Use of skin care active substance in preparing skin care product

Abstract

Provided is use of a skin care active substance in preparing a skin care product. The permeation of the skin care active substance into the skin is promoted by using a nebulizer and / or a permeation-promoting apparatus. The skin care active substance comprises a humectant comprising at least one of ceramide NP, hydrolyzed sodium hyaluronate, sodium hyaluronate, acetylated hyaluronic acid, a sodium hyaluronate cross-linked polymer, and D-panthenol. By promoting the permeation of the skin care active substance with the nebulizer and / or the permeation-promoting apparatus, the present application achieves the moisturizing effect and improves the water content in the stratum corneum of the skin.

Description

Application of skin care active ingredients in the preparation of skin care products

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims priority based on Chinese patent application 202410115298.4 filed on January 25, 2024, and all of its contents are incorporated herein by reference. Technical Field

[0003] The present application relates to the technical field of skin care, and in particular to the application of skin care active ingredients in the preparation of skin care products. Background Art

[0004] Skin is the body's first line of defense against external aggressors, helping to protect the body from harmful substances such as bacteria. Healthy, hydrated skin helps ensure that it constantly renews itself to maintain its protective barrier function.

[0005] Exfoliation is a normal metabolic process of keratinocytes, and our epidermal cells renew themselves every 28 days. With age, this process slows down, sometimes to as little as 40 days. This slowdown can lead to skin problems such as roughness, sallowness, uneven skin tone, hyperpigmentation, fine lines, and wrinkles. Therefore, moisturizing the skin is essential.

[0006] Summary of the Invention

[0007] The present application provides an application of a skin care active substance in the preparation of a skin care product to achieve moisturizing of the skin and increase the water content of the skin's stratum corneum.

[0008] To solve the above technical problems, the first aspect of the present application provides an application of a skin care active in the preparation of a skin care product, using an atomizer and / or a penetration-promoting device to promote the penetration of the skin care active into the skin; wherein, the skin care active includes a moisturizer, and the moisturizer includes at least one of ceramide NP, hydrolyzed sodium hyaluronate, sodium hyaluronate, acetylated hyaluronic acid, sodium hyaluronate cross-linked polymer, and D-panthenol.

[0009] In one embodiment, the skin care active ingredients further include at least one of a moisturizing and firming agent, a soothing and anti-sensitizing agent, an antioxidant, a soothing agent, and an anti-wrinkle and firming agent; the moisturizing and firming agent includes at least one of hydrolyzed lupin protein and XVII collagen; the soothing and anti-sensitizing agent includes peony root extract; the anti-wrinkle and firming agent includes at least one of acetyl hexapeptide-8, tripeptide-1, acetyl octapeptide-3, snake venom peptide, palmitoyl tripeptide-5, palmitoyl tripeptide-1, palmitoyl hexapeptide-12, acetyl tetrapeptide-11, and hexapeptide-3; the antioxidant includes at least one of ergothioneine, coenzyme Q10, VC derivatives, VE derivatives, VC glucoside, tocopheryl acetate, silymarin, and unicorn extract; the soothing agent includes at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol.

[0010] In one embodiment, the skin care active comprises 3-90 parts by weight of the anti-wrinkle and firming agent, 0.1-15 parts by weight of the moisturizer, and 0.05-2.4 parts by weight of other ingredients; wherein the anti-wrinkle and firming agent comprises a first anti-wrinkle and firming agent and / or a second anti-wrinkle and firming agent, the first anti-wrinkle and firming agent comprises at least one of acetyl hexapeptide-8, tripeptide-1, acetyl octapeptide-3, and snake venom peptide; the second anti-wrinkle and firming agent comprises palmitoyl tripeptide-5, palmitoyl tripeptide -1, palmitoyl hexapeptide-12, acetyl tetrapeptide-11, and hexapeptide-3; the moisturizing agent includes at least one of ceramide NP, hydrolyzed sodium hyaluronate, and sodium hyaluronate; the other ingredients include 0.05-0.4 parts by weight of the moisturizing and firming agent and 0.05-2 parts by weight of at least one of the soothing and anti-allergic agents; wherein the moisturizing and firming agent includes at least one of hydrolyzed lupine protein and XVII collagen, and the soothing and anti-allergic agent includes peony root extract.

[0011] In one embodiment, the skin care active is composed of the following components in the following weight ratio: 3-10 parts by weight of acetyl hexapeptide-8, 3-10 parts by weight of palmitoyl tripeptide-5, 0.05-0.2 parts by weight of hydrolyzed lupin protein, 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, and 0.05-2 parts by weight of peony root extract; wherein, in the 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, the weight ratio of the hydrolyzed sodium hyaluronate to the sodium hyaluronate is (2.5-4):(1.5-3).

[0012] In one embodiment, the skin care active ingredients include at least two of 0.0001-12 parts by weight of the antioxidant, 0.05-0.4 parts by weight of the moisturizing and firming agent, 0.05-2 parts by weight of the soothing and anti-sensitizing agent, and 0.1-15 parts by weight of the moisturizing agent, in a weight ratio; wherein the antioxidant includes at least one of ergothioneine, coenzyme Q10, VC derivatives, and VE derivatives; the moisturizing and firming agent includes at least one of hydrolyzed lupin protein and type XVII collagen; the soothing and anti-sensitizing agent includes peony root extract; and the moisturizing agent includes at least one of ceramide NP, hydrolyzed sodium hyaluronate, and sodium hyaluronate.

[0013] In one embodiment, the skin care active is composed of the following components in the following weight ratio: 0.0001-3 parts by weight of ergothioneine, 0.05-0.2 parts by weight of hydrolyzed lupin protein, 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, and 0.05-2 parts by weight of peony root extract; wherein, in the 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, the weight ratio of the hydrolyzed sodium hyaluronate to the sodium hyaluronate is (2.5-4):(1.5-3).

[0014] In one embodiment, the skin care active ingredients include 0.31-13 parts by weight of the antioxidant and 0.1-10 parts by weight of the moisturizer; wherein the antioxidant includes 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, and 0.1-5 parts by weight of unicorn extract; and the moisturizer includes at least one of sodium hyaluronate crosspolymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate.

[0015] In one embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

[0016] In one embodiment, the skin care active comprises 0.1-15 parts by weight of the moisturizing agent and 0.1-12 parts by weight of the soothing agent, wherein the moisturizing agent comprises at least one of quadruple hyaluronic acid and D-panthenol, and the quadruple hyaluronic acid comprises sodium hyaluronate crosspolymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate; and the soothing agent comprises at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol.

[0017] In one embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of quadruple hyaluronic acid, 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of Portulaca oleracea extract, 0.1-1 parts of Piper methysticum extract, and 0.1-5 parts by weight of Kirindra extract; wherein the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate in the quadruple hyaluronic acid is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

[0018] In one embodiment, the skin care active ingredients include 0.1-15 parts by weight of the moisturizer and 0.05-2 parts by weight of the soothing and anti-sensitizing agent, respectively; wherein the moisturizer includes at least two of ceramide NP, hydrolyzed sodium hyaluronate, and sodium hyaluronate; and the soothing and anti-sensitizing agent includes peony root extract.

[0019] In one embodiment, the skin care active is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, 0.05-2 parts by weight of peony root extract, and 0.01-5 parts by weight of acetylglucosamine; wherein, in the 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, the weight ratio of the hydrolyzed sodium hyaluronate to the sodium hyaluronate is (2.5-4):(1.5-3).

[0020] In one embodiment, the permeation promotion device includes at least one electrode group; the control method of the permeation promotion device includes: electrically stimulating the target area based on the electrode groups corresponding to multiple permeation promotion modes; wherein at least some of the working parameters between the permeation promotion modes are different; at least one of the permeation promotion modes corresponds to a first working parameter, and the current output frequency range of the first working parameter is not less than 30kHz and not greater than 50kHz, and the corresponding peak-to-peak value range is 30Vpp to 56Vpp; at least one of the permeation promotion modes corresponds to a second working parameter, and the current output frequency range of the second working parameter is greater than 100kHz and less than 400kHz, and the corresponding peak-to-peak value range is 60Vpp to 70Vpp.

[0021] In one embodiment, the atomizer applies the skin care active ingredient to the skin surface in a spray manner; the atomized particles generated by the spray manner have a D50 particle size of 10 μm to 50 μm.

[0022] In one embodiment, the atomized particles generated by the spraying method have a D50 particle size of 10 μm to 30 μm.

[0023] The beneficial effects of this application are as follows: Different from the prior art, this application discloses the use of a skin care active ingredient in the preparation of a skin care product, using an atomizer and / or a penetration-promoting device to promote the penetration of the skin care active ingredient into the skin. The skin care active ingredient includes a moisturizer, which includes at least one of ceramide NP, hydrolyzed sodium hyaluronate, sodium hyaluronate, acetylated hyaluronic acid, sodium hyaluronate cross-polymer, and D-panthenol. The skin care product is enhanced by the penetration-promoting effect of the atomizer and / or the penetration-promoting device to promote skin absorption, achieve moisturizing effects, and increase the water content of the skin's stratum corneum.

