS S SHVVDLLFLSERCL VSKAAKQLMELVHQTL QDVCLSSTRVALEFYHTARDAILLYEVVVPVKLERQLNGINHVAVLLHNDCLYLSQE IFGFAFEYRTDFPSSMKEHAVFVDLAPRFQLLAEEILQRQVHLVIYNLKEAIDGADGF

[0118] QNTHQMKQFESAKFSIDQVVFILEKVHIIWEPLLLPSTYRRSMCTVLESVFSRIARDIL LLDDIAAEETLQLQRLIYLMLENLLSLFESLAPGEQNLHEFSAESLEDFIPSLRKIRKLS ELLDMPLKSITASWENKELLSCGFTITEVEDFIKAIFTDSPLRKDCLWRIQNPSF

[0119] SEQ ID NO: 3 : Nucleotide sequence encoding Glyma.l4G056000.2 conferring susceptible RIL

[0120] ATGATGAATCACGATTCCAACAACGAACAACATCAGATTCTCCATGTCTCGCAGG

[0121] ACGACGAGATAATGGAATCGCTAATCCTCGATGACGACAGCTCTTCCGTTCAGCA GCACCACCGTCCTACGTCGCCGCAGAGCCCTAATTCCCCTTTCAACTCGTTCCTC GACCCTCCCTCCTACGCCGACGCGATCTTCACCTCCTTCGATTCCAACGGTCACG ACCAAGCCATCGAATCCCCCGCCGCCCGATCCGGCTCCGAAGACTACCTCCACAT CTCCGTCACCGATCCTCAGAAGGAGCAGGACATCGCCACCTCCCTCGTCCCCGGC GCCGCCGCCTTCTACACCTACCTCATCACCACGCGCACTAACCTCCCCGAATATG GCGGCATCGGTGCTGAGTTCGCCGTCCGGCGGCGGTTCCGCGACGTGGTGACACT CTCCGACCGGCTCTCGGAGGCATACCGCGGGTTCTTCATCCCGGTGCGGCCGGAC AAGAGCACGGTGGAGAGCCAGGTGATGCAGAAGCAGGAGTTTGTGGAGCAGCG GCGCGTGGCGTTGGAGAAGTACCTCAGGAAGCTGGCGGCGCACCCGGTGATCGG GCGGAGCGAGGAGCTCAGGTTGTTTCTCGAGGCCAAGGGGCGGCTGCCGCTGGC CAAGACCTTCGATGTCGCGTCGCGAATGCTCGATGGCGCCGTGAGGTTGCCAAG GCAGCTGTTCGGGGGAGAGGCGGAGCTGGGAGAGGTGGCTCAGCCGGCCAAGG GCGGGAGGGACTTGCTCAGGATTTTCAAGGAGTTGAAGCAGTCTGTTGCCAATG ACTGGGTTGGGAGCAAGCCACTTGTTGTGGAGGAGGATAAGGAGTTCATGGAGA GGAAGGATAAGTTGGTGGATTTTGAGCACCATCTTAGCAATGTTTCTCAGCAGGC TGAATCCCTTGTCAAGTTTCAGCAAGACATGGGTGAAACGGTTGGTGAATTAGGT

[0122] CTGGCTTTTGTAAAGCTTACCAAATTTGAGACGGAAGAAGCCATATTTGAGTCTC AGAGAGTTCGAGCTGCTGACATGAGAAATGTGGCAACTGCAGCTGTTAAAGCAA GCAGGTTATATAGAGAGCTGAATACACAGACAATCAAACATTTGGATAAACTAC ATGAATACCTTGGGACAATGCTAGCTGTTAACAATGCATTTTCTGACAGATCGAG TGCATTGTTGACTGTTCAAACACTCTCATCAGAACTAGCTTCTTTACATTCACGGG TGGAAAAACTTGAAGTTGCTTCATCCAAAATATTTGGTGGAGACAAGTCTAGGAT GCGGAAAATTGAAGAGTTAAAAGAAGCCATTAGAGTTACTGAGAATGCTAAAAT

[0123] TTGTGCAGATAGAGAGTATGAACGAATCAAGGAAAATAATAGGAGTGAACTTGA AAGAATTGACCAAGAGAGGAATAGCGACTTCCTAAGCATGCTGCGAGGGTTTGT

[0124] TGTCAATCAAGTAAAGCTCATTTATCTTCTATGTTTTGTAAGCTGTTACGTTTAA

[0125] SEQ ID NO: 11: Amino acid sequence encoding Glyma.l4G056000.2 conferring susceptible RIL

[0126] MMNHDSNNEQHQILHVSQDDEIMESLILDDDSSSVQQHHRPTSPQSPNSPFNSFLDPP SYADAIFTSFDSNGHDQAIESPAARSGSEDYLHISVTDPQKEQDIATSLVPGAAAFYT

[0127] YLITTRTNLPEYGGIGAEFAVRRRFRDVVTLSDRLSEAYRGFFIPVRPDKSTVESQVM QKQEFVEQRRVALEKYLRKLAAHPVIGRSEELRLFLEAKGRLPLAKTFDVASRMLDG AVRLPRQLFGGEAELGEVAQPAKGGRDLLRIFKELKQSVANDWVGSKPLVVEEDKE FMERKDKLVDFEHHLSNVSQQAESLVKFQQDMGETVGELGLAFVKLTKFETEEAIFE SQRVRAADMRNVATAAVI<ASRLYRELNTQTII<HLDI<LHEYLGTMLAVNNAFSDRS SALLTVQTLSSELASLHSRVEKLEVASSKIFGGDKSRMRKIEELKEAIRVTENAKICAD REYERIKENNRSELERIDQERNSDFLSMLRGFVVNQVKLIYLLCFVSCYV

[0128] SEQ ID NO: 4 : Nucleotide sequence encoding Glyma.16G137000 conferring susceptible RIL

[0129] ATGGGTAAAGAAATTGTCAGAAGAGAATCACCGAAAGAGCCTGGGGAGCGTAG

[0130] CAGGTTATGGTCCCATGAGGATATAAATCAAGTTTTACAAGAAAACAAGGGGAC TAGAAAGATTGAAATCATATGTATGAATTTTTCCTCATCTGGAGAAGAAGTAGAA

[0131] TGGGATGGAGATGCCTTCAAGGAGATGAAAAATCTGAAAACACTTATTATCAAG

[0132] AGTGATTGTTTTTCCAAAGGTCCCAAACATCTTCCAAATACTTTAAGAGTATTGG

[0133] AATGGTGGAGATGTCCTTCACAGGAATGGCCACGTAATTTTAACCCAAAGCAACT

[0134] TGCTATATGCAAGTTACCCGAGAGTAGCTTTACGTCACTCGGGTTGGCCCCATTA

[0135] TTTGAAAAGAGGCTCGTGAATTTGACAAGGTTAACTTTGGACGAGTGTGATAGTT

[0136] TAACAGAGATACCAGATGTATCTTGTCTCTCAAATTTGGAAAATTTGTCATTTGG

[0137] AGAGTGTCGGAATTTATTTACAATTCACCATTCAGTTGGTTTATTGGAAAAGCTT

[0138] AAAATCTTGGATGCTCAAGATTGCCCAAAGCTTAAGAGTTTTCCACCGTTGAAGT

[0139] TGACCTCTCTTGAAAGGCTTGAACTTTGGTATTGTTGGAGTCTCGAGAGTTTTTCT

[0140] GAAATATTAGGAAAGATGGAAAATATAACAGAACTTTTCTTGACTGATTGTCCC

[0141] ATAACAAAACTCCCACCTTCATTTCGAAATCTTACTCGGCTTCGATCCTTATGTCT

[0142] TGGACCCCATCACCGAACTGAGCAGTTAATTGACTTTGATGCTGCCACCCTCATT

[0143] CCGAACATCTGCATGATGCCAGAACTAAGTCAAATTGAGTTTGGCGGTTTGCAAT

[0144] TGAGGCTATTGCCTGACGATGTTTTGAAATTGACCTCAGTTGTGTGTCCAAGCAT

[0145] TCGATTTGTTTGTTTCTATTACTGCGACCTGTCAGATGAGCTTCTTCGGCTATTTCT

[0146] CTCATGTTTTGTAAATGTGATAAATTTAAAGCTAACAAGTTGTAAATTCACAGTT

[0147] ATTCCCGAATGCATCAAAGAATGCCGCTTTTTAACTTTCCTTACTTTGGATTATTG

[0148] CGATCGTCTACAAGAAATTAGAGGGATTCCTCCAAACTTGATAAGATTCCGTGCA

[0149] AGGACATGCCCAGCCTTGACTTCCTCAAGCATAAGCATGTTGCTGAATCAGGAAC

[0150] TGCTTGAGGCTCGAGACATTCACTTAATAAGTTTGCCAATAGTAAAGATTCCAGA

[0151] GTGGTTTGAGTGCCAGAGTCGGGGACCGTCAATTTTTTTCTGGTTCCCTAACAAA

[0152] TTCCCAGTAATAACTGTTTGCATTGTTACCTCAGGTCCTAAGAAATATTCCAATTA

[0153] TCTGGTTCTCAACGTGATTATTAATAAGAAACATAAACATCGACATCAACGTTTT

[0154] TATAGTAACGGTTCCAACGCAATACCTTCTACAACTGTTTTTCGTCTTCAAATGAA

[0155] AGATAATTTAGATGAAGAACTATCAAAGAGTGAATGGAACCTTGCAGAGATTGT

[0156] ATGCGAAGATTCGTGGGCCGCATACGGAATCCATGTACTGAAAGAGAAAAGTAG

[0157] CATGGAGGATATTCGATTCAGTGATCCTTGCAGAAAAAGAAAAATTTGTAGTTCA

[0158] GAGGTTGGGGTTGGGGAAAAAGCAAAGATTAGTAGGCAGTGA

[0159] SEQ ID NO: 12: Amino acid sequence encoding Glyma.l6G137000 conferring susceptible

[0160] RIL MGKEIVRRESPKEPGERSRLWSHEDINQVLQENKGTRKIEIICMNFSSSGEEVEWDGD AFKEMKNLKTLIIKSDCFSKGPKHLPNTLRVLEWWRCPSQEWPRNFNPKQLAICKLP ESSFTSLGLAPLFEKRLVNLTRLTLDECDSLTEIPDVSCLSNLENLSFGECRNLFTIHHS

[0161] VGLLEKLKILDAQDCPKLKSFPPLKLTSLERLELWYCWSLESFSEILGKMENITELFLT

[0162] DCPITKLPPSFRNLTRLRSLCLGPHHRTEQLIDFDAATLIPNICMMPELSQIEFGGLQLR

[0163] LLPDDVLKLTSVVCPSIRFVCFYYCDLSDELLRLFLSCFVNVINLKLTSCKFTVIPECIK

[0164] ECRFLTFLTLDYCDRLQEIRGIPPNLIRFRARTCPALTSSSISMLLNQELLEARDIHLISL PIVKIPEWFECQSRGPSIFFWFPNKFPVITVCIVTSGPKKYSNYLVLNVIINKKHKHRH QRFYSNGSNAIPSTTVFRLQMKDNLDEELSKSEWNLAEIVCEDSWAAYGIHVLKEKS SMEDIRF SDPCRKRKIC S SE VGVGEK AKISRQ

[0165] SEQ ID NO: 5 : Nucleotide sequence encoding Glyma.l4G050600 conferring resistant SIL

[0166] ATGCCGAAGTCTCAATTTCGAACCCCATTTCTGGTTTCTTTTCCCCTTTTGTTATTC

[0167] ATCTTCAACCTCACCCCTTTGCACGCACTCTATGGAGCATCCTCACCCGTGCTTCA

[0168] ACTCACTCCCTCTAACTTCAAGTCCAAGGTTCTGAATTCAAATGGAGTTGTTCTTG

[0169] TTGAATTCTTTGCTCCATGGTGTGGACACTGTCAGGCTCTGACTCCTATATGGGA

[0170] GAAGGCAGCTACTGTGTTGAAGGGTGTTGTTACTGTGGCAGCAATTGATGCTGAT

[0171] GCTCACCCGTCTTTGGCTCAGGAATATGGAATTAGAGGATTTCCAACTATAAAAG

[0172] TGTTTGCACCTGGAAAGCCACCTGTTGATTACCAAGGAGCAAGAGATGTCAAAC

[0173] CAATTGCTGAATTTGCACTTCAACAGGTAAAGGCTCTTTTGAAGGATCGGTTAAG

[0174] TGGAAAAGCAACAGGAGGATCTAGTGACAAGACAGAAACCAGTTCTTCAGTAGA

[0175] ATTGAACTCTGGCAACTTTGATGAATTGGTGATCAAAAGCAAAGAACTCTGGATT

[0176] GTGGAATTTTTCGCACCTTGGTGTGGACATTGTAAGAAATTAGCTCCTGAGTGGA

[0177] AGAAAGCATCCAATAGTTTGAAAGGGAAGGTTAAACTGGGCCATGTTGACTGTG

[0178] ATGCTGAAAAGTCTCTAATGAGCAGGTTCAAAGTTCAAGGATTCCCAACTATCTT

[0179] GGTGTTTGGTGCTGATAAAGATAGTCCTATTCCTTATGAAGGCGCAAGAACTGCC

[0180] TTGGCTATTGAATCATTTGCATTAGAGCAGCTGGAAACAAACGTTGCTCCTCCAG

[0181] AAGTGACAGAGCTACACAGTCCAGATGTTTTGGAAGAGAAATGTGGTTCTGCCG CAATCTGTTTTGTTGCCTTCCTTCCTGACATTTTAGATTCCAAGGCTGAGGGGAGA

[0182] AACATATATCTTCAGCAGTTACTATCTGTTGCAGAGAAGTTTAAAAGGAGTCCAT

[0183] ACAGCTACGTCTGGGTAGCTGCAGGGAATCAGCCAGATCTTGAGAAGAATGTGG GTGTTGGAGGGTACGGTTATCCAGCTTTAGTGGCCCTTAACCTTAAGAAAGCTGT TTATGCTCCTCTCAAGAGTGCTTTTGAACTTGACCAGATTATAGAATTTGTGAAA GAAGCTGGACGTGGAGGCAAAGGGAATTTGCCCCTGCAAGGCACTCCAACCATT TTAAAGACAGAACCATGGGATGGAAAAGATGGAGAAATAATTGAGGAGGATGA ATTTTCTCTTGAAGAACTAATGGGGGAAGATGCTTCAAGCAAGGATGAGCTATG A

