Method for preparing herbal formulation having excellent Anti-inflammatory effect and herbal formulation prepared thereby

Abstract

The present invention relates to a method for preparing a herbal formulation having an excellent anti-inflammatory effect and a herbal formulation prepared thereby. The method for preparing a herbal formulation having an excellent anti-inflammatory effect of the present invention comprises: a herbal material preparation step (S100) of preparing herbal materials; a herbal material drying step (S200) of drying the prepared herbal materials to remove moisture; a herbal material pulverization step (S300) of pulverizing the dried herbal materials to prepare herbal material powder; a herbal material mixing step (S400) of mixing the herbal material powder with fructooligosaccharide at a predetermined weight ratio to prepare a mixed herbal powder; and a forming step (S500) of molding the mixed herbal powder into pills to produce the herbal formulation.

Description

Method for manufacturing a herbal medicine with excellent anti-inflammatory effect and herbal medicine manufactured thereby

[0001] The present invention relates to a method for manufacturing an herbal medicine with excellent anti-inflammatory effects and an herbal medicine manufactured thereby, and more particularly, to a method for manufacturing an herbal medicine with excellent anti-inflammatory effects, which exhibits excellent antioxidant and anti-inflammatory effects without causing harmful reactions to the human body and without cytotoxicity by forming a composition with natural materials such as indong powder, doraji powder, and burdock powder, and an herbal medicine manufactured thereby.

[0002]

[0003] Inflammation is characterized by a localized tissue response to invasion by, for example, microorganisms, specific antigens, damaged cells, or physical or chemical agents. The inflammatory response is a normally protective mechanism that not only initiates tissue repair but also serves to destroy, dilute, or sequester both the harmful agent and the damaged tissue. This inflammation can result from physical trauma, infection, some chronic diseases (e.g., autoimmune diseases such as psoriasis and rheumatoid arthritis), or chemical or physiological responses to external stimuli (e.g., as part of an allergic reaction).

[0004] Inflammatory mediators increase blood flow and local vasodilation, resulting in redness and heat, fluid exudation, and often local edema, leukocyte migration into the inflamed area, and pain. Many diseases are characterized by and caused by abnormal tissue-damaging inflammation. These conditions are typically characterized by the activation of immune defense mechanisms, which may result in more harmful than beneficial effects for the host, and are usually associated with varying degrees of tissue redness or hyperemia, swelling, fever, pain, pruritus, cell death, tissue destruction, cell proliferation, or loss of function. Examples include inflammatory bowel disease, rheumatoid arthritis, multiple sclerosis, psoriasis, glomerulonephritis, and transplant rejection.

[0005] These inflammatory changes are triggered by a series of cellular and biochemical events involving cells such as neutrophils, monocytes, macrophages and lymphocytes, along with inflammatory mediators such as vasoactive amines, cytokines, complement factors and reactive oxygen species. Above all, inflammation plays a crucial role in the wound healing process, and therefore, the occurrence of inflammation indicates that damage has occurred that needs to be healed.

[0006] Furthermore, while oxygen, which enters the body through respiration, has beneficial effects, this process also generates reactive oxygen species. Excessive reactive oxygen species expose oxygen to an unstable state, which can have detrimental effects on the animal body. Therefore, maintaining an appropriate level of reactive oxygen species is considered a key principle in preventing cellular oxidation and aging, and research on antioxidants is continuously ongoing.

[0007] In consideration of the above, continuous research on substances with antioxidant and anti-inflammatory effects has been continued, and various solutions have been proposed for them. For example, Korean Patent Publication No. 10-2016-0018496 discloses a natural composition with antioxidant and anti-inflammatory effects, titled "A natural composition with antioxidant and anti-inflammatory effects, extracted by mixing and extracting Angelica gigas, Sophora japonica, Oyster, Red peony, Cimicifuga chinensis, Prunus persica, Safflower, Licorice, and Cinnamon, wherein 100 parts by weight of Angelica gigas, 50 to 80 parts by weight of Sophora japonica, 50 to 80 parts by weight of Oyster, 50 to 80 parts by weight of Red peony, 50 to 80 parts by weight of Cimicifuga chinensis, 30 to 70 parts by weight of Prunus persica, 30 to 70 parts by weight of Safflower, 20 to 50 parts by weight of Licorice, and 20 to 50 parts by weight of Cinnamon are mixed and extracted."

[0008] In addition, Korean Patent Publication No. 10-2016-0098569 discloses an anti-inflammatory and antioxidant pharmaceutical composition and food composition containing an extract of Eriocaulon decemflorum, which is an "anti-inflammatory composition containing an extract of Eriocaulon decemflorum, wherein the extract of Eriocaulon decemflorum is extracted using a cold steeping method with any one selected from the group consisting of alcohols having 1 to 5 carbon atoms, hexane, chloroform, ethanol, water, and mixtures thereof."

[0009] In addition, Korean Patent Publication No. 10-2018-0021012 discloses a "natural composition with antioxidant and anti-inflammatory effects, extracted by mixing and extracting Angelica gigas, Sonamul, Oyak, Red Peony, Hyangbuja, Dosin, Safflower, Licorice, Cinnamon, Evening Primrose, Willow, and Tangerine fruit" under the name of a natural composition with antioxidant and anti-inflammatory effects.

[0010] However, although the inventions of the above-mentioned patent documents may exhibit antioxidant and anti-inflammatory effects, it is not known whether they can exhibit anti-inflammatory effects while being non-cytotoxic and, furthermore, exhibit antibacterial effects at a satisfactory level. Therefore, it is still necessary to extract effective ingredients from natural substances that exhibit excellent antioxidant and anti-inflammatory effects as described above while not exhibiting cytotoxicity to the human body, and to provide a composition for inhibiting antioxidant and anti-inflammatory effects therefrom.

[0011]

[0012] The problem to be solved by the present invention is to provide a method for manufacturing an herbal medicine with excellent anti-inflammatory effects, which exhibits excellent antioxidant and anti-inflammatory effects without causing harmful reactions to the human body and without cytotoxicity, by forming a composition with natural materials such as indong powder, doraji powder, and burdock powder, and an herbal medicine manufactured thereby.

[0013] The various problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below.

[0014]

[0015] In one embodiment of the technical idea of ​​the present invention, a method for manufacturing a herbal medicine having excellent anti-inflammatory effect is disclosed.

[0016] The method for manufacturing the herbal medicine preparation having excellent anti-inflammatory effect includes a herbal medicine preparation step (S100) of preparing herbal medicine ingredients; a herbal medicine drying step (S200) of drying the prepared herbal medicine ingredients to remove moisture; a herbal medicine grinding step (S300) of grinding the dried herbal medicine ingredients to produce herbal medicine ingredient powder; a herbal medicine mixing step (S400) of mixing the herbal medicine ingredient powder and fructooligosaccharide at a predetermined weight ratio to produce a herbal medicine ingredient mixture powder; and a pill making step (S500) of forming the herbal medicine ingredient mixture powder into a pill to produce a herbal medicine preparation.

