Agricultural pesticide composition and use thereof

Abstract

The present disclosure describes an agricultural pesticide composition and an agricultural pesticide method using same, wherein the agricultural pesticide composition exhibits a strong pesticidal effect against pests while having low toxicity to humans and low risk of environmental pollution when a mixture of rotenone as an insecticidal ingredient and an pesticide efficacy enhancer is sprayed on crops, and may also increase cell vitality of plants, mitigate foul odors, and promote emotional stability of the human body.

Description

Agricultural pesticide composition and use thereof

[0001] The present disclosure relates to an agricultural pesticide composition and its use. More specifically, the present disclosure relates to an agricultural pesticide composition and a method for killing agricultural pests using the same, which combines rotenone as an insecticidal ingredient and an insecticide potency enhancer to exhibit a strong insecticidal effect against pests while having low toxicity to humans and low risk of environmental pollution when sprayed on crops, and which can also increase plant cell vitality, solve the problem of offensive odor, and provide emotional stability to the human body.

[0002] Pests are insects that directly or indirectly harm human life. Recently, damage caused by pests has been increasing due to environmental and ecological changes such as global warming and ecosystem disruption.

[0003] In this regard, the use of pesticides is essential to control pests that interfere with crop cultivation and growth and to increase crop productivity. In particular, it is customary to spray pesticides at least several times during the crop's growth cycle. With the rural population steadily declining and the agricultural workforce aging, there is a growing need to conserve labor required for crop cultivation and management. Interest in pesticides, particularly to reduce the labor required for pest control, is steadily increasing.

[0004] Currently, synthetic chemical pesticides are primarily used in agricultural production. However, their excessive use is a major cause of soil and water pollution. Furthermore, the use of synthetic chemical pesticides poses a threat to the health of workers involved in pesticide application and crop cultivation. Furthermore, the high concentration of chemical compounds in agricultural products poses a threat to consumer health.

[0005] As an alternative, interest is growing in low-toxicity (non-toxic) natural pesticides manufactured from various microorganisms and natural materials. Compared to synthetic pesticides, natural pesticides have less toxicity on humans and livestock, and are rapidly decomposed in the natural environment, making them effective in preventing environmental pollution.

[0006] Nevertheless, the aforementioned natural pesticides based on these ingredients have low acceptance in agricultural settings due to low confidence in their insecticidal efficacy. Specifically, while they are primarily based on microbial or plant extracts, they are not commercially available in diverse formulations. Therefore, some research has been conducted to develop various additives, supplements, and efficacy-enhancing ingredients and utilize them to enhance the performance of eco-friendly pesticides (e.g., Korean Patent No. 1882678).

[0007] However, when using ingredients that enhance the effectiveness of previously known natural pesticides, the effect is minimal and problems such as the generation of unpleasant odors from the product itself arise, even though it provides the effect of reducing toxicity to the human body and the risk of environmental pollution.

[0008] In this way, when combining natural pesticide ingredients with their effect-enhancing ingredients, a method is required to solve the problem of odor while enhancing the insecticidal effect, in addition to the advantages of using natural ingredients.

[0009] In one specific example of the present disclosure, when a natural pesticide is sprayed on crops by combining it with a novel effect-enhancing ingredient, it is intended to provide an agricultural pesticide composition that exhibits a strong pesticidal effect against pests while having low toxicity to humans and low risk of environmental pollution, and can also increase cell vitality of plants, solve the problem of bad odor, and provide emotional stability to the human body.

[0010] Another specific example of the present disclosure is to provide a method for killing agricultural pests using an agricultural pesticide composition.

[0011] According to the first page of this disclosure,

[0012] An agricultural pesticide composition comprising rotenone as an insecticide: a composition for enhancing the effectiveness of an insecticide in a ratio of 0.1 to 5:95 to 99.9 by weight,

[0013] At this time, the composition for enhancing the effectiveness of the pesticide is

[0014] (i) 40 to 90 wt% of a perilla extract;

[0015] (ii) 1 to 20 wt% of soybean oil;

[0016] (iii) 1 to 30 wt% of dill seed oil; and

[0017] (iv) 1 to 20 wt% of natural lecithin emulsifier;

[0018] An insecticide composition comprising:

[0019] According to an exemplary embodiment, the above-mentioned extract of the plant may be obtained using alcohol as an extraction solvent.

[0020] According to an exemplary embodiment, the alcohol may be alcohol at a concentration of at least 30% (v / v).

[0021] According to an exemplary embodiment, the alcohol may be an aliphatic alcohol having 1 to 5 carbon atoms.

[0022] According to an exemplary embodiment, the alcohol may be at least one selected from the group consisting of ethanol, isopropanol, 1-propanol, isobutanol, n-butanol, butan-2-ol and t-butanol.

[0023] According to an exemplary embodiment, the agricultural pesticide composition may be in at least one formulation selected from the group consisting of granules, powders, liquids, aerosols, sprays, extracts, pastes, fluid extracts, emulsions, suspensions, capsules, liquid wettable powders, granule wettable powders, wettable powders, powders, granules, oils, gel-type formulations, smokers, foggers, and fumigants.