[0184] SEQ ID NO: 13: Amino acid sequence encoding Glyma.l4G050600 conferring resistant SIL MPKSQFRTPFLVSFPLLLFIFNLTPLHALYGASSPVLQLTPSNFKSKVLNSNGVVLVEF FAPWCGHCQALTPIWEKAATVLKGVVTVAAIDADAHPSLAQEYGIRGFPTIKVFAPG KPPVDYQGARDVKPIAEFALQQVKALLKDRLSGKATGGSSDKTETSSSVELNSGNFD ELVIKSKELWIVEFFAPWCGHCKKLAPEWKKASNSLKGKVKLGHVDCDAEKSLMSR FKVQGFPTILVFGADKDSPIPYEGARTALAIESFALEQLETNVAPPEVTELHSPDVLEE KCGSAAICFVAFLPDILDSKAEGRNIYLQQLLSVAEKFKRSPYSYVWVAAGNQPDLE KNVGVGGYGYPALVALNLKKAVYAPLKSAFELDQIIEFVKEAGRGGKGNLPLQGTP TILKTEPWDGKDGEIIEEDEFSLEELMGEDASSKDEL

[0185] SEQ ID NO: 6 : Nucleotide sequence encoding Glyma.l4G050700 conferring resistant SIL ATGGAGTCTCTCTTCGACTCGATCAACGTCCGCGACCTTCTTTCGGCGCAGGATC TCTCGGACCCTAACTCCCCTCTCTCGGCGCCGGATCTCCGTCTCCTGATCCAGCGC CTGGAGTCGCACTCCCTCCAAATCCGCTCTCAGGTGCAGTCCTACCTCGTCTCGC ACCGCGAGGATTTCGCGCGCCTCTTTTCCCTCTGCAACGACGCTGTTTCGCAGAC GCGCGAGGTCTCCGACGACGTCACCGCCATCCTCCGACTCCTCTCCGACCGCCCG ATTGACGCCGAGGTGCGCGACATCGTGTCGGAGATGAAGGCAAAGAAGGAGGA ACTGAAAGTGAAGAAGGAACTACTGGGATTGGTTGGAACCGTTGTGGCGCTGAA CCAGAGATTGGAGAGTGTGAGTGAAGCGTTGAAGAGTGGGAGGTTCGAATTCGC

[0186] GGCGCAAGGGTTGAAGGAGTTGAAGGTGGCGTTGAGGATTGGTGAGGAGAATGA TAGGGAGCCTTTGGTGTATGGCTTATTGAGGAAGGAGTGGTCTCAGTGCTTTGAA GAGATTCAAGAGGTCCTTATGAAGTTTATGGAAAAGGCAGTACGATTTGATGGG GATTTGAATCAAGTTGAAGTCAAGTATCATTTAGAAGTTGAGAATGTGAATGGG ATTCAATTACACACAGTTGTGGAGGCAATGGATGTAGTTGGTATTCTAGAGTATG GGCTTGCTAAAGTTGCTGATTTGATGATCAAGTATGTCATTACTCCTTTTGTAAAT CATGGACAACCTCTTTCATTTCTTGAGGAATTGCATCAAGAATCGGCTCTACTGA AGATAGTTCCATCACTAGATAGTAAGTTTGAATATTTGGATGGAGAGTTTCTCTA TTCAGGGATTCTGCTGTTTATCAAATTTATTTACAGAAGTATTTGCTTCCAGAAAA GTTCTTGGATGCAGTGTTTTGGACGGTTGACATGGCCAAGGATATCAGAGCTAAT AATATCTAGCTTTCTTTCAAAGGTTGTCCCAACAGATGCATCAAAACTTCCTGAC TTTCAGAAGATCATTGTATGTTCATCTGAATTTGAGACAGCTTTAAAAGAGCTTA TGTACATTTCAGCATCAGATGACAAAGATAACAGGCTGAGCAATTTTGCTGAAA ATGTTGAGGTTCACTTTGCATTCAAGAAAAAGACAGAGATCTTGGCTAAAGCTAG AAACCTACTTCTAGAATGTGACTTTTCAATTCCTCAAGAGTATACAAGGGATGGT TCTGTTTGGAAGAGTGATGAAACTTCTGTCCAGTCATCCAGCCATGTGGTAGATT

[0187] TGCTTTTCTTATCAGAGAGGTGTCTAGTATCCAAAGCAGCCAAACAATTGATGGA GCTAGTTCATCAGACGCTGCAGGATGTTTGCCTGTCATCTACAAGAGTTGCTTTG GAATTTTATCACACAGCTAGAGATGCTATACTACTTTATGAAGTAGTTGTCCCTG TCAAGCTAGAGAGGCAGCTCAATGGCATTAATCATGTAGCCGTTCTGTTGCATAA TGACTGTCTTTATCTCTCCCAGGAGATATTTGGGTTTGCATTTGAGTATCGAACAG ACTTTCCAAGTTCCATGAAGGAACATGCTGTGTTTGTTGATTTGGCTCCAAGGTTT CAGCTGTTGGCAGAAGAAATATTGCAGAGACAAGTTCACCTTGTTATTTATAATT

[0188] TGAAGGAGGCTATAGATGGTGCTGATGGATTTCAGAATACTCATCAGATGAAAC AATTTGAGTCAGCTAAATTTAGCATAGACCAGGTTGTTTTCATTCTGGAAAAAGT ACATATCATATGGGAGCCGCTTTTGCTGCCTTCAACTTACAGGAGAAGCATGTGT ACAGTCTTAGAGTCAGTTTTCTCAAGAATTGCCAGAGATATACTTCTGTTAGATG ACATAGCTGCAGAGGAGACTTTACAGCTACAAAGACTTATTTATTTAATGCTGGA AAACCTGTCGTCATTATTTGAGTCTTTGGCCCCTGGAGAACAGAATTTGCATGAG TTCTCTGCAGAGTCTCTTGAAGATTTTATCCCGTCCTTACGTAAAATCCGTAAACT

[0189] ATCAGAATTATTAGATATGCCTTTAAAATCCATTACAGCATCTTGGGAGAATAAA GAATTGCTCTCTTGTGGCTTTACAATAACTGAGGTGGAAGATTTCATAAAAGCTA TATTTACAGACTCACCTTTAAGAAAAGATTGTTTATGGAGGATACAAAATCCGAG CTTTTAG

[0190] SEQ ID NO: 14: Amino acid sequence encoding Glyma.l4G050700 conferring resistant SIL MESLFDSINVRDLLSAQDLSDPNSPLSAPDLRLLIQRLESHSLQIRSQVQSYLVSHRED FARLFSLCNDAVSQTREVSDDVTAILRLLSDRPIDAEVRDIVSEMKAKKEELKVKKEL LGLVGTVVALNQRLESVSEALKSGRFEFAAQGLKELKVALRIGEENDREPLVYGLLR KEWSQCFEEIQEVLMKFMEKAVRFDGDLNQVEVKYHLEVENVNGIQLHTVVEAMD VVGILEYGLAKVADLMIKYVITPFVNHGQPLSFLEELHQESALLKIVPSLDSKFEYLD GEFL YSGILLFIKFIYRSICFQKS SWMQCFGRLTWPRISELIIS SFLSKVVPTD ASKLPDF QKIIVCSSEFETALKELMYISASDDKDNRLSNFAENVEVHFAFKKKTEILAKARNLLL ECDF SIPQEYTRDGS VWKSDETS VQS S SHVVDLLFLSERCL VSKAAKQLMELVHQTL QDVCLSSTRVALEFYHTARDAILLYEVVVPVKLERQLNGINHVAVLLHNDCLYLSQE IFGFAFEYRTDFPSSMKEHAVFVDLAPRFQLLAEEILQRQVHLVIYNLKEAIDGADGF QNTHQMKQFESAKFSIDQVVFILEKVHIIWEPLLLPSTYRRSMCTVLESVFSRIARDIL LLDDIAAEETLQLQRLIYLMLENLSSLFESLAPGEQNLHEFSAESLEDFIPSLRKIRKLS ELLDMPLKSITASWENKELLSCGFTITEVEDFIKAIFTDSPLRKDCLWRIQNPSF

[0191] SEQ ID NO: 7 : Nucleotide sequence encoding Glyma.l4G056000.2 conferring resistant SIL ATGATGAATCACGATTCCAACAACGAACAACATCAGATTCTCCATGTCTCGCAGG ACGACGAGATAATGGAATCGCTAATCCTCGATGACGACAGCTCTTCCGTTCAGCA GCACCACCGTCCTACGTCGCCGCAGAGCCCTAATTCCCCTTTCAACTCGTTCCTC GACCCTCCCTCCTACGCCGACGCGATCTTCACCTCCTTCGATTCCAACGGTCACG ACCAAGCCATCGAATCCCCCGCCGCCCGATCCGGCTCCGAAGACTACCTCCACAT CTCCGTCACCGATCCTCAGAAGGAGCAGGACATCGCCACCTCCCTCGTCCCCGGC

[0192] GCCGCCGCCTTCTACACCTACCTCATCACCACGCGCACTAACCTCCCCGAATATG GCGGCATCGGTGCTGAGTTCGCCGTCCGGCGGCGGTTCCGCGACGTGGTGACACT

[0193] CTCCGACCGGCTCTCGGAGGCATACCGCGGGTTCTTCATCCCGGTGCGGCCGGAC AAGAGCACGGTGGAGAGCCAGGTGATGCAGAAGCAGGAGTTTGTGGAGCAGCG GCGCGTGGCGTTGGAGAAGTACCTCAGGAAGCTGGCGGCGCACCCGGTGATCGG

[0194] GCGGAGCGAGGAGCTCAGGTTGTTTCTCGAGGCCAAGGGGCGGCTGCCGCTGGC CAAGACCTTCGATGTCGCGTCGCGAATGCTCGATGGCGCCGTGAGGTTGCCAAG

[0195] GCAGCTGTTCGGGGGAGAGGCGGAGCTGGGAGAGGTGGCTCAGCCGGCCAAGG GCGGGAGGGACTTGCTCAGGATTTTCAAGGAGTTGAAGCAGTCTGTTGCCAATG ACTGGGTTGGGAGCAAGCCACTTGTTGTGGAGGAGGATAAGGAGTTCATGGAGA

[0196] GGAAGGATAAGTTGGTGGATTTTGAGCACCATCTTAGCAATGTTTCTCAGCAGGC TGAATCCCTTGTCAAGTTTCAGCAAGACATGGGTGAAACGGTTGGTGAATTAGGT CTGGCTTTTGTAAAGCTTACCAAATTTGAGACGGAAGAAGCCATATTTGAGTCTC AGAGAGTTCGAGCTGCTGACATGAGAAATGTGGCAACTGCAGCTGTTAAAGCAA GCAGGTTATATAGAGAGCTGAATACACAGACAATCAAACATTTGGATAAACTAC ATGAATACCTTGGGACAATGCTAGCTGTTAACAATGCATTTTCTGACAGATCGAG

[0197] TGCATTGTTGACTGTTCAAACACTCTCATCAGAACTAGCTTCTTTACATTCACGGG TGGAAAAACTTGAAGTTGCTTCATCCAAAATATTTGGTGGAGACAAGTCTAGGAT GCGGAAAATTGAAGAGTTAAAAGAAGCCATTAGAGTTACTGAGAATGCTAAAAT TTGTGCAGATAGAGAGTATGAACGAATCAAGGAAAATAATAGGAGTGAACTTGA

[0198] AAGAATTGACCAAGAGAGGAATAGCGACTTCCTAAGCATGCTGCGAGGGTTTGT TGTCAATCAAGTAAAGCTCATTTATCTTCTATGTTCTGTATGCTGTTACGTTTAA

[0199] SEQ ID NO: 15: Amino acid sequence encoding Glyma.l4G056000.2 conferring resistant SIL

[0200] MMNHDSNNEQHQILHVSQDDEIMESLILDDDSSSVQQHHRPTSPQSPNSPFNSFLDPP SYADAIFTSFDSNGHDQAIESPAARSGSEDYLHISVTDPQKEQDIATSLVPGAAAFYT YLITTRTNLPEYGGIGAEFAVRRRFRDVVTLSDRLSEAYRGFFIPVRPDKSTVESQVM QKQEFVEQRRVALEKYLRKLAAHPVIGRSEELRLFLEAKGRLPLAKTFDVASRMLDG AVRLPRQLFGGEAELGEVAQPAKGGRDLLRIFKELKQSVANDWVGSKPLVVEEDKE FMERKDKLVDFEHHLSNVSQQAESLVKFQQDMGETVGELGLAFVKLTKFETEEAIFE SQRVRAADMRNVATAAVKASRLYRELNTQTIKHLDKLHEYLGTMLAVNNAFSDRS

[0201] SALLTVQTLSSELASLHSRVEKLEVASSKIFGGDKSRMRKIEELKEAIRVTENAKICAD REYERIKENNRSELERIDQERNSDFLSMLRGFVVNQVKLIYLLCSVCCYV-

[0202] SEQ ID NO: 8 : Nucleotide sequence encoding Glyma.l6G137000 conferring resistant SIL ATGGGTAAAGAAATTGTCAGAAGAGAATCACCGAAAGAGCCTGGGGAGCGTAG

