Low-intensity intermittent stress model and apparatus

Abstract

The present invention relates to the field of model testing, in particular to a low-intensity intermittent stress model and an apparatus. The model and the apparatus comprise: observing a target body within a set time, wherein the set time comprises five time period groups, and each time period group comprises three days. The responses of the target body to corresponding stresses are obtained by means of the stress observation of each group of three days.

Description

A model and device for low-intensity intermittent stress Technical Field

[0001] The present invention relates to the field of model testing, and in particular to a low-intensity intermittent stress model and device. Background Art

[0002] Currently, there are hundreds of millions of depression patients worldwide. Not only do these patients have a very poor life experience, but they also suffer from a huge socioeconomic burden. As we all know, stress has two sides. Long-term severe negative stress can cause or trigger mental illnesses such as depression. However, low-intensity intermittent stress is beneficial for sharpening the mind and promoting the building of self-confidence, thereby overcoming difficulties and getting out of the haze. Consistent with the principle of extinction therapy for post-traumatic stress disorder, the use of low-intensity intermittent stress (LIIS) in the treatment of depression and its mechanism are still unclear. Therefore, a mouse model that can experimentally test low-intensity intermittent stress is needed.

[0003] Summary of the Invention

[0004] The embodiment of the present invention provides a model and device for low-intensity intermittent stress, so as to provide a model of a target body capable of low-intensity intermittent stress.

[0005] According to one embodiment of the present invention, a low-intensity intermittent stress model is provided, comprising:

[0006] Observe the target object within a set time, wherein the set time includes five time periods, each time period including three days;

[0007] On the first day, the target subject was observed for social stress and his / her behavior was recorded;

[0008] On the second day, the target was subjected to restraint stress observation and its behavior was recorded;

[0009] On the third day, no intervention was performed on the target, and the target's behavior was recorded;

[0010] Repeat the above operation for three days as a group until the five time periods of the target object are recorded.

[0011] In one embodiment, social pressure is observed as:

[0012] Record the first target and the second target in the same environment;

[0013] Observe whether a target object in the same environment attacks another target object. If a first target object attacks a second target object within a preset time, the first target object is marked as aggressive.

[0014] In one embodiment, the subject is administered a sugar water preference test.

[0015] In one embodiment, the sugar water preference test comprises:

[0016] Four groups of test subjects were set up to test the sugar water preference, including the olfactory bulbectomy group, the sham operation group, the olfactory bulbectomy plus low-intensity intermittent stress group, and the sham operation plus low-intensity intermittent stress group.

[0017] A low-intensity intermittent stress device, comprising:

[0018] The social pressure module is used to observe the social pressure of the target on the first day and record the target's behavior;

[0019] The restraint stress module is used to observe the restraint stress of the target on the second day and record the target's behavior;

[0020] The non-intervention recording module is used to record the target's behavior without any intervention on the third day;

[0021] Repeat the above operation for three days as a group until the five time periods of the target object are recorded.

[0022] In one embodiment, the social pressure module includes:

[0023] A first recording unit is used to record the first target and the second target in the same environment;

[0024] The second recording unit is used to observe whether a target object attacks another target object in the same environment. If a first target object attacks a second target object within a preset time, the first target object is marked as aggressive.

[0025] In one embodiment, the apparatus further comprises:

[0026] The sugar water test module is used to test the target's preference for sugar water.

[0027] In one embodiment, the sugar water testing module comprises:

[0028] The group setting unit is used to set four groups of test sugar water preference targets, including an olfactory bulb resection group, a sham operation group, an olfactory bulb resection plus low-intensity intermittent stress group, and a sham operation plus low-intensity intermittent stress group.

[0029] A computer-readable storage medium stores one or more programs, and the one or more programs can be executed by one or more processors to implement the steps in any one of the above-mentioned low-intensity intermittent stress models.

[0030] A terminal device comprises: a processor, a memory and a communication bus; the memory stores a computer-readable program that can be executed by the processor;

[0031] The communication bus realizes the connection and communication between the processor and the memory;

[0032] When the processor executes the computer-readable program, the processor implements the steps in any one of the above-mentioned low-intensity intermittent stress models.

[0033] The low-intensity intermittent stress model and device in the embodiments of the present invention observes a subject over a set time period, wherein the set time period includes five time periods, each consisting of three days. The subject's response to the stress is determined through the three-day stress observations. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] FIG1 is a flow chart of a low-intensity intermittent stress model of the present invention;

[0035] FIG2 is a block diagram of a low-intensity intermittent stress device according to the present invention;

[0036] FIG3 is an analysis diagram of the sugar water preference test of the present invention;

[0037] FIG4 is a diagram of a terminal device according to the present invention. DETAILED DESCRIPTION

[0038] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts should fall within the scope of protection of the present invention.

