A chinese medicine preparation of phellodendri chinensis cortex for inhibiting tomato stem base rot and its using method

Abstract

The application discloses a Chinese herbal medicine cortex phellodendri preparation for inhibiting tomato stem base rot and a use method thereof, and the Chinese herbal medicine preparation is the cortex phellodendri preparation, and the preparation method is as follows: a proper amount of cortex phellodendri is taken into a glass container, 10 times the volume of water of the Chinese herbal medicine is added, and soaking is carried out for 1 hour; heating is carried out for 30 minutes, medicinal liquid is collected through filtration, an equal amount of water is added, and heating is carried out for 30 minutes; medicinal liquid is collected through filtration, and the two times of medicinal liquid are combined and diluted to a required concentration; and the use method is as follows: after the tomato plants are diseased, the tomato seedlings are treated in a root irrigation mode, the treatment is carried out once every three days, and the treatment is carried out for three times. The cortex phellodendri preparation is a new type of pollution-free biological stimulant, improves the resistance of the tomato to the stem base rot, can significantly reduce the symptoms of the tomato stem base rot, promotes the growth of the plants, enhances the photosynthetic capacity of the leaves and improves the antioxidant enzyme activity. Compared with traditional chemical pesticides, the preparation has obvious treatment effect and is environment-friendly, and the product is safer.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant biostimulants, in particular to a Chinese herbal medicine cortex phellodendri preparation for inhibiting tomato stem base rot and a use method thereof. BACKGROUND

[0002] Tomato is one of the widely planted solanaceous vegetables in the world, which is deeply loved by consumers due to its good taste, various varieties and rich nutrition. Stem base rot caused by Sclerotinia sclerotiorum is one of the soil-borne diseases that easily occur in the growth of northern facility tomatoes in recent years, which has caused great losses to the tomato industry.

[0003] At present, chemical control is the most commonly used means to prevent and control tomato stem base rot, however, long-term use of chemical pesticides will cause a series of environmental problems. Biostimulants have excellent weight loss and efficiency enhancement, resistance improvement, etc. and are widely used in agricultural production. Biostimulants mainly include humic acid, protein hydrolysate and other nitrogen-containing compounds, seaweed extract, chitosan and other biopolymers, inorganic compounds, microbial inoculants, etc. When they are applied to plant leaves or rhizosphere, they can stimulate physiological responses of plants, thereby promoting the absorption and utilization of nutrients by plants, improving the ecological environment of crop rhizosphere, and improving the ability of crops to resist biological stress. In recent years, biostimulants have attracted more and more attention and selection of fertilizer enterprises and consumers due to their fertilizer efficiency and soil improvement effects.

[0004] China has rich resources of Chinese herbal medicine. Natural Chinese herbal medicine has the characteristics of high efficiency, safety, no residue, etc. which ordinary chemical agents do not have, and can solve the problems of pathogenic bacteria resistance, environmental pollution and pesticide residues. With the development of green agriculture, Chinese herbal medicine is more and more widely used in biological control. Cortex phellodendri, as a traditional Chinese herbal medicine in China, belongs to Rutaceae, and its dried bark is used as medicine. It has a wide range of pharmacological activities, including anti-inflammatory, antifungal, antioxidant and other pharmacological effects. It is cheap and resourceful, and is a good resource for developing new biostimulants. Therefore, by selecting a Chinese herbal medicine with a moderate price, an environment-friendly Chinese herbal medicine extract is prepared, which is applied in the field of crop disease resistance, and a new way for the research and development of natural green and pollution-free biostimulants is opened up.

[0005] Therefore, the present application provides a Chinese herbal medicine cortex phellodendri preparation for inhibiting tomato stem base rot and a use method thereof. SUMMARY

[0006] The present application aims to provide a Chinese herbal medicine cortex phellodendri preparation for inhibiting tomato stem base rot and a use method thereof.

[0007] To achieve the above-mentioned purpose, the present application adopts the following technical solutions:

[0008] The first aspect of the present application provides a Chinese herbal medicine preparation for inhibiting tomato stem base rot, wherein the Chinese herbal medicine preparation comprises a component of Huangbai preparation.

[0009] The second aspect of the present application further provides a preparation method of the Chinese herbal medicine preparation for inhibiting tomato stem base rot, comprising the following steps:

[0010] (1) A certain amount of Huangbai is weighed and put into a glass container, and 10 times the volume of water is added to soak for 1 hour;

[0011] (2) The glass container is placed in a 100℃ water bath for 30 minutes, and the filtrate is collected by using a funnel and filter paper; then the same amount of water is added to the residue, and the mixture is heated in a 100℃ water bath for 30 minutes;

[0012] (3) The filtrate is collected by using a funnel and filter paper, and the two filtrates are combined and diluted to the required concentration, which is 4, 8 or 12 mg / mL.