[0203] CAGGTTATGGTCCCATGAGGATATAAATCAAGTTTTACAAGAAAACAAGGGGAC TAGAAAGATTGAAATCATATGTATGAATTTTTCCTCATCTGGAGAAGAAGTAGAA

[0204] TGGGATGGAGATGCCTTCAAGGAGATGAAAAATCTGAAAACACTTATTATCAAG

[0205] AGTGATTGTTTTTCCAAAGGTCCCAAACATCTTCCAAATACTTTAAGAGTATTGG AATGGTGGAGATGTCCTTCACAGGAATGGCCACGTAATTTTAACCCAAAGCAACT

[0206] TGCTATATGCAAGTTACCCAAGAGTAGCTTTACGTCACTCGGGTTGGCCCCATTA TTTGAAAAGAGGCTCGTGAATTTGACAAGGTTAACTTTGGACGAGTGTGATAGTT

[0207] TAACAGAGATACCAGATGTATCTTGTCTCTCAAATTTGGAAAATTTGTCATTTGG AGAGTGTCGGAATTTATTTACAATTCACCATTCAGTTGGTTTATTGGAAAAGCTT

[0208] AAAATCTTGGATGCTCAAGATTGCCCAAAGCTTAAGAGTTTTCCACCGTTGAAGT TGACCTCTCTTGAAAGGCTTGAACTTTGGTATTGTTGGAGTCTCGAGAGTTTTTCT GAAATATTAGGAAAGATGGAAAATATAACACAACTTTTCTGGACTGATTGTCCC ATAACAAAACTCCCACCTTCATTTCGAAATCTTACTCGGCTTCGATCCTTATGTCT TGGACCCCATCACCGAACTGAGCAGTTAATTGACTTTGATGCTGCCACCCTCATT CCGAACATCTGCATGATGCCAGAACTAAGTCAAATTGAGTTTGGCGGTTTGCAAT TGAGGCTATTGCCTGACGATGTTTTGAAATTGACCTCAGTTGTGTGTCCAAGCAT TCGATTTGTTTGTTTCTATTACTGCGACCTGTCAGATGAGCTTCTTCGGCTATTTCT CTCATGTTTTGTAAATGTGATAAATTTAAAGCTAACAAGTTGTAAATTCACAGTT ATTCCCGAATGCATCAAAGAATGCCGCTTTTTAACTTTCCTTACTTTGGATTATTG CGATCGTCTACAAGAAATTAGAGGGATTCCTCCAAACTTGATAAGATTCCGTGCA AGGACATGCCCAGCCTTGACTTCCTCAAGCATAAGCATGTTGCTGAATCAGGAAC TGCTTGAGGCTCGAGACATTCACTTAATAAGTTTGCCAATAGTAAAGATTCCAGA GTGGTTTGAGTGCCAGAGTCGGGGACCGTCAATTTTTTTCTGGTTCCCTAACAAA TTCCCAGTAATAACTGTTTGCATTGTTACCTCAGGTCCTAAGAAATATTCCAATTA TCTGGTTCTCAACGTGATTATTAATAAGAAACATAAACATCGACATCAACGTTTT TATAGTAACGGTTCCAACGCAATACCTTCTACAACTGTTTTTCGTCTTCAAATGAA AGATAATTTAGATGAAGAACTATCAAAGAGTGAATGGAACCTTGCAGAGATTGT ATGCGAAGATTCGTGGGCCGCATACGGAATCCATGTACTGAAAGAGAAAAGTAG CATGGAGGATATTCGATTCAGTGATCCTTGCAGAAAAAGAAAAATTTGTAGTTCA GAGGTTGGGGTTGGGGAAAAAGCAAAGATTAGTAGGCAGTGA

[0209] SEQ ID NO: 16: Amino acid sequence encoding Glyma.l6G137000 conferring resistant SIL MGKEIVRRESPKEPGERSRLWSHEDINQVLQENKGTRKIEIICMNFSSSGEEVEWDGD AFKEMKNLKTLIIKSDCFSKGPKHLPNTLRVLEWWRCPSQEWPRNFNPKQLAICKLP KSSFTSLGLAPLFEKRLVNLTRLTLDECDSLTEIPDVSCLSNLENLSFGECRNLFTIHHS VGLLEKLKILDAQDCPKLKSFPPLKLTSLERLELWYCWSLESFSEILGKMENITQLFW TDCPITKLPPSFRNLTRLRSLCLGPHHRTEQLIDFDAATLIPNICMMPELSQIEFGGLQL RLLPDDVLKLTSVVCPSIRFVCFYYCDLSDELLRLFLSCFVNVINLKLTSCKFTVIPECI KECRFLTFLTLD YCDRLQEIRGIPPNLIRFRARTCPALTS S SISMLLNQELLEARDIHLIS LPIVKIPEWFECQSRGPSIFFWFPNKFPVITVCIVTSGPKKYSNYLVLNVIINKKHKHRH QRFYSNGSNAIPSTTVFRLQMKDNLDEELSKSEWNLAEIVCEDSWAAYGIHVLKEKS SMEDIRF SDPCRKRKIC SSEVGVGEKAKISRQ-

[0210] SEQ ID NO: 17: Nucleotide sequence encoding Glyma.03G054100 conferring susceptible RIL

[0211] ATGTCTTCTTTCCTGTTACGCTTTCCGCCTGAACGGTATCAAGAGGACAACAGAA ACTATGACGTGTTTTTGAGTTTCAGAGGGGACGACACGCGTGCTTCTTTCACTTC ACATCTCTATACCGCTCTTCACAACGCGGGAATCTCTGTTTTCAAGGATGATGAG

[0212] ACACTTCCAAGGGGAAATAAAATTTCAACCTCGCTGGGGTTAGCAATTGAAGAG

[0213] TCTCGACTTTATGTTGTTGTTTTCTCTAAAAACTATGCAGGTTCGCTATGGTGTTT

[0214] GCAAGAGTTGGAGAAAATAATGGAGTGTCACAAAGCAACAGGACAAGTGGTAG

[0215] TGCCAGTGTTCTATGATGTAGATCCCTCTGAAGTACGTCATCAAACAGGCCACTT

[0216] TGGACAAGCATTTCGAAACCTTGAGGCGTATATCAATTTAAAAATGGAAGAGGA

[0217] AATGCAGCCAGGATGGCAAAAGATGGTTCATGAGTGCCCTGGCATCTCAGGGCC

[0218] TTCTGTTTTCCGAGATTGCAATGGTCAAAGTGAGATTTTGGAAAGGATACATCGC

[0219] CTCGTGGAGGATTGGAGGGTGTCACTTCGTAAGATTGTTAGCATCTCCCCCGGTT

[0220] CAGGATTCGGAAAAATGGACGTCGTTGGTAATAAAATAGACAACTTAGTGGAGC

[0221] GTTGGAGGGACGGACTTTGTGTAGCCACTCGCATCCCGTGGAGAGGAATGTTGAT

[0222] TGCTGAACAGTTAATAGATCTCCTTGTGAAGCATTGGAGGACGACACTGAGAAA

[0223] AATAATGGAGATGCCTAGTAATATAGATCTGCTTTTGAAGCATTGGAGTGAGGCA

[0224] CTTTGGAAGGCTGCTGGCATCTCGGGGGGTGCAGACCTAATTTCCAAAAATGAG

[0225] ATGACGGCACCTCTGGACCTTGCGATACACCTCCTCGTGAAGGATTGGAGGAAG

[0226] GCACATCGTGCGGTTCGTGATATCTCAGAGGGTGTAGTCTTAAAATCCATTGTTG

[0227] AAGAGGAGATCAGAAAAGATTTTGAGGTCCTTGTAATTCATTGGAAGGAAGCAC

[0228] TTCATGAGGCTGCTGGCATCTCGAGAATAAGGATTATTGCTGAAGAGTTCGCGAA

[0229] ACATTGGGCAGAGGTACTTCGTGAGGCTGCTAGTATCTCAGGGATTGTAGTCCTA

[0230] AATTCCAGGAATGAAAGTGAGGCTATCAAAACTATTGTTGAAAACGTTAAGCCTT

[0231] TGTTAGACAAGACAGAGTTGTTTGTTGCTGATAATCCAGTCGGCGTAGAACCACG

[0232] AGTCCAGGAAATGATTGAACTATTAGACCAAATACAATCAAACGGTGTTCTACTA

[0233] CTCGGGATGTGGGGGATGGGAGGCATTGGTAAAACAACTATTGCAAAAGCCATT

[0234] TACAACAAGATTGGCCGCAATTTTGAGGTAAAAAGCTTCCTCGCAAGTATTAGGG

[0235] AAGTTTGGGGGCAAGATGCTGGTCAAGTATATCTACAAGAACAACTTATATTTGA

[0236] TATCGGAAAAGAAACAAACACAAAGATACGTAATGTTGACTCAGGAAAAGTTAT

[0237] GTTAAAAGAACGACTTCGCAATAAAAGGGTACTCCTTATACTTGATGATGTAAAT

[0238] AATTTGCATCAATTGAATGTTTTGTGTGGAAGTCGTGAATGGTTTGGTTCAGGGA

[0239] GTAGAATAATAATCACAACTAGAGATATGCATATACTTAGAGGGAGAAGAGTTG

[0240] ACAAAGTGTTCAGAATGAAAGGAATGGATGAAGACGAATCTATTGAGCTTTTTA

[0241] GTTGGCATGCATTTAAGCAAGCAAGTCCAAGAGAAGATTTTATTGAACTTTCTAG

[0242] AAATGTAGTTGCTTATTCTGCGGGATTGCCACTAGCTCTTGAAGTCCTTGGGAAG

[0243] TATTTGTTTGATATGGAGGTAACAGAGTGGAAAAATGTATTGGAGACACTCAAG AAAATTCCTAATGATGAAGTACAAGAGAAATTAAAAATAAGCTATGATGGTTTA ACTGGTGATACAGAGAAAGGAATATTTCTTGATATAGCTTGTTTCTTTACAGGAA

[0244] AGGACCGGAATGATGTTATACATATATTAAATGGTTGTGGGCTTTGTGCAGAAAA

[0245] TGGAATACGTGTCTTGGTAGAAAGAGGCCTTGTAACTGTAGATTATAAGAACAA GCTTGGAATGCATGATTTGCTGCGAGACATGGGAAGAGAAATCATTCGTTCAGA

[0246] AACACCAATGGAGCTTGAGGAGCGTAGTAGGTTATGGTTTCATGAAGATGCGCTT

[0247] GATGTATTATCAAAAGAAACTGGAACAAAAGCTATTGAGGGACTGGCTTTGAAG

[0248] TTACCAAGAACTAATACTAAATGTTTGAGCACTAAAGCTTTTAAGGAGATGAAA AAACTCAGGTTGCTTCAACTTGCTGGTGTACAATTGGTTGGAGATTTCAAGTATC

[0249] TTTCCAAAGATCTTAGATGGCTTTGTTGGCATGGATTTCCTTTAGCATGCATACCA

[0250] ACAAACCTTTATCAAGGAAGTCTAGTTTCCATTGAGCTAGAAAACAGCAATGTTA ATCTTTTGTGGAAAGAAGCCCAGGTAATGGAGAAGCTGAAAATTCTTAATCTCAG TCATTCTCATTATTTAACAGAGACTCCAGACTTTTCAAATTTGCCTAATCTTGAAA AGCTATTACTCGTAGATTGTCCAAGGATGTTTAAAGATTGA

[0251] SEQ ID NO: 18: Amino acid sequence encoding Glyma.03G054100 conferring susceptible RIL

[0252] MSSFLLRFPPERYQEDNRNYDVFLSFRGDDTRASFTSHLYTALHNAGISVFKDDETLP

[0253] RGNKISTSLGLAIEESRLYVVVFSKNYAGSLWCLQELEKIMECHKATGQVVVPVFYD VDPSEVRHQTGHFGQAFRNLEAYINLKMEEEMQPGWQKMVHECPGISGPSVFRDCN GQSEILERIHRLVEDWRVSLRKIVSISPGSGFGKMDVVGNKIDNLVERWRDGLCVAT RIPWRGMLIAEQLIDLLVKHWRTTLRKIMEMPSNIDLLLKHWSEALWKAAGISGGAD LISKNEMTAPLDLAIHLLVKDWRKAHRAVRDISEGVVLKSIVEEEIRKDFEVLVIHWK EALHEAAGISRIRIIAEEFAKHWAEVLREAASISGIVVLNSRNESEAIKTIVENVKPLLD

[0254] KTELFVADNPVGVEPRVQEMIELLDQIQSNGVLLLGMWGMGGIGKTTIAKAIYNKIG RNFEVKSFLASIREVWGQDAGQVYLQEQLIFDIGKETNTKIRNVDSGKVMLKERLRN KRVLLILDDVNNLHQLNVLCGSREWFGSGSRIIITTRDMHILRGRRVDKVFRMKGMD EDESIELFSWHAFKQASPREDFIELSRNVVAYSAGLPLALEVLGKYLFDMEVTEWKN VLETLKKIPNDEVQEKLKISYDGLTGDTEKGIFLDIACFFTGKDRNDVIHILNGCGLCA ENGIRVL VERGE VTVDYKNKLGMHDLLRDMGREIIRSETPMELEERSRLWFHED AL

[0255] DVLSKETGTKAIEGLALKLPRTNTKCLSTKAFKEMKKLRLLQLAGVQLVGDFKYLS KDLRWLCWHGFPLACIPTNLYQGSLVSIELENSNVNLLWKEAQVMEKLKILNLSHSH YLTETPDFSNLPNLEKLLLVDCPRMFKD* SEQ ID NO: 19: Nucleotide sequence encoding Glyma.l0G285300 conferring susceptible