[0017] In the above herbal medicine ingredient preparation step (S100), the herbal medicine ingredients may include indong, doraji, burdock, Ophiopogon japonicus, wolfberry, ginseng, buckwheat, dokkomari fruit, magnolia berry, ginger, peppermint, and green tea.

[0018] In the above herbal medicine ingredient mixing step (S400), the herbal medicine ingredient mixture powder may be included in a weight ratio of 18 to 22 parts by weight of indigo powder, 12 to 17 parts by weight of doraji powder, 8 to 12 parts by weight of burdock powder, 3 to 7 parts by weight of Ophiopogon japonicus powder, 3 to 7 parts by weight of wolfberry powder, 3 to 7 parts by weight of ginseng powder, 3 to 7 parts by weight of buckwheat powder, 3 to 7 parts by weight of cocklebur fruit powder, 3 to 7 parts by weight of omija powder, 3 to 7 parts by weight of dried ginger powder, 3 to 7 parts by weight of peppermint powder, 3 to 7 parts by weight of green tea powder, and 3 to 7 parts by weight of fructooligosaccharide.

[0019] In addition, in another embodiment of the technical idea of ​​the present invention, an herbal medicine having excellent anti-inflammatory effect prepared by the above method is disclosed.

[0020] Specific details of other embodiments are included in the detailed description.

[0021]

[0022] The method for manufacturing an herbal medicine with excellent anti-inflammatory effects according to various embodiments of the technical idea of ​​the present invention includes natural materials such as indong powder, doraji powder, and burdock powder, thereby manufacturing an herbal medicine with excellent antioxidant and anti-inflammatory effects without causing harmful reactions to the human body and without cytotoxicity.

[0023] It will be fully understood that embodiments of the technical idea of ​​the present invention can provide various effects not specifically mentioned.

[0024]

[0025] Figure 1 is a flowchart illustrating a method for manufacturing a herbal medicine with excellent anti-inflammatory effect according to one embodiment of the technical idea of ​​the present invention.

[0026] Figure 2 is a microscopic image and graph showing the establishment of a cell model that induces inflammation by LPS.

[0027] FIG. 3 is a graph showing cytotoxicity evaluation according to a water extract (DW extract, DE) of a herbal medicine powder according to one embodiment of the technical idea of ​​the present invention.

[0028] FIG. 4 is a graph showing cytotoxicity evaluation according to an ethanol extract (EtOH extract, EE) of a herbal medicine powder according to one embodiment of the technical idea of ​​the present invention.

[0029] Figure 5 is a graph showing the evaluation of DE anti-inflammatory efficacy at the mRNA level in LPS-induced inflammatory response.

[0030] Figure 6 is a graph showing the evaluation of EE anti-inflammatory efficacy at the mRNA level in LPS-induced inflammatory response.

[0031] FIG. 7 is a graph showing the inhibitory effect of a herbal medicine powder extract on nitric oxide production in RAW 264.7 cells according to one embodiment of the technical idea of ​​the present invention.

[0032] Figure 8 is a graph showing the inhibition of LPS-induced inflammatory cytokine secretion by a herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention.

[0033] Figure 9 is a graph showing the anti-inflammatory control mechanism of a herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention in an LPS-induced inflammatory response.

[0034]

[0035] The advantages and features of the present invention, and the methods for achieving them, will become clearer with reference to the embodiments described in detail below. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided to ensure that the disclosure is thorough and complete and to sufficiently convey the spirit of the present invention to those skilled in the art.

[0036] The terminology used in this application is solely for the purpose of describing specific embodiments and is not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise.

[0037] Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by those of ordinary skill in the art to which this invention pertains. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with their meaning in the context of the relevant technology, and shall not be construed in an idealized or overly formal sense unless explicitly defined herein.

[0038]

[0039] Hereinafter, with reference to the attached drawings, a preferred embodiment of a method for manufacturing a herbal medicine with excellent anti-inflammatory effect according to an embodiment of the technical idea of ​​the present invention will be described in detail.

[0040]

[0041] Figure 1 is a flowchart illustrating a method for manufacturing a herbal medicine with excellent anti-inflammatory effect according to one embodiment of the technical idea of ​​the present invention.

[0042]

[0043] Referring to FIG. 1, a method for manufacturing an herbal medicine having an excellent anti-inflammatory effect according to one embodiment of the technical idea of ​​the present invention includes a step of preparing herbal medicine ingredients (S100), a step of drying herbal medicine ingredients (S200), a step of crushing herbal medicine ingredients (S300), a step of mixing herbal medicine ingredients (S400), and a step of making pills (S500).

[0044]

[0045] 1. Preparation of herbal medicine ingredients (S100)

[0046] The above herbal medicine material preparation step (S100) is a step for preparing herbal medicine materials for manufacturing herbal medicine preparations.

[0047] In the above herbal medicine ingredient preparation step (S100), the herbal medicine ingredients may include indong, doraji, burdock, Ophiopogon japonicus, wolfberry, ginseng, buckwheat, dokkomari fruit, magnolia berry, ginger, peppermint, and green tea.

[0048]

[0049] The honeysuckle (Lonicera japonica) is a vine plant of the Caprifoliaceae family, a dicotyledonous plant. Since its leaves do not fall off in some places even in winter, it is called "rendong" (evergreen winter). In traditional Chinese medicine, the leaves and stems of honeysuckle are called "rendongteng" (honeysuckle vine), and its flower buds are called "jinyinhua" (gold and silver flower) because white and yellow flowers can be seen simultaneously. It is used not only for treating boils and sores but also for asthma, bronchitis, etc.

[0050] The components of honeysuckle vine mainly include flavonoids such as luteolin and lonicerin, which are flavonoid compounds of the Scrophulariaceae family, chlorogenic acid, and isochlorogenic acid. Volatile oils such as linalool and cis-2,6,6-trimethyl-2-ethenyl-5-hydroxy-tetrahydropyrane account for approximately 0.6%. In addition, it contains trace amounts of loganin, inositol, alcohol, tannin, saponin, etc. In particular, according to recent research results, the honeysuckle flower contains isosides and saponin components, and it is reported to have an inhibitory effect on various bacteria including pneumonia diplococcus, as well as effects on various pyogenic infections and liver toxicity protection.