[0013] Preferably, the water used in steps 1 and 2 is distilled water.

[0014] The third aspect of the present application further provides a method for inducing tomato stem base rot, comprising the following steps:

[0015] (1) In the three-leaf one-heart stage of tomato, a sterile cotton swab is used to pick up the Fusarium solani liquid and scratch the stem base;

[0016] (2) A puncher is used to punch the PDA medium with the Fusarium solani;

[0017] (3) The mycelium block is placed opposite to the scratch position, and the cotton swab is used to pick up the Fusarium solani liquid to wrap the position for inducing the disease;

[0018] (4) A transparent cover is placed on the tray, water is sprayed on the cover to provide a humid condition for the disease, and the tray is placed in a weak light for cultivation.

[0019] The fourth aspect of the present application further provides a use method of the Chinese herbal medicine preparation for inhibiting tomato stem base rot, wherein the crop is tomato, and the action site is the stem base; after the tomato plant is infected, the tomato seedlings are treated by root irrigation, and the treatment is performed once every three days for a total of three times.

[0020] The present application provides a Chinese herbal medicine Huangbai preparation for inhibiting tomato stem base rot and a use method thereof, and the beneficial effects are as follows:

[0021] 1. This invention uses a natural Chinese herbal medicine, Phellodendron bark, as a biostimulant to treat tomato plants through root irrigation. This significantly reduces the symptoms of tomato stem rot, promotes plant growth, enhances leaf photosynthetic capacity, and increases antioxidant enzyme activity. Therefore, Phellodendron bark preparation, as a new type of natural, green, and pollution-free biostimulant, can not only improve the resistance of tomatoes to stem rot, but also does not cause heavy metal and pesticide residues in tomato plants, thus having higher safety.

[0022] 2. The formula of this invention is a pure Chinese medicine formula, which is a natural substance with little toxicity and side effects, mild efficacy, and no drug resistance. It can be effectively absorbed by organisms and does not easily cause soil pollution. It is safe, pollution-free, residue-free, green, healthy and environmentally friendly, and can effectively improve the benefits of tomato planting. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This invention provides a schematic diagram illustrating the effects of different concentrations of Phellodendron amurense preparations on the aboveground phenotype of tomato plants after inoculation with stem rot. Figure A shows the overall plant growth phenotype; Figure B shows the lesion at the stem base; Figure C shows the area of ​​the lesion at the stem base; Figure D shows the plant height; Figure E shows the stem diameter; and sterile water indicates that the plant was not infected with stem rot and was treated with sterile water.

[0025] Figure 2 This is a schematic diagram illustrating the effect of different concentrations of Phellodendron bark preparations provided by the present invention on the size of tomato roots after grafting with disease; wherein, Figure A is the tomato root phenotype; Figure B is the total root length; C is the root surface area; D is the root volume; E is the average root diameter; F is the number of root tips; G is the fractal dimension; sterile water indicates that the plant has no grafting stem rot and has been treated with sterile water;

[0026] Figure 3 This is a schematic diagram illustrating the effect of different concentrations of Phellodendron bark preparations provided by the present invention on the biomass of tomato plants after grafting with disease; wherein, Figure A is the fresh weight of the aboveground part of the plant; Figure B is the fresh weight of the underground part; Figure C is the dry weight of the aboveground part; Figure D is the dry weight of the underground part; Figure E is the fresh root-to-shoot ratio; Figure F is the dry root-to-shoot ratio; sterile water indicates that the plant has no grafting stem rot and has been treated with sterile water;

[0027] Figure 4This diagram illustrates the effects of different concentrations of Phellodendron amurense preparations provided by this invention on the photosynthetic parameters of tomato leaves; where Figure A represents the net photosynthetic rate; Figure B represents the intercellular carbon dioxide concentration; Figure C represents the stomatal conductance; Figure D represents the transpiration rate; Figure E represents the actual photochemical efficiency of photosystem II; Figure F represents the maximum photochemical efficiency of photosystem II; sterile water indicates that the plant is free of graft rot.

[0028] Figure 5 This diagram illustrates the effect of different concentrations of Phellodendron bark extract on photosynthetic pigments in tomato leaves after grafting disease. Figure A shows the chlorophyll a content of tomato leaves; Figure B shows the chlorophyll b content; Figure C shows the total chlorophyll content; Figure D shows the carotenoid content; and sterile water indicates that the plant was free of graft basal rot and treated with sterile water.