[0024] According to the second aspect of the present invention,

[0025] A step of spraying the aforementioned agricultural pesticide composition;

[0026] A method for killing agricultural pests including:

[0027] According to an exemplary embodiment, the spraying step may be performed in a heated mist manner.

[0028] According to an exemplary embodiment, the agricultural pest may be at least one selected from the group consisting of aphids, whiteflies, moths, shield beetles, weevils, leafhoppers and scale insects.

[0029] According to an exemplary embodiment, the agricultural pest may be an adult or a larva.

[0030] According to one specific example, an agricultural pesticide composition comprises rotenone as an insecticidal ingredient, in combination with a pesticide efficacy enhancing composition containing a plurality of natural extracts and oils, and a natural emulsifier, thereby maximizing the insecticidal effect of rotenone. In particular, by composing the pesticide composition with natural materials, it overcomes the disadvantages of chemically synthesized pesticides, which can cause toxicity to the human body and environmental pollution, and can also reduce pest resistance caused by the use of chemical agents. Furthermore, the oil contained in the efficacy enhancing composition exhibits antibacterial properties, thereby having a control effect against powdery mildew, which is a problem in crop facility cultivation, and has a beneficial effect on the emotional stability of the human body. The natural lecithin emulsifier can provide the additional benefit of enhancing the vitality of plant cells. Therefore, it is expected that the composition will be usable in both urban and rural areas in the future.

[0031] Figure 1 is a photograph schematically showing the sequence of manufacturing a composition for enhancing the effectiveness of an insecticide;

[0032] Figure 2 shows the results of a test for the presence or absence of a toxic substance for a composition for enhancing the effectiveness of an insecticide;

[0033] Figure 3 is a photograph schematically showing a process for preparing an insecticide composition by combining rotenone and an efficacy enhancing composition;

[0034] Figure 4 is a graph showing the results of evaluating the insecticidal effect against tobacco whiteflies (adults), cotton aphids, American silverbacks (nymphs), rice water weevils, and peach aphids, respectively, using a 500-fold dilution of an insecticidal composition prepared by combining rotenone and an efficacy enhancing composition;

[0035] Figure 5 is a graph showing the results of evaluating the insecticidal effect against American white moths (1-3 instars), American white moths (4-5 instars), and azalea beetles, respectively, using a 500-fold dilution of an insecticidal composition prepared by combining rotenone and an efficacy enhancing composition;

[0036] Figure 6 is a graph showing the results of evaluating the insecticidal effect by the number of treatments when a 500-fold diluted insecticide composition prepared by combining rotenone and an efficacy enhancing composition was treated at 3-day intervals against mealybugs;

[0037] Figure 7 is a graph showing the results of evaluating the insecticidal effect 3 days after treatment of 500-fold diluted solutions of each insecticidal composition prepared by combining two commercially available materials and rotenone and an efficacy enhancing composition against cotton aphids; and

[0038] Figure 8 is a graph showing the results of evaluating the insecticidal effect 3 days after treatment of 500-fold diluted solutions of each insecticidal composition prepared by combining two commercially available materials and rotenone and an efficacy enhancing composition against tobacco whitefly.

[0039] The present invention can be fully achieved by the following description. It should be understood that the following description describes preferred embodiments of the present invention and is not necessarily limited thereto. Furthermore, the attached drawings are provided to aid understanding and are not intended to limit the present invention.

[0040] In this specification, the terms used may be defined as follows.

[0041] "Crops" or "crops" can be understood as a concept that includes both food crops and industrial crops, such as grains such as rice, barley, wheat, rye, oats, corn, and sorghum; legumes such as soybeans, red beans, beans, peas, and peanuts; fruit trees and fruits such as apples, tangerines, oranges, lemons, limes, grapes, quinces, pears, blueberries, cranberries, blackberries, raspberries, strawberries, peaches, plums, nectarines, plums, yellow peaches, apricots, plums, walnuts, almonds, pistachios, cashews, bananas, persimmons, olives, and palms; vegetables such as cabbage, tomatoes, spinach, broccoli, lettuce, onions, green peppers, eggplant, turnip cabbage, Chinese cabbage, mustard greens, asparagus, carrots, pursley, and celery; root vegetables such as carrots, burdock, potatoes, sweet potatoes, yams, taro, radishes, lotus roots, and turnips; Crops for processing, such as cotton, hemp, paper mulberry, rapeseed, oil palm, buckwheat, sugarcane, sugar beet, olive, rubber, coffee, tobacco, mulberry, and tea; fruits, such as pumpkin, cucumber, watermelon, and melon; pastures, such as sorghum and clover; grasses, such as grass and rye; crops used as spices, such as lavender, rosemary, thyme, pepper, chili pepper, horseradish, ginger, garlic, mint, and basil; flowers, such as roses, carnations, chrysanthemums, baby's breath, sunflowers, marigolds, tulips, lilies, lavender, and begonias; Examples of trees include ash, birch, American dogwood, eucalyptus, ginkgo, lilac, maple, oak, poplar, birch, maple, sycamore, zelkova, locust, fir, pine, Japanese pine, juniper, pine, spruce, yew, elm, horse chestnut, coral, fir, Japanese cypress, Japanese cypress, and Japanese oak.