[0024] The above description is only an overview of the technical solution of the present application. In order to more clearly understand the technical means of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0026] FIG1 is a schematic structural diagram of an atomizer provided in an embodiment of the present application;

[0027] FIG2 is a schematic flow chart of an embodiment of a method for controlling a permeation-promoting device provided by the present invention;

[0028] FIG3 is a comparison chart of cell proliferation activity test results;

[0029] FIG4 is a comparison diagram of the test structures of the first, second and third groups;

[0030] Figure 5 is a microscopic image of the skin care product after atomization;

[0031] Figure 6 is a microscopic image of the skin care product after application;

[0032] FIG7 is a diagram showing the penetration distribution of active ingredients in the skin under different usage modes;

[0033] FIG8 is the fluorescence quantitative data of active substances in the skin under different usage modes;

[0034] FIG9 is a diagram showing the fluorescence distribution of the active ingredient in the dermis under different usage modes;

[0035] FIG10 is the fluorescence quantitative data of active substances in the dermis under different usage modes. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical solution and effect of this application clearer and more specific, the following embodiments of the technical solution of this application will be described in detail with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of this application and are therefore only examples and are not intended to limit the scope of protection of this application.

[0037] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above-mentioned figure descriptions are intended to cover non-exclusive inclusions.

[0038] In the description of the embodiments of the present application, the technical terms "first" and "second" are used only to distinguish different objects and should not be understood as indicating or implying relative importance or implicitly indicating the quantity, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of the present application, the term "multiple" refers to more than two (including two), similarly, "multiple groups" refers to more than two (including two), and "multiple pieces" refers to more than two (including two), unless otherwise clearly and specifically defined.

[0039] References herein to "embodiments" mean that a particular feature, structure, or characteristic described in connection with the embodiments may be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor does it constitute an independent or alternative embodiment that is mutually exclusive of other embodiments. It is understood, both explicitly and implicitly, by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0040] In the description of the embodiments of this application, the term "and / or" is simply a description of the association relationship between associated objects, indicating that three relationships can exist. For example, A and / or B can represent the following three situations: A exists alone, A and B exist simultaneously, and B exists alone. In addition, the character " / " in this document generally indicates that the associated objects are in an "or" relationship.

[0041] Amounts, ratios, and other numerical values ​​are presented herein in a range format. It should be understood that such range format is used for convenience and brevity and should be interpreted flexibly to include not only the values ​​explicitly specified as range limits, but also all individual values ​​or sub-ranges encompassed within the range, as if each value and sub-range were explicitly specified.

[0042] If not otherwise specified, all steps of the present application may be performed sequentially, randomly, or in parallel, preferably sequentially. For example, the method includes steps (a) and (b), indicating that the method may include steps (a) and (b) performed sequentially, or may include steps (b) and (a) performed sequentially, or may include steps (a) and (b) performed simultaneously in parallel. For example, the method may further include step (c), indicating that step (c) may be added to the method in any order, for example, the method may include steps (a), (b), and (c), or may include steps (a), (c), and (b), or may include steps (c), (a), and (b), etc.

[0043] Skin is the body's first line of defense against external aggressors, helping to protect the body from harmful substances such as bacteria. Healthy, hydrated skin helps ensure that it constantly renews itself to maintain its protective barrier function.

[0044] Exfoliation is a normal metabolic process of keratinocytes, and our epidermal cells renew themselves approximately every 28 days. As we age, this process slows down, potentially to as little as 40 days. This slowdown can lead to skin problems such as roughness, sallowness, uneven skin tone, hyperpigmentation, fine lines, and wrinkles.

[0045] The water content of the stratum corneum is crucial for normal stratum corneum metabolism and skin desquamation. It participates in the enzymatic process of desquamation hydrolysis and also affects the activity of hydrolytic enzymes. If the water content of the stratum corneum falls below a critical level, the enzyme function required for normal desquamation is impaired, leading to keratinocyte adhesion and accumulation on the skin surface. These changes manifest as the physiological phenomena of dryness, roughness, and flaking of the skin.

[0046] Therefore, the water content of the stratum corneum is of great significance to the barrier function of the skin. Whether the skin care products have played a moisturizing function can be judged by whether the water content of the stratum corneum of the skin is increased. The higher the water content of the stratum corneum, the more conducive it is to improving the barrier function of the skin.

[0047] Based on this, the present application provides an application of a skin care active substance in the preparation of skin care products, which can achieve moisturizing of the skin, increase the water content of the skin's stratum corneum, enhance the skin's barrier function, and help protect the skin.

[0048] An embodiment of the present application provides an application of a skin care active in the preparation of a skin care product. Specifically, an atomizer and / or a penetration-promoting device are used to promote skin absorption of the skin care active; wherein the skin care active includes a moisturizer, and the moisturizer includes at least one of ceramide NP, hydrolyzed sodium hyaluronate, sodium hyaluronate, acetylated hyaluronic acid, sodium hyaluronate cross-linked polymer, and D-panthenol.

[0049] Ceramide NP is the primary building block of the skin barrier, stimulating the synthesis of endogenous sebum to moisturize and repair dry, aging skin. Hydrolyzed sodium hyaluronate, sodium hyaluronate, sodium hyaluronate crosspolymer, and acetylated sodium hyaluronate all act as skin hydrating factors, strengthening the skin barrier, locking in moisture, achieving moisturizing effects, and improving skin condition. Combining hydrolyzed sodium hyaluronate, sodium hyaluronate, sodium hyaluronate crosspolymer, and acetylated sodium hyaluronate creates multiple hyaluronic acid compounds of varying molecular weights, covering the skin's surface and deeper layers, providing intense moisturization and repairing the skin barrier. D-Panthenol significantly improves moisturizing and barrier repair in irritated skin, inhibiting the production of inflammatory factors and providing a soothing effect.

[0050] By designing the moisturizer of skin care active ingredients to include at least one of ceramide NP, hydrolyzed sodium hyaluronate, sodium hyaluronate, acetylated hyaluronic acid, sodium hyaluronate cross-polymer, and D-panthenol, the skin care product can achieve moisturizing while having certain repair effects and improving skin condition.

[0051] The skin care actives including moisturizers are atomized onto the skin surface by an atomizer; that is, the atomizer applies the skin care actives to the skin surface in the form of a spray. The atomization process reduces the viscosity of the skin care actives and increases the diffusion coefficient through the shear thinning phenomenon, which helps the skin care actives penetrate into the skin. Optionally, the atomized particles produced by the spray method have a D50 particle size of 10μm-50μm. Particles in this particle size range can effectively reach the pores and skin grooves of the skin, helping to improve the skin's absorption efficiency. Optionally, the atomized particles produced by the spray method have a D50 particle size of 10μm-30μm, which can effectively reach the pores and skin grooves of the skin, helping to improve the skin's absorption efficiency. In addition, compared to applying skin care products on the skin, using an atomizer to atomize the skin care products on the skin surface makes the skin care products more evenly distributed across the skin surface (the material will not be carried away by the application), achieving long-lasting moisturizing effects and increasing the water content of the skin's stratum corneum.

[0052] The penetration enhancer can be used to promote the penetration of skin care products applied to the skin surface, facilitating deep absorption into the skin and increasing the water content of the skin's stratum corneum. The penetration enhancer includes dual penetration enhancement functions, namely electroporation and iontophoresis. Electroporation disrupts the structure of the lipid bilayer in the stratum corneum by applying short, high-amplitude pulses, thereby creating temporary penetration pathways. These microchannels remain open for several minutes after the electric pulse, promoting transdermal absorption of active ingredients. Iontophoresis uses a low voltage and a low current density to drive charged active ingredients into the skin using charged electrodes. In addition, skin care actives are designed to contain ionic ingredients or charged encapsulated ingredients, for example, sodium hyaluronate, quintuple hyaluronic acid liposomes (quintuple hyaluronic acid liposomes include hydrolyzed sodium hyaluronate of different molecular weights and sodium hyaluronate of different molecular weights, sodium hyaluronate includes sodium hyaluronate with a molecular weight of 220 WDa, sodium hyaluronate with a molecular weight of 138 WDa, sodium hyaluronate with a molecular weight of 36 WDa, hydrolyzed sodium hyaluronate includes hydrolyzed sodium hyaluronate with a molecular weight of 3.9 WDa, and hydrolyzed sodium hyaluronate with a molecular weight of 7-8 KDa), which can synergize with the penetration enhancer to further improve the skin's absorption efficiency.

[0053] In one embodiment, the skin care actives further include at least one of a moisturizing and firming agent, a soothing and anti-sensitizing agent, an antioxidant, a soothing agent, and an anti-wrinkle and firming agent; the moisturizing and firming agent includes at least one of hydrolyzed lupin protein and XVII collagen; the soothing and anti-sensitizing agent includes peony root extract; the anti-wrinkle and firming agent includes at least one of acetyl hexapeptide-8, tripeptide-1, acetyl octapeptide-3, snake venom peptide, palmitoyl tripeptide-5, palmitoyl tripeptide-1, palmitoyl hexapeptide-12, acetyl tetrapeptide-11, and hexapeptide-3; the antioxidant includes at least one of ergothioneine, coenzyme Q10, VC derivatives, VE derivatives, VC glucoside, tocopheryl acetate, silymarin, and unicorn extract; the soothing agent includes at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol. By making the skin care active substance include at least one of a moisturizing and firming agent, a soothing and anti-sensitizing agent, an antioxidant, a soothing agent, and an anti-wrinkle and firming agent, the skin care effect of the skin care active substance is improved.