[0051]

[0052] The above-mentioned Platycodon grandiflorum grows widely in the mountainous regions of Korea, Japan, and China, and is a wild vegetable widely used as a medicinal herb in oriental medicine and as a general food, containing triterpenoid saponins, carbohydrates, and fiber. These saponins have been found to have antitussive, expectorant, central nervous system suppressing, acute and chronic inflammation, antiulcer and gastric juice secretion suppressing effects, hypoglycemic effects that lower blood pressure by dilating blood vessels, cholesterol metabolism improving effects, antioxidant and anticancer effects, etc. In addition, the number of herbal prescriptions containing Platycodon grandiflorum as a herbal medicine is recorded in 287 cases in Donguibogam and 49 cases in Bangyakhappyeon, showing that it has a variety of pharmacological effects.

[0053] The above-mentioned doraji is not only good for coughs, colds, and asthma, but it also suppresses the production of melanin pigments, making the skin white and smooth, and it helps to remove waste products, promotes blood circulation, and has anti-inflammatory properties, making it effective in treating acne.

[0054] Additionally, the roots of the Platycodon grandiflorum contain approximately 2% saponin, which has hemolytic properties, relieves coughs and phlegm, reduces inflammation, lowers blood sugar, and lowers cholesterol. Furthermore, it is rich in calcium, which is beneficial for preventing osteoporosis and supporting bone health in growing children.

[0055]

[0056] The above burdock contains a lot of calcium, which helps prevent osteoporosis and maintain bone health. It is rich in vitamin B, which helps prevent aging, eye fatigue, and the immune system. It contains saponins, inulin, and arginine, which help strengthen the immune system and have antioxidant effects. The lignin component contained in it helps intestinal health by adsorbing carcinogens in the intestines and excreting them from the body. It is also rich in inulin, a polysaccharide, which helps improve and prevent diabetes. It is known to contain a large amount of ingredients that are effective for the human body.

[0057]

[0058] The above-mentioned Liriope platyphylla is a perennial herbaceous plant belonging to the lily family that grows well in shady, moist places, blooms purple flowers in July, and bears black fruits in October. Its close relatives include Ophiopogon japonicus and Liriope spicata. The efficacy of Liriope platyphylla has been reported to include hypoglycemic action, anti-inflammatory action, IgM antibody production inhibition action, anti-arrhythmic action, anticancer action of butanol fraction, and action as an antagonist for leukopenia. Liriope platyphylla, which is known to have various pharmacological effects, refers to the swollen part of the tuberous root of Liriope platyphylla, and it has been generally used by digging it up, peeling it, and drying it.

[0059]

[0060] The above wolfberry (Lycium chinense) is the fruit of the wolfberry tree, which belongs to the Solanaceae family. The wolfberry is a round or oval berry with a wrinkled exterior when dried and many seeds inside. The components of wolfberry include betaine, zeazantin, carotene, thiamine, vitamins A, B1, B2, C, physalien, and β-sistosterol, as well as various inorganic substances, and it has the effect of strengthening the body and reducing blood pressure. It is effective in protecting the liver by suppressing and lowering the intestinal absorption of low-density cholesterol, and it has excellent preventative and therapeutic effects on adult diseases such as hypertension, angina pectoris, arteriosclerosis, and diabetes, and is also known to have an excellent effect of protecting eyesight.

[0061]

[0062] The above ginseng (Panax ginseng C.A. Meyer) has been known as the best herbal health food for over 2,000 years. The main physiologically active substances of ginseng include ginseng saponin and phenolic substances, and in particular, ginseng saponin has been revealed through various research results to have effects such as anti-diabetic, anti-cancer, antioxidant, arteriosclerosis and hypertension prevention, liver function promotion, hangover relief, anti-fatigue and anti-stress, anti-inflammatory activity, allergic disease treatment, and protein synthesis promotion. In addition, phenolic substances, another component of ginseng, are representative antioxidant substances and can be used in various fields such as the food, pharmaceutical, and cosmetic industries due to their potential for disease treatment.

[0063]

[0064] The above-mentioned Houttuynia cordata is a perennial herb of the family Trifoliaceae, and is the only species in the monotypic genus Houttuynia cordata. It is also called Houttuynia cordata because its leaves and stems emit a meaty odor. It has various names such as Jungyakcho, Jupchae, and Sipyak. Houttuynia cordata is known to be effective in treating skin diseases such as antibacterial, skin health, acne, and eczema, and is also known to help with hair health. The Houttuynia cordata of the present invention uses various organs or parts of Houttuynia cordata (e.g., leaves, flowers, roots, stems, etc.) and the middle plant.

[0065] The whole plant of the above-mentioned medicinal herb contains 0.0049% of essential oil components, which include decanoyl acetaldehyde, myrcene, lauric aldehyde, and capric aldehyde. Medicinal herb is excellent at removing various harmful inflammations and waste products from the body, and is excellent at excreting various harmful substances and heavy metals that have accumulated in the body due to fine dust.

[0066] Quercitrin, found abundantly in buckwheat, dilates capillaries, helping to remove inflammation and impurities from the scalp. This promotes scalp health and significantly alleviates hair loss symptoms. Furthermore, quercitrin possesses excellent antibacterial and anti-inflammatory properties, making it effective in improving blemishes, acne, and various skin problems. It also aids in anticancer activity, blood circulation, and diuresis, and is known as a natural product that is beneficial for detoxification and bronchial health.

[0067]

[0068] The above-mentioned cocklebur (Xanthium Strumarium) is an annual herb, about 1 m tall, and has hairs on both the leaves. The leaves are alternate, with long petioles and 5 to 10 cm in length. The flowers bloom from August to September, are yellow, and grow in a cone-shaped pattern at the ends of branches and stems. There are male and female flowers, and the capitulum of the male flower is round and grows at the end, while the capitulum of the female flower grows at the base and has two protrusions. The fruit ripens in September, and the achenes have hook-like protrusions on the outside that help them stick to objects.

[0069] The above-mentioned cocklebur fruit was mainly used to make antiseptic and antiseptic ointments for eczema, lichen, blemishes, boils, boils, smallpox, and ulcerative dermatitis (Korean Resource Plants, Seoul National University Press, 1996). The effective ingredients of cocklebur include xanthostrumain (yellow glycoside), resin, iodine salt, saponin, and xanthin fatty acids, and the fatty acids are composed of linoleic acid, oleic acid, saturated fatty acids, palmitic acid, and stearic acid (Components and Uses of Medicinal Herbs, Ilwolseogak, 1996; Korean Resource Plants, Seoul National University Press, 1996; Dictionary of Chinese Medicine, Shogakukan, Japan, 1985).