[0042] "Agricultural pest" can be narrowly defined as an agricultural or horticultural pest that causes harm by sucking or gnawing on crops, but in some cases, it can be understood as a concept that includes forest pests and sanitary pests. In addition, agricultural pests can be adults or larvae. Examples of such agricultural pests include Lepidoptera, Hemiptera, Herbivores, Diptera, Hymenoptera, Ornithoptera, Thrips, Acari, Termites, Cockroaches, and Fleas. More specifically, agricultural pests include aphids, whiteflies, moths, shield beetles, weevils, leafhoppers, and scale insects. For example, aphids can be at least one selected from the following: cotton aphid, green peach aphid, rhododendron aphid, Japanese maple aphid, Japanese sesame aphid, perilla aphid, and hairy aphid. The whitefly may be at least one selected from the greenhouse whitefly, tobacco whitefly, etc. The moth may be at least one selected from the peach borer, peach borer, corn borer, cutworm, peach heart borer, apple heart borer, black-spotted day borer, gypsy moth, tent moth, pine moth, American white moth, etc. The shield insect may be at least one selected from the plane tree shield beetle, azalea shield beetle, pear shield beetle, ash shield beetle, oak shield beetle, macula chinensis shield beetle, etc. Meanwhile, the weevil may be at least one selected from the chestnut weevil, acorn chestnut weevil, rice water weevil, apple goblin weevil, etc. In addition, the cicadas may be at least one selected from the group consisting of a bride-winged cicada, a fan-winged cicada, a Japanese-winged cicada, a southern-winged cicada, a black-winged cicada, a black-tipped cicada, a mottled cicada, a large mottled cicada, a black-spotted cicada, a large Korean pointed cicada, a wavy pointed cicada, a white-lined yellow cicada, a white-spotted yellow cicada, a tip-winged cicada, a two-spotted tip-winged cicada, a black bear cicada, a brown-winged cicada, and an American fairy bug.In addition, the scale insects may be at least one selected from, for example, the maple scale insect, the persimmon scale insect, the horned mealybug insect, the turtle scale insect, the mealybug insect, the brown scale insect, the mulberry scale insect, the maple scale insect, the magnolia scale insect, and the tangerine yellow scale insect.

[0043] In this specification, when a specific numerical range is described, it may be considered that any value within the range, as well as any sub-combination of any two values ​​within the range as the lower and upper limits, are described. For example, "1 to 5" may be understood to describe the values ​​1, 2, 3, 4, and 5, as well as any sub-combination.

[0044] The expressions "on" and "above" can be understood as referring to relative positional concepts. Accordingly, not only may other components or layers be directly present on the mentioned layer, but at least one other layer (an intermediate layer or intervening layer) may also be present between them, or additional components may be present or interposed. Similarly, the expressions "below," "underneath," and "beneath," as well as the expression "between," can also be understood as relative positional concepts. Furthermore, the expression "sequentially" can also be understood as a relative positional concept.

[0045] When we say that a component or step "comprises", we mean that it may further comprise other components and / or steps, unless otherwise stated.

[0046] An agricultural pesticide composition according to one specific example of the present disclosure can maximize the control effect and minimize the environmental toxicity of environmental destruction by combining rotenone as an insecticide or insecticidal ingredient and a composition for enhancing the effectiveness of an insecticide derived from a natural product.

[0047]

[0048] Composition for enhancing the effectiveness of pesticides

[0049] According to one specific example, a component derived from a natural product may be used as a main component of a composition for enhancing the effectiveness of an insecticide, and optionally or additionally, a biocompatible additive component may be further contained in the composition for enhancing the effectiveness.

[0050]

[0051] - Extract of perilla

[0052] In one specific example, a perilla extract may be used as a potency-enhancing ingredient. Perilla (Rabdosia japonica) is a perennial herb distributed throughout East Asia and is also known as "long-life herb." Perilla has been primarily used as a medicine, and research has focused on its efficacy in treating gastrointestinal disorders, tumors, and inflammatory conditions. It is known to contain approximately 24 main components.

[0053] In this specific example, a perilla extract is used, and although the present disclosure is not bound by a specific theory, it is believed that the perilla extract contains the component estragole, and thus performs the function of weakening insect immunity by its own insecticidal power in the process of enhancing the effectiveness of the insecticide.