[0054] In one embodiment, the skin care active comprises, by weight, 3-90 parts by weight of an anti-wrinkle and firming agent, 0.1-15 parts by weight of a moisturizer, and 0.05-2.4 parts by weight of other ingredients; wherein the anti-wrinkle and firming agent comprises a first anti-wrinkle and firming agent and / or a second anti-wrinkle and firming agent, the first anti-wrinkle and firming agent comprises at least one of acetyl hexapeptide-8, tripeptide-1, acetyl octapeptide-3, and snake venom peptide; the second anti-wrinkle and firming agent comprises at least one of palmitoyl tripeptide-5, palmitoyl tripeptide-1, palmitoyl hexapeptide-12, acetyl tetrapeptide-11, and hexapeptide-3; and the moisturizer comprises at least one of ceramide NP, hydrolyzed sodium hyaluronate, and sodium hyaluronate. Other ingredients include 0.05-0.4 parts by weight of a moisturizing and firming agent and 0.05-2 parts by weight of at least one of a soothing and anti-allergic agent; wherein the moisturizing and firming agent includes at least one of hydrolyzed lupin protein and XVII collagen, and the soothing and anti-allergic agent includes peony root extract.

[0055] The first anti-wrinkle and firming agent acts on muscles, inhibiting muscle contraction and thus reducing the formation of wrinkles. For example, acetyl hexapeptide-8 can inhibit muscle contraction and thus reduce the formation of expression lines, thus achieving an anti-wrinkle effect and anti-aging through anti-wrinkle.

[0056] The second anti-wrinkle and firming agent acts on collagen in the skin, promoting its synthesis and maintenance, thereby reducing skin sagging and wrinkle formation. For example, palmitoyl tripeptide-5 can act on collagen in the skin, promoting its synthesis and maintenance, reducing skin sagging and wrinkle formation, thereby achieving an anti-wrinkle and firming effect. Simultaneously, palmitoyl tripeptide-5 can stimulate the proliferation of epidermal cells, promote the repair of the stratum corneum, and improve the skin's ability to retain moisture. In other words, palmitoyl tripeptide-5 has both anti-wrinkle and firming effects and moisturizing and repairing properties.

[0057] Ceramide NP is the main raw material for the brick wall structure of the skin barrier. It also stimulates the synthesis of endogenous sebum components to provide moisturizing and repair for dry and aging skin.

[0058] The functions of hydrolyzed sodium hyaluronate and sodium hyaluronate are to achieve moisturizing effects by strengthening the skin barrier and locking in moisture.

[0059] Moisturizing and firming agents stimulate collagen synthesis within cells, improving skin elasticity and firmness. They also have moisturizing properties, increasing skin moisture levels. For example, hydrolyzed lupin protein primarily promotes collagen production and skin firmness. This stimulates collagen synthesis within cells, improving skin elasticity and firmness. It also has moisturizing properties, increasing skin moisture levels and improving dryness and roughness.

[0060] Soothing and anti-allergic agents achieve soothing and anti-allergic effects through mitochondrial autophagy. For example, peony root extract optimizes cellular energy efficiency through mitochondrial autophagy, achieving soothing and anti-allergic effects, thereby combating skin aging.

[0061] By designing the skin care active ingredients to include at least one of a first anti-wrinkle and firming agent with anti-aging effects, a second anti-wrinkle and firming agent, and a soothing and anti-allergic agent, an anti-aging effect is achieved; by designing the skin care active ingredients to include at least one of a first anti-wrinkle and firming agent with firming effects, a second anti-wrinkle and firming agent, and a moisturizing and firming agent, a firming effect is achieved; and by designing the skin care active ingredients to include at least one of ceramide NP, hydrolyzed sodium hyaluronate, and sodium hyaluronate, a moisturizing effect is achieved. It can be understood that by designing the ingredients of the skin care active ingredients as described above, the skin care active ingredients simultaneously have anti-aging, firming, and moisturizing effects, effectively protecting the skin barrier and maintaining skin health. Furthermore, by designing the weight ratios of the various components of the skin care active ingredients as described above, the skin care active ingredients can act on the skin to achieve anti-wrinkle, firming, and moisturizing effects, with long-lasting effects without burdening the skin. This is highly safe, and it gently and effectively achieves anti-wrinkle, firming, and moisturizing effects, promoting a healthy skin state.

[0062] Optionally, the skin care active is composed of the following components in the following weight ratio: 3-10 parts by weight of acetyl hexapeptide-8, 3-10 parts by weight of palmitoyl tripeptide-5, 0.05-0.2 parts by weight of hydrolyzed lupin protein, 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, and 0.05-2 parts by weight of peony root extract.

[0063] Wherein, 0.1-10 weight parts of hydrolyzed sodium hyaluronate and sodium hyaluronate refer to the total weight parts of hydrolyzed sodium hyaluronate and sodium hyaluronate in the skin care active material is 0.1-10 weight parts. In 0.1-10 weight parts of hydrolyzed sodium hyaluronate and sodium hyaluronate, the weight ratio of hydrolyzed sodium hyaluronate to sodium hyaluronate is (2.5-4): (1.5-3). Exemplary, in 0.1-10 weight parts of hydrolyzed sodium hyaluronate and sodium hyaluronate, sodium hyaluronate includes sodium hyaluronate with a molecular weight of 220 WDa, sodium hyaluronate with a molecular weight of 138 WDa, and sodium hyaluronate with a molecular weight of 36 WDa, and hydrolyzed sodium hyaluronate includes hydrolyzed sodium hyaluronate with a molecular weight of 3.9 WDa and hydrolyzed sodium hyaluronate with a molecular weight of 7-8 KDa. 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate contain hyaluronic acid of various molecular weights, which covers the skin surface to the deep layers of the skin, strengthens the skin barrier function, locks in moisture, and achieves a moisturizing effect. Exemplarily, in 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, the weight ratio of hydrolyzed sodium hyaluronate to sodium hyaluronate can be 3.5:2.5, 3:2.5, 3:2, 3.5:2, etc. The 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate in the following content are the same as above and will not be repeated here.

[0064] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, 3-10 parts by weight of acetyl hexapeptide-8, 3-10 parts by weight of palmitoyl tripeptide-5, and 0.05-0.2 parts by weight of hydrolyzed lupin protein.

[0065] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 3-10 parts by weight of acetyl hexapeptide-8, 3-10 parts by weight of palmitoyl tripeptide-5, and 0.05-0.2 parts by weight of hydrolyzed lupin protein.

[0066] Optionally, the skin care active ingredient is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of sodium hyaluronate, 3-10 parts by weight of palmitoyl tripeptide-5, and 0.05-0.2 parts by weight of hydrolyzed lupin protein.

[0067] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, 0.05-2 parts by weight of peony root extract, and 3-10 parts by weight of acetyl hexapeptide-8.

[0068] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, 0.05-2 parts by weight of peony root extract, and 3-10 parts by weight of palmitoyl tripeptide-5.

[0069] In one embodiment, the skin care active ingredients include at least two of 0.0001-12 parts by weight of an antioxidant, 0.05-0.4 parts by weight of a moisturizing and firming agent, 0.05-2 parts by weight of a soothing and anti-allergic agent, and 0.1-15 parts by weight of a moisturizer, in a weight ratio; wherein the antioxidant includes at least one of ergothioneine, coenzyme Q10, a VC derivative, and a VE derivative; the moisturizing and firming agent includes at least one of hydrolyzed lupine protein and type XVII collagen; the soothing and anti-allergic agent includes peony root extract; and the moisturizer includes at least one of ceramide NP, hydrolyzed sodium hyaluronate, and sodium hyaluronate.

[0070] Among them, antioxidants can intelligently target mitochondria and the cell nucleus, providing long-lasting antioxidant effects. For example, ergothioneine is a long-lasting antioxidant that intelligently targets mitochondria and the cell nucleus, exhibiting certain anti-aging effects and improving skin condition.

[0071] The main function of moisturizing and firming agents is to promote collagen production and skin firmness, while also increasing skin moisture content. For example, hydrolyzed lupin protein stimulates collagen synthesis within cells, improving skin elasticity and firmness. It also has moisturizing properties, increasing skin moisture content, improving dryness and roughness, and improving skin condition.

[0072] Soothing and anti-allergic agents achieve soothing and anti-allergic effects through mitochondrial autophagy. For example, peony root extract optimizes cellular energy efficiency through mitochondrial autophagy, achieving soothing and anti-allergic effects, thereby resisting skin aging and improving skin condition.

[0073] Ceramide NP is the main raw material for the brick wall structure of the skin barrier. It also stimulates the synthesis of endogenous sebum components, provides moisturizing and repair for dry and aging skin, and thus improves skin condition.

