Fluid conveying system and method, and gene sequencer

Abstract

A fluid conveying system and method, and a gene sequencer. The fluid conveying system comprises a fluid storage module, a first working module, and a second working module. The fluid storage module is used for storing a plurality of fluids. The first working module comprises a fluid selection assembly and a first power assembly, and the second working module comprises a second power assembly. At least one of the first working module and the second working module is selectively communicated with a fluid utilization system. The fluid selection assembly is used for selecting one fluid from the fluid storage module, and the power assembly is used for driving the selected fluid to be transferred to the fluid utilization system. The second power assembly is used for driving another fluid in the fluid storage module to be transferred to the fluid utilization system.

Description

Fluid transport system, method and gene sequencer Technical Field

[0001] The present application relates to fluid control, and in particular to a fluid transport system, method and gene sequencer. Background Art

[0002] In fields such as biology, chemistry, and medicine, common instruments are designed based on the core principles of biological or chemical reactions. Substances involved in biological or chemical reactions (such as reagents) are typically liquid or gaseous in physics and are collectively referred to as fluids. To perform the desired biological or chemical reactions, the instrument typically requires containers that serve as reaction pools for samples and fluids, and a transport system is also required to quantitatively deliver the different fluids to the reaction pools.

[0003] In related art, the transport system includes a manifold plate that cooperates with the reaction tank. The upstream end of the manifold plate is also equipped with a reversing component (such as a rotary valve, solenoid valve, etc.) for switching the direction of the liquid path. However, due to the size of the component itself, the common pipeline between the reversing component and the manifold plate has a certain length. Therefore, when transporting different reagents to the manifold plate, the amount of reagent needs to be increased to replace the previous reagent remaining in this section of the common pipeline to avoid cross contamination. However, this undoubtedly results in additional reagent waste, leading to increased costs.

[0004] Summary of the Invention

[0005] In view of this, it is necessary to provide a fluid transport system, a fluid transport method and a gene sequencer.

[0006] In a first aspect, the present application provides a fluid transport system for transporting fluid to a fluid-using system. The fluid transport system includes a fluid storage module, a first working module, and a second working module. The fluid storage module is used to store multiple fluids. The first working module includes a fluid selection component and a first power component, and the second working module includes a second power component. At least one of the first working module and the second working module is selectively connected to the fluid-using system. The fluid selection component is used to select a fluid from the fluid storage module, and the power component is used to drive the selected fluid to be transferred to the fluid-using system. The second power component is used to drive another fluid in the fluid storage module to be transferred to the fluid-using system.

[0007] A second aspect of the present application provides a fluid transport method for transporting fluid to a fluid-using system. The fluid transport method comprises: establishing a connection between a fluid storage module and a first working module and a second working module, wherein the first working module comprises a fluid selection component and a first power component, and the second working module comprises a second power component; the first power component is activated, thereby selecting a fluid from the fluid storage module through the fluid selection component and driving the selected fluid to be transferred to the fluid-using system; and the second power component is activated, thereby driving another fluid in the fluid storage module to be transferred to the fluid-using system.

[0008] The third aspect of the present application provides a gene sequencer, comprising the above fluid transport system or used to execute the above fluid transport method.

[0009] The fluid transport system of the present application includes a first working module and a second working module. The second working module has a simpler structure and lower cost than the first working module. Different types of fluids can be transported to the fluid use system via the first working module and the second working module respectively. For example, most reagents can be transported to the fluid use system via the first working module, while a single reagent with a larger usage amount can be transported to the fluid use system via the second working module. This avoids fluid waste caused by different fluids sharing the common pipeline between the fluid selection component and the distribution module of the first working module. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] FIG1 is a module architecture diagram of a fluid transport system provided in one embodiment of the present application.

[0011] FIG2 is a module architecture diagram of the fluid transportation system shown in FIG1 when operating the first working logic.

[0012] FIG3 is a module architecture diagram of the fluid transportation system shown in FIG1 when operating the second working logic.

[0013] FIG4 is a module architecture diagram of the fluid transportation system shown in FIG1 when operating the third working logic.

[0014] FIG5 is a module architecture diagram of the fluid transportation system shown in FIG1 when operating the fourth working logic.

[0015] FIG6 is a schematic structural diagram of the fluid transport system provided in Example 1.

[0016] 7 to 14 are schematic diagrams showing the flow of fluid when the fluid transport system shown in FIG. 6 performs different functions.

[0017] FIG15 is a schematic structural diagram of the fluid transport system provided in Example 2.

[0018] 16 to 18 are schematic diagrams showing the flow of fluid when the fluid transport system shown in FIG. 15 performs different functions.

[0019] FIG19 is a schematic structural diagram of the fluid transport system provided in Example 3.

[0020] 20 to 25 are schematic diagrams showing the flow of fluid when the fluid transport system shown in FIG. 19 performs different functions.

[0021] FIG26 is a flow chart of a fluid transport method provided in one embodiment of the present application.

[0022] Figure 27 is a module architecture diagram of a gene sequencer provided in one embodiment of the present application.

[0023] The following specific implementation methods will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present application in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments.

[0025] It should be noted that when a component is referred to as being "fixed to" or "mounted on" another component, it may be directly on the other component or there may be a central component. When a component is referred to as being "disposed on" another component, it may be directly on the other component or there may be a central component. As used herein, the term "and / or" includes all and any combinations of one or more of the relevant listed items.

[0026] It should be noted that, although a logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in an order different from that in the flowchart. The method disclosed in the embodiments of the present application includes one or more steps or actions for implementing the method. The method steps and / or actions may be interchangeable with each other without departing from the scope of the claims. Unless a specific order of steps or actions is specified, the order and / or use of specific steps and / or actions may be modified without departing from the scope of the claims.

[0027] 1 , an embodiment of the present application provides a fluid transport system 100 for transporting fluid to a fluid using system 200. The fluid using system 200 is a system that uses fluid and is the destination of the fluid transport system 100 transporting the fluid.

[0028] The fluid transport system 100 includes a fluid storage module 10, at least two working modules 20, a distribution module 30, and a waste module 40. The fluid storage module 10 is used to store fluids to be transported to the fluid use system 200. The fluid storage module 10 may include multiple independent fluid storage components, each of which is used to store a type of fluid. The fluids may include reagents involved in biochemical reactions, buffers, or cleaning fluids. The fluids stored in the multiple fluid storage components may be different. The fluid storage components may be containers suitable for storing and containing fluids, such as reagent tanks or test kits.

[0029] The working modules 20 are functional units within the fluid transport system 100 that drive fluid transport. Each working module 20 operates independently. When one working module 20 is operating, the remaining working modules 20 may not be operating. When at least two working modules 20 are operating, the at least two working modules 20 may operate in parallel or sequentially. "Operating in parallel" means that the operating times of the at least two working modules 20 completely overlap or at least partially overlap on the timeline, thereby optimizing the total time for fluid transport. Each working module 20 can be connected to the fluid storage module 10 and the distribution module 30. The distribution module 30 can also be connected to at least one of the fluid use system 200 and the waste module 40. The distribution module 30 is used to distribute the fluid transported by the working modules 20 to the fluid use system 200 or the waste module 40. After the fluid enters the fluid use system 200 and is used for a certain purpose within the fluid use system 200, it is discharged as waste liquid to the waste module 40. As used herein, "connected" or "connected" means that the modules are connected or communicated with each other via piping or other suitable components. There may be multiple connection paths between the dispensing module 30 and the fluid using system 200 , each connection path being used for transporting fluid between the dispensing module 30 and the fluid using system 200 .

[0030] The working module 20 can be divided into a first working module 21 and a second working module 22 according to its components. The fluid transport system 100 may include at least one first working module 21 and at least one second working module 22. The first working module 21 includes a first power component 211 and a fluid selection component 212. The fluid selection component 212 can be connected to the first power component 211 and at least part of the fluid storage element of the fluid storage module 10 (hereinafter referred to as the first fluid storage element 11, which can be used to store reagents, buffers or cleaning fluids). The fluid selection component 212 can also be connected to the first power component 211 and the distribution module 30. The first power component 211 is used to create and maintain a pressure gradient (pressure difference) within the fluid transport system 100, thereby driving the fluid to move in the fluid transport system 100. The first power assembly 211 can drive the movement of fluid in both positive and negative directions. The positive direction refers to the direction of pushing the fluid so that it flows from the first power assembly 211 to the fluid selection assembly 212 and the distribution module 30 in sequence. The reverse direction refers to the direction of extracting the fluid so that it flows from the first fluid storage element 11 to the fluid selection assembly 212 and the first power assembly 211 in sequence. The first working module 21 may further include a fluid transfer assembly 213. The fluid transfer assembly 213 may be connected between the first power assembly 211 and the fluid selection assembly 212. The fluid transfer assembly 213 may be a container or pipeline connected between the first power assembly 211 and the fluid selection assembly 212, and is used to temporarily store fluid that is transported from the first fluid storage element 11 in the reverse direction and needs to change the transport direction. In other embodiments, the fluid transfer assembly 213 may also be a cavity of the first power assembly 211 itself. Among them, a negative pressure drive method is used to extract the fluid and temporarily store it in the fluid transfer component 213, which is beneficial to improving the quantitative accuracy of the extracted fluid; a positive pressure drive method is used to input the temporarily stored fluid into the distribution module 30, which is beneficial to quickly transport the fluid to the distribution module 30 and improve the fluid transportation efficiency.

[0031] The first power assembly 211 can also be connected to some fluid storage elements of the fluid storage module 10 (hereinafter referred to as the second fluid storage element 12, which can be used to store reagents, buffers, or cleaning fluids). Because the first power assembly 211 is directly connected to the second fluid storage element 12, the first power assembly 211 can also drive the fluid in the second fluid storage element 12 to flow out in the positive direction and temporarily store it in the fluid transfer assembly 213. By further providing the second fluid storage element 12, the types of fluids stored in the fluid storage module 10 can be increased. In some embodiments, the first power assembly 211 can be various types of pumps for driving fluid movement, such as syringe pumps, plunger pumps, diaphragm pumps, gear pumps, and peristaltic pumps.