[0256] RIL

[0257] ATGTCTTCCGGCGGCGGCGGTGGTAAGCCGTTCTTCTGCCACGTGTGCAGTCAGA

[0258] GGATTACGTGCTCCGATGAGTCGGAACCATTCTGCCCGATGTGTATGGAAAGTTT

[0259] CGTAGAAGAATGCAACCCTAACAATCCCAACCCTAATCTCTTCCCCGACAGTGAT

[0260] GAATCTTCGGATCCCGAACTCCCCTTCCACCGCTTCTCACTTCTCCCTCTCTTGCT

[0261] CTCATCAGTCTCTAGGTCCCGATCCGAACCCGACGTGTTCGACCCGATGGTCTTC

[0262] CTCCAGAACCACCTTCAGGACCTTCGCGCCGACGGTGCCAACATTCAAGTTGACT

[0263] TTGACCATCCTTCCAACGAGAATCAAGGCTTTCGCCTTGCGAACATCGGCGACTA

[0264] CTTTATGGGCCCCGGCCTCGAGCAGTTTATTCAGCAGCTTGCCGACAACGACCCC

[0265] AACCGCTACGGAACGCCGCCCGCCGCCAAGGACGCCGTCGAGAATCTCCCCACC

[0266] GTTACCGTCGACGACGACTTGCTGAACTCCGAGCTGAACCAGTGCGCTGTCTGCC

[0267] AGGACGAGTTCGAAAAGGGTTCTAAGGTGACACAGATGCCCTGCAAGCACGCGT

[0268] ATCACGGCGACTGCTTGATTCCGTGGCTTCGGCTGCATAATTCATGCCCTGTGTG

[0269] CCGCTATGAATTGCCCACGGACGACGCCGATTACGAGAACGAGGTTCACGGTGG

[0270] TGATGCTGGGTCGAGGACAGGTGGGAGCGACGGTGGAGGTGGGAGTAATAGACC

[0271] TTTTCGCAGGACTGTTAGGATATATTTGCGCCATCCTGATGCTGGTGACTCTGCGC

[0272] AGGACGGTGCTGAAAGGGAATGGAGATGGAGAAGTTGA

[0273] SEQ ID NO: 20: Amino acid sequence encoding Glyma.l0G285300 conferring susceptible

[0274] RIL

[0275] MSSGGGGGKPFFCHVCSQRITCSDESEPFCPMCMESFVEECNPNNPNPNLFPDSDESS

[0276] DPELPFHRFSLLPLLLSSVSRSRSEPDVFDPMVFLQNHLQDLRADGANIQVDFDHPSN

[0277] ENQGFRLANIGDYFMGPGLEQFIQQLADNDPNRYGTPPAAKDAVENLPTVTVDDDL

[0278] LNSELNQCAVCQDEFEKGSKVTQMPCKHAYHGDCLIPWLRLHNSCPVCRYELPTDD

[0279] ADYENEVHGGDAGSRTGGSDGGGGSNRPFRRTVRIYLRHPDAGDSAQDGAEREWR WRS*

[0280] SEQ ID NO: 21: Nucleotide sequence encoding Glyma.04G225500 conferring susceptible

[0281] RIL ATGGCGCTGACGATGATGGCGGCGATCGGCATCGGCATGAAGCAAGAGAAGAA GATGCCGGCGACGCCGGCGCCGGAGAACGAGCTGAAGAAGCGGAATGAGGAGC TCGAGAAGGAGCTCAGAGAGAGCAAGGAGAGGGAGGAGCAGATGAAGCGCGAA

[0282] CTCCAGAGCGCGTGGGAGAGGCTGCGCGTGGCCGAGGAGGCTGAGGAGAGGCTC

[0283] TGCTCCCAGCTCGGAGAGCTAGAAGCAGAGGCCGTTTACCACGCGCGTGACTAC

[0284] CACGCGCGCATCGTCTCCCTCATGGACCAGCTCTCACGCGCCCAGAGCCTCCTCC TCAAGACCGGTGCCTCCTCCATTTCGCTTCCTTCCTCCTCCTAA

[0285] SEQ ID NO: 22: Amino acid sequence encoding Glyma.04G225500 conferring susceptible

[0286] RIL

[0287] MALTMMAAIGIGMKQEKKMPATPAPENELKKRNEELEKELRESKEREEQMKRELQS

[0288] AWERLRVAEEAEERLCSQLGELEAEAVYHARDYHARIVSLMDQLSRAQSLLLKTGA SSISLPSSS*

[0289] SEQ ID NO: 23: Nucleotide sequence encoding Glyma.03G253600 conferring resistant RIL

[0290] ATGTCCCTTAATTCATGCGGCTTTCAAAGCTTGTACCTTGGCAGTTCCAAATGCTC

[0291] CAACCTCGTTTTCCAGGATGTTAGGAAACTGCAACAGTACCAAGATGCATTAGAC

[0292] AAGTGCTCCAACTTCGACCACCCATTTGGTGAATCTTGTGCAGATTGCACCGGTG

[0293] CAATATTAAGCTTAAGAGATAGTTTATATAATCAAGTGACCAACAACAACAACA

[0294] ACCACACTGAGGTAGCCATATGTGCGGTAGCAGCTATTGTTGCTGTTGCAGCTGG

[0295] GAAACCAAATGATCCTGCCGTTGACAAAGTCTTACGCTGCTTGCCACCTTCAGCT

[0296] TCTGGATCAGACAAGAGATCATTGTGGAAATCCTTGTTGAGTGTGCCGGTAGTTA

[0297] TCCTTGCAATATTGCTTGTGGTTATAATGGTAAAACGTTTGTCCAAGAAGAAGCT

[0298] TCGTAGACAGGCAAACTTGAAAGAGATCGCTGCGTGGTCTGGATTGTACTGGTTC

[0299] TGCAAAAGGGAAATTGAGAATGCCATGAATTATGGCGGTGAAAAGATATGCCTT

[0300] GGACGTGGGAGTGCAGGGCAGGTGTATAGAGGTATTCTCCCAAGTGGTCAACTC

[0301] GTGGCCATCAAGCATTTAACAAAGAGTAACACCTCTGAGTCTTTCACTCGAGAAG

[0302] TTGAAGGTCTTTCAAGGCTTCGCCATCCTAACCTGGTTTGCCTCTTTGGATGCTGC

[0303] ATAGAAGGGGATGAGAGATATTTAGTCTATGAATTCTGTGCAAACGGGAATCTT

[0304] GCTCAACATCTCTTAAGAAGAGATAGCCACTTGACATGGGAAACCAGAGTAAGA

[0305] ATTTTGAGAGATTGTTCATATGCACTCAAGTACCTCCACCATCATATAGAAGGCT GTGTTGTCCATAGAGATATAAAGCTTACGAACATTCTTTTGAATGAGAAATACCA AGCGAAACTGTCAGATTTTGGACTGGCAAAAGTGATGGGGATTAAAGAGAGCAA GGTTTTTACTGATGTTAGAGGAACAATAGGCTACATGGATCCAGAGTACATGAGT AATGCCAAGCTAACCTGCGCTAGCGATGTTTACAGTTTTGGTATTGTTGCTCTAC AAATTCTGTCGGGACAGAAAGTCATCGAGTTGGATCTTGATGCCAGAGATCAACT CACTAGGAAGGCAAGAGATGTGAGCATGGGAAAGCGTCCACTGTCAGATTTTGA AGACCCGCGACTAAATGGAAAAGTTGATAAGACAGACTTCGAAGCCATCCTTCA GATTGCAGTTTTGTGTGTTGCCAAATCAAGCAAAGGTCGTCCAACCATTGAGCTT GTTTTTGAGGAATTGGACAAGGTCTGCAGGGATACAGAAACACGGATGAAGCAA AAGAAGGATGAGAGCTTATCAACAACATCCACCCCGAGCTCCAAATCGTCAAAA TCGGCGCCTCTATGA

[0306] SEQ ID NO: 24: Amino acid sequence encoding Glyma.03G253600 conferring resistant RIL MSLNSCGFQSLYLGSSKCSNLVFQDVRKLQQYQDALDKCSNFDHPFGESCADCTGAI LSLRDSLYNQVTNNNNNHTEVAICAVAAIVAVAAGKPNDPAVDKVLRCLPPSASGS DKRSLWKSLLSVPVVILAILLVVIMVKRLSKKKLRRQANLKEIAAWSGLYWFCKREI ENAMNYGGEKICLGRGSAGQVYRGILPSGQLVAIKHLTKSNTSESFTREVEGLSRLR HPNLVCLFGCCIEGDERYLVYEFCANGNLAQHLLRRDSHLTWETRVRILRDCSYALK YLHHHIEGCVVHRDIKLTNILLNEKYQAKLSDFGLAKVMGIKESKVFTDVRGTIGYM DPEYMSNAKLTCASDVYSFGIVALQILSGQKVIELDLDARDQLTRKARDVSMGKRPL SDFEDPRLNGKVDKTDFEAILQIAVLCVAKSSKGRPTIELVFEELDKVCRDTETRMKQ KKDESLSTTSTPSSKSSKSAPL*

[0307] SEQ ID NO: 25: Nucleotide sequence encoding Glyma.04Gl 85400 conferring resistant RIL ATGGATTCCATTTACATCTGTCATTTCATTCTACTACTGACCATAGTGTGCACTGT TGTTGTTGCAACTCTAGGAGATAACACAACAGAATCCTACTGGCTTCTAAGAATT AAATCAGAACTGGTTGATCCATTAGGAGCCCTGAGAAACTGGTCTCCAACAACA ACTCAAATTTGTAGCTGGAATGGACTAACATGTGCACTTGATCAGGCACGTGTTG TAGGCCTTAATTTGTCTGGCTCAGGACTATCAGGTTCCATTTCAGGAGAGTTCAG CCACCTCATTTCTCTTCAATCACTTGATTTGTCTTCAAACTCCCTCACAGGCTCAA TCCCTTCTGAACTTGGGAAGCTTCAAAATCTAAGAACACTTCTGCTTTACTCAAA TTATCTCTCTGGTGCCATTCCTAAAGAGATAGGTAATTTGAGCAAGTTGCAAGTT CTTAGACTAGGAGATAACATGTTGGAAGGTGAAATAACACCTAGTATTGGCAAC TTGAGTGAGTTGACAGTGTTTGGTGTAGCCAACTGCAACTTAAATGGAAGCATAC CTGTTGAGGTTGGTAAGTTGAAGAATCTTGTGTCTCTTGATTTGCAAGTGAACAG TCTTAGTGGTTACATACCTGAAGAGATTCAAGGCTGTGAAGGGCTCCAAAATTTT