[0039] It should be noted that the terms "first", "second", etc. in the description and claims of the present invention and the above-mentioned drawings are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that the numbers used in this way can be interchanged where appropriate, so that the embodiments of the present invention described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device that includes a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0040] Example 1

[0041] According to one embodiment of the present invention, a model of low-intensity intermittent stress is provided, as shown in FIG1 , including:

[0042] Observe the target object within a set time, wherein the set time includes five time periods, each time period including three days;

[0043] S100: Observe the target's social stress on the first day and record the target's behavior;

[0044] S200: On the second day, the target is subjected to restraint stress observation and its behavior is recorded;

[0045] S300: On the third day, no intervention is performed on the target, and the target's behavior is recorded;

[0046] Repeat the above operation for three days as a group until the five time periods of the target object are recorded.

[0047] The low-intensity intermittent stress model and device in the embodiment of the present invention observes the target body within a set time, wherein the set time includes five groups of time periods, each group of time periods includes three days. Through each group of three days of stress observation, the target body's response to stress is obtained. See Table 1

[0048] Table 1

[0049] In one embodiment, social pressure is observed as:

[0050] Record the first target and the second target in the same environment;

[0051] Observe whether a target object in the same environment attacks another target object. If a first target object attacks a second target object within a preset time, the first target object is marked as aggressive.

[0052] For example, the target object is set as mice. Under social stress environment, mice marked as CD1 are housed in single cages for 7 days to develop territorial awareness, and C57BL / 6 mice are housed for four to five days to adapt to the environment.

[0053] Screen CD1 mice for aggressiveness by placing C57BL / 6 mice in the cage of CD1 mice for three minutes. If the CD1 mice initiate attacks more than five times with an attack latency of less than one minute, they are considered aggressive. If they are aggressive at least twice in 3 days, they can be used as attacking mice in the model.

[0054] The experimental group of C57BL / 6 mice were placed in a CD1 mouse cage and isolated with a transparent acrylic plate with holes for 24 hours after being challenged for five to ten minutes. New CD1 mice were replaced every day to avoid adaptation.

[0055] In one embodiment, the sugar water preference test comprises:

[0056] Four groups of test subjects were set up to test the sugar water preference, including the olfactory bulbectomy group, the sham operation group, the olfactory bulbectomy plus low-intensity intermittent stress group, and the sham operation plus low-intensity intermittent stress group.

[0057] Referring to Figure 3 , the mice in the olfactory bulbectomy group showed a lower percentage of sugar water preference compared to the sham operation group, and the mice in the olfactory bulbectomy plus low-intensity intermittent stress group showed a higher percentage of sugar water preference compared to the mice in the simple olfactory bulbectomy group.

[0058] The model of this application can also be used to conduct experiments on low-intensity intermittent stress in humans: specific stress tasks can be formulated according to the characteristics of specific patients, and such tasks are conducive to improving the patient's own value and ability. The total paradigm time is 30 days, with 2 days of each stress and 1 day of interval, for a total of 10 cycles.

[0059] This application constructs a low-intensity intermittent stress model from the perspective of adaptability to stress to reduce the sensitivity of depression to stress, thereby being used for the treatment of depression; this model paradigm is low-cost, simple, and highly operational, which is conducive to the promotion of future clinical trials.

[0060] Example 2

[0061] According to another embodiment of the present invention, a low-intensity intermittent stress device is provided, as shown in FIG2 , comprising:

[0062] Observe the target object within a set time, wherein the set time includes five time periods, each time period including three days;

[0063] The social pressure module 100 is used to observe the social pressure of the target on the first day and record the target's behavior;

[0064] The restraint stress module 200 is used to observe the restraint stress of the target on the second day and record the target's behavior;

[0065] The non-intervention recording module 300 is used for not performing any intervention on the target body on the third day and recording the target body's behavior;

[0066] Repeat the above operation for three days as a group until the five time periods of the target object are recorded.

[0067] The low-intensity intermittent stress model and device in the embodiments of the present invention observes a subject over a set time period, wherein the set time period includes five time periods, each consisting of three days. The subject's response to the stress is determined through the three-day stress observations.

[0068] In one embodiment, social pressure is observed as:

[0069] A first recording unit records the first target and the second target in the same environment;

[0070] The second recording unit observes whether a target object attacks another target object in the same environment. If a first target object attacks a second target object within a preset time, the first target object is marked as aggressive.

[0071] In one embodiment, the apparatus further comprises:

[0072] The sugar water test module is used to test the target's preference for sugar water.

[0073] In one embodiment, the sugar water testing module comprises:

[0074] The group setting unit is used to set four groups of test sugar water preference targets, including an olfactory bulb resection group, a sham operation group, an olfactory bulb resection plus low-intensity intermittent stress group, and a sham operation plus low-intensity intermittent stress group.

[0075] Referring to Figure 3 , the mice in the olfactory bulbectomy group showed a lower percentage of sugar water preference compared to the sham operation group, and the mice in the olfactory bulbectomy plus low-intensity intermittent stress group showed a higher percentage of sugar water preference compared to the mice in the simple olfactory bulbectomy group.

[0076] Example 3

[0077] Based on the above-mentioned model of low-intensity intermittent stress, this embodiment provides a computer-readable storage medium, which stores one or more programs. The one or more programs can be executed by one or more processors to implement the steps in the model of low-intensity intermittent stress in the above-mentioned embodiment.