[0074] The functions of hydrolyzed sodium hyaluronate and sodium hyaluronate are to strengthen the skin barrier, lock in moisture, achieve moisturizing effects, and improve skin condition.

[0075] Improvement of skin condition is achieved by designing skin care active ingredients to include at least two of the following: antioxidants, moisturizing and firming agents, soothing and anti-allergic agents, and moisturizers that have the effect of improving skin condition. Specifically, antioxidants, moisturizing and firming agents, soothing and anti-allergic agents, and moisturizers improve skin condition from different aspects respectively. At least two of the antioxidants, moisturizing and firming agents, soothing and anti-allergic agents, and moisturizers are selected for combination to improve skin condition from multiple angles. The action pathways of skin care active ingredients are diverse, and the skin condition is improved gently and efficiently. In addition, the weight proportions of the various components of the skin care active ingredients are designed as above, so that the skin care active ingredients can act on the skin to have the effects of moisturizing, soothing, repairing, and anti-oxidation, and the effects are long-lasting, while not burdening the skin. It is safer, gently and efficiently improves skin condition, effectively protects the skin barrier, and maintains skin health.

[0076] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.0001-3 parts by weight of ergothioneine, 0.05-0.2 parts by weight of hydrolyzed lupin protein, 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, and 0.05-2 parts by weight of peony root extract.

[0077] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, and 0.0001-3 parts by weight of ergothioneine.

[0078] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate, 0.05-2 parts by weight of peony root extract, and 0.05-0.2 parts by weight of hydrolyzed lupin protein.

[0079] Optionally, the skin care active ingredient is composed of the following components in the following weight ratio: 0.05-2 parts by weight of peony root extract, 0.05-0.2 parts by weight of hydrolyzed lupin protein, and 0.0001-3 parts by weight of ergothioneine.

[0080] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, and 0.0001-3 parts by weight of ergothioneine.

[0081] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, and 0.05-0.2 parts by weight of hydrolyzed lupin protein.

[0082] In one embodiment, the skin care active ingredients include, by weight, 0.31-13 parts of an antioxidant and 0.1-10 parts of a moisturizer. The antioxidant includes 0.1-5 parts of VC glucoside, 0.1-2 parts of tocopheryl acetate, 0.01-1 parts of silymarin, and 0.1-5 parts of unicorn extract. The moisturizer includes at least one of sodium hyaluronate crosspolymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate.

[0083] Among them, VC glucoside can effectively resist oxidation, remove yellowness and brighten skin. Tocopheryl acetate has strong reducing properties, has antioxidant effects, and scavenges free radicals. Silymarin has extremely strong antioxidant and free radical scavenging activity, promoting the production of collagen and hyaluronic acid. Kirin extract can improve microcirculation and has anti-inflammatory and antioxidant effects. Hydrolyzed sodium hyaluronate, sodium hyaluronate, sodium hyaluronate crosspolymer, and acetylated sodium hyaluronate all act as skin hydrating factors, which can strengthen the skin barrier, lock in moisture, achieve moisturizing effects, and improve skin condition. Hydrolyzed sodium hyaluronate, sodium hyaluronate, sodium hyaluronate crosspolymer, and acetylated sodium hyaluronate combine to form multiple hyaluronic acids of different molecular weights, covering the skin surface to the deep layers of the skin, effectively moisturizing and repairing the skin barrier.

[0084] By designing skincare actives to include multiple antioxidants with antioxidant properties, they deliver antioxidant benefits to the skin through multiple pathways, synergizing to achieve a highly effective and gentle antioxidant effect, improving skin condition and maintaining skin health. Furthermore, by designing the weight ratios of the various components of the skincare actives as described above, the skincare actives deliver long-lasting antioxidant and moisturizing benefits without burdening the skin, resulting in a safer, gentler, and more effective approach to improving skin condition, effectively protecting the skin barrier, and maintaining skin health.

[0085] Optionally, the skin care active comprises 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate. The 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate refer to the total weight of these four substances in the skin care active is 0.1-10 parts by weight. Among the 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate, the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2). It is understood that when the moisturizing agent of the skin care active comprises sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the skin care active comprises 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate. Exemplarily, in the 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate, the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate can be 0.3:0.5:1:0.15, 0.2:0.7:0.8:0.1, 0.5:0.8:1.2:0.2, 0.4:1:1:0.1, etc. The weight ratios of 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate in the following content are the same as above and will not be repeated herein.

[0086] Exemplary, skin care actives include: sodium hyaluronate cross-linked polymers with a molecular weight greater than 3000KDa (kilodaltons), sodium hyaluronate with a molecular weight of 1000-1800KDa, acetylated sodium hyaluronate with a molecular weight of 1000KDa, and hydrolyzed sodium hyaluronate with a molecular weight of less than 10KDa. By designing the molecular weights of sodium hyaluronate cross-linked polymers, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the skin is covered from the surface layer to the deep layer of the skin, the skin barrier function is strengthened, moisture is locked, and a good moisturizing effect is achieved. It should be noted that when the skin care active moisturizer is composed of sodium hyaluronate cross-linked polymers, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the molecular weight selection of the above-mentioned sodium hyaluronate cross-linked polymers, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is also applicable to the content introduced below.

[0087] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of sodium hyaluronate cross-polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate.

[0088] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of hydrolyzed sodium hyaluronate.

[0089] Optionally, the skin care active ingredient is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate. The 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate means that the total weight of these three substances in the skin care active ingredient is 0.1-10 parts by weight. The weight ratio of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1. For example, in 0.1-10 parts by weight of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the weight ratio of sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate can be 4.5:5.5:1, 5:6:1, 5.5:6.5:1, etc.

[0090] Optionally, the skin care active comprises the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate. The 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate means that the total weight of these three substances in the skin care active is 0.1-10 parts by weight. The weight ratio of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1. For example, in 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, the ratio of sodium hyaluronate cross-linked polymer, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate can be 4.5:5.5:1, 5:6:1, 5.5:6.5:1, etc.

[0091] Optionally, the skin care active comprises the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate. The 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate means that the total weight of these three substances in the skin care active is 0.1-10 parts by weight. The weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate is (4.5-5.5):(5.5-6.5):1. Illustratively, in 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate, the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, and hydrolyzed sodium hyaluronate can be 4.5:5.5:1, 5:6:1, 5.5:6.5:1, etc.

[0092] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of unicorn extract, and 0.1-10 parts by weight of sodium hyaluronate.

[0093] In one embodiment, the skin care active comprises, by weight, 0.1-15 parts by weight of a moisturizer and 0.1-12 parts by weight of a soothing agent; wherein the moisturizer comprises at least one of quadruple hyaluronic acid and D-panthenol, and the quadruple hyaluronic acid comprises sodium hyaluronate crosspolymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate; and the soothing agent comprises at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol.

[0094] Quadruple hyaluronic acid acts as a skin hydrating factor, with multiple hyaluronic acids of varying molecular weights providing intense moisturization and repairing the skin barrier. D-panthenol significantly moisturizes and repairs the skin barrier in irritated skin, inhibiting the production of inflammatory factors and providing a soothing effect. Portulaca oleracea extract exhibits potent anti-inflammatory activity and soothes the skin. Kava kava extract also soothes and inhibits TRPV-1.

[0095] By designing skincare actives to include at least one of quadruple hyaluronic acid and D-panthenol, and at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol, this multifaceted approach simultaneously addresses both skin inflammation and allergies, as well as barrier reconstruction. This synergistic approach achieves highly effective and gentle moisturizing, repairing, and soothing effects, improving skin condition and maintaining skin health. Furthermore, the weight ratio of the moisturizer and soothing agent in the skincare actives is designed as described above, ensuring that the skincare actives deliver long-lasting moisturizing, repairing, and soothing effects on the skin without burdening the skin. This results in a highly safe, gentle, and effective improvement of skin condition, effectively protecting the skin barrier, and maintaining skin health.

[0096] Optionally, the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate in the 4-fold hyaluronic acid is (0.2-0.5): (0.5-1): (0.5-1.2): (0.1-0.2). Exemplary, skin care actives include: a sodium hyaluronate cross-linked polymer with a molecular weight greater than 3000KDa (kilodaltons), a sodium hyaluronate with a molecular weight of 1000-1800KDa, an acetylated sodium hyaluronate with a molecular weight of 1000KDa, and a hydrolyzed sodium hyaluronate with a molecular weight less than 10KDa. By designing the molecular weight of the sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate as described above, the skin surface is covered to the deep layer of the skin, the skin barrier function is strengthened, moisture is locked, and a good moisturizing effect is achieved. The components of the 4-fold hyaluronic acid in this application are all the same as these and will not be repeated here.

[0097] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of quadruple hyaluronic acid, 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, 0.1-1 parts of kava extract, and 0.1-5 parts by weight of unicorn extract.

[0098] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of quadruple hyaluronic acid, 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, and 0.1-1 part of kava extract.

[0099] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of quadruple hyaluronic acid, 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, and 0.1-5 parts by weight of unicorn extract.

[0100] Optionally, the skin care active ingredient is composed of the following components in the following weight ratio: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts of D-panthenol, 0.1-1 parts of kava extract, and 0.1-5 parts by weight of unicorn extract.