[0070]

[0071] The above-mentioned Schizandra chinensis Baillon is a deciduous broadleaf vine with leaves that grow alternately, ovate or obovate, acutely pointed at the tip, serrated, and somewhat hairy on the veins on the back. The flowers are reddish-white, bloom in June and July, and are dioecious. The fruit is called Schizandra chinensis (Omaeja) or Schizandrae fructus. Its main producing areas are Jirisan Mountain, North Jeolla Province, and Gangwon Province at elevations of 200-1,600m, and it grows wild throughout the country except for South Chungcheong Province and North Chungcheong Province. It is geographically distributed in Japan, Sakhalin, Manchuria, and China.

[0072] The fruit of the above-mentioned Schizandrol contains Schizandrin A, B, C, angeloylgomisin O, P, epigomisin, pregomoisin, and deoxyschizandrin. Schizandrin has long been known to have medicinal properties such as astringent, nourishing, strengthening, relieving cough, relieving toxins, relieving thirst, astringent, ripening phlegm, and toning the body and mind.

[0073]

[0074] Ginger (Zingiber officinale) is a perennial plant belonging to the genus Zingiber in the family Zingiberaceae. Its rhizome is used as a traditional herbal medicine and has a pungent, bitter flavor. Its aroma and pungent flavor are believed to be derived from a component called gingerol, which is known to have excellent antioxidant and anti-inflammatory properties.

[0075]

[0076] The above peppermint (Mentha arvensis var. piperascens) is a perennial herb of the Lamiaceae family, which has an aromatic nature and grows upright with branches at the top. The above peppermint contains active ingredients such as menthol, isomenthone, pinene, camphene, limonene, zingerone, and shogaol, which give off a refreshing and cool scent, and has bactericidal and antibacterial effects against pathogenic fungi that cause eczema and scabies on the skin and scalp, as well as microorganisms such as Escherichia coli and Staphylococcus aureus. In particular, it can suppress the growth and activity of a microorganism called Pityrosporum Ovale, which is the main cause of aggravating dandruff and itching, and has the effect of relieving irritation, erythema, and seborrheic inflammation (Korean Patent No. 448,434).

[0077]

[0078] The green tea leaves are 75-80% water, with the remainder being solids, containing polyphenol compounds (flavanols, flavandiols, flavonoids, phenolic acids). Most of these polyphenol compounds are commonly known as catechins. Additionally, they contain various vitamins, alkaloids (e.g., caffeine), pigments, 28 types of amino acids including theanine and glutamic acid, minerals, various enzymes (e.g., polyphenoloxidase), organic acids, and carbohydrates.

[0079] The above green tea contains more catechin compounds than general plants, and is related to various physiological activities unlike general plants. Up to now, various research results on green tea catechin compounds have been reported, and in particular, anticancer, antioxidant, antibacterial, antiviral, anti-allergy, blood pressure increase suppression, blood cholesterol lowering, and caries prevention have been reported.

[0080]

[0081] 2. Drying stage of herbal medicine ingredients (S200)

[0082] The above herbal medicine material drying step (S200) is a step of drying the prepared herbal medicine material to remove moisture.

[0083] In the above-mentioned herbal medicine material drying step (S200), the prepared herbal medicine material is dried to remove moisture remaining in the herbal medicine material, thereby improving the texture and flavor of the herbal medicine preparation and enhancing the storage and preservation properties. The drying of the prepared herbal medicine material may be performed through a process of primary drying and vacuum drying.

[0084] For example, the primary drying can evenly remove moisture inside the prepared herbal medicine material by drying the prepared herbal medicine material at a temperature of 25 to 35°C for 1 to 5 days.

[0085] In addition, the vacuum drying can be performed by irradiating microwaves to the primary dried herbal medicine material in a reduced pressure atmosphere. Specifically, the vacuum drying can be performed by irradiating microwaves of 2.0 to 2.5 GHz to the primary dried herbal medicine material for 1 to 30 minutes at a pressure of 50 to 60 mbar and a temperature of 35 to 45°C.

[0086] In the above vacuum drying, microwaves can penetrate into the interior of the primary dried herbal material and exhibit a volumetric heating effect that heats the entire herbal material. When the microwaves are irradiated, water molecules inside the herbal material vibrate or rotate according to the polarity conversion of the microwaves, and this polarization vibration leads to friction between molecules, causing a heat generation phenomenon. As a result, microwaves can penetrate into the herbal material in a short period of time without heat conduction and are converted into heat due to friction caused by the polarity conversion, thereby increasing the heating efficiency.

[0087] In addition, in the present invention, when microwaves are irradiated under reduced pressure, expansion occurs due to heating inside the herbal medicine material, and a sterilizing effect can also be generated by destroying cell membranes such as layers and stratification due to this instantaneous internal heating and expansion.

[0088]

[0089] 3. Herbal medicine material grinding stage (S300)

[0090] The above herbal medicine material grinding step (S300) is a step of grinding the dried herbal medicine material to produce herbal medicine material powder.

[0091] That is, in the above herbal medicine material grinding step (S300), the dried herbal medicine material can be ground using a known grinder to produce herbal medicine material powder. Specifically, the herbal medicine material powder can include indong powder, doraji powder, burdock powder, Ophiopogon japonicus powder, wolfberry powder, ginseng powder, yakmomil powder, dokkomari fruit powder, omija powder, dried ginger powder, peppermint powder, and green tea powder.

[0092]

[0093] Meanwhile, in a method for manufacturing a herbal medicine preparation according to another embodiment of the technical idea of ​​the present invention, instead of manufacturing a herbal medicine powder by grinding the dried herbal medicine material in the herbal medicine material grinding step (S300), a composition may be included for manufacturing a fermented herbal medicine powder by fermenting and then grinding the dried herbal medicine material, and using the fermented herbal medicine powder to manufacture a herbal medicine preparation.

[0094] That is, in the method for manufacturing a herbal medicine according to another embodiment of the technical idea of ​​the present invention, the fermented herbal medicine powder may be manufactured by mixing 10 to 20 parts by weight of a lactic acid bacteria culture medium and 5 to 15 parts by weight of sugar with respect to 100 parts by weight of the total content of the dried herbal medicine, fermenting the mixture at a temperature of 4 to 36°C for 2 to 8 days, and grinding the fermented herbal medicine using a known grinder.

[0095] The lactic acid bacteria used in the above lactic acid bacteria culture solution may be at least one selected from the group consisting of strains of the genus Lactobacillus, genus Lactococcus, genus Leuconostoc, genus Pediococcus, and genus Bifidobacterium.

[0096] For example, the lactic acid bacteria may be a strain of the genus Lactobacillus, and the strain of the genus Lactobacillus may be at least one strain selected from the group consisting of Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus sakei, Lactobacillus casei, and Lactobacillus brevis.

[0097] Specifically, it is preferable that the lactic acid bacteria is a mixed strain in which Lactobacillus plantarum, Lactobacillus casei, and Lactobacillus sakei are mixed in a weight ratio of 1:1:1.