[0054] Meanwhile, the extraction of the perilla leaf can be performed using methods known in the art, such as a moisture absorption method, a supercritical fluid extraction method, a water extraction method, an alcohol extraction method, a shaking extraction method under high temperature / high pressure conditions, a low temperature shaking extraction method, etc., but specifically, an alcohol extraction method can be used. The alcohol extraction method can be advantageous in that it can obtain the active ingredient simply and quickly. At this time, the alcohol as the extraction solvent can be, for example, an aliphatic alcohol having 2 to 5 carbon atoms. Such alcohol can be, for example, at least one selected from ethanol, isopropanol, 1-propanol, isobutanol, n-butanol, butan-2-ol, t-butanol, etc. According to a specific embodiment, an alcohol equivalent to ethanol can be used as the alcohol, which can be preferable in terms of providing a natural ingredient. According to an exemplary embodiment, the concentration of the alcohol may be, for example, at least about 30% (v / v), specifically at least about 40% (v / v), more specifically at least about 50% (v / v). According to a specific embodiment, the concentration of the alcohol may be adjusted to, for example, at least about 60% (v / v), specifically at least about 65 to 95% (v / v), more specifically in the range of about 70 to 90%. According to a specific embodiment, ethanol (spirit) may be used as the extraction solvent at a concentration of at least about 70% (v / v).

[0055] According to the present specific example, the content of the extract of the perilla frutescens in the composition for enhancing the efficacy of an insecticide can be set in consideration of the content of estragole, and can be adjusted in the range of, for example, about 40 to 90 wt%, specifically about 43 to 85 wt%, and more specifically about 45 to 85 wt%, based on the total weight of the composition for enhancing the efficacy. In a specific specific example, the content of the extract of the perilla frutescens can be adjusted in the range of, for example, about 50 to 80 wt%, specifically about 52 to 75 wt%, more specifically about 55 to 70 wt%, and most specifically about 57 to 60 wt%. However, the above-mentioned content range can be understood as an exemplary one, and can be changed in consideration of the content of other components in the composition, the type of crop to be insecticided, etc.

[0056] According to an exemplary specific example, the process for obtaining a perilla extract by an alcohol extraction method can be described as follows.

[0057] Dried perilla leaves are added to an alcohol solvent to form a perilla leaves-alcohol mixture. Typically, the alcohol extraction solvent may be used in an amount determined in the range of, for example, about 500 to 3000 parts by weight, specifically about 800 to 2500 parts by weight, and more specifically about 1000 to 2000 parts by weight, based on 100 parts by weight of perilla leaves, but is not limited thereto. At this time, perilla leaves may be introduced into the alcohol solvent in the form of a pulverized product, and pre-dried perilla leaves may be pulverized using a means known in the art, for example, a hammer mill, a jaw crusher, a gyratory crusher, etc. In addition or alternatively, a step of pulverizing perilla leaves using an ultrasonic pulverizer after introducing them into the alcohol solvent may be further performed.

[0058] Next, the perilla extract can be obtained by concentrating the perilla-alcohol mixture and then freeze-drying it. At this time, filtering can be performed, and concentration can be performed using a rotary evaporator. Since this post-processing step, i.e., the separation and purification step, is well known in the art, a separate description is omitted.

[0059]

[0060] - Soybean oil

[0061] According to one specific example, the composition for enhancing efficacy contains soybean oil as a natural ingredient.

[0062] "Soybean oil" may refer to a semi-drying oil extracted from soybeans, and is also called soybean oil. Soybean oil is mainly composed of oleic acid, linoleic acid, and oleic acid, and is widely used for edible and industrial purposes. The amount of soybean oil contained in soybeans is approximately 20%, and can be obtained through processing such as solvent extraction using a petroleum solvent (e.g., hexane, etc.), heating, and distillation. For example, soybean oil may contain about 14 to 16 wt% saturated fatty acids, about 16 to 32 wt% oleic acid, about 47 to 61 wt% linoleic acid, and about 6 to 8 wt% linolenic acid, but this may be understood as an example.

[0063] In an exemplary embodiment, soybean oil is believed to perform a surfactant function in the process of enhancing the efficacy of the pesticide. In this regard, the content of soybean oil in the efficacy-enhancing composition can be set in consideration of the amount of the natural lecithin emulsifier described below, and can be adjusted, for example, in the range of about 1 to 20 wt%, specifically about 3 to 15 wt%, and more specifically about 5 to 10 wt%, based on the total weight of the efficacy-enhancing composition. The above-described content range can be understood as an exemplary one.

[0064]

[0065] - Dill seed oil

[0066] According to one specific example, the potency-enhancing composition contains dill seed oil as a natural ingredient. Dill is a type of herb (specifically, Anethum graveolens) and is primarily distributed in Europe and Southwest Asia, including India, Pakistan, Egypt, the United Kingdom, Hungary, and Germany. Dill seed oil can be obtained by processing the seeds through steam distillation, solvent extraction, or supercritical extraction.

[0067] Specifically, steam distillation involves placing dill seeds in a stainless steel vessel, passing high-pressure steam through them, and extracting the resulting dill seed oil as a distillate using a fractional distillation column. Solvent extraction can be performed using both low-temperature and high-temperature solvent extraction, and in some cases, may be performed as an additional step after the aforementioned steam distillation. Meanwhile, supercritical extraction utilizes carbon dioxide as an extraction medium and extracts oil from dill seeds under supercritical conditions.