[0032] The fluid selection assembly 212 is used to interconnect the various components connected to the fluid selection assembly 212, thereby enabling the selection of a path for transporting fluids. For example, when fluid within a first fluid storage element 11 of the fluid storage module 10 needs to be transported to the fluid transfer assembly 213 for temporary storage, the fluid selection assembly 212 can selectively connect the first power assembly 211 to a first fluid storage element 11. Alternatively, when fluid temporarily stored in the fluid transfer assembly 213 needs to be transported to the distribution module 30, the fluid selection assembly 212 can connect the first power assembly 211 to the distribution module 30. It will be appreciated that if the fluid transported to the distribution module 30 is a reagent, the reagent can be further transported to the fluid utilization system 200 connected to the distribution module 30, allowing the reagent to undergo a biochemical reaction within the fluid utilization system 200. If the fluid transported to the distribution module 30 is a cleaning fluid, the reagent can be further transported to the fluid utilization system 200 and the waste fluid module 40, allowing the cleaning fluid to clean the internal flow channels of the components it flows through or the connecting pipes between components. The fluid selection assembly 212 can be a valve such as a solenoid valve, a selection valve (such as a rotary valve), or a combination thereof. The fluid selection assembly 212 can also further include at least one liquid extraction needle 2121 (such as a reagent needle, shown in FIG15 ) connected to the valve of the fluid selection assembly 212 , each liquid extraction needle 2121 being used to extend into the fluid storage module 10 to extract the desired fluid. In some embodiments, there is only one liquid extraction needle 2121, which can be mechanically moved to a corresponding position in the fluid storage module 10 to extract the desired fluid. For example, the liquid extraction needle 2121 can perform multi-axis movement to extend into different fluid storage elements in the fluid storage module 10 to extract the desired fluid, thereby reducing the number of liquid extraction needles 2121, which helps reduce hardware costs and save space in the fluid transport system 100. In other embodiments, there can be multiple liquid extraction needles 2121, and the multiple liquid extraction needles 2121 can correspond to the multiple first fluid storage elements 11 of the fluid storage module 10.

[0033] The second working module 22 includes a second power assembly 221 but does not include a fluid selection assembly. Therefore, the second power module has a simpler structure and lower cost than the first working module 21. The second power assembly 221 of the second working module 22 can be connected to a part of the fluid storage element (hereinafter referred to as the third fluid storage element 13, which can be used to store reagents) and the distribution module 30 of the fluid storage module 10. The second power assembly 221 can drive the fluid in the third fluid storage element 13 in the positive direction to flow out and be temporarily stored (such as temporarily stored in the cavity of the second power assembly 221), and can also drive the temporarily stored fluid in the positive direction to enter the distribution module 30. In some embodiments, the second power assembly 221 can be various types of pumps for driving fluid movement, for example, a syringe pump, a plunger pump, a diaphragm pump, a gear pump, and a peristaltic pump.

[0034] The distribution module 30 is used to connect the different components connected to the distribution module 30 to each other, so as to realize the path selection for transporting the fluid. For example, when it is necessary to transport the fluid temporarily stored in the fluid transfer component 213 to the fluid use system 200, the distribution module 30 can connect the fluid selection component 212 with the fluid use system 200. Alternatively, when it is necessary to transport the fluid temporarily stored in the fluid transfer component 213 directly to the waste liquid module 40, the distribution module 30 can connect the fluid selection module with the waste liquid module 40. Among them, the waste liquid module 40 can be a container suitable for storing and accommodating waste liquid. The distribution module 30 can be a reversing component or a fluid storage tank / flow channel. The reversing component can be various types of solenoid valves, selection valves or combinations thereof. The fluid storage tank / flow channel can be a manifold block or a hose.

[0035] In some embodiments, the fluid transport system 100 may further include a cleaning and emptying module 50, which is used to clean other modules of the fluid transport system 100. The cleaning and emptying module 50 includes at least one third power assembly 51. The third power assembly 51 can be connected to at least part of the fluid storage elements of the fluid storage module 10 (such as the first fluid storage element 11 for storing cleaning fluid), and can also be connected to other modules in the fluid transport system 100 that need to be cleaned, as well as the waste liquid module 40. For example, the third power assembly 51 can be connected to at least one of the first working module 21, the second working module 22, and the distribution module 30. The third power assembly 51 is used to drive the cleaning fluid stored in the first fluid storage element 11 to flow out, then flow through the module in the fluid transport system 100 that needs to be cleaned to clean the module, and the waste liquid after cleaning enters the waste liquid module 40 for storage.

[0036] Furthermore, the cleaning and emptying module 50 can also be used to empty residual fluids in other modules of the fluid transport system 100. For example, when the first fluid storage element 11 of the fluid storage module 10 needs to be emptied, the third power assembly 51 can be connected to the fluid selection assembly 212 of the first working module 21 and the waste module 40. The fluid selection assembly 212 can also be connected to the first fluid storage element 11. In this way, the third power assembly 51 can drive the fluid (e.g., reagent) in the first fluid storage element 11 to flow out in the opposite direction and enter the waste module 40 through the fluid selection assembly 212 and the third power assembly 51, thereby emptying the remaining fluid in the first fluid storage element 11. The third power assembly 51 can be a power source or a reversing component. The power source can be a syringe pump, a plunger pump, a diaphragm pump, a gear pump, a peristaltic pump, etc. The reversing component can be various types of solenoid valves, selection valves, pinch valves, or combinations thereof. Compared with using the first power component 211 or the second power component 221 to drive the cleaning liquid to clean or empty each module, some embodiments of the present application further introduce a cleaning and emptying module 50 to clean each module. The cleaning and emptying module 50 can work in parallel with the first working module 21 or the second working module 22 to improve the cleaning or emptying efficiency, and is beneficial to extending the service life of the first power component 211 or the second power component 221.

[0037] In some embodiments, the fluid transport system 100 may further include a motion control module 60. When the fluid selection assembly 212 includes a liquid extraction needle 2121 for extracting fluid, the motion control module 60 is used to control the movement of the liquid extraction needle 2121 and align it with different fluid storage elements, thereby enabling the transport of different types of fluids. The motion control module 60 can be connected to the fluid storage module 10. When the same fluid storage element of the fluid storage module 10 contains at least two fluids, the motion control module 60 is further used to control the movement of the fluid storage module 10 so that the fluids stored in the fluid storage element are evenly mixed. The motion control module 60 can be a multi-axis motion platform or a rotating platform capable of mechanical movement.

[0038] The following will describe the three working logics of the fluid transportation system 100 in combination with various components of the fluid transportation system 100 .

[0039] The first working logic

[0040] Under the first operating logic, only the first operating module 21 operates. Figure 2 illustrates the operation of the first operating module 21 as an example. Before fluid transport, assume that the fluid utilization system 200 is filled with buffer, and that the distribution module 30, the fluid selection module, and the first power assembly 211 are filled with air. The specific process of fluid transport by the fluid transport system 100 can be broken down into five steps: Steps 1 through 5.

[0041] In step 1, the first power assembly 211 connects the fluid storage module 10 (e.g., the first fluid storage element 11 or the second fluid storage element 12 of the fluid storage module 10) and the fluid selection assembly 212. Simultaneously, the distribution module 30 connects the fluid selection assembly 212 and the waste module 40, thereby forming a pathway that begins at the fluid storage module 10 and passes through the first power assembly 211, the fluid selection assembly 212, the distribution module 30, and the waste module 40. The first power assembly 211 is then activated to create a pressure gradient within this pathway, causing fluid to flow from the fluid storage module 10, pass through the fluid selection assembly 212 and the distribution module 30, and finally enter the waste module 40 as waste for storage. In this step, the first power assembly 211 can be driven by negative pressure to transport fluid from the fluid storage module 10 to the waste module 40. This step is intended to fill the relevant pipelines (pre-priming) to ensure the accuracy of subsequent steps.

[0042] In step 2, the first power assembly 211 is connected to the fluid storage module 10 and the fluid selection assembly 212, and the fluid selection assembly 212 is also connected to the fluid storage module 10, thereby forming a passage starting from the fluid storage module 10 and passing through the first power assembly 211, the fluid selection assembly 212 and the fluid storage module 10 in sequence. Afterwards, the first power assembly 211 is started to create a pressure gradient in the passage, causing the fluid to flow out of the fluid storage module 10 and enter the fluid transfer assembly 213 for temporary storage. In this step, the first power assembly 211 can transport the fluid from the fluid storage module 10 to the fluid transfer assembly 213 by negative pressure drive. This step can be used to extract the target fluid (such as reagent 1).

[0043] In step three, the first power assembly 211 connects the fluid storage module 10 and the fluid selection assembly 212, while the distribution module 30 connects the fluid selection assembly 212 and the waste module 40, thereby forming a pathway that begins with the first power assembly 211 and passes through the fluid selection assembly 212, the distribution module 30, and the waste module 40. The first power assembly 211 is then activated to create a pressure gradient in this pathway, causing the fluid to flow from the fluid transfer assembly 213, pass through the fluid selection assembly 212 and the distribution module 30, and finally enter the waste module 40 as waste for storage. In this step, the first power assembly 211 can transport the fluid from the fluid storage module 10 to the waste module 40 via positive pressure. This step is intended to discharge air and a portion of the lower-purity target fluid temporarily stored in the fluid transfer assembly 213 to the waste module 40, preparing for the subsequent transport of the higher-purity target fluid to the fluid utilization system 200 and ensuring the accuracy of subsequent steps.