[0308] GCAGCATCAAACAACATGCTTGAAGGAGAAATACCCTCCTCTTTGGGGTCTCTCA

[0309] AATCATTGAGAATTCTGAACCTGGCCAATAACACTCTATCAGGATCAATTCCTAC

[0310] CTCTTTGAGTCTTCTCTCCAATTTGACATACCTGAATTTGCTTGGAAACATGTTAA

[0311] ATGGTGAAATTCCTTCAGAGCTTAACAGTTTGAGCCAGCTACAGAAGCTTGACTT

[0312] ATCCAGAAACAGCCTTTCTGGACCACTAGCCCTCCTCAATGTCAAATTACAGAAT

[0313] CTTGAAACTATGGTTCTGTCTGATAATGCTTTAACAGGTAGTATTCCATATAACTT

[0314] CTGCCTCAGAGGTTCTAAACTTCAGCAACTGTTCTTAGCTAGGAATAAGCTTTCT

[0315] GGAAGATTTCCCTTGGAGCTTCTCAACTGCAGCTCAATCCAACAGGTGGACCTTT

[0316] CTGATAACAGTTTTGAAGGTGAACTTCCATCAAGCCTGGACAAGCTACAGAACCT

[0317] CACAGATCTTGTGCTCAACAACAACAGCTTCAGTGGATCTCTACCTCCAGGAATT

[0318] GGAAACATTAGTAGCTTGAGAAGCCTTTTCTTGTTTGGTAACTTTTTCACAGGAA

[0319] AACTCCCTGTGGAGATTGGAAGGCTTAAGAGGTTGAACACCATTTACCTCTATGA

[0320] TAACCAGATGTCTGGACCTATACCAAGAGAGTTAACAAACTGCACAAGATTAAC

[0321] TGAAATTGACTTCTTTGGAAACCATTTTTCTGGTCCCATTCCAAAGACTATAGGTA

[0322] AGCTAAAGGACTTAACTATTCTCCATTTAAGGCAAAATGATCTGTCAGGTCCAAT

[0323] CCCACCAAGCATGGGGTACTGTAAAAGGCTTCAGTTATTGGCCTTAGCAGATAAC

[0324] AAGTTGTCAGGTTCCATACCCCCCACATTCAGTTACCTTTCACAAATTAGAACCA

[0325] TTACCCTTTACAACAACTCCTTCGAAGGACCACTACCCGATTCCCTCTCTCTTCTT

[0326] AGAAACCTTAAAATCATAAACTTTTCCAATAACAAGTTCAGTGGAAGTATCTTTC

[0327] CTCTGACTGGTTCAAATTCTCTCACTGTTTTGGACTTGACAAACAACAGCTTCTCA

[0328] GGTTCCATCCCTTCTATTCTAGGCAACTCCAGAGATCTCACGCGTCTCAGACTTG

[0329] GAAACAATTATCTCACAGGAACCATTCCTTCTGAACTTGGCCACCTCACTGAGCT

[0330] AAACTTCCTTGATTTGTCATTCAACAACTTGACAGGACATGTGCTACCTCAACTCT

[0331] CAAACTGCAAGAAAATTGAACACCTTCTACTGAATAATAACAGATTGAGCGGGG

[0332] AAATGTCGCCTTGGCTGGGAAGCTTACAAGAACTTGGTGAGCTGGATCTCTCATT

[0333] CAACAACTTTCATGGAAGGGTTCCTCCTGAGCTTGGTGGCTGCTCAAAGTTGCTT

[0334] AAGCTTTTTCTCCATCACAACAATCTCTCGGGTGAAATCCCGCAAGAGATTGGAA

[0335] ACCTTACTTCACTCAATGTCTTCAACTTGCAAAAGAATGGTCTCTCTGGCCTCATT

[0336] CCATCAACAATTCAGCAATGCACCAAGCTGTATGAGATAAGGCTCTCAGAGAAC

[0337] TTCCTCTCAGGTACTATACCAGCTGAACTAGGAGGGGTTACTGAGTTACAAGTCA

[0338] TATTGGACTTGAGTAGAAACCACTTTTCTGGTGAGATTCCATCATCTCTTGGAAA

[0339] TCTCATGAAGCTAGAAAGACTTGATCTTTCCTTTAACCATCTTCAAGGACAAGTT CCTCCTTCACTTGGTCAACTTACCAGCCTGCATATGCTAAATCTCTCATATAACCA TCTTAATGGCCTCATTCCCTCAACCTTTTCAGGGTTCCCATTAAGCTCCTTTCTGA ACAATGACCACTTGTGTGGCCCTCCACTAACATTGTGCTTGGAAGCTACGGGTAA GGAGCGAATGCAGCTATCAAACGCACAAGTAGCAGCAATCATAGTAGCCATTGT CCTTACTTCCACCCTGATATGCTTAGTAATGTTGTATATAATGTTGAGAATCTGGT GCAACTGGATAAAGGTAGCTGTTTCAAGTGAAGATGGTGGCATGGTTGAGCAGC AGAAGACAAGAAATGGAGAGTACTGGAATATGAATTCTCCTGAGTTGTTTCCTTC ACCAGATAAACAAATTTCGCCAACAATTTGCATTTGCAGTCTCAAAATTGATGCA GAAGCCCAGGAAAATACCTTGGTCAGATAA

[0340] SEQ ID NO: 26: Amino acid sequence encoding Glyma.04Gl 85400 conferring resistant RIL MDSIYICHFILLLTIVCTVVVATLGDNTTESYWLLRIKSELVDPLGALRNWSPTTTQIC SWNGLTCALDQARVVGLNLSGSGLSGSISGEFSHLISLQSLDLSSNSLTGSIPSELGKL QNLRTLLLYSNYLSGAIPKEIGNLSKLQVLRLGDNMLEGEITPSIGNLSELTVFGVANC NLNGSIPVEVGKLKNLVSLDLQVNSLSGYIPEEIQGCEGLQNFAASNNMLEGEIPSSL GSLKSLRILNLANNTLSGSIPTSLSLLSNLTYLNLLGNMLNGEIPSELNSLSQLQKLDLS RNSLSGPLALLNVKLQNLETMVLSDNALTGSIPYNFCLRGSKLQQLFLARNKLSGRF PLELLNCSSIQQVDLSDNSFEGELPSSLDKLQNLTDLVLNNNSFSGSLPPGIGNISSLRS LFLFGNFFTGKLPVEIGRLKRLNTIYLYDNQMSGPIPRELTNCTRLTEIDFFGNHFSGPI PKTIGKLKDLTILHLRQNDLSGPIPPSMGYCKRLQLLALADNKLSGSIPPTFSYLSQIRT ITLYNNSFEGPLPDSLSLLRNLKIINFSNNKFSGSIFPLTGSNSLTVLDLTNNSFSGSIPSI LGNSRDLTRLRLGNNYLTGTIPSELGHLTELNFLDLSFNNLTGHVLPQLSNCKKIEHL LLNNNRLSGEMSPWLGSLQELGELDLSFNNFHGRVPPELGGCSKLLKLFLHHNNLSG EIPQEIGNLTSLNVFNLQKNGLSGLIPSTIQQCTKLYEIRLSENFLSGTIPAELGGVTELQ VILDLSRNHFSGEIPSSLGNLMKLERLDLSFNHLQGQVPPSLGQLTSLHMLNLSYNHL NGLIPSTFSGFPLSSFLNNDHLCGPPLTLCLEATGKERMQLSNAQVAAIIVAIVLTSTLI CLVMLYIMLRIWCNWIKVAVSSEDGGMVEQQKTRNGEYWNMNSPELFPSPDKQISP TICICSLKIDAEAQENTLVR*

[0341] SEQ ID NO: 27: Nucleotide sequence encoding Glyma.l8G244600 conferring resistant RIL ATGGGGTCTATGAATTTGTTAGGTTTTTCTCTCTCTCCTCAAGAACACCCTTCTAG TCAAGATCACTCTCAAACGGCACCTTCTCGTTTTTGCTTCAACCCTGATGGAATCT CAAGCACTGATGTAGCAGGAGACTGCTTTGATCTCACTTCTGACTCAACTCCTCA TTTACTCAACCTTCCCTCTTACGGCATATACGAAGCTTTTCATAGGAGCAACAAT

[0342] ATTCACACCACTCAAGATTGGAAGGAGAACTACAACAGCCAAAACTTGCTATTG

[0343] GGAACTTCATGCAGCAACCAAAACATGAACCACAACCATCAGCAACAACAACAA

[0344] CAACAGCCAAAGCTTGAAAACTTCCTCGGTGGACACTCATTTGGTGAACATGAGC

[0345] AACCCTACGGTGGTAACTCAGCCTCTACAGAATACATGTTCCCGGCTCAGCCGGT

[0346] ATTGGCCGGTGGCGGCGGCGGTGGTAGCAATAGCAGCAACACAAGCAACAGTAG

[0347] CTCCATAGGGTTATCCATGATAAAGACATGGTTGAGGAACCAACCACCACACTC

[0348] AGAAAACAACAATAACAACAACAATGAAAGTGGTGGCAATAGTAGAAGCAGTG

[0349] TGCAGCAGACTCTATCACTTTCCATGAGTACTGGTTCACAATCAAGCACATCACT

[0350] ACCCCTTCTCACTGCTAGTGTGGATAATGGAGAGAGTTCTTCTGATAACAAACAA

[0351] CCACATACCACGGCTGCACTTGATACAACCCAAACCGGAGCCATTGAAACTGCA

[0352] CCCAGAAAGTCCATTGACACTTTTGGACAGAGAACTTCTATCTACCGTGGTGTAA

[0353] CAAGGCATAGGTGGACGGGGAGGTATGAGGCTCACCTGTGGGATAATAGTTGTA

[0354] GAAGAGAGGGACAAACTCGCAAAGGAAGGCAAGTTTACTTGGGAGGTTATGACA

[0355] AAGAAGAAAAGGCAGCTAGAGCCTACGATTTGGCAGCACTAAAATACTGGGGAA

[0356] CAACTACGACAACAAATTTTCCAATTAGCCACTATGAGAAAGAGTTGGAAGAAA

[0357] TGAAGCACATGACTAGGCAAGAGTACGTTGCGTCATTGAGAAGGAAGAGTAGTG

[0358] GGTTTTCTCGCGGGGCATCCATTTATCGAGGTGTGACGAGACACCATCAACATGG

[0359] AAGATGGCAAGCGAGGATTGGAAGAGTTGCTGGCAACAAGGATCTCTACTTGGG

[0360] AACTTTCAGCACCCAAGAGGAGGCAGCAGAAGCATATGATGTAGCAGCAATCAA

[0361] ATTCAGAGGACTAAGTGCTGTTACAAACTTTGACATGAGCAGATATGACGTGAA

[0362] AAGCATACTTGAGAGCACCACTTTGCCAATTGGTGGTGCTGCAAAGCGTTTGAAG

[0363] GATATGGAGCAGGTGGAACTGAGGGTGGAGAATGTTCATAGAGCAGATCAAGAA

[0364] GATCATAGTAGCATCATGAACTCTCACTTAACTCAAGGAATCATTAACAACTATG

[0365] CAGCAGGAGGAACAACAGCGACTCATCATCATAACTGGCACAATGCTCTTGCAT

[0366] TCCACCAACCTCAACCTTGCACCACCATACACTACCCTTATGGACAAAGAATTAA

[0367] TTGGTGCAAGCAAGAACAAGACAACTCTGATGCCTCTCACTCTTTGTCTTATTCA

[0368] GATATTCATCAACTACAGCTAGGGAACAATGGCACACACAACTTCTTTCACACAA

[0369] ATTCAGGGTTGCACCCTATGTTAAGCATGGATTCTGCTTCCATTGACAATAGCTCT

[0370] TCATCTAACTCTGTTGTTTATGATGGTTATGGAGGTGGTGGGGGCTATAATGTGA

[0371] TTCCTATGGGGACTACTACTACTGTTGTTGCAAATGATGGTGATCAAAATCCAAG

[0372] AAGCAATCATGGTTTTGGTGATAATGAGATAAAGGCACTTGGTTATGAAAGTGTG

[0373] TATGGTTCTACAACTGATCCTTATCATGCACATGCAAGGAACTTGTATTATCTTAC TCAACAGCAACCATCTTCTGTTGATGCAGTGAAGGCTAGTGCATATGATCAAGGA

[0374] TCTGCATGCAATACTTGGGTTCCAACTGCTATTCCAACTCATGCACCAAGGTCTA

[0375] GTACTAGTATGGCTCTCTGCCATGGTGCTACGCCCTTCTCTTTATTGCATGAATAG

[0376] SEQ ID NO: 28: Amino acid sequence encoding Glyma.l8G244600 conferring resistant RIL MGSMNLLGFSLSPQEHPSSQDHSQTAPSRFCFNPDGISSTDVAGDCFDLTSDSTPHLL NLPSYGIYEAFHRSNNIHTTQDWKENYNSQNLLLGTSCSNQNMNHNHQQQQQQQPK LENFLGGHSFGEHEQP YGGNS ASTEYMFP AQPVL AGGGGGGSNS SNTSNS S SIGLSMI KTWLRNQPPHSENNNNNNNESGGNSRSSVQQTLSLSMSTGSQSSTSLPLLTASVDNG ESSSDNKQPHTTAALDTTQTGAIETAPRKSIDTFGQRTSIYRGVTRHRWTGRYEAHL WDNSCRREGQTRKGRQVYLGGYDKEEKAARAYDLAALKYWGTTTTTNFPISHYEK ELEEMKHMTRQEYVASLRRKS SGF SRGASIYRGVTRHHQHGRWQARIGRVAGNKD LYLGTFSTQEEAAEAYDVAAIKFRGLSAVTNFDMSRYDVKSILESTTLPIGGAAKRLK DMEQVELRVENVHRADQEDHSSIMNSHLTQGIINNYAAGGTTATHHHNWHNALAF HQPQPCTTIHYPYGQRINWCKQEQDNSDASHSLSYSDIHQLQLGNNGTHNFFHTNSG LHPMLSMDS ASIDNS S S SNS VVYDGYGGGGGYNVIPMGTTTT VVANDGDQNPRSNH GFGDNEIKALGYESVYGSTTDPYHAHARNLYYLTQQQPSSVDAVKASAYDQGSACN TWVPTAIPTHAPRS STSMALCHGATPF SLLHE*

[0377] SEQ ID NO: 29: Nucleotide sequence encoding Glyma. l2G216900 conferring susceptible RIL

[0378] ATGGCTGTCAAAGTTTATATTGTGTACTACTCCATGTATGGGCATGTTGAGAAAC TAGCAGAAGAAATAAAGAAAGGGGCTTCCTCTGTGGAAGGTGTTGAGGCCAAAT TATGGCAGGTACCTGAGACGCTGCAGGATGAGGTGCTCGGTAAGATGAGTGCAC CACCCAAGAGCGATGTACCAGTCATTACCCCGAATGAACTATCCGAGGCTGATG GCTTTGTATTCGGCTTCCCAACAAGGTTTGGAATGATGGCTGCTCAGTTTAAAGC TTTTCTAGATGCTACTGGAGGCTTATGGAGAGCACAACAGCTTGCAGGCAAGCCT GCTGGCATCTTCTACAGCACTGGTTCACAAGGCGGCGGACAAGAGACTACAGCG CTCACTGCTATCACTCAACTGGTTCATCATGGGATGATATTCATTCCAATCGGTTA CACGTTTGGTGCTGGCATGTTCGAGATGGAGAAAGTGAAAGGTGGAAGTCCATA TGGTGCCGGAACTTATGCCGGTGACGGCTCAAGACAGCCAAGTGAGCTCGAGTT ACAGCAAGCATTCCACCAAGGCAAGTACATTGCCGGCATCACAAAGAAGCTCAA GCAAGCTGCATAA SEQ ID NO: 30: Amino acid sequence encoding Glyma.l2G216900 conferring susceptible RIL MAVKVYIVYYSMYGHVEKLAEEIKKGASSVEGVEAKLWQVPETLQDEVLGKMSAP PKSDVPVITPNELSEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRAQQLAGKPAG IFYSTGSQGGGQETTALTAITQLVHHGMIFIPIGYTFGAGMFEMEKVKGGSPYGAGTY AGDGSRQPSELELQQAFHQGKYIAGITKKLKQAA*

[0379] SEQ ID NO: 31: Nucleotide sequence encoding Glyma.U032805 conferring susceptible RIL ATGGATTCACGAGATCTAGGAACTCAACTCCAAAACCGGGTGGCAACACCCCTC CTCATAACTCCAGCTACCACTGTGAACTCGAGCGAAAGCAAGGGTTACCTCGTCA GCAAAGGGAAGCTTGATCTTGTGGGGATCGAGGAACCACACGAGGTTCTTGTTG ACATAATTGAGCGGATGATGGAATCTTCGAGGATGACATTGTTTTGGAGCATCGA AGCTCCAGATCTAGATCTGGTGAAGCACTCTGAAGCTCCAGACAATATTTAG

[0380] SEQ ID NO: 32: Amino acid sequence encoding Glyma.U032805 conferring susceptible

[0381] RIL

[0382] MDSRDLGTQLQNRVATPLLITPATTVNSSESKGYLVSKGKLDLVGIEEPHEVLVDIIE RMMESSRMTLFWSIEAPDLDLVKHSEAPDNI*

[0383] Accordingly, certain embodiments of the invention provide a method of producing an SCN resistant plant cell or a plant comprising introducing one or more genes comprising a protein coding sequence selected from SEQ ID NOs: 5-8, SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27 into the plant. Further embodiments of the invention provide a method of producing an SCN resistant plant cell or a plant comprising overexpressing in the plant cell or the plant a gene encoding one or more genes comprising a protein coding sequence selected from SEQ ID NOs: 5-8, SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27. In preferred embodiments, the plant cell or a plant is a soybean plant cell or soybean plant.