[0101] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of quadruple hyaluronic acid, 0.5-5 parts by weight of purslane extract, 0.1-1 parts of kava extract, and 0.1-5 parts by weight of unicorn extract.

[0102] Optionally, the skin care active ingredient is composed of the following components in the following weight ratio: 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of Portulaca oleracea extract, 0.1-1 parts of Kava kava extract, and 0.1-5 parts by weight of Kirindra chinensis extract.

[0103] Optionally, the skin care active ingredient is composed of the following components in the following weight ratio: 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, and 0.1-1 parts of kava extract.

[0104] In one embodiment, the skin care active ingredients include 0.1-15 parts by weight of a moisturizer and 0.05-2 parts by weight of a soothing and anti-allergic agent, wherein the moisturizer includes at least two of ceramide NP, hydrolyzed sodium hyaluronate, and sodium hyaluronate; and the soothing and anti-allergic agent includes peony root extract.

[0105] Ceramide NP is the primary building block of the skin barrier's brick wall structure, stimulating the synthesis of endogenous sebum components to moisturize and repair dry, aging skin. Both hydrolyzed sodium hyaluronate and sodium hyaluronate achieve moisturizing effects by strengthening the skin barrier and locking in moisture. The combination of hydrolyzed sodium hyaluronate and sodium hyaluronate, which contain hyaluronic acid of varying molecular weights, covers the skin from the surface to the deeper layers, strengthening the skin's barrier function and locking in moisture for a moisturizing effect. Paeonia lactiflora root extract optimizes cellular energy efficiency through mitochondrial autophagy, combating skin aging.

[0106] By designing skincare actives to include at least two of ceramide NP, hydrolyzed sodium hyaluronate, and sodium hyaluronate, along with peony root extract, they synergize to achieve highly effective and gentle moisturizing and soothing effects, improving skin condition and maintaining skin health. Furthermore, by designing the weight ratios of the moisturizer and soothing anti-allergic agent in the skincare actives as described above, the skincare actives deliver long-lasting moisturizing and soothing effects on the skin without burdening the skin, resulting in a highly safe formulation that gently and efficiently improves skin condition, effectively protects the skin barrier, and maintains skin health.

[0107] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, 0.05-2 parts by weight of peony root extract, and 0.01-5 parts by weight of acetyl glucosamine.

[0108] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, and 0.05-2 parts by weight of peony root extract.

[0109] Optionally, the skin care active ingredient is composed of the following components in the following weight ratio: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate, and 0.05-2 parts by weight of peony root extract.

[0110] Optionally, the skin care active ingredient is composed of the following components in the following weight ratios: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of sodium hyaluronate, 0.05-2 parts by weight of peony root extract

[0111] Optionally, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, 0.05-2 parts by weight of peony root extract, and 0.01-5 parts by weight of acetyl glucosamine.

[0112] In addition to the skin care active ingredients described above, skin care products also include a base and deionized water. The base typically includes ingredients with moisturizing properties. The dosage form of the skin care product includes any one of an aqueous solution, emulsion, cream, essence lotion, mask, or spray. Optionally, the base includes glycerin, polydimethylsiloxane, squalane, acrylates / C10-30 alkyl acrylate crosspolymer, and polysorbate 60; in this case, the dosage form of the skin care product 120 is an essence lotion.

[0113] Please refer to Figure 1, which is a structural diagram of the atomizer provided in an embodiment of the present application.

[0114] An embodiment of the present application provides an atomizer. The atomizer has a receiving chamber 201. A skincare product is filled in the receiving chamber 201. The atomizer atomizes the skincare active ingredient of any of the above-described embodiments onto the skin surface, distributing the skincare product more evenly across the skin surface and facilitating skin absorption, thereby achieving a moisturizing effect and increasing the water content of the skin's stratum corneum.

[0115] Optionally, the atomization principle of the nebulizer provided in the embodiment of the present application is "two-phase flow", which can evenly atomize skin care products on the skin. "Two-phase flow" nebulizers usually use compressed air to collide and mix with liquid through a special nozzle structure. When the aerodynamic external force on the liquid is greater than the surface tension and viscosity of the liquid itself, the original stable agglomeration state of the liquid will be broken, and the continuous liquid mass will be broken up into a discontinuous group of tiny droplets, eventually forming a spray phenomenon of gas-liquid two-phase mixture. It should be noted that the atomization principle of the nebulizer is not limited to "two-phase flow", and the atomized particles generated by the spray method only need to have a D50 particle size of 10μm-50μm to improve the skin absorption efficiency.

[0116] The penetration-promoting device provided in the embodiment of the present application is a multifunctional instrument that integrates "electroporation + iontophoresis + EMS", which can effectively promote the penetration of skin care products on the skin.

[0117] This embodiment provides a penetration-promoting device, which includes at least one electrode group, and the electrode group is used to output a penetration-promoting electric field. The penetration-promoting electric field can promote the penetration and absorption of the permeation medium on the user's skin or achieve a cosmetic effect, depending on the frequency and magnitude. Specifically, it can achieve radiofrequency beauty, EMS (Electrical Muscle Stimulation) microcurrent beauty, ion introduction, electroporation, etc. The electrode group includes at least two adjacent electrodes, and an electric field for penetration promotion or cosmetic effect can be formed between the two adjacent electrodes.

[0118] In one embodiment, the permeation promoting device may include at least two electrodes, which constitute an electrode group.

[0119] In a specific embodiment, the electrode group can output a low-frequency current, and the current output frequency range of the electrode group is not less than 30kHz and not more than 50kHz, and the corresponding peak-to-peak value range is 30Vpp to 56Vpp. Specifically, the current output frequency corresponding to the electrode group can be 32kHz, and the corresponding peak-to-peak value range is 32Vpp to 40Vpp. The current output frequency corresponding to the electrode group can be 50kHz, and the corresponding peak-to-peak value range is 40Vpp to 56Vpp. The current output frequency corresponding to the electrode group can be 40kHz, and the corresponding peak-to-peak value range is 32Vpp to 48Vpp.

[0120] In another specific embodiment, the electrode assembly can output a high-frequency current, wherein the current output frequency of the electrode assembly is greater than 100 kHz and less than 400 kHz, and the corresponding peak-to-peak value range is 60 Vpp to 70 Vpp. Specifically, the current frequency corresponding to the electrode assembly is 127 kHz, and the corresponding peak-to-peak value does not exceed 64 Vpp.

[0121] Please refer to FIG. 2 , which is a flow chart of an embodiment of a control method for a permeation-promoting device provided by the present invention.

[0122] The present application provides a control method for a permeation-enhancing device, and the control method provided in this embodiment is applicable to the permeation-enhancing device provided in the above embodiments. The control method for the permeation-enhancing device includes the following steps.

[0123] S1: Electrically stimulate the target area based on the electrode groups corresponding to the various penetration-enhancing modes.

[0124] Specifically, there are at least two penetration-enhancing modes in this embodiment, and at least some of the operating parameters between the penetration-enhancing modes are different. Among them, at least one penetration-enhancing mode corresponds to a first operating parameter, and the current output frequency range of the first operating parameter is not less than 30kHz and not more than 50kHz, and the corresponding peak-to-peak range is 30Vpp to 56Vpp. At least one penetration-enhancing mode corresponds to a second operating parameter, and the current output frequency range of the second operating parameter is greater than 100kHz and less than 400kHz, and the corresponding peak-to-peak range is 60Vpp to 70Vpp. Based on low-frequency current and high-frequency current, the electrode group can be made to perform electrode on the target site under different voltage conditions to achieve shallow penetration and deep penetration.

[0125] The penetration-promoting device in the present application electrically stimulates the target area based on the electrode groups corresponding to the various penetration-promoting modes, thereby achieving a variety of different penetration effects and body sensations, thereby realizing the diversity of the penetration functions of the penetration-promoting device.

[0126] The present application also provides a method for preparing a skin care product.

[0127] In a first embodiment, the method for preparing a skin care product specifically comprises:

[0128] Step S01: Atomize a skin care product toward the skin surface using an atomizer to form a liquid film on the skin surface; wherein the skin care active ingredient of the skin care product includes a moisturizer, and the moisturizer includes at least one of ceramide NP, hydrolyzed sodium hyaluronate, sodium hyaluronate, acetylated hyaluronic acid, sodium hyaluronate cross-linked polymer, and D-panthenol.

[0129] Step S02: using a penetration-promoting device to promote penetration of the liquid film on the skin surface.

[0130] It is understood that the specific ingredients of the skin care active ingredients of the skin care products used in this skin care method can be found in the relevant description of the above embodiments and will not be repeated here. Using an atomizer and a penetration-enhancing device for skin care, the skin care products themselves have moisturizing effects. With the cooperation of the atomizer and the penetration-enhancing device, the water content of the skin's stratum corneum can be greatly increased, which helps to protect the skin.

[0131] In a second embodiment, the method for preparing a skin care product specifically comprises:

[0132] Step S00: Apply moisturizing water to the skin surface. The moisturizing water is composed of water, dipropylene glycol, glycerin, polyethylene glycol, glycosyl trehalose, phenoxyethanol, hydrogenated starch hydrolysate, sodium citrate, citric acid, allantoin, sodium PCA, butylene glycol, polyquaternium-51, grapefruit seed extract, and purslane extract.