[0098] In addition, the above lactic acid bacteria culture solution is cultured at 38 to 42°C for 25 to 35 hours after inoculating lactic acid bacteria in a culture medium, and then 4×1.08 to 6×1.0 8 A lactic acid bacteria culture dilution solution is prepared by diluting to CFU / mL, and then centrifuging the lactic acid bacteria culture dilution solution at 20,000 rpm for 10 to 20 minutes to separate only the supernatant located at the top, and filtering the supernatant with a 0.5 μm syringe filter.

[0099] In the above lactic acid bacteria culture solution, the inoculation amount of the lactic acid bacteria is 1×1.0 8 ~ 1×1.0 8 It may be CFU / mL, and the culture medium includes glucose, dextrin, glycerin, sodium citrate, malt extract, magnesium chloride and peptone. Specifically, the culture medium may include 20 g / L of glucose, 10 g / L of dextrin, 20 g / L of glycerin, 5 g / L of sodium citrate, 20 g / L of malt extract, 3 g / L of magnesium chloride and 8 g / L of peptone per 1 liter (L) of purified water.

[0100] In addition, the above-mentioned sugar can function as food for lactic acid bacteria. For example, the above-mentioned sugar can be used as one or more selected from the group consisting of sugar, oligosaccharide, glucose, and maltose. Preferably, glucose and sugar can be used in a mixture at a weight ratio of 1:1.

[0101]

[0102] 4. Mixing of herbal ingredients (S400)

[0103] The above herbal medicine material mixing step (S400) is a step of manufacturing a herbal medicine material mixed powder by mixing the above herbal medicine material powder and fructooligosaccharide at a certain weight ratio.

[0104] In the above herbal medicine ingredient mixing step (S400), the herbal medicine ingredient mixture powder may be included in a weight ratio of 18 to 22 parts by weight of indigo powder, 12 to 17 parts by weight of doraji powder, 8 to 12 parts by weight of burdock powder, 3 to 7 parts by weight of Ophiopogon japonicus powder, 3 to 7 parts by weight of wolfberry powder, 3 to 7 parts by weight of ginseng powder, 3 to 7 parts by weight of buckwheat powder, 3 to 7 parts by weight of cocklebur fruit powder, 3 to 7 parts by weight of omija powder, 3 to 7 parts by weight of dried ginger powder, 3 to 7 parts by weight of peppermint powder, 3 to 7 parts by weight of green tea powder, and 3 to 7 parts by weight of fructooligosaccharide.

[0105] In the above herbal medicine material mixing step (S400), the fructooligosaccharide is a type of functional oligosaccharide that is not only effective in improving the physical properties of food but also has health-beneficial properties, and refers to an oligosaccharide in which 1 to 3 fructose molecules are β-(2,1) bonded to sucrose, and mixed as 1-kestose (1-Kestose GF2), nystose (Nystose GF3), or fructosyl nystose (Fructosyl nystose GF4).

[0106] Specifically, the fructooligosaccharide may be at least one selected from the group consisting of 1-kestose, nystose, and fructosyl nystose.

[0107]

[0108] 5. Refund Stage (S500)

[0109] The above-mentioned pill preparation step (S500) is a step of manufacturing a herbal medicine preparation by forming the above-mentioned herbal medicine mixed powder into a pill.

[0110] In the above-mentioned pill making step (S500), the herbal medicine powder mixture can be made into a pill using a known pill making machine. The size of the pill can be made to have a diameter of 3 to 4 mm so as to be suitable for ingestion.

[0111] The configuration of forming a mixture of herbal medicine powder into a pill using a known pill making machine in the above pill making step (S500) is a known technology, and for the convenience of explanation and clarity of the technical idea of ​​the present invention, a detailed description thereof will be omitted.

[0112]

[0113] Hereinafter, with reference to the attached drawings, a specific example of a method for manufacturing a herbal medicine with excellent anti-inflammatory effect according to an embodiment of the technical idea of ​​the present invention will be described in more detail.

[0114]

[0115] < Example >

[0116] First, herbal medicine ingredients consisting of indong, doraji, burdock, Ophiopogon japonicus, wolfberry, ginseng, yakmomil, dokkomari fruit, omija, ginger, peppermint, and green tea were prepared, and then the prepared herbal medicine ingredients were dried to remove moisture.

[0117] At this time, the drying of the prepared herbal medicine material was carried out through the process of primary drying and vacuum drying. The primary drying was performed by drying the prepared herbal medicine material at a temperature of 30°C for 3 days, and the vacuum drying was performed by irradiating the primary dried herbal medicine material with microwaves of 2.3 GHz at a pressure of 55 mbar and a temperature of 40°C for 10 to 20 minutes depending on the herbal medicine material.

[0118] Next, the dried herbal ingredients were ground to produce powdered indong, doraji, burdock, Ophiopogon japonicus, wolfberry, ginseng, buckwheat, cocklebur, magnolia berry, dried ginger, peppermint, and green tea.

[0119] Next, a mixed powder of herbal medicine ingredients was prepared by mixing 20 parts by weight of the above-mentioned indong powder, 15 parts by weight of the above-mentioned doraji powder, 10 parts by weight of the above-mentioned burdock powder, 5 parts by weight of the above-mentioned Ophiopogon japonicus powder, 5 parts by weight of the above-mentioned wolfberry powder, 5 parts by weight of the above-mentioned ginseng powder, 5 parts by weight of the above-mentioned buckwheat powder, 5 parts by weight of the above-mentioned cocklebur fruit powder, 5 parts by weight of the above-mentioned magnolia berry powder, 5 parts by weight of the above-mentioned dried ginger powder, 5 parts by weight of the above-mentioned peppermint powder, 5 parts by weight of the above-mentioned green tea powder, and 5 parts by weight of the above-mentioned fructooligosaccharide.

[0120] Next, the above herbal medicine mixture powder was molded into a pill with a diameter of 3.5 mm to manufacture a herbal medicine preparation.

[0121]

[0122] Experiment

[0123] The anti-inflammatory effect of a herbal medicine powder according to one embodiment of the technical idea of ​​the present invention was tested.

[0124] In one embodiment of the technical idea of ​​the present invention, the herbal medicine powders were mixed in a weight ratio of 20 parts by weight of indigo powder, 15 parts by weight of doraji powder, 10 parts by weight of burdock powder, 5 parts by weight of Ophiopogon japonicus powder, 5 parts by weight of wolfberry powder, 5 parts by weight of ginseng powder, 5 parts by weight of buckwheat powder, 5 parts by weight of cocklebur fruit powder, 5 parts by weight of magnolia berry powder, 5 parts by weight of dried ginger powder, 5 parts by weight of peppermint powder, and 5 parts by weight of green tea powder.