[0068] Dill seed oil obtained through the above-described process may contain d-carvone, d-limonene, d-phellandrene, α-pinene, diterpene, d-dihydrocarvone, etc.

[0069] In this specific example, dill seed oil can perform the function of weakening insect immunity due to its own insecticidal property during the process of enhancing the efficacy of the insecticide. In addition, the content of dill seed oil in the composition for enhancing efficacy can be set in consideration of the content of the insecticidal raw material, and can be adjusted in the range of, for example, about 1 to 30 wt%, specifically about 2 to 25 wt%, more specifically about 5 to 20 wt%, and particularly specifically about 7 to 10 wt%, based on the total weight of the composition for enhancing efficacy. The above-mentioned content range can be understood as an exemplary one.

[0070]

[0071] - Natural lecithin emulsifier

[0072] According to one specific example, the composition for enhancing efficacy contains a natural emulsifier. Typically, an "emulsifier" is a substance containing both hydrophilic and hydrophobic groups within its molecule. It can refer to a state in which two liquid phases with low miscibility are dispersed in one another by applying mechanical energy to form an emulsion. Thus, an emulsifier can refer to a component that stabilizes a dispersion or a dispersion liquid, thereby contributing to the long-term storage of the composition.

[0073] In this specific example, a natural lecithin emulsifier is used as the emulsifier. Lecithin is a mixture of polar lipids, and refers to a group of yellowish-brown fatty substances derived from plant and animal tissues, consisting of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides, and phospholipids, and can be extracted from soybeans, egg yolks, etc. For example, lecithin can be extracted through a degumming process of vegetable oil. At this time, the main components of lecithin can be phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol. For example, the HLB (hydrophile-lipophile balance) of lecithin can be, for example, in the range of about 3 to 9, specifically about 3.5 to 8, and more specifically about 4 to 7.

[0074] In the present specific example, the content of the natural lecithin emulsifier in the composition for enhancing efficacy may be adjusted in a range of, for example, about 1 to 20 wt%, specifically about 5 to 17 wt%, more specifically about 8 to 15 wt%, and particularly specifically about 10 to 13 wt%, based on the total weight of the composition for enhancing efficacy, and the above-mentioned content range may be understood as an exemplary range.

[0075]

[0076] - Added ingredients

[0077] According to the present specific example, the composition for enhancing efficacy may further contain various additional ingredients, for example, an agronomically acceptable carrier, etc.

[0078] According to an exemplary embodiment, the composition for enhancing the efficacy of a pesticide may be used alone or in the form of a mixture with solid and / or liquid carriers, vehicles and / or other compatible active agents known in the art, such as plant protection agents such as other insecticides, arachnides, miticides, nematicides, fungicides, bactericides, rodenticides, herbicides, fertilizers, growth regulators, etc., or in a ready-to-use form, such as a dosage form prepared therefrom for a specific application, as required, such as a solution, emulsion, suspension, powder, paste and granules. In the case of an agronomically acceptable carrier, it may be an inert carrier of inorganic or organic, or synthetic or natural origin, and may be a material with which the composition is mixed or formulated to facilitate the plant, seed, soil or other target for treatment, or the storage, transport and / or handling thereof. In particular, for insecticides, herbicides or antibacterial agents, any inert carrier that can be used as a carrier can be used.

[0079] According to exemplary embodiments, in the case of the solid carrier, there are natural minerals such as quartz, clay, kaolin, pyrophyllite, sericite, talc, bentonite, acid clay, attapulgite, zeolite, diatomaceous earth, etc.; inorganic salts such as calcium carbonate, ammonium sulfate, sodium sulfate, potassium chloride, etc.; organic solid carriers such as synthetic silicic acid, synthetic silicate, starch, cellulose, plant powder (e.g., sawdust, palm husk, corn cob, tobacco stem, etc.); plastic carriers such as polyethylene, polypropylene, polyvinylidene chloride, etc.; urea; inorganic hollow bodies; plastic hollow bodies; fumed silica, etc. These may be used alone or in combination of two or more.

[0080] In addition, the composition for enhancing efficacy may be formulated by mixing with an inert diluent or swelling agent of a type applicable to optionally insecticidal preparations, such as a gas, a solution, an emulsion, a suspension, an emulsifiable concentrate, a spray powder, a paste, a soluble powder, a dust collector, a granule, a foaming agent, a paste, a tablet, an aerosol, a natural and synthetic substance mixed with an active compound, a microcapsule, a coating composition for use on seeds, and a preparation for use with burning equipment such as a flammable cartridge, an effervescent can, and an effervescent coil, as well as a ULV cold fog and warm fog preparation. It is preferable to appropriately adjust the amount of the additional components such as the carrier described above, the amount of use, etc., depending on the formulation, spraying location, and spraying method of the composition for enhancing efficacy.

[0081]

[0082] pesticide composition

[0083] According to one specific example of the present disclosure, rotenone, a natural insecticide, is used as a component that provides the original insecticidal effect in combination with the aforementioned efficacy enhancing composition.