[0044] In step four, the fluid selection assembly 212 connects the first power assembly 211 and the distribution module 30. The distribution module 30 also connects the fluid utilization system 200 and the waste module 40, thereby forming a pathway that begins with the first power assembly 211 and passes through the fluid selection assembly 212, the distribution module 30, the fluid utilization system 200, and the waste module 40. The first power assembly 211 is then activated to create a pressure gradient in this pathway, causing the fluid to flow from the fluid transfer assembly 213, pass through the fluid selection assembly 212, the distribution module 30, and the fluid utilization system 200, and finally enter the waste module 40 as waste for storage. In this step, the first power assembly 211 can transport the fluid from the fluid storage module 10 to the waste module 40 via positive pressure. This step is intended to transport the target fluid of higher purity to the fluid utilization system 200 for biochemical reactions. In other embodiments, step three may be omitted, that is, after executing step two to allow the fluid to flow out of the fluid storage module 10 and enter the fluid transfer component 213 for temporary storage, step four may be directly executed to transport the target fluid temporarily stored in the fluid transfer component 213 to the fluid use system 200.

[0045] In step five, the fluid selection assembly 212 connects to the first power assembly 211 and the distribution module 30, while the distribution module 30 also connects to the waste module 40, thereby forming a passage starting from the first power assembly 211 and sequentially passing through the fluid selection assembly 212, the distribution module 30, and the waste module 40. The first power assembly 211 is then activated to create a pressure gradient in this passage, causing the remaining air and fluid to flow out of the fluid transfer assembly 213, pass through the fluid selection assembly 212 and the distribution module 30, and finally enter the waste module 40 as waste liquid for storage. In this step, the first power assembly 211 can transport fluid from the fluid storage module 10 to the waste module 40 by means of positive pressure drive. This step is intended to discharge the air and target fluid remaining in the fluid transfer assembly 213 to the waste module 40.

[0046] After the target fluid is transported to the fluid use system 200, other types of target fluids (such as reagent 2, reagent 3, etc.) can be further transported to the fluid use system 200, so that different fluids undergo biochemical reactions in the fluid use system 200. Specifically, steps 2 to 5 can be repeated to transport one target fluid to the fluid use system 200 each time. The fluid selection component 212 can be operated to selectively connect the fluid selection component 212 to different fluid storage components in the fluid storage module 10, thereby achieving the transportation of different types of fluids. Alternatively, the motion control module 60 can be connected to the fluid storage module 10 and drive the fluid storage module 10 to move, so that the fluid selection component 212 connects to different fluid storage components in the fluid storage module 10. In the first working logic, each step and its purpose are recorded in Table 1.

[0047] Table 1, the operation process of the first working logic

[0048] The second working logic

[0049] Under the second operating logic, only the second operating module 22 operates. FIG3 illustrates the operation of a second operating module 22 as an example. The second operating module 22 omits the fluid selection component 212, and this operating logic is suitable for transporting a single, large-volume fluid to the fluid use system 200. Prior to fluid transport, it is assumed that the fluid use system 200 is filled with buffer, and the distribution module 30 and the second power assembly 221 are filled with air. The specific process of transporting fluid by the fluid transport system 100 can be broken down into two steps: step one and step two.

[0050] In step 1, the second power assembly 221 connects the fluid storage module 10 (e.g., the third fluid storage element 13 of the fluid storage module 10, used to store reagents) and the distribution module 30. The distribution module 30 is also connected to the waste module 40, thereby forming a pathway that begins at the fluid storage module 10 and passes through the second power assembly 221, the distribution module 30, and the waste module 40. The first power assembly 211 is then activated to create a pressure gradient in this pathway, causing the fluid to flow from the fluid storage module 10, pass through the distribution module 30, and finally enter the waste module 40 as waste for storage. In this step, the second power assembly 221 can transport the fluid from the fluid storage module 10 to the waste module 40 via negative pressure. This step is intended to fill the relevant pipelines to ensure the accuracy of subsequent steps.

[0051] In step 2, the second power assembly 221 connects the fluid storage module 10 and the distribution module 30. The distribution module 30 also connects the fluid use system 200 and the waste module 40, thereby forming a pathway that begins at the fluid storage module 10 and passes through the second power assembly 221, the distribution module 30, the fluid use system 200, and the waste module 40. The second power assembly 221 is then activated to create a pressure gradient in this pathway, causing fluid to flow from the fluid storage module 10, through the distribution module 30 and the fluid use system 200, thereby replacing the buffer in the fluid use system 200 with the target fluid. Finally, the buffer and excess target fluid enter the waste module 40 as waste for storage. In this step, the first power assembly 211 can transport the fluid from the fluid storage module 10 to the waste module 40 via negative pressure. This step is intended to transport the target fluid to the fluid use system 200 for biochemical reactions.

[0052] In the second working logic, each step and its purpose are recorded in Table 2.

[0053] Table 2, the operation process of the second working logic

[0054] The third working logic

[0055] Under the third working logic, the first working module 21 and the second working module 22 work in parallel. FIG4 is taken as an example to illustrate a first working module 21 and a second working module 22. The first working module 21 and the second working module 22 respectively perform their corresponding functions. Specifically, the functions performed by the first working module 21 can refer to the execution steps of the above-mentioned first working logic, and the functions performed by the second working module 22 can refer to the execution steps of the above-mentioned second working logic. In this embodiment, the first working module 21 and the second working module 22 work in parallel, thereby shortening the total time for transporting the fluid. Before the fluid is transported, it is assumed that the fluid use system 200 is filled with buffer solution, and the distribution module 30, the fluid selection component 212, the first power component 211 and the second power component 221 are filled with air.

[0056] In the third kind of working logic, each step and purpose performed by the first working module 21 and the second working module 22 are recorded in Table 3. In Table 3, the steps located in the same row are parallel steps. For example, the pre-filling step performed by the first working module 21 is performed in parallel with the pre-filling step performed by the second working module 22, and they at least partially overlap on the time axis. For another example, when a certain fluid is carrying out a biochemical reaction in the fluid use system 200, the first working module 21 or the second working module 22 can pre-pump the next fluid, thereby saving time. Wherein, Table 3 is only a schematic illustration. In actual applications, the type of fluid and the amount of fluid required to be transported can also be changed according to actual needs. In other embodiments, based on different needs, the first working module 21 and the second working module 22 can also work in series.

[0057] Table 3, the operation process of the third working logic

[0058] As shown in the operating process of Table 3, when a certain fluid undergoes a biochemical reaction in the fluid use system 200, the first power component 211 can drive the next reagent from the fluid storage module 10 to the fluid transfer component 213 for temporary storage (i.e., the pre-pumping step). If the previous biochemical reaction is still in progress after the pre-pumping, it is necessary to wait until the biochemical reaction is completed before transporting the pre-pumped fluid to the fluid use system 200 for the next biochemical reaction.

[0059] In other embodiments, if the previous biochemical reaction is still ongoing after the pre-pump, the first power assembly 211 may also drive the fluid temporarily stored in the fluid transfer assembly 213 to be transported to the second working module 22 for temporary storage, thereby freeing up the first working module 21 so that the first working module 21 can pre-pump the next fluid to the fluid transfer assembly 213. In this embodiment, each step performed by the first working module 21 and the second working module 22 and its purpose are recorded in Table 4.

[0060] Table 4, another operation process of the third working logic

[0061] It can be seen from the operation process of Table 4 that if the previous biochemical reaction is still in progress after pre-pumping reagent 2, the first power component 211 drives the reagent 2 temporarily stored in the fluid transfer component 213 to be transported to the second working module 22 for temporary storage, thereby releasing the first working module 21 so that the first power component 211 can pre-pump reagent 3 in advance, that is, avoiding the problem that the first power component 211 needs to wait for the previous biochemical reaction to be completed and the temporarily stored reagent 2 to be transported to the fluid use system 200 before pre-pumping reagent 3, thereby making full use of the time of the biochemical reaction to pre-pump the next fluid, saving fluid transportation time.

[0062] The fourth working logic

[0063] The cleaning and emptying module 50 operates under the fourth operating logic. The third power assembly 51 of the cleaning and emptying module 50 can be connected to at least some of the fluid storage elements of the fluid storage module 10 (such as the first fluid storage element 11 for storing cleaning fluid), and can also be connected to other modules in the fluid transport system 100 that need to be cleaned, as well as the waste fluid module 40. Figure 5 uses the example of the cleaning and emptying module 50 cleaning the first working module 21 and the distribution module 30. Before fluid transport, assuming there is residual fluid (such as residual reagents after a biochemical reaction) in the first working module 21 and the distribution module 30, the cleaning steps of the cleaning and emptying module 50 are as follows:

[0064] The third power assembly 51 of the cleaning and emptying module 50 is connected to the fluid storage module 10 and the first power assembly 211. The first power assembly 211 is further connected to the fluid selection assembly 212, which is further connected to the distribution module 30, which is further connected to the waste module 40. The third power assembly 51 is then activated to create a pressure gradient in the passage, causing fluid (such as cleaning fluid) to flow out of the fluid storage module 10, and then cleaning each module in the entire passage. The waste fluid after cleaning enters the waste module 40 for storage.

[0065] It is understood that the specific connection method between the third power assembly 51 and other modules of the fluid transport system 100 can be flexibly changed, and it is sufficient to connect the module to be cleaned to the flow path of the cleaning fluid driven by the third power assembly 51. When it is necessary to clean other modules of the fluid transport system 100, it is sufficient to connect the other modules to the flow path of the cleaning fluid driven by the third power assembly 51.

[0066] The emptying step of the cleaning and emptying module 50 is used to empty the residual fluid in other modules of the fluid transport system 100. Taking the cleaning and emptying module 50 to empty the first fluid storage element 11 of the fluid storage module 10 as an example, the emptying steps of the cleaning and emptying module 50 are as follows:

[0067] The third power assembly 51 of the cleaning and emptying module 50 is connected to the fluid storage module 10 and the first power assembly 211. The first power assembly 211 is also connected to the fluid selection assembly 212. The fluid selection assembly 212 is also connected to the distribution module 30. The distribution module 30 is also connected to the waste liquid module 40. The third power assembly 51 is then activated to create a pressure gradient in the passage, causing the fluid (such as non-cleaning fluid) to flow out of the fluid storage module 10 and into the waste liquid module 40 for storage, thereby emptying the non-cleaning fluid from the fluid storage module 10.