[0078] Example 4

[0079] A terminal device includes: a processor, a memory, and a communication bus; the memory stores a computer-readable program that can be executed by the processor; the communication bus realizes connection and communication between the processor and the memory; when the processor executes the computer-readable program, it realizes the steps in the above-mentioned low-intensity intermittent stress model.

[0080] Based on the above-mentioned model of low-intensity intermittent stress, the present application provides a terminal device, as shown in Figure 4, which includes at least one processor (processor) 20; a display screen 21; and a memory (memory) 22, and may also include a communication interface (Communications Interface) 23 and a bus 24. Among them, the processor 20, the display screen 21, the memory 22 and the communication interface 23 can communicate with each other through the bus 24. The display screen 21 is configured to display a preset user guide interface in the initial setting mode. The communication interface 23 can transmit information. The processor 20 can call the logic instructions in the memory 22 to execute the method in the above embodiment.

[0081] In addition, the logic instructions in the memory 22 can be implemented in the form of software functional units and can be stored in a computer-readable storage medium when sold or used as an independent product.

[0082] The memory 22, as a computer-readable storage medium, can be configured to store software programs or computer-executable programs, such as program instructions or modules corresponding to the methods in the embodiments of the present disclosure. The processor 20 executes the software programs, instructions, or modules stored in the memory 22 to perform functional applications and data processing, thereby implementing the methods in the above embodiments.

[0083] The memory 22 may include a program storage area and a data storage area. The program storage area may store an operating system and at least one application required for a function; the data storage area may store data generated based on the use of the terminal device. Furthermore, the memory 22 may include high-speed random access memory and non-volatile memory. For example, various media that can store program code, such as a USB flash drive, a mobile hard drive, a read-only memory (ROM), a random access memory (RAM), a magnetic disk, or an optical disk, may also be transient storage media.

[0084] In addition, the specific process of loading and executing the multiple instructions in the storage medium and the processor in the terminal device has been described in detail in the above method and will not be described here one by one.

[0085] The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A model of low-intensity intermittent stress, characterized in that, Including: Observing the target body within a set time, where the set time includes five groups of time periods, and each group of the time periods includes three days; On the first day, observing the social stress of the target body and recording the behavior of the target body; On the second day, observing the restraint stress of the target body and recording the behavior of the target body; On the third day, no intervention is performed on the target body, and the behavior of the target body is recorded; Repeat the above operations in groups of three days until the recording of the five groups of time periods for the target body is completed.

2. The model of low-intensity intermittent stress according to claim 1, wherein The social stress observation is: Recording the first target body and the second target body in the same environment; Observing whether the target body attacks another target body in the same environment. If within a preset time, the first target body attacks the second target body, then the first target body is marked as aggressive.

3. The model of low-intensity intermittent stress according to claim 1, wherein Conducting a sucrose preference test on the target body.

4. The model of low-intensity intermittent stress according to claim 3, wherein The sucrose preference test includes: Setting four groups of target bodies for testing sucrose preference, including an olfactory bulbectomy group, a sham operation group, an olfactory bulbectomy plus low-intensity intermittent stress group, and a sham operation plus low-intensity intermittent stress group.

5. A device for low-intensity intermittent stress, characterized in that, Including: A social stress module for observing the social stress of the target body on the first day and recording the behavior of the target body; A restraint stress module for observing the restraint stress of the target body on the second day and recording the behavior of the target body; A no-intervention recording module for not performing any intervention on the target body on the third day and recording the behavior of the target body; Repeat the above operations in groups of three days until the recording of the five groups of time periods for the target body is completed.

6. The device for low-intensity intermittent stress according to claim 1, characterized in that, The social stress module includes: A first recording unit for recording the first target body and the second target body in the same environment; A second recording unit for observing whether the target body attacks another target body in the same environment. If within a preset time, the first target body attacks the second target body, then the first target body is marked as aggressive.

7. The device for low-intensity intermittent stress according to claim 1, characterized in that, The device further includes: A sucrose test module for conducting a sucrose preference test on the target body.

8. The device for low-intensity intermittent stress according to claim 7, characterized in that, The sucrose test module includes: A grouping setting unit for setting four groups of target bodies for testing sucrose preference, including an olfactory bulbectomy group, a sham operation group, an olfactory bulbectomy plus low-intensity intermittent stress group, and a sham operation plus low-intensity intermittent stress group.

9. A computer-readable medium, characterized in that, The computer-readable storage medium stores one or more programs, and the one or more programs can be executed by one or more processors to implement the steps in the low-intensity intermittent stress model as described in any one of claims 1-4.

10. A terminal device, characterized in that, Including: A processor, a memory, and a communication bus; The memory stores a computer-readable program executable by the processor; The communication bus realizes the connection and communication between the processor and the memory; When the processor executes the computer-readable program, it implements the steps in the low-intensity intermittent stress model as described in any one of claims 1-4.

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