[0084] Rotenone is obtained from the roots of the legumes Derris elliptica, Lonchocarpus, and Tephrosia, and is a heteropentacyclic compound, i.e., an isoflavonoid, represented by the following chemical formula 1.

[0085]

[0086] [Chemical Formula 1]

[0087]

[0088]

[0089] Rotenone is a naturally occurring insecticidal ingredient known to inhibit ATP synthesis in insect electron transport chains, specifically mitochondrial electron transport chain enzymes. It exhibits low mammalian toxicity. In particular, it provides contact insecticidal effects against aphids, moths, scale insects, ants, and mosquitoes on vegetables and fruit trees, while not known to cause any harm to agricultural crops.

[0090] In this specific example, the pesticide composition comprises rotenone as an insecticidal ingredient and a composition for enhancing the efficacy of the aforementioned pesticide.

[0091] According to an exemplary specific example, the ratio (by weight) of rotenone in the pesticide composition: the composition for enhancing efficacy may be determined in a range of, for example, about 0.1 to 5:95 to 99.9, specifically about 0.2 to 1:99 to 99.8, more specifically about 0.3 to 0.8:99.2 to 99.7, and particularly specifically about 0.4 to 0.6:99.4 to 99.6.

[0092] In this regard, rotenone, which is used as a natural insecticide, is toxic to the human body (DL 50 : 132 mg / kg), it is desirable to achieve good insecticidal effect while reducing the content in the insecticide formulation as much as possible.

[0093] From this perspective, it is noteworthy that when a specific composition of an insecticide efficacy enhancer is used in combination with rotenone, as in the present specific example, an improved insecticidal effect can be achieved even with a relatively small amount of rotenone, which is an advantage not achieved in the prior art. In particular, in the case of an insecticide efficacy enhancer composition, the insecticidal activity is low when used alone, and when diluted alone to reduce the hazard of rotenone, the insecticidal activity may be insufficient.

[0094] On the other hand, even if a small amount of rotenone is used, as in the present specific example, when used in combination with a specific composition of an efficacy enhancer, an insecticidal effect significantly exceeding the normally expected level can be achieved, and at this time, the amount of rotenone used can also be reduced to a level that ensures safety, thereby achieving a complex effect.

[0095] In this way, the pesticide composition according to the present specific example can maximize the control effect while simultaneously minimizing environmental toxicity.

[0096] Meanwhile, according to an exemplary specific example, the agricultural pesticide composition may be a formulation in which at least one is selected from the group consisting of granules, powders, liquids, aerosols, sprays, extracts, pastes, fluid extracts, emulsions, suspensions, capsules, liquid wettable powders, granule wettable powders, wettable powders, powders, fine granules, oils, gel-type formulations, smokers, smoke screens, and fumigants.

[0097]

[0098] Uses of pesticide compositions

[0099] According to another specific example of the present disclosure, the pesticide composition described above can be sprayed to control agricultural pests present or potentially present in crops.

[0100] According to an exemplary specific example, the pesticide composition may be used as is, but typically, it may be appropriately diluted with water or the like, or sprayed directly on pests in an amount effective for controlling agricultural pests in a suspended form, or treated on crops or specific parts thereof (e.g., seeds, bulbs, rhizomes, etc.) where agricultural pests are expected to occur, or seeds or soil or carriers for sowing, or in the case of hydroponic cultivation, spraying (treating) directly on hydroponic solution.

[0101] In addition, it can include not only cases of spraying at the crop cultivation site, but also cases of spraying on seedbeds for seedlings, spraying before sowing, and cases of mixing it with soil after spraying on a carrier, etc.

[0102] According to an exemplary embodiment, the spraying method is not particularly limited, but in a specific embodiment, at least one spraying method selected from a heating misting method, a direct spraying (spraying) method, a drip spraying method, an immersion spraying method, etc. may be adopted.

[0103] The present invention can be more clearly understood by the following examples, which are provided merely for the purpose of illustrating the present invention and are not intended to limit the scope of the invention.

[0104]

[0105] Example

[0106]

[0107] A. Composition for enhancing the effectiveness of pesticides

[0108]

[0109] Preparation of a composition for enhancing the efficacy of pesticides

[0110] An efficacy enhancing composition was prepared according to the procedure illustrated in Fig. 1. Specifically, a natural lecithin emulsifier was melted and soybean oil was double-boiled. Thereafter, the melted lecithin emulsifier, double-boiled soybean oil, perilla extract (70% ethanol extract), and dill seed oil were mixed to prepare an insecticide efficacy enhancing composition having a composition of 70 wt% perilla extract, 15 wt% soybean oil, 10 wt% dill seed oil, and 5 wt% natural lecithin.

[0111]

[0112] Test for the presence or absence of phytotoxicity in pesticide efficacy enhancing compositions

[0113] The previously manufactured insecticide efficacy enhancing composition was visually examined for toxicity by comparing the 100-fold, 200-fold, and 500-fold effects of the previously manufactured insecticide efficacy enhancing composition on cucurbit and cucumber seedlings and the solanaceous seedlings, including peppers, with the untreated control group. The results are shown in Table 1 and Figure 2 below.