[0384] As used herein, the term “overexpressing a gene” or grammatical variations thereof refer to a condition in a genetically modified plant cell or a genetically modified plant wherein the gene encodes for a protein at a level higher than the parent plant cell or the plant without the genetic modification. Thus, a parent plant cell or a parent plant is genetically modified to produce a modified plant cell or modified plant that expresses a gene to produce a protein at a higher level compared to the parent plant cell or parent plant.

[0385] Typically, overexpressing a gene in a plant cell or a plant comprises introducing into the plant cell or a plant, a nucleic acid construct comprising the gene (operably linked to a endogenous or exogenous promoter and, optionally, other control elements (e.g., an enhancer)). The nucleic acid construct is designed to induce the expression of the protein encoded by the gene. Methods of producing and introducing various nucleic acid constructs comprising genes of interest into a plant cell or a plant to overexpress the genes are well known to a person of ordinary skill in the art and such embodiments are within the purview of the invention. Certain such embodiments are identified below.

[0386] A gene is referred to as “operably linked” when it is placed into a functional relationship with another DNA segment (for example, a promoter that is operably linked to any one of SEQ ID NOs: 5-8, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, or SEQ ID NO: 31). In certain embodiments, the genes are operably linked to exogenous promoters (any promoter not naturally associated with the gene to which it is operably linked). However, enhancers need not be contiguous with the coding sequences whose transcription they control. Linking is accomplished by ligation at convenient restriction sites or at adapters or linkers inserted in lieu thereof. The expression cassette can include one or more enhancers in addition to the promoter. By “enhancer” is intended a cv.s-acting sequence that increases the utilization of a promoter. Such enhancers can be native to a gene or from a heterologous gene. Further, it is recognized that some promoters can contain one or more native, enhancers or enhancerlike elements. An example of one such enhancer is the 35S enhancer, which can be a single enhancer, or duplicated. See for example, McPherson et al, U.S. Pat. No. 5,322,938, which is hereby incorporated by reference in its entirety.

[0387] The promoter for driving expression of the genes of interest may be selected based on a number of criteria including, but not limited to, what the desired use is for the operably linked polynucleotide, what location in the plant is expression of the gene of interest desired, and at what level is expression of gene of interest desired or whether it needs to be controlled in another spatial or temporal manner. In one aspect, a promoter that directs expression to particular tissue may be desirable. When referring to a promoter that directs expression to a particular tissue is meant to include promoters referred to as tissue specific or tissue preferred. Included within the scope of the invention are promoters that express highly in the plant tissue, express more in the plant tissue than in other plant tissue, or express exclusively in the plant tissue. For example, “seed-specific” promoters may be employed to drive expression. Specific-seed promoters include those promoters active during seed development, promoters active during seed germination, and / or that are expressed only in the seed. Seed-specific promoters, such as annexin, P34, beta-phaseolin, alpha subunit of beta-conglycinin, oleosin, zein, napin promoters have been identified in many plant species such as maize, wheat, rice and barley. See U.S. Pat. Nos. 7,157,629, 7,129,089, and 7,109,392. Such seed-preferred promoters further include, but are not limited to, Ciml (cytokinin-induced message); cZ19Bl (maize 19 kDa zein); and milps (myo-inositol- 1 -phosphate synthase); (see WO 00 / 11177, herein incorporated by reference). The 27 kDa gamma-zein promoter is a preferred endospermspecific promoter. The maize globulin- 1 and oleosin promoters are preferred embryo-specific promoters. For dicots, seed-specific promoters include, but are not limited to, bean beta phaseolin, napin, beta-conglycinin, soybean lectin, cruciferin, and the like. For monocots, seed-specific promoters include, but are not limited to, promoters of the 15 kDa beta-zein, 22 kDa alpha-zein, 27 kDa gamma-zein, waxy, shrunken 1, shrunken 2, globulin 1, an Ltpl, an Ltp2, and oleosin genes. See also WO 00 / 12733, where seed-preferred promoters from endl and end2 genes are disclosed; herein incorporated by reference. Each of these aforementioned references is hereby incorporated by reference in its entirety, particularly as it relates to the promoters disclosed within the reference.

[0388] In preferred embodiments, a promoter used in the present invention is a soybean ubiquitin promoter, for example, the promoters for soybean ubiquitin B (UBB) / ubiquitin C (UBC) gene. Certain examples of soybean ubiquitin promoters that could be used in the present invention are described in United States patent application publication numbers 20140053296 and 20100186119. Each of these publications is incorporated by reference in its entirety, particularly, the sequence listing. These promoters are considered exogenous promoters when operably linked to any one of the nucleic acid sequences disclosed herein.

[0389] The promoters useful in the present invention can also include constitutive, inducible or tissue-specific (preferred) promoters that are operably linked to a gene comprising a protein coding sequence of any one of SEQ ID NOs: 5-8, SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27 and are heterologous to the nucleic acid sequences to which they are operably linked. In other words, the promoters are not those found operably linked to a gene comprising a protein coding sequence of SEQ ID NOs: 5-8, SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27 in their native context within a plant, such as a soybean plant. Constitutive promoters, generally, are active in most or all tissues of a plant; inducible promoters, which generally are inactive or exhibit a low basal level of expression, and can be induced to a relatively high activity upon contact of cells with an appropriate inducing agent; tissue-specific (or tissuepreferred) promoters, which generally are expressed in only one or a few particular cell types (e.g., root cells); and developmental-or stage-specific promoters, which are active only during a defined period during the growth or development of a plant. Often promoters can be modified, if necessary, to vary the expression level. Certain embodiments comprise promoters exogenous to the species being manipulated (e.g. a soybean plant).

[0390] Non-limiting examples of root-specific promoters (a subset of tissue-specific promoters) include root preferred promoters, such as the maize NAS2 promoter, the maize Cyclo promoter (US 2006 / 0156439, published Jul. 13, 2006), the maize ROOTMET2 promoter (WO05063998, published Jul. 14, 2005), the CR1BIO promoter (WO06055487, published May 26, 2006), the CRWAQ81 (W005035770, published Apr. 21, 2005) and the maize ZRP2.47 promoter (NCBI accession number: U38790; GI No. 1063664). Each of these aforementioned references is hereby incorporated by reference in its entirety, particularly as it relates to the promoters disclosed within the reference.

[0391] Exemplary constitutive promoters include the 35S cauliflower mosaic virus (CaMV) promoter (Odell et al. (1985) Nature 313:810-812), the maize ubiquitin promoter (Christensen etal. (1989) Plant Mol. Biol. 12:619-632 and Christensen etal. (1992) PlantMol. Biol. 18:675- 689); the core promoter of the Rsyn7 promoter and other constitutive promoters disclosed in WO 99 / 43838 and U.S. Pat. No. 6,072,050; rice actin (McElroy et al. (1990) Plant Cell 2: 163- 171); pEMU (Last et al. (1991) Theor. Appl. Genet. 81 :581-588); MAS (Velten et al. (1984) EMBO J. 3:2723-2730); ALS promoter (U.S. Pat. No. 5,659,026); rice actin promoter (U.S. Pat. No. 5,641,876; WO 00 / 70067), maize histone promoter (Brignon et al., Plant Mol Bio 22(6): 1007-1015 (1993); Rasco-Gaunt et al., Plant Cell Rep. 21(6):569-576 (2003)) and the like. Other constitutive promoters include, for example, those described in U.S. Pat. Nos. 5,608,144 and 6,177,611, and PCT publication WO 03 / 102198. Each of these aforementioned references is hereby incorporated by reference in its entirety, particularly as it relates to the promoters disclosed within the reference.

[0392] An inducible promoter / regulatory element is one that is capable of directly or indirectly activating transcription of a gene comprising a protein coding sequence of one or more of SEQ ID NOs: 5-8, SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27 in response to an inducer. The inducer can be a chemical agent such as a protein, metabolite, growth regulator, herbicide or phenolic compound; or a physiological stress, such as that imposed directly by heat, cold, salt, or toxic elements, or indirectly through the action of a pathogen or disease agent such as a virus; or other biological or physical agent or environmental condition. A plant cell containing an inducible promoter / regulatory element may be exposed to an inducer by externally applying the inducer to the cell or plant such as by spraying, watering, heating or similar methods. An inducing agent useful for inducing expression from an inducible promoter is selected based on the particular inducible regulatory element. In response to exposure to an inducing agent, transcription from the inducible regulatory element generally is initiated de novo or is increased above a basal or constitutive level of expression.

[0393] Any inducible promoter / regulatory element can be used in the instant invention (See Ward et al., Plant Mol. Biol. 22: 361-366, 1993). Non-limiting examples of such promoters / regulatory elements include: a metallothionein regulatory element, a copper- inducible regulatory element, or a tetracycline-inducible regulatory element, the transcription from which can be effected in response to divalent metal ions, copper or tetracycline, respectively (Furst et al., Cell 55:705-717, 1988; Mett et al., Proc. Natl. Acad. Sci., USA 90:4567- 4571, 1993; Gatz et al., Plant J. 2:397-404, 1992; Roder et al., Mol. Gen. Genet. 243:32-38, 1994). Inducible promoters / regulatory elements also include an ecdysone regulatory element or a glucocorticoid regulatory element, the transcription from which can be effected in response to ecdysone or other steroid (Christopherson et al., Proc. Natl. Acad. Sci., USA 89:6314-6318, 1992; Schena c / a / ., Proc. Natl. Acad. Sci., USA 88: 10421-10425, 1991; U.S. Pat. No. 6,504,082); a cold responsive regulatory element or a heat shock regulatory element, the transcription of which can be effected in response to exposure to cold or heat, respectively (Takahashi et al., Plant Physiol. 99:383-390, 1992); the promoter of the alcohol dehydrogenase gene (Gerlach et al., PNAS USA 79:2981-2985 (1982); Walker et al., PNAS 84(19):6624-6628 (1987)), inducible by anaerobic conditions; and the light-inducible promoter derived from the pea rbcS gene or pea psaDb gene (Yamamoto etal. (1997) Plant J. 12(2):255- 265); a light-inducible regulatory element (Feinbaum et al., Mol. Gen. Genet. 226:449, 1991; Lam and Chua, Science 248:471, 1990; Matsuoka et al. (1993) Proc. Natl. Acad. Sci. USA 90(20):9586-9590; Orozco et al. (1993) Plant Mol. Bio. 23(6): 1129-1138), a plant hormone inducible regulatory element (Yamaguchi-Shinozaki et al., Plant Mol. Biol. 15:905, 1990; Kares et al., Plant Mol. Biol. 15:225, 1990), and the like. An inducible promoter / regulatory element also can be the promoter of the maize In2-1 or In2-2 gene, which responds to benzenesulfonamide herbicide safeners (Hershey et al., Mol. Gen. Gene. 227:229-237, 1991; Gatz etal., Mol. Gen. Genet. 243:32-38, 1994), and the Tet repressor of transposon TnlO (Gatz et aP Mol. Gen. Genet. 227:229-237, 1991). Stress inducible promoters include salt / water stress-inducible promoters such as P5CS (Zang et al. (1997) Plant Sciences 129:81-89); coldinducible promoters, such as, cor 15a (Hajela et al. (1990) Plant Physiol. 93: 1246-1252), corl5b (Wilhelm et al. (1993) Plant Mol Biol 23: 1073-1077), wscl20 (Ouellet et al. (1998) FEBS Lett. 423-324-328), ci7 (Kirch et al. (1997) Plant Mol Biol. 33:897-909), ci21A (Schneider eta / . (1997) Plant Physiol. 113:335-45); drought-inducible promoters, such as, Trg- 31 (Chaudhary et al (1996) Plant Mol. Biol. 30: 1247- 57), rd29 (Kasuga et al. (1999) Nature Biotechnology 18:287-291); osmotic inducible promoters, such as Rabl7 (Vilardell et al. (1991) Plant Mol. Biol. 17:985-93) and osmotin (Raghothama et al. (1993) Plant Mol Biol 23: 1117-28); and heat inducible promoters, such as heat shock proteins (Barros et al. (1992) Plant Mol. 19:665-75; Marrs etal. (1993) Dev. Genet. 14:27-41), smHSP (Waters etal. (1996) J. Experimental Botany 47:325-338), and the heat-shock inducible element from the parsley ubiquitin promoter (WO 03 / 102198). Other stress-inducible promoters include rip2 (U.S. Pat. No. 5,332,808 and U.S. Publication No. 2003 / 0217393) and rd29a (Yamaguchi-Shinozaki et al. (1993) Mol. Gen. Genetics 236:331-340). Certain promoters are inducible by wounding, including the Agrobacterium pmas promoter (Guevara-Garcia et al. (1993) Plant J. 4(3):495- 505) and the Agrobacterium ORF13 promoter (Hansen et al.. (1997) Mol. Gen. Genet. 254(3):337-343). Each of these aforementioned references is hereby incorporated by reference in its entirety, particularly as it relates to the promoters disclosed within the reference.