[0125] (1) Characteristics of Raw 264.7 cells

[0126] a. Macrophage origin: Raw 264.7 cells are mouse-derived macrophages, a cell type that plays a crucial role in the human immune system. Macrophages are crucial for recognizing and eliminating pathogens and serve as key regulators of the inflammatory response.

[0127] b. Description of the inflammatory response: These cells produce inflammatory cytokines (e.g., TNF-α, IL-6, and IL-1β) and other inflammatory mediators. This makes them an ideal model for studying inflammatory responses.

[0128] High responsiveness: Raw 264.7 cells are more sensitive to various stimuli than other macrophages, which allows the study of inflammatory pathways and their regulation.

[0129] (2) Role of Lipopolysaccharide (LPS)

[0130] a. Inflammatory agent: Lipopolysaccharide (LPS), a molecule found in the outer membrane of Gram-negative bacteria, is a potent inducer of inflammation. LPS activates the inflammatory response by binding to Toll-like receptor 4 (TLR4) on macrophages.

[0131] b. Activation of immune response: LPS can increase the production of inflammatory cytokines by activating the NF-κB pathway, which describes a key part of the inflammatory process.

[0132] A. Standardized approach: Induction of inflammation using LPS has been widely used in several recent studies and is useful for comparing and standardizing results across studies.

[0133] (3) Research Application

[0134] a. Search for anti-inflammatory agents: Using Raw 264.7 cells and LPS, we can induce an inflammatory response and evaluate whether various compounds or extracts suppress this response.

[0135]

[0136] < Results >

[0137] 1. Establishment of a cell model for inflammation induction by LPS

[0138] Figure 2 is a microscopic image and graph showing the establishment of a cell model that induces inflammation by LPS.

[0139] Figure 2(A) shows a comparative analysis of the microscopic images of the control group (CTL) and the experimental group treated with LPS over time, set at 0, 24, 48, and 72 hours, and the morphological changes in the cells can be clearly observed.

[0140] Figure 2(B) shows the results of a comparison of growth rates based on the area occupancy ratio of cells. It can be seen that the area occupancy ratio increases in the LPS treatment group, which may reflect a morphological change in the cells compared to the control group.

[0141] Figure 2(C) shows that the LPS-treated group showed a reduced cell number compared to the control group in an analysis based on cell number. When microscopic images were compared and analyzed together, it can be assumed that this result is related to morphological changes and possible cell death of cells caused by LPS.

[0142] Figure 2(D) shows the results measured via qPCR, showing that the mRNA expression levels of tumor necrosis factor (TNF), interleukin-6 (IL-6), interleukin-1β (IL-1β), and inducible nitric oxide synthase (iNOS) genes significantly increased in the LPS-treated group compared to the control group. This suggests that LPS induced an inflammatory response within cells and established an inflammation-induced cell model.

[0143]

[0144]

[0145] 2. Toxicity assessment of raw materials on macrophage proliferation

[0146] Water and ethanol have different properties as extraction solvents, which greatly affect the components obtained during the extraction process. Water is a highly polar solvent and can effectively dissolve water-soluble compounds such as sugars, amino acids, peptides, and some vitamins.

[0147] On the other hand, ethanol has moderate polarity and is suitable for the extraction of polar substances as well as nonpolar compounds such as lipids, natural pigments, certain alkaloids, and essential oils that are insoluble in water.

[0148] Based on these differences, the herbal medicine powder according to one embodiment of the technical idea of ​​the present invention was extracted into water extract (DW extract, DE) and ethanol extract (EtOH extract, EE), respectively, and the toxicity and anti-inflammation of the raw material on the LPS inflammation-induced cell model were evaluated.

[0149]

[0150] FIG. 3 is a graph showing cytotoxicity evaluation according to a water extract (DW extract, DE) of a herbal medicine powder according to one embodiment of the technical idea of ​​the present invention.

[0151]

[0152] The effects of the water extract (DW extract, DE) of the herbal medicine powder according to one embodiment of the technical idea of ​​the present invention on cell proliferation and toxicity were evaluated using an established LPS-induced cell model (see FIG. 2) (see FIG. 3).

[0153]

[0154] DE was treated with serial dilutions of concentrations from 0 μg / ml (control) to 1000 μg / ml, and after treatment with LPS, the area occupancy ratio and cell number of cells were measured for 72 hours, and cell images were taken after 0 hours, 24 hours, 48 ​​hours, and 72 hours.

[0155]

[0156] Referring to Fig. 3, the DE treatment group showed an effect of increasing the proliferation of Raw 264.7 cells at all concentrations compared to the control group (24.45%, 787.6 cells), but the maximum growth rate (55.95%, 1491 cells) was observed at 500 ㎍ / ml, whereas the cell growth rate decreased at 1000 ㎍ / ml (32.42%, 838 cells), suggesting that DE at this concentration may induce cytotoxicity.

[0157]

[0158] When DE and LPS were treated together, morphological changes due to LPS appeared, and the cell growth rate was significantly reduced compared to the group treated with DE alone (20.41%, 425.8 cells). The area occupancy ratio was higher than that of the control group at a concentration of 15.63 ㎍ / ml (26.16%, 490.3 cells), and the cell number was higher than that of the control group at a concentration of 3.91 ㎍ / ml (23.27%, 573.3 cells).

[0159]

[0160] FIG. 4 is a graph showing cytotoxicity evaluation according to an ethanol extract (EtOH extract, EE) of a herbal medicine powder according to one embodiment of the technical idea of ​​the present invention.

[0161]

[0162] The cell proliferation effect and toxicity of the ethanol extract (EE) of the herbal medicine powder according to one embodiment of the technical idea of ​​the present invention were evaluated using an established LPS-induced cell model (see Fig. 2).

[0163]

[0164] After treatment with EE diluted to various concentrations from 0 ㎍ / ml (control) to 1000 ㎍ / ml, the area occupancy ratio and cell number of cells were measured for 72 hours, and cell images were taken after 0 hours, 24 hours, 48 ​​hours, and 72 hours.

[0165]

[0166] Referring to Figure 4, as a result of EE treatment, the proliferation rate of Raw 264.7 cells was the highest at a concentration of 500 μg / ml (57.05%, 1330 cells) compared to the control group (18.82%, 665.5 cells). However, at a concentration of 1000 μg / ml, the cell proliferation rate was significantly reduced (7.8%, 348.3 cells), indicating that EE at a concentration of 1000 μg / ml can be toxic to cells.