[0068] It can be understood that the specific connection method between the third power component 51 and other modules of the fluid transportation system 100 can be flexibly changed. When other modules of the fluid transportation system 100 need to be emptied, it is only necessary to connect the other modules that need to be emptied to the flow path of the third power component 51.

[0069] The fluid transport system 100 of the present application includes a first working module 21 and a second working module 22. The second working module 22 has a simpler structure and lower cost than the first working module 21. Different types of fluids can be transported to the distribution module 30 via the first working module 21 and the second working module 22, respectively, that is, different types of fluids have different paths for entering the distribution module 30. For example, most reagents can be transported to the distribution module 30 via the first working module 21, while a single reagent with a larger amount can be transported via the second working module 22, thereby eliminating fluid waste caused by different fluids sharing the common pipeline between the fluid selection component 212 and the distribution module 30 (when transporting different reagents, it is necessary to increase the amount of reagent to replace the previous reagent remaining in the common pipeline, thereby causing fluid waste).

[0070] Referring to FIG. 6 , in some specific embodiments, the fluid selection assembly 212 of the first working module 21 utilizes a rotary valve 2120, the first power assembly 211 utilizes a syringe pump 2110, and the fluid transfer assembly 213 is a fluid transfer line 2130 connected between the rotary valve 2120 and the syringe pump 2110. The second power assembly 221 of the second working module 22 utilizes a lower-cost plunger pump, peristaltic pump, or diaphragm pump, thereby further reducing the cost of the second working module 22. In this embodiment, the second power assembly 221 utilizes a plunger pump 2210.

[0071] The first fluid storage element 11, the second fluid storage element 12, and the third fluid storage element 13 of the fluid storage module 10 can each be a reagent kit. The number of reagent kits can also be changed according to actual needs. The waste liquid module 40 uses a waste liquid barrel 41.

[0072] The fluid utilization system 200 adopts flow cell slide C. The flow cell slide C has a flow channel (not shown) inside. The flow cell slide C is provided with a flow channel (not shown). The flow cell slide C is also provided with at least two openings 201 (three openings 201 are shown in the figure), and the fluid can flow into or out of the flow cell slide C through each opening 201. Wherein, at least one opening 201 can be used as an inlet for the fluid to flow in, and can also be used as an outlet for the fluid to flow out. Therefore, the inlet and outlet of the fluid loaded into the flow cell slide C are variable, and the fluid loading direction and loading path are variable. The mode of the fluid flowing through the flow cell slide C can be single in and single out, single in and multiple out, multiple in and single out, or multiple in and multiple out. For example, a fluid can flow into the flow cell slide C through one of the three openings 201 and then flow out through another opening 201, thereby achieving single inlet and single outlet; a fluid can also flow into the flow cell slide C through one of the three openings 201 and then flow out through the remaining two openings 201, thereby achieving single inlet and multiple outlets; a fluid can also flow into the flow cell slide C through two of the three openings 201 and then flow out through the remaining opening 201, thereby achieving multiple inlets and single outlets. Since the fluid loading direction and loading path are variable, cross contamination between different reagents can be avoided.

[0073] The distribution module 30 utilizes a first manifold block 31. A flow channel 310 is defined within the first manifold block 31. The first manifold block 31 is provided with at least two first fluid inlets 311 and one first fluid outlet 313, both of which are connected to the flow channel 310. FIG6 shows two first fluid inlets 311. One first fluid inlet 311 is connected to the fluid selection assembly 212 of the first working module 21 via a first input line P1, and the other first fluid inlet 311 is connected to the second power assembly 221 of the second working module 22 via a second input line P2. The first manifold block 31 is also provided with a plurality of slide interfaces (not shown), which are connected to each first fluid inlet 311 via a first valve 314. When the flow cell slide C is mounted on the first manifold block 31, the plurality of slide interfaces of the first manifold block 31 are respectively configured to correspond to the plurality of openings 201 of the flow cell slide C. The specific connection method between the first manifold block 31 and the flow cell slide C can be referred to in the international application number PCT / CN2023 / 138270 filed by the same applicant with a filing date of December 12, 2023, and will not be described in detail here. The flow channel 310 is also provided with a second valve 315, which is used to control the connection or disconnection of the flow channel 310 itself, thereby guiding the flow direction of the fluid. Thus, when the second valve 315 is open and the first valve 314 is closed, the fluid from the fluid selection assembly 212 can enter the flow channel 310 inside the first manifold block 31 through the first fluid inlet 311 and then flow out through the first fluid outlet 313. When one of the first valves 314 is open, the fluid from the fluid selection assembly 212 can also enter the flow channel 310 inside the first manifold block 31 through the first fluid inlet 311, then pass through the opened first valve 314 and the corresponding slide interface to enter the opening 201 of the flow cell slide C, then flow out through the other openings 201 of the flow cell slide C to the flow channel 310 of the first manifold block 31, and then flow out through the first fluid outlet 313. The first valve 314 and the second valve 315 can be solenoid valves or other types of valves.

[0074] A second fluid inlet 312 may also be provided on the first manifold block 31, and no valve is provided on the fluid path between the second fluid inlet 312 and the corresponding slide inlet. The fluid selection component 212 of the first working module 21 is also connected to the second fluid inlet 312 via the third input pipeline P3. The first input pipeline P1 and the third input pipeline P3 are independent of each other, so that the first fluid inlet 311 and the second fluid inlet 312 are respectively used to allow different types of fluids to flow in. Moreover. During actual operation, key sensitive reagents or reagents with smaller usage amounts can be selected and guided into the flow cell slide C using the second fluid inlet 312 of the first manifold block 31, thereby avoiding damage to the reagent by the first valve 314.

[0075] The functions of the fluid transport system 100 of the present application will be further described below based on the specific structure of the fluid transport system 100 and in combination with specific embodiments. Those skilled in the art should understand that the structures described in this application are only embodiments, and any other suitable structures are within the scope of this application.

[0076] Example 1

[0077] Referring to Figure 6 , Example 1 employs the third operating logic for fluid transport, with a first operating module 21 and a second operating module 22 operating in parallel. To simplify the diagram, inoperative modules in Example 1 have been omitted from Figure 6 . The fluid selection assembly 212 of the first operating module 21 utilizes a rotary valve 2120, the first power assembly 211 utilizes a syringe pump 2110, and the fluid transfer assembly 213 comprises a fluid transfer line 2130 connected between the rotary valve 2120 and the syringe pump 2110. The second power assembly 221 of the second operating module 22 utilizes a plunger pump 2210. The fluid storage module 10 includes three first fluid storage elements 11, one second fluid storage element 12, and one third fluid storage element 13. The three first fluid storage elements 11 are reagent kits R1, R2, and R3, respectively; the second fluid storage element 12 is reagent kit R5; and the third fluid storage element 13 is reagent kit R4. The distribution module 30 uses a first manifold block 31 . Both the first valve 314 and the second valve 315 of the first manifold block 31 are solenoid valves. The first valves 314 are solenoid valves Va, Vb, and Vc, respectively, and the second valve 315 is a solenoid valve Vd. The fluid utilization system 200 uses a flow cell slide C that cooperates with the first manifold block 31 .

[0078] During operation, the reagents or cleaning fluids that do not need to be transported to the flow cell slide C can be directly discharged into the waste liquid bucket 41, which also realizes the pre-infusion of the relevant pipelines to ensure the accuracy of the subsequent steps. Pre-infusion can include the following three methods, and the working logic of each pre-infusion method is as follows:

[0079] First pre-priming method: Referring to FIG7 , the rotary valve 2120 connects the syringe pump 2110 and one of the first fluid storage elements 11 (e.g., reagent cartridge R1). The syringe pump 2110 operates, pumping the reagent from the reagent cartridge R1 and drawing it through the rotary valve 2120 into the fluid transfer line 2130 for temporary storage. The rotary valve 2120 then connects the syringe pump 2110 and the first manifold block 31 via the first input line P1. Simultaneously, the solenoid valve Vd of the first manifold block 31 opens, while the remaining solenoid valves close. The syringe pump 2110 operates, pushing the reagent temporarily stored in the fluid transfer line 2130 through the first fluid inlet 311 into the flow path within the first manifold block 31, and then through the first fluid outlet 313 into the waste liquid tank 41.

[0080] Second pre-priming method: Referring to FIG8 , the syringe pump 2110 is connected to the second fluid storage element 12 (e.g., the reagent cartridge R5). The syringe pump 2110 is activated, pumping the reagent or cleaning solution from the reagent cartridge R5 into the syringe pump 2110 for temporary storage. Then, the rotary valve 2120 is connected to the syringe pump 2110 and to the first manifold block 31 via the first input line P1. Simultaneously, the solenoid valve Vd of the first manifold block 31 is opened, while the remaining solenoid valves are closed. The syringe pump 2110 is activated, pushing the reagent temporarily stored in the syringe pump 2110 through the rotary valve 2120, into the first manifold block 31, and then into the waste liquid tank 41 via the first fluid outlet 313.

[0081] Third pre-priming method: Referring to FIG9 , the plunger pump 2210 is connected to the third fluid storage element 13 (e.g., reagent cartridge R4). The plunger pump 2210 operates, pumping the reagent from the reagent cartridge R4 into the plunger pump 2210 for temporary storage. The plunger pump 2210 then connects to the first manifold block 31. Simultaneously, the solenoid valve Vd of the first manifold block 31 opens, while the remaining solenoid valves close. The plunger pump 2210 operates, pushing the reagent temporarily stored in the plunger pump 2210 through the rotary valve 2120, into the first manifold block 31, and then into the waste liquid tank 41 through the first fluid outlet 313.