[0394] Overexpression of a gene comprising a protein coding sequence selected from SEQ ID NOs: 5-8, SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27 can also be achieved by one or one or more mutations in the endogenous promoter of the gene, wherein the one or more mutations increase the expression of the gene. For a particular gene, a skilled artisan can identify one or more mutations that would increase the expression of the gene and such embodiments are within the purview of the invention.

[0395] Certain preferred embodiments of the invention provide a method of producing a plant cell or a plant that is resistant to SCN, the method comprising overexpressing in the plant cell or the plant a gene comprising a protein coding sequence of a protein disulfide isomerase-like (PDIL). The overexpressed gene encoding the sulfite exporter PDIL protein can comprise a protein coding sequence of Glyma.l4G050600 (SEQ ID NO: 5).

[0396] Additional embodiments of the invention also provide a plant cell comprising an overexpressed gene comprising a protein coding sequence selected from SEQ ID NOs: 6-8, 24, 26, or 28. The plant cell or the plant can be a soybean plant cell or soybean plant. The overexpressed gene can comprise a protein coding sequence of Glyma. l4G050700 (SEQ ID NO: 6). The plant cell or a plant can also comprise overexpression of a gene encoding Glyma.14G056000.2 (SEQ ID NO: 7) or overexpression of a gene encoding Glyma.16G137000 (SEQ ID NO: 8), SEQ ID NO: 24, SEQ ID NO: 26, or SEQ ID NO: 28.

[0397] Further embodiments of the invention provide a method of producing an SCN resistant plant cell or a plant comprising inactivating in the plant one or more genes comprising a protein coding sequence selected from SEQ ID NOs: 1-4, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 29, or SEQ ID NO: 31. In preferred embodiments, the plant cell or a plant is a soybean plant cell or soybean plant.

[0398] Typically, inactivating a gene in a plant cell or a plant comprises introducing into the gene one or more mutations that inhibit or abolish the expression of an active protein from the gene. Mutations in a gene that inhibit or abolish the expression of a protein from the gene can be achieved either by deleting the entire coding region of the gene or a portion of the coding region of the gene, by introducing a frame shift mutation within the coding region of the gene, by introducing a missense mutation, insertion of sequences that disrupt the activity of the protein encoded by the gene, by introducing a stop codon or any combination of the aforementioned gene mutations. Inactivating a gene can also be performed by using molecular markers or other traditional breeding methods to integrate activated or inhibited genes in any soybean germplasm. Further, overexpressing one or more genes can be performed by introducing and / or expressing the one or more genes under soybean endogenous promoters and / or any exogenous promoters.

[0399] Methods of inactivating a gene of interest in a plant cell or a plant to inhibit or abolish the expression of an active protein from the gene are well known to a person of ordinary skill in the art and such embodiments are within the purview of the invention. Certain such embodiments are identified below.

[0400] Sanagala et al. (2017), Journal of Genetic Engineering and Biotechnology; 15(2):317- 321, describe several methods of inactivating a gene, for example, by implementing homologous recombination, zinc finger nucleases, Transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeats / CRISPR-associated (CRISPR / Q / .s) system. The Sanagala et al. reference is incorporated herein by reference in its entirety.

[0401] In preferred embodiments, inactivating a gene of interest is performed using the CRISPR / Q / .s system. An example of such system to inactivate genes in a plant cell or a plant is provided by Ordon et al. (2017), The Plant Journal; 89: 155-168. The Ordon et al. reference is incorporated herein by reference in its entirety.

[0402] Typically, a CRISPR / / .s system mediated inactivation of a gene involves the use of a guide RNA targeted to a gene of interest. A DNA oligomer targeted to a gene of interest can be transcribed into single guide RNA (sgRNA). sgRNA guides the Cas9 DNA endonuclease to the gene of interest by sgRNA hybridization to the target site. The endonuclease Cas9 makes a double strand break 3 bp upstream of Palindromic Adjacent Motif (PAM). The DNA breakage engages the repair mechanism, such as homologous recombination (HR) or the non- homologous end joining (NHEJ) mechanism. The NHEJ mechanism is a major double strand break repair pathway in plants and is known to be error prone. NHEJ DNA repair process introduces errors in the DNA repair, which causes irreversible mutations at the gene of interest. The chances of errors in DNA repair can be increased by providing multiple sgRNA. Based on the sequence of a gene comprising a protein coding sequence selected from SEQ ID NOs: 1-4, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 29 or SEQ ID NO: 31, a person of ordinary skill in the art can design and perform inactivation of the gene using the CRISPR / Q / .s system and such embodiments are within the purview of the invention.

[0403] Methods of inactivating a gene of interest in a plant cell or a plant to inhibit or abolish the expression of an active protein from the gene also include introduction into the plant cell or the plant one or more inhibitory oligonucleotides, such as small interfering RNA (siRNA) or short hairpin RNAs (shRNA). Methods of producing and introducing inhibitory RNA are also well known to a person of ordinary skill in the art and such embodiments are within the purview of the invention.

[0404] Certain preferred embodiments of the invention provide a method of producing a plant cell or a plant that is resistant to SCN, the method comprising inactivating in the plant cell or the plant a gene encoding a protein coding sequence of Glyma. l4G050600 (SEQ ID NO: 1), Glyma.14G050700 (SEQ ID NO: 2), Glyma.l4G056000.2 (SEQ ID NO: 3), or Glyma.16G137000 (SEQ ID NO: 4), SEQ ID NO: 17 (Glyma.03G054100), SEQ ID NO: 19 (Glyma.10G285300), SEQ ID NO: 21 (Glyma.04G225500), SEQ ID NO: 29 (Glyma.12G216900) or SEQ ID NO: 31 (Glyma.U032805).

[0405] In the methods of producing a plant cell described herein the plant cell can be in a plant part, for example, a seed, endosperm, ovule or pollen. The plant can be a soybean plant.

[0406] In preferred embodiments, an overexpressed gene encodes a protein coding sequence selected from SEQ ID NOs: 5-8, SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27. In preferred embodiments, an modified gene encodes a protein coding sequence selected from SEQ ID NOs: 5-8, SEQ ID NO: 23, SEQ ID NO: 25, or SEQ ID NO: 27. In preferred embodiments, an inactivated gene encodes a protein coding sequence selected from SEQ ID NOs: 1-4, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 29 or SEQ ID NO: 31.

[0407] The plant cell or the plant can be a soybean plant cell or a soybean plant.

[0408] The details discussed above for overexpressing, modifying, and inactivating genes in a plant cell or a plant are also applicable to the methods of identifying a gene that induces SCN resistance in a plant cell or a plant when overexpressed, modified, or inactivated and such embodiments are within the purview of the invention.

[0409] The methods disclosed herein for producing an SCN resistant plant includes editing a genome to replace one or more nucleotides in an SCN susceptible plant cell or a plant with one or more nucleotides that confer resistant to an SCN infection.

[0410] Several techniques for editing a genome to replace one or more nucleotide with different one or more nucleotides are known in the art and are within the purview of the invention. Such techniques include homologous recombination using sequences that flank a genomic site to replace a native sequence with a non-native sequence. Additional such techniques include genome editing using CRISPR-Cas9 system, Zinc Finger based nuclease (ZFN) system, Transcription Activator-Like Effector Nucleases (TALEN) system, viral systems, such as recombinant adeno-associated viruses (rAAV), and transposons. Details of using these systems are known in the art and a person of ordinary skill in the art can design appropriate machinery to edit a genome and such embodiments are within the purview of this invention. Additional techniques of genome editing are also known in the art and such embodiments are within the purview of the invention.

[0411] In certain embodiments, the methods of the subject invention further pertain to functional analysis assays of proteins using a protein that confers susceptibility to soybean cyst nematode, such as, for example, a protein that comprises a sequence selected from SEQ ID NOs: 9 to 12, 18, 20, 22, 30 and / or 32, as a negative control or a protein that confers resistance to soybean cyst nematode, such as, for example, a protein comprises a sequence selected from SEQ ID NOs: 13 to 16, 24, 26 and / or 28, as a positive control. In certain embodiments, the protein is assayed for its functional ability to confer resistance to soybean cyst nematode in an in vitro assay. In certain embodiments, a protein that confers susceptibility to soybean cyst nematode, such as, for example, a protein that comprises a sequence selected from SEQ ID NOs: 9 to 12, 18, 20, 22, 30 and / or 32, or a protein that confers resistance to soybean cyst nematode, such as, for example, a protein comprises a sequence selected from SEQ ID NOs: 13 to 16, 24, 26 and / or 28, can be used to raise antibodies specific to a protein selected from SEQ ID NOs: 9-16, 18, 20, 22, 24, 26, 28, 30, and / or 32. In certain embodiments, the raised antibodies can be used to detect the presence and / or cellular location of a protein that confers resistance or susceptibility to soybean cyst nematode (e.g. SEQ ID NOs: 9-16, 18, 20, 22, 24, 26, 28, 30, and / or 32).

[0412] Exemplary embodiments of such methods are described in Examples 1 to 6 below. A skilled artisan can implement such methods to identify genes that confer a trait of interest in a plant and such embodiments are within the purview of the invention.

[0413] MATERIALS AND METHODS

[0414] Developing Fs-Derived Riis Segregating For Resistance To Sen Hg Type 1.2.5.7.

[0415] A population of 114 Fs-derived RILs from a cross between TN09-029 and NCC05- 1168. The parental line TN09-029, a late maturity group IV line, was developed by a cross between Fowler and Anand (Anand et al., 2001; Young, 2001). TN09-029 is highly resistant to SCN Hg Type 1.2.5.7, Hg Type 0, and Hg Type 2.5.7 (Gillen and Shelton, 2011). Resistance to multiple SCN Hg types in TN09-029 is derived from PI 437654. The parental line NCC05- 1168 is an early maturity group V and was developed from a cross between TN97-167 and S99-2281. NCC05-1168 is resistant to SCN Hg Type 0 but susceptible to SCN Hg Type 1.2.5.7 and Hg Type 2.5.7 (Gillen and Shelton, 2011). NCC05-1168 resistance to Hg Type 0 is derived from Manokin (PI 559932). The RIL population was advanced through single pod descent until the Fs generation. The RILs were evaluated for resistance to SCN Hg Type 1.2.5.7 and Hg Type 0 in replicated greenhouse bioassays. The number of cysts per plants we counted and used to determine cyst index, which was calculated by dividing the average number of cysts on RILs by the average number of cysts determined on susceptible check multiplied by 100. Notably, all the 115 RILs exhibited resistant responses to Hg Type 0 with cyst index ranging between 0 and 9. As expected, the parental lines TN09-029 and NCC05-1168 were also resistant with cyst index of 0 and 4, respectively. In contrast to Hg Type 0, the RIL population segregated for resistance to HG type 1.2.5.7 (Figure 1A). More specifically, 49 RILs scored female index less than 10 and were reconsidered as resistant, while 65 RILs scored female index more than 10 and were considered as susceptible. This distribution is consistent with our finding that the parental line TN09-029 was resistant (female index = 0.5), whereas NCC05- 1168 was susceptible (female index = 95) to SCN HG type 1.2.5.7.

[0416] All patents, patent applications, provisional applications, and publications referred to or cited herein are incorporated by reference in their entirety, including all figures and tables, to the extent they are not inconsistent with the explicit teachings of this specification.

[0417] Following are examples which illustrate procedures for practicing the invention. These examples should not be construed as limiting. All percentages are by weight and all solvent mixture proportions are by volume unless otherwise noted.

[0418] EXAMPLE 1— RESISTANCE TO HG TYPE 1.2.5.7 IS NOT CONTROLLED BY THE Rhgl AND Rhg4 RESISTANCE GENES

[0419] We examined whether the SCN resistance genes Rhgl and Rhg4 are involved in the observed phenotypic variability to Hg Type 1.2.5.7. We first analyzed the coding sequence and promoter region of GmSNAP18 and GmSHMT08. the main genes at the Rhgl and Rhg4 locus, respectively, in various resistant and susceptible RILs of our population. Both resistant and susceptible lines contain the resistant alleles of GmSNAP18 and GmSHMT08 similar to that of Peking-type. Second, we investigated if copy number of Rhgl and Rhg4 loci are varied among the resistant and susceptible RILs. To this end, the copy number of Rhgl and Rhg4 was determined in the parental lines, one susceptible line, one resistant line, and one moderately susceptible line using the Comparative Genomic Hybridization (CGH) array. Williams 82, which contains a single copy at Rhgl and Rhg4.jwas used as a reference and calibrator. The CGH array showed approximately three copies of Rhgl and one copy of Rhg4 in the three RILs and their parents compared to the single copy detected in Williams 82 (Figures IB and 1C). The absence of any variation in copy number of Rhgl and Rhg4 in the RILs indicates that differences in resistance to Hg Type 1.2.5.7 among the RILs is independent of copy number of Rhgl and Rhg4. Third, we examined if differential expression of GmSNAP18 and GmSHMT08 among the resistant and susceptible RILs contributes to the phenotypic variability. Therefore, we quantified the expression levels of GmSNAP18 and GmSHMT08 in various resistant and susceptible RILs at 3, 5, 7, and 9 days post infection (dpi) by Hg Type 1.2.5.7. No significant differences in the expression of GmSNAP18 and GmSHMT08 genes were detected between the resistant and susceptible RILs at any time point. Collectively, these analyses indicate that resistance to Hg Type 1.2.5.7 in our RILs is independent of SCN resistance genes Rhgl and Rhg4 and controlled by as yet unidentified gene(s).