[0133] Step S01: Atomize a skin care product toward the skin surface using an atomizer to form a liquid film on the skin surface; wherein the skin care active ingredient of the skin care product includes a moisturizer, and the moisturizer includes at least one of ceramide NP, hydrolyzed sodium hyaluronate, sodium hyaluronate, acetylated hyaluronic acid, sodium hyaluronate cross-linked polymer, and D-panthenol.

[0134] Step S02: using a penetration-promoting device to promote penetration of the liquid film on the skin surface.

[0135] It is understood that the specific ingredients and ratios of the skin care active ingredients used in this method can be found in the description of the atomizer in the above embodiment and will not be repeated here. Applying moisturizing lotion first and then using the atomizer and penetration-enhancing device for skin care will facilitate the penetration of active ingredients, increase the water content of the skin's stratum corneum, and further enhance the moisturizing effect.

[0136] The skin care actives provided herein have moisturizing effects, and relevant experimental tests and studies have been carried out at the cellular level, the human body level, and the in vitro pigskin penetration level. Below, a skin care product is composed of the following components in the following weight ratios: 1.5 parts by weight of ceramide NP, 1 part by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate (wherein, hydrolyzed hyaluronic acid is 0.6 part by weight, and sodium hyaluronate is 0.4 part by weight), 0.4 part by weight of peony root extract, 0.1 part by weight of acetylglucosamine, 5 parts by weight of glycerin, 2.4 parts by weight of polydimethylsiloxane, 2 parts by weight of squalane, 0.8 part by weight of C10-30 alkyl acrylate crosspolymer, 1.3 parts by weight of polysorbate 60, and 85.5 parts by weight of deionized water. Experimental tests on the cellular level, the human body level, and the in vitro pigskin penetration have been carried out.

[0137] 1. Cell proliferation activity assay.

[0138] Experimental steps:

[0139] (1) HSF cells were seeded into 96-well plates, and 100 μL of culture medium containing 8*103 HSF cells was added to each well. The plates were incubated at 37°C for 24 h until the cells adhered to the plate and entered the logarithmic growth phase.

[0140] (2) After 24 hours, the culture medium in step (1) was discarded, and 100 μL of the test solution of different concentrations was added to each well. Three parallel wells were set for each concentration, and the cells were incubated for 24 hours.

[0141] (3) Aspirate the test solution added in step (2), add 90 μL of fresh culture medium and 10 μL of CCK-8 solution (Cell Counting Kit-8 reagent), and incubate for 1 to 4 hours.

[0142] (4) Measure the absorbance at 450 nm using an enzyme-labeled instrument.

[0143] Calculation method: Cell viability*(%)=[A(sample added)-A(blank) / [A(0 sample added)-A(blank)]×100;

[0144] Wherein, A(sample added) represents the absorbance of the wells containing cells, CCK-8 solution, and test solution;

[0145] A (blank) indicates the absorbance of wells with culture medium and CCK-8 solution but no cells;

[0146] A(0 loading) represents the absorbance of the wells containing cells and CCK-8 solution but no test solution;

[0147] Cell viability* indicates cell proliferation activity or cytotoxic activity.

[0148] It should be noted that when adding the culture medium containing CCK-8 to the cells in step (3), several blank wells without cells are added simultaneously to correct the values, which are referred to as A (blank). In step (2), several wells do not contain the test solution, but only the cell culture medium, which are referred to as A (0 addition).

[0149] Experimental results:

[0150] The experimental results are shown in Figure 3, which is a comparison chart of the cell proliferation activity test results. According to Figure 3, for the skin care active ingredient, cell activity tests were conducted on cells using concentrations of 10%, 5%, 1%, 0.1%, 0.05%, 0.001%, and 0, respectively. The concentration here refers to the concentration of the skin care product diluted with cell culture medium, i.e., the "different concentrations of test solution" in step (2). At concentrations of 0.05% and 0.1%, excellent proliferation activity was expressed, and the cell activity at a concentration of 0 increased by 15.99% and 8.79%, respectively.

[0151] The cell activity test proves that the skin care product provided in this application has a good effect of promoting cell proliferation and can effectively protect the skin.

[0152] 2. Human efficacy experiments.

[0153] 1. Experimental equipment and consumables.

[0154] (1) Delfin moisture meter.

[0155] (2) The atomizer provided in this application.

[0156] (3) The penetration-promoting device provided in this application.

[0157] (4) The skin care products provided by this application.

[0158] (5) MUJI Fresh Basic Moisturizing Water (referred to as "basic water", MUJI Fresh Basic Moisturizing Water includes water, dipropylene glycol, glycerin, polyethylene glycol, glycosyl trehalose, phenoxyethanol, hydrogenated starch hydrolysate, sodium citrate, citric acid, allantoin, sodium PCA, butylene glycol, polyquaternium-51, grapefruit seed extract, purslane extract).

[0159] (6) High-power microscope.

[0160] 2. Experimental groups.

[0161] The subjects in the human efficacy experiment were divided into three groups according to different testing methods, with 5 people in each group.

[0162] Group 1: Use skin care products on the left face by misting, and on the right face by applying skin care products.

[0163] Group 2: First, use basic water on the entire face, then mist the skincare product on the left side of the face, and then apply the skincare product on the right side of the face;

[0164] The third group: spray the skin care products on the left face and apply them on the right face, and finally use the penetration-enhancing device for 3 minutes.

[0165] 3. Test time point.

[0166] The test cycle was 14 days. During these 14 days, the stratum corneum moisture content of three groups of subjects was tested at different time periods: T0 (the initial time before taking the test sample), T4 (the 4th hour after taking the test sample), D3 (the 3rd day after taking the test sample), D7 (the 7th day after taking the test sample), and D14 (the 14th day after taking the test sample).

[0167] 4. The process of using skin care products.

[0168] Group 1:

[0169] Frequency of using skin care products: once in the morning and once in the evening.

[0170] Usage process:

[0171] (1) Cleanse your skin.

[0172] (2) Use an atomizer to atomize the skin care product. Specifically, turn on the atomizer, hold the device 10cm-15cm, move slowly, and spray the skin care product evenly on the left side of the face; use it for 30 seconds each time.

[0173] (3) Apply skin care products. Specifically, pump 2 pumps of skin care products into the palm of your hand and apply evenly on the right side of your face.

[0174] (4) Use sunscreen / isolation in the morning, or take physical protective measures (for example, wear a hat, hold an umbrella, etc.).

[0175] Group 2:

[0176] Frequency of using skin care products: once in the morning and once in the evening.

[0177] Usage process:

[0178] (1) Cleanse your skin.

[0179] (2) Apply foundation water all over the face. Specifically, pump 3 pumps of foundation water into the palm of your hand and apply evenly to the entire face.

[0180] (3) Use an atomizer to atomize the skin care product. Specifically, turn on the atomizer, hold the device 10cm-15cm, move slowly, and spray the skin care product evenly on the left side of the face; use it for 30 seconds each time.

[0181] (4) Apply skin care products. Specifically, pump 2 pumps of skin care products into the palm of your hand and apply evenly on the right side of your face.

[0182] (5) Use sunscreen / isolation in the morning, or take physical protective measures (for example, wear a hat, hold an umbrella, etc.).

[0183] Group 3:

[0184] Frequency of using skin care products: once in the morning and once in the evening.

[0185] Usage process:

[0186] (1) Cleanse your skin.

[0187] (2) Use an atomizer to atomize the skin care product. Specifically, turn on the atomizer, hold the device 10cm-15cm, move slowly, and spray the skin care product evenly on the left side of the face; use it for 30 seconds each time.

[0188] (3) Apply skin care products. Specifically, pump 2 pumps of skin care products into the palm of your hand and apply evenly on the right side of your face.

[0189] (4) Use a penetration-enhancing device. Specifically, use the penetration-enhancing device in the "enhanced penetration" mode, adjusting the gear according to the tester's comfort. Use a pressing and stamping method, pausing for 3 seconds on each part until all parts of the face are stamped and penetrated. It should be noted that the penetration-enhancing device should be used every other day.

[0190] (5) Use sunscreen / isolation in the morning, or take protective measures (for example, wear a hat, hold an umbrella, etc.).

[0191] 5. Requirements during the test.

[0192] (1) The usage period is 14 days.

[0193] (2) During the 14-day product testing process, only the test product should be used. No other skin care products or other skin care behaviors such as light medical beauty treatments should be used. (Cleansing and sunscreen products may be used during the trial; sun protection measures must be taken.)

[0194] (3) Use the same amount of skin care product on both the left and right faces. Skin care product usage: Mist on the left face for 30 seconds, then apply 2 pumps on the right face. Basic water usage: Apply 3 pumps on the entire face.

[0195] (4) The stratum corneum water content was measured on D0 (T0), D0 (T4), D3, D7, and D14 respectively (except for T4h, each test required facial cleansing and waiting in a constant temperature and humidity room for 20 minutes before conducting relevant tests).