[0029] Figure 6 The diagram illustrates the effects of different concentrations of the Phellodendron amurense preparation provided by this invention on the antioxidant enzyme activity and malondialdehyde (MDA) content of tomato leaves after grafting disease. Figure A shows the superoxide dismutase (SOD) activity in the leaves; Figure B shows the peroxidase (PERO) activity; Figure C shows the catalase (CAT) activity; Figure D shows the MDA content; sterile water indicates that the plants were free of grafting stem rot and were treated with sterile water. Detailed Implementation

[0030] To make the technical means, creative features, and achieved objectives and effects of this invention easier to understand, the invention is further described below in conjunction with specific embodiments. Obviously, the described embodiments are only a part of the embodiments of this invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0031] Example:

[0032] like Figures 1 to 6 As shown, the present invention provides a traditional Chinese medicine preparation of Phellodendron bark that inhibits stem rot in tomatoes, wherein the component of the traditional Chinese medicine preparation is Phellodendron bark.

[0033] In this embodiment, the preparation of the traditional Chinese medicine Phellodendron bark was obtained through the following steps:

[0034] (1) Weigh an appropriate amount of Phellodendron bark and put it into a glass container. Add water with a volume of 10 times that of the Chinese herbal medicine and soak for 1 hour.

[0035] (2) Heat in a 100°C water bath for 30 minutes, filter the liquid using a funnel and filter paper, add an equal amount of water to the residue, and heat in a 100°C water bath for 30 minutes.

[0036] (3) Collect the drug solution by filtering with a funnel and filter paper, combine the two drug solutions, and dilute to the required concentrations of 4, 8 and 12 mg / mL.

[0037] The method of inoculating tomato stem base rot in this embodiment is as follows:

[0038] (1) At the three-leaf one-heart stage of tomato, use a sterilized cotton swab to pick up the S. lycopersicola bacteria solution and scratch the stem base;

[0039] (2) Use a puncher to punch the PDA medium with S. lycopersicola;

[0040] (3) Place the mycelium block opposite the scratch position, and use sterilized cotton to wrap the inoculated position with the S. lycopersicola bacteria solution;

[0041] (4) Cover a transparent cover on the tray, spray water on the cover to provide humid conditions for disease, and place it in a weak light place for culture.

[0042] The use method of the Chinese herbal medicine preparation in this embodiment is as follows:

[0043] After the tomato plants are diseased, the tomato seedlings are treated by root irrigation, once every three days, for a total of three times.

[0044] Among them, the acting crop is tomato, and the acting position is the stem base.

[0045] Test example: The tested tomato variety is “Heinz 1706”.

[0046] Test grouping: The test material of this study is the tomato cultivar “Heinz 1706”. The seeds are first washed with 75% anhydrous ethanol on a clean bench for 1 minute, then soaked with 25% sodium hypochlorite, shaken at 28°C for 20 minutes, and then washed with sterile water for 10 times. After the seed coat is slightly whitened, it is sown in MS medium;

[0047] After 3 days of dark culture in a 28°C incubator, it is transferred to light culture. After the first true leaf of the tomato seedling grows, it is transplanted into a mixed substrate of vermiculite, perlite and soil in a ratio of 1:1:2, and watered thoroughly. It is grown under the conditions of a light cycle of 16 hours (light) and 8 hours (dark), a day and night temperature cycle of 25°C / 20°C, a light intensity of 150 μmol·m -2 ·s -1 and a relative humidity of 50%;

[0048] After the stem base is diseased, the cotton is removed, and the stem base is treated with 0, 4, 8, and 12 mg / mL concentrations of Chinese herbal medicine preparation by root irrigation. Sterile water indicates that the plant is not infected with stem base rot bacteria and is treated with sterile water. Each tomato seedling is irrigated with 30 ml of Chinese herbal medicine preparation, and the treatment is performed once every 3 days for a total of 3 times.

[0049] Test method: complete randomized block design, 6 biological replicates are set.

[0050] Experimental results:

[0051] Refer to the Figure 1 The disease spot area of the tomato treated with different concentrations of the preparation of Cortex Phellodendri showed significant differences at the statistical level, and the trend of first decreasing and then increasing was observed with the increase of the treatment concentration. The inhibition effect on the disease spot area was the most obvious at the treatment concentration of 8 mg / mL. The plant height of the tomato treated with 8 mg / mL was increased by 40.44% and the stem diameter was increased by 11.27% compared with the control group;

[0052] Refer to the Figure 2 The root length, root surface area, root volume, root average diameter and root tip number of the tomato treated with different concentrations of the preparation of Cortex Phellodendri were significantly increased, and the trend of first increasing and then decreasing was observed with the increase of the treatment concentration, and the peak value was observed at the treatment concentration of 8 mg / mL;