[0420] EXAMPLE 2— SNP ARRAY GENOTYPING AND GENETIC MAP CONSTRUCTION

[0421] To identify the gene(s) controlling soybean resistance to SCN Hg Type 1.2.5.7 we used map-based cloning approach to construct a genetic map and linkage groups (LGs), identify QTL associated with resistance, and finally identify gene candidates for functional validation. We first generated ingle nucleotide polymorphism (SNP) data for the 114 RILs and the two parents. Total DNA was extracted from these RILs and the two parents and used for SNP analysis using Illumina (San Diego, CA) SoySNP50K iSelect BeadChip, which contains a total of 52,041 SNPs equally distributed across the 20 soybean chromosomes (Song et al., 2015). Raw SNP data were filtered and the positions were mapped to the most recent version of soybean genome (Wm82.a4.vl). High-quality nonredundant SNP markers were used construct the genetic map using QTL IciMapping (Meng etal., 2015), and subsequently the linkage map. More than 400 polymorphic markers with distinct segregation patterns were identified and used to construct the linkage map. A total of 53 LGs were established by mapping the markers to their physical locations on the 20 soybean chromosomes. We performed inclusive composite interval mapping (ICIM) analysis using cyst number and cyst index data with a=0.05 as a significance cut off. The permutation test determined a logarithm of the odds (LOD) value of 3.0 as a significant threshold for genome-wide analysis.

[0422] EXAMPLE 3— IDENTIFICATION OF QTL ASSOCIATED WITH RESISTANCE TO SCN HG TYPE 1.2.5.7

[0423] Two QTL associated with resistance to SCN Hg Type 1.2.5.7 were identified. The first QTL (SCN-R2-1) was mapped to Chr. 14 with a LOD score of 4.8 and showed the highest phenotypic variance explained (PVE) value of 19% and an additive effect of -5.8% on the FI value. The second QTL (SCN-R2-2) was mapped to Chr. 16 with a LOD score of 3.4. This QTL explained 13.3% of the total phenotypic variance with an additive effect of -4.84. Both QTL showed negative additive effects, suggesting that alleles inherited from the parental line TN09-29 are responsible for resistance to SCN Hg Type 1.2.5.7. No epistatic association was identified between the identified QTL. EXAMPLE 4— WHOLE-GENOME SEQUENCING OF RILS, FINE-MAPPING OF QTL, AND IDENTIFYING RESISTANCE GENE CANDIDATES

[0424] To narrow the intervals found in each QTL and identify SCN resistance gene candidates, we sequenced the whole genome of 4 susceptible RILs, 4 resistant RILs, and the two parents with 20* coverage. Genomic DNA was extracted and Illumina paired-end libraries were constructed and sequenced using Illumina HiSeq XTen platform. After quality filter and trimming, more than 130 M Illumina paired-end reads (150 bp) per genotype were aligned to the soybean reference genome using Burrows- Wheel er Aligner (BWA). Variants were called and quality filtered were determined using the Genome Analysis Toolkit (GATK v4). The variants from whole genome sequencing data in each of the 2 QTL interval were used to determine the resistant haplotypes that exist in all the 4 resistant RILs and absent in Williams 82 reference genome and all the 4 susceptible RILs. Haplotype analysis using the Variant Effect Predictor (VEP) online tool (see Worldwide Website: plants.ensembl.org / Glycine_max / Tools / VEP) resulted in the identification of 56 and 3 non- synonymous mutations in QTL SCN-R2-1 and QTL SCN-R2-2, respectively. We next examined the presence of the resistant variants of the 56 non-synonymous mutations in 94 soybean accessions, which are susceptible to Hg Type 1.2.5.7 (Patil etal., 2019). The resistant variants of 51 non-synonymous mutations were detected in at least 42 susceptible soybean accessions, and therefor excluded from further analysis. The remaining 5 non-synonymous mutations are unique to the resistant RILs and overlap with 3 protein-coding genes (Glyma.14G050600, Glyma.14G050700, and Glyma.l4G056000.2) (Table 2). The 3 missense variants detected in QTL SCN-R2-1 were located in one gene (Gylma.l6G137000) encoding a disease resistance protein (TIR-NBS-LRR class) family (Table 2).

[0425] Table 2: Amino acid substitutions in susceptible and resistant RILs detected in the four candidate resistance genes. EXAMPLE 5— GLYMA.14G050600 ENCODING A PROTEIN DISULFIDE ISOMERASE- LIKE (PDIL) IS A NEW SOYBEAN GENE CONFERRING HIGH LEVEL OF RESISTANCE TO SCN HG TYPE 1.2.5.7.

[0426] We quantified the transcript abundance of the three candidate resistance genes in root tissues of a resistant (RIL-137) and a susceptible (RIL-72) lines at 5 dpi with SCN Hg Type 1.2.5.7 using quantitative RT-PCR (qPCR). We also quantified the expression levels of variant 1 and 2 of Glyma.l4G056000.2. Data from three biological replicates revealed only the expression level of Glyma. 14G050600 was significantly increased in the resistant RIL-137 as compared with the corresponding non-infected root samples (Figure 2), pointing to Glyma. 14G050600 as the likely SCN resistance gene.

[0427] We further examined whether overexpression of the resistant variants of the candidate resistance gene Glyma.14G050600 would enhance soybean resistance to SCN Hg Type 1.2.5.7. We generated transgenic hairy root plants overexpressing the coding sequence of the resistant and susceptible variants of Glyma.14G050600 under the control of a soybean ubiquitin promoter in the susceptible line RIL-72 using transgenic hairy root system. Transgenic soybean roots were identified and selected using the green fluorescent protein (GFP). Composite hairy root plants expressing the empty vector containing the GFP marker gene were used as control. Composite hairy root plants overexpressing the resistant variant of Glyma.14G050700 was also used as an additional control. Root structure and morphology of the overexpression lines were undistinguishable from that of the control plants. After selection, each plant was inoculated with about 2000 eggs of SCN Hg Type 1.2.5.7, with each line being replicated about 20 times. SCN resistance were determined 5 weeks after inoculation by counting the number of cysts per plant. Cyst numbers were used to calculate the cyst index, which was calculated by dividing the average number of cysts on the transgenic hairy root lines by the average number of cysts determined on the control plants expressing the empty vector multiplied by 100. Importantly, composite plants overexpressing the resistant variant of Glyma.14G050600 significantly enhanced plant resistance with 75% reduction in female index. Overexpressing the susceptible variant of Glyma.14G050600 also enhanced plant resistance but to a much lesser extent showing only 32% reduction in female index (Figure 3). Overexpressing the resistant variant of Glyma.14G050700 showed no significant effect on soybean resistance to SCN (Figure 3). Collectively, these functional data confirm the function of Glyma.14G050600 as a resistance gene conferring high level of resistance to SCN Hg Type 1.2.5.7. This newly identified SCN resistance gene (Glyma.l4G050600) encodes a protein disulfide isomerase-like (PDIL). This protein contains a signal peptide (aa 1-28), several thioredoxin domains, and a classical C-terminal tetrapeptide EDEL endoplasmic reticulum (ER)-retention signal. The resistant haplotype contains nonsynonymous substitutions resulting in Ser to Leu and Vai to Leu changes at position 26 and 404, respectively (Figure 3). Protein disulfide isomerases are highly conserved in plant and animal systems and catalyze disulfide bond formation and isomerization through the thioredoxin domains (Ellgaard and Ruddock 2005; Houston et al., 2005). Protein disulfide isomerases function in protein quality control in the ER and contribute to protein folding, stabilization, and transport (Maattanen et al., 2010). A role of protein disulfide isomerases in plant-pathogen interactions was reported (Yang etal., 2014; Faheem et al., 2016).

[0428] EXAMPLE 6— SILENCING GLYMA.03G054100, GLYMA.10G285300, AND GLYMA.04G225500 SIGNIFICANTLY ENHANCED SOYBEAN RESISTANCE TO SCN HG TYPE 1.2.5.7

[0429] We generated RNA-seq libraries from syncytium-enriched roots tissues collected from the susceptible (RIL-72) and resistant (RIL-137) lines at 5-day post infection (dpi) with SCN Hg Type 1.2.5.7. Using a false discovery rate < 5%, we identified 874 and 2571 differentially expressed genes (DEGs) in the susceptible and resistant lines, respectively, in comparison with the corresponding non-infected control roots (Figure 4). Cross comparison of upregulated and downregulated genes in both lines revealed that 8 genes were oppositely regulated in both lines (Figure 4). Five (Glyma.03G054100, SEQ ID NO: 17, Glyma.04G225500, SEQ ID NO: 21, Glyma.l0G285300, SEQ ID NO: 19, Glyma.l2G216900 (SEQ ID NO: 29), and Glyma.U032805 (SEQ ID NO: 31)) of these 8 genes were upregulated in the susceptible line (RIL-72) and downregulated in the resistant line (RIL-137), making them excellent candidates for enhancing soybean resistance to SCN Hg Type 1.2.5.7 using non-GMO CRISPR genome editing approach. The remaining 3 genes (Glyma.03G253600 (SEQ ID NO: 23), Glyma.04Gl 85400 (SEQ ID NO: 25), and Glyma.l8G244600 (SEQ ID NO: 27)) were downregulated in the susceptible line (RIL-72) and upregulated in the resistant line (RIL-137), making them good candidates for enhancing soybean resistance to SCN Hg Type 1.2.5.7 using transgenic overexpression approach.

[0430] We used RNA interference (RNAi) approach to silence the expression of 4 genes (Glyma.03G054100 (SEQ ID NO: 17), Glyma.04G225500 (SEQ ID NO: 21), Glyma.10G285300 (SEQ ID NO: 19), and Glyma. l2G216900 (SEQ ID NO: 29)). About 300 bp DNA fragments specific to each of these 4 genes were synthesized and cloned in the pTRV2 vector. The 5 pTRV2 vectors were introduced into Agrobacterium tumefaciens strain GV2260 and used for gene silencing experiments. Seeds of the susceptible soybean line (RIL-72) were planted in pots filled with steam-sterilized sand mixed with topsoil. Five-day-old soybean seedlings were infiltrated with agrobacterium solutions. Each of the 5 constructed silencing vectors was applied to at least 20 replicated plants. Soybean seedlings inoculated with Agrobacterium containing the empty pTRV2 vector were used as control. Gene silencing of each of these 5 genes reached its peak about 12 days after agrobacterium inoculation. Twelve days after agrobacterium inoculation, each soybean seedling was inoculated with about 2000 eggs of SCN Hg Type 1.2.5.7. Five weeks after inoculation, SCN cysts were extracted from each plant separately and used to calculate cyst index, and subsequently plant resistance as described above. Notably, silencing Glyma.03G054100 (SEQ ID NO: 17), Glyma.10G285300 (SEQ ID NO: 19), and Glyma.04G225500 (SEQ ID NO: 21) significantly enhanced plant resistance with 79%, 65%, and 62 reduction in cyst index (Figure 5). Silencing of Glyma.12G216900 (SEQ ID NO: 29), however, showed non-significant reduction in cyst index of 14% (Figure 5). These data support the utilization of these 3 genes (Glyma.03 G054100 (SEQ ID NO: 17), Glyma.10G285300 (SEQ ID NO: 19), and Glyma.04G225500 (SEQ ID NO: 21)) for non-GMO CRISPR genome editing to improve soybean resistance to SCN Hg Type 1.2.5.7.

[0431] It should be understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and the scope of the appended claims. In addition, any elements or limitations of any invention or embodiment thereof disclosed herein can be combined with any and / or all other elements or limitations (individually or in any combination) or any other invention or embodiment thereof disclosed herein, and all such combinations are contemplated within the scope of the invention without limitation thereto. REFERENCES

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Claims

CLAIMSI claim:

1. A plant cell comprising at least one inactivated gene or an underexpressed gene, wherein the at least one inactivated gene or the underexpressed gene comprises a sequence selected from SEQ ID NO: 1, 2, 3, 4, 17, 19, 21, 29, or 31 and combinations thereof.

2. The plant cell of claim 1, wherein the plant cell comprises: i) one or more mutations in the endogenous promoter of the inactivated gene or the underexpressed gene that inhibits or abolishes the expression of the inactivated gene or the underexpressed gene; ii) an inhibitory oligonucleotide that inhibits or abolishes the expression of the inactivated gene or the underexpressed gene; iii) a deletion of the inactivated gene or the underexpressed gene; or iv) the inactivated or underexpressed gene integrated into the germplasm of the plant cell or the plant.

3. The plant cell of any of claims 1 to 2, wherein the plant cell is in a plant part.

4. The plant cell of claim 3, wherein the plant part is a seed, endosperm, ovule or pollen.

5. The plant cell of any of claims 1 to 4, wherein the plant is a soybean plant or other cyst nematode-host plants.

6. A plant cell comprising at least one gene, wherein the at least one gene is selected from SEQ ID NO: 5, 6, 7, 8, 23, 25, or 27 and combinations thereof.

7. The plant cell of claim 6, wherein the plant cell comprises: i) in the endogenous promoter of the gene one or more mutations that increase the expression of the gene; ii) a heterologous nucleic acid comprising the gene; iii) the gene integrated into the germplasm of the plant cell or the plant; or iv) the gene operably linked to a soybean endogenous promoter and / or an exogenous promoter.

8. The plant cell of any of claims 6 to 7, wherein the plant cell is in a plant part.

9. The plant cell of claim 8, wherein the plant part is a seed, endosperm, ovule or pollen.

10. The plant cell of any of claims 6 to 9, wherein the plant is a soybean plant or other cyst nematode-host plants.

11. A nucleic acid construct comprising an exogenous promoter operably linked to SEQ ID NO: 5, 6, 7, 8, 23, 25, or 27.

12. The nucleic acid construct according to claim 11, wherein the exogenous promoter is the soybean ubiquitin promoter.

13. The nucleic acid construct according to claim 11, wherein the exogenous promoter is a constitutive promoter, an inducible promoter, or a tissue-specific promoter.