[0082] The first pre-infusion method can be executed in parallel with the third pre-infusion method, and the second pre-infusion method can also be executed in parallel with the third pre-infusion method, thereby saving fluid transportation time.

[0083] After pre-infusion, the target reagent can be transported to the flow cell slide C for biochemical reaction. The target reagent transportation can include the following three methods. The working logic of each target reagent transportation method is as follows:

[0084] First target reagent transport method: Referring to Figure 10, the rotary valve 2120 connects the syringe pump 2110 and one of the first fluid storage elements 11 (e.g., reagent cartridge R1). The syringe pump 2110 operates, pumping the reagent from the reagent cartridge R1 and drawing it through the rotary valve 2120 into the fluid transfer line 2130 for temporary storage. The rotary valve 2120 then connects to the syringe pump 2110 and to the first manifold block 31 via the first input line P1. Simultaneously, the solenoid valve Vd of the first manifold block 31 opens, while the remaining solenoid valves close. The syringe pump 2110 operates, pushing the portion of the lower-purity reagent temporarily stored in the fluid transfer line 2130 into the flow channel 310 within the first manifold block 31, and then into the waste liquid tank 41 through the first fluid outlet 313. Then, the solenoid valves Va, Vb, and Vc of the first manifold block 31 are opened, and the other solenoid valves are closed. The injection pump 2110 works, thereby pushing the remaining reagent temporarily stored in the fluid transfer pipeline 2130 into the corresponding opening 201 of the flow cell carrier C through the solenoid valve Va, and the reagent enters the flow channel 310 inside the first manifold block 31 from the other outlets of the flow cell carrier C, and then enters the waste liquid barrel 41 through the first fluid outlet 313.

[0085] Second target reagent transportation method: Referring to Figure 11 , the syringe pump 2110 is connected to the second fluid storage element 12 (e.g., reagent cartridge R5). The syringe pump 2110 operates, pumping the reagent from the reagent cartridge R5 into the syringe pump 2110 for temporary storage. The rotary valve 2120 then connects the syringe pump 2110 and the first manifold block 31 via the first input line P1. Simultaneously, the solenoid valve Vd of the first manifold block 31 opens, while the remaining solenoid valves close. The syringe pump 2110 operates, allowing a portion of the lower-purity reagent temporarily stored in the fluid transfer line 2130 to enter the flow channel 310 within the first manifold block 31 and then enter the waste liquid tank 41 through the first fluid outlet 313. Then, the solenoid valves Va, Vb, and Vc of the first manifold block 31 are opened, and the other solenoid valves are closed. The injection pump 2110 works, thereby pushing the remaining reagent temporarily stored in the fluid transfer pipeline 2130 into the corresponding opening 201 of the flow cell carrier C through the solenoid valve Va, and the reagent enters the flow channel 310 inside the first manifold block 31 from other outlets of the flow cell carrier C, and then enters the waste liquid barrel 41 through the first fluid outlet 313.

[0086] Third target reagent transportation method: Referring to Figure 12 , the plunger pump 2210 is connected to the third fluid storage element 13 (e.g., reagent reagent R4). The plunger pump 2210 operates, pumping the reagent from the reagent reagent R4 into the plunger pump 2210 for temporary storage. The plunger pump 2210 then connects to the first manifold block 31. Simultaneously, the solenoid valve Vd of the first manifold block 31 opens, the remaining solenoid valves close, and the syringe pump 2110 operates, pushing the portion of the lower-purity reagent temporarily stored in the plunger pump 2210 into the flow channel 310 within the first manifold block 31, and then into the waste liquid tank 41 through the first fluid outlet 313. Then, the solenoid valves Va, Vb, and Vc of the first manifold block 31 are opened, and the other solenoid valves are closed. The injection pump 2110 works, thereby pushing the remaining reagent temporarily stored in the fluid transfer pipeline 2130 into the corresponding opening 201 of the flow cell carrier C through the solenoid valve Va, and the reagent enters the flow channel 310 inside the first manifold block 31 from other outlets of the flow cell carrier C, and then enters the waste liquid barrel 41 through the first fluid outlet 313.

[0087] The first target reagent transport mode can be executed in parallel with the third target reagent transport mode. For example, the step of discharging the low-purity reagent into the waste liquid bucket 41 in the first target reagent transport mode and the step of discharging the low-purity reagent into the waste liquid bucket 41 in the third target reagent transport mode can be executed in parallel. The second target reagent transport mode can also be executed in parallel with the third target reagent transport mode. For example, the step of discharging the low-purity reagent into the waste liquid bucket 41 in the second target reagent transport mode and the step of discharging the low-purity reagent into the waste liquid bucket 41 in the third target reagent transport mode can be executed in parallel, thereby saving fluid transportation time.

[0088] Furthermore, before transporting the target reagent to the flow cell slide C for biochemical reaction, the first working module 21 and the second working module 22 can be perfused with each other to mix the fluid in the first fluid storage element 11 and the fluid in the second fluid storage element 12. Assume that before the perfusion, the pipeline between the rotary valve 2120 and the first manifold block 31 is filled with buffer, the pipeline between the rotary valve 2120 and the first fluid storage element 11 is filled with air, the pipeline between the syringe pump 2110 and the second fluid storage element 12 and the rotary valve 2120 is filled with buffer, and the pipeline between the plunger pump 2210 and the third fluid storage element 13 is filled with air. The operating logic of the perfusion method between the working modules 20 is as follows:

[0089] The working modules 20 are priming each other: Referring to Figure 13 , a rotary valve 2120 connects the syringe pump 2110 and one of the first fluid storage elements 11 (e.g., reagent cartridge R1). The syringe pump 2110 operates, pumping the reagent from the reagent cartridge R1 through the rotary valve 2120 and into the fluid transfer line 2130 for temporary storage. The rotary valve 2120 then connects the syringe pump 2110 and, via the first input line P1, the first manifold block 31. Simultaneously, the solenoid valve Vd of the first manifold block 31 opens, while the remaining solenoid valves close. The syringe pump 2110 then operates, pushing the lower-purity reagent temporarily stored in the fluid transfer line 2130 into the flow channel 310 within the first manifold block 31, and then into the waste liquid tank 41 through the first fluid outlet 313. Then, the rotary valve 2120 is connected to the syringe pump 2110 and another first fluid storage element 11 (such as the reagent box R2). The syringe pump 2110 is operated so that the remaining reagent temporarily stored in the fluid transfer line 2130 enters the reagent box R2 through the rotary valve 2120. The above steps can be repeated until a specific volume of fluid (such as reagent 1) is transferred from the reagent box R1 to the reagent box R2 and coexists with a different type of fluid (such as reagent 2) stored in the reagent box R2. Then, the reagent 1 and reagent 2 in the reagent box R2 are controlled to mix uniformly. For example, the syringe pump 2110, the rotary valve 2120 and the reagent box R2 can be connected, and the syringe pump 2110 can be controlled to reciprocate to mix the reagent 1 and reagent 2 in the reagent box R2. Alternatively, the motion control module 60 can be used to drive the reagent box R2 itself to perform mechanical movement to mix the reagent 1 and reagent 2 in the reagent box R2.

[0090] Furthermore, before the previous biochemical reaction is complete, the first working module 21 can transfer the fluid stored in the first fluid storage element 11 to the second working module 22 for temporary storage, thereby freeing up the first working module 21 for pre-pumping the next reagent. That is, after temporarily storing the fluid in the second working module 22, the first working module 21 can pre-pump the next reagent in advance, saving time. The working logic of the fluid temporary storage method is as follows:

[0091] Fluid Temporary Storage Method: Referring to Figure 14, a rotary valve 2120 connects the syringe pump 2110 and one of the first fluid storage elements 11 (e.g., reagent cartridge R2). When the syringe pump 2110 is activated, the reagent is pumped from the reagent cartridge R2 and passed through the rotary valve 2120 into the fluid transfer line 2130 for temporary storage. The rotary valve 2120 connects the syringe pump 2110 and, via the first input line P1, to the first manifold block 31. Simultaneously, the solenoid valve Vd of the first manifold block 31 is opened, while the remaining solenoid valves are closed. The syringe pump 2110 operates, pushing a portion of the lower-purity reagent temporarily stored in the fluid transfer line 2130 into the first input line P1. This lower-purity reagent and the buffer solution at the downstream end are then pushed into the internal flow channel 310 of the first manifold block 31 and then into the waste liquid tank 41 through the first fluid outlet 313. Then, plunger pump 2210 connects to first manifold block 31. All solenoid valves in first manifold block 31 are closed, and plunger pump 2210 operates, pumping the remaining high-purity reagent in first input pipeline P1 into second input pipeline P2 for temporary storage. Rotary valve 2120 then connects syringe pump 2110 and the remaining first fluid storage elements 11, and syringe pump 2110 operates to pre-pump the next reagent.

[0092] Since a single, larger-volume reagent can be transported separately through the second working module 22, the second input pipeline P2 can have a larger volume. As the first input pipeline P1 serves as a common pipeline for different fluids, the first input pipeline P1 can have a smaller volume to avoid fluid waste (when transporting different reagents, the amount of reagent needs to be increased to replace the previous reagent remaining in the common pipeline, resulting in fluid waste). Therefore, in some embodiments, the volume of the second input pipeline P2 is set to be larger than that of the first input pipeline P1, so that the second input pipeline P2 can be suitable for temporarily storing large amounts of reagents.