[0196] Experimental results:

[0197] Table 1 The first set of test results

[0198] Table 2 The second set of test results

[0199] Table 3 The third set of test results

[0200] A comparison of the test structures of the first, second, and third groups is shown in Figure 4. The bar graphs corresponding to each test subject in Figure 4, from left to right, are T0 stratum corneum water content, T4 stratum corneum water content, D3 stratum corneum water content, D7 stratum corneum water content, D14 stratum corneum water content, T4 water content change rate, D3 water content change rate, D7 water content change rate, and D14 water content change rate.

[0201] According to Tables 1-3 and Figure 4, when the samples on the subjects' faces were not washed off after 4 hours of use, the three groups generally showed that the stratum corneum moisture increase rate under the atomization method was better than the application method; and the stratum corneum moisture increase rate under the atomization method of the second group (first using basic water, then atomizing the skin care product on the left face, and applying the skin care product on the right face) was the best.

[0202] After 3 days of use, the medium on the subjects' faces was washed off, and the subjects waited for 20 minutes under constant temperature and humidity conditions. The three groups generally showed that the rate of increasing stratum corneum water content under atomization was better than that under application; and the second group (first using basic water, then atomizing the composition on the left face, and applying the composition on the right face) had the best rate of increasing stratum corneum water content under atomization.

[0203] By comparing the data of the third group with those of the first group (the only difference is whether or not the penetration-enhancing device was used to enhance penetration), the advantage of penetration enhancement in the smearing mode is not obvious, while penetration enhancement in the atomization mode can increase the water content enhancement rate by 14%.

[0204] The data of the second group (first use base water, then spray the skin care product on the left face, and apply the skin care product on the right face) showed that the stratum corneum moisture content increase rate of the spray method was 31% higher than that of the application method, while the difference between the spray and application data of the first group was 18%; and the moisture content increase rate of the second group was 29% and 16% higher than that of the first group in the spray and application methods respectively, which further verifies that using base water before spraying is beneficial to increasing the moisture content.

[0205] According to the test results of the first, second and third groups, it is shown that the water content increase rate of atomization is better than that of application. During the application process, a certain amount of sample will be wiped away, and the amount of sample remaining on the face is relatively small. The penetration promotion cannot play its advantage, which affects the performance of the water content of the stratum corneum.

[0206] When the appropriate amount of sample is used, atomization results in a higher retention rate on the face than application, demonstrating a certain benefit in increasing hydration. Furthermore, atomization easily forms a uniform "film" on the skin. The presence of fluid water beneath this "film" promotes the penetration of skincare products, further increasing hydration.

[0207] The present applicant also observed arm skin under a high-power microscope after atomization and application of the skincare product, as shown in Figures 5 and 6, respectively. Figure 5 shows a microscopic image of the skincare product after atomization, and Figure 6 shows a microscopic image of the skincare product after application. Using an atomizer, the two-phase flow atomization method can more evenly cover the skin surface with the skincare product, forming a more uniform film. While some skincare product will be removed during application, some will remain in the furrows.

[0208] Based on tests at the human level, through 14 days of monitoring the water content of the stratum corneum of subjects under three usage methods, it can be generally concluded that the skin care product itself has good moisturizing effect, and the use of an atomizer and a penetration-promoting device can effectively promote the moisturizing effect of the skin care product, achieving synergistic enhancement of the material and the instrument.

[0209] 3. In vitro pigskin penetration experiment

[0210] 1. Test system:

[0211] Bama fragrant pig skin-Franz cell diffusion cell system, TK-12D transdermal absorption diffusion instrument (Shanghai Kaikai), and normal saline.

[0212] 2. Sample information

[0213] a. Instrument: Nebulizer (atomized particle size: D50 = 13.9 μm, D90 = 30.58 μm), penetration-enhancing device

[0214] b. Medium composition: Penta-hyaluronic acid liposomes (labeled with rhodamine b)

[0215] 3. Test Grouping

[0216] Table 4 Test groups

[0217] 4. Experimental Procedure

[0218] a) Pretreatment before the experiment: Thaw in physiological saline, cut Bama Xiang pig skin from the whole piece of pig skin using a special knife die (diameter 32.5 mm), and wash with physiological saline before use.

[0219] b) Sample loading:

[0220] ① Smearing group: First, fix the ex vivo skin into the diffusion cell, then add 68 μL of the material to the surface of the independent donor skin and spread it evenly by hand;

[0221] ② Atomizer group: First, fix the ex vivo skin into the diffusion cell, then load the sample through the atomizer, atomize for 5 seconds, and evenly spray it on the surface of the donor skin;

[0222] ③ Nebulizer + penetration enhancer group: First, atomize the sample onto the ex vivo skin surface (4cm*4cm) for 5 seconds, then slide the penetration enhancer in the anti-aging mode for 15 seconds at level 3, and then cut and fix it into the diffusion cell;

[0223] c) After sample loading, the pigskin is placed on the permeation cell and fixed with a silicone quantitative loop and an iron clamp between the diffusion chamber and the receiving chamber of the Franz diffusion unit, wherein the pigskin stratum corneum faces the diffusion chamber and the silicone quantitative loop is placed above the pigskin.

[0224] d) Fill the receiving chamber of the vertical diffusion cell with physiological saline to ensure close contact between the pigskin dermis and the receiving solution. Control the total volume of the receiving solution to 7 mL and place the accompanying magnetic stirring bar in the receiving chamber.

[0225] e) Place the Franz cell in an in vitro osmotic diffusion apparatus and preheat to 32°C. Set the magnetic stirring speed of the receiving cell to 200 rpm and ensure that there are no bubbles in the water bath.

[0226] f) After infiltration, remove the pigskin from the infiltration tank and wash the skin surface three times with physiological saline. Wipe the surface clean with lint-free paper, then excise the central region of the epidermis and store in 4% paraformaldehyde fixative. Section the tissue longitudinally, then image it under a fluorescence microscope.

[0227] g) Analyze the fluorescence signal using image analysis software and output fluorescence images, penetration depth, total skin fluorescence intensity, and dermal fluorescence intensity at each time point.

[0228] Experimental results:

[0229] 1) After 2 hours of transdermal absorption, the penetration depth of the smear group was 178 μm, the nebulizer group was 321 μm, and the nebulizer + permeation enhancer group was 464 μm. The nebulizer group experienced an 80.3% increase in percutaneous penetration compared to the smear group, while the nebulizer + permeation enhancer group experienced a 160.6% increase in percutaneous penetration compared to the smear group. For details, see Figure 7, which shows the skin penetration distribution of the active ingredient under different application methods.

[0230] 2) After 4 hours of transdermal absorption, the penetration depth of the smear group was 214 μm, the nebulizer group was 526 μm, and the nebulizer + permeation enhancer group was 642 μm. The nebulizer group experienced a 145.8% increase in percutaneous penetration compared to the smear group, while the nebulizer + permeation enhancer group experienced a 200% increase in percutaneous penetration compared to the smear group. See Figure 7 for details.

[0231] 3) After 2 hours of transdermal absorption, the fluorescence intensity of the entire skin layer was 1016182 in the smear group, 1327021 in the nebulizer group, and 1587213 in the nebulizer + permeation enhancer group. The fluorescence intensity of the entire skin layer increased by 30.6% in the nebulizer group compared to the smear group, and by 56.2% in the nebulizer + permeation enhancer group. For details, see Figure 8, which shows the fluorescence quantification data of active ingredients in the skin under different usage methods.

[0232] 4) After 4 hours of transdermal absorption, the fluorescence intensity of the entire skin layer was 1815665 in the smear group, 2059471 in the nebulizer group, and 2573081 in the nebulizer + permeation enhancer group. The fluorescence intensity of the entire skin layer increased by 13.4% in the nebulizer group compared to the smear group, and by 24.9% in the nebulizer + permeation enhancer group. See Figure 8 for details.

[0233] 5) After 4 hours of transdermal absorption, the fluorescence intensity of the dermis in the smear group was 38562.9, the fluorescence intensity of the dermis in the nebulizer group was 681855.6, and the fluorescence intensity of the dermis in the nebulizer + penetration-enhancing device group was 1665097.7. The fluorescence intensity of the dermis in the nebulizer group was 17.7 times that of the smear group, and the fluorescence intensity of the dermis in the nebulizer + penetration-enhancing device group was 43.2 times that of the smear group. For details, please refer to Figures 9 and 10. Figure 9 is a fluorescence distribution diagram of the active substance in the dermis under different usage methods, and Figure 10 is the fluorescence quantitative data of the active substance in the dermis under different usage methods.