[0114]

[0115] Treatment details Crop toxicity level (0 to 5) 100 times Bell pepper 0 200 times 500 times 0 Untreated 0

[0116] Referring to the above table and drawings, it was confirmed that there was virtually no harm to the plants when the potency-enhancing composition was applied to bell peppers. This indicates that there is no harm to the plants themselves, even when the potency-enhancing composition is used alone.

[0117]

[0118] B. Insecticide composition

[0119]

[0120] Preparation of pesticide composition

[0121] Through the method illustrated in Fig. 2, an insecticide composition was prepared by combining the prepared efficacy-enhancing composition and rotenone in a weight ratio of 99.5:0.5.

[0122] The prepared insecticide composition was diluted 500-fold and tested for toxicity on cucumber and pepper (Solanaceae) seedlings, respectively. The results confirmed that treatment with the insecticide composition did not cause toxicity.

[0123]

[0124] Insecticidal effect test

[0125] The pesticide composition according to the example was used to test the control effect against pests such as aphids.

[0126] At this time, the pest control cost can be calculated using the following mathematical formula 1.

[0127]

[0128] [Mathematical Formula 1]

[0129] Pest control rate (%) = (Number of untreated insects - Number of treated insects) / Number of untreated insects × 100

[0130]

[0131] - The insecticidal effect was evaluated against tobacco whitefly (adult), cotton aphid, American silver beetle (nymph), rice water weevil, and peach aphid using a 500-fold dilution of the insecticide composition, and the results are shown in Figure 4.

[0132] According to the above figure, the killing rates for tobacco whitefly adults, cotton aphids, American silver beetle nymphs, rice water weevils adults, and peach aphids were 90.7%, 99.6%, 75.3%, 100%, and 96.4%, respectively.

[0133] - The results of evaluating the insecticidal effect against the American white moth (1-3 instar), the American white moth (4-5 instar), and the azalea beetle using a 500-fold dilution of the insecticide composition are shown in Figure 5.

[0134] Referring to the above figure, the American white moth showed a kill rate of 88.7% in the 1st-3rd instars and 71.4% in the 4th-5th instars. Meanwhile, the azalea beetle showed a kill rate of 93.7%.

[0135]

[0136] - The results of evaluating the insecticidal effect by the number of treatments when a 500-fold diluted insecticide composition was applied to mealybugs at 3-day intervals are shown in Figure 6.

[0137] Referring to the above drawing, the insecticidal rate did not reach 60% after one treatment, but increased rapidly after two treatments. However, no significant increase in insecticidal efficacy was observed after three treatments. Considering these results, it is believed that the insecticidal composition according to the present example can achieve maximum insecticidal effect with only about two or three sprays, and furthermore, can prevent problems related to pest resistance caused by frequent use of chemical agents.

[0138]

[0139] - The results of evaluating the insecticidal effect of two commercially available agents (Derris agent (commercially available D) and Pyrethrin agent (commercially available P)) and a 500-fold dilution of each insecticidal composition (SGI) according to an example on cotton aphids 3 days after treatment are shown in Fig. 7. Referring to the figure, the insecticidal rates of Derris agent and Pyrethrin agent against cotton aphids were 81.1% and 87.8%, respectively. On the other hand, the insecticidal composition according to an example showed an insecticidal rate close to 100%.

[0140] Meanwhile, the results of evaluating the insecticidal effect of two commercially available agents (Derris agent (commercially available D) and Pyrethrin agent (commercially available P)) and a 500-fold dilution of each insecticidal composition (SGI) according to an example on tobacco whitefly 3 days after treatment are shown in Fig. 8. Referring to the figure, the insecticidal rates of Derris agent and Pyrethrin agent against tobacco whitefly were 61.4% and 81.5%, respectively. On the other hand, the insecticidal composition according to an example showed an insecticidal rate of 90.7%.

[0141] The above results suggest that a synergistic effect was achieved among multiple components through the combination of rotenone and the potency-enhancing composition.

[0142]

[0143] Sterilization effect test

[0144] The insecticide composition according to the example was used to test the control effect against powdery mildew of melon. At this time, statistical analysis to verify the significant difference between the treatment groups' means was performed using SAS ver. 9.2 (SAS Institute, Cary, NC, USA) using Duncan's multiple range test (DMRT; P < 0.05). The significance between treatments was tested.

[0145] Meanwhile, the sterilization control value can be calculated using the following mathematical formula 2.

[0146]

[0147] [Equation 2]

[0148] Sterilization control rate (%) = (1 - outbreak rate in treatment group / outbreak rate in untreated group) × 100

[0149]

[0150] Table 2 below shows the results of the test for the control effect.