[0093] Example 2

[0094] Referring to Figure 15 , Example 2 employs the first operating logic for fluid transport, with a first operating module 21 in operation. To simplify the diagram, the inoperative modules in Example 1 have been removed from Figure 16 . The fluid selection assembly 212 of the first operating module 21 includes a rotary valve 2120 and a multi-axis motion-capable liquid extraction needle 2121 connected to the rotary valve 2120. The first power assembly 211 utilizes a syringe pump 2110, and the fluid transfer assembly 213 comprises a fluid transfer line 2130 connected between the rotary valve 2120 and the syringe pump 2110. The fluid storage module 10 includes four first fluid storage elements 11 and one second fluid storage element 12. The four first fluid storage elements 11 are reagent cartridges R1, R2, R3, and R4, and are capable of mechanical movement. The second fluid storage element 12 is reagent cartridge R5, which is fixed in position. The distribution module 30 adopts the first manifold block 31, and the first valve 314 and the second valve 315 of the first manifold block 31 both use solenoid valves. The first valve 314 is Va, Vb, and Vc respectively, and the second valve 315 is Vd. The fluid use module adopts a flow cell slide C that cooperates with the first manifold block 31. In this embodiment, DNB (DNA Nanoball) loading is taken as an example for explanation. Since it is a sensitive reagent, the second fluid inlet 312 of the first manifold block 31 is used to guide it into the flow cell slide C, so that the loading path of DNB is separated from the loading path of other reaction reagents to avoid cross contamination, and at the same time, the solenoid valve is prevented from causing damage to DNB. Assuming that all pipelines and the flow cell slide C are filled with buffer, the working logic of the sensitive reagent transportation method is as follows:

[0095] Sensitive Reagent Transport Method: Referring to Figure 16 , the syringe pump 2110 is connected to the second fluid storage element 12 (e.g., reagent cartridge R5). The syringe pump 2110 operates, pumping the buffer solution from the reagent cartridge R5 into the syringe pump 2110 for temporary storage. The rotary valve 2120 then connects the syringe pump 2110 and, via the first input line P1, to the first manifold block 31. Simultaneously, the solenoid valve Vd of the first manifold block 31 opens, while the remaining solenoid valves close. The syringe pump 2110 operates, allowing the buffer solution temporarily stored in the syringe pump 2110 to pass through the rotary valve 2120, enter the flow path within the first manifold block 31, and then enter the waste liquid bucket 41 through the first fluid outlet 313. This achieves pre-priming of the buffer solution and ensures that the fluid transfer line 2130 is fully filled with the buffer solution. The rotary valve 2120 then connects the syringe pump 2110 to the liquid extraction needle 2121, controlling the liquid extraction needle 2121 to move to the position of the reagent cartridge R1. The syringe pump 2110 is activated, thereby drawing the reagent 1 in the reagent cartridge R1 through the liquid extraction needle 2121 into the pipeline between the liquid extraction needle 2121 and the rotary valve 2120 for temporary storage. The liquid extraction needle 2121 is then controlled to move to the position of the reagent cartridge R2. The syringe pump 2110 is activated, thereby pushing the reagent temporarily stored between the liquid extraction needle 2121 and the rotary valve 2120 into the reagent tank R2. The reagent tank R2 is then rotated to uniformly mix the reagents 1 and 2 coexisting in the reagent tank R2, thereby obtaining the processed reagent DNB (hereinafter referred to as reagent 3).

[0096] Then, referring to Figure 17, the rotary valve 2120 connects the injection pump 2110 and the liquid collection needle 2121, controls the liquid collection needle 2121 to move to the position of the reagent kit R2, and the injection pump 2110 works, so that the reagent 3 in the reagent kit R2 is drawn into the fluid transfer pipeline 2130 through the rotary valve 2120 for temporary storage. Then, the rotary valve 2120 is connected to the injection pump 2110 and connected to the first manifold block 31 through the third input pipeline P3. At the same time, the solenoid valve Vb of the first manifold block 31 is opened, and the other battery valves are closed. The injection pump 2110 works, thereby pushing the reagent with lower purity in the fluid transfer pipeline 2130 into the flow channel 310 in the first manifold block 31 through the third input pipeline P3, and then enters the waste liquid barrel 41 at a low speed through the first fluid outlet 313. During this process, the buffer solution in the third input pipeline P3 is also pushed into the waste liquid barrel 41 at a low speed (at this time, since the flow cell carrier C is filled with buffer solution and the liquid is incompressible, the disturbance is weak at low flow rate, so this part of the discarded fluid will not enter the flow cell carrier C). Then, the solenoid valve Vc of the first manifold block 31 opens, the remaining solenoid valves close, and the syringe pump 2110 operates, pushing the remaining reagent 3 in the fluid transfer line 2130 into the flow cell slide C through the third input line P3. The buffer solution originally in the flow cell slide C flows out through the solenoid valve Vc and the first fluid outlet 313 and into the waste liquid bucket 41. Thus, the loading of the reagent 3 into the flow cell slide C is completed.

[0097] In this embodiment, different reagents are mixed in reagent reservoir R2 and blended to obtain reagent 3. Reagent 3 is then pumped into fluid transfer line 2130 for temporary storage, allowing it to be quickly introduced into flow cell slide C for biochemical reaction. This allows sensitive reagents to be quickly transported to flow cell slide C for biochemical reaction after processing, avoiding quality issues caused by pre-filling the reagent cartridge R2 with sensitive reagents, which could lead to delayed use of the sensitive reagents.

[0098] Referring to Figure 18 , the steps of this embodiment can also be combined with the target reagent transport steps described in Example 1 (e.g., the first target reagent transport method). For example, when a biochemical reaction is ongoing within flow cell slide C, syringe pump 2110 can pre-pump reagent 3 from reagent kit R2 into fluid transfer line 2130 via rotary valve 2120 for temporary storage, and then push the reagent 3 pre-stored in fluid transfer line 2130 into third input line P3. If the biochemical reaction within flow cell slide C is not yet complete, syringe pump 2110 can pre-pump another reagent from reagent kit R1 into fluid transfer line 2130 via rotary valve 2120 for temporary storage, and then push the reagent pre-stored in fluid transfer line 2130 into first input line P1. After the biochemical reaction in the flow cell slide C is completed, the syringe pump 2110 continues to work, and pushes the reagents in the third input pipeline P3 and the first input pipeline P1 into the corresponding openings 201 of the flow cell slide C successively or simultaneously to carry out the next biochemical reaction.

[0099] Example 3

[0100] Referring to Figure 19 , Example 3 employs the fourth operating logic for fluid transport, with a first operating module 21 in operation. To simplify the diagram, the inoperative modules in Example 1 have been omitted from Figure 19 . The fluid selection assembly 212 of the first operating module 21 includes a rotary valve 2120 and multiple liquid extraction needles 2121 connected to the rotary valve 2120. Each liquid extraction needle 2121 corresponds to a first fluid storage element 11 of the fluid storage module 10. The first power assembly 211 utilizes a syringe pump 2110, and the fluid transfer assembly 213 comprises a fluid transfer line 2130 connected between the rotary valve 2120 and the syringe pump 2110. The fluid storage module 10 includes five first fluid storage elements 11 and one second fluid storage element 12. The five first fluid storage elements 11 are reagent kits R1, R2, R3, R4, and R5, respectively. The second fluid storage element 12 is reagent kit R6. The distribution module 30 uses a first manifold block 31 , and both the first valve 314 and the second valve 315 of the first manifold block 31 are solenoid valves. The first valves 314 are Va, Vb, and Vc, respectively, and the second valve is Vd. The fluid use module uses a flow cell slide C that cooperates with the first manifold block 31 .

[0101] The third power assembly 51 of the cleaning and emptying module 50 includes a first diaphragm liquid pump 511 and a second diaphragm liquid pump 512. The cleaning and emptying module 50 also includes a two-position, three-way solenoid valve 52 and a second manifold block 53. The two-position, three-way solenoid valve 52 is disposed on the fluid transfer line 2130, dividing the fluid transfer line 2130 into a first section 2131 and a second section 2132. The first section 2131 of the fluid transfer line 2130 is connected to the rotary valve 2120, while the second section 2132 is connected to the syringe pump 2110. The first diaphragm liquid pump 511 connects the two-position, three-way solenoid valve 52 to the waste module 40. The second manifold block 53 includes a third fluid inlet 531 and multiple second fluid outlets 532. The second diaphragm liquid pump 512 connects the third fluid inlet 531 to the second fluid storage element 12 (reagent box R6). The multiple second fluid outlets 532 are connected to the first fluid storage element 11 (including reagent boxes R1 to R5) via multiple liquid extraction needles 2121. The two-position three-way solenoid valve 52 is used to selectively connect the first section 2131 of the fluid transfer pipeline 2130 to the second section 2132 (ie, to the power pump) or the first diaphragm liquid pump 511 .

[0102] This embodiment can be used to clean internal piping (e.g., the inner wall of the liquid collection needle 2121, and the first valve 314 or the second valve 315 in the first manifold block 31). When cleaning the internal piping, the two-position three-way solenoid valve 52 of the cleaning and emptying module 50 is completely closed, so that the first section 2131 of the fluid transfer pipeline 2130 is connected to the injection pump 2110. The working logic of cleaning the internal piping is as follows:

[0103] Cleaning Internal Pipelines: Referring to Figure 20 , the rotary valve 2120 connects the syringe pump 2110 and the first fluid storage element 11 (e.g., reagent cartridge R5) for storing cleaning fluid. When the syringe pump 2110 is activated, the cleaning fluid is drawn through the rotary valve 2120 into the fluid transfer pipeline 2130 for temporary storage. The rotary valve 2120 then connects the syringe pump 2110 to the first manifold block 31. Simultaneously, the solenoid valve Vd of the first manifold block 31 opens, while the remaining solenoid valves close. The syringe pump 2110 then operates, pushing the cleaning fluid temporarily stored in the fluid transfer pipeline 2130 through the first input pipeline P1 into the flow channel 310 within the first manifold block 31. The fluid then enters the waste liquid tank 41 through the first fluid outlet 313, thereby cleaning the first input pipeline P1 and the upstream pipelines of the waste liquid tank 41. Then, the rotary valve 2120 is connected to the syringe pump 2110 and the reagent kit R5 again. The syringe pump 2110 is activated, allowing the cleaning fluid to be drawn through the rotary valve 2120 into the fluid transfer line 2130 for temporary storage. Then, the rotary valve 2120 is connected to the syringe pump 2110 and each liquid collection needle 2121. The syringe pump 2110 is activated, pushing the cleaning fluid temporarily stored in the fluid transfer line 2130 into the liquid collection needle 2121, thereby cleaning the inner wall of the liquid collection needle 2121. Then, the rotary valve 2120 is connected to the syringe pump 2110 and the reagent kit R5 again. The syringe pump 2110 is activated, allowing the cleaning fluid to be drawn through the rotary valve 2120 into the fluid transfer line 2130 for temporary storage. Then, the solenoid valve of the first manifold block 31 is closed, the other solenoid valves are opened, and the injection pump 2110 works, thereby pushing the cleaning liquid temporarily stored in the fluid transfer pipeline 2130 into the flow channel 310 inside the first manifold block 31 through the first input pipeline P1, and then enters the flow pool carrier C through the solenoid valve Va, and then flows out through the solenoid valves Vb and Vc and enters the waste liquid barrel 41, thereby cleaning the solenoid valves Va, Vb, and Vc.