[0234] Experimental studies were conducted on the components and amounts of the skin care actives provided herein. The differences between the examples are as follows: the components and amounts of the skin care actives, as shown in Table 5, are identical in the base material used in each example, specifically: 4 parts by weight of glycerin, 2 parts by weight of polydimethylsiloxane, 2 parts by weight of squalane, 0.6 parts by weight of acrylates, 1 part by weight of polysorbate 60, and the remaining parts by weight of deionized water, for a total of 100 parts by weight. Specifically, the five-fold hyaluronic acid in Examples 1-27 is composed of hydrolyzed sodium hyaluronate and sodium hyaluronate, with the weight ratio of hydrolyzed sodium hyaluronate to sodium hyaluronate being 3:2. The sodium hyaluronate includes sodium hyaluronate with a molecular weight of 220 WDa, sodium hyaluronate with a molecular weight of 138 WDa, and sodium hyaluronate with a molecular weight of 36 WDa. The hydrolyzed sodium hyaluronate includes hydrolyzed sodium hyaluronate with a molecular weight of 3.9 WDa and hydrolyzed sodium hyaluronate with a molecular weight of 7-8 kDa. The quadruple hyaluronic acid in Example 1-27 is composed of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate, and the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate is 0.4:1:1:0.1.

[0235] It can be seen from the above Examples 1 to 26 that the skin care active ingredients provided in the examples of the present application have moisturizing effects.

[0236] The above description is only an implementation method of the present application and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the description and drawings of this application, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. Use of a skin care active ingredient in the preparation of skin care products, wherein an atomizer and / or a penetration enhancer is used to promote the penetration of the skin care active ingredient into the skin; wherein the skin care active ingredient includes a humectant, and the humectant includes at least one of ceramide NP, sodium hyaluronate hydrolyzate, sodium hyaluronate, acetylated hyaluronic acid, sodium hyaluronate cross-linked polymer, and D-panthenol.

2. Use of the skin care active substance according to claim 1 in the preparation of skin care products, wherein, The skin care active ingredient further includes at least one of a moisturizing and firming agent, a soothing and anti-allergic agent, an antioxidant, a soother, and an anti-wrinkle and firming agent; the moisturizing and firming agent includes at least one of hydrolyzed lupetide protein and collagen XVII; the soothing and anti-allergic agent includes peony root extract; the anti-wrinkle and firming agent includes at least one of acetyl hexapeptide-8, tripeptide-1, acetyl octapeptide-3, snake venom-like peptide, palmitoyl tripeptide-5, palmitoyl tripeptide-1, palmitoyl hexapeptide-12, acetyl tetrapeptide-11, and hexapeptide-3; the antioxidant includes at least one of ergothioneine, coenzyme Q10, VC derivatives, VE derivatives, VC glucoside, tocopheryl acetate, silymarin, and dragon's blood extract; the soother includes at least one of purslane extract, dipotassium glycyrrhizinate, kava pepper extract, and trans-4-tert-butylcyclohexanol.

3. Use of the skin care active ingredient according to claim 2 in the preparation of skin care products, wherein the skin care active ingredient includes 3-90 parts by weight of the anti-wrinkle and firming agent, 0.1-15 parts by weight of the humectant, and 0.05-2.4 parts by weight of other components according to a weight ratio; wherein the anti-wrinkle and firming agent includes a first anti-wrinkle and firming agent and / or a second anti-wrinkle and firming agent, the first anti-wrinkle and firming agent includes at least one of acetyl hexapeptide-8, tripeptide-1, acetyl octapeptide-3, and snake venom-like peptide; the second anti-wrinkle and firming agent includes at least one of palmitoyl tripeptide-5, palmitoyl tripeptide-1, palmitoyl hexapeptide-12, acetyl tetrapeptide-11, and hexapeptide-3; the humectant includes at least one of ceramide NP, sodium hyaluronate hydrolyzate, and sodium hyaluronate; the other components include at least one of 0.05-0.4 parts by weight of the moisturizing and firming agent and 0.05-2 parts by weight of the soothing and anti-allergic agent; wherein the moisturizing and firming agent includes at least one of hydrolyzed lupetide protein and collagen XVII, and the soothing and anti-allergic agent includes peony root extract.

4. Use of the skin care active substance according to claim 3 in the preparation of skin care products, wherein, The skin care active ingredient is composed of the following components according to a weight ratio: 3-10 parts by weight of acetyl hexapeptide-8, 3-10 parts by weight of palmitoyl tripeptide-5, 0.05-0.2 parts by weight of hydrolyzed lupetide protein, 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of sodium hyaluronate hydrolyzate and sodium hyaluronate, and 0.05-2 parts by weight of peony root extract; wherein, in the 0.1-10 parts by weight of sodium hyaluronate hydrolyzate and sodium hyaluronate, the weight ratio of the sodium hyaluronate hydrolyzate to the sodium hyaluronate is (2.5-4):(1.5-3).

5. Use of the skin care active substance according to claim 2 in the preparation of skin care products, wherein, The skin care active substance comprises at least two of the following components in parts by weight: 0.0001-12 parts by weight of the antioxidant, 0.05-0.4 parts by weight of the moisturizing and firming agent, 0.05-2 parts by weight of the soothing and anti-allergic agent, and 0.1-15 parts by weight of the humectant; Among them, the antioxidant includes at least one of ergothioneine, coenzyme Q10, VC derivatives, and VE derivatives; the moisturizing and firming agent includes at least one of hydrolyzed lupetide protein and collagen XVII; the soothing and anti-allergic agent includes peony root extract; the humectant includes at least one of ceramide NP, hydrolyzed sodium hyaluronate, and sodium hyaluronate.

6. Use of the skin care active substance according to claim 5 in the preparation of skin care products, wherein, The skin care active substance consists of the following components in parts by weight: 0.0001-3 parts by weight of ergothioneine, 0.05-0.2 parts by weight of hydrolyzed lupetide protein, 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, and 0.05-2 parts by weight of peony root extract; Among them, in the 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, the weight ratio of hydrolyzed sodium hyaluronate to sodium hyaluronate is (2.5-4):(1.5-3).

7. Use of the skin care active substance according to claim 2 in the preparation of skin care products, wherein, The skin care active substance comprises 0.31-13 parts by weight of the antioxidant and 0.1-10 parts by weight of the humectant in parts by weight; Among them, the antioxidant includes 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, and 0.1-5 parts by weight of dragon's blood extract; the humectant includes at least one of sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, sodium hyaluronate, and acetylated sodium hyaluronate.

8. Use of the skin care active substance according to claim 7 in the preparation of skin care products, wherein, The skin care active substance consists of the following components in parts by weight: 0.1-5 parts by weight of VC glucoside, 0.1-2 parts by weight of tocopheryl acetate, 0.01-1 parts by weight of silymarin, 0.1-5 parts by weight of dragon's blood extract, 0.1-10 parts by weight of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate. The sodium hyaluronate cross-linked poly mer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate have a weight ratio of (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

9. Use of the skin care active substance according to claim 2 in the preparation of skin care products, wherein, The skin care active substance comprises 0.1-15 parts by weight of the humectant and 0.1-12 parts by weight of the soother in parts by weight; The moisturizing agent includes at least one of quadruple hyaluronic acid and D-panthenol, and the quadruple hyaluronic acid includes sodium hyaluronate cross-polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate; the soothing agent includes at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol.

10. Use of the skin care active ingredient according to claim 9 in the preparation of skin care products, wherein: The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, 0.1-1 parts of kava extract, and 0.1-5 parts by weight of unicorn extract; Wherein, the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate in the quadruple hyaluronic acid is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

11. Use of the skin care active ingredient according to claim 2 in the preparation of skin care products, wherein: The skin care active ingredients include 0.1-15 parts by weight of the moisturizer and 0.05-2 parts by weight of the soothing anti-allergic agent; The moisturizing agent includes at least two of ceramide NP, hydrolyzed sodium hyaluronate, and sodium hyaluronate; and the soothing and anti-allergic agent includes peony root extract.

12. Use of the skin care active ingredient according to claim 11 in preparing a skin care product, wherein: The skin care active ingredient is composed of the following components in the following weight ratios: 0.5-5 parts by weight of ceramide NP, 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, 0.05-2 parts by weight of peony root extract, and 0.01-5 parts by weight of acetyl glucosamine; Among them, in the 0.1-10 parts by weight of hydrolyzed sodium hyaluronate and sodium hyaluronate, the weight ratio of the hydrolyzed sodium hyaluronate to the sodium hyaluronate is (2.5-4):(1.5-3).

13. Use of the skin care active substance according to claim 1 in the preparation of skin care products, wherein, The permeation-promoting device includes at least one electrode group; The control method of the penetration-promoting device includes: Electrically stimulating the target site based on the electrode groups corresponding to the multiple penetration-enhancing modes; Among them, at least some of the working parameters between the penetration-enhancing modes are different; at least one of the penetration-enhancing modes corresponds to a first working parameter, the current output frequency range of the first working parameter is not less than 30kHz and not greater than 50kHz, and the corresponding peak-to-peak value range is 30Vpp to 56Vpp; at least one of the penetration-enhancing modes corresponds to a second working parameter, the current output frequency range of the second working parameter is greater than 100kHz and less than 400kHz, and the corresponding peak-to-peak value range is 60Vpp to 70Vpp.

14. Use of the skin care active substance according to claim 1 in the preparation of skin care products, wherein, The atomizer applies the skin care active ingredient to the skin surface in the form of a spray; The atomized particles generated by the spraying method have a D50 particle size of 10 μm to 50 μm.

15. Use of the skin care active substance according to claim 14 in the preparation of skin care products, wherein, The atomized particles generated by the spraying method have a D50 particle size of 10 μm to 30 μm.

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