[0151]

[0152] Treatment details, outbreak rate, control agent, b (%), mildew (0-5), I repetition, II repetition, III repetition, average, a, outbreak start, 17.5, 15.0, 12.5, 15.0 ± 2.5, c, 7, 1.4, before outbreak, 28.8, 27.5, 16.3, 24.2 ± 6.9, b, 5, 3.9, no treatment, 57.5, 53.8, 46.3, 52.5 ± 5.7, a, C.V. (%), 16.8, 2

[0153] CV(Coefficient of variation, %) = Standard deviation / mean × 100

[0154]

[0155] According to the above table, the insecticide compositions treated with the examples showed a significant reduction in disease incidence compared to the untreated control group both at the onset and before outbreak, indicating a high control effect. Furthermore, virtually no phytotoxicity was observed in either the treated or untreated control groups.

[0156] All simple modifications or changes of the present invention fall within the scope of the present invention, and the specific scope of protection of the present invention will be made clear by the appended claims.

Claims

1. An agricultural pesticide composition comprising rotenone as an insecticide: a composition for enhancing the efficacy of an insecticide in a ratio of 0.1 to 3:97 to 99.9 by weight, At this time, the composition for enhancing the effectiveness of the pesticide is (i) 40 to 90 wt% of a perilla extract; (ii) 1 to 20 wt% of soybean oil; (iii) 1 to 30 wt% of dill seed oil; and (iv) 1 to 20 wt% of natural lecithin emulsifier; An insecticidal composition comprising:

2. An insecticide composition according to claim 1, characterized in that the extract of the plant is obtained using alcohol as an extraction solvent.

3. A pesticide composition, characterized in that in the second paragraph, the alcohol is at least one selected from the group consisting of ethanol, isopropanol, 1-propanol, isobutanol, n-butanol, butan-2-ol, and t-butanol.

4. A pesticide composition, characterized in that in the third paragraph, the alcohol is alcohol having a concentration of at least 30% (v / v).

5. In paragraph 1, the agricultural pesticide composition is characterized in that it has at least one formulation selected from the group consisting of granules, powders, liquids, aerosols, sprays, extracts, pastes, fluid extracts, emulsions, suspensions, capsules, liquid wettable powders, granule wettable powders, wettable powders, powders, granules, oils, gel-type preparations, smokers, smokescreens, and fumigants.

6. A step of spraying an agricultural pesticide composition according to any one of clauses 1 to 5; A method for killing agricultural pests, including:

7. A method for killing agricultural pests, characterized in that in paragraph 6, the spraying step is performed by at least one spraying method selected from the group consisting of a heating mist method, a direct spraying (spray) method, a drip spraying method, and an immersion spraying method.

8. A method for killing agricultural pests, characterized in that in paragraph 6, the agricultural pest is at least one selected from the group consisting of Lepidoptera, Hemiptera, Herbivores, Diptera, Hymenoptera, Ornithoptera, Thrips, Acari, Termites, Cockroaches and Flea orders.

9. A method for killing agricultural pests, characterized in that in paragraph 8, the agricultural pest is at least one selected from the group consisting of aphids, whiteflies, moths, shield beetles, weevils, cicadas, and scale insects.

10. A method for killing agricultural pests, characterized in that in paragraph 9, the aphids are at least one selected from the group consisting of cotton aphid, peach aphid, mulberry aphid, poplar aphid, sedge aphid, perilla aphid, and hairy aphid.

11. A method for killing agricultural pests, characterized in that in paragraph 9, the whitefly is at least one selected from the group consisting of greenhouse whiteflies and tobacco whiteflies.

12. A method for killing agricultural pests, characterized in that in paragraph 9, the moth species is at least one selected from the group consisting of the peach moth, the peach borer, the corn borer, the cutworm, the peach heart borer, the apple heart borer, the black-spotted moth, the gypsy moth, the tent moth, the pine moth, and the American white moth.

13. A method for killing agricultural pests, characterized in that in paragraph 9, the shield beetle is at least one selected from the group consisting of the Japanese maple shield beetle, the azalea shield beetle, the pear shield beetle, the water maple shield beetle, the oak shield beetle, and the cinnamon shield beetle.

14. A method for killing agricultural pests, characterized in that in paragraph 9, the cicada is at least one selected from the group consisting of a bride-winged cicada, a fan-winged cicada, a Japanese-winged cicada, a southern-winged cicada, a black-winged cicada, a black-tip cicada, a spotted cicada, a large spotted cicada, a black-spotted cicada, a large pointed cicada, a wavy pointed cicada, a white-striped yellow cicada, a white-spotted yellow cicada, a tip cicada, a two-spotted tip cicada, a black bear cicada, a brown-winged cicada, and a white-tailed cicada.

15. A method for killing agricultural pests, characterized in that in paragraph 9, the scale insects are at least one selected from the group consisting of maple scale insects, persimmon scale insects, horned mealybugs, turtle scale insects, mealybugs, brown scale insects, mulberry scale insects, maple scale insects, magnolia scale insects, and tangerine yellow scale insects.

16. A method for killing agricultural pests, characterized in that the agricultural pests in paragraph 6 are adults or larvae.

Images