[0104] Referring to Figure 21 , syringe pump 2110 is connected to the second fluid storage element 12 (e.g., reagent cartridge R6). Syringe pump 2110 operates, drawing cleaning fluid into syringe pump 2110 for temporary storage. Then, rotary valve 2120 connects syringe pump 2110 to first manifold block 31. Simultaneously, solenoid valve Vd in first manifold block 31 opens, while the remaining solenoid valves close. Syringe pump 2110 operates, pushing the cleaning fluid temporarily stored in syringe pump 2110 through first input pipeline P1 into flow channel 310 within first manifold block 31. The fluid then flows through first fluid outlet 313 into waste liquid tank 41, thereby cleaning first input pipeline P1 and the upstream pipeline of waste liquid tank 41. Syringe pump 2110 is then connected to reagent cartridge R6 again and operated, drawing cleaning fluid into syringe pump 2110 for temporary storage. Then, rotary valve 2120 connects syringe pump 2110 and each liquid extraction needle 2121. Syringe pump 2110 operates, pushing the cleaning fluid temporarily stored in syringe pump 2110 into liquid extraction needle 2121, thereby cleaning the inner wall of liquid extraction needle 2121. Syringe pump 2110 is then connected to reagent chamber R6 again and operates, drawing cleaning fluid into syringe pump 2110 for temporary storage. Then, solenoid valve Vd of first manifold block 31 is closed, while the remaining solenoid valves are opened. Syringe pump 2110 operates, pushing the cleaning fluid temporarily stored in fluid transfer line 2130 through first input line P1 into flow channel 310 within first manifold block 31. The cleaning fluid then enters flow cell slide C through solenoid valve Va, then flows out through solenoid valves Vb and Vc and into waste liquid tank 41, thereby cleaning solenoid valves Va, Vb, and Vc.

[0105] Referring to Figure 22, to extend the life of the syringe pump 2110, this embodiment also utilizes the first diaphragm liquid pump 511 to drive and transport cleaning fluid to clean the internal pipelines. Furthermore, since the bidirectional pumping and pushing motions of the syringe pump 2110 are replaced with a unidirectional drive of the first diaphragm liquid pump 511, this helps speed up the cleaning process. The cleaning and emptying module 50 may also include another two-position, three-way solenoid valve 52', which is used to selectively connect the first diaphragm liquid pump 511 to the waste liquid bucket 41 or the first fluid storage element 11 (such as the reagent kit R5) for storing cleaning fluid. When the cleaning and emptying module 50 cleans the internal pipeline, the two-position three-way solenoid valve 52' is completely closed, so that the first diaphragm liquid pump 511 is connected to the reagent box R5. The working logic of the cleaning and emptying module 50 to clean the internal pipeline is: the two-position three-way solenoid valve 52 is opened, so that the first section 2131 of the fluid transfer pipeline 2130 is connected to the first diaphragm liquid pump 511, the two-position three-way solenoid valve 52' is opened, so that the first diaphragm liquid pump 511 is connected to the reagent box R5, and the rotary valve 2120 connects the first section 2131 of the fluid transfer pipeline 2130 and each liquid collection needle 2121. Then, the first diaphragm liquid pump 511 works, so that the cleaning liquid stored in the reagent box R5 passes through the two-position three-way solenoid valves 52, 52' and the rotary valve 2120 and enters the liquid collection needle 2121, thereby cleaning the inner wall of the liquid collection needle 2121.

[0106] In this embodiment, the cleaning and emptying module 50 can also clean the outer wall of the liquid collection needle 2121 at any appropriate point during the biochemical reaction process. By introducing an additional power source (i.e., the first diaphragm liquid pump 511) to clean the outer wall of the liquid collection needle 2121, the life of the syringe pump 2110 can also be saved. The working logic of the cleaning and emptying module 50 for cleaning the external pipeline is as follows:

[0107] Cleaning External Pipes: Referring to Figure 23 , the second diaphragm liquid pump 512 connects to the third fluid inlet 531 of the second manifold block 53 and the second fluid storage element 12 (e.g., reagent cartridge R6). Simultaneously, the second fluid outlet 532 of the second manifold block 53 connects to the first fluid storage element 11 (e.g., reagent cartridges R1 through R5). Operation of the second diaphragm liquid pump 512 pumps cleaning fluid from reagent cartridge R6 into the second manifold block 53, where it flows out through the second fluid outlet 532 of the second manifold block 53, thereby cleaning the outer wall of the liquid extraction needle 2121.

[0108] In this embodiment, after the biochemical reaction is completed, the cleaning and emptying module 50 further operates to empty the internal pipelines of the fluid transport system 100. The working logic of the cleaning and emptying module 50 to empty the internal pipelines is as follows:

[0109] Emptying the Internal Pipes: Referring to Figure 24 , the two-position, three-way solenoid valve 52 is opened, connecting the first section 2131 of the fluid transfer line 2130 to the first diaphragm pump 511. Simultaneously, the rotary valve 2120 selectively connects the first section 2131 of the fluid transfer line 2130 to one of the reagent chambers R1 to R5. The first diaphragm pump 511 operates, pumping the remaining reagent in the connected reagent tank into the waste liquid bucket 41 via the rotary valve 2120, the first section 2131 of the fluid transfer line 2130, the two-position, three-way solenoid valve 52, and the first diaphragm pump 511. This also empties the internal piping of the liquid extraction needle 2121. The rotary valve 2120 then selectively connects the first section 2131 of the fluid transfer line 2130 to another of the reagent chambers R1 to R5. The first diaphragm pump 511 operates again until the internal piping of each reagent chamber and the corresponding liquid extraction needle 2121 are empty.

[0110] Then, referring to Figure 25 , the two-position, three-way solenoid valve 52 is closed, thereby connecting the first section 2131 and the second section 2132 of the fluid transfer line 2130. Simultaneously, the rotary valve 2120 connects the first section 2131 of the fluid transfer line 2130 to any emptied liquid extraction needle 2121. The syringe pump 2110 connects the second section 2132 of the fluid transfer line 2130 and draws air in. Next, the rotary valve 2120 connects the first section 2131 of the fluid transfer line 2130 to the first manifold block 31. Simultaneously, the solenoid valve Vd of the first manifold block 31 is opened, while the remaining solenoid valves are closed. The syringe pump 2110 operates, pushing some of the drawn air through the first input line P1 into the flow channel 310 within the first manifold block 31, thereby draining the solenoid valve Vd. Then, the solenoid valves Va, Vb, and Vc of the first manifold block 31 are opened, and the syringe pump 2110 is operated, thereby pushing the drawn-in remaining air into the flow cell slide C, thereby emptying the solenoid valves Va, Vb, and Vc.

[0111] Referring to FIG. 26 , one embodiment of the present application further provides a method for fluid transport, which can be applied to the above-mentioned fluid transport system 100 . The order of the steps of the above-mentioned method can be changed according to different requirements, and some steps can be omitted or combined. The above-mentioned fluid transport method includes the following steps:

[0112] Step S1 : establishing a connection between the fluid storage module 10 , the first working module 21 , and the second working module 22 .

[0113] In step S2 , the first power component 211 is activated, thereby selecting a fluid from the fluid storage module 10 through the fluid selection component 212 and driving the selected fluid to be transferred to the fluid use system 200 .

[0114] In step S3 , the second power assembly 221 is activated to drive another fluid in the fluid storage module 10 to be transferred to the fluid using system 200 .

[0115] It is understood that the fluid loading method of the present application is not limited to the above steps. Through the cooperation of the various modules of the fluid transport system 100, other steps can also be performed to achieve different functions of the fluid transport system 100. Please refer to the four working logics listed above for details, and no further details will be repeated here.

[0116] Please refer to FIG. 27 . One embodiment of the present application further provides a gene sequencer 1 , which includes the fluid transport system 100 or is used to execute the above-mentioned fluid transport method.

[0117] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and are not intended to limit the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A fluid transport system for transporting fluid to a fluid-using system, characterized in that: The fluid transport system comprises: A fluid storage module, used for storing a variety of fluids; A first working module, comprising a fluid selection component and a first power component; and A second working module includes a second power assembly; At least one of the first working module and the second working module is selectively connected to the fluid use system, the fluid selection component is used to select a fluid from the fluid storage module, the first power component is used to drive the selected fluid to be transferred to the fluid use system, and the second power component is used to drive another fluid in the fluid storage module to be transferred to the fluid use system.

2. The fluid transport system according to claim 1, wherein: The fluid transport system further comprises: A distribution module is used to connect to the fluid use system. Two first fluid inlets are provided on the distribution module. One of the first fluid inlets is connected to the fluid selection component through a first input pipeline, and the other first fluid inlet is connected to the second power component through a second input pipeline. The distribution module is also provided with multiple slide interfaces, and the multiple slide interfaces are respectively arranged corresponding to the multiple openings of the fluid use system. One of the slide interfaces is connected to the first fluid inlet through a first valve.