[0053] Refer to the Figure 3 Compared with the control group, only the aboveground and underground dry weights were significantly increased at the treatment concentration of 8 mg / mL, and the promotion effect of the rest treatments was not significant. There was no significant difference in the fresh root crown ratio among the treatments, but the dry root crown ratio of the treatment at 8 mg / mL was significantly increased by 22.60% compared with the treatment at 0 mg / mL. The fresh root crown ratio was the largest at the treatment concentration of 4 mg / mL, which was significantly increased by 12.89% compared with the treatment at 0 mg / mL;

[0054] Refer to the Figure 4 The stomatal conductance and transpiration rate of the tomato treated with 8 mg / mL were also significantly increased, which were increased by 67.95% and 39.50% compared with the treatment at 0 mg / mL, respectively, and the intercellular carbon dioxide concentration was decreased by 13.55% at the treatment of 8 mg / mL. The ΦPSII (the actual photochemical efficiency of photosystem II) and Fv / Fm (the maximum photochemical efficiency of photosystem II) were significantly increased at the treatment concentrations of 4 mg / mL and 8 mg / mL compared with the two control groups, and the change was not significant at the treatment concentration of 12 mg / mL;

[0055] Refer to the Figure 5 The contents of chlorophyll a and chlorophyll b were 0.4458 mg / g and 0.1270 mg / g at the treatment concentration of 4 mg / mL, which were increased by 44.18% and 37.74% compared with the treatment at 0 mg / mL, respectively. There was no significant difference in the content of carotenoid, but the maximum value was reached at the treatment concentration of 4 mg / mL, which was increased by 14.43% compared with the treatment at 0 mg / mL;

[0056] Refer to the Figure 6Compared with the non-disease group, the antioxidant enzyme activity of the disease tomato leaves treated by the cortex phellodendri preparation was significantly higher than that of the non-treated group after the disease. The SOD (superoxide dismutase activity), POD (peroxidase activity), and CAT (catalase activity) activities of the tomato leaves after the disease showed a trend of first increasing and then decreasing under different concentrations of treatment, and reached the maximum at 8 mg / mL. The MDA (malondialdehyde content) content of the tomato leaves after the disease was significantly higher than that of the non-disease control group, and showed a trend of first decreasing and then increasing among different concentrations of treatment, and was the lowest at the 8 mg / mL treatment concentration, which was 67% lower than that of the 0 mg / mL treatment.

[0057] The above shows and describes the basic principles and main features of the present application and the advantages of the present application. Those skilled in the art should understand that the present application is not limited to the above-mentioned embodiments, and the above-mentioned embodiments and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. Use of a Chinese herbal preparation for promoting the growth of tomato plants, characterized in that, The Chinese herbal medicine preparation is a preparation of Phellodendri Chinensis Cortex; the preparation method of the Phellodendri Chinensis Cortex preparation comprises the following steps: (1) a proper amount of Phellodendri Chinensis Cortex is weighed and put into a glass container, 10 times the volume of water of the Chinese herbal medicine is added, and soaked for 1 hour; (2) heated in a 100 DEG C water bath for 30 minutes, filtered with a funnel and filter paper to collect the medicinal liquid, and then the same amount of water is added to the residue, heated in a 100 DEG C water bath for 30 minutes; (3) filtered with a funnel and filter paper to collect the medicinal liquid, and the medicinal liquids of the two times are combined, diluted to the required concentration, and the concentration is 8 mg / mL.

2. Use of the Chinese herbal preparation according to claim 1 for promoting the growth of tomato plants, characterized in that, The water used in the steps (1) and (2) is distilled water.

3. Use of the Chinese herbal preparation according to claim 1 for promoting the growth of tomato plants, characterized in that, The method comprises the following steps: (1) at the three-leaf-one-heart stage of the tomato, the tomato seedlings are subjected to the inoculation treatment of the stem base rot; (2) after the tomato plants are infected, the Chinese herbal medicine Phellodendri Chinensis Cortex preparation is used to treat the tomato seedlings, once every three days, and the treatment is performed for three times; The inoculation treatment of the stem base rot of the tomato seedlings comprises the following steps: (1) at the three-leaf-one-heart stage of the tomato, a sterilized cotton swab is used to pick up the Fusarium solani liquid, and the stem base is scratched; (2) a puncher is used to punch the PDA culture medium with the Fusarium solani; (3) the mycelium block is directly opposite to the scratched position, and the cotton swab is used to pick up the Fusarium solani liquid to wrap the inoculation position; (4) a transparent cover is covered on the tray, water is sprayed on the cover to provide humid conditions for the disease, and the tray is placed in a weak light place for culture, and the tomato plants are waited to be infected.

4. Use of the Chinese herbal preparation according to claim 3 for promoting the growth of tomato plants, characterized in that, The treatment part of the tomato seedlings is the stem base, and the treatment mode is root irrigation.

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