[0167]

[0168] When EE and LPS were treated together, it was observed that the cell proliferation rate was significantly reduced compared to the group treated with only EE (23.26%, 368.1 cells) along with morphological changes due to LPS. Nevertheless, it was confirmed that the proliferation rate was higher than the control group up to a concentration of 3.91 ㎍ / ml (31.03%, 387.4 cells) in area occupancy ratio and up to a concentration of 15.63 ㎍ / ml (27.68%, 444.1 cells) in cell number.

[0169]

[0170] 3. Evaluation of the efficacy of raw material extracts on LPS-mediated inflammatory responses in macrophages.

[0171] (1) Evaluation of the effects on inflammatory cytokines and nitric oxide synthase levels at the mRNA level

[0172]

[0173] Figure 5 is a graph showing the evaluation of DE anti-inflammatory efficacy at the mRNA level in LPS-induced inflammatory response.

[0174]

[0175] The anti-inflammatory effect of the water extract (DE) of the herbal medicine powder according to one embodiment of the technical idea of ​​the present invention was observed at the mRNA level in an LPS-induced inflammatory response.

[0176] We measured how the mRNA expression levels of inflammatory cytokines (TNF, IL6, IL1b) and nitric oxide synthase (NOS2) changed in response to various concentrations of DE (0 μg / ml to 100 μg / ml) in the control (CTL) and LPS-treated groups.

[0177]

[0178] DE treatment showed a tendency to significantly suppress the LPS-induced inflammatory response. In particular, in cells treated with 10 ㎍ / ml of DE, the mRNA expression levels of TNF, Il6, Il1b, and NOS2 were reduced by 41%, 36%, 33%, and 36%, respectively, compared to the control group, and all of the results were significant in the T-test.

[0179] This indicates that DE can effectively suppress the production of these inflammatory cytokines. A concentration-dependent decrease was observed from 0 μg / ml to 10 μg / ml, but this reduction effect was observed to diminish at concentrations above 50 μg / ml.

[0180]

[0181] These results suggest that DE may be effective in suppressing LPS-induced inflammatory responses in macrophages, with significant anti-inflammatory effects even at low concentrations.

[0182] Therefore, DE is suggested to have value as a potential raw material for the development of therapeutic agents for inflammatory diseases.

[0183]

[0184] Figure 6 is a graph showing the evaluation of EE anti-inflammatory efficacy at the mRNA level in LPS-induced inflammatory response.

[0185]

[0186] The anti-inflammatory effect of an ethanol extract (EE) of a herbal medicine powder according to one embodiment of the technical idea of ​​the present invention was observed at the mRNA level in an LPS-induced inflammatory response.

[0187] We measured how the mRNA expression levels of inflammatory cytokines (TNF, IL6, IL1b) and nitric oxide synthase (NOS2) changed in response to various concentrations of EE (0 μg / ml to 100 μg / ml) in the control (CTL) and LPS-treated groups.

[0188]

[0189] EE treatment tended to significantly suppress the LPS-induced inflammatory response. In particular, in cells treated with 1 μg / ml of EE, the mRNA expression levels of Il6, Il1b, and NOS2 were reduced by 21%, 21%, and 18%, respectively, compared to the control group, and all results were significant in the T-test.

[0190] This indicates that EE can effectively suppress the production of these inflammatory cytokines. A concentration-dependent decrease was observed from 0 μg / ml to 1 μg / ml, but this reduction effect was observed to diminish at concentrations above 5 μg / ml.

[0191]

[0192] However, TNF mRNA expression levels increased across all sections compared to the control group, indicating that substances contained in EE may selectively act on specific inflammatory pathways, suggesting that the composition of the water extract (DE) and ethanol extract (EE) may be different.

[0193]

[0194] Consequently, EE may be effective in suppressing LPS-induced inflammatory responses in macrophages, and exhibits significant anti-inflammatory effects, particularly at low concentrations (1 μg / ml).

[0195] Therefore, DE is suggested to have value as a potential raw material for the development of therapeutic agents for inflammatory diseases.

[0196]

[0197] (2) Analysis of the effect of regulating nitric oxide production of herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention

[0198]

[0199] FIG. 7 is a graph showing the inhibitory effect of a herbal medicine powder extract on nitric oxide production in RAW 264.7 cells according to one embodiment of the technical idea of ​​the present invention.

[0200]

[0201] Nitric oxide (NO) is a highly reactive, low-molecular-weight inorganic compound produced by the conversion of L-arginine to L-citrulline by the enzyme nitric oxide synthase (NOS). NO is involved in central physiological processes such as vasodilation, nerve signaling, and blood coagulation, and plays an essential role in various endogenous mechanisms, including promoting apoptosis, the suicide program of cancer cells.

[0202] However, when exposed to various inflammatory factors (PS, interferon-γ (IFN-γ), IL-1, TNF-α), excessive production of NO can cause excessive dilation of blood vessels, promotion of inflammatory responses to tissues, occurrence of genetic mutations, and damage to the nervous system, which can lead to various pathological conditions that fall under the category of immune system diseases.

[0203] In this experiment, in order to investigate the effect of a herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention on NO production, the amount of NO production was measured at 450 nm using Griess reagent in RAW 264.7 cells induced with an inflammatory response by LPS.

[0204]

[0205] Referring to Figure 7, the amount of NO production in RAW 264.7 cells that were stimulated with inflammation by LPS was measured, and it was observed that the amount of NO produced by LPS treatment was measured to be 28.66 μM, which is approximately 9 times higher than 3.04 μM in the untreated control group.

[0206]

[0207] When the herbal medicine powder extracts (DE and EE) according to one embodiment of the technical idea of ​​the present invention were treated at concentrations that do not show toxicity to cells (0.1, 0.5, 1, 5, 10, 50, 100 μg / ml), it was confirmed that the production of NO was significantly reduced compared to the LPS treatment group, and in particular, the DE group showed a 30% inhibitory effect even at a relatively low concentration of 10 μg / ml, and the EE group showed a 10% inhibitory effect at 1 μg / ml.

[0208] The results of this experiment suggest that the water extract (DE) and ethanol extract (EE) of the herbal medicine powder according to one embodiment of the technical idea of ​​the present invention can suppress the production of NO.

[0209]

[0210] (3) Study on the regulation of inflammatory cytokine secretion by herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention.

[0211]

[0212] Figure 8 is a graph showing the inhibition of LPS-induced inflammatory cytokine secretion by a herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention.

[0213]

[0214] IL-6 is a key cytokine that mediates inflammation in the immune system. It is produced by several immune cell types, including T cells, monocytes, macrophages, and synovial fibroblasts, and is released by mast cells to enhance antibody production by B lymphocytes and promote the development of T lymphocytes.

[0215] IL-1β is essential for cell growth and maintenance of body balance at low concentrations, but when inflammation is initiated, it regulates the immune response, and when produced in excess by continuous stimulation, it promotes T cell activation and B cell growth, worsening symptoms.