3. The fluid transport system according to claim 2, wherein: The second input pipeline is used to temporarily store the fluid transported from the first input pipeline. After the second input pipeline temporarily stores the fluid, the fluid selection component is further used to select fluid again from the fluid storage module.

4. The fluid transport system according to claim 3, wherein: The volume of the second input pipeline is greater than the volume of the first input pipeline.

5. The fluid transport system according to claim 3, wherein: The first power assembly is further used to drive the fluid selected by the fluid selection assembly to be transferred to the fluid use system, and the second power assembly is further used to drive the fluid temporarily stored in the second input pipeline to be transferred to the fluid use system.

6. The fluid transport system according to claim 2, wherein: The distribution module is also provided with a second fluid inlet, which is connected to the fluid selection component through a third input pipeline, and a slide interface is connected to the second fluid inlet, and the fluid path between the second fluid inlet and the corresponding slide interface does not pass through the first valve.

7. The fluid transport system according to claim 2, wherein: The distribution module further includes a first fluid outlet, and the fluid transport system further includes a waste liquid module in communication with the first fluid outlet, wherein the waste liquid module is configured to store the fluid flowing out of the first fluid outlet.

8. The fluid transport system according to claim 2, wherein: The fluid selection assembly includes a rotary valve and a liquid extraction needle connected to the rotary valve. The rotary valve is used to extract a fluid from the fluid storage module through the liquid extraction needle.

9. The fluid transport system according to claim 8, wherein: The liquid extraction needle is used to perform mechanical motion to move to a corresponding position of the fluid storage module to extract a fluid from the fluid storage module.

10. The fluid transport system according to claim 8, wherein: The fluid storage module is used to perform mechanical movement to align the liquid extraction needle with a corresponding position of the fluid storage module, so that the liquid extraction needle extracts a fluid from the fluid storage module.

11. The fluid transport system according to claim 8, wherein: The fluid transport system further comprises: The cleaning and emptying module includes a third power component, which is used to connect to the fluid storage module. The fluid stored in the fluid storage module includes cleaning fluid. The third power component is also used to connect to the first power module, the second power module and at least one of the distribution modules, and drive the cleaning fluid to flow through at least one of the first power module, the second power module and the distribution module.

12. The fluid transport system according to claim 11, wherein: The cleaning and emptying module also includes a second manifold block, which includes a third fluid inlet connected to the third power assembly and a second fluid outlet arranged corresponding to the outer wall of the liquid extraction needle. The third power assembly is also used to drive the cleaning fluid to flow through the second manifold block and the outer wall of the liquid extraction needle.

13. The fluid transport system according to claim 7, wherein: The fluid transport system further comprises: The cleaning and emptying module includes a third power assembly, wherein the third power assembly is used to connect the fluid storage module and the waste liquid module and drive the fluid in the fluid storage module to flow out and into the waste liquid module.

14. The fluid transport system according to claim 1, wherein: The fluid storage module includes at least two first fluid storage elements, each of which is connected to the fluid selection component and is used to store different fluids. When the fluid selection component selects a fluid from one of the first fluid storage elements, the first power component is also used to drive the selected fluid to be transferred to another of the fluid storage elements.

15. The fluid transport system according to claim 14, wherein: The fluid storage module is used to perform mechanical motion to uniformly mix different fluids in the same first fluid storage element.

16. The fluid transport system according to claim 1, wherein: The first working module and the second working module are used to work in parallel.

17. A fluid transport method for transporting fluid to a fluid-using system, characterized in that: The fluid transport method comprises: Establishing a connection between the fluid storage module and a first working module and a second working module, wherein the first working module includes a fluid selection component and a first power component, and the second working module includes a second power component; The first power assembly is activated to select a fluid from the fluid storage module through the fluid selection assembly and drive the selected fluid to be transferred to the fluid use system; and The second power assembly is activated to drive another fluid in the fluid storage module to be transferred to the fluid using system.

18. The fluid transport method according to claim 17, wherein: Before starting the first power assembly or the second power assembly, the fluid transportation method further includes: Establishing a connection between a dispensing module and the fluid use system, wherein the dispensing module is provided with a plurality of slide interfaces, the plurality of slide interfaces being respectively provided corresponding to the plurality of openings of the fluid use system, and the dispensing module is further provided with two first fluid inlets, one of the slide interfaces being connected to the first fluid inlet via a first valve; opening the first valve; Connecting a first fluid inlet to the fluid selection assembly via a first input line, so that the first power assembly drives the corresponding fluid to be transferred to the fluid use system through the first input line, the first fluid inlet, and the corresponding slide interface; Connect another first fluid inlet to the second power assembly through a second input pipeline, so that the second power assembly drives the corresponding fluid through the second input pipeline, the first fluid inlet and the corresponding The slide interface is transferred to the fluid handling system.

19. The fluid transport method according to claim 18, wherein: When the fluid reacts within the fluid-using system, the fluid transport method further comprises: The first power component selects fluid again from the fluid storage module through the fluid selection component, and drives the reselected fluid to be transferred from the first input pipeline to the second input pipeline, so that the second input pipeline temporarily stores the fluid; The first power assembly selects fluid from the fluid storage module for a third time through the fluid selection assembly.

20. The fluid transport method according to claim 19, wherein: When the reaction is completed in the fluid-using system, the fluid transport method further comprises: The first power assembly drives a third selected fluid to be transferred to the fluid using system; The second power assembly drives the fluid temporarily stored in the second input pipeline to be transferred to the fluid using system.

21. The fluid transport method according to claim 19, wherein: The volume of the second input pipeline is greater than the volume of the first input pipeline.

22. The fluid transport method according to claim 18, wherein: The distribution module is further provided with a second fluid inlet, and the fluid transport method further comprises: The second fluid inlet is connected to the fluid selection component through a third input pipeline, and the carrier interface is connected to the second fluid inlet, so that the first power component drives the corresponding fluid to be transferred to the fluid use system through the third input pipeline, the second fluid inlet and the corresponding carrier interface, and the fluid path between the second fluid inlet and the corresponding carrier interface does not pass through the first valve.

23. The fluid transport method according to claim 22, wherein: When the fluid reacts within the fluid-using system, the fluid transport method further comprises: The first power component selects fluid again from the fluid storage module through the fluid selection component, and drives the reselected fluid to be transferred to the third input pipeline; The first power assembly selects fluid from the fluid storage module for a third time through the fluid selection assembly, and drives the third selected fluid to be transferred to the first input pipeline.

24. The fluid transport method according to claim 23, wherein: When the reaction is completed in the fluid-using system, the fluid transport method further comprises: The first power assembly drives the fluid in the third input pipeline to be transferred to the fluid using system; The first power assembly drives the fluid in the first input pipeline to be transferred to the fluid using system.

25. The fluid transport method according to claim 17, wherein: The fluid selection component includes a rotary valve and a liquid collection needle connected to the rotary valve. The first power component is activated, so that the fluid selection component selects a fluid from the fluid storage module, specifically comprising: The fluid selection component extracts a fluid from the fluid storage module through the liquid extraction needle.

26. The fluid transport method according to claim 25, wherein: Before the liquid extraction needle extracts the fluid, the liquid extraction needle performs mechanical motion to move to a corresponding position of the fluid storage module to extract the fluid from the fluid storage module.

27. The fluid transport method according to claim 25, wherein: Before the liquid extraction needle extracts the fluid, the fluid storage module performs mechanical movement to align the liquid extraction needle with a corresponding position of the fluid storage module, so that the liquid extraction needle extracts the fluid from the fluid storage module.

28. The fluid transport method according to claim 18, wherein: After transferring the fluid to the fluid-using system, the fluid transport method further comprises: Providing a cleaning and emptying module, the cleaning and emptying module includes a third power assembly; Establishing a connection between the third power assembly and the fluid storage module, wherein the fluid stored in the fluid storage module includes a cleaning fluid, and establishing a connection between the third power assembly and at least one of the first power module, the second power module, and the distribution module; The third power assembly is activated to drive the cleaning fluid to flow through at least one of the first power module, the second power module, and the distribution module.

29. The fluid transport method according to claim 18, wherein: After transferring the fluid to the fluid-using system, the fluid transport method further comprises: Providing a cleaning and emptying module, the cleaning and emptying module includes a third power assembly; Establishing a connection between the third power assembly and the fluid storage module and the waste liquid module; The third power assembly is activated to drive the fluid in the fluid storage module to flow out and into the waste liquid module.

30. The fluid transport method according to claim 29, wherein: After driving the fluid in the fluid storage module into the waste liquid module, the fluid transportation method further comprises: Establishing a connection between the first working module, the fluid storage module, and the distribution module; The first power assembly is activated to draw air from the fluid storage module through the fluid selection assembly and drive the air to be transferred to the distribution module and the fluid use system.

31. The fluid transport method according to claim 17, wherein: The fluid storage module includes at least two first fluid storage elements, each of which is connected to the fluid selection component and is used to store different fluids. When the fluid selection component selects a fluid from one of the first fluid storage elements, the first power component also drives the selected fluid to be transferred to another of the fluid storage elements.

32. The fluid transport method according to claim 31, wherein: The fluid storage module is used to perform mechanical motion to uniformly mix different fluids in the same first fluid storage element.

33. The fluid transport method according to claim 17, wherein: The first power assembly utilizes negative pressure to extract fluid from the fluid storage module through the fluid selection assembly, and utilizes positive pressure to push the selected fluid to be transferred to the fluid use system.

34. The fluid transport method according to claim 33, wherein: Before using positive pressure to push the selected fluid to be transferred to the fluid use system, the first power component temporarily stores the extracted fluid in a fluid transfer component, and the fluid transfer component is connected between the first power component and the fluid selection component, or the fluid transfer component is a cavity within the first power component.

35. A gene sequencer, characterized in that A fluid transport system comprising the fluid transport system according to any one of claims 1 to 16, or used for performing the fluid transport method according to any one of claims 17 to 34.

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