[0216] Therefore, factors that can effectively regulate such inflammatory cytokines may have potential value in contributing to the treatment of various diseases caused by inflammation.

[0217]

[0218] In this experiment, in order to confirm the inhibitory effect of the herbal medicine powder extracts (DE and EE) according to one embodiment of the technical idea of ​​the present invention on the secretion of inflammatory cytokines, in an experiment using RAW 264.7 cells, the secretion of cytokines was promoted through treatment with LPS, known as an inflammatory inducer, and then the herbal medicine powder extracts according to one embodiment of the technical idea of ​​the present invention were added at various concentrations to observe the effect on the secretion of cytokines.

[0219] According to the results measured through ELISA experiments, the herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention significantly reduced the secretion of IL-6 and IL-1β in particular.

[0220] Specifically, referring to Fig. 8(A), DE showed an IL-6 reduction effect of about 25% at 10 μg / ml, and EE showed a reduction effect of 13% at 1 μg / ml.

[0221] Referring to Fig. 8(B), a similar trend was observed for IL-1β, with DE showing a reduction effect of 3% at 10 μg / ml and EE showing a reduction effect of 17% at 1 μg / ml.

[0222] These results provide promising evidence that the herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention may be useful in the treatment and management of diseases associated with inflammatory responses.

[0223]

[0224] 4. Identification of the anti-inflammatory mechanism of raw material extracts in an LPS-induced inflammatory cell model.

[0225] Analysis of the expression level of inflammatory activity biomarkers by herbal medicine powder extracts according to one embodiment of the technical idea of ​​the present invention

[0226]

[0227] Figure 9 is a graph showing the anti-inflammatory control mechanism of a herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention in an LPS-induced inflammatory response.

[0228]

[0229] LPS and cytokines, which induce inflammation, promote the NF-κB signaling pathway, inducing the expression of enzymes such as iNOS. The resulting large amounts of NO have detrimental effects on cells, exacerbating the inflammatory response along with increased vascular permeability. NF-κB is a key factor in regulating the expression of inflammation-related genes, influencing the expression of inflammatory mediators, including iNOS.

[0230]

[0231] In this experiment, prior to exploring the anti-inflammatory mechanism of the herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention, a concentration that is non-cytotoxic and has excellent anti-inflammatory effects was selected with reference to the results of previous studies.

[0232] In an initial experiment, it was observed that the herbal medicine powder water extract (DE) according to one embodiment of the technical idea of ​​the present invention did not exhibit cytotoxicity at 10 μg / ml, and the ethanol extract (EE) at 1 μg / ml, and inhibited the mRNA expression, production, and secretion of inflammatory biomarkers induced by LPS.

[0233]

[0234] 5. Conclusion

[0235] This experiment was conducted to verify the anti-inflammatory effect of herbal medicine powder extracts (DE and EE) according to one embodiment of the technical idea of ​​the present invention, and in this process, the secretion of inflammatory cytokines and protein expression of iNOS, IkB, and NF-κB pathways were measured.

[0236]

[0237] Through a cell proliferation assay using a real-time cell culture analysis (Incucyte), it was shown that the herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention does not cause toxicity to cell survival, but rather increases cell proliferation up to a certain concentration and can resist apoptosis induced by LPS.

[0238]

[0239] As a result of additional experiments to evaluate the anti-inflammatory effect of the extracts, the herbal medicine powder extract according to an embodiment of the technical idea of ​​the present invention reduced the secretion of NO and IL-6 and IL-1β, and in particular, the herbal medicine powder water extract (DE) according to an embodiment of the technical idea of ​​the present invention showed inhibitory effects of 30%, 25%, and 43% at a concentration of 10 ㎍ / ml, respectively, and the Ubimu-hwan ethanol extract (EE) showed inhibitory effects of 10%, 13%, and 17% at a concentration of 1 ㎍ / ml, respectively. In a subsequent experiment, it was confirmed that the Ubimu-hwan extract effectively inhibited the protein expression of the iNOS and NF-κB pathways increased by LPS.

[0240]

[0241] In summary, the herbal medicine powder extract according to one embodiment of the technical idea of ​​the present invention has the potential to effectively respond to inflammatory reactions by suppressing the mRNA expression of NOS2, TNF, Il6, and Il1b through regulation of NF-κB, thereby reducing the secretion of NO, IL-6, and IL-1β, and thus has high applicability as an anti-inflammatory agent.

[0242]

[0243] While a preferred embodiment of the present invention has been described above with reference to the attached drawings, those skilled in the art will appreciate that the present invention can be implemented in other specific forms without altering the technical concept or essential features thereof. Therefore, the above-described embodiment should be understood to be illustrative in all respects and not restrictive.

Claims

1. Preparation of herbal medicine ingredients (S100) A herbal medicine drying step (S200) of drying the prepared herbal medicine to remove moisture; A herbal medicine material grinding step (S300) for grinding the dried herbal medicine material to produce herbal medicine material powder; A herbal medicine mixing step (S400) for producing a herbal medicine mixed powder by mixing the herbal medicine powder and fructooligosaccharide at a certain weight ratio; and A method for manufacturing an herbal medicine with excellent anti-inflammatory effects, characterized by including a pill manufacturing step (S500) of manufacturing an herbal medicine by forming the above herbal medicine ingredient mixture powder into a pill.

2. In paragraph 1, A method for manufacturing a herbal medicine with excellent anti-inflammatory effects, characterized in that in the herbal medicine ingredient preparation step (S100), the herbal medicine ingredients include indong, platycodon grandiflorum, burdock, Ophiopogon japonicus, wolfberry, ginseng, buckwheat, coix seed, magnolia berry, ginger, peppermint and green tea.

3. In paragraph 2, A method for manufacturing an oriental medicine preparation having excellent anti-inflammatory effects, characterized in that in the above herbal ingredient mixing step (S400), the herbal ingredient mixed powder contains 18 to 22 parts by weight of indigo powder, 12 to 17 parts by weight of platycodon root powder, 8 to 12 parts by weight of burdock powder, 3 to 7 parts by weight of Ophiopogon japonicus powder, 3 to 7 parts by weight of wolfberry powder, 3 to 7 parts by weight of ginseng powder, 3 to 7 parts by weight of buckwheat powder, 3 to 7 parts by weight of coix seed powder, 3 to 7 parts by weight of magnolia berry powder, 3 to 7 parts by weight of dried ginger powder, 3 to 7 parts by weight of peppermint powder, 3 to 7 parts by weight of green tea powder, and 3 to 7 parts by weight of fructooligosaccharide.

4. An herbal medicine with excellent anti-inflammatory effect, characterized by being manufactured by any one of the methods selected from clauses 1 to 3.

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